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| 1 | GNSS-R data acquisition system design and experiment显示文摘In this paper,the design of a GNSS-R data acquisition system is described,including the antenna,RF(radio frequency)front-end and FPGA(field programmable gate array)based logic circuit for data storage interface.Two RF front-ends are connected with the RHCP(right-hand circular polarization)and LHCP(left-hand circular polarization)antenna to collect the direct and reflected signal separately.Both the specifications and the circuit connections are given in detail.Experiments are performed for statics at the seashore and dynamic scenario in the sea using airplanes.The results of the data analysis show that the proposed data acquisition system is suitable for GNSS-R primitive data collection. | YANG DongKai WANG Yan LU Yong LI WeiQiang LI ZiWei | 2010 | Chinese Science Bulletin2010，55，33： | 9 |
| 2 | Exosome-based small RNA delivery:Progress and prospects显示文摘RNA interfering(RNAi), mediated by small interfering RNAs and microRNAs, is currently one of the most promising tools of gene therapy. Small RNAs are capable of inducing specific post-transcriptional gene silencing, providing a potentially effective platform for the treatment of a wide array of diseases. However, similar to other nucleic acid-based drugs,the major hurdle of RNAi therapy is lack of efficient and non-immunogenic delivery vehicles. Currently, viruses, synthetic polymers, and lipid-based carriers are among the most widely studied vehicles for small RNA delivery. However, many drawbacks are reported to be associated with these delivery vehicles. There is a pressing need to replace them with more efficient and better-tolerated approaches. Exosomes secreted from the endocytic compartment of live cells, are a subtype of endogenous extracellular vesicles that transfer genetic and biochemical information among different cells, thus playing an important role in cellcell communication. Recently, accumulating attention has been focused on harnessing exosomes as nanaocarriers for small RNAs delivery. Due to their natural role in shuttling endogenous nucleic acid in our body, exosomes may exhibit higher delivery efficiency, lower immunogenicity, and better compatibility than existing foreign RNA carriers. Importantly,exosomes own intrinsic homing capacity that can guide small RNAs across natural membranous barriers. Moreover, such a capacity can be further improved by adding appropriate targeting moieties. In this manuscript, we briefly review the progress and challenges of RNAi therapy, and discuss the potential of exosomes' applications in small RNA delivery with focus on the most recent advances in exosome-based small RNA delivery for disease therapy. | Mei Lu Haonan Xing Zhe Xun Tianzhi Yang Pingtian Ding Cuifang Cai Dongkai Wang Xiaoyun Zhao | 2018 | Asian Journal of Pharmaceutical Sciences2018，13，1： | 8 |
| 3 | Familial Parkinson's Disease-Associated L166P Mutant DJ-1 is Cleaved by Mitochondrial Serine Protease Omi/HtrA2显示文摘Parkinson's disease(PD) is the most common neurodegenerative movement disorder. Mutations in the DJ-1, including L166 P, are responsible for recessive earlyonset PD. Many lines of evidence have shown that L166 P is not only a loss-of-function mutant, but also a proapoptotic-like protein that results in mitochondrial dysfunction. L166 P has been reported to be unstable and to mislocalize to mitochondria. However, the mechanisms underlying the instability of L166 P compared to wild-type DJ-1 remain largely unknown. Here, we showed that Omi/Htr A2, a mitochondrial serine protease that has also been linked to the pathogenesis of PD, contributed to L166 P instability. Omi directly interacted with and cleaved L166 P in mitochondria to decrease the L166 P level. However,Omi did not bind and cleave wild-type DJ-1. Moreover,Omi cleaved L166 P at both serine residues 3 and 121,while L166 P-induced cell death under H_2O_2 treatment was alleviated by over-expression of Omi. Our data reveal a bridge between DJ-1 and Omi, two PD-associated geneticfactors, which contributes to our understanding of the pathogenesis of PD. | Kai Fu Yanfei Wang Dongkai Guo Guanghui Wang Haigang Ren | 2017 | Neuroscience Bulletin2017，33，6： | 6 |
