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| 1 | SHP2 promotes proliferation of breast cancer cells through regulating Cyclin D1 stabilityviathe PI3K/AKT/GSK3β signaling pathway显示文摘Objective:The tyrosine phosphatase SHP2 has a dual role in cancer initiation and progression in a tissue type-dependent manner.Several studies have linked SHP2 to the aggressive behavior of breast cancer cells and poorer outcomes in people with cancer.Nevertheless,the mechanistic details of how SHP2 promotes breast cancer progression remain largely undefined.Methods:The relationship between SHP2 expression and the prognosis of patients with breast cancer was investigated by using the TCGA and GEO databases.The expression of SHP2 in breast cancer tissues was analyzed by immunohistochemistry.CRISPR/Cas9 technology was used to generate SHP2-knockout breast cancer cells.Cell-counting kit-8,colony formation,cell cycle,and EdU incorporation assays,as well as a tumor xenograft model were used to examine the function of SHP2 in breast cancer proliferation.Quantitative RT-PCR,western blotting,immunofluorescence staining,and ubiquitination assays were used to explore the molecular mechanism through which SHP2 regulates breast cancer proliferation.Results:High SHP2 expression is correlated with poor prognosis in patients with breast cancer.SHP2 is required for the proliferation of breast cancer cellsin vitro and tumor growthin vivo through regulation of Cyclin D1 abundance,thereby accelerating cell cycle progression.Notably,SHP2 modulates the ubiquitin–proteasome-dependent degradation of Cyclin D1viathe PI3K/AKT/GSK3βsignaling pathway.SHP2 knockout attenuates the activation of PI3K/AKT signaling and causes the dephosphorylation and resultant activation of GSK3β.GSK3βthen mediates phosphorylation of Cyclin D1 at threonine 286,thereby promoting the translocation of Cyclin D1 from the nucleus to the cytoplasm and facilitating Cyclin D1 degradation through the ubiquitin–proteasome system.Conclusions:Our study uncovered the mechanism through which SHP2 regulates breast cancer proliferation.SHP2 may therefore potentially serve as a therapeutic target for breast cancer. | Yue Yuan Yanling Fan Zicong Gao Xuan Sun He Zhang Zhiyong Wang Yanfen Cui Weijie Song Zhaosong Wang Fei Zhang Ruifang Niu | 2020 | Cancer Biology & Medicine2020，17，3： | 11 |
| 2 | The heterogeneity of islet autoantibodies and the progression of islet failure in type 1 diabetic patients显示文摘Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus. | Jin Liu Lingling Bian Li Ji Yang Chen Heng Chen Yong Gu Bingqin Ma Wei Gu Xinyu Xu Yun Shi Jian Wang Dalong Zhu Zilin Sun Jianhua Ma Hui Jin Xing Shi Heng Miao Bing Xin Yan Zhu Zhenwen Zhang Ruifang Bu Lan Xu Guangde Shi Wei Tang Wei Li Dongmei Zhou Jun Liang Xingbo Cheng Bimin Shi Jixiang Dong Ji Hu Chen Fang Shao Zhong Weinan Yu Weiping Lu Chenguang Wu Li Qian Jiancheng Yu Jialin Gao Xiaoqiang Fei Qingqing Zhang Xueqin Wang Shiwei Cui Jinluo Cheng Ning Xu Guofeng Wang Guoqing Han Chunrong Xu Yun Xie Minmin An Wei Zhang Zhixiao Wang Yun Cai Qi Fu Yu Fu Shuai Zheng Fan Yang Qingfang Hu Hao Dai Yu Jin Zheng Zhang Kuanfeng Xu Yifan Li Jie Shen Hongwen Zhou Wei He Xuqin Zheng Xiao Han Liping Yu Jinxiong She Mei Zhang Tao Yang | 2016 | Science China(Life Sciences)2016，59，9： | 5 |
| 3 | Effects of demineralization on phenols distribution and formation during coal pyrolysis显示文摘 | Yonghui Bai Lunjing Yan Guanlong Li Ruifang Zhao Fan Li | 2014 | Fuel2014，，： | 2 |
| 4 | Variation trends and influencing factors of total gaseous mercury in the Pearl River Delta—A highly industrialised region in South China influenced by seasonal monsoons显示文摘 | Laiguo Chen Ming Liu Zhencheng Xu Ruifang Fan Jun Tao Duohong Chen Deqiang Zhang Donghai Xie Jiaren Sun | 2013 | Atmospheric Environment2013，，： | 1 |
