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| 1 | Allele-defined genome reveals biallelic differentiation during cassava evolution显示文摘Dear Editor,Generation of heterozygous genomes by hybridization between or within species can help maintain plant diversity and serve as a potential source of new species(Baek et al.,2018).Moreover,genomic heterozygosity is associated with genomic coadaptation,developmental stability,and heterosis.Accurate definition of alleles in haplotypes is necessary to precisely characterize allelic variation controlling agriculturally important traits(Shi et al.,2019).Currently,most released genomes have mosaic assembly of haplotypes due to random selection or collapse of alleles during genome assembly(Shi et al.,2019),which masked allelic variation and functional differentiation of divergent alleles in heterozygous species. | Wei Hu Changmian Ji Haitao shi Zhe Liang Zehong Ding Jianqiu Ye Wenjun Ou Gang Zhou Weiwei Tie Yan Yan Jinghao Yang Xiaoying Yang Yunxie Wei Zhiqiang Jin Jianghui Xie Ming Peng Wenquan Wang Anping Guo Biyu Xu Jianchun Guo Songbi Chen Liming Ma Mingcheng Wang Yang Zhou Xiaolong Li Ruoxi Li Siyi Guo Xinhui Xiao Zhongqing Wan Feifei An Jie Zhang Qingyun Leng Yin Li Ray Ming Kaimian Li | 2021 | Molecular Plant2021，14，6： | 8 |
| 2 | Influences of sample storage and grinding on the extraction of soil amino sugars显示文摘Soil amino sugars have been widely used to evaluate the potential roles of microbes in mediating soil carbon(C)cycling and various pretreatment methods were used for its extraction.However,few studies assessed their potential influences on the soil amino sugar extraction.In this study,we investigated the effects of sample storage method and grinding on amino sugar extraction across different climatic zones and land uses.Results showed that the concentrations of soil amino sugars varied greatly among sample pretreatments and their impacts were highly dependent on climatic condition and land use.Specifically,higher concentrations of amino sugars were extracted from field-moist samples than dried samples in subtropical grassland,temperate forest and arable land with no significant differences among storage methods for the samples from subtropical forest,arable land,and temperate grassland.Moreover,grinding improved the extraction efficiency of amino sugars for the dried soils.Due to the reduced extraction concentration in dried soils,field-moist samples were recommended in priority.For the dried soils used for the long-term storage,grinding can be an option to improve the extraction efficiency.Such information will be valuable for reducing the uncertainty and improving the accuracy during the determination of soil amino sugars. | Zhijian Mou Luhui Kuang Biyue Yan Xinyu Zhang Yunqiang Wang Zhanfeng Liu | 2020 | Soil Ecology Letters2020，2，2： | 1 |
| 3 | Enrichment,expansion,and identification of stem-like cells from hepatic carcinoma HepG2 cells显示文摘Objective:To establish a method for enriching,culturing and identifying stem-like subpopulation from human hepatic carcinoma cells,and to explore its biological properties.Methods:HepG2cells were cultured in cancer stem cell(CSC)medium to form spheroids.The stem-like HepG2cells obtained from tumor spheroids were then expanded.Flow cytometry was used to detect the expression of CD90 and CD133on the surface of stem-like HepG2 cells.The in vitro colony forming ability and in vivo tumorigenicity were detected by clone formation assay and tumorigenesis assay.Results:HepG2cells could grow in suspension and form spheroids in CSC medium.The stem-like cells had the ability of self-renewal and proliferation.The expression of CD90and CD133on the surface of these stem-like cells were higher than those of parental HepG2cells(P<0.01).The colony formation ability of stem-like cells was higher than that of parental HepG2cells.When injected with 1×106 cells,stemlike cells could form tumors earlier than parental cells in nude mice.The stem-like cells’tumorigenesis rate was higher and the tumor size was larger than those of parental HepG2cells(P<0.01).Conclusion:The suspension sphere culture method could enrich stem-like cells from HepG2cells.The obtained stem-like cells possessed the properties of self-renewal in vitro and tumorigenicity in vivo. | Biyu Cui Yebing Pang Xuehui Zhang Jing Lan Xilei Li Manya Wu Xing Guo Rong Liang Yan Feng Xiaoling Luo | 2017 | 广西医科大学学报2017，34，1： | 0 |
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