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| 1 | Variation in cis-regulation of a NAC transcription factor contributes to drought tolerance in wheat显示文摘Drought is a major environmental factor limiting wheat production worldwide,and developing drought-tolerant cultivars is a central challenge for wheat breeders globally.Therefore,it is important to identify genetic components determining drought tolerance in wheat.In this study,we identified a wheat NAC gene(TaNAC071-A)that is tightly associated with drought tolerance by a genome-wide association study.Knockdown of TaNAC071-A in wheat attenuated plant drought tolerance,whereas its overexpression significantly enhanced drought tolerance through improved water-use efficiency and increased expression of stress-responsive genes.This heightened water-saving mechanism mitigated the yield loss caused by water deficit.Further candidate gene association analysis showed that a 108-bp insertion in the promoter of TaNAC071-A alters its expression level and contributes to variation in drought tolerance among wheat accessions.This insertion contains two MYB cis-regulatory elements(CREs)that can be directly bound by the MYB transcription activator,TaMYBL1,thereby leading to increased TaNAC071-A expression and plant drought tolerance.Importantly,introgression of this 108-bp insertion allele,TaNAC071-AIn-693,into drought-sensitive cultivars could improve their drought tolerance,demonstrating that it is a valuable genetic resource for wheat breeding.Taken together,our findings highlight a major breakthrough in determining the genetic basis underlying phenotypic variation in wheat drought tolerance and showcase the potential of exploiting CRE-containing indels for improving important agronomical traits. | Hude Mao Shumin Li Bin Chen Chao Jian Fangming Mei Yifang Zhang Fangfang Li Nan Chen Tian Li Linying Du Li Ding Zhongxue Wang Xinxiu Cheng Xiaojing Wang Zhensheng Kang | 2022 | Molecular Plant2022，15，2： | 9 |
| 2 | Aberrant translation regulated by METTL1/WDR4-mediated tRNA N7-methylguanosine modification drives head and neck squamous cell carcinoma progression显示文摘Background:Cancer cells selectively promote the translation of oncogenic tran-scripts to stimulate cancer progression.Although growing evidence has revealed that tRNA modifications and related genes participate in this process,their roles in head and neck squamous cell carcinoma(HNSCC)remain largely unchar-acterized.Here,we sought to investigate the function and mechanisms of the transfer RNA(tRNA)N7-methylguanosine(m'G)modification in regulating the occurrence and development of HNSCC.Methods:Cell lost of-function and gain-of function assays,xenograft models,conditional knockout and knockin mouse models were used to study the physi-ological functions of tRNA m'G modification in HNSCC tumorigenesis.tRNA modification and expression profiling,mRNA translation profiling and res-cue assays were performed to uncover the underlying molecular mechanisms.Single-cell RNA sequencing(scRNA seq)was conducted to explore the tumor microenvironment changes.Results:The tRNA.m7G methyltransferase complex components Methyltransferase-like 1(METTL1)/WD repeat domain 4(WDR4)were upregulated in HNSCC and associated with a poor prognosis.Functionally,METTL1/WDR4 promoted HNSCC progression and metastasis in cell-based and transgenic mouse models.Mechanistically,ablation of METTL1 reduced the m'G levels of 16 tRNAS,inhibiting the translation of a subset of oncogenic transcripts,including genes related to the phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/AKT/mTOR)signaling pathway.In addition,chemical modulators of the PI3K/Akt/mTOR signaling pathway reversed the effects of Mettll in mouse HNSCC.Furthermore,scRNA-seq results revealed that Mettll knockout in mouse tumor cells altered the immune landscape and cell-cell interaction between the tumor and stromal compartment.Conclusions:The tRNA m?G methyltransferase METTLI was found to promote the development and malignancy of HNSCC through regulating global mRNA translation,including the PI3K/AKT/mTOR signaling pathway,and found to alter immune landscape.METTLI could be a promising treatment target for HNSCC patients. | Jie Chen Kang Li Jianwen Chen Xiaochen Wang Rongsong Ling Maosheng Cheng Zhi Chen Fangfang Chen Qianting He Shuai Li Caihua Zhang Yizhou Jiang Qianming Chen Anxun Wang Demeng Chen | 2022 | Cancer Communications2022，42，3： | 6 |
