|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion显示文摘The recent outbreak of coronavirus disease(COVID-19)caused by SARS-CoV-2 infection in Wuhan,China has posed a serious threat to global public health.To develop specific anti-coronavirus therapeutics and prophylactics,the molecular mechanism that underlies viral infection must first be defined.Therefore,we herein established a SARS-CoV-2 spike(S)protein-mediated cell-cell fusion assay and found that SARS-CoV-2 showed a superior plasma membrane fusion capacity compared to that of SARS-CoV.We solved the X-ray crystal structure of six-helical bundle(6-HB)core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain.We previously developed a pan-coronavirus fusion inhibitor,EK1,which targeted the HR!domain and could inhibit infection by divergent human coronaviruses tested,including SARS-CoV and MERS-CoV.Here we generated a series of lipopeptides derived from EK1 and found that EK1C4 was the most potent fusion inhibitor against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection with IC50s of 1.3 and 15.8 nM,about 241-and 149-fold more potent than the original EK1 peptide,respectively.EK1C4 was also highly effective against membrane fusion and infection of other human coronavirus pseudoviruses tested,including SARS-CoV and MERS-CoV,as well as SARSr-CoVs,and potently inhibited the replication of 5 live human coronaviruses examined,including SARS-CoV-2.Intranasal application of EK1C4 before or after challenge with HCoV-OC43 protected mice from infection,suggesting that EK1C4 could be used for prevention and treatment of infection by the currently circulating SARS-CoV-2 and other emerging SARSr-CoVs. | Shuai Xia Meiqin Liu Chao Wang Wei Xu Qiaoshuai Lan Siliang Feng Feifei Qi Linlin Bao Lanying Du Shuwen Liu Chuan Qin Fei Sun Zhengli Shi Yun Zhu Shibo Jiang Lu Lu | 2020 | Cell Research2020，30，4： | 81 |
| 2 | Expenditure and financial burden for the diagnosis and treatment of colorectal cancer in China:a hospital.based,multicenter,cross-sectional survey显示文摘Background: The increasing prevalence of colorectal cancer(CRC) in China and the paucity of information about relevant expenditure highlight the necessity of better understanding the financial burden and effect of CRC diagnosis and treatment. We performed a survey to quantify the direct medical and non-medical expenditure as well as the resulting financial burden of CRC patients in China.Methods: We conducted a multicenter, cross-sectional survey in 37 tertiary hospitals in 13 provinces across China between 2012 and 2014. Each enrolled patient was interviewed using a structured questionnaire. All expenditure data were inflated to the 2014 Chinese Yuan(CNY; 1 CNY = 0.163 USD). We quantified the overall expenditure and financial burden and by subgroup(hospital type, age at diagnosis, sex, education, occupation, insurance type, household income, clinical stage, pathologic type, and therapeutic regimen). We then performed generalized linear modeling to determine the factors associated with overall expenditure.Results: A total of 2356 patients with a mean age of 57.4 years were included, 57.1 % of whom were men; 13.9% of patients had stage I cancer; and the average previous-year household income was 54,525 CNY.The overall average direct expenditure per patient was estimated to be 67,408 CNY, and the expenditures for stage Ⅰ, Ⅱ, Ⅲ, and Ⅳ disease were 56,099 CNY, 59,952 CNY, 67,292 CNY, and 82,729 CNY, respectively. Non-medical expenditure accounted for 8.3%of the overall expenditure. The 1-year out-of-pocket expenditure of a newly diagnosed patient was 32,649 CNY, which accounted for 59.9% of their previous-year household income and caused 75.0% of families to suffer an unmanageable financial burden. Univariate analysis showed that financial burden and overall expenditure differed in almost all subgroups(P < 0.05), except for sex. Multivariate analysis showed that patients who were treated in specialized