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| 1 | Prevalence of Autism Spectrum Disorder in China:A Nationwide Multi-center Population-based Study Among Children Aged 6 to 12 Years显示文摘This study aimed to obtain the first national estimate of the prevalence of autism spectrum disorder(ASD) in Chinese children.We targeted the population of 6 to 12-year-old children for this prevalence study by multistage convenient cluster sampling.The Modified Chinese Autism Spectrum Rating Scale was used for the screening process.Of the target population of 142,086 children,88.5%(n=125,806) participated in the study.A total of 363 children were confirmed as having ASD.The observed ASD prevalence rate was 0.29%(95% CI:0.26%-0.32%) for the overall population.After adjustment for response rates,the estimated number of ASD cases was867 in the target population sample,thereby achieving an estimated prevalence of 0.70%(95% CI:0.64%-0.74%).The prevalence was significantly higher in boys than in girls(0.95%;95% CI:0.87%-1.02% versus 0.30%;95%CI:0.26%-0.34%;P <0.001).Of the 363 confirmed ASD cases,43.3% were newly diagnosed,and most of those(90.4%) were attending regular schools,and 68.8% of the children with ASD had at least one neuropsychiatric comorbidity.Our findings provide reliable data on the estimated ASD prevalence and comorbidities in Chinese children. | Hao Zhou Xiu Xu Weili Yan Xiaobing Zou Lijie Wu Xuerong Luo Tingyu Li Yi Huang Hongyan Guan Xiang Chen Meng Mao Kun Xia Lan Zhang Erzhen Li Xiaoling Ge Lili Zhang Chunpei Li Xudong Zhang Yuanfeng Zhou Ding Ding Andy Shih Eric Fombonne Yi Zheng Jisheng Han Zhongsheng Sun Yong-hui Jiang Yi Wang LATENT-NHC Study Team | 2020 | Neuroscience Bulletin2020，36，9： | 158 |
| 2 | A life support-based comprehensive treatment regimen dramatically lowers the in-hospital mortality of patients with fulminant myocarditis: a multiple center study显示文摘Fulminant myocarditis(FM) has unacceptable high mortality. This study aimed to evaluate the therapeutic efficacy of a life support-based comprehensive treatment regimen(LSBCTR), a completely novel treatment regimen, for FM. A total of 169 FM patients recruited from January 2008 to December 2018 were divided into two groups: patients receiving LSBCTR(81 cases),which includes(i) mechanical life support(positive pressure respiration, intra-aortic balloon pump with or without extracorporeal membrane oxygenation),(ii) immunomodulation therapy using sufficient doses of glucocorticoids and immunoglobulins, and(iii) application of neuraminidase inhibitors, and those receiving conventional treatment(88 cases). The endpoints were in-hospital death and heart-transplantation. Of all the population, 44 patients(26.0%) died in hospitals. Inhospital mortality was 3.7%(3/81) for LSBCTR group and 46.6%(41/88) for traditional treatment(P<0.001). Early application of LSBCTR, mechanical life support, neuraminidase inhibitors, and immunomodulation therapy significantly contributed to reduction in in-hospital mortality. This study describes a novel treatment regimen for FM patients that dramatically reduces inhospital mortality. Its generalization and clinical application will efficiently save lives although further optimization is needed.This study offers an insight that virus infection induced inflammatory waterfall results in cardiac injury and cardiogenic shock and is the therapeutic target. | Sheng Li Shengyong Xu Chenze Li Xiao Ran Guanglin Cui Mengying He Kun Miao Chunxia Zhao Jiangtao Yan Rutai Hui Ning Zhou Yan Wang Jiangang Jiang Jing Zhang Daowen Wang | 2019 | Science China(Life Sciences)2019，62，3： | 43 |