| 3 | Methyltransferase like 13 mediates the translation of Snail in head and neck squamous cell carcinoma显示文摘Methyltransferase like 13(METTL13),a kind of methyltransferase,is implicated in protein binding and synthesis.The upregulation of METTL13 has been reported in a variety of tumors.However,little was known about its potential function in head and neck squamous cell carcinoma(HNSCC)so far.In this study,we found that METTL13 was significantly upregulated in HNSCC at both mRNA and protein level.Increased METTL13 was negatively associated with clinical prognosis.And METTL13 markedly affected HNSCC cellular phenotypes in vivo and vitro.Further mechanism study revealed that METTL13 could regulate EMT signaling pathway by mediating enhancing translation efficiency of Snail,the key transcription factor in EMT,hence regulating the progression of EMT.Furthermore,Snail was verified to mediate METTL13-induced HNSCC cell malignant phenotypes.Altogether,our study had revealed the oncogenic role of METTL13 in HNSCC,and provided a potential therapeutic strategy. | Xiaochen Wang Kang Li Yuehan Wan Fangfang Chen Maosheng Cheng Gan Xiong Ganping Wang Shuang Chen Zhi Chen Jianwen Chen Xiuyun Xu Cheng Wang Liang Peng Demeng Chen | 2021 | International Journal of Oral Science2021，13，3： | 3 |
| 4 | The interactions between cGAS-STING pathway and pathogens显示文摘Cytosolic DNA is an indicator of pathogen invasion or DNA damage.The cytosolic DNA sensor cyclic guanosine monophosphateadenosine monophosphate(cGAMP)synthase(cGAS)detects DNA and then mediates downstream immune responses through the molecule stimulator of interferon genes(STING,also known as MITA,MPYS,ERIS and TMEM173).Recent studies focusing on the roles of the cGAS-STING pathway in evolutionary distant species have partly sketched how the mammalian cGAS-STING pathways are shaped and have revealed its evolutionarily conserved mechanism in combating pathogens.Both this pathway and pathogens have developed sophisticated strategies to counteract each other for their survival.Here,we summarise current knowledge on the interactions between the cGAS-STING pathway and pathogens from both evolutionary and mechanistic perspectives.Deeper insight into these interactions might enable us to clarify the pathogenesis of certain infectious diseases and better harness the cGAS-STING pathway for antimicrobial methods. | Zhangliang Cheng Tong Dai Xuelin He Zhengkui Zhang Feng Xie Shuai Wang Long Zhang Fangfang Zhou | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 3 |
| 5 | The role of a drug-loaded poly(lactic co-glycolic acid)(PLGA) copolymer stent in the treatment of ovarian cancer显示文摘Objectives:Cisplatin(CDDP)is a widely used and effective basic chemotherapeutic drug for the treatment of a variety of tumors,including ovarian cancer.However,adverse side effects and acquired drug resistance are observed in the clinical application of CDDP.Identifying a mode of administration that can alleviate side effects and reduce drug resistance has become a promising strategy to solve this problem.Methods:In this study,3 D printing technology was used to prepare a CDDP-poly(lactic-co-glycolic acid)(CDDP-PLGA)polymer compound stent,and its physicochemical properties and cytotoxicity were evaluated both in vitro and in vivo.Results:The CDDP-PLGA stent had a significant effect on cell proliferation and apoptosis and clearly decreased the size of subcutaneous tumors in nude mice,whereas the systemic side effects were mild compared with those of intraperitoneal CDDP injection.Compared with the control group,CDDP-PLGA significantly increased the mRNA and protein levels of p-glycoprotein(P<0.01;P<0.01)and decreased vascular endothelial growth factor mRNA(P<0.05)and protein levels(P<0.01),however,CDDP-PLGA significantly decreased the mR NA and protein levels of p-glycoprotein(P<0.01;P<0.01)and vascular endothelial growth factor(P<0.01;P<0.01),which are associated with chemoresistance,in subcutaneous tumor tissue.Immunohistochemistry assay results revealed that,in the CDDP-PLGA group,the staining of the proliferation-related genes Ki67 and PCNA were lightly,and the apoptosis-related gene caspase-3 stained deeply.Conclusions:PLGA biomaterials loaded with CDDP,as compared with the same amount of free CDDP,showed good efficacy in terms of cytotoxicity,as evidenced by changes in apoptosis.Continuous local CDDP release can decrease the systemic side effects of this drug and the occurrence of drug resistance and angiogenesis,and improve the therapeutic effect.This new approach may be an effective strategy for the local treatment of epithelial ovarian cancer. | Yanqing Wang Xiaoyin Qiao Xiao Yang Mengqin Yuan Shu Xian Li Zhang Dongyong Yang Shiyi Liu Fangfang Dai Zhikai Tan Yanxiang Cheng | 2020 | Cancer Biology & Medicine2020，17，1： | 2 |
