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| 1 | Immunoregulatory effects of Tripterygium wilfordii Hook Fand its extracts in clinical practice显示文摘Tripterygium wilfordii Hook F (TwHF) and its extracts have long been used for the treatment of rheumatoid arthritis, autoimmune diseases, and kidney disease due to their anti-inflammatory, immunoregulatory, and other pharmacological effects. However, the clinical immunoregulatory effects of TwHF and its extracts remain unclear, so we reviewed their effects for use in clinical practice. This review provides a comprehensive summary of the recent literature on the immunoregulatory effects of TwHF and its extracts in clinical studies. TwHF and its extracts affect the proliferation and activation of T and B cells;ratio of T cell subsets;inflammatory response of monocytes, macrophages, and immunoglobulins;and secretion of many cytokines. Together, these effects dictate immune function in a variety of diseases. TwHF and its extracts can be used alone or in combination with existing therapies against many immune disorders through immunomodulation. | Dan Luo Zhengyun Zuo Hongyan Zhao Yong Tan Cheng Xiao | 2019 | Frontiers of Medicine2019，13，5： | 19 |
| 2 | Growth-inhibiting effects of taxol on human liver cancer in vitro and in nude mice显示文摘AIM To investigate the effects of taxol onSMMC-7721 human hepatoma and itsmechanisms.METHODS In vitro cell growth was assessedby trypan blue exclusion method.Experimentalhepatoma model was established by seedingSMMC-7721 cells subcutaneously into Balb/c(nu/nu)nude mice.In vivo tumor growth wasdetermined by measurement of tumor diameterwith Vernier calipers.The syntheses of DNA,RNA and protein were analyzed by incorporationof ~3H-thymidine,~3H-uridine and ~3H-leucinerespectively.Using light and electronmicroscopes to observe the morphologicalchanges of cells including mitosis andapoptosis.RESULTS Taxol was effective against SMMC-7721 human hepatoma cell growth in the rangesof 2.5 nmol/L-10 nmol/L with mitotic arrestand apoptosis in vitro.DNA,RNA and proteinsyntheses in cells were also obviouslysuppressed by in vitro treatment of taxol for72 h.Taxol at 2.5 nmol/L reduced ~3H-thymidineuptake to about 34% of the control value(P<0.05).Increasing the dose of taxol to20 nmol/L resulted in a greater decrease in ~3H-thymidine incorporation to 60% of the controlvalue(P<0.01).At a concentration of 20 nmol/L,the ~3H-uridine and ~3H-leucine uptakeswere reduced to 52%(P<0.05)and 63%(P<0.01),respectively.In vivo,taxolsignificantly inhibited SMMC-7721 tumor growthat 10 mg/kg,i.p.,once daily for 10 d.A morethan 90% decrease in tumor volume wasobserved by day 11(P<0.01)similarly withmitotic arrest and cell apoptosis.CONCLUSION Taxol has a marked anticanceractivity in SMMC-7721 human hepatoma both invitro and in nude mice.Its mechanisms might beassociated with mitotic arrest,subsequently,apoptosis of the hepatoma cells.No obvioustoxicity was observed with in vivoadministration of taxoi. | Jin Hui Yuan Ru Ping Zhang Ru Gang Zhang Li Xia Guo Xing Wang Wang Dan Luo Yong Xie Hong Xie | 2000 | World Journal of Gastroenterology2000，6，2： | 18 |
