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| 1 | Patterns and driving factors of WUE and NUE in natural forest ecosystems along the North-South Transect of Eastern China显示文摘从 2008 年 7 月,到 2008 年 8 月,从 22 种类的 72 件叶样品和在九天赋的 81 件土壤样品,森林生态系统对沿着东方中国(NSTEC ) 的纵贯的横断的南方镇定,从北方。把样品基于这些,我们学习了蔬菜水使用效率(WUE ) 和氮使用效率(NUE ) 的地理分发模式,并且分析了他们与环境因素的关系。蔬菜 WUE 和 NUE 分别地通过叶的 13C 的测量和占优势的种类的 C/N 被计算。结果出现了:(1 ) 蔬菜 WUE,从 2.13 ~ 28.67 mg C g1 H2O ,当蔬菜 NUE 显示出一个相反的趋势时,沿着 NSTEC 在代表性的森林生态系统从南方线性地增加了到北方,从北方增加到南方,从 12.92 ~ 29.60 g C g1 N 。(2 ) 蔬菜 WUE 和 NUE 被气候主导地驾驶并且显著地由土壤营养素因素影响。基于集中为 WUE 的 75.5% 变化正在回答的多重逐步的回归分析,吝啬的年度温度,土壤磷集中,和土壤氮(p < 0.001 ) 。当时,吝啬的年度降水和土壤磷集中能在蔬菜 NUE 解释 65.7% 变化(p < 0.001 ) 。而且,蔬菜 WUE 和 NUE 将被大气的氮免职严重也在氮影响浸透的生态系统。(3 ) 在沿着 NSTEC 的典型森林生态系统在蔬菜 WUE 和 NUE 之间有一种重要交易关系(p < 0.001 ) ,沿着 NSTEC 在自然森林生态系统在资源利用为植被显示平衡策略。这研究建议那个全球变化将影响森林生态系统的资源使用效率。然而,当减少时,植被能由增加缺乏资源的使用效率适应那些变化相对宽大的。但是极端影响例如重氮免职,将打破这交易机制并且把戏剧的骚乱给生态系统 biogeochemical 周期。 | SHENG Wenping REN Shujie YU Guirui FANG Huajun JIANG Chunming ZHANG Mi | 2011 | Journal of Geographical Sciences2011，21，4： | 19 |
| 2 | A rapid reporter assay for recombinant human brain natriuretic peptide(rhBNP)by GloSensor technology显示文摘Accurate determination of biological activity is essential in quality control of recombinant human brain natriuretic peptide(rhBNP). In previous study, we successfully developed a genetically modified cell line 293GCAC3-based ELISA assay for rhBNP. But ELISA procedure is still tedious, so this study was aimed to develop a rapid and simple bioassay for rhBNP using GloSensor technology, which provides a platform of flexible luciferase-based biosensors for real-time detection of signaling events in live cells, including cGMP production. A reporter cell line 293GCAGlo-G1 was constructed by transfecting pGloSensor^(TM) 40 F plasmid into 293GCAC3. The reporter assay based on 293GCAGlo-G1 showed high precision with intraassay CV being 8.3% and inter-assay CV being 14.1%; high accuracy with 80%, 100% and 120% recovery rate being 99.2%, 102.4% and 99.0% respectively; and great linearity with R^2 of linear fitting equation being 0.99. Besides, no significant difference was found in test results of reporter assay and 293GCAC3-based ELISA assay(paired t test, p=0.630). All these results suggested that the reporter assay was a viable assay for biological determination of rhBNP. | Lei Yu Xinchang Shi Chunmei Han Chunming Rao Junzhi Wang | 2018 | Journal of Pharmaceutical Analysis2018，8，5： | 11 |
| 3 | The R2R3-type MYB transcription factor MdMYB90-like is responsible for the enhanced skin color of an apple bud sport mutant显示文摘The anthocyanin content in apple skin determines its red coloration,as seen in a Fuji apple mutant.Comparative RNA-seq analysis was performed to determine differentially expressed genes at different fruit development stages between the wild-type and the skin color mutant.A novel R2R3-MYB transcription factor,MdMYB90-like,was uncovered as the key regulatory gene for enhanced coloration in the mutant.The expression of MdMYB90-like was 21.3 times higher in the mutant.MdMYB90-like regulates anthocyanin biosynthesis directly through the activation of anthocyanin biosynthesis genes and indirectly through the activation of other transcription factors that activate anthocyanin biosynthesis.MdMYB90-like bound to the promoters of both structural genes(MdCHS and MdUFGT)and other transcription factor genes(MdMYB1 and MdbHLH3)in the yeast one-hybrid system,electrophoretic mobility shift assay,and dual-luciferase assay.Transgenic analysis showed that MdMYB90-like was localized in the nucleus,and its overexpression induced the expression of other anthocyanin-related genes,including MdCHS,MdCHI,MdANS,MdUFGT,MdbHLH3,and MdMYB1.The mutant had reduced levels of DNA methylation in two regions(−1183 to−988 and−2018 to−1778)of the MdMYB90-like gene promoter,which might explain the enhanced expression of the gene and the increased anthocyanin content in the mutant apple skin. | Chao Sun Chunming Wang Wang Zhang Shuai Liu Weiyao Wang Xinyi Yu Tao Song Maxwell Yu Weichang Yu Shenchun Qu | 2021 | Horticulture Research2021，8，1： | 6 |
