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20篇 您的检索式:作者名="Beibei Luo"
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1小鼠骨骼肌挫伤后基因表达的时间进程(英文)显示文摘Background:Though the mechanisms of skeletal muscle regeneration are deeply understood,those involved in muscle contusion,one of the most common muscle injuries in sports medicine clinics,are not.The objective of this study is to explore the mechanisms involved in muscle regeneration after contusion injury.Methods:In this study,a total of 72 mice were used.Eight of them were randomly chosen for the control group,while the rest were subjected to muscle contusion.Subsequently,their gastrocnemius muscles were harvested at different time points.The changes in muscle morphology were assessed by hematoxylin and eosin(HE) stain.In addition,the gene expression was analyzed by real-time polymerase chain reaction.Results:The data showed that the expression of many genes,i.e.,specific markers of immune cells and satellite cells,regulatory factors for muscle regeneration,cytokines,and chemokines,increased in the early stages of recovery,especially in the first 3 days.Furthermore,there were strict rules in the expression of these genes.However,almost all the genes returned to normal at 14 days post-injury.Conclusion:The sequence of immune cells invaded after muscle contusion was neutrophils,M1 macrophages and M2 macrophages.Some CC(CCL2,CCL3,and CCL4) and CXC(CXCL10) chemokines may be involved in the chemotaxis of these immune cells.HGF may be the primary factor to activate the satellite cells after muscle contusion.Moreover,2 weeks are needed to recover when acute contusion happens as used in this study.Weihua Xiao Yu Liu Beibei Luo Linlin Zhao Xiaoguang Liu Zhigang Zeng Peijie Chen 2016Journal of Sport and Health Science2016,5,1:8
2Cholesterol transport through the peroxisome-ER membrane contacts tethered by PI(4,5)P2 and extended synaptotagmins显示文摘Most mammalian cells take up cholesterol from low-density lipoproteins(LDLs) via receptor-mediated endocytosis.After reaching lysosomes,LDL-derived cholesterol continues to transport to downstream organelles including the ER for specific structural and functional needs.Peroxisomes are recently found to receive cholesterol from lysosomes through lysosomeperoxisome membrane contacts.However,whether and how cholesterol is conveyed from peroxisomes to the ER remain unknown.Here,by combining high-resolution microscopic analyses and in vitro reconstitution of highly purified organelles or artificial liposomes,we demonstrate that peroxisomes form membrane contacts with the ER through the interaction between peroxisomal PI(4,5)P2 and ER-resident extended synaptotagmin-1,2 and 3(E-Syts).Depletion of peroxisomal PI(4,5)P2 or ESyts markedly decreases peroxisome-ER membrane contacts and induces cholesterol accumulation in lysosomes.Furthermore,we show that cholesterol is delivered from 3H-labeled peroxisomes or PI(4,5)P2-containing liposomes to the ER in vitro,and that the presence of peroxisomes augments cholesterol transfer from lysosomes to the ER.Together,our study reveals a new cholesterol transport pathway along the lysosome-peroxisome-ER membrane contacts in the cell.Jian Xiao Jie Luo Ao Hu Ting Xiao Meixin Li Zekai Kong Luyi Jiang Zimu Zhou Yacheng Liao Chang Xie Beibei Chu Honghua Miao Boliang Li Xiongjie Shi Bao-Liang Song 2019Science China(Life Sciences)2019,62,9:8
