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| 1 | De novo assembly of a new Olea europaea genome accession using nano pore seque ncing显示文摘Olive(Olea europaea L.)is internationally renowned for its high-end product,extra virgin olive oil.An incomplete genome of O.europaea was previously obtained using shotgun sequencing in 2016.To further explore the genetic and breeding utilization of olive,an updated draft genome of olive was obtained using Oxford Nanopore third-generation sequencing and Hi-C technology.Seven different assembly strategies were used to assemble the fi nal genome of 1.30 Gb,with contig and scaffold N50 sizes of4.67 Mb and 42.60 Mb,respectively.This greatly increased the quality of the olive genome.We assembled 1.1 Gb of sequences of the total olive genome to 23 pseudochromosomes by Hi-C,and 53,518 protein-coding genes were predicted in the current assembly.Comparative genomics analyses,including gene family expansion and contraction,whole-genome replication,phylogenetic analysis,and positive selection,were performed.Based on the obtained high-quality olive genome,a total of nine gene families with 202 genes were identi fi ed in the oleuropein biosynthesis pathway,which is twice the number ofgenes identi fi ed from the previous data.This new accession of the olive genome is of suf fi cient quality for genome-wide studies on gene function in olive and has provided a foundation for the molecular breeding of olive species. | Guodong Rao Jianguo Zhang Xiaoxia Liu Chunfu Lin Huaigen Xin Li Xue Chenhe Wang | 2021 | Horticulture Research2021，8，1： | 4 |
| 2 | Haploidentical hematopoietic cell transplantation for severe acquired aplastic anemia: a case-control study of post-transplant cyclophosphamide included regimen vs. anti-thymocyte globulin & colony-stimulating factor-based regimen显示文摘Dear Editor,Haploidentical allogeneic hematopoietic stem cell transplantation(haplo-HSCT),a curative therapy for severe aplastic anemia(SAA)patients,has been used clinically for decades.Two models,not involving ex vitro T-cell depletion,have been adopted for haplo-HSCT in patients with SAA.The first is referred to as the'Beijing protocol'(Xu et al.,2017),and comprises a conditioning regimen using busulfex(BU),cyclophosphamide(CY). | Lanping Xu Bin Fu Wenjing Wang Yajing Xu Depei Wu Shunqing Wang Qifa Liu Linghui Xia Sujun Gao Ming Jiang Jianmin Wang Xi Zhang Hai Bai Huiren Chen Chunfu Li Xiaojun Huang | 2020 | Science China(Life Sciences)2020，63，6： | 3 |
| 3 | Preparation and Evaluation of Insulin-loaded Nanoparticles based on Hydroxypropyl-β-cyclodextrin Modified Carboxymethyl Chitosan for Oral Delivery显示文摘Novel insulin-loaded nanoparticles based on hydroxypropyl-β-cyclodextrin modified carboxymethyl chitosan(CMC-HP-β-CD) were prepared to improve the oral bioavailability of insulin. The CMC-HP-β-CD was characterized by FT-IR spectroscopy and 1H-NMR spectra. The insulin-loaded nanoparticles were prepared through crosslinking with calcium ions, and the morphology and size of the prepared nanoparticles were characterized by transmission electron microscopy(TEM) and dynamic light scattering(DLS). Cumulative release in vitro study was performed respectively in simulated gastric medium fluid(SGF, p H=1.2), simulated intestinal fluid(SIF, p H=6.8) and simulated colonic fluid(SCF, p H=7.4). The encapsulation efficiency of insulin was up to 87.14 ± 4.32% through high-performance liquid chromatography(HPLC). Statistics indicated that only 15% of the encapsulated insulin was released from the CMC-HP-β-CD nanoparticles in 36 h in SGF, and about 50% of the insulin could be released from the nanoparticles in SIF, whereas more than 80% was released in SCF. In addition, the solution containing insulin nanoparticles could effectively reduce the blood glucose level of diabetic mice. The cytotoxicity test showed that the samples had no cytotoxicity. CMC-HP-β-CD nanoparticles are promising candidates as potential carriers in oral insulin delivery systems. | 宋豪源 马晓玲 XIONG Fuliang HONG Hui LI Chunfu LI Lianghong WU Shanshan ZHANG Xueqiong 张娟 胡建华 | 2016 | Journal of Wuhan University of Technology(Materials Science)2016，31，6： | 3 |
