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| 1 | Apoptosis and Proinflammatory Cytokine Responses of Primary Mouse Microglia and Astrocytes Induced by Human H1N1 and Avian H5N1 Influenza Viruses显示文摘Patients with an influenza virus infection can be complicated by acute encephalopathy and encephalitis. To investigate the immune reactions involved in the neurocomplication,mouse microglia and astrocytes were isolated,infected with human H1N1 and avian H5N1 influenza viruses,and examined for their immune responses. We observed homogeneously distributed viral receptors,sialic acid (SA)-α2,3-Galactose (Gal) and SA-α2,6-Gal,on microglia and astrocytes. Both viruses were replicative and productive in microglia and astrocytes. Virus-induced apoptosis and cytopathy in infected cells were observed at 24 h post-infection (p.i.). Expression of IL-1β,IL-6 and TNF-α mRNA examined at 6 h and 24 h p.i. was up-regulated,and their expression levels were considerably higher in H5N1 infection. The amounts of secreted proinflammatory IL-1β,IL-6 and TNF-α at 6 h and 24 h p.i. were also induced,with greater induction by H5N1 infection. This study is the first demonstration that both human H1N1 and avian H5N1 influenza viruses can infect mouse microglia and astrocytes and induce apoptosis,cytopathy,and proinflammatory cytokine production in them in vitro. Our results suggest that the direct cellular damage and the consequences of immunopathological injury in the CNS contribute to the influenza viral pathogenesis. | Gefei Wang Juan Zhang Weizhong Li Yun Su Yuanli Gao Heng Zhang Guimei Lin Xiaoyang Jiao Kangsheng Li | 2008 | Cellular & Molecular Immunology2008，5，2： | 28 |
| 2 | Effects of NS1 variants of H5N1 influenza virus on interferon induction, TNFa response and p53 activity显示文摘Non-structural protein 1(NS1)is an important virulence factor of the highly pathogenic H5N1 avian influenza virus.A five-amino-acid(5 aa)deletion at position 80–84 and an aspartic acid to glutamic acid substitution at position 92(D92E)are two major NS1 mutations that are highly correlated with enhanced virulence.To investigate the effect of these mutations in H5N1 virulence,three H5N1-NS1 variants were constructed:NS51(lacking 5 aa at position 80–84),NS51(I)(carrying a 5-aa insertion at position 80–84)and NS51(IM)(carrying both the 5-aa insertion and the D92E mutation).We examined the effects of these mutations on interferon(IFN)induction,tumor-necrosis factor(TNF)a response,p53 activity and apoptosis.We found that the D92E mutation eliminated NS1’s repressive effect on IFN induction,while the 5-aa deletion resulted in enhanced resistance to TNFa responses.We also observed that all three variants exhibited a similar suppressive effect on p53 transcriptional activity,although none of them significantly influenced apoptosis of host cells.Our findings shed new light on the role of NS1 in the pathogenicity of H5N1 virus. | Weizhong Li Gefei Wang Heng Zhang Gang Xin Dangui Zhang Jun Zeng Xiaoxuan Chen Yanxuan Xu Youhong Cui Kangsheng Li | 2010 | Cellular & Molecular Immunology2010，7，3： | 3 |
| 3 | Integration of high-performance spin-orbit torque MRAM devices by 200-mm-wafer manufacturing platform显示文摘We demonstrate in-plane field-free-switching spin-orbit torque(SOT)magnetic tunnel junction(MTJ)devices that are capable of low switching current density,fast speed,high reliability,and,most importantly,manufactured uniformly by the 200-mm-wafer platform.The performance of the devices is systematically studied,including their magnetic properties,switch-ing behaviors,endurance and data retention.The successful integration of SOT devices within the 200-mm-wafer manufactur-ing platform provides a feasible way to industrialize SOT MRAMs.It is expected to obtain excellent performance of the devices by further optimizing the MTJ film stacks and the corresponding fabrication processes in the future. | Hongchao Zhang Xiangyue Ma Chuanpeng Jiang Jialiang Yin Shuqin Lyu Shiyang Lu Xiantao Shang Bowen Man Cong Zhang Dandan Li Shuhui Li Wenjing Chen Hongxi Liu Gefei Wang Kaihua Cao Zhaohao Wang Weisheng Zhao | 2022 | Journal of Semiconductors2022，43，10： | 2 |
