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| 1 | Three-dimensional bioprinted glioblastoma microenvironments model cellular dependencies and immune interactions显示文摘Brain tumors are dynamic complex ecosystems with multiple cell types.To model the brain tumor microenvironment in a reproducible and scalable system,we developed a rapid three-dimensional(3D)bioprinting method to construct clinically relevant biomimetic tissue models.In recurrent glioblastoma,macrophages/microglia prominently contribute to the tumor mass.To parse the function of macrophages in 3D,we compared the growth of glioblastoma stem cells(GSCs)alone or with astrocytes and neural precursor cells in a hyaluronic acid-rich hydrogel,with or without macrophage.Bioprinted constructs integrating macrophage recapitulate patient-derived transcriptional profiles predictive of patient survival,maintenance of stemness,invasion,and drug resistance.Whole-genome CRISPR screening with bioprinted complex systems identified unique molecular dependencies in GSCs,relative to sphere culture.Multicellular bioprinted models serve as a scalable and physiologic platform to interrogate drug sensitivity,cellular crosstalk,invasion,context-specific functional dependencies,as well as immunologic interactions in a species-matched neural environment. | Min Tang Qi Xie Ryan C.Gimple Zheng Zhong Trevor Tam Jing Tian Reilly L.Kidwell Qiulian Wu Briana C.Prager Zhixin Qiu Aaron Yu Zhe Zhu Pinar Mesci Hui Jing Jacob Schimelman Pengrui Wang Derrick Lee Michael H.Lorenzini Deobrat Dixit Linjie Zhao Shruti B.hargava Tyler E.Miller Xueyi Wan Jing Tang Bingjie Sun Benjamin F.Cravatt Alysson R.Muotri Shaochen Chen Jeremy N.Rich | 2020 | Cell Research2020，30，10： | 6 |
| 2 | PDGFRαreporter activity identifies periosteal progenitor cells critical for bone formation and fracture repair显示文摘The outer coverings of the skeleton,which is also known as the periosteum,are arranged in concentric layers and act as a reservoir for tissue-specific bone progenitors.The cellular heterogeneity within this tissue depot is being increasingly recognized.Here,inducible PDGFRαreporter animals were found to mark a population of cells within the periosteum that act as a stem cell reservoir for periosteal appositional bone formation and fracture repair.During these processes,PDGFRαreporter^(+)progenitors give rise to Nestin+periosteal cells before becoming osteoblasts and osteocytes.The diphtheria toxin-mediated ablation of PDGFRαreporter^(+)cells led to deficits in cortical bone formation during homeostasis and a diminutive hard callus during fracture repair.After ossicle transplantation,both mouse PDGFRαreporter^(+)periosteal cells and human Pdgfrα+periosteal progenitors expand,ossify,and recruit marrow to a greater extent than their counterpart periosteal cells,whereas PDGFRαreporter^(−)periosteal cells exhibit a predisposition to chondrogenesis in vitro.Total RNA sequencing identified enrichment of the secreted factors Fermt3 and Ptpn6 within PDGFRαreporter^(+)periosteal cells,which partly underlie the osteoblastogenic features of this cell population. | Jiajia Xu Yiyun Wang Zhu Li Ye Tian Zhao Li Amy Lu Ching-Yun Hsu Stefano Negri Cammy Tang Robert J.Tower Carol Morris Aaron W.James | 2022 | Bone Research2022，10，1： | 2 |
