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| 1 | A novel m6A reader Prrc2a controls oligodendroglial specification and myelination显示文摘While N6-methyladenosine (m6A), the most abundant internal modification in eukaryotic mRNA, is linked to cell differentiation and tissue development, the biological significance of m6A modification in mammalian glial development remains unknown. Here, we identify a novel m6A reader, Prrc2a (Proline rich coiled-coil 2A), which controls oligodendrocyte specification and myelination. Nestin-Cre-mediated knockout of Prrc2a induces significant hypomyelination, decreased lifespan, as well as locomotive and cognitive defects in a mouse model. Further analyses reveal that Prrc2a is involved in oligodendrocyte progenitor cells (OPCs) proliferation and oligodendrocyte fate determination. Accordingly, oligodendroglial-lineage specific deletion of Prrc2a causes a similar phenotype of Nestin-Cre-mediated deletion. Combining transcriptome-wide RNA-seq, m6A-RIP-seq and Prrc2a RIP-seq analysis, we find that Olig2 is a critical downstream target gene of Prrc2a in oligodendrocyte development. Furthermore, Prrc2a stabilizes Olig2 mRNA through binding to a consensus GGACU motif in the Olig2 CDS (coding sequence) in an m6A-dependent manner. Interestingly, we also find that the m6A demethylase, Fto, erases the m6A modification of Olig2 mRNA and promotes its degradation. Together, our results indicate that Prrc2a plays an important role in oligodendrocyte specification through functioning as a novel m6A reader. These findings suggest a new avenue for the development of therapeutic strategies for hypomyelination-related neurological diseases. | Rong Wu Ang Li Baofa Sun Jian-Guang Sun Jinhua Zhang Ting Zhang Yusheng Chen Yujie Xiao Yuhao Gao Qingyang Zhang Jun Ma Xin Yang Yajin Liao Wei-Yi Lai Xiaolong Qi Shukun Wang Yousheng Shu Hai-Lin Wang Fengchao Wang Yun-Gui Yang Zengqiang Yuan | 2019 | Cell Research2019，29，1： | 32 |
| 2 | The apricot(Prunus armeniaca L.)genome elucidates Rosaceae evolution and beta-carotenoid synthesis显示文摘Apricots,scientifically known as Prunus armeniaca L,are drupes that resemble and are closely related to peaches or plums.As one of the top consumed fruits,apricots are widely grown worldwide except in Antarctica.A high-quality reference genome for apricot is still unavailable,which has become a handicap that has dramatically limited the elucidation of the associations of phenotypes with the genetic background,evolutionary diversity,and population diversity in apricot.DNA from P.armeniaca was used to generate a standard,size-selected library with an average DNA fragment size of~20 kb.The library was run on Sequel SMRT Cells,generating a total of 16.54 Gb of PacBio subreads(N50=13.55 kb).The high-quality P.armeniaca reference genome presented here was assembled using long-read single-molecule sequencing at approximately 70×coverage and 171×Illumina reads(40.46 Gb),combined with a genetic map for chromosome scaffolding.The assembled genome size was 221.9 Mb,with a contig NG50 size of 1.02 Mb.Scaffolds covering 92.88%of the assembled genome were anchored on eight chromosomes.Benchmarking Universal Single-Copy Orthologs analysis showed 98.0%complete genes.We predicted 30,436 protein-coding genes,and 38.28%of the genome was predicted to be repetitive.We found 981 contracted gene families,1324 expanded gene families and 2300 apricot-specific genes.The differentially expressed gene(DEG)analysis indicated that a change in the expression of the 9-cis-epoxycarotenoid dioxygenase(NCED)gene but not lycopene beta-cyclase(LcyB)gene results in a lowβ-carotenoid content in the white cultivar“Dabaixing”.This complete and highly contiguous P.armeniaca reference genome will be of help for future studies of resistance to plum pox virus(PPV)and the identification and characterization of important agronomic genes and breeding strategies in apricot. | Fengchao Jiang Junhuan Zhang Sen Wang Li Yang Yingfeng Luo Shenghan Gao Meiling Zhang Shuangyang Wu Songnian Hu Haoyuan Sun Yuzhu Wang | 2019 | Horticulture Research2019，6，1： | 9 |
