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| 1 | Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model显示文摘AIM To investigate gut microbial diversity and the interventional effect of Xiaoyaosan(XYS) in a rat model of functional dyspepsia(FD) with liver depression-spleen deficiency syndrome. METHODS The FD with liver depression-spleen deficiency syndrome rat model was established through classic chronic mild unpredictable stimulation every day. XYS group rats received XYS 1 h before the stimulation. The models were assessed by parameters including state ofthe rat, weight, sucrose test result and open-field test result. After 3 wk, the stools of rats were collected and genomic DNA was extracted. PCR products of the V4 region of 16 S rD NA were sequenced using a barcoded Illumina paired-end sequencing technique. The primary composition of the microbiome in the stool samples was determined and analyzed by cluster analysis.RESULTS Rat models were successfully established, per data from rat state, weight and open-field test. The microbiomes contained 20 phyla from all samples. Firmicutes, Bacteroidetes, Proteobacteria, Cyanobacteria and Tenericutes were the most abundant taxonomic groups. The relative abundance of Firmicutes, Proteobacteria and Cyanobacteria in the model group was higher than that in the normal group. On the contrary, the relative abundance of Bacteroidetes in the model group was lower than that in the normal group. Upon XYS treatment, the relative abundance of all dysregulated phyla was restored to levels similar to those observed in the normal group. Abundance clustering heat map of phyla corroborated the taxonomic distribution. CONCLUSION The microbiome relative abundance of FD rats with liver depression-spleen deficiency syndrome was significantly different from the normal cohort. XYS intervention may effectively adjust the gut dysbacteriosis in FD. | Juan-Juan Qiu Zhe Liu Peng Zhao Xue-Jun Wang Yu-Chun Li Hua Sui Lawrence Owusu Hui-Shu Guo Zheng-Xu Cai | 2017 | World Journal of Gastroenterology2017，23，5： | 23 |
| 2 | A linear ADRC-based robust high-dynamic double-loop servo system for aircraft electro-mechanical actuators显示文摘Compared with traditional hydraulic actuators, an Electro-Mechanical Actuator(EMA)is small in size and light in weight, so it has become more widely used. Aerodynamic load on aircraft control surface varies dramatically, and a change of flight environment leads to uncertainties of motor parameters. Therefore, high-dynamic response and strong anti-disturbance capability of an EMA are of great significance for aircraft rudder control and flight attitude adjustment. In order to improve dynamic response and disturbance rejection of an EMA and simplify control parameters tuning, a robust high-dynamic servo system based on Linear Active Disturbance Rejection Control(LADRC) is proposed for an EMA employing a Permanent Magnet Synchronous Motor(PMSM).Firstly, total disturbances of the EMA are analyzed, including parameter uncertainties, load variation, and static friction. A disturbance observer based on a reduced-order Extended State Observer(ESO) is designed to improve the anti-interference ability and dynamic performance. Secondly, the servo control architecture is simplified to a double-loop system, and a composite control of position and speed with acceleration feed-forward is presented to improve the EMA frequency bandwidth.Thirdly, the ideal model of the EMA is transformed into a simple cascade integral form with a disturbance observer, which makes it convenient to analyze and design the controller. Robustness performance comparisons are realized in frequency domain. Finally, simulation and experimental results have verified the effectiveness of the proposed strategy for EMAs. | Chunqiang LIU Guangzhao LUO Zhe CHEN Wencong TU Cai QIU | 2019 | Chinese Journal of Aeronautics2019，32，9： | 18 |