| 4 | Bioaugmentation of DDT-contaminated soil by dissemination of the catabolic plasmid pDOD显示文摘A plasmid transfer-mediated bioaugmentation method for the enhancement of dichlorodiphenyltrichloroethane(DDT) degradation in soil was developed using the catabolic plasmid p DOD from Sphingobacterium sp. D-6. The p DOD plasmid could be transferred to soil bacteria, such as members of Cellulomonas, to form DDT degraders and thus accelerate DDT degradation. The transfer efficiency of p DOD was affected by the donor, temperature,moisture, and soil type. Approximately 50.7% of the DDT in the contaminated field was removed 210 days after the application of Escherichia coli TG I(p DOD-gfp). The results suggested that seeding p DOD into soil is an effective bioaugmentation method for enhancing the degradation of DDT. | Chunming Gao Xiangxiang Jin Jingbei Ren Hua Fang Yunlong Yu | 2015 | Journal of Environmental Sciences2015，27，1： | 6 |
| 5 | Genome of a citrus rootstock and global DNA demethylation caused by heterografting显示文摘Grafting is an ancient technique used for plant propagation and improvement in horticultural crops for at least 1,500 years.Citrus plants,with a seed-to-seed cycle of 5–15 years,are among the fruit crops that were probably domesticated by grafting.Poncirus trifoliata,a widely used citrus rootstock,can promote early flowering,strengthen stress tolerance,and improve fruit quality via scion–rootstock interactions.Here,we report its genome assembly using PacBio sequencing.We obtained a final genome of 303 Mb with a contig N50 size of 1.17Mb and annotated 25,680 protein-coding genes.DNA methylome and transcriptome analyses indicated that the strong adaptability of P.trifoliata is likely attributable to its special epigenetic modification and expression pattern of resistance-related genes.Heterografting by using sweet orange as scion and P.trifoliata as rootstock and autografting using sweet orange as both scion and rootstock were performed to investigate the genetic effects of the rootstock.Single-base methylome analysis indicated that P.trifoliata as a rootstock caused DNA demethylation and a reduction in 24-nt small RNAs(sRNAs)in scions compared to the level observed with autografting,implying the involvement of sRNA-mediated graft-transmissible epigenetic modifications in citrus grafting.Taken together,the assembled genome for the citrus rootstock and the analysis of graft-induced epigenetic modifications provide global insights into the genetic effects of rootstock–scion interactions and grafting biology. | Yue Huang Yuantao Xu Xiaolin Jiang Huiwen Yu Huihui Jia Chunming Tan Gang Hu Yibo Hu Muhammad Junaid Rao Xiuxin Deng Qiang Xu | 2021 | Horticulture Research2021，8，1： | 4 |
| 6 | Global perspectives and future research directions for the phytoremediation of heavy metal-contaminated soil:A knowledge mapping analysis from 2001 to 2020显示文摘In total,9,552 documents were extracted from the Web of Science Core Collection and subjected to knowledge mapping and visualization analysis for the field of phytoremediation of HM-contaminated soil(PHMCS)with CiteSpace 5.7 R3 software.The results showed that(1)the number of publications increased linearly over the studied period.The top 10 countries/regions,institutions and authors contributing to this field were exhibited.