3Hypertensive stretch regulates endothelial exocytosis of WeibeI-Palade bodies through VEGF receptor 2 signaling pathways显示文摘Weibel-Palade 身体(WPB ) 的调整 endothelial exocytosis,在白血球 trafficking 的第一个阶段,在发炎和损害起一个枢轴的作用。尖锐的机械段仔细与脉管的发炎被联系了,尽管精确机制是未知的。这里,我们证明那高血压的段通过 VEGF 受体 2 调整 endothelial 房间(EC ) 的 WPB 的 exocytosis (VEGFR2 ) 表明小径。段触发一个快速的版本(在分钟以内) 从在有教养的人的 EC 的 WPB 的 von Willebrand 因素和 interleukin-8,通过 P-selectin 的 translocation 把在白血球和 EC 之间的相互作用提升到房间膜。我们进一步证明那高血压的段显著地导致未经触动的 EC 的 P-selectin translocation 并且两个都提高白血球粘附前 vivo 并且在 vivo。导致段的 endothelial exocytosis 经由 VEGFR2/PLCγ 被调停; 1/calcium 小径。有趣地,段也经由一条 VEGFR2/Akt/nitric 氧化物小径导致否定反馈。如此的双效果在颈动脉动脉片断,以及在尖锐高血压的老鼠模型用药理学和基因途径被证实。这些研究为 endothelial exocytosis 作为有势力收缩筋揭示机械段,它被 VEGFR2 发信号调制。因此, VEGFR2 发信号小径可以在限制高血压的段相关的发炎代表新奇治疗学的目标。Yan Xiong Zhenqian Hu Xiaofan Han Beibei Jiang Rongli Zhan Xiaoyu Zhang Yao Lu Chenyang Geng Wei Li Yulong He Yingqing Huo Masabuml Shlbuya Jincai Luo 2013Cell Research2013,23,6:7
4Surface Oxygen Injection in Tin Disulfide Nanosheets for Efficient CO_(2) Electroreduction to Formate and Syngas显示文摘Surface chemistry modification represents a promising strategy to tailor the adsorption and activation of reaction intermediates for enhancing activity.Herein,we designed a surface oxygen-injection strategy to tune the electronic structure of SnS_(2) nanosheets,which showed effectively enhanced electrocatalytic activity and selectivity of CO_(2) reduction to formate and syngas(CO and H_(2)).The oxygen-injection SnS_(2) nanosheets exhibit a remarkable Faradaic efficiency of 91.6%for carbonaceous products with a current density of 24.1 mA cm^(−2) at−0.9 V vs RHE,including 83.2%for formate production and 16.5%for syngas with the CO/H_(2) ratio of 1:1.By operando X-ray absorption spectroscopy,we unravel the in situ surface oxygen doping into the matrix during reaction,thereby optimizing the Sn local electronic states.Operando synchrotron radiation infrared spectroscopy along with theoretical calculations further reveals that the surface oxygen doping facilitated the CO_(2) activation and enhanced the affinity for HCOO*species.This result demonstrates the potential strategy of surface oxygen injection for the rational design of advanced catalysts for CO_(2) electroreduction.Tao Chen Tong Liu Tao Ding Beibei Pang Lan Wang Xiaokang Liu Xinyi Shen Sicong Wang Dan Wu Dong Liu Linlin Cao Qiquan Luo Wei Zhang Wenkun Zhu Tao Yao 2021Nano-Micro Letters2021,13,11:3
5Phonon scattering and exciton localization: molding exciton flux in two dimensional disorder energy landscape显示文摘Two dimensional excitonic devices are of great potential to overcome the dilemma of response time and integration in current generation of electron or/and photon based systems.The ultrashort diffusion length of exciton arising from ultrafast relaxation and low carrier mobility greatly discounts the performance of excitonic devices.Phonon scattering and exciton localization are crucial to understand the modulation of exciton flux in two dimensional disorder energy landscape,which still remain elusive.Here,we report an optimized scheme for exciton diffusion and relaxation dominated by phonon scattering and disorder potentials in WSe2 monolayers.The effective diffusion coefficient is enhanced by>200%at 280 K.The excitons tend to be localized by disorder potentials accompanied by the steadily weakening of phonon scattering when temperature drops to 260 K,and the onset of exciton localization brings forward as decreasing temperature.These findings identify that phonon scattering and disorder potentials are of great importance for long-range exciton diffusion and thermal management in exciton based systems,and lay a firm foundation for the development of functional excitonic devices.Pengfei Qi Yang Luo Beibei Shi Wei Li Donglin Liu Liheng Zheng Zhixin Liu Yanglong Hou Zheyu Fang 2021eLight2021,1,1:3
