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| 1 | mTOR/miR-145-regulated exosomal GOLM1 promotes hepatocellular carcinoma through augmented GSK-3b/MMPs显示文摘Golgi membrane protein 1(GOLM1/GP73)is a serum marker of hepatocellular carcinoma(HCC).We have previously shown that mTOR promoted tumorigenesis of HCC through stimulating GOLM1 expression.In this study,we demonstrated that the mammalian target of rapamycin(mTOR)was a negative regulator of microRNA-145(miR-145)expression.miR-145 inhibited GOLM1 expression by targeting a coding sequence of GOLM1 gene.GOLM1 and miR-145 were inversely correlated in human HCC tissues.GOLM1-enriched exosomes activated the glycogen synthase kinase-3β/matrix metalloproteinases(GSK-3β/MMPs)signaling axis of recipient cells and accelerated cell proliferation and migration.In contrast,miR-145 suppressed tumorigenesis and metastasis.We suggest that mTOR/miR-145/GOLM1 signaling pathway should be targeted for HCC treatment. | Xiaochen Gai Bufu Tang Fangming Liu Yuting Wu Fang Wang Yanling Jing Fuqiang Huang Di Jin Ling Wang Hongbing Zhang | 2019 | Journal of Genetics and Genomics2019，46，5： | 13 |
| 2 | Extraction, identification, and antioxidant property evaluation of limonin from pummelo seeds显示文摘Limonin, the main bioactive phytochemical constituent of limonoids with multi-functions, is enriched in citrus fruits and often found at a high concentration in citrus seeds. The present study was attempted to introduce a new and efficient extraction method to isolate limonoids from pummelo seeds, and to evaluate the antioxidant property of the main constituent limonin in Hep G2 cells. Three key single factors were identified for the extraction of limonoids from pummelo seeds using the Box-Behnken experiment design of response surface methodology(RSM), and the optimized extraction parameters were treatment with 89.68 m L of anhydrous acetone for 4.62 h at 78.94C, while the yield of limonoids was 11.52 mg/g. The structure of isolated main constituent of the limonoids was further identified as limonin by Fourier transform infrared(FT-IR) spectrometer and nuclear magnetic resonance(NMR)spectrum. Moreover, the molecular data in Hep G2 cells revealed that limonin exerted its anti-oxidant property mainly by the activation of nuclear factor(erythroid-2)-like 2(Nrf2)/kelch-like ECHassociated protein 1(Keap1)-antioxidant response element(ARE) pathway in the form of transcriptional regulation of Nrf2 m RNA and posttranscriptional regulation of Nrf2/Keap1 system. These results demonstrate that pummelo seeds are an ideal source of limonoids, and limonin is proved to exert its anti-oxidant property by the activation of Nrf2/Keap1 pathway. | Si Qin Chenghao Lv Qingshan Wang Zhibing Zheng Xi Sun Minyi Tang Fangming Deng | 2018 | Animal Nutrition2018，，3： | 10 |
| 3 | Free cholesterol-induced macrophage apoptosis is mediated by inositol-requiring enzyme 1 alpha-regulated activation of Jun N-terminal kinase显示文摘在先进动脉粥样硬化患者损害的巨噬细胞死亡导致 lesional 坏死,可能的匾破裂,和尖锐脉管的吸藏。巨噬细胞死亡的一个可能的原因是导致 endoplasmic 蜂窝胃的激活的免费胆固醇(FC ) 的累积(嗯) 导致压力的 apoptosis。1 alpha (IRE1 ) 是的要求肌醇的酶 ERthat 的不可分的膜蛋白质是在在巨噬细胞的导致胆固醇的 apoptosis 的关键发信号步,在 ER 由应力激活。然而,在规定的 IRE1 的角色嗯为这个过程的导致压力的巨噬细胞死亡和机制大部分是不清楚的。在这研究,一个房间文化模型被用来探索涉及导致 FC 的巨噬细胞死亡的 ER 压力小径的机制。结果此处证明巨噬细胞装载的 FC 导致部分依赖于由 IRE1 的激活的开始的 apoptotic 回答。一起拿,这些结果证明 IRE1-apoptosis-signaling kinase 1-c-Jun NH2 终端 kinase 串联小径在这个过程被要求。而且,数据在先进动脉粥样硬化患者损害为导致胆固醇的巨噬细胞死亡建议了新奇细胞的机制。这发信号的串联的批评功能被预防显示嗯在 IRE1 的抑制以后的导致压力的 apoptosis,或 c6 月 NH2 终端 kinase。 | Fangming Li Yi Guo Shenggang Sun Xin Jiang Bingshan Tang Qizhang Wang Ling Wang | 2008 | Acta Biochimica et Biophysica Sinica2008，40，3： | 7 |