| 3 | Morphological and pathologic changes of experimental chronic atrophic gastritis (CAG)and the regulating mechanism of protein expression in rats显示文摘Objective: To study the pathologic change and molecular regulation in cell proliferation and apoptosis of gastric mucosa in rats with chronic atrophic gastritis (CAG), and evaluate the possible mechanisms. Methods: Rats were administered with 60% alcohol or 2% salicylate sodium, 20 mmol/L deoxycholate sodium and 0.1% ammonia water to establish chronic atrophic gastritis (CAG) models. The gastric specimens were prepared for microscopic view with hematoxylin and eosin (H-E) and alcian blue (A-B) stain. The number of infiltrated inflammatory cells, the thickness of the mucosa gland layer (μm) and the number of gastric glands were calculated. The damage of barrier in mucosa with erosion or ulceration, and the thickness of mucin were examined by scanned electron microscope (SEM). The levels of PGE2, EGF (epiderminal growth factor) and gastrin in the serum were measured with radioimmunoassay or ELISA method. The immunohistochemistry method was used to observe the number of G cells, the expression of protein of EGFR (EGF receptor), C-erbB-2, p53, p16 and bcl-2 in gastric tissue. Results: Under SEM observation, the gastric mucosa was diffused erosion or ulceration and the thickness of mucin was decreased. Com- pared with normal rats, the grade of inflammatory cell infiltration in CAG rats was elevated, whereas the thickness and number of gastric gland were significantly lower (P<0.05). Compared with normal level of (0.61±0.28) μg/L, EGF in CAG (2.24±0.83) μg/L was significantly higher (P<0.05). The levels of PGE2 and gastrin in serum were significantly lower in CAG rats than that in normal rats (P<0.05). Immunohistochemistry detection showed that the number of G cell in antrum was lower in CAG group (P<0.05). Immuno-stain showed EGFR protein expression in the basal and bilateral membrane, and the cytoplasma in atrophic gastric gland, while negative expression was observed in normal gastric epithelial cells. Positive staining of p53 and p16 protein was localized in the nucleus of epithelial cells. The former was higher positively expressed in atrophic gland, while the later was higher positively stained in normal gastric tissue. bcl-2 protein was positively stained in the cytoplasma in atrophic gastric gland, while very weakly stained in normal gastric tissue. Conclusion: The pathological findings in gastric gland accorded with the Houston diagnostic criteria of antrum-predominant CAG. CAG in rats was related with the damage of barrier in gastric mucosa and the misbalance of cell proliferation and apoptosis. There was high protein expression of oncogene, while inhibitor of sup- pressor gene in CAG rats indicated high trend of carcinogenesis. | WANG Liang-jing CHEN Shu-jie CHEN Zhe CAI Jian-ting SI Jian-min | 2006 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2006，7，8： | 13 |
| 4 | Dense ceramics with complex shape fabricated by 3D printing:A review显示文摘Three-dimensional(3D)printing technology is becoming a promising method for fabricating highly complex ceramics owing to the arbitrary design and the infinite combination of materials.Insufficient density is one of the main problems with 3D printed ceramics,but concentrated descriptions of making dense ceramics are scarce.This review specifically introduces the principles of the four 3D printing technologies and focuses on the parameters of each technology that affect the densification of 3D printed ceramics,such as the performance of raw materials and the interaction between energy and materials.The technical challenges and suggestions about how to achieve higher ceramic density are presented subsequently.The goal of the presented work is to comprehend the roles of critical parameters in the subsequent 3D printing process to prepare dense ceramics that can meet the practical applications. | Zhe Chen Xiaohong Sun Yunpeng Shang Kunzhou Xiong Zhongkai Xu Ruisong Guo Shu Cai Chunming Zheng | 2021 | Journal of Advanced Ceramics2021，10，2： | 10 |
