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| 1 | Degradation Characteristics and Community Structure of a Hydrocarbon Degrading Bacterial Consortium显示文摘A hydrocarbon degrading bacterial consortium KO5-2 was isolated from oil-contaminated soil of Karamay in Xinjiang, China, which could remove 56.9% of 10 g/L total petroleum hydrocarbons(TPH) at 30 ℃ after 7 days of incubation, and could also remove 100% of fluorene, 98.93% of phenanthrene and 65.73% of pyrene within 3, 7 and 9 days, respectively. Twelve strains from six different genera were isolated from KO5-2 and only eight ones were able to utilize the TPH. The denaturing gradient gel electrophoresis(DGGE) was used to investigate the microbial community shifts in five different carbon sources(including TPH, saturated hydrocarbons, fluorene, phenanthrene and pyrene). The test results indicated that the community compositions of KO5-2 in carbon sources of TPH and saturated hydrocarbons, respectively, were roughly the same, while they were distinctive in the three different carbon sources of PAHs. Rhodococcus sp. and Pseudomonas sp. could survive in the five kinds of carbon sources. Bacillus sp., Sphingomonas sp. and Ochrobactrum sp. likely played key roles in the degradation of saturated hydrocarbons, PAHs and phenanthrene, respectively. This study showed that specific bacterial phylotypes were associated with different contaminants and complex interactions between bacterial species, and the medium conditions influenced the biodegradation capacity of the microbial communities involved in bioremediation processes. | Li Zheng Gu Guizhou Zhao Chaocheng Zhao Dongfeng | 2015 | China Petroleum Processing & Petrochemical Technology2015，17，3： | 7 |
| 2 | Optimization for Microbial Degradation of Dibenzothiophene by Pseudomonas sp. LKY-5 Using Response Surface Methodology显示文摘In this research, the degradation of dibenzothiophene(DBT) was investigated by using Pseudomonas sp. LKY-5 isolated from oil contaminated soil. The response surface methodology(RSM) based on the Box-Behnken design(BBD) was applied for evaluating the interactive effects of four independent variables including substrate concentration, temperature, pH and agitation rate on the DBT removal response. A total of 29 experiments for four factors at three levels were conducted in present study. A second-order regression model was then developed, and the analysis of variance(ANOVA) illustrated that the proposed quadratic model could be utilized to navigate the design space. The value of determination coefficient(R2=0.953 4) indicated a satisfactory agreement between the quadratic model and the experimental data. It was found that DBT removal was more significantly affected(P<0.000 1) by substrate concentration compared with other three parameters. An 100% degradation of DBT could be obtained by Pseudomonas sp. LKY-5 at a substrate concentration of 100 mg/L. | Li Lin Zhao Chaocheng Liu Qiyou Zhang Yunbo Liu Chunshuang Xue Jianliang | 2014 | China Petroleum Processing & Petrochemical Technology2014，16，1： | 5 |
| 3 | Corrosion Inhibition by Co-Immobilized Lysozyme and Lipase in Circulating Cooling Water System显示文摘The corrosion inhibition performance of co-immobilized lysozyme and lipase was investigated in a recirculating cooling water system. Four methods were carried out in co-immobilization, and the operating parameters were optimized by using the respond surface methodology(RSM). The corrosion inhibition performance of co-immobilized lipase and lysozyme was evaluated by weight loss measurements and electrochemical measurements. The results revealed that the optimal co-immobilization method should be the sequential immobilization of lysozyme and then lipase. The inhibition efficiency was 86.10% under the optimal co-immobilized conditions. Electrochemical data showed that co-immobilized lysozyme and lipase was a mixed-type inhibitor and the corrosion inhibition efficiency was 81%. | Liu Fang Lü Yucui Jiang Guofei Chen Xiaorui Sun Juan Zhao Chaocheng | 2017 | China Petroleum Processing & Petrochemical Technology2017，19，2： | 3 |