| 3 | Pore Structure Characteristics and Permeability of Deep Sedimentary Rocks Determined by Mercury Intrusion Porosimetry显示文摘Pore structure characteristics of rock are a great concern for researchers and practitioners in rock mechanics and rock engineering fields. In this study, mercury intrusion porosimetry(MIP) was used to measure pore size distribution, as well as several important index parameters of pore structure, for seven common types of deep sedimentary rocks with a total of fifty rock samples. Results show a similar pore size distribution pattern of the rock samples in the same lithological group, but remarkable differences among different lithological groups. Among seven investigated rock types, mudstone has the smallest porosity of 3.37%, while conglomerate has the largest value of 18.8%. It is also found that the porosity of rock types with finer grain size is lower than those with coarser grain size. Meanwhile, a comparison of frequency distribution at ten intervals of pore-throat diameter among seven types of sedimentary rocks reveals that different rock types have different dominant pore-size ranges. Furthermore, permeability of the investigated sedimentary rock samples was derived based on MIP data using reported theoretical equations. Among seven rock types, mudstone has the lowest averaged permeability(3.64×10^(-6) mD) while conglomerate has the highest one(8.59×10^(-4) mD). From mudstone to conglomerate, rock permeability increases with an increase of grain size, with only an exception of siltstone which has a relatively larger porosity value. Finally, regression analysis show that there is a good fitting(R^2=0.95) between permeability and porosity which could be easily used to derive reliable permeability values of similar kinds of engineering rocks. | Na Zhang Manchao He Bo Zhang Fengchao Qiao Hailong Sheng Qinhong Hu | 2016 | Journal of Earth Science2016，27，4： | 9 |
| 4 | Fibroblasts in an endocardial fibroelastosis disease model mainly originate from mesenchymal derivatives of epicardium显示文摘 | Hui Zhang Xiuzhen Huang Kuo Liu Juan Tang Lingjuan He Wenjuan pu Qiaozhen Liu Yan Li Xueying Tian Yue Wang Libo Zhang Ying Yu Hongyan Wang Ronggui Hu Fengchao Wang Ting Chen Qing-Dong Wang Zengyong Qiao Li Zhang Kathy O Lui Bin Zhou | 2017 | Cell Research2017，27，9： | 6 |
| 5 | Type III interferon-induced CBFβinhibits HBV replication by hijacking HBx显示文摘Hepatitis B virus(HBV)and its associated chronic infection remain serious health threats worldwide.However,there is still no impactful approach for clinical treatment of hepatitis B patients.Therefore,developing a better understanding of the interactions between HBV and its host is particularly important.HBV infection has been reported to induce type-III but not type-I or type-II interferon(IFN).In this study,we identified CBFβ,an HIV enhancer,as an HBV restriction factor that is specifically induced by type-III IFN in the early stages of HBV infection.Type-III IFN-induced IL-10 played an important role in the production of CBFβ.Interestingly,the interaction between CBFβ-and HBV-encoded regulatory protein X(HBx)enhanced the stability of CBFβ,but notably blocked HBx-mediated promotion of HBV replication.CBFβexpression was lower in HBV patients than in healthy persons,and the addition of serum from HBV patients inhibited CBFβexpression in HepG2 cells.On the contrary,HBV via HBsAg inhibited type-III IFN-induced CBFβexpression and decreased the anti-HBV activity of type-III IFN,suggesting that HBV inhibits antiviral interferon-stimulated gene(ISG)expression and induces IFN resistance.Collectively,our results demonstrate that type-III IFN-triggered and IL-10-induced CBFβare crucial factors for inhibiting HBV replication,and the HBx–CBFβ–HBsAg axis reveals a new molecular mechanism of interaction between HBV and its hosts. | Fengchao Xu Hongxiao Song Qingfei Xiao Na Li Hong Zhang Genhong Cheng Guangyun Tan | 2019 | Cellular & Molecular Immunology2019，16，4： | 3 |
