|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Betulinic acid induces apoptosis of gallbladder cancer cells via repressing SCD1显示文摘Gallbladder cancer(GBC)is the most common and aggressive malignancy of the biliary tract.Betulinic acid(BetA)has been reported to have anti-inflammatory and antitumor effects;however,the effect of BetA on GBC is still unknown.In this study,we investigated the effect of BetA on five GBC cell lines and found that BetA significantly inhibited the proliferation of NOZ cells but had lttle inhibitory effect on other GBC cells.BetA disturbed mitochondrial membrane potential and induced apoptosis in NOZ cells.Real-time polymerase chain reaction analysis revealed that stearoyl-coenzyme A desaturase 1(SCD1)was highly expressed in NOZ cells but low expressed in other GBC cells.BetA inhibited SCD1 expression in a concentration-dependent manner in NOZ cells.Downregulation of SCD1 expression by RNA interference inhibited the proliferation of NOZ cells and induced cell apoptosis.Moreover,BetA inhibited the growth of xenografted tumors and suppressed SCD1 expression in nude mice.Thus,our results showed that BetA induced apoptosis through repressing SCD1 expression in GBC,suggesting that BetA might be an effective agent for the treatment of patients with GBC that highly expresses SCD1. | Hongfei Wang Fangxiao Dong Ye Wang Xu'an Wang Defei Hong Yingbin Liu Jian Zhou | 2020 | Acta Biochimica et Biophysica Sinica2020，52，2： | 6 |
| 2 | Regulation of BMP2K in AP2M1-mediated EGFR internalization during the development of gallbladder cancer显示文摘Dear Editor,Gallbladder cancer(GBC),the most common malignant tumor of the biliary tract,is a highly invasive form of cancer.Surgical resection is currently the first line approach to effectively treat GBC;however,very few patients have the opportunity to receive radical surgical treatment due to lack of obvious symptoms.1 The median survival of patients with GBC is only 12 months and 5-year survival rate is<5%,indicating GBC is extremely poor prognosis.Therefore,it is urgent to identify novel key molecules that can potentially serve as early diagnostic biomarkers and/or therapeutic targets.The current study focused on the potential role of bone morphogenetic protein 2 inducible kinase(BMP2K),a serine/threonine kinase,which was recently identified as clathrin-coated vesicle-associated protein in the development of GBC;the outcome may hold diagnostic and therapeutic promising for clinical practice. | Xiaoling Song Maolan Li Wenguang Wu Wei Dang Yuan Gao Rui Bian Runfa Bao Yunping Hu Defei Hong Jun Gu Yingbin Liu | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 1 |
| 3 | Experimental and molecular simulation studies of CO 2 adsorption on zeolitic imidazolate frameworks: ZIF-8 and amine-modified ZIF-8显示文摘 | Defei Liu Yongbiao Wu Qibin Xia Zhong Li Hongxia Xi | 2013 | Adsorption2013，，1： | 1 |
| 4 | Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories显示文摘High-quality genome-scale metabolic models(GEMs)could play critical roles on rational design of microbial cell factories in the classical Design-Build-Test-Learn cycle of synthetic biology studies.Despite of the constant establishment and update of GEMs for model microorganisms such as Escherichia coli and Saccharomyces cerevisiae,high-quality GEMs for non-model industrial microorganisms are still scarce.Zymomonas mobilis subsp.mobilis ZM4 is a non-model ethanologenic microorganism with many excellent industrial characteristics that has been developing as microbial cell factories for biochemical production.Although five GEMs of Z.mobilis have been constructed,these models are either generating ATP incorrectly,or lacking information of plasmid genes,or not providing standard format file.In this study,a high-quality GEM iZM516 of Z.mobilis ZM4 was constructed.The information from the improved genome annotation,literature,datasets of Biolog Phenotype Microarray studies,and recently updated Gene-Protein-Reaction information was combined for the curation of iZM516.Finally,516 genes,1389 reactions,1437 metabolites,and 3 cell compartments are included in iZM516,which also had the highest MEMOTE score of 91%among all published GEMs of Z.mobilis.Cell growth was then predicted by iZM516,which had 79.4%agreement with the experimental results of the substrate utilization.In addition,the potential endogenous succinate synthesis pathway of Z.mobilis ZM4 was proposed through simulation and analysis using iZM516.Furthermore,metabolic engineering strategies to produce succinate and 1,4-butanediol(1,4-BDO)were designed and then simulated under anaerobic condition using iZM516.The results indicated that 1.68 mol/mol succinate and 1.07 mol/mol 1,4-BDO can be achieved through combinational metabolic engineering strategies,which was comparable to that of the model species E.coli.Our study thus not only established a high-quality GEM iZM516 to help understand and design microbial cell factories for economic biochemical production using Z.mobilis as the chassis,but also provided guidance on building accurate GEMs for other non-model industrial microorganisms. | Yalun Wu Qianqian Yuan Yongfu Yang Defei Liu Shihui Yang Hongwu Ma | 2023 | Synthetic and Systems Biotechnology2023，8，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员