| 3 | NK4基因转染对前列腺癌DU145细胞增殖、迁移、侵袭和凋亡的影响显示文摘研究HGF拮抗剂NK4在前列腺癌中的作用。将包含NK4cDNA的表达载体pBudCE4.1-EGFP-NK4转染到DU145细胞中。体外实验检测白分泌的NK4对肿瘤细胞增殖、迁移、转移及凋亡的影响。体内实验裸鼠分为三组,分别皮下种植DUl45、空质粒转染的Dul45和NK4转染的DUl45细胞,检测皮下肿瘤的大小、细胞凋亡及细胞增殖情况。体外实验结果显示转染NK4的DUl45细胞可以分泌NK4蛋白。自分泌的NK4抑制HGF诱导的肿瘤细胞增殖、迁移及转移,促进凋亡(P〈0.01)。NK4可以调节HGF受体c-Met及其下游ERKl与Akt1/2蛋白的活性。体内实验显示,NK4转染DUl45细胞组的肿瘤生长及细胞增殖受到抑制,同时肿瘤细胞凋亡增加。本实验显示包含NK4cDNA的表达载体转染前列腺癌细胞可以有效地调节肿瘤细胞的增殖、迁移、侵袭及凋亡。NK4作用于HGF/c-Met可以作为前列腺癌治疗的一个有效靶点。 | Dan Yue Yong Wang Ping Ma Yin-Yan Li Hong Chen Ping Wang Chang-Shan Ren | 2010 | Asian Journal of Andrology2010，12，3： | 14 |
| 4 | A cotton gene encoding a plasma membrane aquaporin is involved in seedling development and in response to drought stress显示文摘棉花(Gossypium hirsutum ) ,世界范围的最重要的纺织品庄稼,经常在它的生长期间遇到象干旱和涝灾那样的不能生活的应力季节(夏天) ,和它的生产率被不利因素显著地限制。为了调查这的分子的改编机制,种种类到不能生活的应力,编码血浆膜的基因内在的蛋白质(果仁) 在棉花被孤立,并且指定了为 GhPIP2; 7。量的反向的 transcriptase 聚合酶链反应分析显示了那 GhPIP2; 7 优先地在子叶和叶子被表示,并且它的表示在在干旱处理以后的叶子是起来调整的。GhPIP2 驾驶的 GUS 基因的强壮的表示; 7 倡导者在 5- 的叶子被检测到 10-day-old 当幼苗进一步发展了,转基因的 Arabidopsis 幼苗,而是 GUS 活动逐渐地变得弱。GhPIP2; 7 倡导者活动被甘露糖醇处理显著地也导致。而且,酵母房间过去表示的 GhPIP2; 7 显示的相对更高的干旱忍耐,与控制相比。GhPIP2 的在表示上; 7 在提高的 Arabidopsis 种公差到干旱应力。一起,这些数据建议了那 GhPIP2; 7 基因可以涉及叶开发并且响应干旱应力。 | Jie Zhang Dengdi Li Dan Zou Fang Luo Xiulan Wang Yong Zheng Xuebao Li | 2013 | Acta Biochimica et Biophysica Sinica2013，45，2： | 14 |
| 5 | Chinese Medicine Sequential Therapy Improves Pregnancy Outcomes after Surgery for Endometriosis-Associated Infertility: A Multicenter Randomized Double-blind Placebo Parallel Controlled Clinical Trial显示文摘Objective:To evaluate the efficacy and safety of Chinese medicine(CM)improving pregnancy outcomes after surgery for endometriosis-associated infertility.Methods:A multicenter,randomized,doubleblind placebo parallel controlled clinical trial was designed.A total of 202 patients who had laparoscopy for endometriosis-associated infertility with qi stagnation and blood stasis syndrome were included and randomly divided into the CM treatment group and placebo control group at a ratio of 1:1 using a central block randomization from May 2014 to September 2017,101 patients in each group.The two groups received continuous intervention at 1–5 days after surgery,for 6 menstrual cycles.Before ovulation,the CM group was treated Huoxue Xiaoyi Granule(活血消异颗粒);after ovulation,Bushen Zhuyun Granule(补肾助孕颗粒)was involved.The control group was treated with placebo.Transvaginal ultrasonography was performed every menstrual cycle during the treatment,and female hormone levels in the follicular and luteal phases were measured during the 1 st,3 rd and 6 th menstrual cycles.The analysis was continued until pregnancy.The primary outcomes were clinical pregnancy rate and pregnancy outcome,and the secondary outcomes were follicular development and endometrial receptivity.Safety evaluations were performed before and after treatment.Results:(1)Clinical pregnancy and live birth rates:the clinical pregnancy and live birth rates of the CM group were significantly higher than those of the placebo group[44.6%(45/101)vs.29.7%(30/101),34.7%(35/101)vs.20.8%(21/101),both P<0.05].(2)Follicle development:the incidence of dominant follicles,rate of cumulative cycle ovulation,and rate of cumulative cycle mature follicle ovulation were significantly higher in the CM group than those in the placebo group[93.8%(350/373)vs.89.5%(341/381),80.4%(275/342)vs.69.1%(253/366),65.8%(181/275)vs 56.1%(142/253),P<0.05 or P<0.01]).The incidence of cumulative cycle luteinized unruptured follicle syndrome was significantly lower in the CM group than in the placebo group[11.7%(40/342)vs.17.8%(65/366),P<0.05).