| 4 | Polyacrylic acid sodium salt film entrapped Ag-nanocubes as molecule traps for SERS detection显示文摘提高表面的拉曼光谱学(重量的单位) 是为踪迹化学药品的一种快分析技术;然而,吸附 analytes 要求活跃重量的单位底层,因此为底层与需要的亲密关系限制目标种类到那些。这里我们现在的联网的 polyacrylic 酸钠盐(PAAS ) 电影骗诱 Ag-nanocubes (作为 Ag-nanocubes@PAAS 表示了) 作为为有或没有高亲密关系的 analytes 的有效重量的单位底层。一次 analyte 水的答案在干燥 Ag-nanocubes@PAAS 底层上被扔,嗜酒的 PAAS 变得肿强迫 Ag-nanocubes 松开,当时在在 Ag-nanocubes 之中的空隙弥漫的 analytes。当弄干时, PAAS 缩小并且拉 Ag-nanocubes 回到他们的以前的聚集的状态,当 PAAS 网络为重量的单位察觉在在 Ag-nanocubes 之间的小差距耽搁 analytes 时。没有强壮的亲密关系,策略为 Ag 为不仅 singleanalytes 而且 multi-analytes 被证明有效,在环境在能吸附、非能吸附的污染物质的基于重量的单位的同时的 multi-analyte 察觉显示出它的潜力。 | Zhulin Huang Guowen Meng Qing Huang Bin Chen Fei Zhou Xiaoye Hu Yiwu Qian Haibin Tang Fangming Han Zhaoqin Chu | 2014 | Nano Research2014，7，8： | 6 |
| 5 | Prevalence, risk factors, and survival associated with pulmonary hypertension and heart failure among patients with underlying coronary artery disease: a national prospective, multicenter registry study in China显示文摘Background: Coronary artery disease (CAD) is the commonest cause of heart failure (HF), whereas pulmonary hypertension (PH) has not been established or reported in this patient population. Therefore, we assessed the prevalence, risk factors, and survival in CAD-associated HF (CAD-HF) complicated with PH.Methods: Symptomatic CAD-HF patients were continuously enrolled in this prospective, multicenter registry study. Echocardiography, coronary arteriography, left and right heart catheterization (RHC), and other baseline clinical data were recorded. Patients were followed up and their survival was recorded.Results: One hundred and eighty-two CAD-HF patients were enrolled, including 142 with HF with a preserved ejection fraction (heart failure with preserved ejection fraction [HFpEF];left ventricular ejection fraction [LVEF] ≥50%) and 40 with a reduced ejection fraction (heart failure with reduced ejection fraction [HFrEF];LVEF < 50%). PH was diagnosed with RHC in 77.5% of patients. Patients with PH showed worse hemodynamic parameters and higher mortality. HFrEF-PH patients had worse survival than HFpEF-PH patients. CAD-HF patients with an enlarged left ventricular end-diastolic diameter and reduced hemoglobin were at higher risk of PH. Nitrate treatment reduced the risk of PH. Elevated creatinine and mean pulmonary arterial pressure (mPAP), diastolic pressure gradient (DPG) ≥7 mmHg, and previous myocardial infarction (MI) entailed a higher risk of mortality in CAD-HF patients with PH.Conclusions: PH is common in CAD-HF and worsens the hemodynamics and survival in these patients. Left ventricle enlargement and anemia increase the risk of PH in CAD-HF. Patients may benefit from nitrate medications. Renal impairment, elevated mPAP, DPG ≥7 mmHg, and previous MI are strong predictors of mortality in CAD-HF-PH patients.Trial Registration: ClinicalTrials.gov, NCT02164526. | Li Huang Lingpin Pang Qing Gu Tao Yang Wen Li Ruilin Quan Weiqing Su Weifeng Wu Fangming Tang Xiulong Zhu Jieyan Shen Jingzhi Sun Guangliang Shan Changming Xiong Shian Huang Jianguo He | 2022 | Chinese Medical Journal2022，，15： | 4 |
| 6 | Dynamic changes in fibrinogen levels of patients with acute cerebral infarction after taking defibrase显示文摘BACKGROUND: At present, as a therapeutic drug mainly for reducing fibrinogen (FIB) levels, the dynamic influence of defibrase on the FIB levels of patients with acute cerebral infarction has not been clearly ascertained. OBJECTIVE: To observe the dynamic changes in FIB levels of patients with acute cerebral infarction at different time points after taking defibrase. DESIGN, TIME AND SETTING: Randomized controlled clinical trial. The study was conducted in the Department of Neurology, the