| 5 | Review:Mathematically modeling fixed-bed adsorption in aqueous systems显示文摘Adsorption is one of the widely used processes in the chemical industry environmental application.As compared to mathematical models proposed to describe batch adsorption in terms of isotherm and kinetic behavior,insufficient models are available to describe and predict fixed-bed or column adsorption,though the latter one is the main option in practical application.The present review first provides a brief summary on basic concepts and mathematic models to describe the mass transfer and isotherm behavior of batch adsorption,which dominate the column adsorption behavior in nature.Afterwards,the widely used models developed to predict the breakthrough curve,i.e.,the general rate models,linear driving force(LDF) model,wave propagation theory model,constant pattern model,Clark model,Thomas model,Bohart-Adams model,Yoon-Nelson model,Wang model,Wolborska model,and modified dose-response model,are briefly introduced from the mechanism and mathematical viewpoint.Their basic characteristics,including the advantages and inherit shortcomings,are also discussed.This review could help those interested in column adsorption to reasonably choose or develop an accurate and convenient model for their study and practical application. | Zhe XU Jian-guo CAI Bing-cai PAN | 2013 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2013，14，3： | 10 |
| 6 | Hard X-ray Imager (HXI) onboard the ASO-S mission显示文摘Hard X-ray Imager(HXI)is one of the three scientific instruments onboard the Advanced Spacebased Solar Observatory(ASO-S)mission,which is proposed for the 25th solar maximum by the Chinese solar community.HXI is designed to investigate the non-thermal high-energy electrons accelerated in solar flares by providing images of solar flaring regions in the energy range from 30 keV to 200 keV.The imaging principle of HXI is based on spatially modulated Fourier synthesis and utilizes about 91 sets of bi-grid sub-collimators and corresponding LaBr3 detectors to obtain Fourier components with a spatial resolution of about 3 arcsec and a time resolution better than 0.5 s.An engineering prototype has been developed and tested to verify the feasibility of design.In this paper,we present background,instrument design and the development and test status of the prototype. | Zhe Zhang Deng-Yi Chen Jian Wu Jin Chang Yi-Ming Hu Yang Su Yan Zhang Jian-Ping Wang Yao-Ming Liang Tao Ma Jian-Hua Guo Ming-Sheng Cai Yong-Qiang Zhang Yong-Yi Huang Xiao-Yan Peng Zong-Bin Tang Xuan Zhao Hong-He Zhou Lian-Guo Wang Jing-Xing Song Miao Ma Guang-Zhou Xu Jian-Feng Yang Di Lu Ying-Hong He Jin-You Tao Xiao-Long Ma Bao-Gang Lv Yan-Ping Bai Cai-Xia Cao Yu Huang Wei-Qun Gan | 2019 | Research in Astronomy and Astrophysics2019，19，11： | 9 |
| 7 | Cross electro-nape-acupuncture ameliorates cerebral hemorrhageinduced brain damage by inhibiting necroptosis显示文摘BACKGROUND Receptor interacting protein kinase 1(RIPK1)-mediated cell death,including apoptosis and necroptosis,belongs to programmed cell death.It has been reported that RIPK1-mediated necroptosis exists in lesions of cerebral hemorrhage(CH).Electroacupuncture,a treatment derived from traditional Chinese medicine,could improve neurological impairment in patients with brain injury.AIM To investigate the protective role of cross electro-nape acupuncture(CENA)in CH,and clarify the potential mechanism.METHODS CH rat models were established,and CENA was applied to the experimental rats.Neurological functions and encephaledema were then measured.Necrotic cells in the brain of rats with CH were evaluated by propidium iodide staining.Necroptosis was assessed by immunofluorescence.Activation of the necroptosisrelated pathway was detected by