| 4 | Cholesterol-tuned liposomal membrane rigidity directs tumor penetration and anti-tumor effect显示文摘Recently, liposomes have been widely used in cancer therapeutics, but their anti-tumor effects are suboptimal due to limited tumor penetration. To solve this problem, researchers have made significant efforts to optimize liposomal diameters and potentials, but little attention has been paid to liposomal membrane rigidity. Herein, we sought to demonstrate the effects of cholesterol-tuned liposomal membrane rigidity on tumor penetration and anti-tumor effects. In this study, liposomes composed of hydrogenated soybean phospholipids(HSPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000](DSPE-PEG2000) and different concentrations of cholesterol were prepared. It was revealed that liposomal membrane rigidity decreased with the addition of cholesterol. Moderate cholesterol content conferred excellent diffusivity to liposomes in simulated diffusion medium, while excessive cholesterol limited the diffusion process. We concluded that the differences of the diffusion rates likely stemmed from the alterations in liposomal membrane rigidity, with moderate rigidity leading to improved diffusion. Next, the in vitro tumor penetration and the in vivo anti-tumor effects were analyzed. The results showed that liposomes with moderate rigidity gained excellent tumor penetration and enhanced anti-tumor effects. These findings illustrate a feasible and effective way to improve tumor penetration and therapeutic efficacy of liposomes by changing the cholesterol content, and highlight the importance of liposomal membrane rigidity. | Hangyi Wu Miaorong Yu Yunqiu Miao Shufang He Zhuo Dai Wenyi Song Yuan Liu Sha Song Ejaj Ahmad Dongkai Wang Yong Gan | 2019 | Acta Pharmaceutica Sinica B2019，9，4： | 5 |
| 5 | Standard pancreatoduodenectomy versus extended pancreatoduodenectomy with modified retroperitoneal nerve resection in patients with pancreatic head cancer:a multicenter randomized controlled trial显示文摘Background:The extent of pancreatoduodenectomy for pancreatic head cancer remains controversial,and more high-level clinical evidence is needed.This study aimed to evaluate the outcome of extended pancreatoduodenectomy(EPD)with retroperitoneal nerve resection in pancreatic head cancer.Methods:This multicenter randomized trial was performed at 6 Chinese highvolume hospitals that enrolled patients between October 3,2012,and September 21,2017.Four hundred patients with stage I or II pancreatic head cancer and without specific pancreatic cancer treatments(preoperative chemotherapy or chemoradiation)within three months were randomly assigned to undergo standard pancreatoduodenectomy(SPD)or EPD,with the latter followed by dissection of additional lymph nodes(LNs),nerves and soft tissues 270◦on the right side surrounding the superior mesenteric artery and celiac axis.The primary endpoint was overall survival(OS)by intention-to-treat(ITT).The secondary endpoints were disease-free survival(DFS),mortality,morbidity,and postoperative pain intensity.Results:TheR1 ratewas slightly lower with EPD(8.46%)thanwith SPD(12.56%).The morbidity and mortality rates were similar between the two groups.The median OS was similar in the EPD and SPD groups by ITT in the whole study cohort(23.0 vs.20.2 months,P=0.100),while the median DFS was superior in the EPD group(16.1 vs.13.2 months,P=0.031).Patients with preoperative CA19–9<200.0 U/mL had significantly improved OS and DFS with EPD(EPD vs.SPD,30.8 vs.20.9 months,P=0.009;23.4 vs.13.5 months,P<0.001).The EPD group exhibited significantly lower locoregional(16.48%vs.35.20%,P<0.001)andmesenteric LNrecurrence rates(3.98%vs.10.06%,P=0.022).The EPD group exhibited less back pain 6 months postoperation than the SPD group.Conclusions:EPD for pancreatic head cancer did not significantly improve OS,but patients with EPD treatment had significantly improved DFS.In the subgroup analysis,improvements in bothOS and DFS in the EPD armwere observed in patients with preoperative CA19–9<200.0 U/mL.EPD could be used as an effective surgical procedure for patients with pancreatic head cancer,especially those with preoperative CA19–9<200.0 U/mL. | Qing Lin Shangyou Zheng Xianjun Yu Meifu Chen Yu Zhou Quanbo Zhou Chonghui Hu Jing Gu Zhongdong Xu LinWang Yimin Liu Qingyu Liu MinWang Guolin Li He Cheng Dongkai Zhou Guodong Liu Zhiqiang Fu Yu Long Yixiong Li Weilin Wang Renyi Qin Zhihua Li Rufu Chen | 2023 | Cancer Communications2023，43，2： | 3 |
| 6 | Notch Effect and Frequency Compensation of Dual-Sensor OBC Data in Shallow Water显示文摘Reverberation is significant in shallow water and produces obvious notches in OBC spectra.It also degrades the quality of sections and increases the difficulty of processing and interpretation.This article presents the relationship between notch,shooting depth,and seabed depth based on the seismic convolution model.Forward modelling based on wave equation theory is used to verify this relationship.Dual-sensor summation is applied to suppress receiver-side multiples and remove notches according to the opposite response of geophones and hydrophones to down-going wave fields based on a detailed analysis of the OBC technique.The good results obtained in practical applications reveal the effectiveness of this method. | Dongkai Wang Siyou Tong Huaishan Liu Weiqiang Zhu | 2015 | Journal of Earth Science2015，26，4： | 2 |
