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| 1 | Modeling study of regional severe hazes over mid-eastern China in January 2013 and its implications on pollution prevention and control显示文摘The Nested Air Quality Prediction Model System(NAQPMS)was used to investigate the temporal and spatial variations of PM2.5over tropospheric central eastern China in January 2013.The impact of regional transport and its implications on pollution prevention and control were also examined.Comparison between simulated and observed PM2.5showed NAQPMS was able to reproduce the evolution of PM2.5during heavy haze episodes.The results indicated that regional transport of PM2.5played an important role in regional haze episodes in the city cluster including Hebei,Beijing and Tianjin(HBT).The cross-city clusters transport outside HBT and transport among cities inside HBT contributed 20%–35%and 26%–35%of PM2.5as compared with local emission,in HBT respectively.To meet the Air Quality Standards for Grade II,90%,90%and65%of emissions would have to be cut down in Hebei,Tianjin and Beijing,if non-control strategy was taken in the surrounding city clusters of HBT.This implicated that control of emissions in one city cluster is not sufficient to reduce regional haze events,and joint efforts among city clusters are essential.Besides regional transports,two-way feedback between boundary-layer evolution and PM2.5also significantly contributed to the formation of heavy hazes,which contributed 30%of monthly average PM2.5concentration in HBT. | WANG ZiFa LI Jie WANG Zhe YANG WenYi TANG Xiao GE BaoZhu YAN PinZhong ZHU LiLi CHEN XueShun CHEN HuanSheng WAND Wei LI JianJun LIU Bing WANG XiaoYan WAND Wei ZHAO YiLin LU Ning SU DeBin | 2014 | Science China Earth Sciences2014，57，1： | 106 |
| 2 | Traditional herbal medicine in preventing recurrence after resection of small hepatocellular carcinoma: a multicenter randomized controlled trial显示文摘BACKGROUND: Disease recurrence is a main challenge in treatment of hepatocellular carcinoma (HCC). There is no generally accepted method for preventing recurrence of HCC after resection. OBJECTIVE: To compare the efficacy of a traditional herbal medicine (THM) regimen and transarterial chemoembolization (TACE) in preventing recurrence in post-resection patients with small HCC. DESIGN, SETTING, PARTICIPANTS AND INTERVENTIONS: This is a multicenter, open-label, randomized, controlled study, which was undertaken in five centers of China. A total of 379 patients who met the eligibility criteria and underwent randomization were enrolled in this trial. One hundred and eighty-eight patients were assigned to the THM group and received Cinobufacini injection and Jiedu Granule, and the other 191 patients were assigned to the TACE group and received one single course of TACE. MAIN OUTCOME MEASURES: Primary outcome measures were the annual recurrence rate and the time to recurrence. Incidence of adverse events was regarded as the secondary outcome measure. RESULTS: Among the 364 patients who were included in the intention-to-treat analysis, 67 patients of the THM group and 87 of the TACE group had recurrence, with a hazard ratio of 0.695 (P = 0.048). Median recurrence-free survival of the patients in the THM and TACE groups was 46.89 and 34.49 months, respectively. Recurrence rates at 1, 2 and 3 years were 17.7%, 33.0% and 43.5% for the THM group, and 28.8%, 42.5% and 54.0% for the TACE group, respectively (P = 0.026). Multivariate analysis indicated that the THM regimen had a big advantage for prolonging the recurrence-free survival. Adverse events were mild and abnormality