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| 1 | The role of furin cleavage site in SARS-CoV-2 spike protein-mediated membrane fusion in the presence or absence of trypsin显示文摘Dear Editor,The rapid spread of SARS-CoV-2(also known as 2019-nCoV and HCoV-191),a novel lineage B betacoronavirus(βCoV),has caused a global pandemic of coronavirus disease(COVID-19).It has been speculated that RRAR,a unique furin-like cleavage site(FCS)in the spike protein(S),which is absent in other lineage BβCoVs,such as SARS-CoV,is responsible for its high infectivity and transmissibility. | Shuai Xia Qiaoshuai Lan Shan Su Xinling Wang Wei Xu Zezhong Liu Yun Zhu Qian Wang Lu Lu Shibo Jiang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 8 |
| 2 | RBD-Fc-based COVID-19 vaccine candidate induces highly potent SARS-CoV-2 neutralizing antibody response显示文摘The pandemic of coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has posed serious threats to global health and economy,thus calling for the development of safe and effective vaccines.The receptorbinding domain(RBD)in the spike protein of SARS-CoV-2 is responsible for its binding to angiotensin-converting enzyme 2(ACE2)receptor.It contains multiple dominant neutralizing epitopes and serves as an important antigen for the development of COVID-19 vaccines.Here,we showed that immunization of mice with a candidate subunit vaccine consisting of SARS-CoV-2 RBD and Fc fragment of human IgG,as an immunopotentiator,elicited high titer of RBD-specific antibodies with robust neutralizing activity against both pseudotyped and live SARS-CoV-2 infections.The mouse antisera could also effectively neutralize infection by pseudotyped SARS-CoV-2 with several natural mutations in RBD and the IgG extracted from the mouse antisera could also show neutralization against pseudotyped SARS-CoV and SARS-related coronavirus(SARSr-CoV).Vaccination of human ACE2 transgenic mice with RBD-Fc could effectively protect mice from the SARS-CoV-2 challenge.These results suggest that SARS-CoV-2 RBD-Fc has good potential to be further developed as an effective and broad-spectrum vaccine to prevent infection of the current SARS-CoV-2 and its mutants,as well as future emerging SARSr-CoVs and re-emerging SARS-CoV. | Zezhong Liu Wei Xu Shuai Xia Chenjian Gu Xinling Wang Qian Wang Jie Zhou Yanling Wu Xia Cai Di Qu Tianlei Ying Youhua Xie Lu Lu Zhenghong Yuan Shibo Jiang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 7 |
| 3 | A novel STING agonist-adjuvanted pan-sarbecovirus vaccine elicits potent and durable neutralizing antibody and T cell responses in mice,rabbits and NHPs显示文摘The emergence of SARS-CoV-2 variants and potentially other highly pathogenic sarbecoviruses in the future highlights the need for pan-sarbecovirus vaccines.Here,we discovered a new STING agonist,CF501,and found that CF501-adjuvanted RBD-FC vaccine(CF501/RBD-FC)elicited significantly stronger neutralizing antibody(nAb)and T cell responses than Alum-and cGAMP-adjuvanted RBD-FC in mice.Vaccination of rabbits and rhesus macaques(nonhuman primates,NHPs)with CF501/RBD-FC elicited exceptionally potent nAb responses against SARS-CoV-2 and its nine variants and 41 S-mutants,SARS-CoV and bat SARSr-CoVs.CF501/RBD-FC-immunized hACE2-transgenic mice were almost completely protected against SARS-CoV-2 challenge,even 6 months after the initial immunization.NHPs immunized with a single dose of CF501/RBD-FC produced high titers of nAbs.The immunized macaques also exhibited durable humoral and cellular immune responses and showed remarkably reduced viral load in the upper and lower airways upon SARS-CoV-2 challenge even at 108 days post the final immunization.Thus,CF501/RBD-Fc can be further developed as a novel pan-sarbecovirus vaccine to combat current and future outbreaks of sarbecovirus diseases. | Zezhong Liu Jie Zhou Wei Xu Wei Deng Yanqun Wang Meiyu Wang Qian Wang Ming Hsieh Jingming Dong Xinling Wang Weijin Huang Lixiao Xing Miaoling He Chunlin Tao Youhua Xie Yilong Zhang Youchun Wang Jincun Zhao Zhenghong Yuan Chuan Qin Shibo Jiang Lu Lu | 2022 | Cell Research2022，32，3： | 5 |