hospitals and those who were diagnosed with adenocarcinoma or diagnosed at a later stage were likely to spend more,whereas those with a lower household income and those who underwent surgery spent less(all P < 0.05).Conclusions: For patients in China, direct expenditure for the diagnosis and treatment of CRC seemed catastrophic,and non-medical expenditure was non-ignorable. The financial burden varied among subgroups, especially among patients with different clinical stages of disease, which suggests that, in China, CRC screening might be cost-effective. | Hui-Yao Huang Ju-Fang Shi Lan-Wei Guo Ya-Na Bai Xian-Zhen Liao Guo-iangLiu A-Yan Mao Jian-Song Ren Xiao-Jie Sun Xin-Yu Zhu Le Wang Bing-Bing Song Ling-Bin Du Lin Zhu Ji-Yong Gong Qi Zhou Yu-Qin Liu Rong Cao Ling Mai Li Lan Xiao-Hua Sun Ying Ren Jin-Yi Zhou Yuan-Zheng Wang Xiao Qi Pei-An Lou Dian Shi Ni Li Kai Zhang Jie He Min Dai | 2017 | Chinese Journal of Cancer2017，36，8： | 25 |
| 3 | Biofeedback therapy for chronic pelvic pain syndrome显示文摘Aim: To evaluate the efficacy of biofeedback therapy in patients with chronic pelvic pain syndrome(CPPS). Methods: From November 2001 to April 2002, patients visiting the Urological Outpatient Clinic of this Hospital were evaluated by means of the National Institute of Health Chronic Prostatitis Symptom Index (NIH-CPSI)and classified by the NIH classification standard. Sixty-two patients of CPPS category Ⅲ were involved in this study. All patients had been treated by conventional approaches such as antibiotics and alpha-blockers for more than half a year without any improvement. The expressed prostatic secretion results were as follows: WBC 5 to 9/high power field, lipid +-+++ and bacterial culture negative. Their NIH-CPSI were 12-40. All the 62 cases complained of micturitional irritation (frequency, urgency, splitted stream and sense of residual urine), 32 cases, of pain or discomfort at the testicular, penile, scrotal, pelvic or rectal region and 13 cases, of white secretion-dripping. The patients were treated by the Urostym Biofeedback equipment (Laborie Co., Canada) 5 times a week for 2 weeks with a stimulus intensity of 15 mA-23 mA and duration of 20 minutes. Results: Sixty patients were significantly improved or cured, while no significant improvement in the remaining 2. No apparent side effect was observed. The NIH-CPSI dropped to 6 to 14 with an average reduction of 21 (P<0.01). In the 60 improved cases, pain was relieved after 2-3treatment courses and other symptoms disappeared after 4-5 courses. Conclusion: Biofeedback therapy is a safe and effective treatment for CPPS. Large randomized clinical trials are needed to confirm its efficacy and to explore the mechanism of action. ( Asian J Androl 2003 Jun; 5:155-158 ) | Zhang-Qun YE, Dan CAI, Ru-Zhu LAN, Guang-Hui DU, Xiao-Yi YUAN, Zhong CHEN, Yang-Zhi MA, You-Ming HU, Gui-Yun ZENGDepartment of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China | 2003 | Asian Journal of Andrology2003，5，2： | 25 |
| 4 | Preliminary molecular epidemiology of the Staphylococcus aureus in lower respiratory tract infections: a multicenter study in China显示文摘 | LI De-zhi CHEN Yu-sheng YANG Jing-ping ZHANG Wei HU Cheng-ping LI Jia-shu MU Lan HU Ying-hui GENG Rong HU Ke CAI Shao-xi WAN Huan-ying WANG Qiu-yue WEI Li-ping DU Juan YU Qin ZHONG Xiao-ning WANG Rui-qin MA Jian-jun TIAN Gui-zhen WANG Si-qin GAO Zhan-cheng | 2011 | Chinese Medical Journal2011，，5： | 21 |