| 6 | The Scorpion Venom Peptide Smp76 Inhibits Viral Infection by Regulating Type-Ⅰ Interferon Response显示文摘Dengue virus(DENV) and Zika virus(ZIKV) have spread throughout many countries in the developing world and infect millions of people every year, causing severe harm to human health and the economy. Unfortunately, there are few effective vaccines and therapies available against these viruses. Therefore, the discovery of new antiviral agents is critical.Herein, a scorpion venom peptide(Smp76) characterized from Scorpio maurus palmatus was successfully expressed and purified in Escherichia coli BL21(DE3). The recombinant Smp76(rSmp76) was found to effectively inhibit DENV and ZIKV infections in a dose-dependent manner in both cultured cell lines and primary mouse macrophages. Interestingly,rSmp76 did not inactivate the viral particles directly but suppressed the established viral infection, similar to the effect of interferon(IFN)-b. Mechanistically, rSmp76 was revealed to upregulate the expression of IFN-b by activating interferon regulatory transcription factor 3(IRF3) phosphorylation, enhancing the type-Ⅰ IFN response and inhibiting viral infection.This mechanism is significantly different from traditional virucidal antimicrobial peptides(AMPs). Overall, the scorpion venom peptide Smp76 is a potential new antiviral agent with a unique mechanism involving type-Ⅰ IFN responses,demonstrating that natural AMPs can enhance immunity by functioning as immunomodulators. | Zhenglin Ji Fangfang Li Zhiqiang Xia Xingchen Guo Minjun Gao Fang Sun Yuting Cheng Yingliang Wu Wenxin Li Syed Abid Ali Zhijian Cao | 2018 | Virologica Sinica2018，33，6： | 2 |
| 7 | LILRB4 ITIMs mediate the T cell suppression and infiltration of acute myeloid leukemia cells显示文摘We recently demonstrated that leukocyte Ig-like receptor 4(LILRB4)expressed by monocytic acute myeloid leukemia(AML)cells mediates T-cell inhibition and leukemia cell infiltration via its intracellular domain.The cytoplasmic domain of LILRB4 contains three immunoreceptor tyrosine-based inhibitory motifs(ITIMs);the tyrosines at positions 360,412,and 442 are phosphorylation sites.Here,we analyzed how the ITIMs of LILRB4 in AML cells mediate its function.Our in vitro and in vivo data show that Y412 and Y442,but not Y360,of LILRB4 are required for T-cell inhibition,and all three ITIMs are needed for leukemia cell infiltration.We constructed chimeric proteins containing the extracellular domain of LILRB4 and the intracellular domain of LILRB1 and vice versa.The intracellular domain of LILRB4,but not that of LILRB1,mediates T-cell suppression and AML cell migration.Our studies thus defined the unique signaling roles of LILRB4 ITIMs in AML cells. | Zunling Li Mi Deng Fangfang Huang Changzhu Jin Shuang Sun Heyu Chen Xiaoye Liu Licai He Ali HSadek Cheng Cheng Zhang | 2020 | Cellular & Molecular Immunology2020，17，3： | 1 |
| 8 | The diagnostic criteria for primary osteoporosis and the incidence of osteoporosis in China显示文摘 | Zhonghou Liu Junhong Piao Lianping Pang Xiang Qing Su Nan Zi’ang Pan Yichao Guo Xiaohong Wang Fangfang Li Jingping Liu Xiaoguang Cheng | 2002 | Journal of Bone and Mineral Metabolism2002，，4： | 1 |
| 9 | The diagnostic criteria for primary osteoporosis and the incidence of osteoporosis in China显示文摘 | Zhonghou Liu Junhong Piao Lianping Pang Xiang Qing Su Nan Zi’ang Pan Yichao Guo Xiaohong Wang Fangfang Li Jingping Liu Xiaoguang Cheng | 2002 | Journal of Bone and Mineral Metabolism2002，，4： | 1 |
| 10 | Micelle dyeing with low liquor ratio for reactive dyes using dialkyl maleic acid es- ter surfactants 显示文摘 | Xie Kongliang Cheng Fangfang | 2011 | Journal of Cleaner Production2011，19，： | 1 |
| 11 | Protective effect of areca inflorescence extract on hydrogen peroxide-in- duced oxidative damage to human serum albumin显示文摘 | Fangfang Cheng Weijun Chen Yulin Huang | 2011 | Food Re- search International2011，44，1： | 1 |