of laboratory indices of the two groups were similar. CONCLUSION: In comparison with TACE therapy, the THM regimen was associated with diminished risk of recurrence of small-sized HCC after resection, with comparable adverse events. TRIAL REGISTRTION IDENTIFIER: This trial was registered in the Chinese Clinical Trial Registry with the identifier ChiCTR-TRC-07000033. | Xiao-feng Zhai Zhe Chen Bai Li Feng Shen Jia Fan Wei-ping Zhou Yun-ke Yang Jing Xu Xiao Qin Le-qun Li Chang-quan Ling | 2013 | Journal of Integrative Medicine2013，11，2： | 49 |
| 3 | Pathogenicity of a currently circulating Chinese variant pseudorabies virus in pigs显示文摘AIM:To test the pathogenicity of pseudorabies virus(PRV)variant HN1201 and compare its pathogenicity with a classical PRV Fa strain.METHODS:The pathogenicity of the newly-emerging PRV variant HN1201 was evaluated by different inoculating routes,virus loads,and ages of pigs.The classical PRV Fa strain was then used to compare with HN1201 to determine pathogenicity.Clinical symptoms after virus infection were recorded daily and average daily body weight was used to measure the growth performance of pigs.At necropsy,gross pathology and histopathology were used to evaluate the severity of tissue damage caused by virus infection.RESULTS:The results showed that the efficient infection method of RPV HN1201 was via intranasal inoculation at 107 TCID50,and that the virus has high pathogenicity to 35-to 127-d old pigs.Compared with Fa strain,pigs infected with HN1201 showed more severe clinical symptoms and pathological lesions.Immunochemistry results revealed HN1201 had more abundant antigen distribution in extensive organs.CONCLUSION:All of the above results suggest that PRV variant HN1201 was more pathogenic to pigs than the classical Fa strain. | Qing-Yuan Yang Zhe Sun Fei-Fei Tan Ling-Hua Guo Yu-Zhou Wang Juan Wang Zhi-Yan Wang Li-Lin Wang Xiang-Dong Li Yan Xiao Ke-Gong Tian | 2016 | World Journal of Virology2016，5，1： | 31 |
| 4 | Safflower yellow injection combined with conventional therapy in treating unstable angina pectoris:a meta-analysis显示文摘OBJECTIVE:To evaluate the clinical efficacy of safflower yellow injection combined with conventional therapy in treating unstable angina pectoris.METHODS:We searched online databases:Chinese journal full-text database,China National Knowledge Infrastructure,Wanfang database,Chinese journal full-text database,Pubmed,ScienceDirect,Embase,and the Cochrane Library with manual-screening of relevant literature.Eligible randomized controlled trials(RCT)on angina pectoris were included.We conducted meta-analysis using the RevMan 5.1 software from The Cochrane Collaboration.We treated the relief rate of angina symptoms and electrocardiograph(ECG)as evaluation.RESULTS:Seven articles,including in 1134 patients,were enrolled after the evaluation.Therewas no significant heterogeneity among the studies(χ2=1.08,df=6,P=0.98,I2=0%).The safflower yellow injection with conventional therapy has a higher effective rate than the control group in relieving the symptoms of angina pectoris[odds ratio(OR)=2.95,95%(CI)(1.81,4.81)]and improving ischemic ECG[OR=2.85,95%CI(1.67,4.86)].The difference was statistically significant in the'80 mg dosage'and'100 mg dosage'subgroups(P<0.05)for improving clinical symptoms and ECG.The funnel graphic was nearly symmetrical.Sensitivity analysis suggested that the results were stable.CONCLUSION:Safflower yellow injection as an adjunct therapy with conventional drugs shows advantages in easing the clinical symptoms of unstable angina and improving ECG over basic therapy alone.However,the conclusions should be interpreted with care until more high-quality RCTs are reported.2013 JTCM.All rights reserved. | Dezhao Kong Wei Xia Zhe Zhang Lei Xiao Dongchao Yuan Yue Liu Guanlin Yang | 2013 | Journal of Traditional Chinese Medicine2013，33，5： | 20 |
