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| 1 | Arab/c/opste N^(6)-methyladenosine reader CPSF30-L recognizes FUE signals to control polyadenylation site choice in liquid-like nuclear bodies显示文摘The biological functions of the epitranscriptomic modification N^(6)-methyladenosine(m^(6)A)in plants are not fully understood.CPSF30-L is a predominant isoform of the polyadenylation factor CPSF30 and consists of CPSF30-S and an m^(6)A-binding YTH domain.Little is known about the biological roles of CPSF30-L and the molecular mechanism underlying its m^(6)A-binding function in alternative polyadenylation.Here,we charac-terized CPSF30-L as an Arabidopsis m^(6)A reader whose m^(6)A-binding function is required for the floral tran-sition and abscisic acid(ABA)response.We found that the m^(6)A-binding activity of CPSF30-L enhances the formation of liquid-like nuclear bodies,where CPSF30-L mainly recognizes m*A-modified far-upstream elements to control polyadenylation site choice.Deficiency of CPSF30-L lengthens the 3'untranslated region of three phenotypes-related transcripts,thereby accelerating their mRNA degradation and leading to late flowering and ABA hypersensitivity.Collectively,this study uncovers a new molecular mechanism for m^(6)A-driven phase separation and polyadenylation in plants. | Peizhe Song Junbo Yang Chunling Wang Qiang Lu Linqing Shi Subiding Tayier Guifang Jia | 2021 | Molecular Plant2021，14，4： | 12 |
| 2 | Genome and transcriptome of Papaver somniferum Chinese landrace CHM indicates that massive genome expansion contributes to high benzylisoquinoline alkaloid biosynthesis显示文摘Opium poppy(Papaver somniferum)is a source of morphine,codeine,and semisynthetic derivatives,including oxycodone and naltrexone.Here,we report the de novo assembly and genomic analysis of P.somniferum traditional landrace‘Chinese Herbal Medicine’.Variations between the 2.62 Gb CHM genome and that of the previously sequenced high noscapine 1(HN1)variety were also explored.Among 79,668 protein-coding genes,we functionally annotated 88.9%,compared to 68.8%reported in the HN1 genome.Gene family and 4DTv comparative analyses with three other Papaveraceae species revealed that opium poppy underwent two whole-genome duplication(WGD)events.The first of these,in ancestral Ranunculales,expanded gene families related to characteristic secondary metabolite production and disease resistance.The more recent species-specific WGD mediated by transposable elements resulted in massive genome expansion.Genes carrying structural variations and large-effect variants associated with agronomically different phenotypes between CHM and HN1 that were identified through our transcriptomic comparison of multiple organs and developmental stages can enable the development of new varieties.These genomic and transcriptomic analyses will provide a valuable resource that informs future basic and agricultural studies of the opium poppy. | Li Pei Baishi Wang Jian Ye Xiaodi Hu Lihong Fu Kui Li Zhiyu Ni Zhenlong Wang Yujie Wei Luye Shi Ying Zhang Xue Bai Mengwan Jiang Shuhui Wang Chunling Ma Shujin Li Kaihui Liu Wanshui Li Bin Cong | 2021 | Horticulture Research2021，8，1： | 4 |
| 3 | Permanence and Global Attractivity of a Discrete Nicholson's Blowflies Model with Delay显示文摘In this paper, we consider a discrete Nicholson's blowflies model with delay. By constructing suitable Lyapunov functional, a sufficient condition for the permanence and global attractivity of the system is obtained. An example together with its numerical simulation shows the feasibility of our main results. | Xiaoying CHEN Chunling SHI | 2017 | Journal of Mathematical Research with Applications2017，37，2： | 2 |
