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| 1 | Physical Location of the Rice Pi-5(t), Glh and RTSV Genes by ISH of BAC Clones显示文摘Mapplng of low of slngle-copy sequences on plant chromosomes has proven dirricult because ofvery low frequency or slgnal detectlon. Rlce BAC Ilbrary ls belng used wldely ln rlce genome research due tolts distlnctlve advantages over other llbrary systems. In tkls study, two blotln-Iabeled rice BAC clones closelylinked to a rlce blast reslstance, green Ieaf hopper reslstance and tungro spherlcal virus resistance gene,Pi-5(t),Glh, RTSV, were in situ hybrldlred to rlce ckromosomes. They were located on the long arm andshort arm of ckromosome 4 with FL value of 40% and 100% respectively-The rrequency of signal detectionreacked 46. 8% and 59- 2%- The slgnal location were conslstent wltk the selectlve marker on rice saturatedm0Iecular map. The results demonstrated the advautages to Iocate BAC cIones to ckromosomes by in sho hybrldization and will racilitate tke rlce low of slngle-copy gene Iocation by using the BAC library. | Yan Huimin, Song Yunchun, Li Lijia, Bi Xuezhi, Fu Binying(College of Life Sciences, Wuhan University, Wuhan 430072, China) | 1998 | Wuhan University Journal of Natural Sciences1998，3，2： | 24 |
| 2 | Propofol differentially induces unconsciousness and respiratory depression through distinct interactions between GABAa receptor and GABAergic neuron in corresponding nuclei显示文摘Propofol is the most commonly used intravenous anesthetic worldwide.It can induce loss of consciousness prior to the occurrence of severe respiratory suppression,which is also a pharmacodynamic feature of all general anesthetics.However,the neural mechanisms underlying this natural phenomenon are controversial and highly related to patient safety.In the present study,we demonstrated that the pharmacodynamic effects of propofol(50 and 100 μM)on suppression of consciousness-related excitatory postsynaptic currents in the medial prefrontal cortex(mPFC)and centromedian nucleus of the thalamus(CMT)were lower than those in the kernel respiratory rhythmogenesis nucleus pre-Botzinger complex(PrBo).Furthermore,we unexpectedly found that the GABAa receptor β3 subunit is the key target for propofol's action and that it is mutually and exclusively expressed in GABAergic neurons.It is also more abundant in the mPFC and CMT,but mainly co-localized with GABAergic neurons in the PrBo.As a result,the differentiated expression pattern should mediate more neuron suppression through the activation of GABAergic neurons in the mPFC and CMT at low doses of propofol(50 μM).However,PrBo GABAergic neurons were only activated by propofol at a high dose(100 μM).These results highlight the detailed pharmacodynamic effects of propofol on consciousness-related and respiration-related nuclei and provide the distinct interaction mechanism between the β3 subunit and GABAergic neurons in mediating the suppression of consciousness compared to the inhibition of respiration. | Junli Jiang Yingfu Jiao Po Gao Wen Yin Wei Zhou Yunchun Zhang Yanjun Liu Daxiang Wen Yuan Wang Liang Zhou Tian Yu Weifeng Yu | 2021 | Acta Biochimica et Biophysica Sinica2021，53，8： | 11 |
| 3 | Effect of aging on the mechanical properties and corrosion susceptibility of an Al-Cu-Li-Zr alloy containing Sc显示文摘Al-3.5Cu-1.5Li-0.22 (Sc+Zr ) 合金的微观结构,机械性质, intergranular 腐蚀,和脱落腐蚀上的老化的效果被调查了。结果证明合金有年龄变硬,和降水的主要阶段的特性,加强是 T1 阶段。变老的温度和老化时间在 T1 阶段的数量和分发上有重要效果。合金的合适的人工的单个老化的处理在 160r_ | LIANG Wenjie PAN Qinglin HE Yunbin LI Yunchun ZHOU Yingchun LU Congge | 2008 | Rare Metals2008，27，2： | 7 |
| 4 | Physical location of rice Gm-6,Pi-5(t) genes in O.officinalis with BAC-FISH显示文摘A fluorescence in situ hybridization (FISH) procedure was adopted to physically map two rice BAC clones 24E21 and 4F22 linked to Gm-6 and Pi-5(t) in O. offi-cinalis. FISH results showed that the two BAC clones were located at 4L. The percentage distance from the centromere to the hybridization sites was 72 ± 2.62 for 24E21 and 54± 5.43 for 4F22, the detection rates were 52.70% and 61.2%. The results obtained from the BAC and plasmid clones, RG214 and RZ565 of cultivated rice and O. officinalis were the same. This suggested that the markers, RG214 and RZ565 of cultivated rice and O. officinalis were in the same BAC clones. The homologous sequences of Gm-6 and Pi-5(t) in O. officinalis were positions that signals existed on the 4L. Many signals were observed when no Cot-1 DNA blocked. This also showed that repetitive sequences were some ho-molgous between cultivated rice and O. officinalis. The identification of chromosome 4 of O. officinalis is based on Jena et al. (1994). In our study, we discussed | QIN Rui WEI Wenhui JIN Weiwei HE Guangcun NING Shunbin YU Shunwu SONG Yunchun | 2001 | Chinese Science Bulletin2001，46，8： | 6 |
