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| 1 | CRISPR-Cas9-mediated genome editing in one blastomere of two-cell embryos reveals a novel Tet3 function in regulating neocortical development显示文摘学习必要基因的早功能是在发展生物学的一个重要、挑战性的问题。这里,我们为很快在二房间的阶段胚胎的一个分裂球导致 CRISPR-Cas9-mediated 变化建立了一个方法,称为的 2 房间 embryo-CRISPR-Cas9 注射(2CC ) ,学习在里面必需品的 vivo 功能(或未知) 在创始人的基因妄想的鼠标。由注射指向兴趣的基因进的 Cre mRNA 和 CRISPR-Cas9 荧光灯记者老鼠, 2CC 方法能在不同发展阶段跟踪野类型、变异的房间,为变异的房间的 phenotypic 分析的出现内部控制。用这个方法,我们在在发展中的鼠标调整有刺激性、禁止的 synaptic 传播识别了必要基因 Tet3 的新奇函数服的外皮。由产生妄想的变异的老鼠, 2CC 方法允许在多重纸巾和房间的基因功能的快速的屏蔽录入妄想的老鼠创始人,显著地扩展基因工具的当前的一套设备。 | Wang, Lingbo Li, Min-Yin Qu, Chao Miao, Wan-Ying Yin, Qi Liao, Jiaoyang Cao, Hua-Teng Huang, Min Wang, Kai Zuo, Erwei Peng, Guangdun Zhang, Shu-Xin Chen, Guodong Li, Qing Tang, Ke Yu, Qian Li, Zhoujie Wong, Catherine C. L. Xu, Guoliang Jing, Naihe Yu, Xiang Li, Jinsong | 2017 | Cell Research2017，27，6： | 5 |
| 2 | Recent advances in wireless epicortical and intracortical neuronal recording systems显示文摘An implantable brain-computer interface(BCI)has proven to be effective in the field of sensory and motor function restoration and in the treatment of neurological disorders.Using a BCI recording system,we can transform current methods of human interaction with machines and the environment,especially to help those with cognitive and mobility disabilities regain mobility and reintegrate into society.However,most reported work has focused on a simple aspect of the whole system,such as electrodes,circuits,or data transmission,and only a very small percentage of systems are wireless.A miniature,lightweight,wireless,implantable microsystem is key to realizing long-term,real-time,and stable monitoring on freely moving animals or humans in their natural conditions.Here,we summarize typical wireless recording systems,from recording electrodes,processing chips and controllers,wireless data transmission,and power supply to the system-level package for either epicortical electrocorticogram(ECoG)or intracortical local field potentials(LFPs)/spike acquisition,developed in recent years.Finally,we conclude with our vision of challenges in next-generation wireless neuronal recording systems for chronic and safe applications. | Bowen JI Zekai LIANG Xichen YUAN Honglai XU Minghao WANG Erwei YIN Zhejun GUO Longchun WANG Yuhao ZHOU Huicheng FENG Honglong CHANG Jingquan LIU | 2022 | Science China(Information Sciences)2022，65，4： | 2 |
| 3 | Growth Habit of Rutile and a-Alz O3 Crystals显示文摘 | Li Wenjun Shi Erwei Yin Zhiwen | 2000 | Journal of Crystal Growth2000，208，13： | 1 |
| 4 | A novel task-oriented optimal design for P300 - based brain-computer interfaees显示文摘 | Zhou Zongtan Yin Erwei Liu Yang | 2014 | J NeuralEng2014，11，05： | 1 |
| 5 | Human 8-cell embryos enable efficient induction of disease-preventive mutations without off-target effect by cytosine base editor显示文摘Approximately 140 million people worldwide are homozygous carriers of APOE4(ε4),a strong genetic risk factor for late onset familial and sporadic Alzheimer’s disease(AD),91%of whom will develop AD at earlier age than heterozygous carriers and noncarriers.Susceptibility to AD could be reduced by targeted editing of APOE4,but a technical basis for controlling the off-target effects of base editors is necessary to develop low-risk personalized gene therapies.Here,we first screened eight cytosine base editor variants at four injection stages(from 1-to 8-cell stage),and found that FNLS-YE1 variant in 8-cell embryos achieved the comparable base conversion rate(up to 100%)with the lowest bystander effects.In particular,80%of AD-susceptibleε4 allele copies were converted to the AD-neutralε3 allele in humanε4-carrying embryos.Stringent control measures combined with targeted deep sequencing,whole genome sequencing,and RNA sequencing showed no DNA or RNA off-target events in FNLS-YE1-treated human embryos or their derived stem cells.Furthermore,base editing with FNLS-YE1 showed no effects on embryo development to the blastocyst stage.Finally,we also demonstrated FNLS-YE1 could introduce known protective variants in human embryos to potentially reduce human susceptivity to systemic lupus erythematosus and familial hypercholesterolemia.Our study therefore suggests that base editing with FNLS-YE1 can efficiently and safely introduce known preventive variants in 8-cell human embryos,a potential approach for reducing human susceptibility to AD or other genetic diseases. | Yinghui Wei Meiling Zhang Jing Hu Yingsi Zhou Mingxing Xue Jianhang Yin Yuanhua Liu Hu Feng Ling Zhou Zhifang Li Dongshuang Wang Zhiguo Zhang Yin Zhou Hongbin Liu Ning Yao Erwei Zuo Jiazhi Hu Yanzhi Du Wen Li Chunlong Xu Hui Yang | 2023 | Protein & Cell2023，14，6： | 0 |
