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| 1 | CRISPR-Cas9-mediated genome editing in one blastomere of two-cell embryos reveals a novel Tet3 function in regulating neocortical development显示文摘学习必要基因的早功能是在发展生物学的一个重要、挑战性的问题。这里,我们为很快在二房间的阶段胚胎的一个分裂球导致 CRISPR-Cas9-mediated 变化建立了一个方法,称为的 2 房间 embryo-CRISPR-Cas9 注射(2CC ) ,学习在里面必需品的 vivo 功能(或未知) 在创始人的基因妄想的鼠标。由注射指向兴趣的基因进的 Cre mRNA 和 CRISPR-Cas9 荧光灯记者老鼠, 2CC 方法能在不同发展阶段跟踪野类型、变异的房间,为变异的房间的 phenotypic 分析的出现内部控制。用这个方法,我们在在发展中的鼠标调整有刺激性、禁止的 synaptic 传播识别了必要基因 Tet3 的新奇函数服的外皮。由产生妄想的变异的老鼠, 2CC 方法允许在多重纸巾和房间的基因功能的快速的屏蔽录入妄想的老鼠创始人,显著地扩展基因工具的当前的一套设备。 | Wang, Lingbo Li, Min-Yin Qu, Chao Miao, Wan-Ying Yin, Qi Liao, Jiaoyang Cao, Hua-Teng Huang, Min Wang, Kai Zuo, Erwei Peng, Guangdun Zhang, Shu-Xin Chen, Guodong Li, Qing Tang, Ke Yu, Qian Li, Zhoujie Wong, Catherine C. L. Xu, Guoliang Jing, Naihe Yu, Xiang Li, Jinsong | 2017 | Cell Research2017，27，6： | 5 |
| 2 | Wheat breeding history reveals synergistic selection of pleiotropic genomic sites for plant architecture and grain yield显示文摘Diversity surveys of crop germplasm are important for gaining insights into the genomic basis for plant architecture and grain yield improvement,which is still poorly understood in wheat.In this study,we exome sequenced 287 wheat accessions that were collected in the past 100 years.Population genetics analysis identified that 6.7%of the wheat genome falls within the selective sweeps between landraces and cultivars,which harbors the genes known for yield improvement.These regions were asymmetrically distributed on the A and B subgenomes with regulatory genes being favorably selected.Genome-wide association study(GWAS)identified genomic loci associated with traits for yield potential,and two underlying genes,TaARF12 encoding an auxin response factor and TaDEP1 encoding the G-proteinγ-subunit,were located and characterized to pleiotropically regulate both plant height and grain weight.Elite single-nucleotide haplotypes with increased allele frequency in cultivars relative to the landraces were identified and found to have accumulated over the course of breeding.Interestingly,we found that TaARF12 and TaDEP1 function in epistasis with the classical plant height Rht-1 locus,leading to propose a“Green Revolution”-based working model for historical wheat breeding.Collectively,our study identifies selection signatures that fine-tune the gibberellin pathway during modern wheat breeding and provides a wealth of genomic diversity resources for the wheat research community. | Aili Li Chenyang Hao Zhenyu Wang Shuaifeng Geng Meiling Jia Fang Wang Xiang Han Xingchen Kong Lingjie Yin Shu Tao Zhongyin Deng Ruyi Liao Guoliang Sun Ke Wang Xingguo Ye Chengzhi Jiao Hongfeng Lu Yun Zhou Dengcai Liu Xiangdong Fu Xueyong Zhang Long Mao | 2022 | Molecular Plant2022，15，3： | 4 |
| 3 | Experimental and computational investigations of LaNi(5-x)Alx (x = 0,0.25, 0.5, 0.75 and 1.0) tritium-storage alloys显示文摘Although already scientists in recent years have reported some experimental and theoretical results of LaNi-Al series of tritium-storage alloys, several key aspects remain the subject of considerable debate. In an effort to interpret some of these unknowns, we have performed experimental and theoretical investigations for LaNi_(5-x)Al_x(x = 0, 0.25, 0.5, 0.75 and 1.0) tritium-storage alloys. Firstly, the XRD characterization indicates that the unit cell volumes of LaNi_(5-x)Al_x increase with Al content in alloys. Secondly, the PCisotherm measurement of LaNi_(5-x)Al_xalloys shows that their hydrogen absorption/desorption plateau pressures reduce with the increase of Al content while their plateau widths narrow simultaneously. The deuterium absorption/desorption plateaus have a similar trend to hydrogen's except for their plateaus being higher than hydrogen's. To explain the above experimental findings, a series of calculations based on density functional theory(DFT) and frozen phonon approach have been performed. The results manifest that:(1) the partial substitutions of Al for Ni reduce the hydrogen formation energies of LaNi_(5-x)Al_xH and the number of available interstitial sites, and therefore lead to