| 5 | Comparative genome research between maize and rice using genomic in situ hybridization显示文摘Using the genomic DNAs of maize and rice as probes respectively, the homology of maize and rice genomes was assessed by genomic in situ hybridization. When rice genomic DNAs were hybridized to maize, all chromosomes displayed many multiple discrete regions, while each rice chromosome delineated a single consecutive chromosomal region after they were hybridized with maize genomic DNAs. The results indicate that the genomes of maize and rice share high homology, and confirm the proposal that maize and rice are diverged from a common ancestor. | Shunbin Ning Weiwei Jin Ling Wang Yi Ding Yunchun Song | 2001 | Chinese Science Bulletin2001，46，8： | 5 |
| 6 | Logging Evaluation of the Ordovician Carbonate Reservoir Beds in the Lungudong Region,Tarim Basin显示文摘在最近的年里,大进步在 Tarim 盆的 Lungudong 区域在油和煤气的探索常常被取得了。然而,进步在它的主要生产油的地平线的评估是慢的奥陶纪碳酸盐水库床。基于以前的研究并且在象钻,地质学和油测试那样的各种各样的数据上,在有各单个井的成像和常规记载数据的解释的联合,并且通过分析和比较,在成像并且在这个区域的为碳酸盐水库床的四种类型的常规记载的鉴定方法在这篇论文被总结。为四个水库床参数的计算公式,我。e。在这个区域的页岩内容,孔,渗透和油浸透被建议;并且在这个区域的水库床被划分成三个层次(我, II 和 III ) 由联合油测试数据并且记载数据,水库床的有效的孔的更低的限制和有效水库的破裂孔,床分别地作为 1.8% 和 0.04% 被决定。物理性质参数被常规记载的曲线计算,并且为水库开发的最有益的区域包括地被预言。在飞机上,水库床参数的高值的地区主要在 N-S-trending 罢工滑倒差错被集中, Sangtamu 差错 horst 地区并且接近 LG38 很好区域;垂直地, Yijianfang 形成的水库床参数比 Yingshan 和 Lianglitage 形成的那些好。 | YANG Wenjing XIAO Chengwen LIU Luofu QI Guangzhong JIANG Zhenxue YUAN Yunchun WANG Weili YANG song | 2010 | Acta Geologica Sinica(English Edition)2010，84，5： | 5 |
| 7 | Determination of copy number for 5S rDNA and centromeric sequence RCS2 in rice by Fiber-FISH显示文摘The copy number of 5S rDNA and centromerie sequence RCS2 was determined by extended DNA fiber based fluorescence in situ hybridization (Fiber-FISH) in rice (Oryza sativa ssp. indica cv. Guangluai No. 4) genome. In order to determine the copy number, it is necessary to know the basepair number that a given length DNA fiber contains under a microscope. Therefore, the length of two DNA frag-ments, in which the basepair number had been already known, was measured. The insert sequence of the tested BAC 38D17 was 136 kb and its extended DNA was 56.4 μm long, 2.41 kb/μm on average, while that of the tested BAC 44B4 was 144.5 kb in total and 55.7 μm long, 2.60 kb/μm on average under the microscope. They were very close to the theoretical value of B-DNA in the Watson-Crick DNA model, which is 2.97 kb/μm. According to the average value of basepair number per μm of the two samples mentioned above, that is, 2.51 kb/μm, it could be estimated that the copy number was about 686 for 5S rDNA and 286-1121 for the | Zongyun Li Siluo Huang Weiwei Jin Shunbin Ning Yunchun Song Lijia Li | 2002 | Chinese Science Bulletin2002，47，3： | 5 |
| 8 | A novel method for in situ detection of apoptotic cell death in plants显示文摘A simple novel protocol suitable for in situ detection of apoptotic plant cell death is presented. This protocol combines the chromosome spreading technique with an extended in situ end labeling version for plants and makes it possible to detect apoptosis directly at chromosome,nuclear and DNA levels simultaneously with high-sensitivity. False positive and nonspecific signals can be efficiently avoided. Moreover, the changes of chromosomes, nuclei and DNA during the process of apoptosis can be characterized in situ. To illustrate this method, salt stress-induced cell death has been investigated in maize root meristematic cells. | Shunbin Ning Yunchun Song Ling Wang Lihua Liu | 1999 | Chinese Science Bulletin1999，44，11： | 4 |
| 9 | The mechanics and deformation of high temperature steel frame rapidly cooled by spray water in fire fighting显示文摘A finite element is established for analyzing the dynamical mechanics and deformation of steel frame at high temperature when it is rapidly cooled down by spray water in fire fighting. The simulation result shows that remarkable mechanical coupling effects are produced in the process, and the sectional stress in rapid cooling down is found considerably larger than that in heating-up. Meanwhile, the stress and deformation of a beam mainly related to cooling rate and location are much larger than those of a column in rapid cooling. In fire fighting, the structure on the first or second floor was more dangerous than those on other floors in rapid cooling. These results could provide a theoretical reference for the design of steel structure and fire fighting. | Yunchun Xia | 2016 | Theoretical & Applied Mechanics Letters2016，6，4： | 3 |