6Ligand assisted copper-catalyzed Ullmann cross coupling reaction of bromaminic acid with amines显示文摘Three types of ligands have been developed for copper-catalyzed Ullmann cross coupling reaction of bromaminic acid with amines in aqueous solution. Ligands with large steric hindrance and strong electron-donating capacity were beneficial to the reaction. UV–Vis and CV analyses demonstrated that these ligands had strong coordination with copper(I), implying the effect of ligand coordination ability on the stability and catalytic activity of catalytic system.Beibei Shao Hongying Du Xinyu Hao Rongwen Lu Yi Luo Shufen Zhang 2016Chinese Journal of Chemical Engineering2016,24,8:2
7Exercise induces tissue hypoxia and HIF-1α redistribution in the small intestine显示文摘Background'. Exercise induces blood flow redistribution among tissues, leading to splanchnic hypoperfusion. Intestinal epithelial cells are positionedbetween the anaerobic lumen and the highly metabolic lamina propria with an oxygen gradient. Hypoxia-inducible factor (HIF)-la is piv・otal in the transcriptional response to the oxygen flux.Methods: In this study, the pimonidazole hydrochloride staining was applied to observe the tissue hypoxia in different organs, which might beaffected by the blood flow redistribution. The HIF-la luciferase reporter ROSA26 oxygen-dependent degradation domain (ODD)-Luc/+ mousemodel (ODD domain-Luc;female, n = 3—6/group) was used to detect the HIF-la expression in the intestine. We used 3 swimming models: mod・erate exercise for 30 min, heavy-intensity exercise bearing 5% bodyweight for 1.5 h, and long-time exercise for 3 h.Results'. We found that 1 session of swimming at different intensities could induce tissue hypoxia redistribution in the small intestine, colon, liverand kidney, but not in the spleen, heart, and skeletal muscle. Our data showed that exercise exacerbated the extent of physiological hypoxia in thesmall intestine. Next, using ODD-Luc mice, we found that moderate exercise increased the in vivo HIF-la level in the small intestine. The post・exercise HIF-la level was gradually decreased in a time-dependent manner. Interestingly, the redistribution of tissue hypoxia and the increase ofHIF-la expression were not related to the exercise intensity and duration.Conclusion'. This study provided evidence that the small intestine is the primary target organ for exercise-induced tissue hypoxia and HIF-laredistribution, suggesting that HIF-la may be a potential target for the regulation of gastrointestinal functions after exercise.Die Wu Wei Cao Dao Xiang Yi-Ping Hu Beibei Luo Peijie Chen 2020Journal of Sport and Health Science2020,9,1:2
8Proteomic Analysis of High Temperature Stress-Responsive Proteins in Chrysanthemum Leaves显示文摘Chrysanthemum is one of the most important ornamental flowers in the world,and temperature has a significant influence on its field production.In the present study,differentially expressed proteins were investigated in the leaves of Dendranthema grandiflorum‘Jinba’under high temperature stress using label-free quantitative proteomics techniques.The expressed proteins were comparatively identified and analyzed.A total of 1,463 heat-related,differentially expressed proteins were successfully identified by Liquid Chromatography-tandem Mass Spectrometry(LC-MS/MS),and 1,463 heat-related,differentially expressed proteins were successfully identified by mass spectrometry after a high temperature treatment.Among these,701 proteins were upregulated and 762 proteins were downregulated.The in-depth bioinformatics analysis of these differentially expressed proteins revealed that these were involved in energy metabolism pathways,protein metabolism,and heat shock.In the present study,the investigators determined the changes in the levels of some proteins,and their expression at the protein and molecular levels in chrysanthemum to help reveal the mechanism of heat resistance in chrysanthemum.Furthermore,the present study elucidated some of the proteins correlated to heat resistance in chrysanthemum,and their expression changes at the protein and molecular levels to help reveal the mechanism of heat resistance in this flower species.These results provide a theoretical basis for the selection of new heat resistant varieties of chrysanthemum in the field.Xin Li Feiya Liao Qiqi Ma Beibei Jiang Yuanzhi Pan Cheng Luo Xinjie Wang Aining Ran 2021Phyton-International Journal of Experimental Botany2021,90,5:1
