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| 1 | A flow cytometer based protocol for quantitative analysis of bloom-forming cyanobacteria(Microcystis) in lake sediments显示文摘A quantitative protocol for the rapid analysis of Microcystis cells and colonies in lake sediment was developed using a modified flow cytometer,the CytoSense.For cell enumeration,diluted sediment samples containing Microcystis were processed with sonication to disintegrate colonies into single cells.An optimized procedure suggested that 5 mg dw(dry weight)/mL dilution combined with 200 W × 2 min sonication yielded the highest counting efficiency.Under the optimized determination conditions,the quantification limit of this protocol was 3.3×10 4 cells/g dw.For colony analysis,Microcystis were isolated from the sediment by filtration.Colony lengths measured by flow cytometry were similar to those measured by microscopy for the size range of one single cell to almost 400 μm in length.Moreover,the relationship between colony size and cell number was determined for three Microcystis species,including Microcystis flos-aquae,M.aeruginosa and M.wessenbergii.Regression formulas were used to calculate the cell numbers in differentsized colonies.The developed protocol was applied to field sediment samples from Lake Taihu.The results indicated the potential and applicability of flow cytometry as a tool for the rapid analysis of benthic Microcystis.This study provided a new capability for the high frequency monitoring of benthic overwintering and population dynamics of this bloom-forming cyanobacterium. | Quan Zhou Wei Chen Huiyong Zhang Liang Peng Liming Liu Zhiguo Han Neng Wan Lin Li Lirong Song | 2012 | Journal of Environmental Sciences2012，24，9： | 5 |
| 2 | Conductive photo-thermal responsive bifunctional hydrogel system with self-actuating and self-monitoring abilities显示文摘Despite enormous efforts in actuators,most researches are only limited to various actuation behaviors and demonstrations of soft materials.It has not yet been reported to capture and monitor its movement status in an invisible environment.Therefore,it is of great significance to develop a self-sensing and self-actuating dual-function hydrogel actuator system to realize real-time monitoring.Here,we report a bifunctional hydrogel system with self-actuating and self-monitoring abilities,which combines the functions of photothermal actuation and electrical resistance sensing into a single material.The bilayer tough conductive hydrogel synthesized by unconventional complementary concentration recombination and cryogenic freezing technique presents a dense conductive network and high-porosity structure,achieving high toughness at 190.3 kPa of tensile strength,high stretchability(164.3%strain),and the toughness dramatically(1,471.4 kJ·m^(−3)).The working mechanism of the monitoring and self-sensing system is accomplished through the integrated monitoring device of surface temperature–bending angle–electron current,to solve the problem of not apperceiving actuator motion state when encountering obstacles in an invisible environment.We demonstrated for the first time a photothermal actuator’s motion of a football player and goalkeeper to finish the penalty and a soft actuator hand,which can achieve the action of sticking to grab and release under photo-thermal actuation.When connected to the control closed circuit,the actuator realized closed-loop monitoring and sensing feedback.The development of bifunctional hydrogel systems may bring new opportunities and ideas in the fields of material science,circuit technology,sensors,and mechanical engineering. | Neng Chen Yang Zhou Yinping Liu Yuanyuan Mi Sisi Zhao Wang Yang Sai Che Hongchen liu FengJiang Chen Chong Xu Guang Ma Xue Peng Yongfeng Li | 2022 | Nano Research2022，15，8： | 3 |