| 3 | The United Kingdom public health re- sponse to an imported laboratory confirmed case of a no- vel coronavirus in September 2012显示文摘 | Pebody R G Chand M A Thomas H L Green H K Boddington N L Carvalho C Brown C S Anderson S R Rooney C Crawley-Boevey E Irwin D J Aarons E Tong C Newsholme W Price N Langrish C Tucker D Zhao H Phin N Crofts J Bermingham A Gil- gunn-Jones E Brown K E Evans B Catchpole M Watson J M | 2012 | Euro Surveill2012，17，20： | 1 |
| 4 | Nanomaterial scaffolds for stem cell proliferation and differentiation in tissue engineering显示文摘 | Zhao Chunyan Tan Aaron Pastorin G | 2013 | Biotechnology Advances2013，31，5： | 1 |
| 5 | The proline synthesis enzyme P5CS forms cytoophidia in Drosophila显示文摘Compartmentation of enzymes via filamentation has arisen as a mechanism for the regulation of metabolism.In 2010,three groups independently reported that CTP synthase(CTPS)can assemble into a filamentous structure termed the cytoophidium.In searching for CTPS-interacting proteins,here we perform a yeast two-hybrid screening of Drosophila proteins and identify a putative CTPS-interacting protein,△~1-pyrroline-5-carboxylate synthase(P5CS).Using the Drosophila follicle cell as the in vivo model,we confirm that P5CS forms cytoophidia,which are associated with CTPS cytoophidia.Overexpression of P5CS increases the length of CTPS cytoophidia.Conversely,filamentation of CTPS affects the morphology of P5CS cytoophid ia.Finally,in vitro analyses confirm the filament-fo rming property of P5CS.Our work links CTPS with P5CS,two enzymes involved in the rate-limiting steps in pyrimidine and proline biosynthesis,respectively. | Bo Zhang Omür Y.Tastan Xian Zhou Chen-Jun Guo Xuyang Liu Aaron Thind Huan-Huan Hu Suwen Zhao Ji-Long Liu | 2020 | Journal of Genetics and Genomics2020，47，3： | 1 |
| 6 | Diagnostic Criteria and Yield of Real-Time Transcranial Doppler Monitoring of Intra-Arterial Reperfusion Procedures显示文摘 | Marta Rubiera Luis Cava Georgios Tsivgoulis Damon E. Patterson Limin Zhao Yi Zhang Aaron M. Anderson Alice Robinson Mark R. Harrigan Edward Underwood Joseph Horton Andrei V. Alexandrov | 2010 | Stroke2010，，4： | 1 |
| 7 | Nat Methods:利用CRISPR-Cas9组合筛选发现癌症弱点显示文摘导致癌症的基因突变也会削弱癌细胞,从而使得人们有机会开发选择性地杀死它们同时不影响正常细胞的药物。这一概念被称作”合成致死性(synthetic lethality)”,这是因为这些药物仅对发生突变的(或者说合成的)细胞是致命性的。在一项新的研究中。来自美国加州大学圣地亚哥分校、加州大学旧金山分校、斯坦福大学和癌细胞图谱项目(Cancer Cell Map Initiative)的研究人员开发出一种新的方法来寻找合成致死性的基因组合。 | John Paul Shen, Ana Bojorquez-Gomez, Katherine Licon, Kristin Klepper, Daniel Pekin, Alex N Beckett, Kyle Salinas Sanchez, Jason F Kreisberg, Trey Ideker John Paul Shen, Trey Ideker, Prashant Mali John Paul Shen, Dongxin Zhao, Dan Du, Assen Roguev, Jason F Kreisberg, Nevan Krogan, Lei Qi, Trey Ideker, Prashant Mali Dongxin Zhao, Chih-Chung Kuo, Prashant Mali Roman Sasik, Amanda Birmingham, Aaron N Chang, Trey Ideker Jens Luebeck, Alex Thomas Alex Thomas, Chih-Chung Kuo, Nathan E Lewis Dan Du Assen Roguev, Nevan Krogan Nathan E Lewis Lei Qi | 2017 | 现代生物医学进展2017，17，17： | 1 |
| 8 | microRNA and inflammatory gene expression as prognostic marker for overall survival in esophageal squamous cell carcinoma显示文摘 | Yiqiang Zhao Aaron J. Schetter Geoffrey B. Yang Giang Nguyen Ewy A. Mathé Peng Li Hong Cai Lei Yu Fangfang Liu Dong Hang Haijun Yang Xin Wei Wang Yang Ke Curtis C. Harris | 2012 | Cancer2012，，12： | 1 |
| 9 | microRNA and inflammatory gene expression as prognostic marker for overall survival in esophageal squamous cell carcinoma显示文摘 | Yiqiang Zhao Aaron J. Schetter Geoffrey B. Yang Giang Nguyen Ewy A. Mathé Peng Li Hong Cai Lei Yu Fangfang Liu Dong Hang Haijun Yang Xin Wei Wang Yang Ke Curtis C. Harris | 2012 | Int. J. Cancer2012，，12： | 1 |