western blot.Extraction of brain tissue,cerebrospinal fluid and serum samples was conducted to measure the expression and secretion of inflammatory cytokines by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay.RESULTS The necroptotic marker p-MLKL was detectable in the brains of rats with CH.Next,we found that CENA could ameliorate neurological functions in rat models of CH.Moreover,the upregulation of RIPK1-mediated necroptosis-related molecules in the brains of rats with CH were inhibited by CENA.Further investigation revealed that CENA partially blocked the interaction between RIPK1 and RIPK3.Finally,in vivo assays showed that CENA decreased the expression of the inflammatory cytokines tumor necrosis factor-α,interleukin-6 and interleukin-8 in CH rat models.CONCLUSION These findings revealed that CENA exerts a protective role in CH models by inhibiting RIPK1-mediated necroptosis. | Guo-Feng Cai Zhong-Ren Sun Zhe Zhuang Hai-Chun Zhou Shan Gao Kai Liu Li-Li Shang Kun-Ping Jia Xiu-Zhen Wang Hui Zhao Guo-Liang Cai Wen-Li Song Sheng-Nan Xu | 2020 | World Journal of Clinical Cases2020，8，10： | 9 |
| 8 | Diosgenin relieves goiter via the inhibition of thyrocyte proliferation in a mouse model of Graves' disease显示文摘 | Hu CAI Zhe WANG Hai-qing ZHANG Fu-rong WANG Chun-xiao YU Feng-xia ZHANG Ling GAO Jian ZHANG Jia-jun ZHAO | 2014 | Acta Pharmacologica Sinica2014，35，1： | 9 |
| 9 | NOD2 和 TLR2 ligands 由在遗传性过敏症的像皮炎的皮肤发炎与真皮的成纤维细胞交往触发 basophils 和嗜曙红血球的激活显示文摘有遗传性过敏症的皮炎(广告) 的病人的皮肤由葡萄球菌 aureus 为殖民有唯一的倾向(S。aureus ) ,它贡献发炎和广告的冷酷的预后。尽管位于 S 下面的机制。不清楚的、最近的研究在在 S 调整煽动性的回答为模式识别受体发现了一个枢轴的角色的广告遗体的导致 aureus 的恶化。aureus 感染。在现在的学习,我们使用了像广告的皮肤发炎的一个典型老鼠模型并且发现了那 S。联系 aureus 的核苷酸绑定 oligomerization 包含域的蛋白质 2 (NOD2 ) 并且像使用费的受体 2 (TLR2 ) ligands 加重了像广告的症状,它进一步被败坏由在里面 basophils 和嗜曙红血球的 vivo 扩大。随后的组织学的分析表明真皮的成纤维细胞在像广告的皮肤损害是弥漫的。有 basophils 和嗜曙红血球的人的真皮的成纤维细胞的合作文化在真皮的成纤维细胞上导致了对 NOD2/TLR2 ligands 和细胞间的粘附 molecule-1 的提高的表示的精力旺盛的 cytokine/chemokine 回答。Basophils 和嗜曙红血球为在合作文化的广告相关的 cytokine/chemokine 表示主要负责。直接细胞间的接触为在 basophils 和真皮的成纤维细胞之间的串音是必要的,当可溶的调停人是足够的调停时嗜曙红血球成纤维细胞相互作用。而且,细胞内部的 p38 激活 mitogen 的蛋白质 kinase,细胞外的调整信号的 kinase,和表明小径的原子 factor-kappa B 为在广告相关的发炎的 basophils,嗜曙红血球,和真皮的成纤维细胞的 NOD2/TLR2 调停 ligand 的激活是必要的。这研究通过天生的有免疫力的房间的激活提供广告的 NOD2/TLR2-mediated 恶化的证据因此使一条新奇机械学的小径由清楚些哪个 S。aureus 贡献广告的 pathophysiology。 | Delong Jiao Chun-Kwok Wong Huai-Na Qiu Jie Dong Zhe Cai Man Chu Kam-Lun Hon Miranda Sin-Man Tsang Christopher Wai-Kei Lam | 2016 | Cellular & Molecular Immunology2016，13，4： | 8 |
| 10 | Exosome-based small RNA delivery:Progress and prospects显示文摘RNA interfering(RNAi), mediated by small interfering RNAs and microRNAs, is currently one of the most promising tools of gene therapy. Small RNAs are capable of inducing specific post-transcriptional gene silencing, providing a potentially effective platform for the treatment of a wide array of diseases. However, similar to other nucleic acid-based drugs,the major hurdle of RNAi therapy is lack of efficient and non-immunogenic delivery vehicles. Currently, viruses, synthetic polymers, and lipid-based carriers are among the most widely studied vehicles for small RNA delivery. However, many drawbacks are reported to be associated with these delivery vehicles. There is a pressing need to replace them with more efficient and better-tolerated approaches. Exosomes secreted from the endocytic compartment of live cells, are a subtype of endogenous extracellular vesicles that transfer genetic and biochemical information among different cells, thus playing an important role in cellcell communication. Recently, accumulating attention has been focused on harnessing exosomes as nanaocarriers for small RNAs delivery. Due to their natural role in shuttling endogenous nucleic acid in our body, exosomes may exhibit higher delivery efficiency, lower immunogenicity, and better compatibility than existing foreign RNA carriers. Importantly,exosomes own intrinsic homing capacity that can guide small RNAs across natural membranous barriers. Moreover, such a capacity can be further improved by adding appropriate targeting moieties. In this manuscript, we briefly review the progress and challenges of RNAi therapy, and discuss the potential of exosomes' applications in small RNA delivery with focus on the most recent advances in exosome-based small RNA delivery for disease therapy. | Mei Lu Haonan Xing Zhe Xun Tianzhi Yang Pingtian Ding Cuifang Cai Dongkai Wang Xiaoyun Zhao | 2018 | Asian Journal of Pharmaceutical Sciences2018，13，1： | 8 |