| 5 | Detection of telomerase activity in biopsy samples for predicting prognosis in cirrhotic patients with hepatocellular carcinoma after laparoscopic radiofrequency ablation therapy显示文摘Objective: To explore the role of telomerase activity detected in biopsy samples for evaluating the efficacy of lapa- roscopic radiofrequency ablation (RFA) therapy in patients with hepatocellular carcinoma (HCC) and liver cirrhosis. Methods: From August 2001 to October 2004, 34 cirrhotic patients with HCC were treated by laparoscopic RFA under general anesthe- sia. A total of 34 tumors, with a mean maximum tumor diameter of 4.0 ± 1.0 cm, were all located on the liver surface or adja- cent to the gallbladder. Laparoscopic ultrasound-guided core biopsy for liver lesions was performed before and immediately after RFA therapy. In these biopsy samples, telomerase activity was detected by the ELISA-based telomeric repeat amplifica- tion protocol (ELISA-TRAP) assay, and pathological examination was routinely performed. Results: Laparoscopic RFA was successfully performed in all the 34 patients. A complete tumor necrosis was achieved in all patients on the contrast-enhanced helical CT scanning one month after laparoscopic RFA. The positive rates of telomerase activity and histopathologic diagnosis in biopsy samples were 91.2% (31/34) and 100% (34/34) respectively before RFA, and 26.5% (9/34) and 0% respectively after RFA. During a median follow-up period of 35 months (range, 18–51 months), the rates of local tumor recurrence at the ablation sites in post-RFA telomerase-positive and negative patients were 88.9% (8/9) and 4% (1/25) respectively (P < 0.01), and the rates of distant recurrence within the livers were 0% (0/9) and 12% (3/25) respectively (P > 0.05). Conclusion: For cirrhotic patients with HCC treated by laparoscopic RFA, detection of telomerase activity in biopsy samples may be useful for evaluating the therapeutic efficacy of RFA and predicting postoperative local tumor recurrence. | Ruifang Fan Fulu Chai Zhipeng Han Chenyang Wang Xianling Guo Xinxin Bu Lixin Wei | 2007 | The Chinese-German Journal of Clinical Oncology2007，6，3： | 1 |
| 6 | Improving aromatic hydrocarbons yield from coal pyrolysis volatile products over HZSM-5 and Mo-modified HZSM-5显示文摘 | Li Guanlong Yan Lunjing Zhao Ruifang Li Fan | 2014 | Fuel2014，130，15： | 1 |
| 7 | Determination of ten monohydroxylated polycyclic aromatic hydrocarbons by liquid-liquid extraction and liquid chromatography/tandem mass spectrometry显示文摘 | Ruifang Fan Robert Ramage Dongli Wang | 2012 | Talanta2012，93，： | 1 |
| 8 | Down regu lation of telomcrase activity and activation of caspase-3 areresponsible for tanshinone I induced apoptosis in monocyte leukemia cells in z,itro 显示文摘 | Liu XiaoDan Fan RuiFang Zhang Yong et aZ | 2010 | Int J Mol Sci2010，11，6： | 1 |
| 9 | Dopamine receptor D 1 mediates the inhibition of dopamine on the distal colonic motility显示文摘 | Xiaohui Zhang Hua Guo Jindong Xu Yun Li Lisheng Li Xiaoli Zhang Xiaofeng Li Ruifang Fan Yue Zhang Zhongping Duan Jinxia Zhu | 2012 | Translational Research2012，，5： | 1 |
| 10 | Radio-frequency-assisted Liver Partition With Portal Vein Ligation (RALPP) for Liver Regeneration显示文摘 | Tamara M. H. Gall Mikael H. Sodergren Adam E. Frampton Ruifang Fan Duncan R. Spalding Nagy A. Habib Madhava Pai James E. Jackson Paul Tait Long R. Jiao | 2015 | Annals of Surgery2015，，： | 1 |
