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| 1 | Separation and Precise Measurement of Lithium Isotopes in Three Reference Materials Using Multi Collector-Inductively Coupled Plasma Mass Spectrometry显示文摘Lithium separation technique for three reference materials has been established together with precise determination of lithium isotope using a Neptune multi collector-inductively coupled plasma mass spectrometry (MC-ICP-MS). The solutions of lithium element standard reference materials, potassium, calcium, sodium, magnesium and iron single element, were used to evaluate analytical methods applied. Three separate stages of ion-exchange chromatography were carried out using organic cation-exchange resin (AG 50W-X8). Lithium was enriched for the three stages using different eluants, which are 2.8 M HCl, 0.15 M HCl and 0.5 M HCl in 30% ethanol, respectively. The columns for the first and second stages are made of polypropylene, and those for the third stage are made of quartz. Total reagent volume for the entire chemical process was 35 mL for three reference materials. The recovery yielded for the three stages is 98.9-101.2% with an average of 100.0%, 97.6- 101.9% with an average of 99.9%, and 99.8-103.3% with an average of 100.6%, respectively. The precision of this technique is conservatively estimated to be ±0.72-1.04‰ (2σ population), which is similar to the precision obtained by different authors in different laboratories with MC-ICP-MS. The δ 7 Li values ( 7 Li/ 6 Li relative to the IRMM-016 standard) determined for andesite (AGV-2) and basalt (BHVO-2) are 5.68‰ (n=18), 4.33‰ (n=18), respectively. The δ 7 Li value ( 7 Li/ 6 Li relative to the L-SVEC standard) determined for IRMM-016 is -0.01‰ (n=15). All these analytical results are in good agreement with those previously reported. In addition, the results for the same kinds of samples analyzed at the MLR Key Laboratory of Metallogeny and Mineral Assessment, Institute of Mineral Resources, Chinese Academy of Geological Sciences, are consistent with those obtained at the Plasma Laboratory, University of Maryland, within analytical uncertainty. According to these experiment results, it is concluded that this proposed procedure is a suitable method for determining the lithium isotopic composition of natural samples. | TIAN Shihong HOU Zengqian SU Aina HOU Kejun HU Wenjie LI Zhenzhen ZHAO Yue GAO Yanguang LI Yanhe YANG Dan YANG Zhusen | 2012 | Acta Geologica Sinica(English Edition)2012，86，5： | 9 |
| 2 | Dominant mutations in CHK1 cause pronuclear fusion failure and zygote arrest that can be rescued by CHK1 inhibitor显示文摘Dear Editor,Infertility poses a major challenge to human reproductive health,affecting approximately 48 million women worldwide.1 Assisted reproductive techniques(ART),including in vitro fertilization(IVF)and intracytoplasmic sperm injection(ICSI),enable infertile women to have their biological embryos in vitro and further give birth to babies after embryo transfer.However,about 10%of all human embryos produced by ART are blocked in the early embryonic stage2 and approximately 2%fertilized oocytes derived from ART could not accomplish the first cell division.3 About one half of human infertility cases involve an underlying genetic factor,although the majority of genetic causes have remained elusive.4 In this study,we report that dominant mutations in CHK1 are responsible for pronuclear fusion failure and zygote arrest(PFF-ZA)in 7 out of 29 cases,likely through increasing the CHK1 activity.Importantly,PFF-ZA caused by these mutations could be effectively rescued by using the CHK1 inhibitor,PF477736. | Honghui Zhang Tailai Chen Keliang Wu Zhenzhen Hou Shigang Zhao Chuanxin Zhang Yuan Gao Ming Gao Zi-Jiang Chen Han Zhao | 2021 | Cell Research2021，31，7： | 4 |