| 5 | Dynamic and Static Recrystallization Behavior of Low Carbon High Niobium Microalloyed Steel显示文摘The recrystallization behavior of a low carbon high niobium microalloyed steel was investigated using continuous and interrupted hot compression tests.The results showed that the initiation of dynamic recrystallization(DRX)could be detected from inflection in the plot of the strain hardening rate against stress regardless of the stress peak appearance.According to the Zener-Hollomon parameter equation,the activity energy of DRX(Qdef)was obtained and a new modified expression calculating Qdef was proposed in consideration of the chemical composition of experimental steel.Applying the 2% offset method,the static softening fraction was determined.The graphic representation of the softening fraction vs interruption time gave the information about the non-static recrystallization temperature(about 1000 ℃)and the relationship of precipitation-time-temperature.Static recrystallization kinetics followed the Avrami's law at high deformation temperature,and different values of the exponent m were given to illustrate the effect of niobium element on static recrystallization at different deformation temperatures. | LAN Liang yun QIU Chun lin ZHAO De-wen GAO Xiu hua DU Lin-xiu | 2011 | Journal of Iron and Steel Research(International)2011，18，1： | 16 |
| 6 | Tracing the first hematopoietic stem cell generation in human embryo by single-cell RNA sequencing显示文摘Tracing the emergence of the first hematopoietic stem cells(HSCs)in human embryos,particularly the scarce and transient precursors thereof,is so far challenging,largely due to the technical limitations and the material rarity.Here,using single-cell RNA sequencing,we constructed the first genome-scale gene expression landscape covering the entire course of endothelial-to-HSC transition during human embryogenesis.The transcriptomically defined HSC-primed hemogenic endothelial cells(HECs)were captured at Carnegie stage(CS)12-14 in an unbiased way,showing an unambiguous feature of arterial endothelial cells(ECs)with the up-regulation of RUNX1,MYB and ANGPTL Importantly,subcategorizing CD34+CD45-ECs into a CD44+population strikingly enriched HECs by over 10-fold.We further mapped the developmental path from arterial ECs via HSC-primed HECs to hematopoietic stem progenitor cells,and revealed a distinct expression pattern of genes that were transiently over-represented upon the hemogenic fate choice of arterial ECs#including EMCN,PROCR and RUNX1T1.We also uncovered another temporally and molecularly distinct intra-embryonic HEC population,which was detected mainly at earlier CS 10 and lacked the arterial feature.Finally,we revealed the cellular components of the putative aortic niche and potential cellular interactions acting on the HSCprimed HECs.The cellular and molecular programs that underlie the generation of the first HSCs from HECs in human embryos,together with the ability to distinguish the HSC-primed HECs from others,will shed light on the strategies for the production of clinically useful HSCs from pluripotent stem cells. | Yang Zeng Jian He Zhijie Bai Zongcheng Li Yandong Gong Chen Liu Yanli Ni Junjie Du Chunyu Ma Lihong Bian Yu Lan Bing Liu | 2019 | Cell Research2019，29，11： | 14 |
| 7 | Drug-resistant genes carried by Acinetobacter baumanii isolated from patients with lower respiratory tract infection显示文摘 | DAI Ning LI De-zhi CHEN Ji-chao CHEN Yu-sheng GENG Rong HU Ying-hui YANG Jing-ping DU Juan HU Cheng-ping ZHANG Wei LI Jia-shu YU Qin WAN Huan-ying MU Lan ZHONG Xiao-ning WEI Li-ping MA Jian-jun WANG Qiu-yue HU Ke TIAN Gui-zhen CAI Shao-xi WANG Rui-qin HE Bei WANG Si-qin WANG Zhan-wei ZHAO Su-rui GAO Zhan-cheng | 2010 | Chinese Medical Journal2010，，18： | 13 |
| 8 | Management of granulomatous lobular mastitis: an international multidisciplinary consensus(2021 edition)显示文摘Granulomatous lobular mastitis(GLM) is a rare and chronic benign inflammatory disease of the breast. Difficulties exist in the management of GLM for many front-line surgeons and medical specialists who care for patients with inflammatory disorders of the breast. This consensus is summarized to establish evidence-based recommendations for the management of GLM. Literature was reviewed using PubMed from January 1, 1971 to July 31, 2020. Sixty-six international experienced multidisciplinary experts from 11 countries or regions were invited to review the evidence.Levels of evidence were determined using the American College of