| 4 | Comparison and Analysis of Toluene Adsorption Properties of ZSM-5 Molecular Sieve Treated by Different Modification Methods: Adsorption Kinetic and Mechanism Studies显示文摘MFI molecular sieve with high specific surface area,adjustable pore size and low production cost have been recognized as an effective adsorbent for VOCs removal.In this paper,NaOH solution was used to etch ZSM-5 to increase specific surface area and micro-mesoporous content.Graphene oxide(GO)was loaded on ZSM-5 by ultrasonic-assisted heating immersion method,and largeπ-bond structure and oxygen-containing functional groups were added to ZSM-5 for improving the composite adsorption performance.In addition,the properties of OH-ZSM-5 and ZSM-5@GO composites for toluene adsorption under different factors were also studied.It was positively correlated with initial concentration and adsorbent mass,but was negatively correlated with temperature.Meanwhile,the results showed that the saturated adsorption capacity of OH-ZSM-5(107.3 mg/g)was 1.34 times higher than that of ZG-15%(80.2 mg/g).The pseudo-first-order and pseudo-second-order kinetic model can well describe the adsorption behavior of toluene on the OH-ZSM-5 and ZG-15%,respectively.The adsorption mechanism of OH-ZSM-5 was mainly pore-filled adsorption.However,the adsorption mechanisms of ZSM-5@GO composite were pore-filled adsorption,π-πinteraction,and H-bond interaction.This study will help to design a new strategy for enhancing the performance of traditional adsorbent ZSM-5 in VOCs removal. | Zhang Shuai Li Meng Li Wei Li Yuwei Liu Fang Xue Ming Wang Yongqiang Zhao Chaocheng | 2021 | China Petroleum Processing & Petrochemical Technology2021，23，1： | 2 |
| 5 | Laccase Immobilized on Mesoporous Silica Materials and Its Corrosion Inhibition Performance in Circulating Cooling Water System显示文摘Mesoporous SiO_2 microspheres were synthesized using the sol-gel method and were characterized by TEM, FT-IR and BET techniques. The diameter of the microspheres is about 100—150 nm, and the average mesopore diameter is 2.55 nm, while the specific surface area is 1 088.9 m2/g. Mesoporous SiO_2 microspheres adsorb glutaraldehyde and immobilize laccase by means of the aldehyde group in glutaral which can react with the amidogen of laccase. The immobilization conditions were optimized at a glutaraldehyde concentration of 0.75%, a crosslinking time of 8 h, a laccase concentration of 0.04 L/L and an immobilization time of 10 h. When diesel leakage concentration was 80 mg/L, the highest corrosion inhibition efficiency of immobilized laccase reached 49.23%, which was slightly lower than the corrosion inhibition efficiency of free laccase(59%). The diesel degradation ratio could reach up to 45%. It has been proved that the immobilized laccase could degrade diesel to inhibit corrosion. | Liu Fang Lü Yucui Zhong Huiyun Zhang Shuang Fan Fengtao Zhao Chaocheng | 2015 | China Petroleum Processing & Petrochemical Technology2015，17，4： | 2 |
| 6 | Simultaneous Biological Removal of p-Cresol, Sulfide and Nitrate by Denitrification显示文摘Biological removal of sulfide, nitrate, and p-cresol at a loading rate of 340 gS/(m^3 · d), 340 gN/(m^3 · d) and600 gC/(m^3 · d), respectively, was achieved in an upflow anaerobic sludge blanket(UASB) reactor. The ratio of C/S could affect the elemental sulfur(S^0) accumulation, and the C/S ratio of 1.75:1 was optimal for S^0 accumulation. Strains Pseudomonas sp., Simplicispira sp., and Rhizobium sp. could likely yield heterotrophs. Strains Arcobacter sp.,Sulfurimonas sp., Sulfurovum sp., and Sulfurospirillum sp. are the autotrophics required for the proposed UASB system. At high loading rate, the autotrophic denitrification pathway declined faster than the heterotrophic pathway. | Liu Chunshuang Li Wenfei Zhao Dongfeng Li Wei Li Xuechen Zhao Chaocheng Liu Fang | 2017 | China Petroleum Processing & Petrochemical Technology2017，19，4： | 2 |
