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| 1 | Immune Responses to the Attenuated Mycoplasma hyopneumoniae 168 Strain Vaccine by Intrapulmonic Immunization in Piglets显示文摘To investigate the immune responses to the attenuated Mycoplasma hyopneumoniae 168 strain vaccine, 8-15 d old piglets were immunized with M. hyopneumoniae 168 strain vaccine by intrapulmonic route. And the specific IgG antibody in serum, lymphoproliferation, IFNγ, and specific secretory IgA (SIgA) antibody in bronchoalveolar lavage fluid were detected on 30 and 60 d post-immunization (DPI), respectively. On 60 DPI, all the pigs except for those in health control group were challenged with a field M. hyopneumoniae strain JS. Necropsy was performed on 30 d post-challenge (DPC). The results showed that IFNγ and specific SIgA were stimulated on surface of respiratory tract after immunization. And peripheral blood mononuclear cells could also be proliferated about 1.81 and 2.12 fold on 30 and 60 DPI when stimulated by M. hyopneumoniae protein in vitro. However, no serum IgG antibody against M. hyopneumoniae was detected during the whole immune phage. After challenge, vaccinated pigs were observed with only very slight histological lesion in individual lobes. None of vaccinated pigs showed any clinical signs. While the unvaccinated pigs from challenge control group showed varying degrees of clinical sign and severe macroscopical lesion of mycoplasmal pneumonia of swine (MPS). The result suggested that the attenuated M. hyopneumoniae 168 strain vaccine inoculated by intrapulmonic route could activate the systemic cellular immunity, the local mucosal immunity and IFNγ secretion in respiratory tract to against M. hyopneumoniae infection in piglets. | FENG Zhi-xin, SHAO Guo-qing, LIU Mao-jun, WU Xu-su, ZHOU Yong-qi and GAN Yuan Key Laboratory of Animal Diseases Diagnostic and Immunology, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/National Center for Engineering Research of Veterinary Bio-Products, Ministry of Agriculture, Nanjing 210014, P.R.China | 2010 | Agricultural Sciences in China2010，9，3： | 22 |
| 2 | Expression of two types of acetylcholinesterase gene from the silkworm, Bombyx mori, in insect cells显示文摘编码乙酰胆碱酯酶(疼痛) 的二种类型的互补 DNA 从蚕被孤立, Bombyx 粗腐殖质 i。类型 1 (Bmace1 ) 并且类型 2 (Bmace2 ) ORF 分别地是在长度的 2052 和 1917 bp。两个都, Bmaces 和 C 终端的完全的 ORF 截断了形式在 polyhedrin 倡导者的控制下面被重新结合进 Bacmid baculovirus 向量并且在 Trichoplusia 表示了 ni (Tn-5B1-4 ) 房间。产生产品展出了疼痛活动和表示蛋白质的 glycosylation。抑制试金显示 ace2 类型酶比对由 eserine 和 paraoxon 的抑制的 ace1 类型酶更敏感。 | JIN-YAN SHANG YA-MING SHAO GUO-JUN LANG GAN YUAN ZHEN-HUA TANG CHUAN-XI ZHANG | 2007 | Insect Science2007，14，6： | 14 |
| 3 | Phosphorylcholine polymer nanocapsules prolong the circulation time and reduce the immunogenicity of therapeutic proteins显示文摘蛋白质治疗,治疗学的蛋白质在那里被交付对待混乱,为治疗疾病被认为最安全、很直接的途径。然而,它的应用被有效策略的少量高度为在他们的管理以后在 vivo 交付蛋白质和治疗学的蛋白质的快速的清理限制。这里,我们表明能显著地延长治疗学的蛋白质的发行量时间以及最小化他们的 immunogenicity 的新奇策略。这被与抵抗蛋白质吸附的 crosslinked phosphorylcholine 聚合物的薄层包含单个蛋白质分子完成。通过广泛的细胞的研究,我们证明 crosslinked phosphorylcholine 聚合物壳有效地阻止包含的蛋白质是由巨噬细胞的 phagocytosed,它在 vivo 在 nanoparticles 的清理起一个必要作用。而且,聚合物壳阻止包含的蛋白质被有免疫力的房间识别。作为结果,对治疗学的蛋白质的有免疫力的回答有效地被压制。这个工作描述一个可行方法延长循环时间并且减少治疗学的蛋白质的 immunogenicity,它可以在多样的疾病的治疗支持新奇蛋白质治疗的发展和申请。 | Sheng Liang Yang Liu Xin Jin Gan Liu Jing Wen Linlin Zhang Jie Li Xubo Yuan Irvin S.Y. Chen Wei Chen Hui Wang Linqi Shi Xinyuan Zhu Yunfeng Lu | 2016 | Nano Research2016，9，4： | 11 |