| 4 | Structural vaccinology: structure-based design of influenza A virus hemagglutinin subtype-specific subunit vaccines显示文摘There is a great need for new vaccine development against influenza A viruses due to the drawbacks of traditional vaccines that are mainly prepared using embryonated eggs.The main component of the current split influenza A virus vaccine is viral hemagglutinin(HA)which induces a strong antibody-mediated immune response.To develop a modern vaccine against influenza A viruses,the current research has been focused on the universal vaccines targeting viral M2,NP and HA proteins.Crystallographic studies have shown that HA forms a trimer embedded on the viral envelope surface,and each monomer consists of a globular head(HA1)and a“rod-like”stalk region(HA2),the latter being more conserved among different HA subtypes and being the primary target for universal vaccines.In this study,we rationally designed the HA head based on the crystal structure of the 2009-pandemic influenza A(H1N1)virus HA as a model,tested its immunogenicity in mice,solved its crystal structure and further examined its immunological characteristics.The results show that the HA globular head can be easily prepared by in vitro refolding in an E.coli expression system,which maintains its intact structure and allows for the stimulation of a strong immune response.Together with recent reports on some similar HA globular head preparations we conclude that structure-based rational design of the HA globular head can be used for subtype-specific vaccines against influenza viruses. | Chunling Xuan Yi Shi Jianxun Qi Wei Zhang Haixia Xiao George F.Gao | 2011 | Protein & Cell2011，2，12： | 2 |
| 5 | A study on crosslinking of phenolic fibers 显示文摘 | Liu Chunling Guo Quangui Shi Jingli | 2005 | Mater Chem Phys2005，90，23： | 1 |
| 6 | The Solar Upper Transition Region Imager(SUTRI)Onboard the SATech-01 Satellite显示文摘The Solar Upper Transition Region Imager(SUTRI)onboard the Space Advanced Technology demonstration satellite(SATech-01),which was launched to a Sun-synchronous orbit at a height of~500 km in 2022 July,aims to test the on-orbit performance of our newly developed Sc/Si multi-layer reflecting mirror and the 2k×2k EUV CMOS imaging camera and to take full-disk solar images at the Ne VII 46.5 nm spectral line with a filter width of~3 nm.SUTRI employs a Ritchey-Chrétien optical system with an aperture of 18 cm.The on-orbit observations show that SUTRI images have a field of view of~416×416 and a moderate spatial resolution of~8″without an image stabilization system.The normal cadence of SUTRI images is 30 s and the solar observation time is about16 hr each day because the earth eclipse time accounts for about 1/3 of SATech-01's orbit period.Approximately15 GB data is acquired each day and made available online after processing.SUTRI images are valuable as the Ne VII 46.5 nm line is formed at a temperature regime of~0.5 MK in the solar atmosphere,which has rarely been sampled by existing solar imagers.SUTRI observations will establish connections between structures in the lower solar atmosphere and corona,and advance our understanding of various types of solar activity such as flares,filament eruptions,coronal jets and coronal mass ejections. | Xianyong Bai Hui Tian Yuanyong Deng Zhanshan Wang Jianfeng Yang Xiaofeng Zhang Yonghe Zhang Runze Qi Nange Wang Yang Gao Jun Yu Chunling He Zhengxiang Shen Lun Shen Song Guo Zhenyong Hou Kaifan Ji Xingzi Bi Wei Duan Xiao Yang Jiaben Lin Ziyao Hu Qian Song Zihao Yang Yajie Chen Weidong Qiao Wei Ge Fu Li Lei Jin Jiawei He Xiaobo Chen Xiaocheng Zhu Junwang He Qi Shi Liu Liu Jinsong Li Dongxiao Xu Rui Liu Taijie Li Zhenggong Feng Yamin Wang Chengcheng Fan Shuo Liu Sifan Guo Zheng Sun Yuchuan Wu Haiyu Li Qi Yang Yuyang Ye Weichen Gu Jiali Wu Zhe Zhang Yue Yu Zeyi Ye Pengfeng Sheng Yifan Wang Wenbin Li Qiushi Huang Zhong Zhang | 2023 | Research in Astronomy and Astrophysics2023，23，6： | 1 |
| 7 | Enhanced porcine circovirus Cap protein production by Pichia pastoris with a fuzzy logic DO control based methanol/sorbitol co-feeding induction strategy显示文摘 | Jian Ding Chunling Zhang Minjie Gao Guoli Hou Kexue Liang Chunhua Li Jianping Ni Zhen Li Zhongping Shi | 2014 | Journal of Biotechnology2014，，： | 1 |
| 8 | The Permanence and Extinction of a Nonlinear Growth Rate Single-species Non-au-tonomous Dispersal Models with Time Delays显示文摘 | Chunling Shi Zhong Li Fengde Chen | 2007 | Nonlinear Analysis:Real Word Applications2007，8，5： | 1 |
| 9 | Dynamic behavior of a Logistic type equation with infinite delay显示文摘 | Cben Fengde Shi Chunling | 2006 | Acta Mathematieae Applieatae Siniea2006，22，2： | 1 |
| 10 | Extinction in a nonautonomous Lotka–Volterra competitive system with infinite delay and feedback controls显示文摘 | Chunling Shi Zhong Li Fengde Chen | 2012 | Nonlinear Analysis: Real World Applications2012，，： | 1 |