| 3 | Present and future of prophylactic antibiotics for severe acute pancreatitis显示文摘AIM: To investigate the role of prophylactic antibiotics in the reduction of mortality of severe acute pancreatitis (SAP) patients, which is highly questioned by more and more randomized controlled trials (RCTs) and metaanalyses. METHODS: An updated meta-analysis was performed. RCTs comparing prophylactic antibiotics for SAP with control or placebo were included for meta-analysis. The mortality outcomes were pooled for estimation, and re-pooled estimation was performed by the sensitivity analysis of an ideal large-scale RCT. RESULTS: Currently available 11 RCTs were included. Subgroup analysis showed that there was significant reduction of mortality rate in the period before 2000, while no significant reduction in the period from 2000 [Risk Ratio, (RR ) = 1.01, P = 0.98]. Funnel plot indi-cated that there might be apparent publication bias in the period before 2000. Sensitivity analysis showed that the RR of mortality rate ranged from 0.77 to 1.00 with a relatively narrow confidence interval (P < 0.05). However, the number needed to treat having a minor lower limit of the range (7-5096 patients) implied that certain SAP patients could still potentially prevent death by antibiotic prophylaxis. CONCLUSION: Current evidences do not support prophylactic antibiotics as a routine treatment for SAP, but the potentially benefited sub-population requires further investigations. | Kun Jiang Wei Huang Xiao-Nan Yang Qing xia | 2012 | World Journal of Gastroenterology2012，18，3： | 37 |
| 4 | Intelligent and connected vehicles: Current status and future perspectives显示文摘Intelligent connected vehicles(ICVs) are believed to change people's life in the near future by making the transportation safer,cleaner and more comfortable. Although many prototypes of ICVs have been developed to prove the concept of autonomous driving and the feasibility of improving traffic efficiency, there still exists a significant gap before achieving mass production of high-level ICVs. The objective of this study is to present an overview of both the state of the art and future perspectives of key technologies that are needed for future ICVs. It is a challenging task to review all related works and predict their future perspectives, especially for such a complex and interdisciplinary area of research. This article is organized to overview the ICV key technologies by answering three questions: what are the milestones in the history of ICVs; what are the electronic components needed for building an ICV platform; and what are the essential algorithms to enable intelligent driving? To answer the first question, the article has reviewed the history and the development milestones of ICVs. For the second question, the recent technology advances in electrical/electronic architecture, sensors, and actuators are presented. For the third question, the article focuses on the algorithms in decision making, as the perception and control algorithm are covered in the development of sensors and actuators. To achieve correct decision-making, there exist two different approaches: the principle-based approach and data-driven approach. The advantages and limitations of both approaches are explained and analyzed. Currently automotive engineers are concerned more with the vehicle platform technology, whereas the academic researchers prefer to focus on theoretical algorithms. However, only by incorporating elements from both worlds can we accelerate the production of high-level ICVs. | YANG DianGe JIANG Kun ZHAO Ding YU ChunLei CAO Zhong XIE ShiChao XIAO ZhongYang JIAO XinYu WANG SiJia ZHANG Kai | 2018 | Science China(Technological Sciences)2018，61，10： | 31 |