Second Affiliated Hospital of Jinan University, from June to November 2006. PARTICIPANTS: Sixty patients with acute cerebral infarction, who had been treated by the Neurological Department of the Second Affiliated Hospital of Jinan University from June to November 2006, were selected, including 37 males and 23 females, aged 35–75 years. All cases met the diagnostic criteria formulated by the Fourth National Cerebrovascular Disease Conference within 12 hours of onset. All the patients were confirmed with definite hemiparesis and cerebral infarction without coma, and were randomly divided into two groups: a treatment group (n =40) and a control group (n =20). Patients’ families had the right to be informed and agree with the treatment, which had permission from the Hospital Ethics Committee. METHODS: Patients in the control group were given routine treatment with 30 mL fleabane and 0.75 g cytidine diphosphate added to 500 mL saline solution once a day for 14 consecutive days. Patients in the treatment group were given routine treatment and Haiwang defibrase injection (purchased from Changchu Guoao Bio-Pharmaceutical Co. Ltd., Approval document number H10983237) within 12 hours of infarction. Defibrase doses of 15, 12.5 and 10 U were given over 2 hours according to the patients’ pre-treatment plasma FIB levels of ≥ 4.50 g/L, 3.50-4.49 g/L and 1.00-3.49 g/L, respectively. Plasma FIB levels in the treatment group were measured before, and once every six hours for 48 hours after administration of defibrase. Later, measurements were taken once every 12 hours and FIB levels were kept in a range of 0.5–1.3 g/L for one week. When the FIB level increased to over 1.3 g/L, a 5 U dose of defibrase was given again over two hours. FIB levels of the control group were measured once before treatment and once after one week of treatment MAIN OUTCOME MEASURES: (1) Dynamic changes of FIB levels in the defibrase treatment group. (2) Comparison of dynamic changes of FIB levels in the treatment group and control group before and after treatment for one week. RESULTS: All 60 patients were included in the final analysis. The treatment group’s FIB levels quickly decreased to 0.5–1.3 g/L within 12 hours of taking the first dose of defibrase and reached a minimum in 24 hours. Later, they began to rise slowly into the therapeutic range (0.5–1.3 g/L) in 48 hours. The FIB levels of the treatment group increased slowly to over 1.3 g/L in 60 hours after the first dose of defibrase and then quickly decreased to the therapeutic range after the second dose of 5 U defibrase with a minimum level higher than after the first dose, and higher again after the third dose. After treatment, patients’ FIB levels could be kept in the therapeutic range for about one week. The FIB levels in patients in the treatment group were significantly lower after taking defibrase for one week in comparison with the levels before treatment (P < 0.01). There was no difference in the control group between the levels pre-treatment and one week after treatment (P > 0.05). CONCLUSIONS: FIB levels in patients with acute cerebral infarction quickly decrease after taking the first dose of defibrase and reach a minimum in 24 hours. Later, they begin to rise slowly into the therapeutic range (0.5–1.3 g/L) in 48 hours. The FIB levels increase slowly to over 1.3 g/L in 60 hours. The effect of defibrase is weaker when the same dose of defibrase is used in patients repeatedly. | Yi Guo Ling Wang Bingshan Tang Fangming Li Qizhang Wang | 2008 | Neural Regeneration Research2008，3，5： | 3 |