| 6 | Nature inspired hierarchical structures in nano-cellular epoxy/graphene-Fe3O4 nanocomposites with ultra-efficient EMI and robust mechanical strength显示文摘Hierarchical layered structures,whether in a compact form like nacre or a porous manner like bone,are well known for their combined features of high stiffness,strength,and lightweight,inspiring many man-made materials and structures for high performance applications.The use of nacre/bone like hierarchical structures in polymer nanocomposites can achieve excellent mechanical and functional properties with high filler volume fractions after carefully aligning functional nanofillers,although the fabrication and processing remain a great challenge.In this work,a bio-inspired lightweight nano-cellular epoxy/graphene-Fe_(3)O_(4) nanocomposite with high nanofiller loading of 75 wt.%was successfully fabricated by combining features from both nacre and bone structures,via a simple compression molding process together with an eco-friendly supercritical CO_(2) foaming process to achieve robust mechanical strength and excellent electromagnetic interference(EMI)shielding effectiveness(SE)simultaneously.Highly aligned graphene-Fe_(3)O_(4) nanoplatelets with well controlled nanoscale porous structures(52.6 nm)enabled both low density(1.26 g/cm^(3))and high specific EMI SE>5200 dB/cm^(2)/g,as well as preserved tensile strength of 67 MPa.This study provides a sustainable route to fabricate nature mimicked structures with high performance and high flexibility for a wide range of applications,from portable electronics to healthcare devices. | Xun Fan Fengchao Wang Qiang Gao Yu Zhang Fei Huang Ronglin Xiao Jianbin Qin Han Zhang Xuetao Shi Guangcheng Zhang | 2022 | Journal of Materials Science & Technology2022，，8： | 2 |
| 7 | Rice GLUTATHIONE PEROXIDASE1-mediated oxidation of bZIP68 positively regulates ABA-independent osmotic stress signaling显示文摘Osmotic stress caused by drought and high salinity is a significant environmental threat that limits plant growth and agricultural yield. Redox regulation plays an important role in plant stress responses, but the mechanisms by which plants perceive and transduce redox signals are still underexplored. Here, we report a critical function for the thiol peroxidase GPX1 in osmotic stress response in rice, where it serves as a redox sensor and transducer. GPX1 is quickly oxidized upon exposure to osmotic stress and forms an intramolecular disulfide bond, which is required for the activation of bZIP68, a VRE-like basic leucine zipper (bZIP) transcription factor involved in the ABA-independent osmotic stress response pathway. The disulfide exchange between GPX1 and bZIP68 induces homo-tetramerization of bZIP68 and thus positively regulates osmotic stress response by regulating osmotic-responsive gene expression. Furthermore, we discovered that the nuclear translocation of GPX1 is regulated by its acetylation under osmotic stress. Taken together, our findings not only uncover the redox regulation of the GPX1-bZIP68 module during osmotic stress but also highlight the coordination of protein acetylation and redox signaling in plant osmotic stress responses. | Heng Zhou Feng Zhang Fengchao Zhai Ye Su Ying Zhou Zhenglin Ge Priyadarshini Tilak Jürgen Eirich Iris Finkemeier Ling Fu Zongmin Li Jing Yang Wenbiao Shen Xingxing Yuan Yanjie Xie | 2022 | Molecular Plant2022，15，4： | 2 |
| 8 | Optical and electrical properties of a spiral LED filament显示文摘This paper introduces a new type of spiral white light-emitting diodes(WLED) filament with high luminous efficiency and uniform optical performance. The optical and thermal properties of the flexible filament were investigated at different stretching heights, namely 0, 1, 2, and 3 cm. The results indicated that the filament showed the best optical characteristics at the stretching height of 2 cm, because of good heat dissipation. In addition, the radiation temperature of the filament was inversely proportional to the output luminous flux. The reliability of the filament at a stretching height of 2 cm was also evaluated after 1000 h of use. The result demonstrated that the luminous flux decay of the bulb was only 0.85%. The flexible spiral WLED filament exhibiting high luminous flux and good reliability could be adapted to promote industrial development in the near future. | Liping Wang Jun Zou Bobo Yang Wenbo Li Yang Li Mingming Shi Wei Zhu Canyun Zhang Fengchao Wang Yujie Lin | 2018 | Journal of Semiconductors2018，39，2： | 2 |