| 11 | 减毒沙门氏菌介导的NK4基因对HepG2细胞致癌作用的影响(英文)显示文摘Objective:The aim of the study was to construct a stable strain of recombined attenuated Salmonella typhimurium expressing NK4 gene,and observe the effect of the strain on the metastatic potentiality of HepG2 cells.Methods:The NK4 cDNA was isolated from PCAGGS/hNK4 plasmid by PCR,and subcloned into eukaryotic expression vector pcDNA4.The recombinant plasmid was electro-transferred into attenuated Salmonella typhimurium Ty21a to obtain the recombinant strain encoding NK4 gene(TPN).Simultaneously,the recombinant attenuated Salmonella typhimurium carrying GFP gene(TPG) was also constructed.After the TPG and TPN were transferred into HepG2 cells,the transfection rate and the expression level of NK4 protein were detected by flow cytometry and ELISA,and the effects of expression product on the proliferation and migration of HepG2 and angiogenesis were observed.Results:The TPN and TPG were successfully constructed.Fortyeight hours after transfection with TPG,the infection rate was 82.58% ± 1.74%,and the expression level of NK4 protein in supernatant was(181.5 ± 11.7) ng/6 × 105 cells.The supernatant had obviously depressant effect on the proliferative activity of HepG2 cells(P < 0.05),and could obviously restrain the hepatocyte growth factor-mediated migration of tumor cells(P < 0.01).The inhibitory effect of the expression product on the tumor angiopoiesis was obviously observed(P < 0.05),without a dosage-effect relation.Conclusion:The TPN could effectively transfer tumor cells in vitro and express interest NK4 protein.The expression product could effectively inhibit the proliferation and migration of hepatocellular carcinoma cells and the tumor angiopoiesis. | Xiaoqin Ha Ruifang Fan Tongde Lv Yuebin Xu Ling Hui Xiaopeng Yang Qinhua Jia | 2009 | The Chinese-German Journal of Clinical Oncology2009，8，12： | 0 |
| 12 | Adjuvant chemotherapy versus adjuvant concurrent chemoradiotherapy after radical surgery for early-stage cervical cancer:a randomized,non-inferiority,multicenter trial显示文摘We conducted a prospective study to assess the non-inferiority of adjuvant chemotherapy alone versus adjuvant concurrent chemoradiotherapy (CCRT) as an alternative strategy for patients with early-stage (FIGO 2009 stage IB–IIA) cervical cancer having risk factors after surgery. The condition was assessed in terms of prognosis, adverse effects, and quality of life. This randomized trial involved nine centers across China. Eligible patients were randomized to receive adjuvant chemotherapy or CCRT after surgery. The primary end-point was progression-free survival (PFS). From December 2012 to December 2014, 337 patients were subjected to randomization. Final analysis included 329 patients, including 165 in the adjuvant chemotherapy group and 164 in the adjuvant CCRT group. The median follow-up was 72.1 months. The three-year PFS rates were both 91.9%, and the five-year OS was 90.6% versus 90.0% in adjuvant chemotherapy and CCRT groups, respectively. No significant differences were observed in the PFS or OS between groups. The adjusted HR for PFS was 0.854 (95% confidence interval 0.415–1.757;P = 0.667) favoring adjuvant chemotherapy, excluding the predefined non-inferiority boundary of 1.9. The chemotherapy group showed a tendency toward good quality of life. In comparison with post-operative adjuvant CCRT, adjuvant chemotherapy treatment showed non-inferior efficacy in patients with early-stage cervical cancer having pathological risk factors. Adjuvant chemotherapy alone is a favorable alternative post-operative treatment. | Danhui Weng Huihua Xiong Changkun Zhu Xiaoyun Wan Yaxia Chen Xinyu Wang Youzhong Zhang Jie Jiang Xi Zhang Qinglei Gao Gang Chen Hui Xing Changyu Wang Kezhen Li Yaheng Chen Yuyan Mao Dongxiao Hu Zimin Pan Qingqin Chen Baoxia Cui Kun Song Cunjian Yi Guangcai Peng Xiaobing Han Ruifang An Liangsheng Fan Wei Wang Tingchuan Xiong Yile Chen Zhenzi Tang Lin Li Xingsheng Yang Xiaodong Cheng Weiguo Lu Hui Wang Beihua Kong Xing Xie Ding Ma | 2023 | Frontiers of Medicine2023，17，1： | 0 |
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