| 4 | Deep learning applied to two-dimensional color Doppler flow imaging ultrasound images significantly improves diagnostic performance in the classification of breast masses:a multicenter study显示文摘Background:The current deep learning diagnosis of breast masses is mainly reflected by the diagnosis of benign and malignant lesions.In China,breast masses are divided into four categories according to the treatment method:inflammatory masses,adenosis,benign tumors,and malignant tumors.These categorizations are important for guiding clinical treatment.In this study,we aimed to develop a convolutional neural network(CNN)for classification of these four breast mass types using ultrasound(US)images.Methods:Taking breast biopsy or pathological examinations as the reference standard,CNNs were used to establish models for the four-way classification of 3623 breast cancer patients from 13 centers.The patients were randomly divided into training and test groups(n=1810 vs.n=1813).Separate models were created for two-dimensional(2D)images only,2D and color Doppler flow imaging(2D-CDFI),and 2D-CDFI and pulsed wave Doppler(2D-CDFI-PW)images.The performance of these three models was compared using sensitivity,specificity,area under receiver operating characteristic curve(AUC),positive(PPV)and negative predictive values(NPV),positive(LR+)and negative likelihood ratios(LR-),and the performance of the 2D model was further compared between masses of different sizes with above statistical indicators,between images from different hospitals with AUC,and with the performance of 37 radiologists.Results:The accuracies of the 2D,2D-CDFI,and 2D-CDFI-PW models on the test set were 87.9%,89.2%,and 88.7%,respectively.The AUCs for classification of benign tumors,malignant tumors,inflammatory masses,and adenosis were 0.90,0.91,0.90,and 0.89,respectively(95%confidence intervals[CIs],0.87-0.91,0.89-0.92,0.87-0.91,and 0.86-0.90).The 2D-CDFI model showed better accuracy(89.2%)on the test set than the 2D(87.9%)and 2D-CDFI-PW(88.7%)models.The 2D model showed accuracy of 81.7%on breast masses≤1 cm and 82.3%on breast masses>1 cm;there was a significant difference between the two groups(P<0.001).The accuracy of the CNN classifications for the test set(89.2%)was significantly higher than that of all the radiologists(30%).Conclusions:The CNN may have high accuracy for classification of US images of breast masses and perform significantly better than human radiologists.Trial registration:Chictr.org,ChiCTR1900021375;http://gffzz62a03ee25e6f4a77hw9n6qopf9bf96pq6.ffgz.tsg.suse.edu.cn/showproj.aspx?proj=33139. | Teng-Fei Yu Wen He Cong-Gui Gan Ming-Chang Zhao Qiang Zhu Wei Zhang Hui Wang Yu-Kun Luo Fang Nie Li-Jun Yuan Yong Wang Yan-Li Guo Jian-Jun Yuan Li-Tao Ruan Yi-Cheng Wang Rui-Fang Zhang Hong-Xia Zhang Bin Ning Hai-Man Song Shuai Zheng Yi Li Yang Guang | 2021 | Chinese Medical Journal2021，，4： | 11 |
| 5 | Sirolimus induces apoptosis and reverses multidrug resistance in human osteosarcoma cells in vitro via increasing microRNA-34b expression显示文摘瞄准:多药抵抗在化疗提出一个批评瓶颈。在许多 chemoresistant 癌症给 mTOR 小径的起来规定,我们检验了是否 sirolimus (rapamycin ) ,第一个产生 mTOR 禁止者,可能导致人的 osteosarcoma (OS ) 房间 apoptosis 和增加到在 vitro.Methods 的 anticancer 药的 OS 房间的敏感:人的 OS 房间线 MG63/ADM 与 doxorubicin (ADM ) 独自或在联合与 sirolimus 被对待, gemcitabine (宝石) 或 methotrexate (MTX ) 。房间增长和 apoptosis 被检测分别地使用 CCK-8 试金和流动 cytometry。在房间的 MiRNAs 与 miRNA microarray 被分析。miR-34b 的目标基于 TargetScan 分析和酶记者试金被决定。相关 mRNA 和蛋白质的表示用 qRT-PCR 并且西方的弄污被测量。在 OS 病人的 40 件织物样品的 MiR-34, PAK1 和 ABCB1 层次用 qRT-PCR 并且在 situ 杂交 assays.Results 被分析:Sirolimus (1-100 nmol/L ) dose-dependently 压制了房间增长(IC 50=23.97 nmol/L ) 并且导致了 apoptosis。Sirolimus (10 nmol/L ) 显著地敏化房间到 anticancer 药,导致 ADM,宝石和 MTX 的减少的 IC 50 价值(从 25.48, 621.41 和 21.72 μ mol/L 到 4.93, 73.92 和 6.77 μ mol/L,分别地) 。治疗与 sirolimus,增加的 miR-34b 在房间由 7.5 的一个因素铺平。miR-34b 的 Upregulation 也导致了 apoptosis 并且,增加了房间的敏感到 anticancer 药有 miR-34b-AMO 的 transfection, miR-34b 的一个禁止者,颠倒了 sirolimus 的反增长效果。房间周期, apoptosis 和多重药抵抗, PAK1 和 ABCB1 的二个关键管理者,被表明是 miR-34b 的直接目标。在 OS 病人的 40 件织物样品,显著地更高的 miR-34 ISH 分数和 ABCB1 分数在 chemo 敏感的 group.Conclusion 被检测:Sirolimus 由与 PAK1 和 ABCB1 交往的起来调整的 miR-34b 在 vitro 增加人的 OS 房间的敏感到 anticancer 药。低 miR-34 水平是在 OS 病人的差的预后的指示物。 | Yan ZHOU Rui-hua ZHAO Kuo-Fu TSENG Kun-peng LI Zhi-gang LU Yuan LIU Kun HAN Zhi-hua GAN Shu-chenLIN Hai-yan HU Da-liu MIN | 2016 | Acta Pharmacologica Sinica2016，37，4： | 10 |