| 10 | Neuron-to-vessel signaling is a required feature of aberrant stem cell commitment after soft tissue trauma显示文摘The functional interdependence of nerves and blood vessels is a well-established concept during tissue morphogenesis, yet the role of neurovascular coupling in proper and aberrant tissue repair is an emerging field of interest. Here, we sought to define the regulatory relationship of peripheral nerves on vasculature in a severe extremity trauma model in mice, which results in aberrant cell fate and heterotopic ossification(HO). First, a high spatial degree of neurovascular congruency was observed to exist within extremity injury associated heterotopic ossification. Vascular and perivascular cells demonstrate characteristic responses to injury,as assessed by single cell RNA sequencing. This vascular response to injury was blunted in neurectomized mice, including a decrease in endothelial proliferation and type H vessel formation, and a downregulation of key transcriptional networks associated with angiogenesis. Independent mechanisms to chemically or genetically inhibit axonal ingrowth led to similar deficits in HO site angiogenesis, a reduction in type H vessels, and heterotopic bone formation. Finally, a combination of single cell transcriptomic approaches within the dorsal root ganglia identified key neural-derived angiogenic paracrine factors that may mediate neuron-to-vascular signaling in HO. These data provide further understanding of nerve-to-vessel crosstalk in traumatized soft tissues, which may reflect a key determinant of mesenchymal progenitor cell fate after injury. | Qizhi Qin Mario Gomez-Salazar Masnsen Cherief Chase A.Pagani Seungyong Lee Charles Hwang Robert J.Tower Sharon Onggo Yuxiao Sun Abhinav Piplani Zhao Li Sowmya Ramesh Thomas L.Clemens Benjamin Levi Aaron W.James | 2022 | Bone Research2022，10，3： | 1 |
| 11 | VEGF-B prevents excessive angiogenesis by inhibiting FGF2/FGFR1 pathway显示文摘Although VEGF-B was discovered as a VEGF-A homolog a long time ago,the angiogenic effect of VEGF-B remains poorly understood with limited and diverse findings from different groups.Notwithstanding,drugs that inhibit VEGF-B together with other VEGF family members are being used to treat patients with various neovascular diseases.It is therefore critical to have a better understanding of the angiogenic effect of VEGF-B and the underlying mechanisms.Using comprehensive in vitro and in vivo methods and models,we reveal here for the first time an unexpected and surprising function of VEGF-B as an endogenous inhibitor of angiogenesis by inhibiting the FGF2/FGFR1 pathway when the latter is abundantly expressed.Mechanistically,we unveil that VEGF-B binds to FGFR1,induces FGFR1/VEGFR1 complex formation,and suppresses FGF2-induced Erk activation,and inhibits FGF2-driven angiogenesis and tumor growth.Our work uncovers a previously unrecognized novel function of VEGF-B in tethering the FGF2/FGFR1 pathway.Given the anti-angiogenic nature of VEGF-B under conditions of high FGF2/FGFR1 levels,caution is warranted when modulating VEGF-B activity to treat neovascular diseases. | Chunsik Lee Rongyuan Chen Guangli Sun Xialin Liu Xianchai Lin Chang He Liying Xing Lixian Liu Lasse DJensen Anil Kumar Harald FLanger Xiangrong Ren Jianing Zhang Lijuan Huang Xiangke Yin JongKyong Kim Juanhua Zhu Guanqun Huang Jiani Li Weiwei Lu Wei Chen Juanxi Liu Jiaxin Hu Qihang Sun Weisi Lu Lekun Fang Shasha Wang Haiqing Kuang Yihan Zhang Geng Tian Jia Mi Bi-Ang Kang Masashi Narazaki Aaron Prodeus Luc Schoonjans David MOrnitz Jean Gariepy Guy Eelen Mieke Dewerchin Yunlong Yang Jing-Song Ou Antonio Mora Jin Yao Chen Zhao Yizhi Liu Peter Carmeliet Yihai Cao Xuri Li | 2023 | Signal Transduction and Targeted Therapy2023，8，9： | 0 |