| 3 | In vitro expansion of human sperm through nuclear transfer显示文摘Dear Editor,Mammalian haploid embryonic stem cells(haESCs)represent an ideal tool for genetic analysis due to the presence of only one set of genetic materials.HaESCs fall into two readily distinguishable groups based on their genome origins:parthenogenetic haESCs(PG-haESCs)that are derived from oocyte-originated parthenogenetic embryos and androgenetic haESCs(AG-haESCs)that are produced through sperm nuclear transfer.Both PG-and AGhaESCs are feasible for delineating genome function at cellular level in vitro.Importantly,AG-haESCs can be used as a sperm replacement and applied to deciphering gene function at organismal level when combined with CRISPR-Cas9 technology.1 However,while PG-haESCs have been generated from both rodent and primates,2-5 AG-haESCs can only be obtained in rodent to date. | Xiaoyu Merlin Zhang Keliang Wu Yuxuan Zheng Han Zhao Junpeng Gao Zhenzhen Hou Meiling Zhang Jiaoyang Liao Jingye Zhang Yuan Gao Yuanyuan Li Lin Li Fuchou Tang Zi-Jiang Chen Jinsong Li | 2020 | Cell Research2020，30，4： | 3 |
| 4 | Polydopamine-mediated graphene oxide and nanohydroxyapatite-incorporated conductive scaffold with an immunomodulatory ability accelerates periodontal bone regeneration in diabetes显示文摘Regenerating periodontal bone tissues in the aggravated inflammatory periodontal microenvironment under diabetic conditions is a great challenge.Here,a polydopamine-mediated graphene oxide(PGO)and hydroxyapatite nanoparticle(PHA)-incorporated conductive alginate/gelatin(AG)scaffold is developed to accelerate periodontal bone regeneration by modulating the diabetic inflammatory microenvironment.PHA confers the scaffold with osteoinductivity and PGO provides a conductive pathway for the scaffold.The conductive scaffold promotes bone regeneration by transferring endogenous electrical signals to cells and activating Ca2+channels.Moreover,the scaffold with polydopamine-mediated nanomaterials has a reactive oxygen species(ROS)-scavenging ability and anti-inflammatory activity.It also exhibits an immunomodulatory ability that suppresses M1 macrophage polarization and activates M2 macrophages to secrete osteogenesis-related cytokines by mediating glycolytic and RhoA/ROCK pathways in macrophages.The scaffold induces excellent bone regeneration in periodontal bone defects of diabetic rats because of the synergistic effects of good conductive,ROS-scavenging,anti-inflammatory,and immunomodulatory abilities.This study provides fundamental insights into the synergistical effects of conductivity,osteoinductivity,and immunomodulatory abilities on bone regeneration and offers a novel strategy to design immunomodulatory biomaterials for treatment of immune-related diseases and tissue regeneration. | Yazhen Li Lu Yang Yue Hou Zhenzhen Zhang Miao Chen Maoxia Wang Jin Liu Jun Wang Zhihe Zhao Chaoming Xie Xiong Lu | 2022 | Bioactive Materials2022，7，12： | 3 |
| 5 | Prediction of VIGS efficiency by the Sfold program and its reliability analysis in Gossypium hirsutum显示文摘Genetic transformation in some plant species,including cotton(Gossypium hirsutum), is hampered by laborious and time-consuming processes and often unachievable. Virus-induced gene silencing(VIGS) by double-stranded RNAs can serve as a reverse-genetics tool to determine gene function. However, knockdown levels vary greatly when using a tobacco rattle virus-based vector that carries different c DNA fragments of a gene. How to choose the optional target fragment for high interference efficiency is very challenging. Addressing this challenge requires increasing the efficacy of small interference RNA(si RNA) in target fragment. Here, we describe a method to assess VIGS efficiency by comparing the following parameters of si RNA in target sequence: the disruption energy of the target(DGdisruption), the differential stability of si RNA duplex ends(DSSE), and the internal stability at positions 9–14 of the si RNA antisense strand(AIS), which are calculated by Sfold program(http://gffzz01a2382fbe924301hw9bx5fbnkxck6f5q.ffgz.tsg.suse.edu.cn). We