| 3 | Accumulated Clinical Experiences from Successful Treatment of 1377 Severe and Critically Ill COVID-19 Cases显示文摘Summary:In late December 2019,COVID-19 was firstly recognized in Wuhan,China and spread rapidly to all of the provinces of China.The West Campus of Wuhan Union Hospital,the designated hospital to admit and treat the severe and critically ill COVID-19 cases,has treated a large number of such patients with great success and obtained lots of valuable experiences based on the Chinese guideline(V7.0).To standardize and share the treatment procedures of severe and critically ill cases,Wuhan Union Hospital has established a working group and formulated an operational recommendation,including the monitoring,early warning indicators,and several treatment principles for severe and critically ill cases.The treatment experiences may provide some constructive suggestions for treating the severe and critically ill COVID-19 cases all over the world. | Yong GAO Hai-bo QIU Shu ZHOU Zhen-ning WANG Jian-chu ZHANG Zheng-liang ZHANG Zhao-xin QIAN Hong-bo WANG Shi-huan YU Yi-feng LUO Ya dan WANG Zhuang LIU Wei-ci WANG Ming JIA Li-ming ZHANG Yu ZHANG Yang JIN Yong ZHANG Yong LIU Zi-hua ZHOU Ying SU Hui-qing LI Wei-min XIAO Kai HUANG Ping HE Gang LI Zhao-hui FU Shi LIU Neng xing LIN Fan-jun CHENG | 2020 | Current Medical Science2020，40，4： | 2 |
| 4 | Target alignment in the Shen-Guang Ⅱ Upgrade laser facility显示文摘The Shen-Guang II Upgrade(SG-Ⅱ-U) laser facility consists of eight high-power nanosecond laser beams and one shortpulse picosecond petawatt laser. It is designed for the study of inertial confinement fusion(ICF), especially for conducting fast ignition(FI) research in China and other basic science experiments. To perform FI successfully with hohlraum targets containing a golden cone, the long-pulse beam and cylindrical hohlraum as well as the short-pulse beam and cone target alignment must satisfy tight specifications(30 and 20 μm rms for each case). To explore new ICF ignition targets with six laser entrance holes(LEHs), a rotation sensor was adapted to meet the requirements of a three-dimensional target and correct beam alignment. In this paper, the strategy for aligning the nanosecond beam based on target alignment sensor(TAS) is introduced and improved to meet requirements of the picosecond lasers and the new six LEHs hohlraum targets in the SG-II-U facility. The expected performance of the alignment system is presented, and the alignment error is also discussed. | Lei Ren Ping Shao Dongfeng Zhao Yang Zhou Zhijian Cai Neng Hua Zhaoyang Jiao Lan Xia Zhanfeng Qiao Rong Wu Lailin Ji Dong Liu Lingjie Ju Wei Pan Qiang Li Qiang Ye Mingying Sun Jianqiang Zhu Zunqi Lin | 2018 | High Power Laser Science and Engineering2018，6，1： | 2 |
| 5 | An efficient GPU-based parallel tabu search algorithm for hardware/software co-design显示文摘Hardware/software partitioning is an essential step in hardware/software co-design.For large size problems,it is difficult to consider both solution quality and time.This paper presents an efficient GPU-based parallel tabu search algorithm(GPTS)for HW/SW partitioning.A single GPU kernel of compacting neighborhood is proposed to reduce the amount of GPU global memory accesses theoretically.A kernel fusion strategy is further proposed to reduce the amount of GPU global memory accesses of GPTS.To further minimize the transfer overhead of GPTS between CPU and GPU,an optimized transfer strategy for GPU-based tabu evaluation is proposed,which considers that all the candidates do not satisfy the given constraint.Experiments show that GPTS outperforms state-of-the-art work of tabu search and is competitive with other methods for HW/SW partitioning.The proposed parallelization is significant when considering the ordinary GPU platform. | Neng Hou Fazhi He Yi Zhou Yilin Chen | 2020 | Frontiers of Computer Science2020，14，5： | 2 |
| 6 | Preliminary study on aerosol particle addition calibration method for on-line quantitative analysis of airborne radioactive particles with ICP-MS显示文摘 | Yong Yang Su Zhi Ming Li Hong Bo Dong Guo Qing Zhou Li Hua Zhai Jiang Xu Neng Bin Huang Shi Zeng Feng Rong Zhu | 2011 | International Journal of Environmental Analytical Chemistry2011，，5： | 1 |