Physicians grading system, and recommendations were discussed until consensus. Experts discussed and concluded 30 recommendations on historical definitions,etiology and predisposing factors, diagnosis criteria, treatment, clinical stages, relapse and recurrence of GLM. GLM was recommended as a widely accepted definition. In addition, this consensus introduced a new clinical stages and management algorithm for GLM to provide individual treatment strategies. In conclusion, diagnosis of GLM depends on a combination of history, clinical manifestations, imaging examinations, laboratory examinations and pathology.The approach to treatment of GLM should be applied according to the different clinical stage of GLM. This evidencebased consensus would be valuable to assist front-line surgeons and medical specialists in the optimal management of GLM. | Qian-Qian Yuan Shu-Yuan Xiao Omar Farouk Yu-Tang Du Fereshte Sheybani Qing Ting Tan Sami Akbulut Kenan Cetin Afsaneh Alikhassi Rami Jalal Yaghan Irmak Durur-Subasi Fatih Altintoprak Tae Ik Eom Fatih Alper Mustafa Hasbahceci David Martínez-Ramos Pelin Seher Oztekin Ava Kwong Cedric W.Pluguez-Turul Kirstyn EBrownson Shirish Chandanwale Mehran Habib Liu-Yi Lan Rui Zhou Xian-Tao Zeng Jiao Bai Jun-Wen Bai Qiong-Rong Chen Xing Chen Xiao-Ming Zha Wen-Jie Dai Zhi-Jun Dai Qin-Yu Feng Qing-Jun Gao Run-Fang Gao Bao-San Han Jin-Xuan Hou Wei Hou Hai-Ying Liao Hong Luo Zheng-Ren Liu Jing-Hua Lu Bin Luo Xiao-Peng Ma Jun Qian Jian-Yong Qin Wei Wei Gang Wei Li-Ying Xu Hui-Chao Xue Hua-Wei Yang Wei-Ge Yang Chao-Jie Zhang Fan Zhang Guan-Xin Zhang Shao-Kun Zhang Shu-Qun Zhang Ye-Qiang Zhang Yue-Peng Zhang Sheng-Chu Zhang Dai-Wei Zhao Xiang-Min Zheng Le-Wei Zheng Gao-Ran Xu Wen-Bo Zhou Gao-Song Wu | 2022 | Military Medical Research2022，9，4： | 13 |
| 9 | Target classification with low-resolution radar based on dispersion situations of eigenvalue spectra显示文摘Most low-resolution radar systems, especially ground surveillance radar systems, work at relatively low pulse repeat frequency (PRF) and with short time-on-target (TOT) (duration in scanning). Low PRF leads to Doppler ambiguity and short TOT results in low Doppler resolution, which poses a problem to target classification with low-resolution radar based on the jet engine modulation (JEM) characteristic of radar echo. From the pattern classification viewpoint, we propose a method of using dispersion situations of JEM eigenvalue spectra to categorize aeroplanes into three kinds, namely turbojet aircraft, prop aircraft and helicopter. We analyze the mathematical model of JEM echoes consisting of a series of line spectra and regard them as a sum of several series of harmonious waves. Classification features can be extracted based on the harmonious wave sum model. Some schemes for extracting features from echoes within or between pulses are proposed. Low-dimensional features are extracted to reduce computation burden. Our methods do not compensate for the fuselage echoes and are insensitive to the variation of fuselage Doppler. The feasibility of our methods is demonstrated by simulation experiment. | CHEN Feng, LIU HongWei, DU Lan & BAO Zheng National Laboratory of Radar Signal Processing, Xidian University, Xi’an 710071, China | 2010 | Science China(Information Sciences)2010，53，7： | 12 |
| 10 | Protective effect of stilbenes containing extract-fraction from Cajanus cajan L.on amyloid beta-peptide 25-35 induced cognitive deficits in mice显示文摘 | Can-Jun Ruan Lan Sun Li Zhang Jian-Yong Si Di-Hua Chen Guan-Hua Du | 2009 | 中国药理通讯2009，26，2： | 10 |