| 4 | Differential transcription-activating capability of NS1 proteins from different influenza virus subtypes expressed in yeast显示文摘Influenza A virus NS1 protein is an important regulatory factor with multiple functions and contributes greatly to viral pathogenesis.In the present study,transcription-activating potential of NS1 from different influenza A virus subtypes was examined in yeast two-hybrid system.The bait vectors contain-ing different NS1 genes,along with an empty prey vector,were transformed into yeast AH109(for growth assay on QDO plate and α-galactosidase assay),and Y187(for β-galactosidase assay).AH109 transformants with NS1 gene from H1N1,H5N1,and H9N2 viruses grew vigorously on the QDO plate and secreted high level of α-galactosidase.Also,Y187 bearing the above NS1 genes exhibited en-hanced β-galactosidase activity.Nevertheless,H3N2-NS1-transformed AH109 and Y187 yeasts did not grow on QDO plate and secrete β-galactosidase,respectively.These findings denote the remarkable variation in NS1 proteins from different influenza A virus subtypes on the transcription-stimulating capability in yeast. | LI WeiZhong,WANG GeFei,ZENG Jun,ZHANG DanGui,ZHANG Heng,CHEN XiaoXuan,CHEN YouYing & Li KangSheng Department of Microbiology and Immunology,Shantou University Medical College,Shantou 515041,China | 2009 | Science China(Life Sciences)2009，52，6： | 2 |
| 5 | Libration and end body swing stabilization of a parallel partial space elevator system显示文摘This paper studies the libration and stabilization of a parallel partial space elevator system in circular orbits. The system is made up of two paralleled partial space elevators, each of which consists of one main satellite, one end body and a climber moving along the tether between them.The libration characteristics of the elevator are studied through numerical analysis by a new dynamic model, and a novel control strategy is proposed to stabilize the swing of the end body by projecting the climber speeds only. Optimal control method is used to implement the new control strategy in the case where the climbers move in opposite direction. The simulation results validate the effectiveness of the proposed control strategy whose application will neither sacrifice the transport efficiency nor exacerbate libration significantly. | Gefei SHI Gangqiang LI Zheng H.ZHU | 2021 | Chinese Journal of Aeronautics2021，34，3： | 2 |
| 6 | In vivo antioxidant activity of polysaccharide fraction from Porphyra haitanesis in aging rats显示文摘 | Quanbin ZH Ning LI Gefei ZH | 2003 | Pharmacological Res2003，48，： | 1 |
| 7 | In vivo antioxidant activity of polysaccharide fraction from Porphyra haita-nensis in aging mice显示文摘 | Zhang Quanbin Li Ning Zhang Gefei | 2003 | Pharmacol Res2003，48，2： | 1 |
| 8 | In vivo antioxidant activity of polysaccharide fraction from Porphyru haitanensis in aging mice显示文摘 | Quanbin Zh Ning Li Gefei ZH | 2003 | Pharmacol Res2003，8，2： | 1 |
| 9 | In vivo antioxidant activity of polysaccharide fraction from Porphyra haitanesis (Rhodephyta) in aging mice显示文摘 | ZHANG Quanbin LI Ning ZHOU Gefei | 2003 | Pharmacological Research2003，48，2： | 1 |
| 10 | AS1DHRS4, a head-to-head natural antisense transcript, silences the DHRS4 gene cluster in cis and trans显示文摘 | Qi Li Zhongjing Su Xiaoyuan Xu Gefei Liu Xuhong Song Ruijian Wang Xuxia Sui Ting Liu Xiaolan Chang Dongyang Huang | 2012 | Proceedings of the National Academy of Sciences2012，，35： | 1 |
| 11 | C rohn’s disease and polymorphism of heat shock protein gene HSP 70‐2 in the C hinese population显示文摘 | Jun Chen Jianan Ren Guosheng Gu Gefei Wang Xiuwen Wu Dongsheng Yan Song Liu Jieshou Li | 2013 | J Gastroenterol Hepatol2013，，5： | 1 |