| 7 | Meiotic Chromosome Behavior in a Human Male t(8;15)Carrier显示文摘Reciprocal translocation is one of the most common structural chromosomal rearrangements in human beings;it is widely recognized to be associated with male infertility.This association is mainly based on the abnormal chromosome behavior of the translocated chromosomes and sex chromosomes during meiosis prophase I in reciprocal translocation carriers.However,the underlying mechanisms are not completely known.Here we report a reciprocal translocation carrier of t(8;15),who is oligozoospermic due to apoptosis of primary spermatocytes and to premature germ cell desquamation from seminiferous tubules.Further analysis showed abnormal synapsis and recombination frequency in this patient,indicating a connection between chromosome behavior and apoptosis of primary spermatocytes.We also compared these observations with recently reported findings on spermatogenesis defects in reciprocal translocation carriers,and discuss the possible mechanisms underlying bom common and unique phenotypes of reciprocal translocations involving different chromosomes with the aim of further understanding the regulation of human spermatogenesis. | Hanwei Jiang Liu Wang Yingxia Cui Zhipeng Xu Tonghang Guo Dongkai Cheng Peng Xu Wen Yu Qinghua Shi | 2014 | Journal of Genetics and Genomics2014，41，3： | 2 |
| 8 | Dual-responsive nanovaccine for cytosolic delivery of antigens to boost cellular immune responses and cancer immunotherapy显示文摘Cancer vaccine contributing to the success of the treatment and prevention of tumors has attracted a huge attention as a strategy for tumor immunotherapy in recent years.A major challenge of cancer vaccine is to target cytosols of dendritic cells(DCs)in the lymph nodes(LNs)to enhance efficiency of antigen cross-presentation,which elicits high levels of cytotoxic T-lymphocytes to destruct tumor cells.Here,we address this issue by conjugating ovalbumin(OVA)to PEG-PCL using disulfide bond(-ss-),and the degradable pH-responsive polymer-PEI-PCL as delivery carrier.In addition,the mol ratio of PEG-PCL to PEI-PCL in the mixed micelles was tailored to deliver the OVA to LNs.Subsequently,CpG ODN_(1826),a TLR-9 agonist,was further introduced into a mixed micelle of 30nm or less as a unique tumor vaccine.Importantly,the results demonstrated the mixed micelles with 1:1mol of PCL-PEG and PCL-PEI can effectively migrate to distal LNs where antigen were efficiently captured by DCs,meanwhile,OVA was modified to the surface of mixed micelles via disulfide bonds(-ss-)for promotion efficiency of antigen cross-presentation.More surprisingly,combination of tumor vaccine with anti-PD-1,the therapy of ectopic melanoma(B16-OVA)and lung metastasis melanoma(B16-OVA)is excellent therapeutic effect.Taken together,our works offers a novel strategy for the cytosol delivery of antigens to achieve potent cancer immunotherapy. | Yang Sui Ji Li Jiqiang Qu Ting Fang Hongyan Zhang Jian Zhang Zheran Wang Mingyu Xia Yinghui Dai Dongkai Wang | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，4： | 1 |
| 9 | Design, Synthesis and Fungicidal Activity of Novel Strobilurin-l,2,4-Triazole Derivatives Containing Furan or Thiophene Rings显示文摘A series of strobilurin-l,2,4-triazole derivatives containing a furan or thiophen ring was designed andsynthesized. The structures of the compounds were characterized by 1H NMR, 13C NMR and HRMS spectra. Thebioassays indicated that the fungicidal activities of compounds 10b(EC50=14.82 mg/L) and 10c(EC50=18.72 mg/L)against Cercospora arachidicola Hori in vitro were much higher than that of Azoxystrobin as control(EC50=40.54mg/L) and the fungicidal activities of compounds 10u(EC50=8.66 rag/L) and 10n(EC50=9.89 rag/L) against Rhizotoniacerealis in vitro were higher than that of the same control(EC50=10.86 rag/L). Compounds 10b, 10c, 1011 and 10kcould be considered as the leading compounds for further investigation. | LIU Yang LIU Ming ZHANG Dongkai HUA Xuewen WANG Baolei ZHOU Sha LI Zhengming | 2016 | Chemical Research in Chinese Universities2016，32，6： | 1 |