find that the si RNAs with low DGdisruption, high DSSE and high AIS have high activity and easily result in high VIGS efficiency by experimentally testing the actual knockdown levels of the four target genes, Gh PDS,Gh CLA1, Gh AOS1, and Gh CXE1 via choosing different target sequences for each gene. Therefore, the Sfold program can be used to analyze target sequences when carrying out VIGS design to increase gene-silencing effects in plants. | Xiaoyang Ge Jie Wu Chaojun Zhang Qianhua Wang Yuxia Hou Zuoren Yang Zhaoen Yang Zhenzhen Xu Ye Wang Lili Lu Xueyan Zhang Jinping Hua Fuguang Li | 2016 | Science Bulletin2016，61，7： | 2 |
| 6 | Integrated tool for microsatellite isolation and validation from the reference genome and their application in the study of breeding turnover in an endangered avian population显示文摘Accurate individual identification is required to estimate survival rates in avian populations.For endangered species,non-invasive methods of obtaining individual identification,such as using molted feathers as a source of DNA for microsatellite markers,are preferred because of less disturbance,easy sample preparation and high efficiency.With the availability of many avian genomes,a few pipelines isolating genome-wide microsatellites have been published,but it is still a challenge to isolate microsatellites from the reference genome efficiently.Here,we have developed an integrated tool comprising a bioinformatic pipeline and experimental procedures for microsatellite isolation and validation based on the reference genome.We have identified over 95000 microsatellite loci and established a system comprising 10 highly polymorphic markers(PIC value:0.49–0.93,mean:0.79)for an endangered species,saker falcon(Falco cherrug).These markers(except 1)were successfully amplified in 126 molted feathers,exhibiting high amplification success rates(83.9–99.7%),high quality index(0.90–0.97)and low allelic dropout rates(1–9.5%).To further assess the efficiency of this marker system in a population study,we identified individual sakers using these molted feathers(adult)and 146 plucked feathers(offspring).The use of parent and offspring samples enabled us to infer the genotype of missing samples(N=28),and all adult genotypes were used to ascertain that breeding turnover is a useful proxy for survival estimation in sakers.Our study presents a cost-effective tool for microsatellite isolation based on publicly available reference genomes and demonstrates the power of this tool in estimating key parameters of avian population dynamics. | Xian HOU Pengwei XU Zhenzhen LIN Josephine D’URBAN-JACKSON AndrewDIXON Batbayar BOLD Jiliang XU Xiangjiang ZHAN | 2018 | Integrative Zoology2018，13，5： | 1 |
| 7 | Brocaeloid D,a novel compound isolated from a wheat pathogenic fungus, Microdochium majus 99049显示文摘Microbes serve as the most important resource for drug discovery.During our screening for bioactive compounds from our natural products library,a pathogenic fungus,Microdochium majus strain 99049,from wheat was selected for further investigation.A new alkaloid named brocaeloid D(1),together with six previously characterized compounds(2–7)were identified.Compound 1 belongs to 4-oxoquinoline with C-2 reversed prenylation and a succinimide substructure.All the structures of these newly isolated compounds were determined by different means in spectroscopic experiments.The absolute configurations of 1 was further deduced from comparison of its CD spectrum with that of known compound 2.The bioactivities of these identified compounds were evaluated against several pathogenic microorganisms and cancer cell lines.Compounds 1–5 showed activity against HUH-7 human hepatoma cells with IC50 values of 80μg/mL.Compound 6 showed mild activity against HeLa cells(IC50=51.9μg/mL),weak anti-MTB activity(MIC=80μg/mL),and moderate anti-MRSA activity(MIC=25μg/mL),and compound 7 showed weak anti-MRSA activity(MIC=100μg/mL). | Jingyu Zhang Zhenzhen Wang Zhijun Song Loganathan Karthik Chengjian Hou Guoliang Zhu Lan Jiang Jianying Han Rong Ma Li Li Lixin Zhang Xueting Liu Tom Hsiang | 2019 | Synthetic and Systems Biotechnology2019，4，4： | 1 |