| 6 | Simulated Microgravity Conditions and Carbon Ion Irradiation Induce Spermatogenic Cell Apoptosis and Sperm DNA Damage显示文摘Objective To investigate the effect of simulated microgravity and carbon ion irradiation(CIR) on spermatogenic cell apoptosis and sperm DNA damage to the testis of male Swiss Webster mice,and assess the risk associated with space environment.Methods Sperm DNA damage indicated by DNA fragmentation index(DFI) and high DNA stainability(HDS) was measured by sperm chromatin structure assay(SCSA).Apoptosis of spermatogenic cells was detected by annexin V-propidium iodide assay.Bax(the expression levels of p53) and proliferating cell nuclear antigen(PCNA) were measured by immunoblotting;p53 and PCNA were located by immunohistology.Results HDS,DFI,apoptosis index,and the expression levels of p53 and Bax were detected to be significantly higher in the experimental groups(P<0.05) compared with those in the control group;however,the PCNA expression varied to a certain degree.p53-and PCNA-positive expression were detected in each group,mainly in relation to the spermatogonic cells and spermatocytes.Conclusion The findings of the present study demonstrated that simulated microgravity and CIR can induce spermatogenic cell apoptosis and sperm DNA damage.Sperm DNA damage may be one of the underlying mechanisms behind male fertility decline under space environment.These findings may provide a scientific basis for protecting astronauts and space traveler's health and safety. | LI Hong Yan ZHANG Hong MIAO Guo Ying XIE Yi SUN Chao DI Cui Xia LIU Yang LIU Yuan Yuan ZHANG Xin MA Xiao Fei XU Shuai GAN Lu ZHOU Xin | 2013 | Biomedical and Environmental Sciences2013，26，9： | 10 |
| 7 | Comparison of sentinel lymph node detection performances using blue dye in conjunction with indocyanine green or radioisotope in breast cancer patients: a prospective singlecenter randomized study显示文摘Objective:This randomized study aimed to compare the clinical efficacy between the novel dual tracer composed of indocyanine green(ICG)and blue dye(BD)and the conventional dual tracer composed of radioisotope and BD for sentinel lymph node(SLN)mapping in patients with breast cancer.Methods:This study enrolled 471 clinically lymph node-negative patients with primary breast cancer.All patients underwent mastectomy,and those undergoing sentinel lymph node biopsy(SLNB)were randomized to receive blue dye plus radioisotope(RB group)or BD plus ICG(IB group).The detection performances on SLN identification rate,positive SLN counts,detection sensitivity,and false-negative rate were compared between the two groups.Results:In the IB group,97%(194/200)of the patients who underwent the ICG and BD dual tracer injection showed fluorescentpositive lymphatic vessels within 2–5 min.The identification rate of SLNs was comparable between the IB group(99.0%,198/200)and the RB group(99.6%,270/271)(P=0.79).No significant differences were observed in the identification rate of metastatic SLNs(22.5%vs.22.9%,P>0.05,RB group vs.IB group,the same below),positive SLN counts(3.72±2.28 vs.3.91±2.13,P>0.05),positive metastatic SLN counts(0.38±0.84 vs.0.34±0.78,P>0.05),SLNB detection sensitivity(94.4%vs.92.5%,P>0.05),or false-negative rate(5.6%vs.7.5%,P>0.05)between the two groups.Conclusions:ICG can be used as a promising alternative tracer for radioisotope in SLN mapping,and when it is combined with BD in lymphangiography,it offers comparable detection sensitivity compared to the conventional lymphatic mapping strategies that are widely used in clinical practice. | Long Yuan Xiaowei Qi Yi Zhang Xinhua Yang Fan Zhang Linjun Fan Li Chen Kongyong Zhang Ling Zhong Yanling Li Sijie Gan Wenying Fu Jun Jiang | 2018 | Cancer Biology & Medicine2018，15，4： | 9 |