| 9 | Study on the optimal concentration of topical mitomycin-C in preventing postlaminectomy epidural adhesion显示文摘 | Changhui Su Changjiang Yao Shenghua Lu Ailiang Zhang Xiaojian Cao Gaojun Teng Fengchao Zang | 2010 | European Journal of Pharmacology2010，，1： | 1 |
| 10 | Identificaiton of differentially expressed proteins of gamma-ray irradiated rat intestinal epithelial IEC-6 cells by two-dimensional gel electrophoresis and matric-assisted laser desorption/ionisation-time of flight mass spectrometry 显示文摘 | Zhang Bo Su Yongping Wang Fengchao | 2005 | Proteomics2005，5，2： | 1 |
| 11 | 3D printing of fine-grained aluminum alloys through extrusion-based additive manufacturing:Microstructure and property characterization显示文摘Additive manufacturing(AM)has the potential to transform manufacturing by enabling previously un-thinkable products,digital inventory and delivery,and distributed manufacturing.Here we presented an extrusion-based metal AM method(refer to“SoftTouch”depositionin thefiledpatent)thatis suitablefor making the metal feedstock flowable prior to the deposition through dynamic recrystallization induced grain refinement at elevated temperatures.The flowable metal was extruded out of the printer head like a paste for building dense metal parts with fine equiaxed grains and wrought mechanical properties.Off-the-shelf metal rods were used as feedstock and the printing process was completed in an open-air environment,avoiding pricy powders and costly inert or vacuum conditions.The resulting multi-layer de-posited 6061 aluminum alloys yield strength and ductility comparable to wrought 6061 aluminum alloys after the same T6 heat treatment.The extrusion-based metal AM method can also be advanced as green manufacturing technologies for fabricating novel alloys and composites,adding novel features to existing parts,repairing damaged metal parts,and welding advanced metals for supporting sustainable manufac-turing,in addition to being developed into a cost-effective manufacturing process for the fabrication of dense metal of complex structural forms. | Fengchao Liu Pingsha Dong Abdul Sayeed Khan Yuning Zhang Randy Cheng Alan Taub Zongyi Ma | 2023 | Journal of Materials Science & Technology2023，，8： | 1 |
| 12 | Early Cretaceous A-type Granites in the Dandong Area,NE China and its Geodynamical Implications显示文摘A-type granites,as an important petrologic sign to build up environmental recognition,were mainly formed in extensional tectonic settings.A biotite syenogranite from the Fenghuangshan pluton in Dandong,Liaoning Province gave SHRIMP zircon U-Pb ages of 122.5±1.6 Ma,124.9±1.7 Ma and 126.9±1.1 Ma.The monzogranites have SHRIMP zircon U-Pb ages of 118.2±1.6 Ma,128.1±1.7 Ma and 131.6±1.9 Ma,giving an emplacement age for the pluton in the Early Cretaceous.SiO_(2) is 65.48-74.49 wt%,whereas that of K_(2)O is 4.16-6.44 wt%,and that of Na_(2)O is 2.99-4.70 wt%.They also contain 13.24-15.76 wt% of Al_(2)O_(3),with an A/CNK ratio of 0.92-1.10,averaging 1.02.The alkalinity rate(AR)ranges from 2.68 to 5.12,and this range is within the AR of peraluminous type rocks.The granites of Fenghuangshan pluton are characterized by high contents of Na and K and low contents of Ca(thermophile element)and Mg,which are features of A-type granites.The(Na_(2)O+K_(2)O)-Fe_(2)O_(3)^(*)×5-(CaO+MgO)×5 discrimination diagram also shows that Fenghuangshan pluton is an A2-type granite.The above granite has zircon ε_(Hf)(t)values ranging from-17.06 to-9.09,with single-stage Hf model ages(T_(DM))of 1141 Ma to 1498 Ma and two-stage Hf crustal model ages(T_(DM)^(C))of 1762 Ma to 2263Ma.A comprehensive analysis suggests that the Fenghuangshan pluton might have been formed from the lithospheric-plate sliding during the late stages of evolution of hot rift structures and might have been closely associated with the tectonic settings of Mesozoic Eurasia and the ancient Pacific Plate. | SONG Yunhong HAO Libo YANG Fengchao ZHAO Dongfang ZHANG Zhehuan | 2021 | Acta Geologica Sinica(English Edition)2021，95，5： | 1 |