(2)Keyword co-occurrence cluster analysis revealed a total of 8 clusters,including“Bioremediation,”“Arsenic,”“Biochar,”“Oxidative stress,”“Hyperaccumulation,”“EDTA,”“Arbuscular mycorrhizal fungi,”and“Environmental pollution”clusters(3)In total,334 keyword bursts were obtained,and the 25 strongest,longest duration,and newest keyboard bursts were analyzed in depth.The strongest keyword burst test showed that the hottest keywords could be divided into 7 groups,i.e.,“Plant bioremediation materials,”“HM types,”“Chelating amendments,”“Other improved strategies,”“Bioremediation characteristics,”“Risk assessment,”and“Other.”Almost half of the newest topics had emerged in the past 3 years,including“biochar,”“drought,”“health risk assessment,”“electrokinetic remediation,”“nanoparticle,”and“intercropping.”(4)In total,9 knowledge base clusters were obtained in this study.The studies of Ali et al.(2013),Blaylock et al.(1997),Huang et al.(1997),van der Ent et al.(2013),Salt et al.(1995),and Salt(1998),which had both high frequencies and the strongest burst scores,have had the most profound effects on PHMCS research.Finally,future research directions were proposed. | Kehui Liu Xiaojin Guan Chunming Li Keyi Zhao Xiaohua Yang Rongxin Fu Yi Li Fangming Yu | 2022 | Frontiers of Environmental Science & Engineering2022，16，6： | 4 |
| 7 | Exposure to blast shock waves via the ear canal induces deficits in vestibular afferent function in rats显示文摘The ears are air-filled structures that are directly impacted during blast exposure.In addition to hearing loss and tinnitus,blast victims often complain of vertigo,dizziness and unsteady posture,suggesting that blast exposure induces damage to the vestibular end organs in the inner ear.However,the underlying mechanisms remain to be elucidated.In this report,single vestibular afferent activity and the vestibuloocular reflex(VOR)were investigated before and after exposure to blast shock waves(~20 PSI)delivered into the left external ear canals of anesthetized rats.Single vestibular afferent activity was recorded from the superior branch of the left vestibular nerves of the blast-treated and control rats one day after blast exposure.Blast exposure reduced the spontaneous discharge rates of the otolith and canal afferents.Blast exposure also reduced the sensitivity of irregular canal afferents to sinusoidal head rotation at 0.5e2Hz.Blast exposure,however,resulted in few changes in the VOR responses to sinusoidal head rotation and translation.To the best of our knowledge,this is the first study that reports blast exposure-induced damage to vestibular afferents in an animal model.These results provide insights that may be helpful in developing biomarkers for early diagnosis of blast-induced vestibular deficits in military and civilian populations. | Yue Yu Jun Huang Xuehui Tang Jerome Allison David Sandlin Dalian Ding Yi Pang Chunming Zhang Tianwen Chen Nathan Yin Lan Chen William Mustain Wu Zhou Hong Zhu | 2020 | Journal of Otology2020，15，3： | 3 |
| 8 | Occurrence of avian leukosis virus subgroup J in commercial layer flocks in China显示文摘 | Binrui Xu Weixing Dong Chunming Yu Zhaoqing He Yanli Lv Yanzheng Sun Xiaoyu Feng Ning Li L. F. Lee Maoxiang Li | 2004 | Avian Pathology2004，，1： | 3 |