| 5 | Exploring an Integrative Therapy for Treating COVID-19:A Randomized Controlled Trial显示文摘Objectives:To develop a new Chinese medicine(CM)-based drug and to evaluate its safety and effect for suppressing acute respiratory distress syndrome(ARDS)in COVID-19 patients.Methods:A putative ARDS-suppressing drug Keguan-1 was first developed and then evaluated by a randomized,controlled two-arm trial.The two arms of the trial consist of a control therapy(alpha interferon inhalation,50(x g twice daily;and lopinavir/ritonavir,400 and 100 mg twice daily,respectively)and a testing therapy(control therapy plus Keguan-119.4 g twice daily)by random number table at 1:1 ratio with 24 cases each group.After 2-week treatment,adverse events,time to fever resolution,ARDS development,and lung injury on newly diagnosed COVID-19 patients were assessed.Results:An analysis of the data from the first 30 participants showed that the control arm and the testing arm did not exhibit any significant differences in terms of adverse events.Based on this result,the study was expanded to include a total of 48 participants(24 cases each arm).The results show that compared with the control arm,the testing arm exhibited a significant improvement in time to fever resolution(P=0.035),and a significant reduction in the development of ARDS(P=0.048).Conclusions:Keguan-1-based integrative therapy was safe and superior to the standard therapy in suppressing the development of ARDS in COVID-19 patients.(Trial registration No.NCT 04251871 at www.clinicaltrials.gov). | WANG Jia-bo WANG Zhong-xia JING Jing ZHAO Peng DONG Jing-hui ZHOU Yong-feng YANG Guang NIU Ming ZHAO Xu JIANG Tian-jun Bl Jing-feng XU Zhe ZHANG Ping WU Dan BAI Zhao-fang GUO Yu-ming YU Si-miao SUN Yong-qiang ZHANG Zi-teng ZHAN Xiao-yan LI Peng-yan DING Jin-biao ZHAO Peng-fei SONG Xue-ai TANG Jian-yuan HE Dong-chu CHEN Zhu QIN En-qiang WANG Rui-lin XIAO Xiao-he | 2020 | Chinese Journal of Integrative Medicine2020，26，9： | 18 |
| 6 | Individualized management of pregnant women with high hepatitis B virus DNA levels显示文摘Hepatitis B is a major health concern in the Asia-Pacific region,and is endemic in China,Southeast Asia,and Africa.Chronic hepatitis B virus(HBV)infection may cause hepatic cirrhosis and liver cancer.It is estimated that there are more than 350 million chronic HBV carriers worldwide,of whom approximately one quarter will die of chronic hepatitis B-related liver diseases.HBV is transmitted horizontally through blood and blood products or by sexual transmission,and vertically from mother to infant.Perinatal infection is the predominant mode of transmission in countries with a high prevalence of hepatitis B surface antigen(HBsAg)carriage,and perinatal transmission leads to high rates of chronic infection.Therefore,it is important to prevent the mother-to-child transmission(MTCT)of HBV.Research has shown that pregnant women with high HBV DNA levels have an increased risk of MTCT.However,most of the obstetrics guidelines do not make a distinction between pregnant women with high HBV DNA levels and those who are HBsAg positive only.This review addresses the management of pregnant women with high levels of HBV viremia,in terms of antiviral therapy,use of hepatitis B immunoglobulin(HBIG),the combined application of hepatitis B vaccine and HBIG,choice of delivery mode and feeding practices. | Zhao Zhang Chao Chen Zhe Li Ying-Hua Wu Xiao-Min Xiao | 2014 | World Journal of Gastroenterology2014，20，34： | 15 |