| 8 | Involvement of the Mitochondrion-dependent and the Endoplasmic Reticulum Stress-signaling Pathways in Isoliquiritigenin-induced Apoptosis of HeLa Cell显示文摘Objective Isoliquiritigenin(ISL),a licorice chalconoid,is considered to be a bioactive agent with chemopreventive potential.This study investigates the mechanisms involved in ISL-induced apoptosis in human cervical carcinoma HeLa cells.Methods Cell viability was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide(MTT)assay.Apoptosis was determined by flow cytometry using an Annexin V-FITC Apoptosis Detection Kit.The intracellular ROS levels were assessed using a 2,7-dichlorofluorescein probe assay.The mitochondrial membrane potential was measured with the dual-emission potential-sensitive probe 5,5',6,6'-tetra-chloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide(JC-1).The degradation of poly-ADP-ribose polymerase(PARP)protein,the phosphorylation of PKR-like ER kinase(PERK),the phosphorylation of the α-subunit of eukaryotic initiation factor 2(eIF2α),the expression of the 78 kD glucose-regulated protein(GRP 78),and the activation of caspase-12 were analyzed via western blot analysis.Results ISL significantly inhibited the proliferation,the increase in ROS levels and apoptotic rates of HeLa cells in a concentration-dependent manner.Moreover,ISL induced mitochondrial dysfunction,caspase activation,and PARP cleavage,which displayed features of mitochondria dependent on apoptotic signals.Besides,exposure of HeLa cells to ISL triggered endoplasmic reticulum(ER)stress,as indicated by the increase in p-eIF2α and GRP78 expression,ER stress-dependent apoptosis is caused by the activation of ER-specific caspase-12.Conclusion The findings from our study suggest that ISL-induced oxidative stress causes HeLa cell apoptosis via the mitochondrion-dependent and the ER stress-triggered signaling pathways. | YUAN Xuan ZHANG Bo GAN Lu WANG Zhen Hua YU Ba Cui LIU Liang Liang ZHENG Qiu Sheng WANGZhi Ping | 2013 | Biomedical and Environmental Sciences2013，26，4： | 8 |
| 9 | Elastoplastic Contact Mechanics Model of Rough Surface Based on Fractal Theory显示文摘Because the result of the MB fractal model contradicts with the classical contact mechanics, a revised elastoplastic contact model of a single asperity is developed based on fractal theory. The critical areas of a single asperity are scale dependent, with an increase in the contact load and contact area, a transition from elastic, elastoplastic to full plastic deformation takes place in this order. In considering the size distribution function, analytic expression between the total contact load and the real contact area on the contact surface is obtained. The elastic, elastoplastic and full plastic contact load are obtained by the critical elastic contact area of the biggest asperity and maximun contact area of a single asperity. The results show that a rough surface is firstly in elastic deformation. As the load increases, elastoplastic or full plastic deformation takes place. For constant characteristic length scale G, the slope of load-area relation is proportional to fractal dimension D. For constant fractal dimension D, the slope of load-area relation is inversely proportional to G. For constant D and G, the slope of load-area relation is inversely proportional to property of the material ? , namely with the same load, the material of rough surface is softer, and the total contact area is larger. The contact mechanics model provides a foundation for study of the friction, wear and seal performance of rough surfaces. | YUAN Yuan GAN Li LIU Kai YANG Xiaohui | 2017 | Chinese Journal of Mechanical Engineering2017，30，1： | 8 |