(3)Endometrial receptivity:after treatment,both endometrial types and endometrial blood flow types in the CM group were mainly types A and B,while those in the placebo group were mainly types B and C,with a significant difference between the two groups(both P<0.05).(4)Adverse events:the incidence of adverse events between the two groups was not significantly different(P>0.05).Conclusion:Strategies for activating blood circulation-regulating Gan(Liver)-tonifying Shen(Kidney)sequential therapy can effectively improve the clinical pregnancy rate and live birth rate of endometriosis-associated infertility with qi stagnation and blood stasis after laparoscopy,improve follicular development,promote ovulation,improve endometrial receptivity,while being a safe treatment option.(Trial registration No.NCT02676713). | ZHAO Rui-hua LIU Yong LU Dan WU Ying WANG Xiao-yun LI Wei-li ZENG Cheng MENG Qing-wei LIAN Feng-mei ZHOU Jun SHI Yun SUN Wei-wei HAN Qian TANG Yi SHI Guang | 2020 | Chinese Journal of Integrative Medicine2020，26，2： | 13 |
| 6 | Emodin alleviates cardiac fibrosis by suppressing activation of cardiac fibroblasts via upregulating metastasis associated protein 3显示文摘Excess activation of cardiac fibroblasts inevitably induces cardiac fibrosis. Emodin has been used as a natural medicine against several chronic diseases. The objective of this study is to determine the effects of emodin on cardiac fibrosis and the underlying molecular mechanisms. Intragastric administration of emodin markedly decreased left ventricular wall thickness in a mouse model of pathological cardiac hypertrophy with excess fibrosis induced by transaortic constriction(TAC) and suppressed activation of cardiac fibroblasts induced by angiotensin II(AngII). Emodin upregulated expression of metastasis associated protein 3(MTA3) and restored the MTA3 expression in the setting of cardiac fibrosis. Moreover, overexpression of MTA3 promoted cardiac fibrosis;in contrast, silence of MTA3 abrogated the inhibitory effect of emodin on fibroblast activation. Our findings unraveled the potential of emodin to alleviate cardiac fibrosis via upregulating MTA3 and highlight the regulatory role of MTA3 in the development of cardiac fibrosis. | Dan Xiao Yue Zhang Rui Wang Yujie Fu Tong Zhou Hongtao Diao Zhixia Wang Yuan Lin Zhange Li Lin Wen Xujuan Kang Philipp Kopylov Dmitri Shchekochikhin Yong Zhang Baofeng Yang | 2019 | Acta Pharmaceutica Sinica B2019，9，4： | 11 |
| 7 | Studies on the overexpression of the soybean GmNHX1 in Lotus corniculatus: The reduced Na^+ level is the basis of the increased salt tolerance显示文摘The full length cDNA coding for a novel vacuolar Na+/H+ antiporter (GmNHX1) was cloned from soybean and determined to consist of 2591 bp with a 5′-untranslated region of 464 bp, an open reading frame (ORF) of 1641 bp, and a 3′-untrans- lated region of 486 bp. The deduced protein se- quence contains 546 aa with the typical characters of the vacuolar Na+/H+ antiporters, and shares high similarity with that of AtNHX1, OsNHX1 and AgNHX1. The soybean genome showed a single copy of the GmNHX1 gene. Northern