| 10 | Forecasting Chinese Corporate Bond Defaults: Comparative Study of Market- vs. Accounting-Based Models显示文摘This paper provides the first empirical study on bond defaults in the Chinese market.It overcomes the deficiencies of existing methods,which suffer from lack of actual default data for back testing.With newly available bond default data,we analyze the roles of market variables against accounting variables under various models.While we find that Merton's market-based structural model and KMV's Distance to Default exhibit languid discriminating power compared with hazard models that have carefully constructed predictors,other market variables carry significant information about bond defaults and could help improve on models with only the accounting variables.This implies that the collective intelligence of the market could somehow mitigate the distortion caused by misreported accounting information.Further,model performance can be significantly improved by adding predicting variables that link an individual financial measure to the broader market performance,such as the relative margin—a business environment proxy introduced in this study.We not only shed light on the default behavior of the Chinese bond market,but also provide a promising approach to improve the variable selection process. | Michael Peng Dongkai Jiang Yingjie Wang | 2019 | Frontiers of Economics in China-Selected Publications from Chinese Universities2019，14，4： | 1 |
| 11 | Hepatitis B virus X protein inhibits p53-mediated upregulation of mitofusin-2 in hepatocellular carcinoma cells显示文摘 | Weilin Wang Dongkai Zhou Jianfeng Wei Zehui Wu Xiaofei Cheng Qiang Sun Haiyang Xie Lin Zhou Shusen Zheng | 2012 | Biochemical and Biophysical Research Communications2012，，2： | 1 |
| 12 | New advances in monitoring and triggering of mechanical ventilation显示文摘Mechanical ventilation has been widely used in intensive care unit(ICU)to provide adequate air exchange to patients with respiratory difficulty.It helps patients with a ventilator that presses air into patients’lungs at triggering time.As a key therapy in ICU,mechanical ventilation is of great significance in clinical research of respiratory treatment.For example,simulation research of mechanical ventilation can be made through dynamics equations of pneumatic | SHI Yan ZHANG BoLun WANG ZeDan CAI MaoLin SHEN DongKai | 2016 | Science China(Technological Sciences)2016，59，11： | 1 |
| 13 | Robust backstepping control based DRNN for flight simulator 显示文摘 | Shen Dongkai Wang Jingjing Liu Zhenghua | 2010 | Advanced Materials Research2010，，： | 1 |
| 14 | Regulation of autophagic flux by CHIP显示文摘Autophagy is a major degradation system which processes substrates through the steps of autophagosome formation, autophagosome-lysosome fusion, and substrate degradation. Aberrant autophagic flux is present in many pathological conditions including neurodegeneration and tumors. CHIP/STUB1, an E3 ligase, plays an important role in neurodegeneration. In this study, we identified the regulation of autophagic flux by CHIP(carboxy-terminus of Hsc70-interacting protein). Knockdown of CHIP induced autophagosome formation through increasing the PTEN protein level and decreasing the AKT/m TOR activity as well as decreasing phosphorylation of ULK1 on Ser757. However, degradation of the autophagic substrate p62 was disturbed by knockdown of CHIP, suggesting an abnormality of autophagic flux. Furthermore, knockdown of CHIP increased the susceptibility of cells to autophagic cell death induced by bafilomycin A1. Thus, our data suggest that CHIP plays roles in the regulation of autophagic flux. | Dongkai Guo Zheng Ying Hongfeng Wang Dong Chen Feng Gao Haigang Ren Guanghui Wang | 2015 | Neuroscience Bulletin2015，31，4： | 1 |
| 15 | Emerging theory and phenomena in thermal conduction: A selective review显示文摘Recently, emerging phonon phenomena have been discovered and rapidly developed, which have become an active hot research topic. In this review article, we present state-of-the-art advances in several fascinating phonon transport phenomena. First, we summarize the recent progress on the wave nature of phonons, including phonon coherence and its effects on thermal conductivity and the topological properties of phonons. Then, we discuss the particle nature of phonons, including the weak coupling of phonons and the high-order phonon anharmonicity. Finally, we present the summary and a brief outlook. This review presents the advanced understanding of some emerging phonon phenomena in solid materials, which provides new opportunities for further advancement in a wide variety of applications. | Jie Chen Jia He Dongkai Pan Xiaotian Wang Nuo Yang Jiaojiao Zhu Shengyuan A.Yang Gang Zhang | 2022 | Science China(Physics,Mechanics & Astronomy)2022，65，11： | 1 |
| 16 | Discovery decision rules in incomplete decision tables显示文摘 | Wang Dongkai Liang Liang | 2001 | Computer Science2001，28，5： | 1 |