| 7 | Isolation and Characterization of a Thermophilic Oil-Degrading Bacterial Consortium显示文摘In this study, a thermophilic oil-degrading bacterial consortium KO8-2 growing within the temperature range of 45-65 ℃ (with 55 ℃ being the optimum temperature) was isolated from oil-contaminated soil of Karamay in Xinjiang, China. Denaturing gradient gel electrophoresis (DGGE) showed that there were nine strains included in KO8-2, which originated from the genera of Bacillus, Geobacillus and Clostridium. They all belonged to thermophilic bacteria, and had been previously proved as degraders of at least one petroleum fraction. The crude oil degraded by KO8-2 was analyzed by infrared spectrophotometry, hydrocarbon group type analysis and gas chromatography. The results indicated that the bacterial consortium KO8-2 was able to utilize 64.33% of saturates, 27.06% of aromatics, 13.24% of resins and the oil removal efficiency reached up to 58.73% at 55 ℃ when the oil concentration was 10 g/L. Detailed analysis showed that KO8-2 was able to utilize the hydrocarbon components before C 19 , and the n-alkanes ranging from C 20 -C 33 were signifi- cantly degraded. The ratios of nC 17 /Pr and nC 18 /Ph were 3.12 and 3.87, respectively, before degradation, whereas after degradation the ratios reduced to 0.21 and 0.38, respectively. Compared with the control sample, the oil removal efficiency in KO8-2 composting reactor reached 50.12% after a degradation duration of 60 days. | Gu Guizhou Li Zheng Zhao Dongfeng Zhao Chaocheng | 2013 | China Petroleum Processing & Petrochemical Technology2013，15，2： | 2 |
| 8 | Rapid Acclimation of Methanogenic Granular Sludge into Autotrophic Partial Denitrification Granules显示文摘Rapid formation of autotrophic partial denitrification(APD)granules is of practical interest to start up an expanded granular sludge bed reactor for wastewater treatment.This study demonstrates that methanogenic granules can be easily acclimated into autotrophic partial denitrification granules in one day,with the ability to remove 82%of 2.7 kg-S/(m^3·d)sulfide into S^0 and to convert 97%of 0.9 kg-N/(m^3·d)nitrate into nitrite,which can provide a promising feedstock for anaerobic ammonia oxidation process.Arcobacter sp.is essential for S^0 accumulation.Under high loadings,the abundance of Arcobacter sp.decreased,while on the contrary the abundance of unclassified_p_Firmicutes increased,leading to the deterioration of autotrophic partial denitrification performance.The granules performance could be recovered by adopting the strategies of properly reducing the influent loadings. | Liu Chunshuang Li Xuechen Zhang Xiaofei Bai Xue Guo Yadong Wang Yongxing Zhao Chaocheng | 2019 | China Petroleum Processing & Petrochemical Technology2019，21，3： | 2 |
| 9 | Optimization of reed-specific degrading bacteria by response surfaces for remediation of crude oil-polluted soil in Xinjiang,China显示文摘This paper discussed the optimization of conditions for remediation of crude oil-polluted soil based on pot experiment by applying reed-specific degrading bacteria,and using response surfaces methodology.We took the initial crude oil concentration,the amount of inoculation,the ratio of nitrogen and phosphorus,and the use of surfactant(Tween-80)as independent variables(factors),and the degrading ratio of crude oil as the dependent variable(response)after a 90-day experiment.The experiment explored the impacts of each independent variable and their interactions on the bioremediation of crude oil-polluted soil using the Box-Behnken design.Working with a simulated forecasting model the study obtained optimization values for the treatment parameters of 200 g/kg of the reed+specific degrading bacteria,a nitrogen to phosphorus ratio of about 6.0,and 0.2% surfactant.Under experimental conditions,for crude oil concentrations of 10,30 and 50 g/kg,the optimal effects of the treatments achieved 71.87%,66.61% and 54.52% degradation of the crude oil,respectively.The results can provide a basis for the technical development of plant-microorganism combined bioremediation of crude oil-polluted soil. | YaMing DONG ChaoCheng ZHAO Yun CAI DongFeng ZHAO | 2013 | Journal of Arid Land2013，5，3： | 2 |
| 10 | Effects of Nutrients on Biofouling Formation and Preponderant Bacteria Diversity in Recirculating Cooling Water System显示文摘一系列直角的数组实验作为主要用碳来源,氨氮和全部的磷(TP ) 被进行影响因素在使水系统凉下来的 recirculating 在 biofouling 形成和优势的细菌差异上调查营养素的效果。碳来源被表明是影响 biofouling 形成的最重要的决定因素。当 BOD5, NH4+-N 和 TP 分别地是 25, 10,和 1 mg/L 时,最小的 biofouling 结果被获得。然后,在 biofouling 的优势的细菌紧张集中在 twotypical 文化条件下面(否定、有利)被识别与种系发生的联营分析使用传统的生物化学的方法和进一步分子的生物学技术,它显示了那 Enterobacteriaceae Enterobacter , Micrococcaceae 葡萄球菌, Bacillaceae 杆菌, Enterobacteriaceae 盲螈属, Neisseriaceae Neis-seria 和 Pseudomonadaceae Pseudomonas 在否定状况下面是主导的,当时 | Ma Tao Liu Fang Zhang Guizhi Zhao Chaocheng (College of Chemistry and Chemical Engineering,China University of Petroleum,Qingdao 266555) | 2010 | China Petroleum Processing & Petrochemical Technology2010，12，4： | 2 |