| 9 | Carbendazim induces a temporary change in soil bacterial community structure显示文摘The effect of carbendazim applications on the diversity and structure of a soil bacterial community was studied under field conditions using temperature gradient gel electrophoresis (TGGE) and partial sequence analysis of PCR-amplified 16S rRNA gene. After four successive introductions of carbendazim at a level of 0.94 kg active ingredient (a.i.)/ha, the genetic diversity (expressed as Shannon index, H ) decreased from 1.43 in the control to 1.29 in treated soil. This harmful effect seems to increase with the concentration of carbendazim. The value of H in the soil treated with carbendazim at 4.70 kg a.i./ha was reduced to 1.05 (P 0.05). The structure of soil bacterial community was also affected after four repeated applications of carbendazim at levels of 0.94, 1.88 and 4.70 kg a.i./ha, as seen in the relative intensities of the individual band. However, the bacterial community in carbendazim-treated soil recovered to that in the control 360 d after the first treatment. The results indicated that repeated applications of carbendazim could reduce soil microbial diversity and alter the bacterial community structure temporarily. | WANG Xiuguo, SONG Min, GAO Chunming, DONG Bin, ZHANG Qun, FANG Hua, YU Yunlong Department of Plant Protection, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China. | 2009 | Journal of Environmental Sciences2009，21，12： | 3 |
| 10 | Autonomic responses to blast overpressure can be elicited by exclusively exposing the ear in rats显示文摘Blast overpressure has become an increasing cause of brain injuries in both military and civilian populations. Though blast's direct effects on the cochlea and vestibular organs are active areas of study, little attention has been given to the ear's contribution to the overall spectrum of blast injury. Acute autonomic responses to blast exposure, including bradycardia and hypotension, can cause hypoxia and contribute to blast-induced neurotrauma. Existing literature suggests that these autonomic responses are elicited through blast impacting the thorax and lungs. We hypothesize that the unprotected ear also provides a vulnerable locus for blast to cause autonomic responses. We designed a blast generator that delivers controlled overpressure waves into the ear canal without impacting surrounding tissues in order to study the ear's specific contribution to blast injury. Anesthetized adult rats' left ears were exposed to a single blast wave ranging from 0 to 110 PSI(0-758 kPa). Blast exposed rats exhibited decreased heart rates and blood pressures with increased blast intensity, similar to results gathered using shock tubes and whole-body exposure in the literature. While rats exposed to blasts below 50 PSI(345 kPa) exhibited increased respiratory rate with increased blast intensity, some rats exposed to blasts higher than 50 PSI(345 kPa) stopped breathing immediately and ultimately died. These autonomic responses were significantly reduced in vagally denervated rats, again similar to whole-body exposure literature. These results support the hypothesis that the unprotected ear contributes to the autonomic responses to blast. | David S.Sandlin Yue Yu Jun Huang Chunming Zhang Alberto A.Arteaga John K.Lippincott Erin O.H.Peeden Ryan R.Guyton Lan Chen Laura L.S.Beneke Jerome C.Allison Hong Zhu Wu Zhou | 2018 | Journal of Otology2018，13，2： | 2 |
| 11 | Characteristics of the quasi-16-day wave in the mesosphere and lower thermosphere region as revealed by meteor radar,Aura satellite,and MERRA2 reanalysis data from 2008 to 2017显示文摘This study presents an analysis of the quasi-16-day wave(Q16DW)at three stations in the middle latitudes by using a meteor radar chain in conjunction with Aura Microwave Limb Sounder temperature data and MERRA2(Modern-Era Retrospective Analysis for Research and Applications,Version 2)reanalysis data from 2008 to 2017.The radar chain consists of three meteor radar stations located at Mohe(MH,53.5°N,122.3°E),Beijing(BJ,40.3°N,116.2°E),and Wuhan(WH,30.5°N,114.6°E).The Q16DW wave exhibits similar seasonal variation in the neutral wind and temperature,and the Q16DW amplitude is generally strong during winter and weak around summer.The Q16DW at BJ was