the absorption/desorption plateau pressures being reduced and the plateau widths being narrowed down at the same experimental temperatures;(2) the covalent interaction between H and Ni is an important factor for estimating the stability of LaNi_(5-x)Al_x-H system;(3) since the calculated enthalpy change H is generally more accurate than the calculated entropy change S with respect to the corresponding experimental value for each LaNi_(5-x)Al_xH(or D), the curves of H vs. hydrogen storage capacity instead of Van't Hoff relation, can be used to predict the experimental plateau pressures of LaNi_(5-x)Al_x-H(D or T) at a given temperature;(4) the hydrogen isotope effect of LaNi_(5-x)Al_x-H(D or T) system can be quantitatively described as a linearity relation between ⊿ZPE + ⊿H^(vib) and 1/√mQ(Q = H, D, T). From the good agreement between the predicted and experimental ln(P_H/P_0) and ln(P_D/P_0), it is deduced that predicting ln(P_T/P_0) of LaNi_(5-x)Al_x T is feasible. The procedure of pre-computing and comparing curves of H vs. hydrogen storage capacity proposed in this paper provided an attractive tool to increase the efficiency of experimental alloying design of hydrogen(deuterium or tritium) storage materials. | Guoliang Liu Demin Chen Yuanming Wang Ke Yang | 2018 | Journal of Materials Science & Technology2018，34，9： | 3 |
| 4 | Electrochemical Lithium Storage Performance of Molten Salt Derived V_(2)SnC MAX Phase显示文摘MAX phases are gaining attention as precursors of two-dimensional MXenes that are intensively pursued in applications for electrochemical energy storage.Here,we report the preparation of V_(2)SnC MAX phase by the molten salt method.V_(2)SnC is investigated as a lithium storage anode,showing a high gravimetric capacity of 490 mAh g−1 and volumetric capacity of 570 mAh cm^(−3) as well as superior rate performance of 95 mAh g^(−1)(110 mAh cm^(−3))at 50 C,surpassing the ever-reported performance of MAX phase anodes.Sup-ported by operando X-ray diffraction and density functional theory,a charge storage mechanism with dual redox reaction is proposed with a Sn-Li(de)alloying reaction that occurs at the edge sites of V_(2)SnC particles where Sn atoms are exposed to the electrolyte followed by a redox reaction that occurs at V_(2)C layers with Li.This study offers promise of using MAX phases with M-site and A-site elements that are redox active as high-rate lithium storage materials. | Youbing Li Guoliang Ma Hui Shao Peng Xiao Jun Lu Jin Xu Jinrong Hou Ke Chen Xiao Zhang Mian Li Per OÅPersson Lars Hultman Per Eklund Shiyu Du Zhifang Chai Zhengren Huang Na Jin Jiwei Ma Ying Liu Zifeng Lin Qing Huang | 2021 | Nano-Micro Letters2021，13，10： | 2 |
| 5 | Detection of the Pandemic H1N1/2009 Influenza A Virus by a Highly Sensitive Quantitative Real-time Reverse-transcription Polymerase Chain Reaction Assay显示文摘A quantitative real time reverse-transcription polymerase chain reaction (qRT-PCR) assay with specific primers recommended by the World Health Organization (WHO) has been widely used successfully for detection and monitoring of the pandemic H1N1/2009 influenza A virus. In this study, we report the design and characterization of a novel set of primers to be used in a qRT-PCR assay for detecting the pandemic H1N1/2009 virus. The newly designed primers target three regions that are highly conserved among the hemagglutinin (HA) genes of the pandemic H1N1/2009 viruses and are different from those targeted by the WHO-recommended primers. The qRT-PCR assays with the newly designed primers are highly specific, and as specific as the WHO-recommended primers for detecting pandemic H1N1/2009 viruses and other influenza viruses including influenza B viruses and influenza A viruses of human, swine, and raccoon dog origin. Furthermore, the qRT-PCR assays with the newly designed primers appeared to be at least 10-fold more sensitive than those with the WHO-recommended primers as the detection limits of the assays with our primers and the WHO-recommended primers were 2.5 and 25 copies of target RNA per reaction, respectively. When tested with 83 clinical samples, 32 were detected to be positive using the qRT-PCR assays with our designed primers, while only 25 were positive by the assays with the WHO-recommended primers. These results suggest that the qRT-PCR system with the newly designed primers represent a highly sensitive assay for diagnosis of the pandemic H1N1/2009 virus infection. | Zhu Yang Guoliang Mao Yujun Yuan-Chuan Chen Chengjing Liu Jun Luo Xihan Li Ke Zen Yanjun Pang Jianguo Wu Fenyong Liu | 2013 | Virologica Sinica2013，28，1： | 2 |