| 10 | Mouse Karyotype Obtained by Combining DAPI Staining with Image Analysis显示文摘In this study, mitotic metaphase chromosomes in mouse were identified by a new chromosome fluorescence banding technique combining DAPI staining with image analysis. Clear 4’, 6-diamidino-2-phenylindole (DAPI) multiple bands like G-bands could be produced in mouse. The MetaMorph software was then used to generate linescans of pixel intensity for the banded chromosomes from short arm to long arm. These linescans were sufficient not only to identify each individual chromosome but also analyze the physical sites of bands in chromosome. Based on the results, the clear and accurate karyotype of mouse metaphase chromosomes was established. The technique is therefore considered to be a new method for cytological studies of mouse. | DAI Xiaohua YANG Guangxu LIU Jingyu SONG Yunchun | 2006 | Wuhan University Journal of Natural Sciences2006，11，2： | 3 |
| 11 | Polymorphisms of potential drug resistant molecular markers in Plasmodium vivax from China-Myanmar border during 2008-2017显示文摘Background:Plasmodium vivax remains the predominant species at the China–Myanmar border,imposing a major challenge to the recent gains in regional malaria elimination.To closely supervise the emerging of drug resistance in this area,we surveyed the variations in genes potentially correlated with drug resistance in P.vivax parasite and the possible drug selection with time.Methods:A total of 235 P.vivax samples were collected from patients sufering uncomplicated malaria at Yingjiang,Tengchong,and Longling counties,and Nabang port in China,Yunnan province,and Laiza sub-township in Myanmar,from 2008 to 2017.Five potential drug resistance genes were amplifed utilizing nested-PCR and analyzed,including pvdhfr,pvdhps,pvmdr1,pvcrt-o,and pvk12.The Pearson’s Chi-squared test or Fisher’s exact test were applied to determine the statistical frequency diferences of mutations between categorical data.Results:The pvdhfr F57I/L,S58R,T61M and S117T/N presented in 40.6%,56.7%,40.1%,and 56.0% of the sequenced P.vivax isolates,and these mutations signifcantly decreased with years.The haplotype formed by these quadruple mutations predominated in Yingjiang,Tengchong,Longling and Nabang.While a mutation H99S/R(56.6%)dominated in Laiza and increased with time.In pvdhps,the A383G prevailed in 69.2% of the samples,which remained the most prevalent haplotype.However,a signifcant decrease of its occurrence was also noticed over the time.The S382A/C and A553G existed in 8.4% and 30.8% of the isolates,respectively.In pvmdr1,the mutation Y976F occurred at a low frequency in 5/232(2.2%),while T958M was fxed and F1076L was approaching fxed(72.4%).The K10 insertion was detected at an occurrence of 33.2% in pvcrt-o,whereas there was no signifcant diference among the sites or over the time.No mutation was identifed in pvk12.Conclusions:Mutations related with resistance to antifolate drugs are prevalent in this area,while their frequencies decrease signifcantly with time,suggestive of increased susceptibility of P.vivax parasite to antifolate drugs.Resistance to chloroquine(CQ)is possibly emerging.However,since the molecular mechanisms underneath CQ resistance is yet to be better understood,close supervision of clinical drug efciency and continuous function investigation is urgently needed to alarm drug resistance. | Zhensheng Wang Chunyan Wei Yunchun Pan Zhihua Wang Xin Ji Qianqian Chen Lianhui Zhang Zenglei Wang Heng Wang | 2022 | Infectious Diseases of Poverty2022，11，2： | 2 |
