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| 1 | A leaf shape mutant provides insight into PINOID Serine/Threonine Kinase function in cucumber(Cucumis sativus L.)显示文摘Optimizing leaf shape is a major challenge in efforts to develop an ideal plant type. Cucumber leaf shapes are diverse;however, the molecular regulatory mechanisms underlying leaf shape formation are unknown. In this study, we obtained a round leaf mutant(rl) from an ethyl methanesulfonate-induced mutagenesis population. Genetic analysis revealed that a single recessive gene, rl, is responsible for this mutation. A modified Mut Map analysis combined linkage mapping identified a single nucleotide polymorphism within a candidate gene,Csa1 M537400, as the mutation underlying the trait.Csa1 M537400 encodes a PINOID kinase protein involved in auxin transport. Expression of Csa1 M537400 was significantly lower in the rl mutant than in wild type, and it displayed higher levels of IAA(indole-3-acetic acid) in several tissues. Treatment of wild-type plants with an auxin transport inhibitor induced the formation of round leaves,similar to those in the rl mutant. Altered expression patterns of several auxin-related genes in the rl mutant suggest that rl plays a key role in auxin biosynthesis,transport, and response in cucumber. These findings provide insight into the molecular mechanism underlying the regulation of auxin signaling pathways in cucumber,and will be valuable in the development of an ideal plant type. | Mengfei Song Feng Cheng Jing Wang Qingzhen Wei Wenyuan Fu Xiaqing Yu Ji Li Jinfeng Chen Qunfeng Lou | 2019 | Journal of Integrative Plant Biology2019，61，9： | 3 |
| 2 | Five-year follow-up study of multi-domain cognitive training for healthy elderly community members显示文摘 | Wei FENG Chunbo LI You CHEN Yan CHENG Wenyuan WU | 2014 | 上海精神医学2014，26，1： | 3 |
| 3 | Suppression of NF-κB pathway by crocetin contributes to attenuation of lipopolysaccharide-induced acute lung injury in mice显示文摘 | Ruhui Yang Lina Yang Xiangchun Shen Wenyuan Cheng Bohua Zhao Kazi Hamid Ali Zhiyu Qian Hui Ji | 2011 | European Journal of Pharmacology2011，，2： | 1 |
| 4 | Ga-based liquid metal as an extreme pressure lubricant for steel-ceramic pairs显示文摘Extreme pressure lubrication is a crucial engineering problem for many mechanical assemblies.Herein,an extraordinary and novel functional material,Ga-based liquid metal(GLM),is found to be a promising extreme pressure lubricant for steel-ceramic sliding pairs and bears applied load above 1500 N.A Ga-rich metallic tribofilm forms on the frictional interface during sliding,providing good lubricity and bearing capability.In addition,the lubrication performance of GLM strongly depends on its composition and the contacting materials.Ternary Ga-In-Sn alloy has superior lubrication to binary Ga-In alloy.GLM lubrication is more effective on the interfaces of AISI52100/Si_(3)N_(4)than on those of AISI52100/SiC sliding pairs. | YANG DongSheng CHEN WenYuan CHEN Jiao SUN QiChun TAN Hui CHENG Jun | 2022 | Science China(Technological Sciences)2022，65，5： | 1 |
| 5 | Structural changes of a bacteriophage upon DNA packaging and maturation显示文摘Dear Editor,Tailed,double-stranded DNA(dsDNA)bacteriophages,which belong to the order of Caudovirales,have a tail attached to a pentameric vertex of the icosahedral capsid shell(head)through a 12-fold portal(Johnson and Chiu,2007).The phages package genomic dsDNA into a round procapsid using the portal in complex with an ATP-dependent terminase complex as the motor.During packaging,the procapsid shell expands to a more angular intermediate to match the size of the viral genome(Guo et al.,2014).When the phage head is full,the portal detects internal pressure and conveys a signal from the inner capsid to the exterior,which triggers a sequence of events-the terminase complex cleaving mature DNA genome