| 12 | Tppp3~+synovial/tendon sheath progenitor cells contribute to heterotopic bone after trauma显示文摘Heterotopic ossification(HO)is a pathological process resulting in aberrant bone formation and often involves synovial lined tissues.During this process,mesenchymal progenitor cells undergo endochondral ossification.Nonetheless,the specific cell phenotypes and mechanisms driving this process are not well understood,in part due to the high degree of heterogeneity of the progenitor cells involved.Here,using a combination of lineage tracing and single-cell RNA sequencing(sc RNA-seq),we investigated the extent to which synovial/tendon sheath progenitor cells contribute to heterotopic bone formation.For this purpose,Tppp3(tubulin polymerization-promoting protein family member 3)-inducible reporter mice were used in combination with either Scx(Scleraxis)or Pdgfra(platelet derived growth factor receptor alpha)reporter mice.Both tendon injury-and arthroplasty-induced mouse experimental HO models were utilized.Sc RNA-seq of tendon-associated traumatic HO suggested that Tppp3 is an early progenitor cell marker for either tendon or osteochondral cells.Upon HO induction,Tppp3 reporter^(+)cells expanded in number and partially contributed to cartilage and bone formation in either tendon-or joint-associated HO.In double reporter animals,both Pdgfra^(+)Tppp3^(+)and Pdgfra^(+)Tppp3^(-) progenitor cells gave rise to HO-associated cartilage.Finally,analysis of human samples showed a substantial population of TPPP3^(-) expressing cells overlapping with osteogenic markers in areas of heterotopic bone.Overall,these data demonstrate that synovial/tendon sheath progenitor cells undergo aberrant osteochondral differentiation and contribute to HO after trauma. | Ji-Hye Yea Mario Gomez-Salazar Sharon Onggo Zhao Li Neelima Thottappillil Masnsen Cherief Stefano Negri Xin Xing Qizhi Qin Robert Joel Tower Chen-Ming Fan Benjamin Levi Aaron W.James | 2023 | Bone Research2023，11，3： | 0 |
| 13 | 新型HfO_2栅介质中的频散效应及参数提取方法显示文摘本文研究了原子层化学气相淀积ALCVD(atom layer chemical vapor deposition)方法淀积的HfO2/SiO2/p-SiMOS电容的电特性.高频时,积累电容出现了频率色散现象.针对双频C-V法测量超薄HfO2/SiO2堆栈栅MOS电容中制备工艺和测量设备引入的寄生效应,给出了改进的等效电路模型,消除了频率色散.研究发现,高k介质中存在的缺陷和SiO2/Si处的界面态,使高频C-V特性发生漂移.对禁带中界面态的分布进行归纳,得到C-V曲线形变的规律.研究了形变的C-V曲线与理想C-V特性的偏离,给出了界面态电荷密度的分布,得到了相对于实测C-V曲线的矫正线.通过比较理想C-V曲线和矫正线,提取了平带电压、栅氧化层电荷、SiO2/Si界面的界面态密度等典型的电学参数. | 刘红侠 匡潜玮 栾苏珍 ZHAO Aaron TALLAVARJULA Sai | 2010 | 中国科学:信息科学2010，40，6： | 0 |
| 14 | Electrolyte/Structure‑Dependent Cocktail Mediation Enabling High‑Rate/Low‑Plateau Metal Sulfide Anodes for Sodium Storage显示文摘As promising anodes for sodium-ion batteries,metal sulfides ubiquitously suffer from low-rate and high-plateau issues,greatly hindering their application in full-cells.Herein,exemplifying carbon nanotubes(CNTs)-stringed metal sulfides superstructure(CSC)assembled by nano-dispersed SnS_(2) and CoS_(2) phases,cocktail mediation effect similar to that of high-entropy materials is initially studied in ether-based electrolyte to solve the challenges.The high nano-dispersity of metal sulfides in CSC anode underlies the cocktail-like mediation effect,enabling the circumvention of intrinsic drawbacks of different metal sulfides.By utilizing ether-based electrolyte,the reversibility of metal sulfides is