| 7 | Metal sulfide quantum dots-aggregated PAMAM dendrimer for cadmium ion-selective electrode-based immunoassay of alpha-fetoprotein显示文摘An ion-selective electrode(ISE)-based immunoassay has been innovatively designed for the sensitive detection of liver cancer biomarker(alpha-fetoprotein,AFP),using metal sulfide quantum dot(QD)-based nano labels.Cd S QDs-aggregated PAMAM dendrimer(QD-DE)was first synthesized and functionalized with polyclonal rabbit anti-human AFP antibodies.Thereafter,a sandwich immunoreaction was implemented on monoclonal mouse anti-human AFP antibody-coated microplate by using antibody-functionalized QD-DE as the secondary antibody.Accompanying the immunocomplex,subsequent potentiometric detection of cadmium ion dissolved from the QD-DE under acidic condition was conducted on a portable cadmium ion-selective electrode(Cd-ISE).Results revealed that the electrode potential of the Cd-ISE increased with the increment of AFP concentration from 0.1 to 100 ng m L^(-1)at a detection limit(LOD)of 68 pg m L^(-1).The relative standard deviations(RSD)were below9.09%and 10.54%for the intra-and inter-assay,respectively.Additionally,six human serum specimens were determined on CdISE-based immunosensor by using commercial human AFP ELISA kit as the reference,and gave good relationship between two methods.Importantly,Cd-ISE-based immunoassay offers the promise for simple and cost-effective screening of disease-related biomarkers. | Qunfang Li Jing Jin Fangming Lou Dianping Tang | 2018 | Science China Chemistry2018，61，6： | 2 |
| 8 | JNK Suppresses Apoptosis via Phosphorylation of the Proapoptotic Bcl-2 Family Protein BAD显示文摘 | Chenfei Yu Yuzuru Minemoto Jiyan Zhang Jing Liu Fangming Tang Truc N. Bui Jialing Xiang Anning Lin | 2004 | Molecular Cell2004，，3： | 1 |
| 9 | Study on human myosin MLCK kinase显示文摘 | TANG FANGMING WANG RAN GENG DEQING | 1995 | Actaof Xuzhou Medical College1995，15，4： | 1 |
| 10 | Structure and electrochemical properties of hypo-stoichiometric hydro-gen storage alloys prepared by twin-roller rapid quenching process显示文摘The stoichiometric alloy MlB5.0 and the hypo-stoichiometric alloy MlB4.85 were prepared by twin-roller rapid quenching process, and their structure and electrochemical properties were studied. The results of XRD show that both of the alloys have a typical single-phase hexagonal CaCu5-type structure. The cell volume of the hypo-stoichiometric alloy MlB4.85 is slightly larger than that of the stoichiometric alloy MlB5.0, although its lattice constant c/a is smaller. Under 2 C discharging rate, i.e. 640 mA/g, the MlB4.85 has a discharge capacity of 320 mAh/g, which is higher than that of the MlB5.0, 312 mAh/g. Nevertheless, the capacities of the MlB4.85 and the MlB5.0 decline 24.7% and 20.2% after 400 cycles, respec- tively. The relationship of electrochemical performances of the alloys with their structures is discussed. | ZHOU Liu LU Dongsheng LI Weishan HU Shejun CAI Yuepeng TANG Renheng XIAO Fangming | 2005 | Rare Metals2005，24，4： | 1 |
| 11 | Modeling of a transmission line protection relaying scheme using Petri nets显示文摘 | Wang Fangming Tang Jianxin | 1997 | IEEE Trans on Power Delivery1997，12，3： | 1 |
| 12 | Lead, zinc and cadmium accumulation in herbaceous species and soils in Lanping Pb/Zn mining area,Yunnan Province, China显示文摘 | Yetao TANG Rongliang QIU Xiaowen ZENG Xiaohang FANG Xiaoyong ZHOU Fangming YU Yudu WU | 2006 | Chinese Journal Of Geochemistry2006，25，B08： | 1 |
| 13 | Modeling of a transmission line protection relaying scheme using Petri nets显示文摘 | WANG Fangming TANG Jianxin | 1997 | IEEE Trans on Power Delivery1997，12，3： | 1 |