| 8 | Elemene sensitizes pancreatic cancer cells to bortezomib by enhancing proteasome inhibition via molecular patch mechanism显示文摘Dear Editor,Pancreatic cancer*is one of the deadliest cancers,with an overall 5-year survival rate of less than 10%unchanged over the last 40 years.Approximately 80-85%of patients are diagnosed as advanced stage,which are not eligible for curative surgery.1 Therapeutic options for advanced pancreatic cancer patients are still chemotherapy drugs with limited outcomes to improve patient survival and life quality. | Shurong Hou Zhenzhen Li Xiaoling Chen Wenxin Wang Ting Duan Louis Scampavia Yaxia Yuan Timothy P.Spicer Xiabin Chen Tian Xie | 2023 | Signal Transduction and Targeted Therapy2023，8，3： | 1 |
| 9 | 人早期胚胎染色质开放性图谱及其进化的关联显示文摘文章简介发育的进行需要体内的基因能够按照设定的程序、在特定的时间和特定的地点有序地表达,这个过程称为基因表达的编程。人体设定基因表达程序的一种'编程语言'被称为'染色体开放状态'。早期胚胎发育中'染色体开放性'如何指挥人类胚胎的基因表达并不是很清楚。本研究克服研究材料缺乏的难题. | Lei Gao Keliang Wu Zhenbo Liu Xuelong Yao Shenli Yuan Wenrong Tao Lizhi Yi Guanling Yu Zhenzhen Hou Dongdong Fan Yong Tian 刘见桥 陈子江 刘江 | 2019 | 科学新闻2019，0，2： | 1 |
| 10 | Efficient video-frame insertion and deletion detection based on inconsistency of correlations between LBP-coded frames显示文摘 | Zhang Zhenzhen Hou Jianjun Ma Qinglong | 2015 | Security & Communication Networks2015，8，2： | 1 |
| 11 | Performance comparison of different microbial DNA extraction methods on bird feces显示文摘Background:As an important player during food digestion,gut microbiota has attracted much attention in diet adaptation studies in birds.Microbiota extracted from feces has been widely used as a proxy for gut microbiota.Although several methods have been developed for microbial DNA extraction,their performances in the bird feces have not been systematacially evaluated yet.Methods:In this study,we applied three DNA extraction methods(Qiagen,MoBio and Bead)to extract DNA from feces of three avian dietary guilds(granivore,omnivore and carnivore),sequenced V4 region of 16S rRNA gene for each extract and evaluated the performances of DNA yield,DNA integrity,microbial composition,cell lysis capacity and alpha diversity for the three methods on each dietary guild.Results:Bead method was the best on the performance of both DNA yield and DNA integrity regardless of dietary guild.In granivore,microbial relative abundance at both species and phylum levels,alpha diversity and cell lysis capacity were comparable among all methods.In omnivore,Qiagen had the best performance on alpha diversity,fol-lowed by Bead and MoBio.There were small variations on microbial relative abundance at both species and phylum levels among different extraction methods.MoBio exhibited the best performance on cell lysis capacity.In carnivore,considerable variations were found on microbial relative abundance at both species and phylum levels.Qiagen had the best performance on alpha diversity,followed by MoBio and Bead.MoBio had the highest cell lysis capacity.Conclusions:DNA yield and integrity have no obvious impact on microbial composition,alpha diversity or cell lysis capacity.The microbiota results(e.g.,microbial composition,cell lysis capacity,alpha diversity)obtained from differ-ent methods are comparable in granivorous avian species but not in omnivorous or carnivorous birds.Either method could be used in granivore microbiota studies.For omnivores and carnivores,we recommend Qiagen method when the research purpose is microbial diversity and MoBio when gram-positive bacteria is the research target. | Xian Hou Shengkai Pan Zhenzhen Lin Jiliang Xu Xiangjiang Zhan | 2021 | Avian Research2021，12，2： | 1 |