| 11 | CircRNA-SORE mediates sorafenib resistance in hepatocellular carcinoma by stabilizing YBX1显示文摘Sorafenib is the first-line chemotherapeutic therapy for advanced hepatocellular carcinoma(HCC).However,sorafenib resistance significantly limits its therapeutic efficacy,and the mechanisms underlying resistance have not been fully clarified.Here we report that a circular RNA,circRNA-SORE(a circular RNA upregulated in sorafenib-resistant HCC cells),plays a significant role in sorafenib resistance in HCC.We found that circRNA-SORE is upregulated in sorafenib-resistant HCC cells and depletion of circRNA-SORE substantially increases the cell-killing ability of sorafenib.Further studies revealed that circRNA-SORE binds the master oncogenic protein YBX1 in the cytoplasm,which prevents YBX1 nuclear interaction with the E3 ubiquitin ligase PRP19 and thus blocks PRP19-mediated YBX1 degradation.Moreover,our in vitro and in vivo results suggest that circRNA-SORE is transported by exosomes to spread sorafenib resistance among HCC cells.Using different HCC mouse models,we demonstrated that silencing circRNA-SORE by injection of siRNA could substantially overcome sorafenib resistance.Our study provides a proof-of-concept demonstration for a potential strategy to overcome sorafenib resistance in HCC patients by targeting circRNA-SORE or YBX1. | Junjie Xu Lin Ji Yuelong Liang Zhe Wan Wei Zheng Xiaomin Song Kirill Gorshkov Qiming Sun Hui Lin Xueyong Zheng Jiang Chen Ren-an Jin Xiao Liang Xiujun Cai | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 12 | The predictive value of histological tumor regression grading (TRG) for therapeutic evaluation in locally advanced esophageal carcinoma treated with neoadjuvant chemotherapy显示文摘Response criteria remain controversial in therapeutic evaluation for locally advanced esophageal carcinoma treated with neoadjuvant chemotherapy.We aimed to identify the predictive value of tumor regression grading(TRG) in tumor response and prognosis.Fifty-two patients who underwent neoadjuvant chemotherapy followed by esophagectomy and radical 2-field lymphadenectomy between June 2007 and June 2011 were included in this study.All tissue specimens were reassessed according to the TRG scale.Potential prognostic factors,including clinicopathologic factors,were evaluated.Survival curves were generated by using the Kaplan-Meier method and compared with the log-rank test.Prognostic factors were determined with multivariate analysis by using the Cox regression model.Our results showed that of 52 cases,43(83%) were squamous cell carcinoma and 9(17%) were adenocarcinoma.TRG was correlated with pathologic T(P = 0.006) and N(P < 0.001) categories.Median overall survival for the entire cohort was 33 months.The 1-and 2-year overall survival rates were 71% and 44%,respectively.Univariate survival analysis results showed that favorable prognostic factors were histological subtype(P = 0.003),pathologic T category(P = 0.026),pathologic N category(P < 0.001),and TRG G0(P = 0.041).Multivariate analyses identified pathologic N category(P < 0.001) as a significant independent prognostic parameter.Our results indicate that histomorphologic TRG can be considered as an alternative option to predict the therapeutic efficacy and prognostic factor for patients with locally advanced esophageal carcinoma treated by neoadjuvant chemotherapy. | Kang Guo Ling Cai Yu Zhang Jian-Fei Zhu Tie-Hua Rong Peng Lin Chong-Li Hao Wu-Ping Wang Zhe Li Lan-Jun Zhang | 2012 | Chinese Journal of Cancer2012，31，8： | 3 |