| 6 | Perturbation analysis for a class of fuzzy linear systems显示文摘 | Wang Ke Chen Guoliang Wei Yimin | 2009 | Journal of Computational and Applied Mathematics2009，224，： | 1 |
| 7 | Construction and Application of DNAzyme-based Nanodevices显示文摘The development of stimuli-responsive nanodevices with high efficiency and specificity is very important in biosensing,drug delivery,and so on.DNAzymes are a class of DNA molecules with the specific catalytic activity.Owing to their unique catalytic activity and easy design and synthesis,the construction and application of DNAzymes-based nanodevices have attracted much attention in recent years.In this review,the classification and properties of DNAzyme are first introduced.The construction of several common kinds of DNAzyme-based nanodevices,such as DNA motors,signal amplifiers,and logic gates,is then systematically summarized.We also introduce the application of DNAzyme-based nanodevices in sensing and therapeutic fields.In addition,current limitations and future directions are discussed. | WANG Bo WANG Menghui PENG Fangqi FU Xiaoyi WEN Mei SHI Yuyan CHEN Mei KE Guoliang ZHANG Xiao-Bing | 2023 | Chemical Research in Chinese Universities2023，39，1： | 1 |
| 8 | Dust emission inventory in Northern China 显示文摘 | Xuan Jie Liu Guoliang Du Ke | 2000 | Atmospheric Enviromnent2000，34，： | 1 |
| 9 | Purification of CVD synthesized single-wall carbon nanotubes by different acid oxidation treatments显示文摘 | Yu Li Xiaobin Zhang Junhang Luo Wanzhen Huang Jipeng Cheng Zhiqiang Luo Ting Li Fu Liu Guoliang Xu Xiaoxing Ke Lin Li and Herman J Geise | 2004 | Nanotechnology2004，15，： | 1 |
| 10 | Nucleic acids analysis显示文摘Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nucleic acids and nucleic acids-based analysis have been widely applied in biological studies, clinical diagnosis, environmental analysis, food safety and forensic analysis.During the past decades, the field of nucleic acids analysis has been rapidly advancing with many technological breakthroughs.In this review, we focus on the methods developed for analyzing nucleic acids, nucleic acids-based analysis, device for nucleic acids analysis, and applications of nucleic acids analysis. The representative strategies for the development of new nucleic acids analysis in this field are summarized, and key advantages and possible limitations are discussed. Finally, a brief perspective on existing challenges and further research development is provided. | Yongxi Zhao Xiaolei Zuo Qian Li Feng Chen Yan-Ru Chen Jinqi Deng Da Han Changlong Hao Fujian Huang Yanyi Huang Guoliang Ke Hua Kuang Fan Li Jiang Li Min Li Na Li Zhenyu Lin Dingbin Liu Juewen Liu Libing Liu Xiaoguo Liu Chunhua Lu Fang Luo Xiuhai Mao Jiashu Sun Bo Tang Fei Wang Jianbin Wang Lihua Wang Shu Wang Lingling Wu Zai-Sheng Wu Fan Xia Chuanlai Xu Yang Yang Bi-Feng Yuan Quan Yuan Chao Zhang Zhi Zhu Chaoyong Yang Xiao-Bing Zhang Huanghao Yang Weihong Tan Chunhai Fan | 2021 | Science China Chemistry2021，64，2： | 1 |
| 11 | Dust emission inventory in Northern China 显示文摘 | Xuan Jie Guoliang Liu Ke Du | 2000 | Atmospheric Environment2000，34，： | 1 |
| 12 | Analysis of computational time of simple estimation of distribution algorithms显示文摘 | Chen Tianshi Tang Ke Chen Guoliang | 2010 | IEEE Transactions on Evolutionary Computation2010，14，1： | 1 |
| 13 | Analysis of computational time of simple estimation of distribution algorithms显示文摘 | Chen Tianshi Tang Ke Chen Guoliang | 2010 | IEEE Trans on Evolutionary Computation2010，14，1： | 1 |
| 14 | Population-Based Algorithm Portfolios for Numerical Optimi-zation 显示文摘 | Fei Peng Ke Tang Guoliang Chen | 2010 | IEEE Transactions on EvolutionaryComputation2010，14，5： | 1 |