| 7 | Short-term Prognosis of Fragmented QRS Complex in Patients with Non-ST Elevated Acute Myocardial Infarction显示文摘 | Min Li Xiao Wang Shu-Hua Mi Zhe Chi Qing Chen Xin Zhao Shao-Ping Nie | 2016 | Chinese Medical Journal2016，，5： | 14 |
| 8 | Clinical practice guidelines on liver transplantation for hepatocellular carcinoma in China(2018 edition)显示文摘Introduction Over 300000 people in China die each year of hepatocellular carcinoma(HCC),which accounts for approximately half of HCCrelated deaths worldwide.Liver transplantation(LT)is generally recognized as one of the most effective therapeutic approaches for end-stage liver diseases.Since the beginning of the second LT boom in the 1990s,LT in China has been developed rapidly with professional and large-scale trends,and it is approaching or has reached the level of developed countries in terms of quantity and quality.According to the China Liver Transplant Registry,the number of transplants for HCC accounted for 36.8%of the total number of LT cases during the past 5 years in the mainland of China.In order to develop an effective,safe and standardized protocol to guide the national LT practice,the clinical guidelines of LT for HCC was launched in 2014 by multidisciplinary experts from Chinese Society of Organ Transplantation,Chinese Medical Association and Chinese Association of Organ Transplantation,Chinese Medical Doctor Association.Recently,there have been new clinical and scientific advances in the field of LT and to keep abreast of these achievements,the original clinical practice guidelines need to be updated. | Xiao Xu Jun Chen Qiang Wei Zhi-Kun Liu a Zhe Yang Ming Zhang Guo-Ying Wang Jie Gao Zhao-Xu Yang Wen-Yuan Guo Tong-Hai Xing Zhou Shao Qin-Fen Xie Shu-Sen Zheng | 2019 | Hepatobiliary & Pancreatic Diseases International2019，18，4： | 14 |
| 9 | Breakdown Properties of Nano-SiO2/LDPE Composite显示文摘聚合物纳米复合介质的击穿强度与纳米填充颗粒的质量分数、粒径和表面处理密切相关。以不同表面处理的纳米SiO2颗粒为填料,制备了不同填充质量分数、粒径的纳米SiO2/低密度聚乙烯(low density polyethylene,LDPE)复合介质,测试了其在交流、直流正极性和直流负极性3种不同类型电场下的击穿场强。结果表明:在所研究范围内,填充纳米SiO2颗粒可以提高低密度聚乙烯的击穿场强,并且随着填充质量分数的增加,复合介质的击穿场强有升高的趋势。纳米颗粒填充质量分数相同时,在12~16am粒径下,复合介质的击穿场强有随纳米SiO2粒径的升高而降低的趋势;在7nm小粒径下,可能由于纳米颗粒容易团聚,导致击穿场强较粒径大时要低。另外,纳米SiO2颗粒表面经疏水性处理后,能够有效提高复合介质的击穿场强。 | LAN Li WU Jiandong JI Zheqiang WANG Qiaohua LI Zhe XIAO Dengming YIN Yi | 2012 | 中国电机工程学报2012，32，13： | 14 |
| 10 | α-Asarone suppresses the proliferation and migration of ASMCs through targeting the IncRNA-PVT1/miR-203a/E2F3 signal pathway in RSV-infected rats显示文摘气喘是长期的煽动性的肺的疾病,呼吸 syncytial 病毒(RSV ) 感染是在婴儿和小孩子的更低的呼吸的道病的一个普通原因。-Asarone 介绍许多药理学效果并且被表明了在对待气喘有用。然而,在感染 RSV 的气喘的 -asarone 的功能的机制没被调查。长非编码的 RNA (lncRNAs ) 被报导了在许多生物过程起关键作用。尽管许多 lncRNAs 被描绘了,很少在气喘被报导,特别在导致 RSV 的气喘。当前,一条新奇 post-transcriptional 规定在哪个 lncRNAs 作为竞争内长的 RNA (ceRNAs ) 工作竞争地擦掉 miRNAs 被建议了,从而调整目标基因。在现在的学习,我们建立了一个感染 RSV 的 Sprague-Dawley 老鼠模型并且证明 lncRNA-PVT1 在对待导致 RSV 的气喘,和 lncRNA-PVT1 涉及 -asarone 的机制由作为竞争地擦掉 miR-203a 的 ceRNA 工作调整 E2F3 的表示。 | Xiao Yu Zhe Zhe Binqing Tang Shaobin Li Ling Tang Yingen Wu Xiaorong Chen Hong Fang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，7： | 14 |
| 11 | Temperature effect on the kinetics of persulfate oxidation of p-chloroaniline显示文摘在水的答案的 p-chloroaniline (PCA ) 的便于热的 persulfate 氧化的动力学以五个不同温度条件并且在四不同氧化剂集中被测量。当 persulfate 是过多的时, PCA 降级被发现跟随一个伪 first-order 腐烂模型。由在 pH 的 persulfate (50 mmol/L ) 的 PCA 降级的伪 first-order 率常数 7.0 是 0. | Yong Qing Zhang Xiao Zhe Du Wei Lin Huang | 2011 | Chinese Chemical Letters2011，22，3： | 13 |