blot analysis demonstrated that the expression of the GmNHX1 was tissue-spe- cific, increased by ABA treatment, NaCl, KCl, LiCl and dehydration stress, and lower in leaves but higher in roots and hypocotyls of salt-tolerant than salt-sensitive cultivars. The GmNHX1 was overex- pressed under the control of a tandem cauliflower mosaic virus (CaMV) 35S promoter in the model le- guminous plant Lotus corniculatus L. and conferred salt-tolerance of the transgenic plants. Measure- ments of Na+ and K+ contents in both roots and shoots demonstrated that the plantlets of GmNHX1- overexpressing lines had lower Na+ and K+ content, and higher K+/Na+ ratio than the controlled lines that were transformed with the empty vector, which indi- cates that the salt-tolerance conferred by GmNHX1 is closely related with decreased accumulation of Na+ in the transgenic plants. | SUN Yanxiang WANG Dan BAI Yanling WANG Ningning WANG Yong | 2006 | Chinese Science Bulletin2006，51，11： | 10 |
| 8 | Contribution of elF-4E inhibition to the expression and activity of heparanase in human colon adenocarcinoma cell line:LS-174T显示文摘AIM: Heparanase degrades heparan sulfate proteoglycans (HSPGs) and is a critical mediator of tumor metastasis and angiogenesis. Recently, it has been cloned as a single gene family and found to be a potential target for antimetastasis drugs. However, the molecular basis for the regulation of heparanase expression is still not quite clear. The aim of this study was to determine whether the expression of eukaryotic initiation factor 4E (eIF-4E) correlated with the heparanase level in tumor cells and to explore the correlation between heparanase expression and metastatic potential of LS- 174T cells.METHODS: A 20-met antisense s-oligodeoxynucleotide (asODN) targeted against the translation start site of eIF-4E mRNA was introduced into LS-174T cells by lipid-mediated DNA-transfection. eIF-4E protein and mRNA levels were detected by Western blot analysis and RT-PCR, respectively.Heparanase activity was defined as the ability to degrade high molecular weight (40-100 kDa) radiolabeled HS (heparan sulfate) substrate into low molecular weight (5-15 kDa) HS fragments that could be differentiated by gel filtration chromatography. The invasive potential of tumor cell in vitro was observed by using a Matrigel invasion assay system.RESULTS: The 20-mer asODN against eIF-4E specifically and significantly inhibited eIF-4E expression at both transcriptional and translational levels. As a result, the expression and activity of heparanase were effectively retarded and the decreased activity of heparanase resulted in the decreased invasive potential of LS-174T.CONCLUSION: eIF-4E is involved in the regulation of heparanase production in colon adenocarcinoma cell line LS-174T, and its critical function makes it a particularly interesting target for heparanase regulation. This targeting strategy in antisense chemistry may have practical applications in experimental or clinical anti-metastatic gene therapy of human colorectal carcinoma. | Yu-Jie Yang Ya-Li Zhang Xu Li Han-Lei Dan Zhuo-Sheng Lai Ji-De Wang Qun-Ying Wang Hai-Hong Cui Yong Sun Ya-Dong Wang, Chinese PLA Institute of Digestive Disease, Nanfang Hospital, First Military Medical University, Guangzhou 510515, Guangdong Province, China | 2003 | World Journal of Gastroenterology2003，9，8： | 10 |