| 11 | 显示文摘 | Liu Chunling Guo Quangui Shi Jingli | 2004 | New carbon materials2004，19，： | 1 |
| 12 | Comparison Study of Chitosan/β-Cyclodextrin and Carboxymethyl Chitosan/β-Cyclodextrin Microspheres显示文摘To compare two microspheres of chitosan/β-cyclodextrin and carboxymethyl chitosan/β-cyclodextrin loaded theophylline as pulmonary sustained drug delivery carriers,the characteristics and ciliotoxicity were studied.The drug loadings of chitosan/ β-cyclodextrin and carboxymethyl chitosan/β-cyclodextrin microspheres were 21.09% and 21.42%,and the encapsulation efficiencies were 91.40% and 92.80%.The distributions of 50%(d0.5) of chitosan/β-cyclodextrin and carboxymethyl chitosan/β-cyclodextrin microspheres were 4.89 and 5.83 μm,respectively.Both microspheres showed spherical shape with smooth or wrinkled surfaces.FT-IR of chitosan/β-cyclodextrin microspheres demonstrated that theophylline had formed hydrogen bonds with chitosan and β-cyclodextrin,while for the carboxymethyl chitosan/β-cyclodextrin microspheres theophylline had interaction with carboxymethyl chitosan.The moisture absorption showed that an equilibrium was reached within 24 h.The two microspheres possessed better adaptability.In vitro release of theophylline from chitosan/ β-cyclodextrin microspheres was slower than that from carboxymethyl chitosan/β-cyclodextrin microspheres at pH 6.8. | LI Ruobao ZHAO Chunling ZHANG Weifen SHI Lihong TANG Jinbao | 2009 | Wuhan University Journal of Natural Sciences2009，14，4： | 1 |
| 13 | A study on crosslinking of phenolic fibers 显示文摘 | Liu Chunling Guo Quangui Shi Jingli | 2005 | Materials Chemistry and Physics2005，90，23： | 1 |
| 14 | Potentiation of synaptic strength and intrinsic excitability in the nucleus accumbens after 10 days of morphine withdrawal显示文摘 | Xiaobo Wu Meimei Shi Chunling Wei | | 0，，： | 1 |
| 15 | Extinction in a nonautonomous Lotka–Volterra competitive system with infinite delay and feedback controls显示文摘 | Chunling Shi Zhong Li Fengde Chen | 2012 | Nonlinear Analysis: Real World Applications2012，，5： | 1 |
| 16 | Design,Fabrication and Assembly of the Solar Upper Transition Region Imager(SUTRI)显示文摘The Solar Upper Transition Region Imager(SUTRI)focuses on the solar transition region to achieve dynamic imaging observation of the upper transition region.In this paper,we report the optical system design,mechanical design,ultrasmooth mirror manufacture and measurement,EUV multilayer film coating,prelaunch installation and calibration for the SUTRI payload at IPOE,Tongji University.Finally,the SUTRI carried by the SATech-01 satellite was successfully set to launch.All functions of this telescope were normal,and the observation results obtained in orbit were consistent with the design. | Zhanshan Wang Jun Yu Runze Qi Chunling He Zhengxiang Shen Li Jiang Weichen Gu Jiali Wu Zhe Zhang Pengfeng Sheng Yifan Wang Yue Yu Zeyi Ye JingJing Xia Yujie Xing Hongfei Jiao Bin Ma Qiushi Huang Zhong Zhang Xianyong Bai Yuanyong Deng Hui Tian Nange Wang Lun Shen Song Guo Lei Jing Xiaobo Chen Yang Gao Xiaocheng Zhu Xiaofeng Zhang Junwang He Qi Shi Shuo Liu Xingzi Bi | 2023 | Research in Astronomy and Astrophysics2023，23，9： | 0 |
| 17 | SOME NEW RESULTS ON THE PERMANENCE OF A LOGISTIC TYPE SYSTEM WITH TIME DELAYS显示文摘In this paper, we study a class of Logistic type system with both discrete delays and continuous delays. Some new criteria on the permanence of the system are established. An example shows the feasibility of our main results. | Shi Chunling | 2006 | Annals of Differential Equations2006，22，4： | 0 |
| 18 | Effects of Immunopotentiator of the Traditional Chinese Medicine on T Lymphocytes in Chicken Blood显示文摘In order to investigate the mechanism of action of immunoenhancer,the effects of the traditional Chinese medicine immunopromoter on the quantity and the transformation rates of T lymphocytes in the chicken blood were determined.Total 120 chickens were randomly assigned into three groups.The 1% and the 0.5% of the Chinese medicine immunopromoter were added to the chicken drinking water,respectively.The quantity of T lymphocytes in each group was measured by a-Naphthyl acetate esterase(ANAE) staining.The results showed that the percentages of T lymphocytes of the treatment groups were significantly higher than that of the control group(P<0.05),and the percentage of the 1% group significantly higher than that of the 0.5% group(P<0.05).In conclusion,the transformation rates of T lymphocytes showed that the Chinese medicine immunopromoter had the significant enhancing effect on the transformation rates of T lymphocytes of the treated chickens.The traditional Chinese medicine immunopromoter had the distinct function to promote the quantity and the transformation rate of T lymphocytes. | SHI Qiumei LI Chunling GAO Guisheng SHEN Ping TANG Shengling | 2008 | Journal of Northeast Agricultural University(English Edition)2008，15，2： | 0 |