| 12 | Rare earth elements and carbon isotope geochemistry of the Doushantuo Formation in South China:Implication for middle Ediacaran shallow marine redox conditions显示文摘The middle Ediacaran Shuram excursion, the largest negative δ 13 C carb excursion in Earth history, has been interpreted as indirect evidence for episodic oxidation and remineralization of deep ocean DOC (dissolved organic carbon). It has been hypothesized that such oxidation event may have occurred when anoxic DOC-laden deep water was brought to shallow shelves during oceanic upwelling, which is expected to cause localized anoxia in shallow environments. To test this prediction, we systematically analyzed rare earth elements (REE) and δ 13 C carb of the upper Doushantuo Formation carbonates in the Yangtze Gorges area of South China, which were deposited in an inner shelf environment and record a large negative δ 13 C carb excursion correlated to the Shuram event. The REE data show a significant positive shift in Ce/Ce* values, synchronous with a pronounced negative δ 13 C carb shift. This positive Ce/Ce* shift is interpreted to represent an oceanic anoxia event in shallow shelf environments, which may have been caused by the upwelling or impingement of oxygen-depleted and 12 C-enriched deep water onto shelves. This anoxia event coincides with a sharp decline in the abundance and diversity of Ediacaran acanthomorphic acritarchs, raising the possibility that these two geobiological events may be causally related. | ZHOU ChuanMing JIANG ShaoYong XIAO ShuHai CHEN Zhe YUAN XunLai | 2012 | Chinese Science Bulletin2012，57，16： | 11 |
| 13 | Allele-defined genome reveals biallelic differentiation during cassava evolution显示文摘Dear Editor,Generation of heterozygous genomes by hybridization between or within species can help maintain plant diversity and serve as a potential source of new species(Baek et al.,2018).Moreover,genomic heterozygosity is associated with genomic coadaptation,developmental stability,and heterosis.Accurate definition of alleles in haplotypes is necessary to precisely characterize allelic variation controlling agriculturally important traits(Shi et al.,2019).Currently,most released genomes have mosaic assembly of haplotypes due to random selection or collapse of alleles during genome assembly(Shi et al.,2019),which masked allelic variation and functional differentiation of divergent alleles in heterozygous species. | Wei Hu Changmian Ji Haitao shi Zhe Liang Zehong Ding Jianqiu Ye Wenjun Ou Gang Zhou Weiwei Tie Yan Yan Jinghao Yang Xiaoying Yang Yunxie Wei Zhiqiang Jin Jianghui Xie Ming Peng Wenquan Wang Anping Guo Biyu Xu Jianchun Guo Songbi Chen Liming Ma Mingcheng Wang Yang Zhou Xiaolong Li Ruoxi Li Siyi Guo Xinhui Xiao Zhongqing Wan Feifei An Jie Zhang Qingyun Leng Yin Li Ray Ming Kaimian Li | 2021 | Molecular Plant2021，14，6： | 8 |
| 14 | Disinfection byproduct precursor removal by enhanced coagulation and their distribution in chemical fractions显示文摘Raw water from the Songhua River was treated by four types of coagulants, ferric chloride(FeCl3), aluminum sulfate(Al2(SO4)3),polyaluminum chloride(PACl) and composite polyaluminum(HPAC), in order to remove dissolved organic matter(DOM). Considering the disinfection byproduct(DBP) precursor treatability, DOM was divided into five chemical fractions based on resin adsorption.Trihalomethane formation potential(THMFP) and haloacetic acid formation potential(HAAFP) were measured for each fraction. The results showed that hydrophobic acids(HoA), hydrophilic matter(HiM) and hydrophobic neutral(HoN) were the dominant fractions.Although both HoN and HoA were the