9Upcycling of phosphogypsum waste for efficient zinc-ion batteries显示文摘Zinc metal is a promising anode material for next-generation aqueous batteries,but its practical application is limited by the formation of zinc dendrite.To prevent zinc dendrite growth,various Zn^(2+)-conducting but water-isolating solid-electrolyte interphase(SEI)films have been developed,however,the required high-purity chemical materials are extremely expensive.In this work,phosphogypsum(PG),an industrial byproduct produced from the phosphoric acid industry,is employed as a multifunctional protective layer to navigate uniform zinc deposition.Theoretical and experimental results demonstrate that PG-derived CaSO_(4)2H_(2)O can act as an artificial SEI layer to provide fast channels for Zn^(2+)transport.Moreover,CaSO_(4)2H_(2)O could release calcium ions(Ca^(2+))due to its relatively high Kspvalue,which have a higher binding energy than that of Zn^(2+)on the Zn surface,thus preferentially adsorbing to the tips of the protuberances to force zinc ions to nucleate at inert region.As a result,the Zn@PG anode achieves a high Coulombic efficiency of 99.5%during 500 cycles and long-time stability over 1000 hours at 1 m A cm^(-2).Our findings will not only construct a low-cost artificial SEI film for practical metal batteries,but also achieve a high-value utilization of phosphogypsum waste.Huanwen Wang Can Luo Yinyin Qian Caihong Yang Xiaojun Shi Yansheng Gong Rui Wang Beibei He Jun Jin Aidong Tang Edison Huixiang Ang Huaming Yang 2023Journal of Energy Chemistry2023,,6:1
10Controlled synthesis of different morphologies of BaWO 4 crystals via a surfactant-assisted method显示文摘Zhijun Luo Huaming Li Jiexiang Xia Wenshuai Zhu Junxiang Guo Beibei Zhang 2007Journal of Crystal Growth2007,,2:1
11Autonomous reinforcement learning agent for stretchable kirigami design of 2D materials显示文摘Mechanical behavior of 2D materials such as MoS_(2) can be tuned by the ancient art of kirigami.Experiments and atomistic simulations show that 2D materials can be stretched more than 50%by strategic insertion of cuts.However,designing kirigami structures with desired mechanical properties is highly sensitive to the pattern and location of kirigami cuts.We use reinforcement learning(RL)to generate a wide range of highly stretchable MoS_(2) kirigami structures.The RL agent is trained by a small fraction(1.45%)of molecular dynamics simulation data,randomly sampled from a search space of over 4 million candidates for MoS_(2)kirigami structures with 6 cuts.After training,the RL agent not only proposes 6-cut kirigami structures that have stretchability above 45%,but also gains mechanistic insight to propose highly stretchable(above 40%)kirigami structures consisting of 8 and 10 cuts from a search space of billion candidates as zero-shot predictions.Pankaj Rajak Beibei Wang Ken-ichi Nomura Ye Luo Aiichiro Nakano Rajiv Kalia Priya Vashishta 2021npj Computational Materials2021,,1:1
12为期6周的饮食和锻炼干预可改变中国11-13岁肥胖儿童代谢综合症的风险因素(英文)显示文摘目的:研究人员进行了一项随机对照实验,验证为期6周的低卡路里摄入和有氧运动干预能否改变中国青春期前的肥胖儿童代谢综合症(Met S)的风险因素。方法:实验对象被随机分到饮食运动组(实验组)和对照组。实验组的对象根据自身基础代谢率的不同,6周内每天摄取的热量为1 600到2 000卡路里不等,并坚持高负荷有氧运动(每周6天,每天2次,每次3 h)。共215名11-13岁的肥胖儿童参加此实验,其中167人(实验组95人,对照组72人)完成此实验。实验前后测量的参数包括:体重、体质指数、腰围、体脂率、血压及其他采自空腹血样的代谢综合症的相关指标(血清胆固醇、甘油三酯、胰岛素、血糖)。结果:与对照组相比,除却男孩的空腹时血脂(p=0.09),实验组的所有指标均显著下降(p<0.05)。结论:为期6周集中饮食和运动干预,有利于改善中国11-13岁肥胖儿童代谢综合症的风险因素。Beibei Luo Yang Yang David C.Nieman Yajun Zhang Jie Wang Ru Wang Peijie Chen 2013Journal of Sport and Health Science2013,2,4:1