from a multi-genome concatemer,the release of the terminase complex from the portal,and the attachment of the tail complex-in the completion of phage assembly(Lander et al.,2006;Johnson and Chiu,2007).At the beginning of phage infection,the tail is responsible for receptor recognition,and the portal and tail act as a tunnel for DNA delivery into the host cytoplasm(Johnson and Chiu,2007).These mechanisms of DNA packaging and ejection may also be conserved in many other DNA viruses,including herpesvirus(Wang et al.,2020;Yang et al.,2020). | Wenyuan Chen Hao Xiao Xurong Wang Shuanglin Song Zhen Han Xiaowu Li Fan Yang Li Wang Jingdong Song Hongrong Liu Lingpeng Cheng | 2020 | Protein & Cell2020，11，5： | 1 |
| 6 | Aeroelastic Tailoring of Composite Wing Structures by Laminate Layup Optimization显示文摘 | Shijun Guo Wenyuan Cheng Degang Cui | 2006 | American Institute of Aeronautics and Astronautics Journal2006，44，12： | 1 |
| 7 | Retinal Nerve Fiber Layer Thickness is Associated with Episodic Memory Deficit in Mild Cognitive Impairment Patients显示文摘 | Yuan Shen Liang Liu Yan Cheng Wei Feng Zhongyong Shi Yikang Zhu Wenyuan Wu Chunbo Li | 2014 | Current Alzheimer Research2014，，3： | 1 |
| 8 | Non-abrasive polishing of glass显示文摘 | Wu Xiaosheng Cheng Wenyuan Wang Lijiang | 2002 | Machine Tools & Manufacture2002，42，： | 1 |
| 9 | The formation mechanism of membranes prepared from the nonsolvent-solvent-crystalline polymer systems显示文摘 | Cheng Liaoping Young Taihorng Chuang Wenyuan | 2001 | Polymer2001，42，： | 1 |
| 10 | Capture-Release Strategy Facilitates Rapid Enzymatic Assembly of Oligosaccharides显示文摘A convenient and highly efficient strategy was developed for the simplification of enzymatic synthesis and purification of oligosaccharides.Glycosyl acceptors terminated with a thiol tag were extended by enzymatic modular assembly(EMA)in the aqueous phase,and the desired products were captured during solid-phase workup(SPW)using commercially available thiopropyl Sepharose 6B resin through a reversible disulfide linkage.Finally,the products were released from solid-phase resin in the presence of dithio-threitol(DTT)as reducing agent and the resin could be recovered and reused.This strategy overcomes the uncertain influence of polymer-or ionic-supports commonly used in enzymatic glycosylation,and showed perfect compatibility with all 10 enzyme modules for the rapid assembly of a series of complex oligosaccharides. | Wenyuan Fang Kan Zhong Jiansong Cheng Xian-Wei Liu Chang-Cheng Liu Zhongfu Wang Hongzhi Cao | 2022 | Chinese Journal of Chemistry2022，40，3： | 0 |
| 11 | Flotation separation of wolframite from calcite using a new trisiloxane surfactant as collector显示文摘Since wolframite is usually associated with calcite,the separation and enrichment of wolframite by froth flotation remains a great challenge.Herein,a novel trisiloxane surfactant N-(2-aminoethyl)-3-ami nopropyltrisiloxane(AATS)was successful synthesized,which was used for the separation of wolframite from calcite for the first time.The flotation separation performance of AATS was studied by flotation test,and its adsorption mechanism was explored based on contact angle,infrared spectrum analysis(FTIR),zeta potential and density functional theory(DFT)calculation.The results of microflotation test and binary mixed ore flotation test pointed that AATS had excellent selectivity and more prominent collection capacity for the flotation of wolframite when compared with industrial reagent sodium oleate(NaOL).The measurement results of contact angle proved that AATS improved the hydrophobicity of the wolframite surface.The highly selective adsorption mechanism of AATS surfactant on mineral surfaces were further researched and analyzed by FTIR and zeta potential.The results revealed that AATS surfactant had significant adsorption effect on wolframite,yet almost no adsorption on calcite.DFT calculation indicated that AATS produced electrostatic adsorption with wolframite surface through—N+H3 group. | Shuyi Shuai Zhiqiang Huang Vladimir E.Burov Vladimir Z.Poilov Fangxu Li Hongling Wang Rukuan Liu Shiyong Zhang Chen Cheng Wenyuan Li Xinyang Yu Guichun He Weng Fu | 2023 | International Journal of Mining Science and Technology2023，33，3： | 0 |