| 11 | Study on Isolation, Identification of A Petroleum Hydrocarbon Degrading Bacterium Bacillus fusiformis sp. and Influence of Environmental Factors on Degradation Efficiency显示文摘A hydrocarbon degrading bacterium KL2-13 was isolated from ten sites of oil contaminated soil in the Karamay oilfield. It was identified as the Bacillus fusiformis sp. bacterium based on its morphological and physiological characteristics and the 16S rDNA sequence analysis. The factors influencing the hydrocarbon degradation by the bacterium KL2-13 were determined. The test results have showed that the hydrocarbon degrading bacterium KL2-13 requires an optimum pH range of 6-8, and the optimum inoculation quantity is 3%. The low-concentration metal ions Fe2+, Mg2+ and Ca2+ can improve the degradation ability of the bacteria KL2-13.Atoo low concentration of Tween-80 does not show obvious promotion to the de- grading bacterium KL2-13, and an excessively high concentration can decrease the degradation ability of the bacterium, the best dosage of which is 2%. The hydrocarbon degrading rate reached 59.07%±0.37% under the optimum culture conditions. | Zhao Dongfeng Wu Weilin Zhang Yunbo Liu Qiyou Yang Haibin Zhao Chaocheng | 2011 | China Petroleum Processing & Petrochemical Technology2011，13，4： | 1 |
| 12 | Enhanced Performance of Denitrifying Sulfide Removal Process by 1,2-Naphthoquinone-4-Sulphonate显示文摘The denitrifying sulfide removal(DSR) process with bio-granules comprising both heterotrophic and autotrophic denitrifiers can simultaneously convert nitrate, sulfide and acetate species into di-nitrogen gas, elemental sulfur and carbon dioxide, respectively, at high loading rates. This study has determined that the reaction rate of sulfide oxidized into sulfur could be enhanced in the presence of 1,2-naphthoquinone-4-sulphonate(NQS). The presence of NQS mitigated the inhibition effects of sulfide species on denitrification. Furthermore, the reaction rates of nitrate and acetate to nitrogen gas and CO_2, respectively, were also promoted in the presence of NQS, thereby enhancing the performance of DSR granules. The advantages and disadvantages of applying the NQS-DSR process are discussed. | Liu Chunshuang Han Kang Zhao Dongfeng Guo Yadonag Liu Lihong Liu Fang Zhao Chaocheng | 2016 | China Petroleum Processing & Petrochemical Technology2016，18，1： | 1 |
| 13 | Acidification and bubble template derived porous g-C_(3)N_(4) for efficient photodegradation and hydrogen evolution显示文摘Graphitic carbon nitride(g-C_(3)N_(4))as a metal-free candidate of photocatalyst has received worldwide attention because of its great potentials in solar light-induced degradation and hydrogen evolution,yet the industrial application is seriously hindered by the small specific surface area and rapid recombination rate of carriers.Herein,we demonstrate that porous g-C_(3)N_(4)(HCl-CNU-X)can be prepared via the copolymerization of acidified melamine and a green bubble template(urea).Transmission electron microscopy and nitrogen sorption characterization results show that the prepared HCl-CNU-X possesses an in-plane porous structure and large specific surface area,enabling the exposure of more accessible active sites.As a result,HCl-CNU-X exhibits both enhanced photocatalytic tetracycline hydrochloride degradation and higher hydrogen evolution than bulk g-C_(3)N_(4).The boosted photocatalytic performance was ascribed to the formation of the porous structure,which dramatically promotes the separation of charge-carriers and facilitates the electron transfer.This work demonstrates that the acidification of nitrogen-rich precursors combined with a bubble-template can develop a new paradigm of highly porous photocatalysts for environmental remediation and water splitting. | Yixuan Wang Fengting He Lin Chen Jie Shang Jiajia Wang Shuaijun Wang Huimin Song Jinqiang Zhang Chaocheng Zhao Shaobin Wang Hongqi Sun | 2020 | Chinese Chemical Letters2020，31，10： | 1 |