| 12 | Hydrogen bond-assisted 1,2-cis O-glycosylation under mild hydrogenolytic conditions显示文摘A hydrogen bond-assisted α-selective glycosylation reaction by using 4,6-dibenzyloxy-1,3,5-triazin-2-yl(DBT) β-glycosyl donors was developed for the efficient construction of 1,2-cis-α-glycosidic bond in natural products. This method was applied successfully to the direct synthesis of complex oligosaccharidederived glycolipids with simple protecting chemistry. Mechanistic studies using the NMR spectroscopy and DFT calculation provide a proof of concept for hydrogen bond-assisted glycosylation reaction towardsα-specific construction of O-glycosidic linkage. | Gefei Li Yanlong Luo Juan Mo Masato Noguchi Jie Jing Zhenyang Luo Shin-ichiro Shoda Xin-Shan Ye | 2023 | Chinese Chemical Letters2023，34，4： | 1 |
| 13 | In vivo antioxidant activity of polysaccharide fraction from Porphyra haitanesis (Rhodephyta) in aging mice显示文摘 | Quanbin Zhang Ning Li Gefei Zhou | 2003 | Pharmacological Research2003，48，2： | 1 |
| 14 | Recombinant human thrombopoietin improves platelet counts and reduces platelet transfusion possibility among severe sepsis patients with thrombocytopenia: A prospective study显示文摘 | Qin Wu Jianan Ren Xiuwen Wu Gefei Wang Guosheng Gu Song Liu Yin Wu Dong Hu Yunzhao Zhao Jieshou Li | 2013 | Journal of Critical Care2013，，： | 1 |
| 15 | T2 enhances in situ level of Foxp3 + regulatory cells and modulates inflammatory cytokines in Crohn’s disease显示文摘 | Guanwei Li Jianan Ren Gefei Wang Guosheng Gu Dong Hu Huajian Ren Zhiwu Hong Xiuwen Wu Song Liu Jieshou Li | 2013 | International Immunopharmacology2013，，： | 1 |
| 16 | Geographic Mapping of Crohn’s Disease and Its Relation to Affluence in Jiangsu Province, an Eastern Coastal Province of China显示文摘 | Dong Hu Jianan Ren Gefei Wang Guosheng Gu Song Liu Xiuwen Wu Jun Chen Huajian Ren Zhiwu Hong Jieshou Li Paul Henderson | 2014 | Gastroenterology Research and Practice2014，，： | 1 |
| 17 | Efficacy and Safety of Dexamethasone Ointment on Recurrent Aphthous Ulceration显示文摘 | Chuanxia Liu Zengtong Zhou Guanjian Liu Qintao Wang Jiangang Chen Ling Wang Yongmei Zhou Guangying Dong Xueyi Xu Yuechun Wang Yiqing Guo Mei Lin Lan Wu Gefei Du Changlei Wei Xin Zeng Xiaoyi Wang Junzheng Wu Bingqi Li Gang Zhou Hongmei Zhou | 2012 | The American Journal of Medicine2012，，3： | 1 |
| 18 | Rose Bengal staining in detection of oral precancerous and malignant lesions with colorimetric evaluation: a pilot study 显示文摘 | Du Gefei Li Chengzhang Chen Hanzhang | 2007 | International Journal of Cancer2007，120，9： | 1 |
| 19 | A Provably Secure General Construction for Key Exchange Protocols Using Smart Card and Password显示文摘Key exchange protocols using both smart card and password are widely used nowadays since they provide greater convenience and stronger security than protocols using only a password.Most of these protocols are often limited to simple network systems,and they may have security risks.We propose a general construction for key exchange protocols using smart card and password to avoid these flaws.The constructed protocol from the general construction has only one additional communication round than the original public encryption scheme.This construction is proven secure under random oracle model,so it can resist several common types of attacks.It is also adapted well to various networks.Compared with related protocols,the proposed key exchange protocol generated from the general construction has better secure properties and good computational efficiency in storage cost and operation time. | ZHANG Gefei FAN Dan ZHANG Yuqing LI Xiaowei | 2017 | Chinese Journal of Electronics2017，26，2： | 1 |
| 20 | In vivo antioxidant activity of polysaeeharide fraction from Porphyra haitanesis (Bhodephyta)in aging mice显示文摘 | Zhang Ouanbia Li Ning Gefei Zhou | 2003 | Pharmacological Research2003，48，2： | 1 |
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