| 9 | MicroTom Metabolic Network: Rewiring Tomato Metabolic Regulatory Network throughout the Growth Cycle显示文摘Tomato(Solanum lycopersicum)is a major horticultural crop worldwide and has emerged as a preeminent model for metabolic research.Although many research efforts have focused on the analysis of metabolite differences between varieties and species,the dynamics of metabolic changes during the tomato growth cycle and the regulatory networks that underlie these changes are poorly understood.In this study,we integrated high-resolution spatio-temporal metabolome and transcriptome data to systematically explore the metabolic landscape across 20 major tomato tissues and growth stages.In the resulting MicroTom Metabolic Network,the 540 detected metabolites and their co-expressed genes could be divided into 10 distinct clusters based on their biological functions.Using this dataset,we constructed a global map of the major metabolic changes that occur throughout the tomato growth cycle and dissected the underlying regulatory network.In addition to verifying previously well-established regulatory networks for important metabolites,we identified novel transcription factors that regulate the biosynthesis of important secondary metabolites such as steroidal glycoalkaloids and flavonoids.Our findings provide insights into spatiotemporal changes in tomato metabolism and generate a valuable resource for the study of metabolic regulatory processes in model plants. | Yan Li Yang Chen Lu Zhou Shengjie You Heng Deng Ya Chen Saleh Alseekh Yong Yuan Rao Fu Zixin Zhang Dan Su Alisdair R.Fernie Mondher Bouzayen Tao Ma Mingchun Liu Yang Zhang | 2020 | Molecular Plant2020，13，8： | 10 |
| 10 | The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells显示文摘Hepatocyte 生长因素(HGF ) 是导致前列腺癌症细胞增长,移植和侵略的 glycoprotein。短暂受体潜力的激活正规 6 (TRPC6 ) 隧道在支持前列腺癌症房间增长被认为重要。在这研究,我们在前列腺癌症的导致 HGF 的房间增长估计了内长的 TRPC6 隧道的角色。反向的 transcription-PCR 并且西方的弄污被用来调查 TRPC6 表示。Electrophysiological 技术(整个房间的补丁夹钳配置) 并且 Ca2+ 成像分析被用来在房间调查隧道活动。房间周期前进,房间 apoptosis 和房间生长上的 TRPC6 隧道的效果也被检验。TRPC6 并且遇见 c 在 DU145 和 PC3 房间被表示。另外,功能的 TRPC6 隧道在击倒的压制的象 TRPC 一样水流由 oleoyl-2-acetyl-sn-glycerol (OAG ) 唤起了的 DU145 和 PC3 房间,和 TRPC6 是在场的。在 DU145 和 PC3 房间的 TRPC6 隧道的抑制废除了导致 OAG 、导致 HGF 的 Ca2+ 入口。而且, TRPC6 隧道的抑制在 G2/M 阶段逮捕了 DU145 和 PC3 房间并且压制了导致 HGF 的房间增长。一起,我们的结果显示 TRPC6 在导致 HGF 的 DU145 和 PC3 房间增长有一个重要角色。 | Yong Wang Dan Yue Kai Li Yi-Li Liu Chang-Shah Ren Ping Wang | 2010 | Asian Journal of Andrology2010，12，6： | 10 |
| 11 | TRPC6在人良性与恶性前列腺组织中的表达显示文摘观察瞬时受体电位通道C6(TRPC6)在人良性与恶性前列腺组织及前列腺癌细胞系中的表达,进一步探讨TRPC6的表达与前列腺癌分期、分级及激素依赖性的关系。利用免疫组织化学技术,检测发现45.0%的前列腺增生和86.6%的前列腺癌病例表达TRPC6,两者比较有显著性差异沪〈0.01)。TRPC6的表达与前列腺癌分级和前列腺外转移有关(P〈0.01)。前列腺癌分期增高,TRPC6表达增多,但在T2、T3和DT4期肿瘤病例中,TRPC6表达无显著差异。此外TRPC6在激素依赖性前列腺癌与激素非依赖性前列腺癌中的表达也无显著差异。应用RT-PCR及Westernblot,检测到TRPC6在前列腺癌细胞系中的表达。本研究发现,TRPC6在良性与恶性前列腺组织及前列腺癌细胞系中表达。TRPC6的表达与前列腺癌的组织分级、Gleason评分及前列腺外转移有关。 | Dan Yue Yong Wang Jian-Ying Xiaot Ping Wang Chang-Shan Ren | 2009 | Asian Journal of Andrology2009，11，5： | 8 |
| 12 | Self-supported CoMoS4 nanosheet array as an efficient catalyst for hydrogen evolution reaction at neutral pH显示文摘 | Xiang Ren Dan Wu Ruixiang Ge Xu Sun Hongmin Ma Tao Yan Yong Zhang Bin Du Qin Wei Liang Chen | 2018 | Nano Research2018，11，4： | 8 |