| 5 | Electro-acupuncture regulates the cholinergic anti-inflammatory pathway in a rat model of chronic obstructive pulmonary disease显示文摘Objective: Acupuncture has a definite therapeutic effect on chronic obstructive pulmonary disease(COPD), and the cholinergic anti-inflammatory pathway(CAP) has been shown to be involved in regulation of inflammation. In this study, we investigated whether electro-acupuncture(EA) affects the CAP in COPD.Methods: Sprague-Dawley rats were induced into COPD through exposure to cigarette smoke combined with lipopolysaccharide. EA treatment was applied at Zusanli(ST36) and Feishu(BL13) points for 30 min/d for 7 d. Seventy-two rats were randomly divided into six study groups, including normal, normal + EA,normal + α-bungarotoxin(a-BGT)(the antagonist of the nicotinic acetylcholine receptor a7 subunit(a7 nAChR)) + EA, COPD, COPD + EA, and COPD + α-BGT + EA. Lung function, pathology and vagus nerve discharge were tested. The levels of acetylcholine(ACh), acetylcholinesterase(AChE), interleukin-6(IL-6) and tumor necrosis factor-a(TNF-a) in bronchoalveolar lavage fluid(BALF) and lung tissue were measured by enzyme-linked immunosorbent assay. The mRNA and protein expression and immunoreactivity of a7 nAChR and its postreceptor inflammation signal pathway, including janus kinase 2(JAK2), signal transducers and activators of transcription 3(STAT3), nuclear factor-KB(NF-κB), were observed by quantitative reverse transcription-polymerase chain reaction, Western blot and immunohistochemistry.Results: Compared with normal rats, there were a significant decline in lung function and discharge of the vagus nerve(P < 0.01), a marked sign of lung inflammation and an increase of ACh, AChE, IL-6 and TNF-a level in BALF or lung tissue(P < 0.05, P < 0.01) and higher expression of a7 nAChR, JAK2, STAT3 and NF-κB(P<0.05, P<0.01) in the COPD rats. In rats receiving EA, the lung function and vagal discharge were enhanced(P < 0.01), lung inflammation was improved and the levels of ACh, AChE, IL-6 and TNF-a were decreased(P<0.01). Further, the expression of a7 nAChR, JAK2, STAT3 and NF-κB was downregulated(P < 0.05, P < 0.01). However, the above effects of EA were blocked in rats injected with α-BGT(P < 0.01).Conclusion: EA treatment can reduce the lung inflammatory response and improve lung function in COPD, which may be related to its involvement in the regulation of CAP. | Xin-fang Zhang Shui-ying Xiang Wen-ye Geng Wen-juan Cong Jing Lu Chuan-wei Jiang Kun Wang Zi-bing Liu | 2018 | Journal of Integrative Medicine2018，16，6： | 32 |
| 6 | Stenting of iliac vein obstruction following catheter-directed thrombolysis in lower extremity deep vein thrombosis显示文摘 | MENG Qing-YOU LI Xiao-qiang JIANG Kun QIAN Ai-min SANG Hong-fei RONG Jian-jie DUAN Peng-fei ZHU Li-wei | 2013 | Chinese Medical Journal2013，，18： | 22 |
| 7 | A novel gene delivery system targeting cells expressing VEGF receptors显示文摘Two ligand oligopeptides GV1 and GV2 were designed according to the putative binding region of VEGF to its receptors. GV1, GV2 and endosome releasing oligopeptide HA20 were conjugated with poly-L-lysine or protamine and the resulting conjugates could interact with DNA in a noncovalent bond to form a complex. Using pSV2-β-galactosidase as a reporter gene, it has been demonstrated that exogenous gene was transferred into bovine aortic arch-derived endothelial cells (ABAE) andhuman malignant melanoma cell lines (A375) in vitro. In vivo experiments, exogenous gene was transferred into tumor vascular endothelial cells and tumor cells of subcutaneously transplanted human colon cancer LOVO, human malignant melanoma A375 and human hepatoma graft in nude mice. This system could also target gene to intrahepatically transplanted human hepatoma injected via portal vein in nude mice. These results are correlated with theGene delivery system targeting VEGF receptors relevant receptors (flt-1, flk-1/KDR) expression on the targeted cells and tissues. | LI JUN MIN JUN SONG HAN YI HUANG PEI KUN TIAN SHU MIN QU MIN YAO HUI QIU JIANG DA FANG WAN JING CHU LUO CHENG XIAO GU JIAN REN GU( National Labomtory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032,China)(National Laboratory of | 1999 | Cell Research1999，9，1： | 22 |