found to have secondary enhancement around September in the zonal wind,which is rarely reported at similar latitudes.The latitudinal variations of the Q16DW in the neutral wind and temperature are quite different.The Q16DW at BJ is the most prominent in both neutral wind components among the three stations and the Q16DW amplitudes at MH and WH are comparable,whereas the wave amplitude in temperature decreases with decreasing latitude.The quasi-geostrophic refractive index squared at the three stations in the period from 2008 to 2017 was revealed.The results indicate that the Q16DW in the mesosphere and lower thermosphere(MLT)at MH has a limited contribution from the lower atmosphere.Around March and October,the Q16DW in the troposphere at BJ can propagate upward into the MLT region,whereas at WH,the contribution to the Q16DW in the MLT region is largely from the mesosphere. | Yun Gong Zheng Ma Chun Li XieDong Lv ShaoDong Zhang QiHou Zhou ChunMing Huang KaiMing Huang You Yu GuoZhu Li | 2020 | Earth and Planetary Physics2020，4，3： | 2 |
| 12 | Photodegradation of chromophoric dissolved organic matters in the water of Lake Dianchi, China显示文摘水样品从达恩奇湖被拿,在西南中国的 Yungui 高原上,并且实验被进行在湖水里模仿发色团的溶解有机物(CDOM ) 的光化学的降级特征。在不同轻条件下面的三组实验:紫外(紫外) 光,可见的光,和黑暗,被做,荧光性质,紫外吸收度,和在实验期间的溶解的器官的碳(文档) 集中的变化被分析与时间学习 CDOM 的 photodegradation 过程。结果证明那轻照耀导致了 CDOM 的重要光化学的降级,导致在开花性质,吸收度损失,在 aromaticity 的减少和平均分子的重量的变化,以及在水里的文档集中的衰落。比可见的光,紫外照耀才有更大的效果。然而,各种各样的 fluorophores 有不同敏感到一样的照耀条件,也就是说,象在低刺激波长的 fluorophore 一样的蛋白质比另外的 fluorophores 对紫外照耀更敏感,并且乐意地是更多经历相片降级。另外,可见轻照耀没在水里在文档上有重要影响,与由在实验时间期间的 5.57%59.9% 的文档集中减少。这些结果可以在达恩奇湖的水里在 CDOM 的环境行为上提供新知识。 | Yuan ZHANG | 2015 | Frontiers of Environmental Science & Engineering2015，9，4： | 2 |
| 13 | A study of traffic statistics of assembled burst traffic in optical burst switched networks显示文摘 | Yu Xiang Chen Yang Qiao Chunming | 2002 | Proceedings of SPIE Optical Networking and Communications Opticomm2002，4874，： | 1 |
| 14 | Traumatic brain injury induced by exposure to blast overpressure via ear canal显示文摘Exposure to explosive shockwave often leads to blast-induced traumatic brain injury in military and civilian populations.Unprotected ears are most often damaged following exposure to blasts.Although there is an association between tympanic membrane perforation and TBI in blast exposure victims,little is known about how and to what extent blast energy is transmitted to the central nervous system via the external ear canal.The present study investigated whether exposure to blasts directed through the ear canal causes brain injury in LongEvans rats.Animals were exposed to a single blast(0–30 pounds per square inch(psi))through the ear canal,and brain injury was evaluated by histological and behavioral outcomes at multiple time-points.Blast exposure not only caused tympanic membrane perforation but also produced substantial neuropathological changes in the brain,including increased expression of c-Fos,induction of a profound chronic neuroinflammatory response,and apoptosis of neurons.The blast-induced injury was not limited only to the brainstem most proximal to the source of the blast,but also affected the forebrain including the hippocampus,amygdala and the habenula,which are all involved in cognitive functions.Indeed,the animals exhibited long-term neurological deficits,including signs of anxiety in open field tests 2 months following blast exposure,and impaired learning and memory in an 8-arm maze 12 months following blast exposure.These results suggest that the unprotected ear canal provides a locus for blast waves to cause TBI.This study was approved by the Institutional Animal Care and Use Committee at the University of Mississippi Medical Center(Animal protocol#0932 E,approval date:September 30,2016 and 0932 F,approval date:September 27,2019). | Yang Ou Brad AClifton Jinghui Li David Sandlin Na Li Li Wu Chunming Zhang Tianwen Chen Jun Huang Yue Yu Jerome Allison Fan Fan Richard JRoman James Shaffery Wu Zhou Yi Pang Hong Zhu | 2022 | Neural Regeneration Research2022，17，1： | 1 |
