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| 1 | Comparison of labeling methods and time course of traumatic brain injury-induced cell death in mice显示文摘BACKGROUND:Various molecular mechanisms of cell death following traumatic brain injury have been previously described.However,the time course of cell death remains unclear.TUNEL and Fluoro-Jade B labeling have been widely used to label apoptotic cells and neuronal degeneration.Propidium iodide (PI) functions as a biomarker of cell death in vivo.OBJECTIVE:To explore the role of PI labeling compared to TUNEL and Fluoro-Jade B staining for detecting neural cell death,and to observe time course of traumatic brain injury-induced cell death in mice.DESIGN,TIME AND SETTING:A randomized,controlled,animal experiment was performed at the Laboratory of Aging and Nervous Diseases,Soochow University from September 2007 to December 2008.MATERIALS:PI (B1221) was purchased from Sigma,USA.TUNEL kit was purchased from Roche Molecular Biochemicals,USA.Fluoro-Jade B was purchased from Chemicon,USA.METHODS:A total of 70 healthy,male,Kunming mice were randomly assigned to sham-surgery (n=5) and model (n=65) groups.Traumatic brain injury was established using the controlled cortical impact method.PI was intraperitoneally injected at 1 hour prior to animal sacrifice.MAIN OUTCOME MEASURES:TUNEL,Fluoro-Jade B,and PI-positive cells were quantified using a double-labeling method to determine the time course of traumatic brain injury-induced cell death.RESULTS:PI labeled cells in an earlier phase of cell death than TUNEL and Fluoro-Jade B labeling.PI-positive cells were observed immediately following injury,and the numbers rapidly increased in injured brain areas at 1 hour,peaked at 24-48 hours,and subsequently decreased at 3-21 days post-injury.TUNEL-labeled cells were significantly increased at 12 hours,while Fluoro-Jade B-labeled cells were increased at 6 hours after injury,with cells still visible at 6-48 hours post-injury.Moreover,a greater number of PI-positive cells were observed compared to TUNEL-and Fluoro-Jade B-labeled cells.CONCLUSION:PI labeling is more sensitive and reliable than TUNEL and Fluoro-Jade B staining for detecting cell death following traumatic brain injury.Moreover,PI labeling can function as a reliable marker to estimate the entire time course of cell death. | Chengliang Luo Xiping Chen Hong Ni Qianqian Li Rui Yang Yuxia Sun Guangyou Zhu Luyang Tao | 2010 | Neural Regeneration Research2010，5，9： | 6 |
| 2 | Assessment of cognitive dysfunction in traumatic brain injury patients:a review显示文摘Traumatic brain injury(TBI)is one of the major causes of human mortality and morbidity in the world.Brain injury could affect the core of a person’s being–their thinking,memory,personality and behaviour.Electrophysiological markers from the human electroencephalogram and brain imaging provide a rich source of data which helps to elucidate specific processing impairments in TBI patients.To assess the cognitive and social function in traumatic brain injury patients,this review will focus on some of methods for assessing the disabling cognitive and social function deficits induced by TBI.There are many new technologies available to address TBI and recognition related questions.Integration of the various techniques will help to facilitate our comprehending of TBI,cognitive function and social function,and improve treatment and rehabilitation. | Huiyan Sun Chengliang Luo Xiping Chen Luyang Tao | 2017 | Forensic Sciences Research2017，2，4： | 5 |
| 3 | A correlation study of the expression of resistin and glycometabolism in muscle tissue after traumatic brain injury in rats显示文摘 | Jin Peng Zhu Lielie Zhang Jiasheng Xie Songling Pan Da Wen Hao Meng Weiyang Lin Luyang Chen Daqing | 2014 | Chinese Journal of Traumatology2014，17，3： | 5 |
| 4 | Highly dispersed Co-Mo sulfide nanoparticles on reduced graphene oxide for lithium and sodium ion storage显示文摘A novel hybrid,highly dispersed spinel Co-Mo sulfide nanoparticles on reduced graphene oxide(Co3S4/CoMo2S4@rGO),is reported as anode for lithium and sodium ion storage.The hybrid is synthesized by one-step hydrothermal method but exhibits excellent lithium and sodium storage performances.The as-synthesized Co3S4/CoMo2S4@rGO presents reversible capacity of 595.4 mA·h·g^−1 and 408.8 mA·h·g^−1 after 100 cycles at a current density of 0.2 A·g^−1 for lithium and sodium ion storages,respectively.Such superior performances are attributed to the unique composition and structure of Co3S4/CoMo2S4@rGO.The rGO provides a good electronically conductive network and ensures the formation of spinel Co3S4/CoMo2S4 nanoparticles,the Co3S4/CoMo2S4 nanoparticles provide large reaction surface for lithium and sodium intercalation/deintercalation,and the spinel structure allows fast lithium and sodium ion diffusion in three dimensions. | Yuqing Liao Chun Wu Yaotang Zhong Min Chen Luyang Cai Huirong Wang Xiang Liu Guozhong Cao Weishan Li | 2020 | Nano Research2020，13，1： | 4 |