| 7 | Impaired hippocampal neurogenesis is involved in cognitive dysfunction induced by thiamine deficiency at early pre-pathological lesion stage显示文摘 | Na Zhao Chunjiu Zhong Yang Wang Yanling Zhao Neng Gong Guomin Zhou Tianle Xu Zhen Hong | 2007 | Neurobiology of Disease2007，，2： | 1 |
| 8 | Theoretical prediction of the Kovat's retention index for oxygen-containing organic compounds using novel topological indices显示文摘 | Fengping Liu Yizeng Liang Chenzhong Cao Neng Zhou | 2007 | Analytica Chimica Acta2007，594，： | 1 |
| 9 | Up - regulation of IL - 10 expression in dendritie cells is involved in Triehosanthin -induced immunosuppression 显示文摘 | Xiaorong Zhou Neng Yang Liming Lu | 2007 | Immunology Letters2007，110，： | 1 |
| 10 | Comparison of high-yield rice in tropical and subtropical environments显示文摘 | Jifeng Ying Shaobing Peng Gaoqun Yang Neng Zhou Romeo M Visperas Kenneth G Cassman | 1998 | Field Crops Research1998，，1： | 1 |
| 11 | Generalization of L-closure spaces显示文摘 | ZHOU WU NENG | 2005 | Fuzzy Sets and Systems2005，149，： | 1 |
| 12 | ( dimethylformamide- O ) copper(Ⅱ)显示文摘 | Ming-Xiong Tan Zhen-Feng Chen Zhou Neng | 2008 | J Acta CrystaUographiea Section E2008，3，64： | 1 |
| 13 | (E)- 2- ( 3,5 - dibromo-2-oxidobenzylideneamino ) -3 - ( 4-hydro xyphenyl ) -propionato-O, N, O' ] ( dimethylformamide- O)eopper(Ⅱ)显示文摘 | Ming-Xiong Tan Zhen-Feng Chen Zhou Neng | 2008 | J Aeta Crystallographica Section E2008，3，64： | 1 |
| 14 | Generalization of L - clousure spaces 显示文摘 | ZHOU Wu - neng | 2005 | Fuzzy Sets and Syestems2005，，149： | 1 |
| 15 | Multiple regression models for energy consumption of office buildings in different climates in China显示文摘 | Siyu ZHOU Neng ZHU | 2013 | Frontiers in Energy2013，7，1： | 1 |
| 16 | 18β-Glycyrrhetinic acid interaction with bovine serum albumin显示文摘 | ZHOU Neng LIANG Yizeng WANG Ping | 2007 | Photochemistry-and Photobiology A: Chemistry2007，185，23： | 1 |
| 17 | Theoretical prediction of the Kovat's retention index for oxygen-containing organic compounds using novel topological indices显示文摘 | Fengping Liu Yizeng Liang Chenzhong Cao Neng Zhou | 2007 | Analytica Chimica Acta2007，594，： | 1 |
| 18 | Generalization of L-closure spaces显示文摘 | Zhou Wu'neng | 2005 | Fuzzy Sets and Systems2005，，149： | 1 |
| 19 | 18-Glycyrrhetinic acid interaction with bovine serum albumin显示文摘 | Yi Zeng Liang Ping Wang | 2007 | Journal of Photochemistry and Photobiology A:Chemistry2007，185，： | 1 |
| 20 | Design and performance of final optics assembly in SG-ⅡUpgrade laser facility显示文摘In high power laser facility for inertial confinement fusion research, final optics assembly(FOA) plays a critical role in the frequency conversion, beam focusing, color separation, beam sampling and debris shielding. The design and performance of FOA in SG-II Upgrade laser facility are mainly introduced here. Due to the limited space and short focal length, a coaxial aspheric wedged focus lens is designed and applied in the FOA configuration. Then the ghost image analysis,the focus characteristic analysis, the B integral control design and the optomechanical design are carried out in the FOA design phase. In order to ensure the FOA performance, two key technologies are developed including measurement and adjustment technique of the wedged focus lens and the stray light management technique based on ground glass.Experimental results show that the design specifications including laser fluence, frequency conversion efficiency and perforation efficiency of the focus spot have been achieved, which meet the requirements of physical experiments well. | Zhaoyang Jiao Ping Shao Dongfeng Zhao Rong Wu Lailin Ji Li Wang Lan Xia Dong Liu Yang Zhou Lingjie Ju Zhijian Cai Qiang Ye Zhanfeng Qiao Neng Hua Qiang Li Wei Pan Lei Ren Mingying Sun Jianqiang Zhu Zunqi Lin | 2018 | High Power Laser Science and Engineering2018，6，2： | 1 |
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