| 14 | Performance and Modeling of an Up-flow Anaerobic Sludge Blanket(UASB) Reactor for Treating High Salinity Wastewater from Heavy Oil Production显示文摘In this study,an up-flow anaerobic sludge blanket(UASB) reactor was applied to treat the high salinity wastewater from heavy oil production process.At a HRT of ≥24 h,the COD removal reached as high as 65.08% at an influent COD ranging from 350mg/L to 640mg/L.An average of 74.33% oil reduction was also achieved in the UASB reactor at an initial oil concentration between 112mg/L and 205mg/L.These results indicated that this heavy oil production related wastewater could be degraded efficiently in the UASB reactor.Granular sludge was formed in this reactor.In addition,two models,built on the back propagation neural network(BPNN) theory and linear regression techniques were developed for the simulation of the UASB system performance in the oily wastewater biodegradation.The average error of COD and oil removal was-0.65% and 0.84%,respectively.The results indicated that the models built on the BPNN theory were wellfitted to the detected data,and were able to simulate and predict the removal of COD and oil by the UASB reactor. | Liu Chunshuang Zhao Dongfeng Guo Yadong Zhao Chaocheng | 2012 | China Petroleum Processing & Petrochemical Technology2012，14，3： | 1 |
| 15 | Tertiary treatment of textile wastewater with combined media biological ael'ated filter (CMBAF) at different hydraulic loadings and dissolved oxygen concentrations显示文摘 | Liu Fang Zhao Chaocheng Zhao Dongfeng | 2008 | J Hazard Mater2008，60，1： | 1 |
| 16 | Combined Use of Co-immobilized Lysozyme and Lipase and Chemical Inhibitors in Circulating Cooling Water System显示文摘In order to improve the stability and corrosion inhibition performance of bioenzyme, lipase and lsozyme were co-immobilized on the mesoporous molecular sieve MCM-41 by the adsorption method. Then the immobilized enzymes were combined with amino trimethylene phosphonic acid and polyaspartic acid to prevent corrosion caused by circulating cooling water. The weight-loss method and electrochemical techniques were used to evaluate the performance of composite inhibitors. The co-immobilized lysozyme and lipase achieved good inhibition effects. After they were combined with amino trimethylene phosphonic acid and polyaspartic acid, the corrosion inhibition properties were further improved. The inhibition efficiency was promoted to 94.4%. During the corrosion inhibition process, the immobilized enzymes played an important role. The addition of corrosion inhibitor could inhibit the anodic dissolution and cathodic hydrogen evolution process of carbon steel at the same time. The adsorption of co-immobilized lysozyme and lipase composite inhibitor on the steel surface was a joint action involving physical adsorption and chemical adsorption. | Jiang Guofei Chen Xiaorui Lu Xin Liu Fang Wang Yongqiang Zhao Chaocheng Sun Juan | 2018 | China Petroleum Processing & Petrochemical Technology2018，20，4： | 1 |
| 17 | Enhanced activity of ZnS(111) by N/Cu co-doping:Accelerated degradation of organic pollutants under visible light显示文摘High-efficiency photocatalysts are of great significance for the application of photocatalytic technology in water treatment.In this study,N/Cu co-doped ZnS nanosphere photocatalys(N/Cu-ZnS) is synthesized by a hydrothermal method for the first time.After doping,the tex ture of nanosphere becomes loose,the nanometer diameter is reduced,making the specific surface area of catalyst increased from 34.73 to 101.59 m^(2)/g.The characterization results show that more ZnS (111) crystal planes are exposed by N/Cu co-doping;the calculations of density functional theory show that N/Cu co-doping can increase the catalytic activity of the ZnS (111) crystal plane,enhance the adsorption capacity of (111) crystal plane to O_(2)and promote the generation of·O_(2)-.The energy levels of the introduced impurities can be hybridized with the energy levels of S and Zn at the top of valence band and the bottom o conduction band,which makes the band gap narrower,thus enhancing the absorption o visible light.Compared with pure ZnS,the degradation rates of 2,4-dichlorophenol (2,4-DCP and tetracycline (TC) by N/Cu-ZnS under visible light (>420 nm) are increased by 83.7 and51 times,respectively.In this research,a promising photocatalyst for photocatalytic degra dation of organic pollutants in wastewater is provided. | Guofei Jiang Benjie Zhu Junzhi Sun Fang Liu Yongqiang Wang Chaocheng Zhao | 2023 | Journal of Environmental Sciences2023，，3： | 0 |