| 5 | Design and measurement of signal processing system for cavity beam position monitor显示文摘In this paper,in order to achieve the output signal processing of cavity beam position monitor(CBPM),we develop a digital intermediate frequency receiver architecture based signal processing system,which consists of radio frequency(RF) front end and high speed data acquisition board.The beam position resolution in the CBPM signal processing system is superior to 1μm.Two signal processing algorithms,fast Fourier transform(FFT) and digital down converter(DDC),are evaluated offline using MATLAB platform,and both can be used to achieve,the CW input signal,position resolutions of 0.31μm and 0.10μm at -16 dBm.The DDC algorithm for its good compatibility is downloaded into the FPGA to realize online measurement,reaching the position resolution of 0.49μm due to truncation error.The whole system works well and the performance meets design target. | WANG Baopeng LENG Yongbin YU Luyang ZHOU Weimin YUAN Renxian CHEN Zhichu | 2013 | Nuclear Science and Techniques2013，24，2： | 3 |
| 6 | Surface disorder engineering in ZnCdS for cocatalyst free visible light driven hydrogen production显示文摘Metal chalcogenide solid solution,especially ZnCdS,has been intensively investigated in photocatalytic H_(2) generation due to their cost-effective synthetic procedure and adjustable band structures.In this work,we report on the defect engineering of ZnCdS with surface disorder layer by simple room temperature Li-ethylenediamine(Li-EDA)treatment.Experimental results confirm the formation of unusual Zn and S dual vacancies,where rich S vacancies(Vs)served as electron trapping sites,meanwhile Zn vacancies(Vzn)served as hole trapping sites.The refined structure significantly facilitates the photo charge carrier transfer and improves photocatalytic properties of ZnCdS.The disordered ZnCdS shows a highest photocatalytic H_(2) production rate of 33.6 mmol·g^(-1)·h^(-1) under visible light with superior photocatalytic stabilities,which is 7.3 times higher than pristine ZnCdS and 7 times of Pt(1 wt.%)loaded ZnCdS. | Enna Ha Shuhong Ruan Danyang Li Yuanmin Zhu Yanping Chen Jiangyuan Qiu Zhaohui Chen Tingting Xu Jingyun Su Luyang Wang Junqing Hu | 2022 | Nano Research2022，15，2： | 3 |
| 7 | The Effect of Super Volcanic Eruptions on Ozone Depletion in a Chemistry–Climate Model显示文摘With the gradual yet unequivocal phasing out of ozone depleting substances(ODSs), the environmental crisis caused by the discovery of an ozone hole over the Antarctic has lessened in severity and a promising recovery of the ozone layer is predicted in this century. However, strong volcanic activity can also cause ozone depletion that might be severe enough to threaten the existence of life on Earth. In this study, a transport model and a coupled chemistry–climate model were used to simulate the impacts of super volcanoes on ozone depletion. The volcanic eruptions in the experiments were the 1991 Mount Pinatubo eruption and a 100 × Pinatubo size eruption. The results show that the percentage of global mean total column ozone depletion in the 2050 RCP8.5 100 × Pinatubo scenario is approximately 6% compared to two years before the eruption and 6.4% in tropics. An identical simulation, 100 × Pinatubo eruption only with natural source ODSs, produces an ozone depletion of 2.5% compared to two years before the eruption, and with 4.4% loss in the tropics. Based on the model results,the reduced ODSs and stratospheric cooling lighten the ozone depletion after super volcanic eruption. | Luyang XU Ke WEI Xue WU S. P. SMYSHLYAEV Wen CHEN V. Ya. GALIN | 2019 | Advances in Atmospheric Sciences2019，36，8： | 3 |
