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| 1 | Comparison and Optimization of Multiplexed Quantum Dot-Based Immunohistofluorescence显示文摘Nanoparticle quantum dots (QDs) are ideal materials for multiplexed biomarker detection, localization, and quantification. Both direct and indirect methods are available for QD-based immunohistofluorescence (QD-IHF) staining;the direct method, however, has been considered laborious and costly. In this study, we optimized and compared the indirect QD-IHF single staining procedure using QD-secondary antibody conjugates and QD-streptavidin conjugates. Problems associated with sequential multiplex staining were identified quantitatively. A method using a QD cocktail solution was developed allowing simultaneous staining with three antibodies against E-cadherin, epidermal growth factor receptor and β-catenin in formalin-fixed and paraffin-embedded (FFPE) tissues. The expression of each biomarker was quantified by using the cocktail and the sequential methods. Comparison of the two methods demonstrated that the cocktail method provided more consistent and stable QD signals for each multiplexed biomarker than the sequential method, and provides a convenient tool for multiplexing biomarkers in both research and clinical applications. | Donghai Huang Xianghong Peng Ling Su Dongsheng Wang Fadlo R.Khuri Dong M.Shin Zhuo(Georgia)Chen | 2010 | Nano Research2010，3，1： | 2 |
| 2 | Preparation characterization of LaNi03 A/F ratio-sensitive thin fdm by sol - gel process based on amorphous citrate precursors 显示文摘 | Lu Xuehen Xu Tingxian Dong Xianghong | 2000 | Sensors and Actuators B2000，67，： | 1 |
| 3 | Survival and Metabolic Responses to Freezing Temperature in the Northeast For- est Frog Rana dybowskii 显示文摘 | Xiao Xianghong Zheng Dong Yang Cuijun | 2008 | Asiatic Herpetological Research2008，11，： | 1 |
| 4 | Preparation and characterization of antibacterial viscose-based activated carbon fiber supporting silver显示文摘 | Wang Yulin Wan Yizao Dong Xianghong | 1998 | Carbon1998，36，11： | 1 |
| 5 | Preparation characterization of LaNiO3 A/F ratio-sensitive thin film by sol-gel process based on amorphous citrate precursors显示文摘 | Lu Xuchen Xu Tingxian Dong Xianghong | 2000 | Sensors and Actuators B2000，67，12： | 1 |
| 6 | Effects of Antisense Oligonucleotides against C-Reactive Protein on the Development of Atherosclerosis in WHHL Rabbits显示文摘 | Qi Yu Zhengcao Liu Ahmed Bilal Waqar Bo Ning Xianghong Yang Masashi Shiomi Mark J. Graham Rosanne M. Crooke Enqi Liu Sijun Dong Jianglin Fan Alexander Szalai | 2014 | Mediators of Inflammation2014，，： | 1 |
| 7 | Preparation characterization of LaNiO3 A/F ratio-sensitive thin film by sol-gel process based on amorphous citrate precursors 显示文摘 | Lu Xuchen Xu Tingxian Dong Xianghong | 2000 | Sensors and Actuators B2000，67，： | 1 |
| 8 | Surviva/and metabolic responses to freezing temperature in the northeast forest frog Rana dybowskii 显示文摘 | Xianghong X Dong Z Cuijun Y | 2008 | Asiatic Herpetological Research2008，11，： | 1 |
| 9 | POD Nanozyme optimized by charge separation engineering for light/pH activated bacteria catalytic/photodynamic therapy显示文摘The current feasibility of nanocatalysts in clinical anti-infection therapy,especially for drug-resistant bacteria infection is extremely restrained because of the insufficient reactive oxygen generation.Herein,a novel Ag/Bi2MoO6(Ag/BMO)nanozyme optimized by charge separation engineering with photoactivated sustainable peroxidase-mimicking activities and NIR-II photodynamic performance was synthesized by solvothermal reaction and photoreduction.The Ag/BMO nanozyme held satisfactory bactericidal performance against methicillin-resistant Staphylococcus aureus(MRSA)(~99.9%).The excellent antibacterial performance of Ag/BMO NPs was ascribed to the corporation of peroxidase-like activity,NIR-II photodynamic behavior,and acidity-enhanced release of Ag^(+).As revealed by theoretical calculations,the introduction of Ag to BMO made it easier to separate photo-triggered electronhole pairs for ROS production.And the conduction and valence band potentials of Ag/BMO NPs were favorable for the reduction of O_(2) to·O_(2)^(−).Under 1064 nm laser irradiation,the electron transfer to BMO was beneficial to the reversible change of Mo^(5+)/Mo^(6+),further improving the peroxidase-like catalytic activity and NIR-II photodynamic performance based on the Russell mechanism.In vivo,the Ag/BMO NPs exhibited promising therapeutic effects towards MRSA-infected wounds.This study enriches the nanozyme research and proves that nanozymes can be rationally optimized by charge separation engineering strategy. | Changyu Cao Tingbo Zhang Nan Yang Xianghong Niu Zhaobo Zhou Jinlan Wang Dongliang Yang Peng Chen Liping Zhong Xiaochen Dong Yongxiang Zhao | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 1 |