| 4 | A Nomogram-based Model to Predict Neoplastic Risk for Patients with Gallbladder Polyps显示文摘Background and Aims:Gallbladder polyp(GBP)assessment aims to identify the early stages of gallbladder carcinoma.Many studies have analyzed the risk factors for malignant GBPs.In this retrospective study,we aimed to establish a more accurate predictive model for potential neoplastic polyps in patients with GBPs.Methods:We devel-oped a nomogram-based model in a training cohort of 233 GBP patients.Clinical information,ultrasonographic find-ings,and blood test findings were analyzed.Mann-Whitney U test and multivariate logistic regression analyses were used to identify independent predictors and establish the nomogram model.An internal validation was conducted in 225 consecutive patients.Performance and clinical bene-fit of the model were evaluated using receiver operating characteristic curves and decision curve analysis(DCA),re-spectively.Results:Age,cholelithiasis,carcinoembryonic antigen,polyp size,and sessile shape were confirmed as independent predictors of GBP neoplastic potential in the training group.Compared with five other proposed predic-tion methods,the established nomogram model presented better discrimination of neoplastic GBPs in the training co-hort(area under the curve[AUC]:0.846)and the validation cohort(AUC:0.835).DCA demonstrated that the greatest clinical benefit was provided by the nomogram compared with the other five methods.Conclusions:Our developed preoperative nomogram model can successfully be used to evaluate the neoplastic potential of GBPs based on simple clinical variables that maybe useful for clinical decision-making. | Xudong Zhang Jincheng Wang Baoqiang Wu Tao Li Lei Jin Yong Wu Peng Gao Zhen Zhang Xihu Qin Chunfu Zhu | 2022 | Journal of Clinical and Translational Hepatology2022，10，2： | 3 |
| 5 | Effects of L-3-n-butylphthalide on caspase-3 and nuclear factor kappa-B expression in primary basal forebrain and hippocampal cultures after beta-amyloid peptide 1-42 treatment显示文摘BACKGROUND:L-3-n-butylphthalide(L-NBP) can inhibit phosphorylation of tau protein and reduce the neurotoxicity of beta-amyloid peptide 1-42(Aβ_(1-42)). OBJECTIVE:To observe the neuroprotective effects of L-NBP on caspase-3 and nuclear factor kappa-B(NF-κB) expression in a rat model of Alzheimer's disease. DESIGN,TIME AND SETTING:A cell experiment was performed at the Central Laboratory of Provincial Hospital affiliated to Shandong University between January 2008 and August 2008. MATERIALS:L-NBP(purity>98%) was provided by Shijiazhuang Pharma Group NBP Pharmaceutical Company Limited.Aβ_(1-42),3-[4,5-dimethylthiazolo-2]-2,5 iphenyltetrazolium bromide(MTT),and rabbit anti-Caspase-3 polyclonal antibody were provided by Cell Signaling, USA;goat anti-choactase and rabbit anti-NF-κB antibodies were provided by Santa Cruz,USA. METHODS:Primary cultures were generated from rat basal forebrain and hippocampal neurons at 17 or 19 days of gestation.The cells were assigned into five groups:the control group,the Aβ_(1-42) group(2μmol/ L),the Aβ_(1-42) + 0.1μmol/L L-NBP group,the Aβ_(1-42)+ 1μmol/L L-NBP group,and the Aβ_(1-42)+ 10μmol/L L-NBP group.The neurons were treated with Aβ_(1-42)(2μmol/L) alone or in combination with L-NBP(0.1,1,10μmol/L) for 48 hours.Cells in the control group were incubated in PBS. MAIN OUTCOME MEASURES:Morphologic changes were evaluated using inverted microscopy, viability using the MTT method,and the changes in caspase-3 and NF-κB expression using Western blot. RESULTS:Induction with Aβ_(1-42) for 48 hours caused cell death and soma atrophy,and increased caspase-3 and NF-κB expression(P<0.05).L-NBP blocked these changes in cell morphology, decreased caspase-3 and NF-κB expression(P<0.05),and improved cell viability,especially at the high dose(P<0.05). CONCLUSION:Aβ_(1-42) is toxic to basal forebrain and hippocampal primary neurons;L-NBP protects against this toxicity and inhibits the induction of caspase-3 and NF-κB expression. | Ruixia Wang Yong Zhang Liangliang Jiang Guozhao Ma Qingxi Fu Jialong Li Peng Yan Lunqian Shen Yabo Feng Chunxia Li Zaiying Pang Yuanxiao Cui Chunfu Chen Yifeng Du Zhaokong Liu | 2009 | Neural Regeneration Research2009，4，4： | 3 |