greatly improved,sustaining a long-life effectivity of cocktail-like mediation.As such,CSC effectively overcomes low-rate flaw of SnS_(2) and highplateau demerit of CoS_(2),simultaneously realizes a high rate and a low plateau.In half-cells,CSC delivers an ultrahigh-rate capability of 327.6 mAh g^(−1) anode at 20 A g^(−1),far outperforming those of monometallic sulfides(SnS_(2),CoS_(2))and their mixtures.Compared with CoS_(2) phase and SnS_(2)/CoS_(2) mixture,CSC shows remarkably lowered average charge voltage up to ca.0.62 V.As-assembled CSC//Na1.5VPO4.8F0.7 full-cell shows a good rate capability(0.05~1.0 A g^(−1),120.3 mAh g^(−1) electrode at 0.05 A g^(−1))and a high average discharge voltage up to 2.57 V,comparable to full-cells with alloy-type anodes.Kinetics analysis verifies that the cocktail-like mediation effect largely boosts the charge transfer and ionic diffusion in CSC,compared with single phase and mixed phases.Further mechanism study reveals that alternative and complementary electrochemical processes between nano-dispersed SnS_(2) and CoS_(2) phases are responsible for the lowered charge voltage of CSC.This electrolyte/structure-dependent cocktail-like mediation effect effectively enhances the practicability of metal sulfide anodes,which will boost the development of high-rate/-voltage sodium-ion full batteries. | Yongchao Tang Yue Wei Anthony F.Hollenkamp Mustafa Musameh Aaron Seeber Tao Jin Xin Pan Han Zhang Yanan Hou Zongbin Zhao Xiaojuan Hao Jieshan Qiu Chunyi Zhi | 2021 | Nano-Micro Letters2021，13，11： | 0 |
| 15 | Modeling Common-Mode Current Due to Asymmetric Aging of Machine Winding Insulation显示文摘Machine stator winding insulation degradation is one of the main results of machine aging.It is non-negligible once this degradation process becomes asymmetric between phases.The traditional way to determine the insulation state of health is a partial discharge test.However,this method requires the system offline,which causes production loss and extra administrative burden.This paper presents an idea for better characterizing the insulation machine’s state of health using common-mode(CM)behavior in the machine-drive system.With the help of circuit decomposition methods and modeling tools,the CM quantities due to asymmetric aging show a unique characteristic that distinguishes itself from other differential-mode(DM)quantities in the equivalent circuit.It is shown effective to represent the asymmetric aging effect from the detection of system leakage current.This paper provides an analytical method to quantify this characteristic from mathematical approaches,and a proper approximation has been made on the CM equivalent model(CEM)such that the CM behavior is accurately characterized.The proposed method will serve the purpose of predicting machine abnormal behavior using the simple RLC circuit.Researchers can adapt this method to quantify and characterize the machine insulation state of health(SOH). | Jin Zhao Aaron D.Brovont | 2023 | Complex System Modeling and Simulation2023，3，2： | 0 |