main THM precursors, the contribution for THMFP changed after coagulation. Additionally,HoA and HiM were the main HAA precursors, while the contribution of HoN to HAAFP significantly increased after coagulation.HoM was more easily removed than HiM, no matter which coagulant was used, especially under enhanced coagulation conditions.DOC removal was highest for enhanced coagulation using FeCl3 while DBPFP was lowest using PACl. This could indicate that not all DOC fractions contained the precursors of DBPs. Reduction of THMFP and HAAFP by PACl under enhanced coagulation could reach51% and 59% respectively. | Yanmei Zhao Feng Xiao Dongsheng Wang Mingquan Yan Zhe Bi | 2013 | Journal of Environmental Sciences2013，25，11： | 7 |
| 15 | Risk factors of prognosis after acute kidney injury in hospitalized patients显示文摘 | Sasa Nie Zhe Feng Lihua Xia Jiuxu Bai Fenglin Xiao Jian Liu Li Tang Xiangmei Chen | 2017 | Frontiers of Medicine2017，11，3： | 7 |
| 16 | Preparation of amidoxime-based PE/PP fibers for extraction of uranium from aqueous solution显示文摘A novel amidoxime-based fibrous adsorbent,denoted as PE/PP-g-(PAAc-co-PAO), was prepared by preirradiation grafting of acrylic acid and acrylonitrile onto polyethylene-coated polypropylene skin-core(PE/PP)fibers using 60 Co γ-ray irradiation, followed by amidoximation. The original and modified PE/PP fibers were characterized by a series of characterization methods to demonstrate the attachment of amidoxime groups onto the PE/PP fibers. Breaking strength tests confirmed that the fibrous adsorbent could maintain good mechanical properties. The adsorption capacity of the PE/PP-g-(PAAc-coPAO) fibers was investigated in simulated seawater with an initial uranium concentration of 330 μg/L. The uranium adsorption capacity was 2.27 mg/g-adsorbent after 24 h in simulated seawater, and the equilibrium data were well described by the Freundlich isotherm model. The PE/PP-g-(PAAc-co-PAO) adsorbent exhibited good regeneration and recyclability during five adsorption-desorption cycles.The adsorption test was also performed in simulated radioactive effluents with uranium concentrations of 10 and100 μg/L. The effect of the pH value on the adsorption capacity was also studied. At a very low initial concentration 10 μg/L solution, the PE/PP-g-(PAAc-co-PAO)fiber could remove as much as 93.0% of the uranium, and up to 71.2% of the uranium in the simulated radioactive effluent. These results indicated that the PE/PP-g-(PAAcco-PAO) adsorbent could be used in radioactive effluents over a wide range of pH values. Therefore, the PE/PP-g-(PAAc-co-PAO) fibers, with their high uranium selectivity,good regeneration and recyclability,good mechanical properties, and low cost, are promising adsorbents for extracting uranium from aqueous solutions. | Xiao Xu Xiao-Jun Ding Jun-Xuan Ao Rong Li Zhe Xing Xi-Yan Liu Xiao-Jing Guo Guo-Zhong Wu Hong-Juan Ma Xiao-Yan Zhao | 2019 | Nuclear Science and Techniques2019，30，2： | 5 |
| 17 | Research progress of photocatalysis based on highly dispersed titanium in mesoporous SiO_2显示文摘With the development of the human economy and green chemistry, people pay much more attention to environmental safety. Correspondingly, mesoporous TiO_2 and its correlated photocatalysts are able to help people seek for better life. In this review, first of all, we briefly introduce the preparations and applications of mesoporous TiO_2-SiO_2 materials, which exhibit excellent performance in pollutants decomposition and H_2 evolution in photocatalysis. Then, we review the mesoporous composites of TiSiO_2 materials, which are ideal materials used in the photoreduction of air pollutants such as CO_2, NO and NO_2. It is powerfully evident from the literature surveys that these TiO_2 based mesoporous photocatalysts possess a large potential in environment and energy development. | Chencheng Dong Jiahui Ji Zhe Yang Yifei Xiao Mingyang Xing Jinlong Zhang | 2019 | Chinese Chemical Letters2019，30，4： | 5 |