| 14 | SARS-CoV-2-specific immune response in COVID-19 convalescent individuals显示文摘We collected blood from coronavirus disease 2019(COVID-19)convalescent individuals and investigated SARS-CoV-2-specific humoral and cellular immunity in these discharged patients.Follow-up analysis in a cohort of 171 patients at 4-11 months after the onset revealed high levels of IgG antibodies.A total of 78.1%(164/210)of the specimens tested positive for neutralizing antibody(NAb).SARS-CoV-2 antigen peptide pools-stimulated-IL-2 and-IFN-y response can distinguish COVID-19 convalescent individuals from healthy donors.Interestingly,NAb survival was significantly affected by the antigen peptide pools-stimulated-IL-2 response,-IL-8 response,and-IFN-y response.The antigen peptide pools-activated CD8+T cell counts were correlated with NAb.The antigen peptide pools-activated natural killer(NK)cell counts in convalescent individuals were correlated with NAb and disease severity.Our data suggested that the development of NAb is associated with the activation of T cells and NK cells.Our work provides a basis for further analysis of the protective immunity to SARS-CoV-2 and for understanding the pathogenesis of COVID-19.It also has implications for the development of an effective vaccine for SARS-CoV-2 infection. | Yunbao Pan Xianghu Jiang Liu Yang Liangjun Chen Xiaojiao Zeng Guohong Liu Yueting Tang Chungen Qian Xinming Wang Fangming Cheng Jun Lin Xinghuan Wang Yirong Li | 2021 | Signal Transduction and Targeted Therapy2021，6，8： | 0 |
| 15 | Correction:SARS-CoV-2-specific immune response in COYID-19 convalescent individuals显示文摘After online publication of the article1 the authors noticed an error in the figure legends of Fig.1 and Supplementary Materials Fig.S2,where the'*P<0.05,0.05<**P<0.001,***P<0.001W.should be replaced by n*P<0.05,**P<0.01,***P<0.001M.The original article has been corrected. | Yunbao Pan Xianghu Jiang Liu Yang Liangjun Chen Xiaojiao Zeng Guohong Liu Yueting Tang Chungen Qian Xinming Wang Fangming Cheng Jun Lin Xinghuan Wang Yirong Li | 2021 | Signal Transduction and Targeted Therapy2021，6，8： | 0 |
| 16 | Characteristic Investigation of Nano-Crystal Hydrogen Storage Alloys显示文摘A Ml (NiCoMnA1)5 hydrogen storage alloys was prepared by double-roller rapid quenching.Its microstructure, electrochemically and kinetic characteristic were studied.A uniform crystal phase with CaCu5 structure could be detected by XRD analyses, whose average grain size is 30 ~ 50 nm and the ratio of c/a of nano-crystal hydrogen storage alloy is larger.The hydrogen absorption/desertion p - C isotherms of alloy show that its fiat-performance is perfect and the magnetic stagnant effect is very little.An simulate cell is used for electrochemical measurement.Electrode is 10C, the capacity decreasing rate via the 450 cycles at 7C is less than 20%. | Xiao Fangming Huang Lili Tang Renheng Lu Qiyun Peng Neng Wang Ying | 2004 | Journal of Rare Earths2004，22，z1： | 0 |
| 17 | Human Capital Development and Its Influence on FDI Withdrawal:An Empirical Analysis显示文摘This paper examines the causal effect of human capital expansion on the exit of foreignowned firms using the difference-in-differences method together with a quasi-natural experiment involving the expansion of higher education in China in 1999.The expansion of human capital reduces the probability of the exit of foreign-owned firms significantly This conclusion remains robust under a series of tests,including changing the sample range,adjusting the identification method,and considering the trade liberalization.Analysis of the mechanism indicates that human capital expansion reduces the exit probability of foreign-owned firms by stimulating innovation,improving production efficiency,and optimizing the quality of input and output goods.Industries with higher asset specificity are also more likely to restrain the exit of foreign-owned firms through human capital expansion.Overall,the findings of this study provide a good insight into the exit behavior of FDI in China from the perspective of human capital. | Chuanchuan Li Lei Li Haodan Tang Yanyan Zheng Fangming Gong | 2023 | China & World Economy2023，31，5： | 0 |