| 4 | A highly potent and stable pan-coronavirus fusion inhibitor as a candidate prophylactic and therapeutic for COVID-19 and other coronavirus diseases显示文摘The development of broad-spectrum antivirals against human coronaviruses(HCoVs)is critical to combat the current coronavirus disease 2019(COVID-19)pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and its variants,as well as future outbreaks of emerging CoVs.We have previously identified a polyethylene glycol-conjugated(PEGylated)lipopeptide,EK1C4,with potent pan-CoV fusion inhibitory activity.However,PEG linkers in peptide or protein drugs may reduce stability or induce anti-PEG antibodies in vivo.Therefore,we herein report the design and synthesis of a series of dePEGylated lipopeptide-based pan-CoV fusion inhibitors featuring the replacement of the PEG linker with amino acids in the heptad repeat 2 C-terminal fragment(HR2-CF)of HCoV-OC43.Among these lipopeptides,EKL1C showed the most potent inhibitory activity against infection by SARS-CoV-2 and its spike(S)mutants,as well as other HCoVs and some bat SARS-related coronaviruses(SARSr-CoVs)tested.The dePEGylated lipopeptide EKL1C exhibited significantly stronger resistance to proteolytic enzymes,better metabolic stability in mouse serum,higher thermostability than the PEGylated lipopeptide EK1C4,suggesting that EKL1C could be further developed as a candidate prophylactic and therapeutic for COVID-19 and other coronavirus diseases. | Jie Zhou Wei Xu Zezhong Liu Chao Wang Shuai Xia Qiaoshuai Lan Yanxing Cai Shan Su Jing Pu Lixiao Xing Youhua Xie Lu Lu Shibo Jiang Qian Wang | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 5 |
| 5 | Retraction Note to:SARS-CoV-2 infects T lymphocytes through its spike protein-mediated membrane fusion显示文摘The authors have retracted this article1.After the publication of this article,it came to the authors attention that in order to support the conclusions of the study,the authors should have used primary T cells instead of T-cell lines.In addition,there are concerns that the flow cytometry methodology applied here was flawed.These points resulted in the conclusions being considered invalid. | Xinling Wang Wei Xu Gaowei Hu Shuai Xia Zhiping Sun Zezhong Liu Youhua Xie Rong Zhang Shibo Jiang Lu Lu | 2020 | Cellular & Molecular Immunology2020，17，8： | 5 |
| 6 | A pan-sarbecovirus vaccine induces highly potent and durable neutralizing antibody responses in non-human primates against SARS-CoV-2 Omicron variant显示文摘Dear Editor,Since the outbreak of Coronavirus Disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection,numerous SARS-CoV-2 variants of concern(VOCs),such as Alpha(B.1.1.7),Beta(B.1.351),Gamma(P.1),and Delta(B.1.617.2),have emerged.Recently,the newly identified SARS-CoV-2 VOC,Omicron(B.1.1.529),has rapidly spread in many countries1 and is expected to replace Delta as the dominant variant circulating in the world.Compared to other VOCs,Omicron contains at least 32 mutations in spike(S)protein,including 15 mutations in the receptor-binding domain(RBD),and these mutations are known to confer resistance to neutralizing antibodies(nAbs)and sera of convalescent patients and people who have received COVID-19 vaccines.2–5 Therefore,vaccines able to induce potent and durable neutralizing antibodies against Omicron and other VOCs are urgently needed. | Zezhong Liu Jasper Fuk-Woo Chan Jie Zhou Meiyu Wang Qian Wang Guangxu Zhang Wei Xu Kenn Ka-Heng Chik Yilong Zhang Youchun Wang Kwok-Yung Yuen Lu Lu Shibo Jiang | 2022 | Cell Research2022，32，5： | 4 |