| 8 | Early nasogastric enteral nutrition for severe acute pancreatitis: A systematic review显示文摘AIM: To evaluate the effectiveness and safety of early nasogastric enteral nutrition (NGEN) for patients with severe acute pancreatitis (SAP). METHODS: We searched Cochrane Central Register of Controlled Trials (Issue 2, 2006), Pub-Medline (1966-2006), and references from relevant articles. We included randomized controlled trials (RCTs) only, which reported the mortality of SAP patients at least. Two reviewers assessed the quality of each trial and collected data independently. The Cochrane Collaboration’s RevMan 4.2.9 software was used for statistical analysis. RESULTS: Three RCTs were included, involving 131 patients. The baselines of each trial were comparable. Meta-analysis showed no significant differences in mortality rate of SAP patients between nasogastric and conventional routes (RR = 0.76, 95% CI = 0.37 and 1.55, P = 0.45), and in other outcomes, including time of hospital stay (weighted mean difference = -5.87, 95% CI = -20.58 and 8.84, P = 0.43), complication rate of infection (RR = 1.41, 95% CI = 0.62 and 3.23, P = 0.41) or multiple organ defi ciency syndrome (RR = 0.97, 95% CI = 0.27 and 3.47, P = 0.97), rate of admission to ICU (RR = 1.00, 95% CI = 0.48 and 2.09, P = 0.99) or conversion to surgery (RR = 0.66, 95% CI = 0.12 and 3.69, P = 0.64), as well as recurrence of re-feeding pain and adverse events associated with nutrition. CONCLUSION: Early NGEN is a breakthrough in the management of SAP. Based on current studies, early NGEN appears effective and safe. Since the available evidence is poor in quantity, it is hard to make an accurate evaluation of the role of early NGEN in SAP.Before recommendation to clinical practice, further high qualified, large scale, randomized controlled trials are needed. | Kun Jiang Xin-Zu Chen Qing Xia Wen-Fu Tang Lei Wang | 2007 | World Journal of Gastroenterology2007，13，39： | 21 |
| 9 | A step metallogenetic model for gold deposits in the northwestern Shandong Peninsula, China显示文摘Based on our study of the nature and variation of mineralization-controlling faults in their deep extension and the spatial distribution of gold orebodies in the northwestern Shandong Peninsula (NSP), here we propose that the gold deposits in the Jiaojia gold belt are controlled by listric faults and the gold deposits in the NSP are controlled by extensional fault system. We also suggest that there is a multiple mineralization space in the deep part of the NSP gold belt. As steps or benches occur along the deep extention of the listric fault where its dipping angle changes from steep to gentle, a number of gold deposits are correspondingly distributed as a steplike pattern, which is named as a step metallogenetic model for gold deposits in the NSP. The gold deposits in Shandong Peninsula were formed in the Cretaceous when the crust in the region experienced extention and thinning following the strong collision between North China Plate and Yangtze Plate during the Triassic. Crust anataxis, fluid remobilization, and extention-detachement of the crust are the leading factors for the gold mineralization. | SONG MingChun YI PiHou XU JunXiang CUI ShuXue SHEN Kun JIANG HongLi YUAN WenHua WANG HuaJiang | 2012 | Science China Earth Sciences2012，55，6： | 21 |