| 10 | High-Q resonances governed by the quasi-bound states in the continuum in all-dielectric metasurfaces显示文摘The realization of high-Q resonances in a silicon metasurface with various broken-symmetry blocks is reported. Theoretical analysis reveals that the sharp resonances in the metasurfaces originate from symmetry-protected bound in the continuum(BIC) and the magnetic dipole dominates these peculiar states. A smaller size of the defect in the broken-symmetry block gives rise to the resonance with a larger Q factor. Importantly, this relationship can be tuned by changing the structural parameter, resulting from the modulation of the topological configuration of BICs. Consequently, a Q factor of more than 3,000 can be easily achieved by optimizing dimensions of the nanostructure. At this sharp resonance, the intensity of the third harmonic generation signal in the patterned structure can be 368 times larger than that of the flat silicon film. The proposed strategy and underlying theory can open up new avenues to realize ultrasharp resonances, which may promote the development of the potential meta-devices for nonlinearity, lasing action, and sensing. | Cizhe Fang Qiyu Yang Qingchen Yuan Xuetao Gan Jianlin Zhao Yao Shao Yan Liu Genquan Han Yue Hao | 2021 | Opto-Electronic Advances2021，4，6： | 8 |
| 11 | Effect of H_2O(g)Content in Circulating Flue Gas on Iron Ore Sintering with Flue Gas Recirculation显示文摘The influence of H_2O(g) content in circulating flue gas on sintering was studied by simulating the flue gas circulating sintering.The results show that the burning speed of solid fuel and the heat transfer rate during sintering process are improved when the H_2O(g) content increases from 0 to 6%,which accelerates the sintering speed.However,when the H_2O(g) content exceeds 6%,the over-wet zone in sinter bed is thickened,which deteriorates the permeability of sintering bed.In addition,the magnetite content in sinter increases,while the acicular calcium ferrite content decreases.Accordingly,the sinter yield and tumble index decrease with excessive H_2O(g) content.To guarantee the yield and quality of sinter,the favourable H_2O(g) content in circulating flue gas should be controlled to be less than 6%. | Xu-ling CHEN Yun-song HUANG Min GAN Xiao-hui FAN Zhi-yuan YU Li-shun YUAN | 2015 | Journal of Iron and Steel Research(International)2015，22，12： | 8 |
| 12 | Risk factors of interstitial lung diseases in clinically amyopathic dermatomyositis显示文摘Background:Clinically amyopathic dermatomyositis(CADM)is a unique sub-type of idiopathic inflammatory myopathies with a high prevalence of interstitial lung disease(ILD).Poor prognosis of the patients was strongly associated with rapid progressive ILD.The aim of this study was to identify risk factors for prediction of different types of ILD in CADM.Methods:In this study,data of 108 inpatients with CADM were collected,including 87 with ILD.The baseline clinical data and laboratory parameters,including myositis-specific and associated antibodies and tumor-associated antigens were analyzed to identify risk factors for acute or subacute interstitial pneumonitis(A/SIP)and chronic interstitial pneumonitis(CIP).Results:In 87 patients with