| 7 | Inefficiency of Sera from Mice Treated with Pseudotyped SARS-CoV to Neutralize 2019-nCoV Infection显示文摘Dear Editor,An outbreak of unusual pneumonia in Wuhan, China recently was caused by infection of a novel type of coronavirus. The virus and disease were denoted as 2019-nCoV and COVID-19, respectively, by the World Health Organization(WHO). Most recently, 2019-nCoV was renamed SARS-CoV-2 by Coronaviridae Study Group(CSG) of the International Committee on Taxonomy of Viruses(ICTV)(Gorbalenya et al. 2020)。 | Zezhong Liu Shuai Xia Xinling Wang Qiaoshuai Lan Wei Xu Qian Wang Shibo Jiang Lu Lu | 2020 | Virologica Sinica2020，35，3： | 3 |
| 8 | Repurposing of a clinically used anti-HPV agent to prevent and treat SARS-CoV-2 infection as an intranasal formulation显示文摘Dear Editor,Fan et al.have shown that whey protein,such as that found in human,bovine,and goat milk,could effectively inhibit SARS-CoV-2 infection,but they did not identify the components in whey protein having this activity.1 Since p-lactoglobulin(P-LG)is the main component of whey protein,we herein tested the anti-SARS-CoV-2 activity of p-LG and 3-hydroxyphthalic anhydride(3HP)-modified P-LG(3HP-P-LG),an active component in anti-human papillomavirus(HPV)biological dressing(JB01-BD)that has been used in clinics since 2013 to block cervical infection of HPV. | Chen Hua Qinhai Ma Yun Zhu Shuai Xia Zezhong Liu Lin Li Lu Lu Nanshan Zhong Shuwen Liu Zifeng Yang Shibo Jiang | 2021 | Signal Transduction and Targeted Therapy2021，6，9： | 1 |
| 9 | Preparation of Titanium-based Polyoxometalatxiue-ionic Liquid Catalysts and Application in the Synthesis of Degradable Polyester显示文摘Three kinds of Keggin-type titanium-based polyoxometalate-ionic liquid catalysts were prepared,and the chemical structure,crystal structure and thermal stability of the three catalysts were analyzed and tested by infrared spectrometer,ultraviolet-visible spectrometer,X-ray diffractometer,and thermogravimetric analyzer,respectively.Afterwards,their performance in catalyzing the coupling reaction of ethylene carbonate(EC)and dimethyl succinate(DMSu)to synthesize polyethylene succinate(PES)was studied,and the effects of different metals on the catalytic performance and the mechanism of action were discussed.The optimal process parameters for the coupling reaction were explored.Finally,the chemical structure of PES was characterized by infrared spectrometer.The results show that under the same process parameters,the polyoxometalate-ionic liquid catalyst Bmim_(5)[Ti(H_(2)O)TiMo_(11)O_(39)]had the best catalytic effect.Under the optimal process parameters,Bmim_(5)[Ti(H_(2)O)TiMo_(11)O_(39)]had obvious inhibitory effect on side reactions,and the yield and selectivity of PES prepolymer were up to 72.23%and 88.64%. | Ran CHEN Changyuan YU Liying GUO Kun LIU Xiudan ZHAO Zezhong JIANG | 2022 | Meteorological and Environmental Research2022，13，4： | 1 |
| 10 | Double insult: flu bug enhances SARS-CoV-2 infectivity显示文摘The continuing COVID-19 pandemic and emergence of SARS-CoV-2 variants may cause this disease to transit into endemicity like seasonal flu.In a recent study,Bai et al.reported that preinfection of influenza A virus enhanced SARS-CoV-2 infectivity,calling for the development of a pan-flu-COVID-19 vaccine to combat infection of IAV,SARS-CoV-2,or their coinfection.The COVID-19 pandemic,caused by SARS-CoV-2,may become even more serious by the emergence of variants with altered transmissibility,pathogenesis,and virulence,or a combination,under evolutionary pressure. | Shan Su Zezhong Liu Shibo Jiang | 2021 | Cell Research2021，31，5： | 1 |