| 10 | Surface settlement caused by twin-parallel shield tunnelling in sandy cobble strata显示文摘City metro tunnels are usually constructed as twin-parallel tunnels and their adjacent construction may lead to surface deformation,affecting the surface environment and the safety of the tunnels.Due to its strong dispersion,sandy cobble strata can be easily disturbed by shield tunneling.Based on the project of the Chengdu Metro Line 1,field and model tests were carried out to study the surface settlement caused by shield tunneling in sandy cobble strata by measuring surface settlement curves,ground loss ratios and construction influence zones.The discrete element method(DEM) was used to study the factors affecting the formation of ground arches in sandy cobble strata at the microscopic level.Results show that the shape of the surface settlement curve in sandy cobble strata is different from that in soft soil.The buried depth and clear spacing of the two tunnels had a significant impact on the formation of ground arches. | Chuan HE Kun FENG Yong FANG Ying-chao JIANG | 2012 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2012，13，11： | 21 |
| 11 | Virtual target guidance-based distributed model predictive control for formation control of multiple UAVs显示文摘The paper proposes a Virtual Target Guidance(VTG)-based distributed Model Predictive Control(MPC) scheme for formation control of multiple Unmanned Aerial Vehicles(UAVs).First, a framework of distributed MPC scheme is designed in which each UAV only shares the information with its neighbors, and the obtained local Finite-Horizon Optimal Control Problem(FHOCP) can be solved by swarm intelligent optimization algorithm.Then, a VTG approach is developed and integrated into the distributed MPC scheme to achieve trajectory tracking and obstacle avoidance.Further, an event-triggered mechanism is proposed to reduce the computational burden for UAV formation control, which takes into consideration the predictive state errors as well as the convergence of cost function.Numerical simulations show that the proposed VTG-based distributed MPC scheme is more computationally efficient to achieve formation control of multiple UAVs in comparison with the traditional distributed MPC method. | Zhihao CAI Longhong WANG Jiang ZHAO Kun WU Yingxun WANG | 2020 | Chinese Journal of Aeronautics2020，33，3： | 20 |
| 12 | Lung transplantation as therapeutic option in acute respiratory distress syndrome for coronavirus disease 2019-related pulmonary fibrosis显示文摘Background:Critical patients with the coronavirus disease 2019(COVID-19),even those whose nucleic acid test results had turned negative and those receiving maximal medical support,have been noted to progress to irreversible fatal respiratory failure.Lung transplantation(LT)as the sole therapy for end-stage pulmonary fibrosis related to acute respiratory distress syndrome has been considered as the ultimate rescue therapy for these patients.Methods:From February 10 to March 10,2020,three male patients were urgently assessed and listed for transplantation.After conducting a full ethical review and after obtaining assent from the family of the patients,we performed three LT procedures for COVID-19 patients with illness durations of more than one month and extremely high sequential organ failure assessment scores.Results:Two of the three recipients survived post-LT and started participating in a rehabilitation program.Pearls of the LT team collaboration and perioperative logistics were summarized and continually improved.The pathological results of the explanted lungs were concordant with the critical clinical manifestation,and provided insight towards better understanding of the disease.Government health affair systems,virology detection tools,and modern communication technology all play key roles towards the survival of the patients and