| 13 | Oral microbiome and risk of malignant esophageal lesions in a high-risk area of China:A nested case-control study显示文摘Objective:We aimed to prospectively evaluate the association of oral microbiome with malignant esophageal lesions and its predictive potential as a biomarker of risk.Methods:We conducted a case-control study nested within a population-based cohort with up to 8 visits of oral swab collection for each subject over an 11-year period in a high-risk area for esophageal cancer in China.The oral microbiome was evaluated with 16 S ribosomal RNA(rRNA)gene sequencing in 428 pre-diagnostic oral specimens from 84 cases with esophageal lesions of severe squamous dysplasia and above(SDA)and 168 matched healthy controls.DESeq analysis was performed to identify taxa of differential abundance.Differential oral species together with subject characteristics were evaluated for their potential in predicting SDA risk by constructing conditional logistic regression models.Results:A total of 125 taxa including 37 named species showed significantly different abundance between SDA cases and controls(all P<0.05&false discovery rate-adjusted Q<0.10).A multivariate logistic model including 11 SDA lesion-related species and family history of esophageal cancer provided an area under the receiver operating characteristic curve(AUC)of 0.89(95%CI,0.84-0.93).Cross-validation and sensitivity analysis,excluding cases diagnosed within 1 year of collection of the baseline specimen and their matched controls,or restriction to screenendoscopic-detected or clinically diagnosed case-control triads,or using only bacterial data measured at the baseline,yielded AUCs>0.84.Conclusions:The oral microbiome may play an etiological and predictive role in esophageal cancer,and it holds promise as a non-invasive early warning biomarker for risk stratification for esophageal cancer screening programs. | Fangfang Liu Mengfei Liu Ying Liu Chuanhai Guo Yunlai Zhou Fenglei Li Ruiping Xu Zhen Liu Qiuju Deng Xiang Li Chaoting Zhang Yaqi Pan Tao Ning Xiao Dong Zhe Hu Huanyu Bao Hong Cai Isabel Dos Santos Silva Zhonghu He Yang Ke | 2020 | Chinese Journal of Cancer Research2020，32，6： | 3 |
| 14 | Charge recombination in dye-sensitized nanoporous TiO_(2)solar cell显示文摘DSSC has been a subject of intense study throughout the world. The efforts to investigate DSSC are mainly focused on how to increase light absorption, speed electron transport in circuit and reduce charge recombina-tion. In this article, the development of charge recombination in DSSC is discussed, and the investigating techniques, main paths, mechanism and main inhibiting methods of charge recombination in DSSC are also described. | ZHANG Zhe ZHOU Baoxue GE Weijie XIONG Bitao ZHENG Qing CAI Weimin | 2005 | Chinese Science Bulletin2005，50，21： | 3 |
| 15 | Genetic parameters and trends for production and reproduction traits of a Landrace herd in China显示文摘The objectives of this study were to estimate the genetic parameters and the breeding progress in a Landrace herd in China, and to predict the potential benefits by applying new breeding technology. Hereby, the performance records from a Landrace swine herd in China, composing over 33 000 pigs born between 2001 and 2013, were collected on six economically important traits, i.e., average daily gain between 30–100 kg(ADG), adjusted backfat thickness at 100 kg(BF), adjusted days to 30 kg(D30), adjusted days to 100 kg(D100), number born alive(NBA), and total number born(TNB). The genetic parameters were estimated by restricted maximum likelihood via DMU, and realized genetic trends were analyzed. Based on the real population structure and genetic parameters obtained from this herd, the potential genetic