| 8 | 3D printing of hydroxyapatite/tricalcium phosphate scaffold with hierarchical porous structure for bone regeneration显示文摘Three-dimensional(3D)-printed scaffolds have attracted considerable attention in recent years as they provide a suitable environment for bone cell tissue regeneration and can be customized in shape.Among many other challenges,the material composition and geometric structure have major impacts on the performance of scaffolds.Hydroxyapatite and tricalcium phosphate(HA/TCP),as the major constituents of natural bone and teeth,possess attractive biological properties and are widely used in bone scaffold fabrication.Many fabrication methods have been investigated in attempts to achieve HA/TCP scaffolds with microporous structure enabling cell growth and nutrient transport.However,current 3D printing methods can only achieve the fabrication of HA/TCP scaffolds with certain range of microporous structure.To overcome this challenge,we developed a slurry-based microscale mask image projection stereolithography,allowing us to form a HA/TCP-based photocurable suspension with complex geometry including biomimetic features and hierarchical porosity.Here,the curing performance and physical properties of the HA/TCP suspension were investigated,and a circular movement process for the fabrication of highly viscous HA/TCP suspension was developed.Based on these investigations,the scaffold composition was optimized.We determined that a 30 wt%HA/TCP scaffold with biomimetic hierarchical structure exhibited superior mechanical properties and porosity.Cell proliferation was investigated in vitro,and the surgery was conducted in a nude mouse in vivo model of long bone with cranial neural crest cells and bone marrow mesenchymal stem cells.The results showed our 3D-printed HA/TCP scaffold with biomimetic hierarchical structure is biocompatible and has sufficient mechanical strength for surgery. | Xiangjia Li Yuan Yuan Luyang Liu Yuen‑Shan Leung Yiyu Chen Yuxing Guo Yang Chai Yong Chen | 2020 | Bio-Design and Manufacturing2020，3，1： | 3 |
| 9 | Screening of Anti-tumor Effective Extracts from Sedum bulbiferum Makino and HPLC Determination of Quercetin and Kaempferol in This Herbal Medicine显示文摘[Objectives] To investigate the anti-tumor activity of Sedum bulbiferum Makino in vitro,and establish a HPLC method for determination of quercetin and kaempferol in S. bulbiferum. [Methods] The inhibitory activities of different extracts and total flavonoids of S. bulbiferum on proliferation of three kinds of cancer cells( Hep G2,EC109,SW480) were tested by MTT assay. HPLC method for determination of quercetin and kaempferol in S. bulbiferum was established. [Results]The growth and proliferation of the three kinds of cancer cells were all significantly inhibited by ethyl acetate fraction and total flavonoids isolated from S. bulbiferum. With each extraction concentration increasing,stronger anti-tumor activity was found. The linear ranges of quercetin and kaempferol were 0. 03-0. 36 μg( R = 0. 999 9) and0. 08-0. 96 μg( R = 0. 999 9),and the average recoveries were 98. 90%( RSD = 1. 15%) and 98. 27%( RSD = 1. 70%),respectively.[Conclusions]S. bulbiferum has significant anti-tumor activity in vitro. The HPLC method established was accurate,reproducible,and could be used for quality control of this crude drug. | Liming MENG Xiangluan WAN Luyang LI Jing HU Pu CHEN Dingrong WAN | 2019 | Medicinal Plant2019，10，3： | 2 |
| 10 | Monitoring the charge bunch-by-bunch for the SSRF storage ring:Development and application显示文摘Bunch charge uniformity controlling is very important for top-up operation of the storage ring.In order to monitor filling pattern and measure the bunch charge precisely,a PXI waveform digitizer-based data acquisition system has been developed to retrieve bunch charge information from BPM pickup signals.An effective sampling rate is extended to 400 GHz by waveform rebuilding technology,which overlays multi-turn data into single turn with real time sampling rate of 8 GHz.An on-line evaluation indicates that resolution and linearity of the charge measurement are better than 0.5% at input range of 0.5-12nC. | LENG Yongbin YAN Yingbin YU Luyang YUAN Renxian CHEN Zhichu ZHOU Weimin | 2010 | Nuclear Science and Techniques2010，21，4： | 2 |