| 18 | Hepatitis B virus X protein promotes human hepatoma cell growth via upregulation of transcription factor AP2α and sphingosine kinase 1显示文摘目的: 鞘氨醇 kinase (SPHK1 ) 1 涉及各种各样的细胞的函数包括房间生长,移植, apoptosis,细胞骨架体系结构和钙 homoeostasis,等等。作为 oncogenic kinase, SPHK1 与癌症的发展和前进被联系。这研究的目的是调查 SPHK1 是否涉及肝炎 B 病毒 X 蛋白质(HBx ) 导致的 hepatocarcinogenesis。 | Zhan-ping LU Ze-lin XIAO Zhe YANG Jiong LI Guo-xing FENG Fu-quan CHEN Ying-hui LI Jin-yan FENG Yu-en GAO Li-hong YE Xiao-dong ZHANG | 2015 | Acta Pharmacologica Sinica2015，36，10： | 5 |
| 19 | Extinction and Positivity for a Doubly Nonlinear Degenerate Parabolic Equation显示文摘这篇论文的目的是为起始的边界价值问题和 u_t = div (|▽ u ~m|^(p-2 )▽ u ~ m ) 的 Cauchy 问题的答案讨论扑灭和确实。弱答案将为 1 是绝灭的,这被证明 < 1 + 1/m 和愿望是的 p ≤为 p 积极 < 为大 t 的 1 + 1/m,在此 m < 0。 | Hong Jun YUAN Song Zhe LIAN Chun Ling CAO Wen Jie GAO Xiao Jing XU | 2007 | Acta Mathematica Sinica,English Series2007，23，10： | 5 |
| 20 | Patient-derived non-small cell lung cancer xenograft mirrors complex tumor heterogeneity显示文摘Objective:Patient-derived xenograft(PDX)models have shown great promise in preclinical and translational applications,but their consistency with primary tumors in phenotypic,genetic,and pharmacodynamic heterogeneity has not been well-studied.This study aimed to establish a PDX repository for non-small cell lung cancer(NSCLC)and to further elucidate whether it could preserve the heterogeneity within and between tumors in patients.Methods:A total of 75 surgically resected NSCLC specimens were implanted into immunodeficient NOD/SCID mice.Based on the successful establishment of the NSCLC PDX model,we compared the expressions of vimentin,Ki67,EGFR,and PD-L1 proteins between cancer tissues and PDX models using hematoxylin and eosin staining and immunohistochemical staining.In addition,we detected whole gene expression profiling between primary tumors and PDX generations.We also performed whole exome sequencing(WES)analysis in 17 first generation xenografts to further assess whether PDXs retained the patient heterogeneities.Finally,paclitaxel,cisplatin,doxorubicin,atezolizumab,afatininb,and AZD4547 were used to evaluate the responses of PDX models to the standard-of-care agents.Results:A large collection of serially transplantable PDX models for NSCLC were successfully developed.The histology and pathological immunohistochemistry of PDX xenografts were consistent with the patients’tumor samples.WES and RNA-seq further confirmed that PDX accurately replicated the molecular heterogeneities of primary tumors.Similar to clinical patients,PDX models responded differentially to the standard-of-care treatment,including chemo-,targeted-and immuno-therapeutics.Conclusions:Our established PDX models of NSCLC faithfully reproduced the molecular,histopathological,and therapeutic characteristics,as well as the corresponding tumor heterogeneities,which provides a clinically relevant platform for drug screening,biomarker discovery,and translational research. | Xuanming Chen Cheng Shen Zhe Wei Rui Zhang Yongsheng Wang Lili Jiang Ke Chen Shuang Qiu Yuanli Zhang Ting Zhang Bin Chen Yanjun Xu Qiyi Feng Jinxing Huang Zhihui Zhong Hongxia Li Guowei Che Kai Xiao | 2021 | Cancer Biology & Medicine2021，18，1： | 5 |
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