| 11 | Determination of Lignin in Marine Sediment Using Alkaline Cupric Oxide Oxidation-Solid Phase Extraction-on-Column Derivatization-Gas Chromatography显示文摘Lignin serves as one of the most important molecular fossils for tracing Terrestrial Organic Matters (TOMs) in marine environment. Extraction and derivatization of lignin oxidation products (LOPs) are crucial for accurate quantification of lignin in marine sediment. Here we report a modification of the conventional alkaline cupric oxide (CuO) oxidation method, the modification consisting in a solid phase extraction (SPE) and a novel on-column derivatization being employed for better efficiency and reproducibility. In spiking blanks, recoveries with SPE for the LOPs are between 77.84% and 99.57% with relative standard deviations (RSDs) ranging from 0.57% to 8.04% (n=3), while those with traditional liquid-liquid extraction (LLE) are from 44.52% to 86.16% with RSDs being from 0.53% to 13.14% (n=3). Moreover, the reproducibility is greatly improved with SPE, with less solvent consumption and shorter processing time. The average efficiency of on-column derivatization for LOPs is 100.8% ± 0.68%, which is significantly higher than those of in-vial or in-syringe derivatization, thus resulting in still less consumption of derivatizing reagents. Lignin in the surface sediments sampled from the south of Yangtze River estuary, China, was determined with the established method. Recoveries of 72.66% to 85.99% with standard deviation less than 0.01mg/10g dry weight are obtained except for p-hydroxyben-zaldehyde. The lignin content Σ8 (produced from 10 g dry sediment) in the research area is between 0.231 and 0.587 mg. S/V and C/V ratios (1.028 ± 0.433 and 0.192 ± 0.066, respectively) indicate that the TOMs in this region are originated from a mixture of woody and nonwoody angiosperm plants; the high values of (Ad/Al) v suggest that the TOMs has been highly degraded. | ZHANG Ting LI Xianguo SUN Shuwen LAN Haiqing DU Peirui WANG Min | 2013 | Journal of Ocean University of China2013，12，1： | 10 |
| 12 | Concurrent chemoradiotherapy with weekly docetaxel versus cisplatin in the treatment of locoregionally advanced nasopharyngeal carcinoma:a propensity score-matched analysis显示文摘Background:Promising efficacy and manageable toxicity of docetaxel-based concurrent chemoradiotherapy(CCRT)were reported in head and neck cancer.In addition,the effect of CCRT in combination with cisplatin and/or 5-fluorouracil on both locoregionally advanced and metastatic/recurrent nasopharyngeal carcinoma(NPC)was verified.However,CCRT with docetaxel for locoregionally advanced NPC are not well studied.This study aimed to compare effectiveness and toxicities of CCRT with weekly docetaxel versus tri-weekly cisplatin for locoregionally advanced NPC.Methods:Clinical data of patients with locoregionally advanced NPC newly diagnosed between January 2010 and December 2014 receiving CCRT with either weekly docetaxel(15 mg/m2)or tri-weekly cisplatin(80-100 mg/m2)were reviewed.Propensity score matching at a 1:1 ratio was performed to balance baseline characteristics.Adverse events and survival were compared between the two groups.Results:A total of 962 patients were included as the whole cohort,and 448 patients were matched and were regarded as the matched cohort.The median follow-up duration was 48 months for the whole cohort.The 3-year nodal recurrence-free survival rate was significantly increased for patients treated with docetaxel in both the whole(hazard ratio[HR]=0.37,95%confidence interval[CI]0.19-0.72,P=0.030)and matched cohorts(HR=0.33,95%CI 0.14-0.79,P=0.023).However,no significant differences were observed in overall survival,local recurrence-free survival,and distant metastasis-free survival between the two groups in both cohorts.Significantly higher rates of grade 3 radiodermatitis(6.7%vs.1.8%,P=0.001),mucositis(74.5%vs.37.9%,P<0.001),and leucopenia(2.2%vs.11.6%,P<0.001)were observed in the docetaxel group,but any grade of renal injury(1.8%vs.15.1%,P<0.001),vomiting(18.8%vs.88.3%,P<0.001),and ALT elevation(19.2%vs.31.3%,P=0.027)were more common in the cisplatin group.Conclusions:CCRT with weekly low-dose docetaxel is an effective and tolerable therapeutic regimen for locally advanced NPC.It provides a survival benefit mainly by improving the control of regional lymph node metastases,especially for patients with low pretreatment EBV DNA levels. | Jun-Fang Liao Qun Zhang Xiao-Jing Du Mei Lan Shan Liu Yun-Fei Xia Xiu-Yu Cai Wei Luo | 2019 | Cancer Communications2019，39，1： | 9 |