| 13 | Regulation of Survivin and CDK4 by Epstein-Barr virus encoded latent mem-brane protein 1 in nasopharyngeal carcinoma cell lines显示文摘Latent membrane protein 1 (LMP1), an important protein encoded by Epstein Barr virus (EBV), has been implied to link with the pathogenesis of nasopharyngeal carcinoma (NPC). Its dual effects of increasing cell proliferation and inhibiting cell apoptosis have been confirmed. In this study, we showed that the expression of Survivin and CDK4 protein in CNE-LMP1, a LMP1 positive NPC epithelial cell line, is higher than in LMP1 negative NPC epithelial cell line- CNE1, and the expression is LMP1 dosage-dependent. Although it was reported that Survivin specifically expressed in cell cycle G2/M phase, our studies suggested that LMP1 could promote the expression of Survivin in G0/G1, S and G2/ M phase. It also showed that Survivin and CDK4 could be accumulated more in the nuclei triggered by LMP1. More interestingly, Survivin and CDK4 could form a protein complex in the nuclei of CNE-LMP1 rather than in that of CNE1, which demonstrated that the interaction between these two proteins could be promoted by LMP1. These results strongly suggested that the role of LMP1 in the regulation of Survivin and CDK4 may also shed some light on the mechanism research of LMP1 in NPC. | Mi Dan AI Li Li LI Xiao Rong ZHAO Yong WU Jian Ping GONG Ya CAO | 2005 | Cell Research2005，15，10： | 8 |
| 14 | Relationship between Maternal PBMC HBV cccDNA and HBV Serological Markers and its Effect on HBV Intrauterine Transmission显示文摘Objective To investigate the relationship between maternal peripheral blood mononuclear cells (PBMC) hepatitis B virus(HBV) covalenty closed circular deoxyribonucleic acid(cccDNA) and other HBV serological markers and its effects on HBV intrauterine transmission. Methods We enrolled 290 newborns and their hepatitis B surface antigen(HBsAg) positive mothers. HBV cccDNA in PBMC and HBV DNA in serum were detected by a real‐time PCR‐TaqM an probe while HBV serological markers were detected with an electrochemiluminescence immunoassay. Results There was a positive correlation between the levels of PBMC HBV cccD NA and serum HBV DNA and HBeA g(r = 0.436 and 0.403, P < 0.001). The detection rate of pattern A [‘HBsA g(+), HBeA g(+), and anti‐HBc(+)’] was significantly higher in the PBMC HBV cccD NA positive group than in the control group(χ^2 = 48.48, P < 0.001). There was a significant association between HBV intrauterine transmission and PBMC HBV cccD NA(χ^2 = 9.28, P = 0.002). In the presence of serum HBV DNA, HBeA g, and PBMC HBV cccD NA, the risk of HBV intrauterine transmission was three times higher(OR = 3.69, 95% CI: 1.30‐10.42) than that observed in their absence. The risk of HBV intrauterine transmission was the greatest(OR = 5.89, 95% CI: 2.35‐14.72) when both PBMC HBV cccD NA and pattern A were present. A Bayesian network model showed that maternal PBMC HBV cccD NA was directly related to HBV intrauterine transmission. Conclusion PBMC HBV cccDNA may be a direct risk factor for HBV intrauterine transmission. Our study suggests that serological markers could be combined with PBMC‐related markers in prenatal testing. | WANG Dan Dan YI Lin Zhu WU Li Na YANG Zhi Qing HAO Hai Yun SHI Xiao Hong WANG Bo FENG Shu Ying FENG Yong Liang WANG Su Ping | 2019 | Biomedical and Environmental Sciences2019，32，5： | 8 |