| 10 | Effects of environmentally relevant concentrations of micro(nano)plastics on aquatic microorganisms:Changes in potential function but not in overall composition显示文摘Micro(nano)plastics(MNPs)are a growing problem as persistent environmental pollutants.Here,we investigated the impact of MNPs on microorganisms in aquatic microbial floc exposed to NPs(80 nm)and MPs(8μm)for 35 days.Water quality indicators were tested weekly and microbiological analyses were conducted on Day 7 and 28 after exposure.The results showed that there were significant differences in the levels of total ammonia nitrogen or nitrite between the MNPs groups and the control group,spanning from Day 7 to Day 28.For the microbial response,microbial community richness in the NPs and MPs groups were significantly increased at Day 7.Functional prediction showed that the relative abundances of bacteria associated with the“Forms Biofilms”,“Potentially Pathogenic”,“Plastic Degradation”and nitrogen cycle processes were significantly different after MNPs exposure.The results suggest that MNPs had no significant effect on the microbial diversity of mature microbial flocs.Findings suggest MPs could cause an increase in the relative abundance of potentially pathogenic bacteria,while NPs do not.In addition,stress associated with MNPs affected the nitrogen cycle of microorganisms,and NPs exerted greater impacts than MPs.Findings from this study further our understanding of the impact of MNPs at environmentally relevant concentrations on microorganisms in aquatic ecosystems. | Zhenlu Wang Xianghong Dong Muzi Zhang Lei Gan Jian Shao Weiling Sun | 2024 | Water Biology and Security2024，3，1： | 0 |
| 11 | Correction To:POD Nanozyme optimized by charge separation engineering for light/pH activated bacteria catalytic/photodynamic therapy显示文摘Correction to:Signal Transduction and Targeted Therapy http://gffzzd3cc09b8251d45dfsuqck9c9pcwpq6bfw.ffgz.tsg.suse.edu.cn/10.1038/s41392-022-00900-8,published online 28 March 2022 The plate photo from Control in Figure 4c of the published work was mistakenly used when editing the photos,and the corrected version is demonstrated below.The results and conclusions of this paper were not affected by this error. | Changyu Cao Tingbo Zhang Nan Yang Xianghong Niu Zhaobo Zhou Jinlan Wang Dongliang Yang Peng Chen Liping Zhong Xiaochen Dong Yongxiang Zhao | 2023 | Signal Transduction and Targeted Therapy2023，8，6： | 0 |
| 12 | Countermeasures for Constructing Provincial Modern Agricultural Industrial Park:Taking Jellyfish Industry,the Leading Industry of Yingkou City as an Example显示文摘At present,modern agricultural industrial park with large-scale planting and breeding base as a platform has become an innovative measure to promote the development of regional modern agriculture and an important starting point for the implementation of rural industry revitalization,and it has been highly valued by local governments.Yingkou City has established and developed a modern agricultural industrial park with jellyfish as the leading industry.This will effectively fill the gap in this work in Yingkou City and boost the development of the jellyfish industry.This study provides strong support for the promotion of the city card of'China's Hometown of Jellyfish'. | Qiuxiang HAN Shengzhong DONG Ting LIU Xianghong LIU | 2020 | Asian Agricultural Research2020，12，8： | 0 |
| 13 | Synergetic treatment of oxygen microcapsules and lenvatinib for enhanced therapy of HCC by alleviating hypoxia condition and activating anti-tumor immunity显示文摘Hypoxia is a typical characteristic of hepatocellular carcinoma(HCC), which causes tremendous obstacles to tumor treatments. Current first-line treatment may further deteriorate tumor hypoxia. For example,Lenvatinib, a receptor tyrosine kinase inhibitor(RTKI), suppresses tumor growth via blocking vascular endothelial growth factor(VEGF) signaling, and can also inhibit angiogenesis, thus limiting oxygen supply to tumor sites. Therefore, alleviating tumor microenvironment(TME) hypoxia holds great potential for enhancing the therapeutic effect of RTKI. Here, nanoparticle-stabilized oxygen microcapsules, a stable and biocompatible oxygen-loaded delivery system, are successfully prepared through interfacial polymerization of polydopamine nanoparticles. The microcapsules with a large loading capacity of oxygen in the core show excellent bioavailability and dispersity, which could effectively improve the hypoxic TME when they serve as oxygen delivery vehicles. Synergetic treatments of Lenvatinib and oxygen microcapsules could induce the transition of “cold tumor” in an immune-suppressed state to “hot tumor” in an immune-activated state by improving tumor hypoxic TME and reducing angiogenesis in HCC. It is revealed that combined treatments of oxygen microcapsules and Lenvatinib could polarize tumor-associated macrophages(TAMs) to anti-tumor M1 cells and activate T cell-mediated anti-tumor immune responses.The results suggest that synergetic therapy using oxygen microcapsules and Lenvatinib could alleviate the hypoxic TME and enhance the therapeutic performance of RTKI, demonstrating a promising anti-tumor strategy for enhanced therapy of HCC. | Jianpeng Sheng Jiangchao Wu Xianghong Yin Zhu Sun Xun Wang Junlei Zhang Jianghui Tang Yongtao Ji Jinyuan Song Xiaobao Wei Lin Wang Yaxing Zhao Hui Zhang Taohong Li Qi Zhang Xueli Bai Li Chen Dong Chen Tingbo Liang | 2023 | Chinese Chemical Letters2023，34，4： | 0 |
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