| 6 | SecureWeb: Protecting Sensitive Information Through the Web Browser Extension with a Security Token显示文摘The leakage of sensitive data occurs on a large scale and with increasingly serious impact. It may cause privacy disclosure or even property damage. Password leakage is one of the fundamental reasons for information leakage, and its importance is must be emphasized because users are likely to use the same passwords for different Web application accounts. Existing approaches use a password manager and encrypted Web application to protect passwords and other sensitive data; however, they may be compromised or lack accessibility. The paper presents Secure Web, which is a secure, practical, and user-controllable framework for mitigating the leakage of sensitive data. Secure Web protects users' passwords and aims to provide a unified protection solution to diverse sensitive data. The efficiency of the developed schemes is demonstrated and the results indicate that it has a low overhead and are of practical use. | Shuang Liang Yue Zhang Bo Li Xiaojie Guo Chunfu Jia Zheli Liu | 2018 | Tsinghua Science and Technology2018，23，5： | 2 |
| 7 | An Efficient Format-Preserving Encryption Mode for Practical Domains显示文摘Format-preserving encryption (FPE), which makes sure that ciphertext has the same format as plaintext, has been widely used in protecting sensitive data in a database. Aiming at efficiently solving the FPE problem on a collection of practical domains, we propose the RREM (random reference-based encryption mode), which constructs bijection between the original domain and integer set through distance computation. If an appropriate distance function is predefined, the proposed mode can solve the FPE problem on linear equidistance domain in a more efficient way than previous methods. Furthermore, we make a classification on various types of domains, show the application of RREM in some practical domains, and specify RREM's capability of solving the FPE problem on frequently-used fields in database quite efficiently. | LI Jingwei LIU Zheli XU Li JIA Chunfu | 2012 | Wuhan University Journal of Natural Sciences2012，17，5： | 2 |
| 8 | Threat Model and Defense Scheme for Side-Channel Attacks in Client-Side Deduplication显示文摘In cloud storage,client-side deduplication is widely used to reduce storage and communication costs.In client-side deduplication,if the cloud server detects that the user’s outsourced data have been stored,then clients will not need to reupload the data.However,the information on whether data need to be uploaded can be used as a side-channel,which can consequently be exploited by adversaries to compromise data privacy.In this paper,we propose a new threat model against side-channel attacks.Different from existing schemes,the adversary could learn the approximate ratio of stored chunks to unstored chunks in outsourced files,and this ratio will affect the probability that the adversary compromises the data privacy through side-channel attacks.Under this threat model,we design two defense schemes to minimize privacy leakage,both of which design interaction protocols between clients and the server during deduplication checks to reduce the probability that the adversary compromises data privacy.We analyze the security of our schemes,and evaluate their performances based on a real-world dataset.Compared with existing schemes,our schemes can better mitigate data privacy leakage and have a slightly lower communication cost. | Guanxiong Ha Hang Chen Chunfu Jia Mingyue Li | 2023 | Tsinghua Science and Technology2023，28，1： | 1 |