| 12 | Deformation behavior and fluid action of quartz veins in the Xuelongshan metamorphic complex,Western Yunnan显示文摘The Xuelongshan metamorphic complex underwent Cenozoic sinistral strike-slip shearing and exhumation from deep to shallow in the southeast margin of the Tibet Plateau.We studied the deformation behavior and fluid action of quartz veins in the metamorphic complex in depth based on macroscopic observation,electron backscatter diffraction fabric,and fluid inclusion analysis.The mylonitic samples are characterized by strongly elongated and orientated porphyroclasts and a finegrained matrix with a shear strain of 2.4.The ultramylonitic samples are characterized by a strongly fine-grained matrix with a shear strain of 5.8.The{m}glide controls the quartz deformation in the mylonites and ultramylonites.As the dominant recovery mechanism,subgrain rotation dynamic recrystallization reduces the grain size and promotes rheological weakening.The porphyroclasts restrict the crystal orientation of the recrystallized quartz matrix.The mylonitic quartz veins have abundant single-phase and two-phase fluid inclusions.However,the ultramylonitic quartz veins have a few fluid inclusions,most of which are small and irregular toward the grain boundaries.The temperature and pressure of fluid activity in quartz veins are 550–500℃and 391–218 MPa for ultramylonitic quartz veins,with a depth of 14.2–7.9 km and 450–400℃and 236–91 MPa for mylonitic quartz veins,with a depth of 8.6–3.3 km.The deformation process changes the shape of fluid inclusions from mylonitization to ultramylonitization and causes fluid leakage,an increase in local fluid pressure at the boundary,and hydrolysis weakening,promoting lattice dislocation glide and recrystallization,which is directly related to regional continuous shear deformation and exhumation. | Shuting WANG Shuyun CAO Lefan ZHAN Xuemei CHENG Yanlong DONG Xiaowen LI Wenyuan LI | 2023 | Science China Earth Sciences2023，66，9： | 0 |
| 13 | A mutation in CsKTN1 for the Katanin p60 protein results in miniature plant in cucumber,Cucumis sativus L.显示文摘Katanin,a microtubule shearing protein,plays an important role in plant architecture formation.However,little is known about its mechanisms in regulating plant architecture in cucumber.In the present study,through EMS mutagenesis,we identified a novel micro-plant(mp)mutant in the North China type inbred line CCMC that may be of value for cucumber breeding.The size and number of stem cells were altered in the mp mutant.Through bulked segregant analysis(BSA)sequencing approach combined with genetic mapping,the mp locus was delimited to an interval of 130.9-kb.Multiple lines of evidence suggested that the mp mutation is due to a single nucleotide polymorphism in Csa7G435510 that is predicted to encode the Katanin p60 subunit protein(CsKTN1).The expression levels of CsKTN1 decreased significantly in all tissues except the tendril of mp mutant.Subcellular localization showed that both wild-type and mutant CsKTN1 proteins were located in cell membrane,cytoplasm and nucleus of tobacco leaf cells.The mutant protein lost part of its ability to bind and shear microtubule in vitro.These findings provide new insight into the regulatory function of microtubule-shearing protein,Katanin p60,in plant architecture of cucumber. | MengFei Song WenYuan Fu YuHui Wang Feng Cheng MengRu Zhang JinFeng Chen QunFeng Lou | 2022 | Vegetable Research2022，2，1： | 0 |
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