trends by applying genomic selection(GS) were predicted via a computer simulation study. Results showed that the heritability estimates in this Landrace herd were 0.55(0.02), 0.42(0.01), and 0.12(0.01), for BF, D100, and TNB, respectively. Favorable genetic trends were obtained for D100, BF, and TNB due to direct selection, for ADG and NBA due to indirect selection. Long-term selection against D100 did not improve D30, though they are highly genetically correlated(0.64). Appling GS in such a swine herd, the genetic gain can be increased by 25%, or even larger for traits with low heritability or individuals without phenotypes before selection. It can be concluded that conventional breeding strategy was effective in the herd studied, while applying GS is promising and hence the road ahead in swine breeding. | ZHANG Zhe ZHANG Hao PAN Rong-yang WU Long LI Ya-lan CHEN Zan-mou CAI Geng-yuan LI Jia-qi WU Zhen-fang | 2016 | Journal of Integrative Agriculture2016，15，5： | 3 |
| 16 | Relationship between epidermal growth factor receptor gene mutation and copy number in Chinese patients with non-small cell lung cancer显示文摘Epidermal growth factor receptor(EGFR) gene mutation and copy number are useful predictive markers that guide the selection of non-small cell lung cancer(NSCLC) patients for EGFR-targeting therapy.This study aimed to investigate the correlation between EGFR gene mutation and copy number and clinicopathologic characteristics of Chinese patients with NSCLC.NSCLC specimens collected from 205 patients between November 2009 and January 2011 were selected to detect EGFR gene mutations with real-time polymerase chain reaction(RT-PCR) and to detect EGFR gene copy number with fluorescence in situ hybridization(FISH).EGFR mutations primarily occurred in females,non-smokers,and patients with adenocarinomas(all P < 0.001).Tissues from 128(62%) patients were FISH-positive for EGFR,including 37(18%) with gene amplification and 91(44%) with high polysomy.EGFR gene mutation was correlated with FISH-positive status(R = 0.340,P < 0.001).Multivariate analysis showed that not smoking(OR = 5.910,95% CI = 2.363-14.779,P < 0.001) and having adenocarcinoma(OR = 0.122,95% CI = 0.026-0.581,P = 0.008) were favorable factors for EGFR gene mutation.These results show a high frequency of EGFR FISH positivity in NSCLC tissues from Chinese patients and a significant relevance between EGFR gene mutations and FISH-positive status.Among the FISH-positive samples,EGFR gene mutation occurred more frequently in samples with gene amplification compared to those with high polysomy,suggesting that EGFR mutation and gene amplification should be used as clinical decision parameters to predict response to EGFR-targeting therapy. | Lan-Jun Zhang Ling Cai Zhe Li Wu-Ping Wang Kang Guo Jian-Yong Shao Jun-Ye Wang Hui Yu Tie-Hua Rong | 2012 | Chinese Journal of Cancer2012，31，10： | 3 |
| 17 | Efficacy and Safety of Medium-to-long-term Use of Tolterodine Extended Release with or without Tamsulosin in Patients with Benign Prostate Hyperplasia and Larger Prostate Size: A Double-blind, Placebo-controlled, Randomized Clinical Trial显示文摘 | Jian-Liang Cai Zhe Zhou Yan Yang Yi-Fu Yan Shuo Jing Yan-Qun Na | 2016 | Chinese Medical Journal2016，，24： | 3 |
| 18 | Machine learning algorithms improve the power of phytolith analysis: A case study of the tribe Oryzeae (Poaceae)显示文摘作为微化石的重要来源之一, Phytoliths 广泛地在古植物学相关的研究被使用了,特别在丰富的 phytoliths 被发现的草家庭(Poaceae ) 。尽管有大努力,当 phytoliths 在各种各样的研究被使用时,几挑战留下,包括对 phytolith 形态学的精确描述和在 taxon 鉴定或辨别的 phytolith 特点的有效利用。在这研究,我们在亚科 Bambusoideae 和 Pooideae 从在部落 Oryzeae (亚科 Ehrhartoideae ) 代表七个主要的类的 18 taxa 和五 taxa 分析了超过 1000 件 phytolith 样品。由集中于 Oryzeae,它广泛地以分类和发展史被调查了,我们能在草在更低的分类层次评估 phytoliths 的辨别力量。在 morphometric 分析的帮助下并且由介绍几个机器学习算法,我们发现 87.7% phytolith 样品能在类水平正确地被分类。尽管有稍微不同的表演,所有四个机器学习算法显著地与传统的 phytolith 分析相比在 taxon 鉴定和辨别增加了 phytolith 证据的解决的力量。因此,我们基于机器学习算法建议 phytolith 分析的一条管道,包括数据收集, morphometric 分析,模型大楼,和 taxon 辨别。这里介绍的方法论和管道应该越过植物的不同的组被用于各种各样的研究。这研究提供新卓见进 phytoliths 的利用在进化并且一般来说包含草和植物的生态学研究。 | Zhe Cai Song Ge | 2017 | Journal of Systematics and Evolution2017，55，4： | 3 |