| 12 | Variation in the life history strategy underlies functional diversity of tumors显示文摘Classical r-vs.K-selection theory describes the trade-offs between high reproductive output and competitiveness and guides research in evolutionary ecology.While its impact has waned in the recent past,cancer evolution may rekindle it.Herein,we impose r-or K-selection on cancer cell lines to obtain strongly proliferative r cells and highly competitive K cells to test ideas on life-history strategy evolution.RNA-seq indicates that the trade-offs are associated with distinct expression of genes involved in the cell cycle,adhesion,apoptosis,and contact inhibition.Both empirical observations and simulations based on an ecological competition model show that the trade-off between cell proliferation and competitiveness can evolve adaptively.When the r and K cells are mixed,they exhibit strikingly different spatial and temporal distributions.Due to this niche separation,the fitness of the entire tumor increases.The contrasting selective pressure may operate in a realistic ecological setting of actual tumors. | Tao Li Jialin Liu Jing Feng Zhenzhen Liu Sixue Liu Minjie Zhang Yuezheng Zhang Yali Hou Dafei Wu Chunyan Li Yongbin Chen Hua Chen Xuemei Lu | 2021 | National Science Review2021，8，2： | 0 |
| 13 | Allelic reprogramming of chromatin states in human early embryos显示文摘The reprogramming of parental epigenomes in human early embryos remains elusive.To what extent the characteristics of parental epigenomes are conserved between humans and mice is currently unknown.Here,we mapped parental haploid epigenomes using human parthenogenetic and androgenetic embryos.Human embryos have a larger portion of genome with parentally specific epigenetic states than mouse embryos.The allelic patterns of epigenetic states for orthologous regions are not conserved between humans and mice.Nevertheless,it is conserved that maternal DNA methylation and paternal H3K27me3 are associated with the repression of two alleles in humans and mice.In addition,for DNA-methylation-dependent imprinting,we report 19 novel imprinted genes and their associated germline differentially methylated regions.Unlike in mice,H3K27me3-dependent imprinting is not observed in human early embryos.Collectively,allele-specific epigenomic reprogramming is different in humans and mice. | Shenli Yuan Lei Gao Wenrong Tao Jianhong Zhan Gang Lu Jingye Zhang Chuanxin Zhang Lizhi Yi Zhenbo Liu Zhenzhen Hou Min Dai Han Zhao Zi-Jiang Chen Jiang Liu Keliang Wu | 2024 | National Science Review2024，11，3： | 0 |
| 14 | Exploring Sulfur Chemistry in TMC-1 with NSRT显示文摘There have been several studies on sulfur depletion in dense cores like TMC-1(Taurus Molecular Cloud 1),employing updated reaction networks for sulfur species to explain the missing sulfur in the gas within dense clouds.Most of these studies used a C/O ratio of 0.7 or lower.We present NSRT(NanShan 26m Radio Telescope)observations of TMC-1 alongside results from time-dependent chemical simulations using an updated chemical network.Our findings highlight the impact of the C/O ratio on the gas-phase evolution of C2S and C3S.The simulation results show that the C/O ratio is an important parameter,playing a fundamental role in determining the gas-phase abundances of sulfur species in dense cores. | Wasim Iqbal Xiaohu Li Juan Tuo Ryszard Szczerba Yanan Feng Zhenzhen Miao Jiangchao Yang Jixing Ge Gleb Fedoseev Donghui Quan Qiang Chang Chuan-Lu Yang Tao Yang Gao-Lei Hou Yong Zhang Xuan Fang Xia Zhang Fangfang Li Rong Ma Xiaomin Song Zhiping Kou Yuxuan Sun | 2024 | Chinese Physics Letters2024，41，2： | 0 |
| 15 | Correction to:Performance comparison of different microbial DNA extraction methods on bird feces显示文摘Following publication of the original article(Hou et al.2021),the authors identified an error in Fig.1.The cor-rect figure is given below.The original article(Hou et al.2021)has been updated. | Xian Hou Shengkai Pan Zhenzhen Lin Jiliang Xu Xiangjiang Zhan | 2021 | Avian Research2021，12，2： | 0 |
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