| 13 | The genome of the warm-season turfgrass African bermudagrass (Cynodon transvaalensis)显示文摘Cynodon species can be used for multiple purposes and have high economic and ecological significance.However,the genetic basis of the favorable agronomic traits of Cynodon species is poorly understood,partially due to the limited availability of genomic resources.In this study,we report a chromosome-scale genome assembly of a diploid Cynodon species,C.transvaalensis,obtained by combining Illumina and Nanopore sequencing,BioNano,and Hi-C.The assembly contains 282 scaffolds(~423.42 Mb,N50=5.37 Mb),which cover~93.2%of the estimated genome of C.transvaalensis(~454.4Mb).Furthermore,90.48%of the scaffolds(~383.08 Mb)were anchored to nine pseudomolecules,of which the largest was 60.78Mb in length.Evolutionary analysis along with transcriptome comparison provided a preliminary genomic basis for the adaptation of this species to tropical and/or subtropical climates,typically with dry summers.The genomic resources generated in this study will not only facilitate evolutionary studies of the Chloridoideae subfamily,in particular,the Cynodonteae tribe,but also facilitate functional genomic research and genetic breeding in Cynodon species for new leading turfgrass cultivars in the future. | Fengchao Cui Geli Taier Manli Li Xiaoxia Dai Nan Hang Xunzhong Zhang Xiangfeng Wang Kehua Wang | 2021 | Horticulture Research2021，8，1： | 1 |
| 14 | Reprogramming Glial Cells into Functional Neurons for Neuro-regeneration:Challenges and Promise显示文摘The capacity for neurogenesis in the adult mammalian brain is extremely limited and highly restricted to a few regions,which greatly hampers neuronal regeneration and functional restoration after neuronal loss caused by injury or disease.Meanwhile,transplantation of exogenous neuronal stem cells into the brain encounters several serious issues including immune rejection and the risk of tumorigenesis.Recent discoveries of direct reprogramming of endogenous glial cells into functional neurons have provided new opportunities for adult neuro-regeneration.Here,we extensively review the experimental findings of the direct conversion of glial cells to neurons in vitro and in vivo and discuss the remaining issues and challenges related to the glial subtypes and the specificity and efficiency of direct cell-reprograming,as well as the influence of the microenvironment.Although in situ glial cell reprogramming offers great potential for neuronal repair in the injured or diseased brain,it still needs a large amount of research to pave the way to therapeutic application. | Fengchao Wang Leping Cheng Xiaohui Zhang | 2021 | Neuroscience Bulletin2021，37，11： | 1 |
| 15 | Chlorohyd-rin Water Swellable Rubber Compatibilized by an Amphiphilic Graft Copolymer,l Synthesis and Characterization of Compatibilizer PVA-g-PBA显示文摘 | Zhang Guo Zhang Zhihao Xie Fengchao | 2000 | J Appl Polym Sci2000，75，： | 1 |
| 16 | Identification of differentially expressed proteins of gamma-ray irradiated rat intestinal epithelial IEC-6 cells by two-dimensional gel electrophoresis and matric-assisted laser desorption/ionisation-time of flight mass spectrometry 显示文摘 | ZHANG BO SU YONGPING WANG FENGCHAO et al | 2005 | Proteomics2005，5，2： | 1 |
| 17 | Differentiation of embryonic stem cells in adult bone marrow显示文摘Embryonic stem cells (ESCs) are a potential source of generating transplantable hematopoietic stem and progenitor cells,which in turn can serve as 'seed' cells for hematopoietic regeneration.In this study,we aimed to gauge the ability of mouse ESCs directly differentiating into hematopoietic cells in adult bone marrow (BM).To this end,we first derived a new mouse ESC line that constitutively expressed the green fluorescent protein (GFP) and then injected the ESCs into syngeneic BM via intra-tibia.The progeny of the transplanted ESCs were then analyzed at different time points after transplantation.Notably,however,most injected ESCs differentiated into non-hematopoietic cells in the BM whereas only a minority of the cells acquired hematopoietic cell surface markers.This study provides a strategy for evaluating the differentiation potential of ESCs in the BM micro-environment,thereby having important implications for the physiological maintenance and potential therapeutic applications of ESCs. | Yueying Li Jing He Fengchao Wang Zhenyu Ju Sheng Liu Yu Zhang Zhaohui Kou Yanfeng Liu Tao Cheng Shaorong Gao | 2010 | Journal of Genetics and Genomics2010，37，7： | 1 |