| 15 | Analysis of computational time of simple estimation of distribution algorithms 显示文摘 | CI-IEN TIANSHI TANG KE CHEN GUOLIANG | 2010 | IEEE Transactions on Evolutionary Computation2010，14，1： | 1 |
| 16 | The soft glumes of common wheat are sterile-lemmas as determined by the domestication gene Q显示文摘The Q gene in common wheat encodes an APETALA2(AP2) transcription factor that causes the free threshing attribute. Wheat spikelets bearing several florets are subtended by a pair of soft glumes that allow free liberation of seeds. In wild species, the glumes are tough and rigid,making threshing difficult. However, the nature of these 'soft glumes', caused by the domestication allele Q is not clear. Here, we found that over expression of Q in common wheat leads to homeotic florets at glume positions. We provide phenotypic, microscopy, and marker genes evidence to demonstrate that the soft glumes of common wheat are in fact lemma-like organs, or so-called sterile-lemmas. By comparing the structures subtending spikelets in wheat and other crops such as rice and maize, we found that AP2 genes may play conserved functions in grasses by manipulating vestigial structures, such as floret-derived soft glumes in wheat and empty glumes in rice. Conversion of these seemingly vegetative organs to reproductive organs may be useful in yield improvement of crop species. | Gaoyuan Song Guoliang Sun Xingchen Kong Meiling Jia Ke Wang Xingguo Ye Yun Zhou Shuaifeng Geng Long Mao Aili Li | 2019 | The Crop Journal2019，7，1： | 1 |
| 17 | Cross-linked hole transport layers for high-efficiency perovskite tandem solar cells显示文摘Perovskite tandem solar cells have recently received extensive attention due to their promise of achieving power conversion efficiency(PCE)beyond the limits of single-junction cells.However,their performance is still largely constrained by the widebandgap perovskite solar cells which show considerable open-circuit voltage(VOC)losses.Here,we increase the VOCand PCE of wide-bandgap perovskite solar cells by changing the hole transport layer(HTL)from commonly used poly(bis(4-phenyl)(2,4,6-trimethylphenyl)amine)(PTAA)to in-situ cross-linked small molecule N_(4),N_(4)′-di(naphthalen-1-yl)-N_(4),N_(4)′-bis(4-vinylphenyl)biphenyl-4,4′-diamine(VNPB).The stronger interaction and lower trap density at the VNPB/perovskite interface improve the PCE and stability of wide-bandgap perovskite solar cells.By using the cross-linked HTL for front wide-bandgap subcells,PCEs of 24.9%and 25.4%have been achieved in perovskite/perovskite and perovskite/silicon tandem solar cells,respectively.The results demonstrate that cross-linkable small molecules are promising for high-efficiency and cost-effective perovskite tandem photovoltaic devices. | Yurui Wang Shuai Gu Guoliang Liu Liping Zhang Zhou Liu Renxing Lin Ke Xiao Xin Luo Jianhua Shi Junling Du Fanying Meng Ludong Li Zhengxin Liu Hairen Tan | 2021 | Science China Chemistry2021，64，11： | 1 |
| 18 | Determination of the predictive factors for diagnostic positivity of nucleic acid amplification tests for diagnosing pulmonary tuberculosis显示文摘Background:Tuberculosis(TB)remains a major threat to human health,and TB diagnostic methods remain unsatisfactory.Nucleic acid amplification tests(NAATs)show higher sensitivity compared with culture for the diagnosis of pulmonary TB(PTB).However,NAATs are expensive and cannot be easily implemented outside major medical centers.To improve the sensitivity of NAATs for PTB diagnosis,we investigated the predictive factors that might optimize NAAT utilization.Methods:A total of 1263 patients with suspected PTB were enrolled for evaluation.The sensitivity,specificity,and accuracy of methods including smear-microbiology,culture of Mtb and NAAT for Mycobacterium tuberculosis(Mtb)detection in sputum and bronchoalveolar lavage fluid samples were compared.Odds ratios and 95%confidence intervals were used to assess variables that might be associated with positive NAAT results for sputum and bronchoalveolar lavage fluid from patients with suspected PTB.Results:NAAT showed higher sensitivity for Mtb detection(61.1%)when compared with smear(9.0%)and Mtb culture(47.8%).We found that