| 18 | Investigation of Nitrite Production Pathway in Integrated Partial Denitrification/Anammox Process via Isotope Labelling Technique and the Relevant Microbial Communities显示文摘In this study,the nitrogen removal performance of partial denitrificaiton/anammox(PDA)process was investigated by using an UASB reactor.High total nitrogen(TN)removal efficiency(91.97%)was achieved at an influent nitrogen loading rate of 0.64 kg/(m3·d).Anammox bacteria did execute the function of converting nitrate to nitrite in PDA system according to ^(15)N isotope labeling experiments and the contribution was approximately 36.3%.Candidatus_Brocadia,Candidatus_Kuenenia and Thauera were functional strains for anammox and denitrification process,respectively.Thauera and Candidatus_Brocadia were more important for TN removal at high loading rates(0.64 kg/(m3·d)).This result can provide a theoretical and technical foundation for the application of the PDA process. | Li Yanzhe Gai Jianing Zhang Xiaofei Zhao dongfeng Guo Yadong Yu Gengxing Zhao Chaocheng Liu Fang Zhao Ruiyu Liu Chunshuang | 2022 | China Petroleum Processing & Petrochemical Technology2022，24，1： | 0 |
| 19 | Preparation and Modification of Micro-Mesoporous Carbon Materials for Toluene Adsorption显示文摘In the process of toluene adsorption, the choice of adsorbent is the key. In this work, micro-mesoporous carbon materials were prepared and characterized. The synthesis process was performed by using MCM-41 as the template, and sucrose and furfuryl alcohol as the carbon source, respectively. The toluene adsorption capacity of studied adsorbents was evaluated under different toluene initial concentration, temperature and bed height. In order to further improve the adsorption capacity, nitric acid was used for modification of the selected adsorbent. The adsorption performance after modification was also investigated. The prepared carbon materials were characteristic of typical type IV isotherms and pore size distribution was focused on micropores and mesopores. Compared with MCM-41, the prepared carbon materials showed high toluene adsorption capacity. After modification the specific surface area and oxygen groups of adsorbent were increased, making the adsorption capacity increase from 185.3 mg/g to a maximum value of 514.7 mg/g. The adsorption capacity of adsorbent mainly depended on its surface area and surface chemical property. | Liu Fang Xue Ming Wang Hongxi Chai Qingwen Li Meng Dai Yexin Zhao Chaocheng Wang Yongqiang | 2018 | China Petroleum Processing & Petrochemical Technology2018，20，1： | 0 |
| 20 | Complete Nitrogen Removal through Integrating Anammox and Autotrophic Denitrification in an UASB Reactor显示文摘Complete nitrogen removal was achieved through integrating anammox and autotrophic denitrification in an UASB reactor.The total nitrogen(TN)removal rate increased stepwise from 0.46 to 0.94 kg-N/(m3·d),with an effluent TN concentration of below 3.0 mg-N/L achieved.The process is relatively insensitive to the nitrite to ammonium ratio,achieving complete nitrogen removal when their ratio in the influent varied in the range of 1.35-1.55.The added S0 quantity in the system could be utilized to adjust the competition between autotrophic denitrifiers and anammox bacteria.High-throughput sequencing technology indicated that Candidatus_Kuenenia and Thiobacillus were the functional strains for anammox and autotrphic denitrification process,respectively,in the studied reactor.This result provides a theoretical and technical basis for the large-scale application of anaerobic ammonium oxidation process. | Guo Yadong Bai Xue Yu Haitong Li Xuechen Zhao Chaocheng Liu Chunshuang | 2020 | China Petroleum Processing & Petrochemical Technology2020，22，1： | 0 |
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