| 18 | Overview of Digital Image Restoration显示文摘Image restoration is an image processing technology with great practical value in the field of computer vision.It is a computer technology that estimates the image information of the damaged area according to the residual image information of the damaged image and carries out automatic repair.This article firstly classify and summarize image restoration algorithms,and describe recent advances in the research respectively from three aspects including image restoration based on partial differential equation,based on the texture of image restoration and based on deep learning,then make the brief analysis of digital image restoration of subjective and objective evaluation method,and briefly summarize application of digital image restoration technique in the future and prospects,provide direction for the research on image after repair. | Wei Chen Tingzhu Sun Fangming Bi Tongfeng Sun Chaogang Tang Biruk Assefa | 2019 | Journal of New Media2019，1，1： | 0 |
| 19 | THE CRACK-INCLUSION INTERACTION AND THE ANALYSIS OF SINGULARITY FOR THE HORIZONTAL CONTACT显示文摘Using the basic solutions of a single crack and a single inclusion, and making use of the principle of linear superposition of elastic mechanics, the interaction problem between a planar crack and a flat inclusion in an elastic solid is studied. The problem is reduced to solve a set of standard Cauchy_type singular equations. And the stress intensity factors at points of crack and inclusion were obtained. Besides, the singularity for the horizontal contact of crack and inclusion was analyzed. The calculating model put forward can be regarded as a new technique for studying the crack and its expanding caused by inclusion tip. Then several numerical examples are given. | TAO Fangming(陶昉敏) TANG Renji(汤任基) | 2001 | Applied Mathematics and Mechanics(English Edition)2001，22，5： | 0 |
| 20 | Overexpressed PKM2 promotes macrophage phagocytosis and atherosclerosis显示文摘Background:The expression of pyruvate kinase muscle 2(PKM2)is augmented in macrophages of patients with atherosclerotic coronary artery disease.The role of PKM2 in atherosclerosis is to be determined.Methods:Global and myeloid cell-specific PKM2 knock-in mice with ApoE^(-/-)background(ApoE^(-/-),PKM2^(KI/KI)and Lyz2-cre,ApoE^(-/-),and PKM2^(flox/flox))were produced to evaluate the clinical significance of PKM2 in atherosclerosis development.Wild-type and PKM2 knock-in macrophages were isolated to assess the function of PKM2 in macrophage phagocytosis.Atherosclerotic mice were treated with PKM2 inhibitor shikonin(SKN)to evaluate the therapeutic potential of PKM2 suppression in atherosclerosis.Results:Oxidized low-density lipoprotein(oxLDL)upregulated PKM2 in macrophages.PKM2 in return promoted the uptake of oxLDL by macrophages.Overexpressed PKM2 accelerated atherosclerosis in mice.SKN blocked the progress of mouse atherosclerosis.Conclusions:PKM2 accelerates macrophage phagocytosis and atherosclerosis.Targeting PKM2 is a potential therapy for atherosclerosis. | Xiaochen Gai Fangming Liu Yuting Wu Baohui Zhang Bufu Tang Kezhuo Shang Lianmei Wang Haihong Zhang Yixin Chen Shuhui Yang Weiwei Deng Peng Li Jing Wang Hongbing Zhang | 2023 | Animal Models and Experimental Medicine2023，6，2： | 0 |
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