| 12 | Desorption behaviors of BDE-28 and BDE-47 from natural soils with dif- ferent organic carbon contents显示文摘 | LIU Wenxin CHENG Fangfang LI Weibo | 2012 | Environmental Pollution2012，163，4： | 1 |
| 13 | An integrative methodology to improve brownfield redevelopment planning in Chinese cities:A case study of Fution,Shenzhen显示文摘 | Fangfang Cheng Stan Geertman Monika Kuffer | | 0，，05： | 1 |
| 14 | Magnesium ion-implantation-based gallium nitride p-i-n photodiode for visible-blind ultraviolet detection显示文摘In this work, a GaN p-i-n diode based on Mg ion implantation for visible-blind UV detection is demonstrated.With an optimized implantation and annealing process, a p-GaN layer and corresponding GaN p-i-n photodiode are achieved via Mg implantation. As revealed in the UV detection characterizations, these diodes exhibit a sharp wavelength cutoff at 365 nm, high UV/visible rejection ratio of 1.2 × 10~4, and high photoresponsivity of 0.35 A/W, and are proved to be comparable with commercially available GaN p-n photodiodes. Additionally, a localized states-related gain mechanism is systematically investigated, and a relevant physics model of electricfield-assisted photocarrier hopping is proposed. The demonstrated Mg ion-implantation-based approach is believed to be an applicable and CMOS-process-compatible technology for GaN-based p-i-n photodiodes. | WEIZONG XU YATING SHI FANGFANG REN DONG ZHOU LINLIN SU QING LIU LIANG CHENG JIANDONG YE DUNJUN CHEN RONG ZHANG YOUDOU ZHENG HAI LU | 2019 | Photonics Research2019，7，8： | 1 |
| 15 | Protective effect of areca inflorescence extract on hydrogen peroxide-induced oxidative damage to human serum albumin显示文摘 | Fangfang Cheng Weijun Chen Yulin Huang | 2011 | Food Research International2011，44，1： | 1 |
| 16 | Purification and enzymatic characterization of alcohol dehydrogenase from Arabidopsis thaliana显示文摘 | CHENG Fangfang TAO Hu YAN An | 2013 | Protein Expression and Purification2013，90，2： | 1 |
| 17 | ADT-OH improves intestinal barrier function and remodels the gut microbiota in DSS-induced colitis显示文摘Owing to the increasing incidence and prevalence of inflammatory bowel disease(IBD)worldwide,effective and safe treatments for IBD are urgently needed.Hydrogen sulfide(H2S)is an endogenous gasotransmitter and plays an important role in inflammation.To date,H2S-releasing agents are viewed as potential anti-inflammatory drugs.The slow-releasing H_(2)S donor 5-(4-hydroxyphenyl)-3H-1,2-dithiole-3-thione(ADT-OH),known as a potent therapeutic with chemopreventive and cytoprotective properties,has received attention recently.Here,we reported its anti-inflammatory effects on dextran sodium sulfate(DSS)-induced acute(7 days)and chronic(30 days)colitis.We found that ADT-OH effectively reduced the DSS-colitis clinical score and reversed the inflammation-induced shortening of colon length.Moreover,ADT-OH reduced intestinal inflammation by suppressing the nuclear factor kappa-B pathway.In vivo and in vitro results showed that ADT-OH decreased intestinal permeability by increasing the expression of zonula occludens-1 and occludin and blocking increases in myosin II regulatory light chain phosphorylation and epithelial myosin light chain kinase protein expression levels.In addition,ADT-OH restored intestinal microbiota dysbiosis characterized by the significantly increased abundance of Muribaculaceae and Alistipes and markedly decreased abundance of Helicobacter,Mucispirillum,Parasutterella,and Desulfovibrio.Transplanting ADT-OH-modulated microbiota can alleviate DSS-induced colitis and negatively regulate the expression of local and systemic proinflammatory cytokines.Collectively,ADT-OH is safe without any short-term(5 days)or long-term(30 days)toxicological adverse effects and can be used as an alternative therapeutic agent for IBD treatment. | Zhiqian Bi Jia Chen Xiaoyao Chang Dangran Li Yingying Yao Fangfang Cai Huangru Xu Jian Cheng Zichun Hua Hongqin Zhuang | 2023 | Frontiers of Medicine2023，17，5： | 0 |