| 6 | Electromyography signal segmentation method based on spectral subtraction backtracking显示文摘Surface electromyography(EMG) is a bioelectrical signal that recognizes speech contents in a non-acoustic form. Activity detection is an important research direction in EMG research. However, in the low signal-to-noise ratio(SNR) environment, it is difficult for traditional methods to obtain accurate active signals. This paper proposes a new energy-based spectral subtraction backtracking(E-SSB) method to segment EMG active signal in the low SNR environment. Compared with traditional energy detection, the algorithm in this paper adds spectral subtraction(SS) to filter out the clutter, and raises a retrospective idea to improve the classification performance. The experiment results show the proposed activity detection method is more effective than other methods in the low SNR environment. | CAI Huihui ZHANG Yakun XIE Liang YIN Erwei YAN Ye MING Dong | 2022 | Optoelectronics Letters2022，18，10： | 0 |
| 7 | Growth habit of polar crystals显示文摘Using coordination polyhedron rule, growth habit of polar crystals such as ZnO, ZnS and SiO2 is investigated. It shows that the growth rates in the positive and negative polar axis directions are different. The theoretical growth habit of ZnO crystal is hexagonal prism and the growth rates of its various faces are: V{0001} >V{0111} > V{0110} > V{0111} > V{0001}. The growth habit of ZnScrystal is tetrahedron and its growth rates of different crystal faces are: V(111}>V{001}>V(001}=V{100} = V{010}>V{111}. The growth rate relationship between positive and negative polar axis directionsof SiO2 crystal is V{1120} > V{1120}. These results are in agreement with the growth habits observedunder hydrothermal conditions. The different growth rates between positive and negative polar axis directions cannot be explained by PBC theory. | Wenjun Li Erwei Shi Zhizhan Chen Zhiwen Yin | 2000 | Chinese Science Bulletin2000，45，18： | 0 |
| 8 | New Ion Thruster Testing Succeeded显示文摘The 200+mm ion thruster engineering prototype developed by the Lanzhou Institute of Physics passed its testing,with the main performance specifications such as thrust and specific impulse meeting the requirements of users.Based on the overall structure and manufacturing processes of the 200mm ion thruster,the 200+mm Ion | YIN Changhong ZHANG Erwei | 2015 | Aerospace China2015，16，4： | 0 |
| 9 | Quantitative characterization of filamentous fungal promoters on a single-cell resolution to discover cryptic natural products显示文摘Characterization of filamentous fungal regulatory elements remains challenging because of time-consuming transformation technologies and limited quantitative methods.Here we established a method for quantitative assessment of filamentous fungal promoters based on flow cytometry detection of the superfolder green fluorescent protein at single-cell resolution.Using this quantitative method,we acquired a library of 93 native promoter elements from Aspergillus nidulans in a high-throughput format.The strengths of identified promoters covered a 37-fold range by flow cytometry.P_(zipA) and P_(sltA)were identified as the strongest promoters,which were 2.9-and 1.5-fold higher than that of the commonly used constitutive promoter P_(gpdA).Thus,we applied P_(zipA)and P_(sltA)to activate the silent nonribosomal peptide synthetase gene Afpes1 from Aspergillus fumigatus in its native host and the heterologous host A.nidulans.The metabolic products of Afpes1 were identified as new cyclic tetrapeptide derivatives,namely,fumiganins A and B.Our method provides an innovative strategy for natural product discovery in fungi. | Peng-Lin Wei Jie Fan Jingwen Yu Zihui Ma Xian Guo Nancy PKeller Erwei Li Chunbo Lou Wen-Bing Yin | 2023 | Science China(Life Sciences)2023，66，4： | 0 |
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