their rehabilitation.Conclusions:LT can be performed in end-stage patients with respiratory failure due to COVID-19-related pulmonary fibrosis.If confirmed positive-turned-negative virology status without organ dysfunction that could contraindicate LT,LT provided the final option for these patients to avoid certain death,with proper protection of transplant surgeons and medical staffs.By ensuring instant seamless care for both patients and medical teams,the goal of reducing the mortality rate and salvaging the lives of patients with COVID-19 can be attained. | Jing-Yu Chen Kun Qiao Feng Liu Bo Wu Xin Xu Guo-Qing Jiao Rong-Guo Lu Hui-Xing Li Jin Zhao Jian Huang Yi Yang Xiao-Jie Lu Jia-Shu Li Shu-Yun Jiang Da-Peng Wang Chun-Xiao Hu Gui-Long Wang Dong-Xiao Huang Guo-Hui Jiao Dong Wei Shu-Gao Ye Jian-An Huang Li Zhou Xiao-Qin Zhang Jian-Xing He | 2020 | Chinese Medical Journal2020，，12： | 19 |
| 13 | Evaluation of the Effects of Standard Rescue Procedure on Severe Trauma Treatment in China显示文摘 | Xiao-Feng Yin Tian-Bing Wang Pei-Xun Zhang Yu-Hui Kou Dian-Ying Zhang Kai Yu De-Cheng Lyu Mao-Zheng Liu Dong-Sheng Zhou Peng Zhang Jue-Hua Jing Wei-Wei Ge Li-Ying Cao Guo-Sheng Wang Shao-Jie Deng Weng-Hua Liu Mao Zhang Yong-An Xu Kun Zhang Bing Li Wei Wang Zhong-Li Gao Cheng-La Yi Bao-Guo Jiang | 2015 | Chinese Medical Journal2015，，10： | 18 |
| 14 | Cost-effectiveness analysis of chemotherapy for advancedgastric cancer in China显示文摘AIM: To assess the economics of various chemotherapeutic regimens for advanced gastric cancer (AGC), and to select the best cost-effective regimen for the common Chinese patients. METHODS: Data source used in this study was the Chinese Biomedical Disk Database. Patients were diagnosed as AGC and any regimen was eligible. Outcome measures included median survival time (MST) and percentage of complete and partial response (CR+PR). Economic statistics was per capita direct medical cost (DMC) of a single cycle. TreeAge Pro Healthcare 2007 software was used to carry out cost-effectiveness and incremental cost-effectiveness analysis. Sensitivity analyses were applied by altering willingness-to-pay and annual discount rate, and also re-analyzed by excluding the studies with apparent heterogeneity. RESULTS: Seven retrospective economics studies on 760 patients were included. 5-fluorouracil-based regimens were universal, and also some new agents were involved, such as docetaxel, paclitaxel, and oxaliplatin. By processing analysis, we could recommend etoposide, leucovorin and 5-fluorouracil (ELF) regimen as preference, with a DMC/MST ratio of 2543 RBM/11.7 mo and a DMC/CR+PR ratio of 2543 RMB/53.3%. Uracil-tegafur, etoposide and cisplatin (FEP) or 5-fluorouracil, adrimycin/epirubin and mitomycin (FAM) regimens could be regarded as optional first-line chemotherapy for AGC in common Chinese patients. With no regard for willingness-to-pay, the docetaxel, cisplatin and 5-fluorouracil (DCF) regimen could be chosen as either a first- or a second-line chemotherapy, with a DMC/CR+PR ratio of 9979 RMB/56.3%. CONCLUSION: 5-fluorouracial regimens are still considered the mainstream for AGC, while new agents such as taxanes are optional. More randomized clinical trials are required before any mandatory recommendation of certain regimens for patients with AGC in China is made. | Xin-Zu Chen Kun Jiang Jian-Kun Hu Bo Zhang Hong-Feng Gou Kun Yang Zhi-Xin Chen Jia-Ping Chen | 2008 | World Journal of Gastroenterology2008，14，17： | 17 |