| 16 | Frequency dispersion effect and parameters extraction method for novel HfO_2 as gate dielectric显示文摘The electric characteristic of MOS capacitor with HfO_2/SiO_2/p-Si grown by ALCVD (atom layer chemical vapor deposition) is investigated. The C-V curves show that the accumulation capacitances take on the frequency dispersion at high frequency. For MOS capacitor with ultra thin HfO_2/SiO_2 gate stack, different fabrication processes and measurement equipment will cause parasitic effect. Here an equivalent circuit model that can eliminate the frequency dispersion effect is proposed. The C-V characteristics curve at high frequency shows some distortion because of the bulk defects and the interface states. This paper discusses the distortion of the high frequency MOS C-V characteristic curve. A data processing method is advanced and interface trap density distribution in the band gap is presented. By comparing the ideal C-V curve with the experimental C-V curve, the typical electrical parameters of MOS capacitor are extracted, including the shift of flat-band voltage, the oxide charges and the density of interface traps at the SiO_2/Si interface. | LIU HongXia KUANG QianWei LUAN SuZhen ZHAO Aaron TALLAVARJULA Sai | 2010 | Science China(Information Sciences)2010，53，4： | 0 |
| 17 | Fibromodulin reduces scar formation in adult cutaneouswounds by eliciting a fetal-like phenotype显示文摘Blocking transforming growth factor(TGF)β1 signal transduction has been a central strategy for scar reduction;however,this approach appears to be minimally effective.Here,we show that fibromodulin(FMOD),a 59-kD small leucine-rich proteoglycan critical for normal collagen fibrillogenesis,significantly reduces scar formation while simultaneously increasing scar strength in both adult rodent models and porcine wounds,which simulate human cutaneous scar repair.Mechanistically,FMOD uncouples promigration/contraction cellular signals from pro-fibrotic signaling by selectively enhancing SMAD3-mediated signal transduction,while reducing AP-1-mediated TGFβ1 auto-induction and fibrotic extracellular matrix accumulation.Consequently,FMOD accelerates TGFβ1-responsive adult fibroblast migration,myofibroblast conversion,and function.Furthermore,our findings strongly indicate that,by delicately orchestrating TGFβ1 activities rather than indiscriminately blocking TGFβ1,FMOD elicits fetal-like cellular and molecular phenotypes in adult dermal fibroblasts in vitro and adult cutaneous wounds in vivo,which is a unique response of living system undescribed previously.Taken together,this study illuminates the signal modulating activities of FMOD beyond its structural support functions,and highlights the potential for FMOD-based therapies to be used in cutaneous wound repair. | Zhong Zheng Aaron W James Chenshuang Li Wenlu Jiang Joyce Z Wang Grace X Chang Kevin S Lee Feng Chen Emily A Berthiaume Yao Chen Hsin Chuan Pan Eric C Chen Weiming Li Zhihe Zhao Xinli Zhang Kang Ting Chia Soo | 2017 | Signal Transduction and Targeted Therapy2017，2，1： | 0 |
| 18 | 拍卖行化身多元艺术空间——嘉德艺术中心显示文摘嘉德艺术中心紧邻紫禁城,作为一座全新的综合文化机构,将北京的古城韵味与现代大都会的蓬勃生机以及多元艺术融为一体.嘉德艺术中心是世界上第一个量身定制的拍卖行总部,在北京的中心创建了一个全新的多元艺术机构类型.建筑提供博物馆硬件质量的展示空间和最先进的艺术品保护设施,同时也是一个灵活丰富的文化空间,与餐馆、酒店、公共交通基础设施结合在一起. | Marcel Holmberg Sun Ke Emily Liang Cecilia Lei Yingda Ahrens Mark Biemans Kim Bjarke Catarina Canas Alicia Casals Michael Cavander Jeffrey Cheng Patrick Conway Dyno Du Nicolaz Frez Brian Fung Daniel Hawkins Xinran Ji Philipp Kramer Emeline Laurencon Yuyang Lin Mavis Liu Wymen Lo Max Ma Rafael Merino John Murphey Marcin Olszowski Kevin Ou Kevin Park Yanyadech Phornphong Aaron Powers Klementina Savickaite Jim Shi Chiara Storino Joseph Tang Yang Tao Elena Yang Quentin Yiu Francis Young Lei Yu Danny Zhang Bruno Zhao Weiwei Zhang Yi Zhu Catarina Canas Brian Fung Paloma Hernaiz Marcel Holmberg Tait Kaplan Jaime Oliver Joseph Tang | 2018 | 设计家2018，，1： | 0 |
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