13Hierarchical N-Doped Porous Carbons for Zn–Air Batteries and Supercapacitors显示文摘Nitrogen-doped carbon materials with a large specific surface area,high conductivity,and adjustable microstructures have many prospects for energy-related applications.This is especially true for N-doped nanocarbons used in the electrocatalytic oxygen reduction reaction(ORR)and supercapacitors.Here,we report a low-cost,environmentally friendly,large-scale mechanochemical method of preparing N-doped porous carbons(NPCs)with hierarchical micro-mesopores and a large surface area via ball-milling polymerization followed by pyrolysis.The optimized NPC prepared at 1000°C(NPC-1000)offers excellent ORR activity with an onset potential(Eonset)and half-wave potential(E1/2)of 0.9 and 0.82 V,respectively(vs.a reversible hydrogen electrode),which are only approximately 30 mV lower than that of Pt/C.The rechargeable Zn–air battery assembled using NPC-1000 and the NiFe-layered double hydroxide as bifunctional ORR and oxygen evolution reaction electrodes offered superior cycling stability and comparable discharge performance to RuO2 and Pt/C.Moreover,the supercapacitor electrode equipped with NPC prepared at 800℃ exhibited a high specific capacity(431 F g^−1 at 10 mV s^−1),outstanding rate,performance,and excellent cycling stability in an aqueous 6-M KOH solution.This work demonstrates the potential of the mechanochemical preparation method of porous carbons,which are important for energy conversion and storage.Beibei Guo Ruguang Ma Zichuang Li Shaokui Guo Jun Luo Minghui Yang Qian Liu Tiju Thomas Jiacheng Wang 2020Nano-Micro Letters2020,12,2:1
14Effects of Shengmai Yin(生脉饮)on pulmonary and cardiac function in coronavirus disease 2019 convalescent patients with cardiopulmonary symptoms:a randomized,double blind,multicenter control trial显示文摘OBJECTIVE:To evaluate the efficacy and safety of Shengmai Yin(生脉饮,SMY)on visual analogue scale(VAS)for cardiopulmonary symptoms in coronavirus disease 2019(COVID-19)convalescent patients.METHODS:In this randomized,double blind and multicenter controlled trial,a total of 200 COVID-19 convalescent patients who with cardiopulmonary symptoms were enrolled from three medical centers in Hubei,China.These patients were randomized divided into trial group and the control group,100 patients in each one,SMY and its placebo were applied to respectively,for two weeks.VAS of clinical symptoms included shortness of breath,hidrosis,chest distress,palpitation,and dry cough was performed at 0,1,2 weeks.Decrease in VAS of 30%or more was defined as effective,and a reduction in VAS of 0 was defined as curative.RESULTS:A total of 192 completed the study.The VAS of TCM symptoms showed there was no difference in baseline between the two groups.The VAS in both groups was down-regulated,and there was no significant difference in VAS and cure rates at the first and second week between the two groups.There was no significant difference in breath,hidrosis,palpitation,and dry cough between the two groups but SMY treatment for two weeks has remarkable therapeutic effects in chest distress than placebo.CONCLUSIONS:SMY could effectively ameliorate the symptoms of chest distress,and improve the quality of life of the COVID-19 convalescent patients.AN Xuedong MAO Lina XIA Ping SU Wen WANG Beibei KOU Leiya ZHANG Zequan QI Meng HU Song CHEN Jing LI Xiujuan LIU Jinwei ZHOU Juan QIAO Jie LUO Dan LUO Guangwei YAN Youqin YANG Guiping DONG Dandan ZHOU Wei TAO Junxiu JIN De TONG Xiaolin WEI Li 2023Journal of Traditional Chinese Medicine2023,43,1:1