| 9 | Intrusion Detection Method for Program Vulnerability via Library Calls显示文摘Library function call sequence is the direct reflec- tion of a program’s behavior. The relationship between program vulnerability and library calls is analyzed, and an intrusion detection method via library calls is proposed, in which the short sequences of library call are used as signature profile. In this intrusion detection method, library interposition is used to hook library calls, and with the discussion of the features of the library call sequence in detail, an algorithm based on informa- tion-theory is applied to determine the appropriate length of the library call sequence. Experiments show good performance of our method against intrusions caused by the popular program vulnerabilities. | DUAN Xuetao ZHONG Anming LI Ying JIA Chunfu | 2007 | Wuhan University Journal of Natural Sciences2007，12，1： | 1 |
| 10 | On self-liveness of a class of Petri net models for flexible manufacturing systerns显示文摘 | Zhong Chunfu Li Zhiwu | 2009 | IET Control Theory and Application2009，，： | 1 |
| 11 | Dual‑Performance Optimized Silks from Ultra‑Low Dose Polymer Dots Feeding and Its Absorption, Distribution and Excretion in the Silkworms显示文摘Fluorescent polymer dots(Pdots)have the advantages of excellent optical properties,great biocompatibility and high photostability.Herein,we feed ultra-low doses Pdots to silkworms for the first time and aim to prepare dual-performance modified silks.After Pdots feeding,the fluorescence signal of cocoons and degummed silks increases significantly,which is more stable and more uniform than that of post-treatment silks.Moreover,Pdots hinder the conformation transformation of silk fibroin and improve the mechanical property of twisted silk strand.The highest elongation at break point is 20.75±0.03% and breaking strength is 271.7±3.8 MPa.With excellent fluorescence and mechanical properties,the optimized silks are successfully applied as a scaffold for cell culture and imaging.Furthermore,we investigate the metabolism of Pdots in the silkworms for understanding the behaviours of Pdots in the process of silks synthesis and secretion. | Jingru Li Yuqiao Li Shuting Lu Juxiang Zhang Chunfu Zhang Liqin Xiong | 2022 | Advanced Fiber Materials2022，4，4： | 1 |
| 12 | An induced OWA operator in coal mine safety evaluation 显示文摘 | WEI Chunfu PEI Zheng LI Huaming | 2012 | Journal of Computer and System Sciences2012，78，： | 1 |
| 13 | A real-time system for tracking and classification of pedestrian and bicycles 显示文摘 | Li Juan Shao Chunfu Xu Wangtu | 2010 | In Transportation Research Record: Journal of the Transportation Research Board2010，2198，： | 1 |
| 14 | 显示文摘 | Li Chunfu Dai Jialin Wang Bin | 2004 | Transaction of Materials and Heat Treatment2004，25，5： | 1 |
| 15 | Highly sensitive magnetite nano clusters for MR cell imaging显示文摘 | Mingli Li Hongchen Gu Chunfu Zhang | 2012 | Nanoscale Research Letters2012，，1： | 1 |
| 16 | An induced OWA operator in coal mine safety evaluation显示文摘 | WEI Chunfu PEI Zheng LI Huamin | 2012 | Journal of Computerand System Sciences2012，78，4： | 1 |