| 18 | Correction to: LILRB4 ITIMs mediate the T cell suppression and infiltration of acute myeloid leukemia cells显示文摘In this article,published online on 7 November 2019,there was an unintended error during image processing of Fig.1a and Fig.6c leading to wrong pictures to appear.These pictures have now been replaced with the appropriate pictures that were generated for this experiment.The corrected Fig.1 and Fig.6 are shown here.The conclusion made from the experiments remains unaltered.The inconvenience caused by this error is deeply regretted. | Zunling Li Mi Deng Fangfang Huang Changzhu Jin Shuang Sun Heyu Chen Xiaoye Liu Licai He Ali H.Sadek Cheng Cheng Zhang | 2020 | Cellular & Molecular Immunology2020，17，3： | 0 |
| 19 | Experimental investigation on weak shock wave mitigation characteristics of flexible polyurethane foam and polyurea显示文摘In recent years,explosion shock wave has been considered as a signature injury of the current military conflicts.Although strong shock wave is lethal to the human body,weak shock wave can cause many more lasting consequences.To investigate the protection ability and characteristics of flexible materials and structures under weak shock wave loading,the blast wave produced by TNT explosive is loaded on the polyurethane foam with the density of 200.0 kg/m3(F-200)and 400.0 kg/m3(F-400),polyurea with the density of 1100.0 kg/m^(3)(P-1100)and structures composed of the two materials,which are intended for individual protection.Experimental results indicate that the shock wave is attenuated to weak pressure disturbance after interacting with the flexible materials which are not damaged.The shock wave protective capability of single-layer materials is dependent on their thickness,density and microscopic characteristics.The overpressure,maximum pressure rise rate and impulse of transmitted wave decrease exponentially with increase in sample thickness.For the same thickness,F-400 provides better protective capability than F-200 while P-1100 shows the best protective capability among the three materials.In this study,as the materials are not destroyed,F-200 with a thickness more than10.0 mm,F-400 with a thickness more than 4.0 mm,and P-1100 with a thickness more than 1.0 mm can attenuate the overpressure amplitude more than 90.0%.Further,multi-layer flexible composites are designed.Different layer layouts of designed structures and layer thickness of the single-layer materials can affect the protective performance.Within the research range,the structure in which polyurea is placed on the impact side shows the optimal shock wave protective performance,and the thicknesses of polyurea and polyurethane foam are 1.0 mm and 4.0 mm respectively.The overpressure attenuation rate reached maximum value of 93.3%and impulse attenuation capacity of this structure are better than those of single-layer polyurea and polyurethane foam with higher areal density. | Shiyu Jia Cheng Wang Wenlong Xu Dong Ma Fangfang Qi | 2024 | Defence Technology(防务技术)2024，31，1： | 0 |
| 20 | Phase transition of SrCo_(0.9)Fe_(0.1)O_(3) electrocatalysts and their effects on oxygen evolution reaction显示文摘Complex metal oxide has emerged as an important class of electrocatalyst for oxygen evolution reaction(OER)because of its flexibility in the crystal phase and relative ease in the control of defect structures.While different crystalline phases and solid structures may be produced from the same reactant precursors,their phase transitions and OER performances are not well understood.In this work,we present the preparation of different phases of SrCo_(0.9)Fe_(0.1)O_(3-δ)(nominal ratio)solids using a sol-gel method and the structure-catalytic property relationship of both crystalline and amorphous structures formed at different temperatures.We observed as synthetic temperature increased,phase and composition transitions occurred from the amorphous phase to strontium carbonate and cobalt oxide,to oxygen-deficient perovskite,and finally to perovskite.The OER activity depended highly on crystal structures.The oxygen-deficient perovskite prepared at 750℃ exhibited the highest catalytic activity,while the perovskite showed the lowest activity.X-ray photoelectron spectroscopy analysis reveals the change of oxidation state occurred during the phase transition.This work provides a useful guideline for the design of OER electrocatalysts through controlling the crystal structures and defects in complex metal oxides,such as perovskite. | Cheng Zhang Fangfang Wang Mariam Batool Beichen Xiong Hong Yang | 2022 | SusMat2022，2，4： | 0 |
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