| 11 | Non-Structural Protein 5 of Zika Virus Interacts with p53 in Human Neural Progenitor Cells and Induces p53-Mediated Apoptosis显示文摘Zika virus(ZIKV) infection could disrupt neurogenesis and cause microcephaly in neonates by targeting neural progenitor cells(NPCs). The tumor suppressor p53-mediated cell cycle arrest and apoptotic cell death have been suggested to be activated upon ZIKV infection, yet the detailed mechanism is not well understood. In the present study, we investigated the effects of ZIKV-encoded proteins in the activation of p53 signaling pathway and found that, among the ten viral proteins,the nonstructural protein 5(NS5) of ZIKV most significantly activated the transcription of p53 target genes. Using the immunoprecipitation-coupled mass spectrometry approach, we identified that ZIKV-NS5 interacted with p53 protein. The NS5-p53 interaction was further confirmed by co-immunoprecipitation and GST pull-down assays. In addition, the MTase domain of NS5 and the C-terminal domain of p53 were mapped to be responsible for the interaction between these two proteins. We further showed that ZIKV-NS5 was colocalized with p53 and increased its protein level in the nuclei and able to prolong the half-life of p53. Furthermore, lentivirus-mediated expression of ZIKV-NS5 in hNPCs led to an apparent cell death phenotype. ZIKV-NS5 promoted the cleavage of PARP1 and significantly increased the cell apoptosis of h NPCs.Taken together, these findings revealed that ZIKV-NS5 is a previously undiscovered regulator of p53-mediated apoptosis in hNPCs, which may contribute to the ZIKV-caused abnormal neurodevelopment. | Ping Li Hualian Jiang Hong Peng Weijie Zeng Yongheng Zhong Miao He Luyang Xie Junhai Chen Deyin Guo Junyu Wu Chun-Mei Li | 2021 | Virologica Sinica2021，36，6： | 2 |
| 12 | A natural variation of an SVP MADS-box transcription factor in Triticum petropavlovskyi leads to its ectopic expression and contributes to elongated glume显示文摘Dear Editor,Triticum petropavlovskyi,commonly known as Xinjiang rice wheat,is a unique hexaploidy wheat grown in the Xinjiang area.T.petropavlovskyi is marked by its elongated glume(Eg)trait,which is controlled by gene P1pet located on chromosome 7A(Wang et al.,2002;Watanabe and Imamura,2002).The origin of T.petropavlovskyi has been disputed.It is generally accepted that P1pet was introduced from tetraploid polish wheat(T.polonicum)via a hybridization with hexaploid common wheat(T.aestivum)(Akond et al.,2008;Chen et al.,2016). | Jin Xiao Yan Chen Yifan Lu Zhipeng Liu Dongmei Si Tao Xu Li Sun Zongkuan Wang Chunxia Yuan Haojie Sun Xu Zhang Mingxing Wen Luyang Wei Wenli Zhang Haiyan Wang Xiue Wang | 2021 | Molecular Plant2021，14，9： | 2 |
| 13 | A mild solvothermal route to nanocrystalline titanium diboride显示文摘 | Yunle Gu Yitai Qian Luyang Chen Fu Zhou | 2002 | Journal of Alloys and Compounds2002，，1： | 1 |
| 14 | A room-temperature synthesis of nanocrystalline vanadium nitride显示文摘 | Chen Luyang Gu Yunle Shi Liang | 2004 | Solid State Communications2004，132，5： | 1 |
| 15 | A simple protocol for bulk synthesis of TiC hollow spheres from carbon nanotubes显示文摘 | Gu Yunle Chen Luyang Li Zhefeng | 2004 | Carbon2004，42，1： | 1 |
| 16 | Solvothermal preparation of amorphous carbon nanotubes and Fe/C coaxial nanocables from sulfur, ferroeene, and benzene 显示文摘 | Luo Tao Chen Luyang Bao Keyan | 2006 | Carbon2006，44，13： | 1 |
| 17 | Far-infraredassisted extraction followed by MEKC for the simultaneous determination of flavones and phenolic acids in the leaves of Rhododendron mucronulatum Turcz显示文摘 | FU Yuejiao ZHANG Luyang CHEN Gang | 2012 | Journal of separation science2012，35，3： | 1 |
| 18 | 显示文摘 | Zhao Aiwu Luo Tao Chen Luyang | 2006 | Materials Chemistry and Physics2006，99，： | 1 |
| 19 | Stratosphere amplifies the global climate effect of wildfires显示文摘The summer of 2019 witnessed a great number of wildfires around the globe.For example,wildfires scorched huge swaths of Alaska,British Columbia in Canada,and parts of Greenland.And forest fires in Siberia and the Far East of Russia were also very serious,affecting nearly 3 million hectares of land,and putting some parts of Russia into a state of emergency.In particular,forest fires in the Amazon region had attracted global attention.According to Science,as of August 24,BraziPs National Institute for Space Research had counted more than 41000 fires this year,86%more than last year,compared with 22000 in the same period last year.Scientists in Brazil and elsewhere believe that the recent increase of wildfires is closely related to the increasing ac・tivities of deforestation(Arruda et al.,2019;Escobar,2019). | Ke WEI Wen CHEN Luyang XU Chunjiang ZHOU | 2020 | Science China Earth Sciences2020，63，2： | 1 |
| 20 | A low temperature synthesis of crystalline B 4 C ultrafine powders显示文摘 | Liang Shi Yunle Gu Luyang Chen Yitai Qian Zeheng Yang Jianhua Ma | 2003 | Solid State Communications2003，，1： | 1 |
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