| 13 | Radar automatic target recognition based on feature extraction for complex HRRP显示文摘Radar high-resolution range profile(HRRP) has received intensive attention from the radar automatic target recognition(RATR) community.Usually,since the initial phase of a complex HRRP is strongly sensitive to target position variation,which is referred to as the initial phase sensitivity in this paper,only the amplitude informa-tion in the complex HRRP,called the real HRRP in this paper,is used for RATR,whereas the phase information is discarded.However,the remaining phase infor-mation except for initial phases in the complex HRRP also contains valuable target discriminant information.This paper proposes a novel feature extraction method for the complex HRRP.The extracted complex feature vector,referred to as the complex feature vector with difference phases,contains the difference phase in-formation between range cells but no initial phase information in the complex HRRP.According to the scattering center model,the physical mechanism of the proposed complex feature vector is similar to that of the real HRRP,except for reserving some phase information independent of the initial phase in the complex HRRP.The recognition algorithms,frame-template establishment methods and preprocessing methods used in the real HRRP-based RATR can also be applied to the proposed complex feature vector-based RATR.Moreover,the components in the complex feature vector with difference phases approximate to follow Gaussian distribution,which make it simple to perform the statistical recognition by such complex feature vector.The recognition experiments based on measured data show that the pro-posed complex feature vector can obtain better recognition performance than the real HRRP if only the cell interval parameters are properly selected. | DU Lan LIU HongWei BAO Zheng ZHANG JunYing | 2008 | Science in China(Series F)2008，51，8： | 9 |
| 14 | Robust radar automatic target recognition algorithm based on HRRP signature显示文摘自动目标识别(ATR ) 是为现代雷达的重要功能。目标的高分辨率范围侧面(HRRP ) 包含目标结构签名,例如目标尺寸, scatterer 分发,等等,它是为 ATR 的有希望的签名。目标 HRRP 的统计建模是为 HRRP 统计识别的关键阶段,包括模型选择和参数评价。为统计识别算法,测试样品作为训练数据的跟随一样的分发模型,这通常被假定。因为收到的 HRRP 的 signal-to-noise 比率(SNR ) 是目标距离的功能,假设不能在实践被遇见。在这份报纸,我们在场为 HRRP 统计识别的一个柔韧的方法测试 HRRP 的 SNR 什么时候比训练的低,取样。噪音是散布的假定独立 Gaussian,当 HRRP 被概率的主要部件分析(PPCA ) 建模时模型。模仿的实验基于测量数据显示出建议方法的有效性。 | Hongwei LIU Feng CHEN Lan DU Zheng BAO | 2012 | Frontiers of Electrical and Electronic Engineering in China2012，7，1： | 8 |
| 15 | Receptome profiling identifies KREMEN1 and ASGR1 as alternative functional receptors of SARS-CoV-2显示文摘Host cellular receptors play key roles in the determination of virus tropism and pathogenesis.However,little is known about SARS-CoV-2 host receptors with the exception of ACE2.Furthermore,ACE2 alone cannot explain the multi-organ tropism of SARS-CoV-2 nor the clinical differences between SARS-CoV-2 and SARS-CoV,suggesting the involvement of other receptor(s).Here,we performed genomic receptor profiling to screen 5054 human membrane proteins individually for interaction with the SARS-CoV-2 capsid spike(S)protein.Twelve proteins,including ACE2,ASGR1,and KREMEN1,were identified with diverse S-binding affinities and patterns.ASGR1 or KREMEN1 is sufficient for the entry of SARS-CoV-2 but not SARS-CoV in vitro and in vivo.SARS-CoV-2 utilizes distinct ACE2/ASGR1/KREMEN1(ASK)receptor combinations to enter different cell types,and the expression of ASK together displays a markedly stronger correlation with virus susceptibility than that of any individual receptor at both the cell and tissue levels.The cocktail of ASK-related neutralizing antibodies provides the most substantial blockage of SARS-CoV-2 infection in human lung organoids when compared to individual antibodies.Our study