| 19 | Development and validation of a questionnaire-based risk scoring system to identify individuals at high risk for gastric cancer in Chinese populations显示文摘Objective:This study aimed to develop and validate a risk scoring system to identify high-risk individuals carrying malignant lesions in stomach for tailored gastric cancer screening.Methods:A gastric cancer risk scoring system(GC-RSS)was developed based on questionnaire-based predictors for gastric cancer derived from systematic literature review.To assess the capability of this system for discrimination,risk scores for 8,214 and 7,235 outpatient subjects accepting endoscopic examination in two endoscopy centers,and 32,630 participants in a community-based cohort in China were calculated to plot receiver operating characteristic curves and generate area under the curve(AUC).To evaluate the performance of GC-RSS,the screening proportion,sensitivity and detection rate ratio compared to universal screening were used under different risk score cutoff values.Results:GC-RSS comprised nine predictors including advanced age,male gender,low body mass index(<18.5 kg/m^(2)),family history of gastric cancer,cigarette smoking,consumption of alcohol,preference for salty food,irregularity of meals and consumption of preserved food.This tool performed well in determining the risk of malignant gastric lesions with AUCs of 0.763,0.706 and 0.696 in three validation sets.When subjects with risk scores≥5 were evaluated with endoscopy,nearly 50%of these endoscopies could be saved with a detection rate of over 1.5 times achieved.When the cutoff was set at 8,only about 10%of subjects with the highest risk would be offered endoscopy,and detection rates for gastric cancer could be increased 2-4 fold compared to universal screening.Conclusions:An effective questionnaire-based GC-RSS was developed and validated.This tool may play an important role in establishing a tailored screening strategy for gastric cancer in China. | Ren Zhou Hongchen Zheng Mengfei Liu Zhen Liu Chuanhai Guo Hongrui Tian Fangfang Liu Ying Liu Yaqi Pan Huanyu Chen Zhe Hu Hong Cai Zhonghu He Yang Ke | 2021 | Chinese Journal of Cancer Research2021，33，6： | 3 |
| 20 | The mechanism of Naringin-enhanced remyelination after spinal cord injury显示文摘Our previous study revealed that intragastric administration of naringin improved remyelination in rats with spinal cord injury and promoted the recovery of neurological function of the injured spinal cord.This study sought to reveal the mechanisms by which naringin improves oligodendrocyte precursor cell differentiation and maturation,and promotes remyelination.Spinal cord injury was induced in rats by the weight-drop method.Naringin was intragastrically administered daily(20,40 mg/kg) for 4 weeks after spinal cord injury induction.Behavioral assessment,histopathological staining,immunofluorescence spectroscopy,ultrastructural analysis and biochemical assays were employed.Naringin treatment remarkably mitigated demyelination in the white matter,increased the quality of myelinated nerve fibers and myelin sheath thickness,promoted oligodendrocyte precursor cell differentiation by upregulating the expression of NKx2.2 and 2′3′-cyclic nucleotide 3′-phosphodiesterase,and inhibited β-catenin expression and glycogen synthase kinase-3β(GSK-3β) phosphorylation.These findings indicate that naringin treatment regulates oligodendrocyte precursor cell differentiation and promotes remyelination after spinal cord injury through the β-catenin/GSK-3β signaling pathway. | Wei Rong Yong-wei Pan Xu Cai Fei Song Zhe Zhao Song-hua Xiao Cheng Zhang | 2017 | Neural Regeneration Research2017，12，3： | 3 |
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