CADM-ILD,39(36.1%)were A/SIP,and 48(44.4%)were CIP.There were 22(20.4%)patients with asymptomatic ILD who were detected by routine high resolution computed tomography.Cytokeratin-19 fragment(CYFRA21-1)was significantly higher in CADM-ILD than that in CADM patients without ILD;carcinoembryonic antigen and neuron-specific enolase were significantly elevated in A/SIP than that in CIP.Patients with A/SIP had a higher positive rate of anti-melanoma differentiation-associated gene 5(MDA5),while patients with CIP had a higher positive rate of anti PL-12 and anti-Ro-52.Logistic regression analysis indicated that elevation of CYFRA21-1 was a risk factor for ILD,higher titer of anti-MDA5 indicated increased likelihood for A/SIP,and higher titer of anti-Ro-52 was also clearly associated with CIP.Conclusions:This study indicated that the prevalence of ILD was high in CADM.Asymptomatic ILD has been previously underestimated.Anti-MDA5 was a risk factor for the presence of A/SIP,and CYFRA21-1 was a risk factor for ILD. | Yu-Zhou Gan Li-Hua Zhang Lin Ma Feng Sun Yu-Hui Li Yuan An Zhan-Guo Li Hua Ye | 2020 | Chinese Medical Journal2020，，6： | 7 |
| 13 | Robust Multiparty Quantum Secret Key Sharing Over Two Collective-Noise Channels via Three-Photon Mixed States显示文摘 | WANG Zhang-Yin YUAN Hao GAO Gan Shou-Hua | 2006 | Communications in Theoretical Physics2006，46，4X： | 7 |
| 14 | Comparison of the short-term and long-term outcomes of laparoscopic hysterectomies and of abdominal hysterectomies: a case study of 4,895 patients in the Guangxi Zhuang Autonomous Region, China显示文摘Background: To evaluate the short-term and long-term outcomes after laparoscopic hysterectomy(LH) compared with abdominal hysterectomy(AH) in case of benign gynecological disease. Methods: A multi-center cohort retrospective comparative study of population among 4,895 hysterectomies(3,539 LH vs.1,356 AH) between 2007 and 2013 was involved. Operative time(OT), estimated blood loss(EBL), intra-operative and post-operative complications, passing flatus; days with indwelling catheter, questionnaires covering pelvic floor functions and sexual functions were assessed.Results: The EBL(174.1±157.4 vs. 263.1±183.2 cc, LH and AH groups, respectively), passing flatus(38.7±14.1 vs. 48.1±13.2 hours), days with indwelling catheter(1.5±0.6 vs. 2.2±0.8 days), use of analgesics(6.5% vs. 73.1%), intra-operative complication rate(2.4% vs. 4.1%), post-operative complication rate(2.3% vs. 5.7%), post-operative constipation(12.1% vs. 24.6%), mild and serious stress urinary incontinence(SUI) post-operative(P<0.001; P=0.014), and proportion of Female Sexual Functioning Index(FSFI) total score <26.55 post-operative(P<0.001) of the LH group were significantly less than those of AH group. There wereno significant differences in OT(106.5±34.5 vs. 106.2±40.3 min) between the two groups.Conclusions: LH is a safe and efficient operation for improving patients' long-term quality of life(Qo L), and LH is a cost-effectiveness procedure for treating benign gynecological disease. LH is superior to AH due to reduced EBL, reduced post-operative pain and earlier passing flatus. | Hongying He Zhijun Yang Dingyuan Zeng Jiangtao Fan Xiaoxia Hu Yuan Ye Hua Bai YanmingJiang Zhong Lin Zhiying Lei Xinlin Li Lian Li Jinghua Gan Ying Lan Xiongzhi Tang Danxia Wang Junsong Jiang Xiaoyan Wu Meiying Li Xiaoqing Ren Xiaomin Yang Mei Liu Qinmei Wang Fuyan jiang Li Li | 2016 | Chinese Journal of Cancer Research2016，28，2： | 6 |
| 15 | Azalomycin F_(4a) 2-ethylpentyl ester,a new macrocyclic lactone,from mangrove actinomycete Streptomyces sp.211726显示文摘Azalomycin F4a 2-ethylpentyl 酉旨,新 36 成员 macrocyclic 内酯抗菌素,从红树属植物放射菌类 Streptomyces sp 被孤立。211726。它的结构根据分光镜的数据被阐明。对人的冒号肿瘤房间 HCT-116 的复合显示出的用途广泛的抗真菌的活动和中等 cytotoxicity。 | Gan Jun Yuan Kui Hong Hai Peng Lin Jia Li | 2010 | Chinese Chemical Letters2010，21，8： | 6 |