| 18 | Long-term survival of exogenous embryonic stem cells in adult bone marrow显示文摘 | Yueying Li Yanfeng Liu Jing He Fengchao Wang Sheng Liu Yu Zhang Zhaohui Kou Zhenyu JU Guoguang Zheng Jing Xu Weiping Yuan Shaorong Gao Tao Cheng | 2011 | Cell Research2011，21，7： | 1 |
| 19 | Capturing the hierarchically assorted modules of protein–protein interactions in the organized nucleome显示文摘Nuclear proteins are major constituents and key regulators of nucleome topological organization and manipulators of nuclear events.To decipher the global connectivity of nuclear proteins and the hierarchically organized modules of their interactions,we conducted two rounds of cross-linking mass spectrometry(XL-MS)analysis,one of which followed a quantitative double chemical cross-linking mass spectrometry(in vivo qXL-MS)workflow,and identified 24,140 unique crosslinks in total from the nuclei of soybean seedlings.This in vivo quantitative interactomics enabled the identification of 5340 crosslinks that can be converted into 1297 nuclear protein–protein interactions(PPIs),1220(94%)of which were non-confirmative(or novel)nuclear PPIs compared with those in repositories.There were 250 and 26 novel interactors of histones and the nucleolar box C/D small nucleolar ribonucleoprotein complex,respectively.Modulomic analysis of orthologous Arabidopsis PPIs produced 27 and 24 master nuclear PPI modules(NPIMs)that contain the condensate-forming protein(s)and the intrinsically disordered region–containing proteins,respectively.These NPIMs successfully captured previously reported nuclear protein complexes and nuclear bodies in the nucleus.Surprisingly,these NPIMs were hierarchically assorted into four higher-order communities in a nucleomic graph,including genome and nucleolus communities.This combinatorial pipeline of 4C quantitative interactomics and PPI network modularization revealed 17 ethylene-specific module variants that participate in a broad range of nuclear events.The pipeline was able to capture both nuclear protein complexes and nuclear bodies,construct the topological architectures of PPI modules and module variants in the nucleome,and probably map the protein compositions of biomolecular condensates. | Shuaijian Dai Shichang Liu Chen Zhou Fengchao Yu Guang Zhu Wenhao Zhang Haiteng Deng Al Burlingame Weichuan Yu Tingliang Wang Ning Li | 2023 | Molecular Plant2023，16，5： | 0 |
| 20 | The hydrophobic cluster on the surface of protein is the key structural basis for the SDS-resistance of chondroitinase VhChlABC显示文摘The application of chondroitinase requires consideration of the complex microenvironment of the target.Our previous research reported a marine-derived sodium dodecyl sulfate(SDS)-resistant chondroitinase VhChlABC.This study further investigated the mechanism of VhChlABC resistance to SDS.Focusing on the hydrophobic cluster on its strong hydrophilic surface,it was found that the reduction of hydrophobicity of surface residues Ala181,Met182,Met183,Ala184,Val185,and Ile305 significantly reduced the SDS resistance and stability.Molecular dynamics(MD)simulation and molecular docking analysis showed that I305G had more conformational flexibility around residue 305 than wild type(WT),which was more conducive to SDS insertion and binding.The affinity of A181G,M182A,M183A,V185A and I305G to SDS was significantly higher than that of WT.In conclusion,the surface hydrophobic microenvironment composed of six residues was the structural basis for SDS resistance.This feature could prevent the binding of SDS and the destruction of hydrophobic packaging by increasing the rigid conformation of protein and reducing the binding force of SDS-protein.The study provides a new idea for the rational design of SDS-resistant proteins and may further promote chondroitinase research in the targeted therapy of lung diseases under the pressure of pulmonary surfactant. | Juanjuan Su Hao Wu Chengying Yin Fengchao Zhang Feng Han Wengong Yu | 2024 | Marine Life Science & Technology2024，6，1： | 0 |
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