| 11 | The putative mechanism of lymphopenia in CoVID-19 patients显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and its variants have caused>5oo million confirmed cases and>6 million deaths.Apart from the common clinical manifestations,63% of admitted coronavirus disease 2019(COvID-19)patients had lymphopenia,increasingto 85% in patients with severe disease(Huang et al.,2020). | Xinling Wang Zezhong Liu Lu Lu Shibo Jiang | 2022 | Journal of Molecular Cell Biology2022，14，5： | 1 |
| 12 | An ultrapotent pan-β-coronavirus lineage B(β-CoV-B)neutralizing antibody locks the receptor-binding domain in closed conformation by targeting its conserved epitope显示文摘New threats posed by the emerging circulating variants of SARS-CoV-2 highlight the need to find conserved neutralizing epitopes for therapeutic antibodies and efficient vaccine design.Here,we identified a receptorbinding domain(RBD)-binding antibody,XG014,which potently neutralizesβ-coronavirus lineage B(β-CoV-B),including SARS-CoV-2,its circulating variants,SARSCoV and bat SARSr-CoV WIV1.Interestingly,antibody family members competing with XG014 binding show reduced levels of cross-reactivity and induce antibodydependent SARS-CoV-2 spike(S)protein-mediated cellcell fusion,suggesting a unique mode of recognition by XG014.Structural analyses reveal that XG014 recognizes a conserved epitope outside the ACE2 binding site and completely locks RBD in the non-functional“down”conformation,while its family member XG005 directly competes with ACE2 binding and position the RBD“up”.Single administration of XG014 is effective in protection against and therapy of SARS-CoV-2 infection in vivo.Our findings suggest the potential to develop XG014 as pan-β-CoV-B therapeutics and the importance of the XG014 conserved antigenic epitope for designing broadly protective vaccines againstβ-CoV-B and newly emerging SARS-CoV-2 variants of concern. | Zezhong Liu Wei Xu Zhenguo Chen Wangjun Fu Wuqiang Zhan Yidan Gao Jie Zhou Yunjiao Zhou Jianbo Wu Qian Wang Xiang Zhang Aihua Hao Wei Wu Qianqian Zhang Yaming Li Kaiyue Fan Ruihong Chen Qiaochu Jiang Christian TMayer Till Schoofs Youhua Xie Shibo Jiang Yumei Wen Zhenghong Yuan Kang Wang Lu Lu Lei Sun Qiao Wang | 2022 | Protein & Cell2022，13，9： | 1 |
| 13 | A Study of Influence of Temperature on Crack Propagation Behavior of Asphalt Concrete显示文摘 | XIE Tao JIANG Zezhong QIU Yanjun | 2009 | International Conference on Measuring Technology and Mechatronics Automation2009，，： | 1 |
| 14 | RETRACTED ARTICLE:SARS-CoV-2 infects T lymphocytes through its spike protein-mediated membrane fusion显示文摘The authors have retracted this article [1]. After the publication ofthis article, it came to the authors attention that in order tosupport the conclusions of the study, the authors should haveused primary T cells instead of T cell lines. Additionally, there areconcerns that the flow cytometry methodology applied here wasflawed. These points resulted in the conclusions being consideredinvalid. | Xinling Wang Wei Xu Gaowei Hu Shuai Xia Zhiping Sun Zezhong Liu Youhua Xie Rong Zhang Shibo Jiang Lu Lu | 2023 | Cellular & Molecular Immunology2023，20，5： | 0 |
| 15 | Up or down:where comes Omicron?显示文摘The current dominant SARS-CoV-2 variant Omicron BA.2 has spread globally within only two months.Recent works published in Cell Research and Science reveal the molecular basis of increased transmissibility of Omicron BA.1 and BA.2 and their possible mouse origin. | Zezhong Liu Lu Lu Shibo Jiang | 2022 | Cell Research2022，32，7： | 0 |
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