| 12 | Dexmedetomidine maintains blood-brain barrier integrity by inhibiting Drp1-related endothelial mitochondrial dysfunction in ischemic stroke显示文摘Stroke is the second leading cause of death and long-term disability worldwide,which lacks effective treatment.Perioperative stroke is associated with much higher rates of mortality and disability.The neuroprotective role of dexmedetomidine(Dex),a highly selective agonist of alpha2-adrenergic receptor,has been reported in a stroke rat model,and it was found that pretreatment of Dex before stroke could alleviate blood–brain barrier(BBB)breakdown.However,the underlying mechanisms are still unknown.As the brain endothelial cells are the main constituents of BBB and in high demand of energy,mitochondrial function of endothelial cells plays an important role in the maintenance of BBB.Given that dynamin-related protein 1(Drp1)is a protein mediating mitochondrial fission,with mitochondrial fusion that balances mitochondrial morphology and ensures mitochondria function,the present study was designed to investigate the possible role of Drp1 in endothelial cells involved in the neuroprotective effects of Dex in ischemic stroke.Our results showed that preconditioning with Dex reduced infarction volume,alleviated brain water content and BBB damage,and improved neurological scores in middle cerebral artery occlusion rats.Meanwhile,Dex enhanced cell activity and decreased cell apoptosis in oxygen-glucose deprivation human brain microvascular endothelial cells in vitro.These protective effects of Dex were correlated with the mitochondrial morphology integrality of endothelial cells,mediated by increased phosphorylation of serine 637 in Drp1,and could be reversed byα2-adrenergic receptor antagonist Yohimbine and AMP-activated protein kinase inhibitor Compound C.These findings suggest new molecular pathways involved in the neuroprotective effects of Dex in ischemic stroke.As Dex is routinely used as a sedative drug clinically,our findings provide molecular evidence that it has perioperative neuroprotection from ischemic stroke. | Wei Zhou Yunchun Zhang Yingfu Jiao Wen Yin Haiping Dong Saihong Xu Dan Tang Junli Jiang Jianlin Shao Zhenhong Wang Weifeng Yu | 2021 | Acta Biochimica et Biophysica Sinica2021，53，9： | 2 |
| 13 | Introgression of perennial teosinte genome into maize and identification of genomic in situ hybridization and microsatellite markers显示文摘 | Qilin Tang Tingzhao Rong Yunchun Song | 2005 | Crop Science2005，45，： | 1 |
| 14 | Thickness-shear mode acoustic sensor for atrazine using molecularly imprinted polymer as recongnition element显示文摘 | LUO Chunhua LIU Mengqin MO Yunchun | 2001 | Anal Chim Acta2001，428，1： | 1 |
| 15 | Blend Films of Chitosan/Starch显示文摘BlendFilmsofChitosan/StarchDuYumin,XiaZuyong,LuRongDepartmentofEnvironmentalScience,WuhanUniversity,Wuhan430072,ChinaAbstract... | Song Yunchun Ren Nan Mao Ninghui Liu Lihua | 1997 | Wuhan University Journal of Natural Sciences1997，2，2： | 1 |
| 16 | Farrell,''Two Antennas GPS-Aided INS for Attitude Determination显示文摘 | Yunchun Yang Jay A | 2003 | IEEE Transactions on Control Systems Technology2003，11，： | 1 |
| 17 | Strong convergence of iteration algorithms for a countable family of nonexpansive mappings显示文摘 | SONG Yisheng ZHENG Yunchun | 2009 | Nonlinear Analysis:Theory Methods & Application2009，71，7： | 1 |
| 18 | Thickness-shear mode acoustic sensor for atrazine using molecularly imprinted polymer as recognition element显示文摘 | Luo Chunhua Liu Megqin Mo Yunchun | 2001 | Anal Chim Acta2001，428，1： | 1 |
| 19 | Species Diversity Based on Vertical Structure as Indicators of Artificial Restoration for Coniferous Forests in Southwest China显示文摘Five indices of species richness, species diversity and species evenness were used to assess α diversity of four types of coniferous forests undergone different artificial regeneration time. The study was based on the vertical structure of forests, involving growth-forms of total plants as well as vertical layers of woody plants. The results showed that, except for the fourth layer of woody plants, the vertical structure of the four forests exhibited significant difference in terms of their species richness, species diversity and species evenness, suggesting that α species diversity index provided more detailed information and was a better index reflecting the regeneration processes in this region. It was recommended that silvicultural management of artificially regenerated forests should be strengthened to conserve the biodiversity of the forests, optimize forest structure and, in the long run, promote sustainable development of forest ecosystems. | ZHANG Qiaoying ZHANG Yunchun Eshetu Yirdaw LUO Peng YI Shaoliang Wu Ning | 2006 | Wuhan University Journal of Natural Sciences2006，11，4： | 1 |
| 20 | Degradation of tetracycline and sulfadiazine during continuous thermophilic composting of pig manure and sawdust显示文摘 | Ammaiyappan Selvam Zhenyong Zhao Yunchun Li Yumei Chen Kelvin Leung Jonathan Wong | 2013 | Environmental Technology2013，，16： | 1 |
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