| 17 | Crystalline and amorphous Mn0_(2)cathodes with open framework enable high-performance aqueous zinc-ion batteries显示文摘Currently,δ-Mn0_(2)is one of the popularly studied cathode materials for aqueous zinc-ion batteries(ZIBs)but impeded by the sluggish kinetics of Zn^(2+)and the Mn cathode dissolution.Here,we report our discovery in the study of crystalline/amorphous Mn0_(2)(disordered Mn0_(2)),prepared by a simple redox reaction in the order/disorder engineering.This disordered Mn0_(2)cathode material,having open framework with more active sites and more stable structure,shows improved electrochemical performance in 2 mol L^(-1)ZnSO_(4)/0.1 mol L^(-1)MnS0_(4)aqueous electrolyte.It delivers an ultrahigh discharge specific capacity of 636 mA·h·g^(-1)at 0.1 A·g^(-1)and remains a large discharge capacity of 216 mA·h·g^(-1)even at a high current density of 1 A·g^(-1)after 400 cycles.Hence disordered Mn0_(2)could be a promising cathode material for aqueous ZIBs.The storage mechanism of the disordered Mn0_(2)electrode is also systematically investigated by structural and morphological examinations of ex situ,ultimately proving that the mechanism is the same as that of the δ-Mn0_(2)electrode.This work may pave the way for the possibility of using the order/disorder engineering to introduce novel properties in electrode materials for high-performance aqueous ZIBs. | Chunfu HUANG Cong WU Zilu ZHANG Yunyun XIE Yang LI Caihong YANG Hai WANG | 2021 | Frontiers of Materials Science2021，15，2： | 0 |
| 18 | 坏死激酶RIP1/RIP3通过特异性结合HSV-1的ICP6蛋白从而启动细胞坏死来抑制病毒在小鼠中的繁殖显示文摘2007年3月,细胞出版社正式推出《细胞-宿主与微生物》(月刊)。本刊出版的研究论文和综述文章重点关注对微生物与其宿主关系的理解。通过与作者、审稿人和科学编委的紧密合作,本刊的编辑人员以保持细胞出版社期刊一贯的高标准为使命,对该领域的研究结果予以及时报道。该杂志2015年公布的影响因子为12.328。 | Zhe Huang Su-Qin Wu 梁耀极 Xiaojuan Zhou Wanze Chen Lisheng Li Jianfeng Wu Qiuyu Zhuang Chang'an Chen Jingxian Li Chuan-Qi Zhong Weixiang Xia Rongbin Zhou Chunfu Zheng 韩家淮 | 2016 | 科学新闻2016，0，1： | 0 |
| 19 | Micro-nano structured VNb_(9)O_(25)anode with superior electronic conductivity for high-rate and long-life lithium storage显示文摘The oxygen vacancies and micro-nano structure can optimize the electron/Li+migration kinetics in anode materials for lithium batteries(LIBs).Here,porous micro-nano structured VNb_(9)O_(25)composites with rich oxygen vacancies were reasonably prepared via a facile solvothermal method combined with annealing treatment at 800℃for 30 h(VNb_(9)O_(25)-30 h).This micro-nano structure can enhance the contact of active material/electrolyte,and shorten the Li+diffusion distance.The introduction of oxygen vacancies can further boosts the intrinsic conductivity of VNb_(9)O_(25)-30 h for achieving excellent LIB performance.The as-prepared VNb_(9)O_(25)-30 h anode showed advanced rate capability with reversible capacity of 122.2 m A h g^(-1)at 4 A g^(-1),and delivered excellent capacity retention of~100%after 2000 cycles.Meanwhile,VNb_(9)O_(25)-30 h provides unexpected long-cycle life(i.e.,reversible capacity of 165.7 m A h g^(-1)at 1 A g^(-1)with a high capacity retention of 85.6%even after 8000 cycles).Additionally,coupled with the Li Fe PO4 cathode,the Li Fe PO4//VNb_(9)O_(25)-30 h full cell delivers superior LIB properties with high reversible capacities of 91.6 m A h g^(-1)at 5 C for 1000 cycles.Thus,such reasonable construction method can assist in other high-performance niobium-based oxides in LIBs. | Mingxing Liang Yongcong Huang Yuda Lin Guisheng Liang Cihui Huang Lan Chen Jiaxin Li Qian Feng Chunfu Lin Zhigao Huang | 2021 | Journal of Materials Science & Technology2021，，24： | 0 |
| 20 | PROPERTIES OF HIGHLY DISPERSED PALLADIUM CATALYSTS ON POLY N-VINYL-2-PYRROLIDONE显示文摘Four kinds of palladium catalysts dispersed on poly-N-vinyl-2-pyrrolidone were prepared by using CH3OH-NaOH,NaBH4,H2O or CH3OH-H2O as the reducing agent in the process of catalyst preparation. The catalysts were characterized by XPS,TEM,XRD and used for the hydrogenation of methyl acrylate.It was found that the valence state of palladium and distribution of palladium particles as well as the hydrogenation rate were greatly affected by the reducing agent.The best evenly dispersed palladium catalyst showing high hydrogenation activity was prepared using CH3OH-NaOH as the reducing agent. | SUN Juntan, CHEN Chunfu, LI Hong, and HE Binglin Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China | 1992 | Chinese Journal of Reactive Polymers1992，1，1： | 0 |
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