| 15 | Efficient Fe based catalyst with nitrogen doped carbon material modification for propane non-oxidative dehydrogenation显示文摘Nitrogen doped carbon species(NC)was introduced into Fe based catalyst and applied into propane non-oxidative dehydrogenation.With characterizations of XRD,TEM and XPS,it was deduced that the NC modification induces the electrons transferred from NC species to Fe species,leading to electron-rich Fe species.Consequently,propane conversion and propene selectivity were increased by suppressing the by-products formation.It was also confirmed that such a modification strategy could be also extended to ones with different supports,iron precursors,and NC precursors. | Yuming Li Qiyang Zhang Xue Yu Guiling Jiang Yajun Wang Guiyuan Jiang Zhen Zhao Chunming Xu State | 2020 | Carbon Resources Conversion2020，3，1： | 1 |
| 16 | Occurrence of avian leukosis virus subgroup J in commercial layer flocks in China显示文摘 | Binrui Xu Weixing Dong Chunming Yu | 2004 | Avian Pathol2004，33，1： | 1 |
| 17 | Occurrence of avian leukosis virus subgroup J in commercial layer flocks in China显示文摘 | Binrui Xu Weixing Dong Chunming Yu etal | 2004 | Avian Pathology2004，33，1： | 1 |
| 18 | Short-term effect of increasing nitrogen deposition on CO 2 , CH 4 and N 2 O fluxes in an alpine meadow on the Qinghai-Tibetan Plateau, China显示文摘 | Chunming Jiang Guirui Yu Huajun Fang Guangmin Cao Yingnian Li | 2010 | Atmospheric Environment2010，，24： | 1 |
| 19 | A study of traffic statistics of assembled burst traffic in optical burst switched network 显示文摘 | Yu Xiang Chen Yang Qiao Chunming | 2002 | Proceedings of SPIE Optical Networking and Communications Opticomm2002，4874，： | 1 |
| 20 | Pre-research on Enhanced Heat Transfer Method for Special Vehicles at High Altitude Based on Machine Learning显示文摘The performance of an integrated thermal management system signi?cantly in?uences the stability of special-purpose vehicles;thus,enhancing the heat transfer of the radiator is of great signi?cance.Common research methods for radiators include?uid mechanics numerical simulations and experimental measurements,both of which are time-consuming and expensive.Applying the surrogate model to the analysis of the?ow and heat transfer in louvered?ns can effectively reduce the computational cost and obtain more data.A simpli?ed louvered-?n heat transfer unit was established,and computational?uid dynamics(CFD)simulations were conducted to obtain the?ow and heat transfer characteristics of the geometric structure.A three-factor and six-level orthogonal design was established with three structural parameters:angleθ,length a,and pitch L_p of the louvered?ns.The results of the orthogonal design were subjected to a range analysis,and the effects of the three parametersθ,a,and L_p on the j,f,and JF factors were obtained.Accordingly,a proxy model of the heat transfer performance for louvered?ns was established based onthe arti?cial neural network algorithm,and the model was trained with the data obtained by the orthogonal design.Finally,the?n structure with the largest JF factor was realized.Compared with the original model,the optimizedmodel improved the heat transfer factor j by 2.87%,decreased the friction factor f by 30.4%,and increased the comprehensive factor JF by 15.7%. | Chunming Li Xiaoxia Sun Hongyang Gao Yu Zhang | 2023 | Chinese Journal of Mechanical Engineering2023，36，2： | 1 |
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