| 15 | Epigallocatechin gallate inhibits angiotensin II- induced endothelial barrier dysfunction via inhibition of the p38 MAPK/HSP27 pathway显示文摘 | Dan YANG Jing LIU Cui TIAN Yong ZENG Yue-hong ZHENG Quan FANG Hui-hua LI | 2010 | Acta Pharmacologica Sinica2010，31，10： | 8 |
| 16 | The terrestrial NPP simulations in China since Last Glacial Maximum显示文摘Based on Atmosphere-Vegetation Interaction Model (AVIM), the magnitude and spatial distribution of terrestrial net primary productivity (NPP) in China is simu-lated during three different geological eras, Last Glacial Maximum (LGM), Mid-Holocene (MH) and the present. The simulation shows that the glacial-interglacial variation of East Asian summer monsoon in China is the key factor af-fecting the NPP change. During the three eras, mean NPPs are 208 g/m2·a, 409 g/m2·a, and 355 g/m2·a. The total NPPs are 2.05 Pg/a, 3.89 Pg/a and 3.33 Pg/a, respectively. The ter-restrial NPP in China during warm-humid climate is larger than that during cold-arid eras, and the correlation analysis between NPP and climate factors suggests that temperature is the primary factor affecting the terrestrial NPP during 21 kaBP (LGM), and for 6 kaBP (MH) and the present the pri-mary factor is precipitation. | HE Yong DAN Li DONG Wenjie JI Jinjun QIN Dahe | 2005 | Chinese Science Bulletin2005，50，18： | 7 |
| 17 | Connexin 32 and 43 promoter methylation in Helicobacter pylori-associated gastric tumorigenesis显示文摘AIM: To explore the mechanism of abnormal Connexin(Cx) 32 and Cx43 expression in the gastric mucosa after Helicobacter pylori(H. pylori) infection.METHODS: Biopsy specimens of gastric mucosa in different gastric carcinogenesis stages with H. pylori infection, that is, non-atrophic gastritis(NAG; n = 24), chronic atrophic gastritis(CAG; n = 25), intestinal metaplasia(IM; n = 28), dysplasia(DYS; n = 24), andgastric cancer(GC; n = 30), as well as specimens of normal gastric mucosa without H. pylori infection(NGM; n = 25), were confirmed by endoscopy and pathological examination. Cx32 and Cx43 mRNA expression was detected by real-time polymerase chain reaction(PCR). Cx32 and Cx43 promoter CpG island methylation status was determined by methylation-specific PCR(MSP), bisulfite PCR sequencing(BSP) and MassArray methods. RESULTS: The relative mRNA expression levels in the gastric mucosa of patients with NGM, NAG, CAG, IM, DYS and GC were 0.146 ± 0.011, 0.133 ± 0.026, 0.107 ± 0.035, 0.039 ± 0.032, 0.037 ± 0.01 and 0.03 ± 0.011 for Cx32; and 0.667 ± 0.057, 0.644 ± 0.051, 0.624 ± 0.049, 0.555 ± 0.067, 0.536 ± 0.058 and 0.245 ± 0.121 for Cx43, respectively, which were gradually decreasing and significantly different(GC vs NGM: P < 0.001 for Cx32, P < 0.001 for Cx43). The promoter methylation levels in the gastric mucosa from NGM to GC stages by MSP were 38.8% ± 9.0%, 43.1% ± 9.4%, 56.5% ± 3.1%, 64.4% ± 9.7%, 72.5% ± 4.2% and 79.6% ± 6.8% for Cx32; and 49.0% ± 3.9%, 58.1% ± 5.0%, 66.5% ± 7.9%, 74.0% ± 8.8%, 78.3% ± 3.6% and 88.7% ± 6.2% for Cx43, respectively, which were gradually increasing and significantly different(P = 0.039, P = 0.019). The promoter methylation levels by BSP and MassArray exhibited similar trends. Cx32 and Cx43 mRNA expression was negatively correlated with promoter methylation status and gastric carcinogenesis stages(P < 0.001, P = 0.016).CONCLUSION: Cx32 and Cx43 mRNA expression decreased gradually during H. pylori infection-associated gastric carcinogenesis, and it is associated with hypermethylation of these genes' promoter. | Yu Wang Li-Hua Huang Can-Xia Xu Jing Xiao Li Zhou Dan Cao Xiao-Min Liu Yong Qi | 2014 | World Journal of Gastroenterology2014，20，33： | 7 |