revealed an interacting host receptome of SARS-CoV-2,and identified ASGR1 and KREMEN1 as alternative functional receptors that play essential roles in ACE2-independent virus entry,providing insight into SARS-CoV-2 tropism and pathogenesis,as well as a community resource and potential therapeutic strategies for further COVID-19 investigations. | Yunqing Gu Jun Cao Xinyu Zhang Hai Gao Yuyan Wang Jia Wang Juan He Xiaoyi Jiang Jinlan Zhang Guanghui Shen Jie Yang Xichen Zheng Gaowei Hu Yuanfei Zhu Shujuan Du Yunkai Zhu Rong Zhang Jianqing Xu Fei Lan Di Qu Guoliang Xu Yun Zhao Dong Gao Youhua Xie Min Luo Zhigang Lu | 2022 | Cell Research2022，32，1： | 8 |
| 16 | Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors显示文摘Achievement of immunocompetent and therapeutic T lymphopoiesis from pluripotent stem cells(PSCs)is a central aim in T cell regenerative medicine.To date,preferentially reconstituting T lymphopoiesis in vivo from PSCs remains a practical challenge.Here we documented that synergistic and transient expression of Runx1 and Hoxa9 restricted in the time window of endothelial-to-hematopoietic transition and hematopoietic maturation stages in a PSC differentiation scheme(iR9-PSC)in vitro induced preferential generation of engraftable hematopoietic progenitors capable of homing to thymus and developing into mature T cells in primary and secondary immunodeficient recipients.Single-cell transcriptome and functional analyses illustrated the cellular trajectory of T lineage induction from PSCs,unveiling the T-lineage specification determined at as early as hemogenic endothelial cell stage and identifying the bona fide pre-thymic progenitors.The induced T cells distributed normally in central and peripheral lymphoid organs and exhibited abundant TCRαβrepertoire.The regenerative T lymphopoiesis restored immune surveillance in immunodeficient mice.Furthermore,gene-edited iR9-PSCs produced tumor-specific T cells in vivo that effectively eradicated tumor cells.This study provides insight into universal generation of functional and therapeutic T cells from the unlimited and editable PSC source. | Rongqun Guo Fangxiao Hu Qitong Weng Cui Lv Hongling Wu Lijuan Liu Zongcheng Li Yang Zeng Zhijie Bai Mengyun Zhang Yuting Liu Xiaofei Liu Chengxiang Xia Tongjie Wang Peiqing Zhou Kaitao Wang Yong Dong Yuxuan Luo Xiangzhong Zhang Yuxian Guan Yang Geng Juan Du Yangqiu Li Yu Lan Jiekai Chen Bing Liu Jinyong Wang | 2020 | Cell Research2020，30，1： | 7 |
| 17 | Surface enhanced Raman spectroscopy signals of mixed pesticides and their identification显示文摘A rapid and sensitive method to identify and analyze mixed pesticides of tricyclazole,paraquat and flusilazole by surface-enhanced Raman scattering has been reported.Aqueous samples can be detected by SERS in low concentrations of 0.01 mg/L, 0.1 mg/L and 2.85 mg/L for individual tricyclazole,paraquat and flusilazole respectively.When mixing the three pesticides in the low concentrations,their characteristic peaks can still be identified from the SERS spectrum of the mixture. | Hui Rong Tang Qing Qing Li Yu Lan Ren Jin Pei Geng Peng Cao Tao Sui Xuan Wang Yi Ping Du | 2011 | Chinese Chemical Letters2011，22，12： | 7 |
| 18 | Medical expenditure for esophageal cancer in China:a 10-year multicenter retrospective survey(2002-2011)显示文摘Background: Esophageal cancer is associated with substantial disease burden in China, and data on the economic burden are fundamental for setting priorities in cancer interventions. The medical expenditure for the diagnosis and treatment of esophageal cancer in China has not been fully quantified. This study aimed to examine the medical expenditure of Chinese patients with esophageal cancer and the associated trends.Methods: From 2012 to 2014, a hospital-based multicenter retrospective survey was conducted in 37 hospitals in 13 provinces/municipalities across China as a part of the Cancer Screening Program of Urban China. For each esophageal cancer patient diagnosed between 