| 19 | Glutamine metabolic microenvironment drives M2 macrophage polarization tomediate trastuzumab resistance in HER2-positive gastric cancer显示文摘Background:Trastuzumab is a first-line targeted therapy for human epidermal growth factor receptor-2(HER2)-positive gastric cancer.However,the inevitable occurrence of acquired trastuzumab resistance limits the drug benefit,and there is currently no effective reversal measure.Existing researches on the mechanism of trastuzumab resistance mainly focused on tumor cells themselves,while the understanding of the mechanisms of environment-mediated drug resistance is relatively lacking.This study aimed to further explore the mechanisms of trastuzumab resistance to identify strategies to promote survival in these patients.Methods:Trastuzumab-sensitive and trastuzumab-resistant HER2-positive tumor tissues and cells were collected for transcriptome sequencing.Bioinformatics were used to analyze cell subtypes,metabolic pathways,and molecular signaling pathways.Changes in microenvironmental indicators(such as macrophage,angiogenesis,and metabolism)were verified by immunofluorescence(IF)and immunohistochemical(IHC)analyses.Finally,a multi-scale agent-based model(ABM)was constructed.The effects of combination treatment were further validated in nude mice to verify these effects predicted by the ABM.Results:Based on transcriptome sequencing,molecular biology,and in vivo experiments,we found that the level of glutamine metabolism in trastuzumabresistant HER2-positive cells was increased,and glutaminase 1(GLS1)was significantly overexpressed.Meanwhile,tumor-derived GLS1 microvesicles drove M2macrophage polarization.Furthermore,angiogenesis promoted trastuzumab resistance.IHC showed high glutamine metabolism,M2 macrophage polarization,and angiogenesis in trastuzumab-resistant HER2-positive tumor tissues from patients and nudemice.Mechanistically,the cell division cycle 42(CDC42)promoted GLS1 expression in tumor cells by activating nuclear factor kappa-B(NF-κB)p65 and drove GLS1microvesicle secretion through IQmotif-containing GTPase-activating protein 1(IQGAP1).Based on the ABM and in vivo experiments,we confirmed that the combination of anti-glutamine metabolism,anti-angiogenesis,and pro-M1 polarization therapy had the best effect in reversing trastuzumab resistance in HER2-positive gastric cancer.Conclusions:This study revealed that tumor cells secrete GLS1 microvesicles via CDC42 to promote glutamine metabolism,M2 macrophage polarization,and pro-angiogenic function of macrophages,leading to acquired trastuzumab resistance in HER2-positive gastric cancer.A combination of anti-glutamine metabolism,anti-angiogenesis,and pro-M1 polarization therapy may provide a new insight into reversing trastuzumab resistance. | Xingbin Hu Zhenfeng Ma Beibei Xu Shulong Li Zhiqi Yao Bishan Liang Jiao Wang Wangjun Liao Li Lin Chunling Wang Siting Zheng Qijing Wu Qiong Huang Le Yu Fenghua Wang Min Shi | 2023 | Cancer Communications2023，43，8： | 0 |
| 20 | Gluconate 5-dehydrogenase (Ga5DH) participates in Streptococcus suis cell division显示文摘细菌的房间分割严格地在相等的女儿房间的形成被调整。这个过程被一系列空间、时间的管理者管理,并且这块地感兴趣的几个新因素最近被识别了。这里,我们在 zoonotic 病原体链球菌 suis 的房间分割报导 gluconate 5 脱氢酶(Ga5DH ) 的要求。Ga5DH 催化 5-ketogluconate 的可逆减小到 D-gluconate 并且对房间分割的地点局部性。在 S 的 Ga5DH 的删除。suis 与加入子孙的异常 septa 导致了丰满的形态学。重要增加也在房间长度被观察。这些缺点决心是在 S 的 Ga5DH 剥夺的后果。引起 FtsZ delocalization 的 suis。另外,有在 vitro 的 Ga5DH 的 FtsZ 的相互作用被蛋白质相互作用试金证实。这些结果显示 Ga5DH 可以工作在 S 期间阻止宫外的 Z 戒指的形成。suis 房间分割。 | Zhongyu Shi Chunling Xuan Huiming Han Xia Cheng Jundong Wang Youjun Feng Swaminath Srinivass Guangwen Lu George F. Gao | 2014 | Protein & Cell2014，5，10： | 0 |
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