15Lysosomal cholesterol accumulation is commonly found in most peroxisomal disorders and reversed by 2-hydroxypropyl-β-cyclodextrin显示文摘Peroxisomal disorders(PDs)are a heterogenous group of diseases caused by defects in peroxisome biogenesis or functions.Xlinked adrenoleukodystrophy is the most prevalent form of PDs and results from mutations in the ABCD1 gene,which encodes a transporter mediating the uptake of very long-chain fatty acids(VLCFAs).The curative approaches for PDs are very limited.Here,we investigated whether cholesterol accumulation in the lysosomes is a biochemical feature shared by a broad spectrum of PDs.We individually knocked down fifteen PD-associated genes in cultured cells and found ten induced cholesterol accumulation in the lysosome.2-Hydroxypropyl-β-cyclodextrin(HPCD)effectively alleviated the cholesterol accumulation phenotype in PD-mimicking cells through reducing intracellular cholesterol content as well as promoting cholesterol redistribution to other cellular membranes.In ABCD1 knockdown cells,HPCD treatment lowered reactive oxygen species and VLCFA to normal levels.In Abcd1 knockout mice,HPCD injections reduced cholesterol and VLCFA sequestration in the brain and adrenal cortex.The plasma levels of adrenocortical hormones were increased and the behavioral abnormalities were greatly ameliorated upon HPCD administration.Together,our results suggest that defective cholesterol transport underlies most,if not all,PDs,and that HPCD can serve as a novel and effective strategy for the treatment of PDs.Lewei Dong Jian Xiao Shuai Liu Gang Deng Yacheng Liao Beibei Chu Xiaolu Zhao Bao-Liang Song Jie Luo 2023Science China(Life Sciences)2023,66,8:0
16Future of targeted therapy for gastrointestinal cancer:Claudin 18.2显示文摘The treatment of gastrointestinal cancer has always been a crucial research area,and targeted therapy has been receiving increasing attention.At present,the effect of targeted therapy is unsatisfactory for gastric cancer.Thus,the discovery of new targets is crucial.Claudin 18.2(CLDN18.2),a member of the claudin family,belongs to the tight junction protein family that controls the flow of molecules between cell layers.CLDN18.2 expression has been discussed in many studies.In recent years,there have been many studies on targeted therapy with CLDN18.2-ideal monoclonal antibody 362.Furthermore,CLDN18.2-specific chimeric antigen receptor T therapy has been used for CLDN18.2-positive tumors,such as gastric and pancreatic cancers.Considerable research has been focused on CLDN18.2.CLDN18.2,a newly discovered marker for precise targeted therapy of gastric cancer,could offer new hope for the treatment of gastric cancer.Qian Niu Jiamin Liu Xiaoxiao Luo Beibei Su Xianglin Yuan 2021Oncology and Translational Medicine2021,7,3:0
17Study on the Distribution of Migrant Workers' Employment Destinations A Case Study of Henan Province, China显示文摘Gao Genghe Luo Qing Hai Beibei Xu Jiawei Li Erling 2015Journal of Earth Science and Engineering2015,5,1:0
18Research of Inclusion Complexation of 4,4'-Thiodiphenol Effected by β-Cyclodextrin in Aqueous Solutions and the Effect on Photodegradation显示文摘The inclusion behavior of 4,4′ -Thiodiphenol (TDP),a typical bisphenol and endocrine disruptor,reacts with β-cyclodextrin (β-CD) in aqueous solutions has been investigated by means of UV absorption spectrum and quantum-chemical calculation with Gaussian 98 software. The results show that the inclusion behavior of TDP is quite different in acidic solutions (pH 5.9) from that in alkaline solutions (pH 10.0). This behavior difference is attributed to the different formula structures in aqueous solutions at acidic and alkaline pH values that are demonstrated by quantumchemical modeling and calculation. TDP forms a 1∶1 β-CD inclusion complex in aqueous solutions. The equilibrium constant K was calculated to be 553.49 L/mol at pH 5.9 and 1 318.20 L/mol at pH 10.0 respectively for the inclusion complex reaction by using the modified Benesi-Heldbrand equation. After inclusion TDP's structure is changed especially at the inclusion part with the bond order becoming larger,which results in inhibitive photodegradation during direct photooxidation and H2O2 assisted photooxidation.ZHANG Xu WU Feng WANG Beibei DENG Nansheng LUO Mingdao 2007Wuhan University Journal of Natural Sciences2007,12,3:0