| 16 | c-Jun N-terminal kinase 3 expression in the retina of ocular hypertension mice:a possible target to reduce ganglion cell apoptosis显示文摘Glaucoma, a type of optic neuropathy, is characterized by the loss of retinal ganglion cells. It remains controversial whether c-Jun N-terminal kinase(JNK) participates in the apoptosis of retinal ganglion cells in glaucoma. This study sought to explore a possible mechanism of action of JNK signaling pathway in glaucoma-induced retinal optic nerve damage. We established a mouse model of chronic ocular hypertension by reducing the aqueous humor followed by photocoagulation using the laser ignition method. Results showed significant pathological changes in the ocular tissues after the injury. Apoptosis of retinal ganglion cells increased with increased intraocular pressure, as did JNK3 m RNA expression in the retina. These data indicated that the increased expression of JNK3 m RNA was strongly associated with the increase in intraocular pressure in the retina, and correlated positively with the apoptosis of retinal ganglion cells. | Yue He Jie Chen Shu-guang Zhang Yuan-sheng Yuan Yan Li Hong-bin Lv Jin-hua Gan | 2015 | Neural Regeneration Research2015，10，3： | 6 |
| 17 | Cholesterol-tuned liposomal membrane rigidity directs tumor penetration and anti-tumor effect显示文摘Recently, liposomes have been widely used in cancer therapeutics, but their anti-tumor effects are suboptimal due to limited tumor penetration. To solve this problem, researchers have made significant efforts to optimize liposomal diameters and potentials, but little attention has been paid to liposomal membrane rigidity. Herein, we sought to demonstrate the effects of cholesterol-tuned liposomal membrane rigidity on tumor penetration and anti-tumor effects. In this study, liposomes composed of hydrogenated soybean phospholipids(HSPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000](DSPE-PEG2000) and different concentrations of cholesterol were prepared. It was revealed that liposomal membrane rigidity decreased with the addition of cholesterol. Moderate cholesterol content conferred excellent diffusivity to liposomes in simulated diffusion medium, while excessive cholesterol limited the diffusion process. We concluded that the differences of the diffusion rates likely stemmed from the alterations in liposomal membrane rigidity, with moderate rigidity leading to improved diffusion. Next, the in vitro tumor penetration and the in vivo anti-tumor effects were analyzed. The results showed that liposomes with moderate rigidity gained excellent tumor penetration and enhanced anti-tumor effects. These findings illustrate a feasible and effective way to improve tumor penetration and therapeutic efficacy of liposomes by changing the cholesterol content, and highlight the importance of liposomal membrane rigidity. | Hangyi Wu Miaorong Yu Yunqiu Miao Shufang He Zhuo Dai Wenyi Song Yuan Liu Sha Song Ejaj Ahmad Dongkai Wang Yong Gan | 2019 | Acta Pharmaceutica Sinica B2019，9，4： | 5 |