an elevated erythrocyte sedimentation rate,the presence of cavities,and positive interferon-��release assay(IGRA)results were indicative of positive Mtb detection by NAAT.Moreover,individuals who had all three of these characteristics showed an 86%diagnostic positivity for PTB from Mtb detection by NAAT.Conclusions:Our study suggests that an elevated erythrocyte sedimentation rate,a positive IGRA result,and the presence of pulmonary cavities are helpful factors for predicting positive Mtb detection by NAAT.Patients with the three positive clinical markers should undergo NAAT for Mtb detection because they are the most likely individuals to be bacteriologically confirmed as having TB. | Xingfang Hou Qinglong Guo Qiao Lin Taosheng Ye Jing Bi Juan Liang Ke Yang Yuan Zhou Juanjuan Zhang Zhihang Liang Xuefeng Zhou Gengwei Zhang Xiangdong Fu Hongjian Zhong Guoliang Zhang | 2022 | Infectious Medicine2022，1，1： | 0 |
| 19 | Experimental Study on Gas Flow Uniformity in a Diesel Particulate Filter Carrier显示文摘A Diesel Particulate Filter(DPF)is a critical device for diesel engine exhaust products treatment.When using active-regeneration purification methods,on the one hand,a spatially irregular gas flow can produce relatively high local temperatures,potentially resulting in damage to the carrier;On the other hand,the internal temperature field can also undergo significant changes contributing to increase this risk.This study explores the gas flow uniformity in a DPF carrier and the related temperature behavior under drop-to-idle(DTI)condition by means of bench tests.It is shown that the considered silicon carbide carrier exhibits good flow uniformity,with a temperature deviation of no more than 2%with respect to the same radius measurement point at the outlet during the regeneration stage.In the DTI test,the temperature is relatively high within r/2 near the outlet end,where the maximum temperature peak occurs,and the maximum radial temperature gradient is located between r/2 and the edge.Both these quantities grow as the soot load increases,thereby making the risk of carrier burnout greater.Finally,it is shown that the soot load limit of the silicon carbide DPF can be extended to 11 g/L,which reduces the frequency of active regeneration by approximately 40%compared to a cordierite DPF. | Zhengyong Wang Jianhua Zhang Guoliang Su Peixing Yang Xiantao Fan Shuzhan Bai Ke Sun Guihua Wang | 2024 | Fluid Dynamics & Materials Processing2024，20，1： | 0 |
| 20 | Photoactivatable Cell-Surface DNAzyme Sensor for Real-Time Monitoring of the Metal Ion-Induced AβAggregation显示文摘Precisely monitoring the distribution and local fluctuation of metal ions,which play vital roles in many physiological and pathological processes,contributes largely to the study of metal ion-induced pathogenesis.However,current reactive fluorescent sensors can hardly realize the monitoring of metal ions in situ due to the premature activation and aimless dispersion of the sensors,which hinder their applications in biological samples.Herein,we report a photoactivatable cell-surface DNAzyme sensor to monitor metal ions in the microenvironment of living cells or tissues with high spatial resolution and temporal control.The sensor can efficiently anchor to the cell membrane through amphipathic interaction,and the activity of the sensor can be restored after the irradiation by light,which avoids false positive signals from the reaction with metal cofactors during delivery.First,the properties of the sensor were investigated either in buffer or on amodel cell surface,inwhich the sensor responded to Zn2+with superior temporal and spatial resolution under light activation.Then,the sensor was applied tomonitor the dynamic changes ofmetal ions during the formation of Aβaggregates in the cellular microenvironment of brain cells and brain tissues.This strategy provides a potential tool to understand the roles ofmetal ions in the progression of diseases,such as Alzheimer’s disease. | Lu Liu Mei Zhang Qiming Rong Mengyi Xiong Huang Su Xuhua Zhao Guoliang Ke Xiao-Bing Zhang | 2023 | CCS Chemistry2023，5，9： | 0 |
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