| 18 | Spontaneous brain activity in chronic smokers revealed by fractional amplitude of low frequency fluctuation analysis: a resting state functional magnetic resonance imaging study显示文摘 | Chu Shuilian Xiao Dan Wang Shuangkun Peng Peng Xie Teng He Yong Wang Chen | 2014 | Chinese Medical Journal2014，，8： | 6 |
| 19 | Cross-cultural adaption and psychometric properties of the Chinese version of the Diabetes Behavior Rating Scale: a pilot study显示文摘Self-care behavior plays a major role in diabetic management. However, in China, a satisfactory instrument has not yet been developed to evaluate the compliance of self-care behavior for young patients with type 1 diabetes mellitus(T1 DM). The Diabetes Behavior Rating Scale(DBRS) has a potential to be the first mature instrument. The purpose of this study is to cross-culturally adapt the DBRS, and preliminarily evaluate its psychometric properties. The instrument translation included translation, back translation and culture adaptation. Psychometric properties were assessed in a sample of 116 young patients with T1 DM adapting insulin injection therapy. The Chinese version of the DBRS was divided to four subscales. Cronbach's α for the total scale was 0.92. The mean inter-item and item-total correlations were 0.35 and 0.54 respectively. Test-retest reliability showed good temporal stability(r=0.81, P=0.001). Negative correlations were found between DBRS scores with the Diabetes Distress Scale scores(r=.0.32, P=0.003) and hemoglobin A1 c(HbA1 c) levels(r=.0.36, P=0.002). Higher DBRS scores correlated with better glycemic control. The Chinese insulin injection therapy version of the DBRS is well translated and culturally adapted. It shows good overall reliability and validity and appears to be a valuable tool for assessing the diabetic self-care behaviors for young patients with T1 DM. | Jing Zhu Jingjing Xu Yang Chen Yong Gu Li Ji Yufeng Zhou Min Zhu Hsiang-Ting Hsu Xiaoping Huang Cuiping Yuan Yun Shi Dan Yan Lili Xie Shuang Chen Tao Yang Wei He | 2018 | Science China(Life Sciences)2018，61，3： | 6 |
| 20 | Influence of magnetic current on the ground state entanglement in an isotropic transverse XY chain显示文摘We study the ground state entanglement in a three-qubit XY chain under the influence of a magnetization current. It is found that the ground state changes from a W state to a disentangled state as the magnetic field varies. When the Lagrange multiplier λ>3, namely under a strong magnetic current, the ground state holds onto a W state for a large range of the magnetic field strength. A phase transition to the magnetization-current phase occurs under a certain condition. | Liu Dan Zhang Yong Long Guilu | 2007 | Progress in Natural Science:Materials International2007，17，10： | 6 |
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