| 18 | Establishment and functional characterization of the reversibly immortalized mouse glomerular podocytes(imPODs)显示文摘Glomerular podocytes are highly specialized epithelial cells and play an essential role in establishing the selective permeability of the glomerular filtration barrier of kidney.Maintaining the viability and structural integrity of podocytes is critical to the clinical management of glomerular diseases,which requires a thorough understanding of podocyte cell biology.As mature podocytes lose proliferative capacity,a conditionally SV40 mutant tsA58-immortalized mouse podocyte line(designated as tsPC)was established from the Immortomouse over 20 years ago.However,the utility of the tsPC cells is hampered by the practical inconvenience of culturing these cells.In this study,we establish a user-friendly and reversibly-immortalized mouse podocyte line(designated as imPOD),on the basis of the tsPC cells by stably expressing the wildtype SV40 T-antigen,which is flanked with FRT sites.We show the imPOD cells exhibit long-term high proliferative activity,which can be effectively reversed by FLP recombinase.The imPOD cells express most podocyte-related markers,including WT-1,Nephrin,Tubulin and Vinculin,but not differentiation marker Synaptopodin.The imPOD cells do not form tumor-like masses in vivo.We further demonstrate that TGFb1 induces a podocyte injury-like response in the FLP-reverted imPOD cells by suppressing the expression of slit diaphragm-associated proteins P-Cadherin and ZO-1 and upregulating the expression of mesenchymal markers,a-SMA,Vimentin and Nestin,as well as fibrogenic factors CTGF and Col1a1.Collectively,our results strongly demonstrate that the newly engineered im-POD cells should be a valuable tool to study podocyte biology both under normal and under pathological conditions. | Xinyi Yu Liqun Chen Ke Wu Shujuan Yan Ruyi Zhang Chen Zhao Zongyue Zeng Yi Shu Shifeng Huang Jiayan Lei Xiaojuan Ji Chengfu Yuan Linghuan Zhang Yixiao Feng Wei Liu Bo Huang Bo Zhang Wenping Luo Xi Wang Bo Liu Rex C.Haydon Hue H.Luu Tong-Chuan He Hua Gan | 2018 | Genes & Diseases2018，5，2： | 5 |
| 19 | A Self-certified Cross-Cluster Asymmetric Group Key Agreement for Wireless Sensor Networks显示文摘Wireless sensor networks have some obvious characteristics, such as communication range is limited, computing power is limited and energy is limited. Group key agreement in this environment requires a cross-cluster, computation and communication overhead are lightweight and highly safe group key agreement protocol. Aiming at these demands, the paper proposes a Self-certified cross-cluster Asymmetric group key agreement(SC-AGKA). To establish a lightweight and efficient group communication channel among sensor nodes. According to the cluster head as the bridge node to realize the sensor nodes in different cluster have the same group key information, and negotiate a pair of asymmetric group keys to realize the cross cluster secure communication. The group communication adopts asymmetric encryption mechanism. It realizes the group security communication mechanism of message sender unconstraint. The asymmetric group key agreement has the key self-certified, which does not need additional rounds to verify the correctness of group key. Proven and analysis show that the proposed protocol has the advantages of in security and energy consumption. | ZHANG Qikun LI Yongjiao ZHANG Quanxin YUAN Junling WANG Ruifang GAN Yong TAN Yu'an | 2019 | Chinese Journal of Electronics2019，28，2： | 5 |
| 20 | Protective Effects of Shikonin on Brain Injury Induced by Carbon Ion Beam Irradiation in Mice显示文摘Radiation encephalopathy is the main complication of cranial radiotherapy.It can cause necrosis of brain tissue and cognitive dysfunction.Our previous work had proved that a natural antioxidant shikonin possessed protective effect on cerebral ischemic injury.Here we investigated the effects of shikonin on carbon ion beam induced radiation brain injury in mice.Pretreatment | GAN Lu WANG Zhen Hua ZHANG Hong ZHOU Rong SUN Chao LIU Yang SI Jing LIU Yuan Yuan WANG Zhen Guo | 2015 | Biomedical and Environmental Sciences2015，28，2： | 5 |
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