19Stabilizing and structuring oil–oil interfaces by molecular brush surfactants显示文摘Oil-in-oil emulsions provide a unique platform for numerous applications not compatible with water or aqueous systems.However,the limited types and complicated synthesis process of surfactants are problematic.Here,we put forward a simple,yet powerful strategy to stabilize the oil–oil interface,then prepare oil-in-oil emulsions,by using the co-assembly of polar molecular brushes and nonpolar oligomeric ligands at the DMF–octane interface.Molecular brush surfactants(MBSs)form and assemble in situ at the interface,minimizing the interfacial energy and producing a robust film.We find that the binding energy of MBS to the interface is sufficiently high,leading to the construction of structured liquids by the interfacial jamming of MBSs.Using MBSs as emulsifiers,different types of emulsions including DMF-inoctane,octane-in-DMF,and high internal phase emulsions can be easily prepared,showing promising applications in microreactor systems and advanced composite materials.Beibei Wang Zhao Zhang Weixiao Feng Jiaqiu Luo Shaowei Shi 2022Aggregate2022,3,6:0
20Proteomic Analysis of Chrysanthemum Lateral Buds after Removing Apical Dominance Based on Label-Free Technology显示文摘Studying the genetic basis and regulatory mechanism of chrysanthemum lateral bud outgrowth is of great significance for reduction the production cost of cut chrysanthemum.To clarify the molecular basis of lateral bud elongation after removal of apical dominance in chrysanthemum,label-free quantification analysis was used to analyze the proteome changes after apical bud removal.Quantitative real-time PCR(qPCR)was used to analyze the changes in the expression of three plant hormone-related genes.A total of 440 differentially expressed proteins were successfully identified at three time points during the lateral bud elongation.The number of differentially expressed proteins in the three stages(24 h/0 h,48 h/0 h,48 h/24 h)were 219,332,and 97,respectively.The difference in expressed proteins in the three comparison stages mainly involves RNA processing and modification;translation,ribosomal structure and biogenesis;Posttranslational modification,protein turnover,and chaperones.Path analysis showed that there was various physiological activities in the process of lateral bud dormancy breaking and elongation,which involved energy metabolism,biosynthesis,signal transduction and stress response in the growth process of lateral buds.qPCR indicated that the expression of cytokinin synthesis related gene was significantly increased after the removal of apical dominance,while the expression of strigolactones synthesis related gene experiences a dramatic fall to promote the development of the lateral buds.However,there was a drop before a slight increase in the expression of the auxin synthesis related gene,which was mainly due to the removal of apical dominance that led to the loss of indoleacetic acid in the main stem.However,with formation of the new apical source,indoleacetic acid can be released again.Sicong Zheng Jingjing Song Cheng Luo Xin Li Qiqi Ma Beibei Jiang Qinglin Liu Yuanzhi Pan 2022Phyton-International Journal of Experimental Botany2022,91,3:0
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