| 17 | Applications and recent advances in transdermal drug delivery systems for the treatment of rheumatoid arthritis显示文摘Rheumatoid arthritis is a chronic,systemic autoimmune disease predominantly based on joint lesions with an extremely high disability and deformity rate.Several drugs have been used for the treatment of rheumatoid arthritis,but their use is limited by suboptimal bioavailability,serious adverse effects,and nonnegligible first-pass effects.In contrast,transdermal drug delivery systems(TDDSs)can avoid these drawbacks and improve patient compliance,making them a promising option for the treatment of rheumatoid arthritis(RA).Of course,TDDSs also face unique challenges,as the physiological barrier of the skin makes drug delivery somewhat limited.To overcome this barrier and maximize drug delivery efficiency,TDDSs have evolved in terms of the principle of transdermal facilitation and transdermal facilitation technology,and different generations of TDDSs have been derived,which have significantly improved transdermal efficiency and even achieved individualized controlled drug delivery.In this review,we summarize the different generations of transdermal drug delivery systems,the corresponding transdermal strategies,and their applications in the treatment of RA. | Yuyi Xu Ming Zhao Jinxue Cao Ting Fang Jian Zhang Yanli Zhen Fangling Wu Xiaohui Yu Yaming Liu Ji Li Dongkai Wang | 2023 | Acta Pharmaceutica Sinica B2023，13，11： | 0 |
| 18 | Liquisolid technique and its applications in pharmaceutics显示文摘Most of the newly developed drug candidates are lipophilic and poorly water-soluble. Enhancing the dissolution and bioavailability of these drugs is a major challenge for the pharmaceutical industry. Liquisolid technique, which is based on the conversion of the drug in liquid state into an apparently dry, non-adherent, free flowing and compressible powder,is a novel and advanced approach to tackle the issue. The objective of this article is to present an overview of liquisolid technique and summarize the progress of its applications in pharmaceutics. Low cost, simple processing and great potentials in industrial production are main advantages of this approach. In addition to the enhancement of dissolution rate of poorly water-soluble drugs, this technique is also a fairly new technique to effectively retard drug release. Furthermore, liquisolid technique has been investigated as a tool to minimize the effect of pH variation on drug release and as a promising alternative to conventional coating for the improvement of drug photostability in solid dosage forms. Overall, liquisolid technique is a newly developed and promising tool for enhancing drug dissolution and sustaining drug release, and its potential applications in pharmaceutics are still being broadened. | Mei Lu Haonan Xing Jingzheng Jiang Xiao Chen Tianzhi Yang Dongkai Wang Pingtian Ding | 2017 | Asian Journal of Pharmaceutical Sciences2017，12，2： | 0 |
| 19 | Inflammatory risk stratification individualizes anti-inflammatory pharmacotherapy for acute type A aortic dissection显示文摘The systemic benefits of anti-inflammatory pharmacotherapy vary across cardiovascular diseases in clinical practice.We aimed to evaluate the application of artificial intelligence to acute type A aortic dissection(ATAAD)patients to determine the optimal target population who would benefit from urinary trypsin inhibitor use(ulinastatin).Patient characteristics at admission in the Chinese multicenter 5A study database(2016-2022)were used to develop an inflammatory risk model to predict multiple organ dysfunction syndrome(MODS). | Hong Liu Haiyang Li Lu Han Yingyuan Zhang Ying Wu Liang Hong Jinong Yang Jisheng Zhong Yuqi Wang Dongkai Wu Guoliang Fan Junquan Chen Shengqiang Zhang Xingxing Peng Zhihua Zeng Zhiwei Tang Zhanjie Lu Lizhong Sun Sichong Qian Yongfeng Shao Hongjia Zhang on behalf of the Additive Anti-inflammatory Action for Aortopathy&Arteriopathy(A)Investigators | 2023 | The Innovation2023，4，4： | 0 |
| 20 | Dimensionless Study on Secretion Clearance of a Pressure Controlled Mechanical Ventilation System with Double Lungs显示文摘A pressure controlled mechanical ventilator with an automatic secretion clearance function can improve secretion clearance safely and efficiently.Studies on secretion clearance by pressure controlled systems show that these are suited for clinical applications.However,these studies are based on a single lung electric model and neglect the coupling between the two lungs.The research methods applied are too complex for the analysis of a multi-parameter system.In order to understand the functioning of the human respiratory system,this paper develops a dimensionless mathematical model of doublelung mechanical ventilation system with a secretion clearance function.An experiment is designed to verify the mathematical model through comparison of dimensionless experimental data and dimensionless simulation data.Finally,the coupling between the two lungs is studied,and an orthogonal experiment designed to identify the impact of each parameter on the system. | Dongkai Shen Qian Zhang Yixuan Wang Huiqing Ge Zujin Luo | 2018 | Computer Modeling in Engineering & Sciences2018，，2： | 0 |
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