| 15 | Lysine acetylsalicylate ameliorates lung injury in rats acutely exposed to paraquat显示文摘背景 Paraquat (PQ ) ,有效、广泛地使用的除草剂,当适当地适用消除杂草时,被证明了安全。然而,毒害的 PQ 是极其挫败有高死亡并且与在人的有效处理的缺乏的临床的状况。PQ 主要在肺积累,并且 PQ 毒性的主要分子的机制基于氧化还原作用骑车和细胞内部的氧化压力产生。这研究的目的是评估离氨酸 acetylsalicylate ( LAS )是否能保护肺免受毒害的 PQ 的损坏的伤害并且学习 protection.Methods 的机制毒害的 PQ 的一个模型被 50 mg/kg PQ 的 intragastric 管理在 75 只 Sprague-Dawley 老鼠建立,与 LAS 的 200 mg/kg 由处理列在后面。老鼠随机被划分成假冒, PQ,和 PQ+LAS 组,与 25 在每个组。我们估计了并且比较 malonaldehyde ( MDA )内容, superoxide dismutase 活动(草皮), glutathion peroxidase ( GSH-Px ),和在浆液和肺和 hydroxyproline (忧郁)的过氧化氢酶(猫)内容,病理学的变化,在在天的老鼠的肺的 Bcl-2/Bax 蛋白质的 apoptosis 和表示 1 , 3 , 7 , 14 并且毒害的 21after PQ 和 LAS treatment.Results 比作 PQ 组老鼠,有 LAS 的早处理减少了 MDA 和忧郁内容,并且增加了 SOD , GSH-Px ,并且在浆液的猫活动一而且,肺的病理学的结果表明在 LAS 处理以后,毒害的 PQ 的早表明例如出血,浮肿和煽动性房间的渗入,在某种程度上被改进,并且在肺的 interstitium 的骨胶原纤维也显然是在毒害的 PQ 的这个老鼠模型的 reduced.Conclusion , LAS 有效地改善了肺损害由 PQ 导致了,可能通过 antioxidation ,反纤维变性, anti-apoptosis ,和 anticoagulation 。 | HUANG Wei-dong WANG Jie-zan LU Yuan-qiang DI Ya-min JIANG Jiu- kun ZHANG Qin | 2011 | Chinese Medical Journal2011，，16： | 16 |
| 16 | "Fast-track" and "Minimally Invasive" Surgery for Gastric Cancer显示文摘 | Xin-Xin Liu Hua-Feng Pan Zhi-Wei Jiang Shu Zhang Zhi-Ming Wang Ping Chen Yan Zhao Gang Wang Kun Zhao Jie-Shou Li | 2016 | Chinese Medical Journal2016，，19： | 15 |
| 17 | Development of an indirect competitive ELISA for simultaneous detection of enrofloxacin and ciprofloxacin显示文摘Modified 1-ethyl-3-(3-dimethylaminopropy) carbodiimide(EDC) method was employed to synthesize the artificial antigen of enrofloxacin(ENR),and New Zealand rabbits were used to produce anti-ENR polyclonal antibody(pAb).Based on the checkerboard titration,an indirect competitive enzyme-linked immunosorbent assay(ELISA) standard curve was established.This assay was sensitive and had a linear range from 0.6 to 148.0 μg/kg(R2=0.9567),with the half maximal inhibitory concentration(IC50) and limit of detection(LOD) values of 9.4 μg/kg and 0.2 μg/kg,respectively.Of all the competitive analogues,the produced pAb exhibited a high cross-reactivity to ciprofloxacin(CIP)(87%),the main metabolite of ENR in tissues.After optimization,the matrix effects can be ignored using a 10-fold dilution in beef and 20-fold dilution in pork.The overall recoveries and coefficients of variation(CVs) were in the ranges of 86%-109% and 6.8%-13.1%,respectively.It can be concluded that the established ELISA method is suitable for simultaneous detection of ENR and CIP in animal tissues. | Hai-tang ZHANG Jin-qing JIANG Zi-liang WANG Xin-yao CHANG Xing-you LIU San-hu WANG Kun ZHAO Jin-shan CHEN | 2011 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2011，12，11： | 14 |
| 18 | Magnolol additive as a replacer of antibiotic enhances the growth performance of Linwu ducks显示文摘Magnolol rich in Magnolia officinalis is a bioactive polyphenolic compound. The aim of this study was to examine the effects of magnolol additive(MA) on growth performance, expression levels of antioxidantrelated genes, and intestinal mucosal morphology of Linwu ducks aged from 49 to 70 days, comparing with that of an antibiotic additive(colistin sulfate [CS]). A total of 275,49-day-old ducks were assigned to5 groups with 5 cages of 11 ducks each and fed diets supplemented with 0,100, 200 and 300 mg of MA/kg and 300 mg of CS/kg for 3 weeks, respectively. The results showed that the average daily body weight gain(ADG) was increased significantly in MA-fed groups(200 and 300 mg/kg), compared with the basal diet(BD) group(P < 0.05). The mRNA levels of superoxide dismutase-1(SOD1), manganese superoxide dismutase-2(MnSOD2) and catalase(CAT) were also increased significantly in MA groups(P < 0.05). In addition, hematoxylin and eosin staining revealed that Linwu ducks fed the diets with MA had more intact intestinal mucosa than those fed the BD and CS diets. In addition, ileal villus height, ileal villus height/crypt depth ratio(V/C) and duodenal V/C were also improved significantly(P < 0.05). Taken together, these data demonstrated that MA is an effective feed additive to enhance the growth performance of the Linwu ducks by improving the antioxidant and intestinal mucosal status, suggesting that MA will be a potential additive to replace antibiotic(CS). | Qian Lin Jianfei Zhao Kun Xie Yushi Wang Guili Hu Guitao Jiang Qiuzhong Dai Zhiyong Fan Jianhua He Xi He De-Xing Hou | 2017 | Animal Nutrition2017，，2： | 14 |