2002 and 2011, clinical information and expense data were extracted by using structured questionnaires. All expense data were reported in Chinese Yuan(CNY; 1 CNY = 0.155 USD) based on the2011 value and inflated using the year-specific health care consumer price index for China.Results: A total of 14,967 esophageal cancer patients were included in the analysis. It was estimated that the overall average expenditure per patient was 38,666 CNY, and an average annual increase of 6.27% was observed from 2002(25,111 CNY) to 2011(46,124 CNY). The average expenditures were 34,460 CNY for stage Ⅰ,39,302 CNY for stage Ⅱ,40,353 CNY for stage Ⅲ, and 37,432 CNY for stage IV diseases(P < 0.01). The expenditure also differed by the therapy type, which was 38,492 CNY for surgery, 27,933 CNY for radiotherapy, and 27,805 CNY for chemotherapy(P < 0.05).Drugs contributed to 45.02% of the overall expenditure.Conclusions: These conservative estimates suggested that medical expenditures for esophageal cancer in China substantially increased in the last 10 years, treatment for early-stage esophageal cancer costs less than that for advanced cases, and spending on drugs continued to account for a considerable proportion of the overall expenditure. | Lan-Wei Guo Hui-Yao Huang Ju-Fang Shi Li-Hong Lv Ya-Na Bai A-Yan Mao Xian-Zhen Liao Guo-Xiang Liu Jian-Song Ren Xiao-Jie Sun Xin-Yu Zhu Jin-Yi Zhou Ji-Yong Gong Qi Zhou Lin Zhu Yu-Qin Liu Bing-Bing Song Ling-Bin Du Xiao-Jing Xing Pei-An Lou Xiao-Hua Sun Xiao Qi Shou-Ling Wu Rong Cao Li Lan Ying Ren Kai Zhang Jie He Jian.Gong Zhang Min Dai | 2017 | Chinese Journal of Cancer2017，36，11： | 7 |
| 19 | Traditional Chinese Medicine syndrome-related herbal prescriptions in treatment of malignant tumors显示文摘OBJECTIVE: To investigate the distribution characteristics of TCM syndromes and the related herbal prescriptions for malignant tumors (MT). METHODS: A clinical database of the TCM syndromes and the herbal prescriptions in treatment of 136 MT patients were established. The data were then analyzed using cluster and frequency analysis. RESULTS: According to the cluster analysis, the TCM syndromes in MT patients mainly included two patterns: deficiency of both Qi and Yin and internal accumulation of toxic heat. The commonly-prescribed herbs were Huangqi (Astraglus), Nüzhen-zi (Fructus Ligustri Lucidi), Lingzhi (Ganoderma Lucidum), Huaishan (Dioscorea Opposita), Xiakucao (Prunella Vulgaris), and Baihuasheshecao (Herba Hedyotidis). CONCLUSION: Deficiency of Qi and Yin is the primary syndrome of MT, and internal accumulation of toxic heat is the secondary syndrome. The herbs for Qi supplementation and Yin nourishment are mainly used, with the assistance of herbs for heat-clearance and detoxification. | Zhizhen Liu Songyi Chen Jing Cai Enda Zhang Lan Lan Junting Zheng Lianming Liao Xuemei Yang Changen Zhou Jian Du | 2013 | Journal of Traditional Chinese Medicine2013，33，1： | 7 |
| 20 | Urbanization effect on precipitation over the Pearl River Delta based on CMORPH data显示文摘Based on the satellite data from the Climate Prediction Center morphing(CMORPH) at very high spatial and temporal resolution, the effects of urbanization on precipitation were assessed over the Pearl River Delta(PRD) metropolitan regions of China. CMORPH data well estimates the precipitation features over the PRD. Compared to the surrounding rural areas, the PRD urban areas experience fewer and shorter precipitation events with a lower precipitation frequency(ratio of rainy hours, about 3 days per year less); however, short-duration heavy rain events play a more significant role over the PRD urban areas. Afternoon precipitation is much more pronounced over the PRD urban areas than the surrounding rural areas, which is probably because of the increase in short-duration heavy rain over urban areas. | CHEN Sheng LI Wei-Biao DU Yao-Dong MAO Cheng-Yan ZHANG Lan | 2015 | Advances in Climate Change Research2015，6，1： | 6 |
您所在的机构暂未开通此部分的功能，请联系管理员