| 19 | A Flexible Multi-Layer Map Model Designed for Lane-Level Route Planning in Autonomous Vehicles显示文摘An increasing number of drivers are relying on digital map navigation systems in vehicles or mobile phones to select optimal driving routes in order to save time and improve safety. In the near future, digital map navigation systems are expected to play more important roles in transportation systems. In order to extend current navigation systems to more applications, two fundamental problems must be resolved: the lane-level map model and lane-level route planning. This study proposes solutions to both problems. The current limitation of the lane-level map model is not its accuracy but its flexibility;this study proposes a novel seven-layer map structure, called as Tsinghua map model, which is able to support autonomous driving in a flexible and efficient way. For lane-level route planning, we propose a hierarchical route-searching algorithm to accelerate the planning process, even in the presence of complicated lane networks. In addition, we model the travel costs allocated for lane-level road networks by analyzing vehicle maneuvers in traversing lanes, changing lanes, and turning at intersections. Tests were performed on both a grid network and a real lane-level road network to demonstrate the validity and efficiency of the proposed algorithm. | Kun Jiang Diange Yang Chaoran Liu Tao Zhang Zhongyang Xiao | 2019 | Engineering2019，5，2： | 14 |
| 20 | Transplantation of VEGF165-gene-transfected endothelial progenitor cells in the treatment of chronic venous thrombosis in rats显示文摘背景血栓症的组织和开运河是一个动态、复杂的过程。研究是在我们构造了的长期的静脉的 thrombosis.Methods 上学习干细胞移植和基因 transfection 的 cotherapeutic 效果的这的目的带脉管的 endothelial 生长因素的 recombinant adenoviral 向量 165 (VEGF165 ) 由使用 pAdEasy 系统的基因,它是随后识别了并且放大。同时, endothelial 祖先房间(EPC ) 用 Ficoll 从老鼠骨头髓被孤立,在 EBM-2MV 媒介有教养,并且识别。然后,房间是有 recombinant Ad-VEGF165 的 transfected。EPC 在移植前用 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine (Dil ) 被标记。长期的静脉血栓的一个老鼠模型被劣等的静脉 cava 的部分结扎开发。老鼠随机被划分成 4 个组(n=25,每) :一, Ad-VEGF165/EPC-transplantation 组收到了 1 ml (10 ~ 6 ) Ad-VEGF165/EPCs;B, EPC-transpiantation 组收到了 1 ml (10 ~ 6 ) EPC;C, Ad/EPC-transplantation 组收到了 1 ml (10 ~ 6 ) Ad/EPCs;D,控制组收到了移植媒介的 1 ml。血栓症和邻近的 ceval 墙被收获在移植以后的 28 天;即时量的聚合酶链反应被用来检测脉管的 endothelial 生长的表示水平因素(VEGF ) Mrna;并且西方的弄污被用来在染色的 VEGF 蛋白质 expression.Hematoxylin 曙红测量变化,染色的 immunohistochemical 被执行检测开运河。Neovascularization 被为 von Willebrand 因素(Vwf ) 用抗体染色的 immunohistochemical 检测,它是份量上由在高力量的 microscope.Results 下面数 capillaries 决定了的毛状的密度是的 endothelial cells.The 的一个部件 Ad-VEGF165 被构造,并且 bone-marrow-derived EPC 被栽培并且成功地识别了。我们决定了支持了 EPC 的生长的最佳 transfection 比率。在 transfection 以后, EPC 分泌了 VEGF 蛋白质。在移植以后,在里面进 endothelial 房间的 EPC 和他们的区别的 vivo 幸存被检测与 Dil 污点联系的荧光决定。VEGF Mrna 在组 A 在移植,和 VEGF Mrna 水平以后在组 A, B, C 和 D 被表示比在组 B, C 和 D 的那些显著地高(P < 0.05 ) ;在组 B 和 C 的 VEGF Mrna 层次比在组 D 的那些显著地高(P < 0.05 ) ,并且在在组 B 和 C 的 VEGF Mrna 层次之间没有统计意义。开运河在组 A 的毛状的密度比在组 B 的那些显著地高, C (P < 0.05 ) 并且 D (P < 0.01 ) ;开运河在组 B 和 C 的毛状的密度在组 D 比那显著地高(P < 0.05 ) 。而且,为 EPC 是的组 B 和 C.Conclusions 的价值之间没有统计重要差别成功地由 Ad-VEGF165 的 transfected。合适的 transfection 比率能在移植以后改进 EPC 和 angiogenesis 的提升的可能性的效率。Transfected EPC 引起了静脉血栓症的加速的组织和开运河。 | MENG Qing-you LI Xiao-qiang YU Xiao-bin LEI Feng-rui JIANG Kun LI Chuan-yong | 2010 | Chinese Medical Journal2010，，4： | 14 |
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