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| 1 | Generation of rat blood vasculature and hematopoietic cells in rat-mouse chimeras by blastocyst complementation显示文摘Interspecies chimera through blastocyst complementation could be an alternative approach to create human organs in animals by using human pluripotent stem cells.A mismatch of the major histocompatibility complex of vascular endothelial cells between the human and host animal will cause graft rejection in the transplanted organs.Therefore,to achieve a transplantable organ in animals without rejection,creation of vascular endothelial cells derived from humans within the organ is necessary.In this study,to explore whether donor xeno-pluripotent stem cells can compensate for blood vasculature in host animals,we generated rat-mouse chimeras by injection of rat embryonic stem cells(rESCs)into mouse blastocysts with deficiency of Flk-1 protein,which is associated with endothelial and hematopoietic cell development.We found that rESCs could differentiate into vascular endothelial and hematopoietic cells in the rat-mouse chimeras.The whole yolk sac(YS)of Flk-1^EGFP/ECFP rat-mouse chimera was full of rat blood vasculature.Rat genes related to vascular endothelial cells,arteries,and veins,blood vessels formation process,as well as hematopoietic cells,were highly expressed in the YS.Our results suggested that rat vascular endothelial cells could undergo proliferation,migration,and self-assembly to form blood vasculature and that hematopoietic cells could differentiate into B cells,T cells,and myeloid cells in rat-mouse chimeras,which was able to rescue early embryonic lethality caused by Flk-1 deficiency in mouse. | Xiaomin Wang Hui Shi Juanjuan Zhou Qingjian Zou Quanjun Zhang Shixue Gou Pengfei Chen Lisha Mou Nana Fan Yangyang Suo Zhen Ouyang Chengdan Lai Quanmei Yan Liangxue Lai | 2020 | Journal of Genetics and Genomics2020，47，5： | 2 |
| 2 | Synthesis and characterization of well-defined cyclodextrin-centered sevenarm star poly( ε-caprolactone) s and amphiphilic star poly ( ε-caprolaetone-b-ethylene glyeol) s显示文摘 | Gou Pengfei Zhu Weipu Xu Ning | 2008 | J Polym Sci Part A: Polym Chem2008，46，： | 1 |
| 3 | Loop-mediated isothermal amplification (LAMP) method based on two species-specific primer sets for the rapid identification of Chinese Babesia bovis and B. bigemina显示文摘 | Aihong Liu Guiquan Guan Pengfei Du Huitian Gou Zhijie Liu Junlong Liu Milin Ma Jifei Yang Youquan Li Qinli Niu Qiaoyun Ren Qi Bai Hong Yin Jianxun Luo | 2012 | Parasitology International2012，，4： | 1 |
| 4 | Synthesis,extraction,and adsorption properties of calix arene-poly (ethylene-glycol) crosslinked polymer显示文摘 | ZHUWeipu GOU Pengfei ZHU Kui | 2008 | Journal of Applied Polymer Science2008，109，3： | 1 |
| 5 | Amphiphilic Seven-arm Star Triblock Copolymers with Diverse Morphologies in Aqueous Solution Induced by Crystallization and pH显示文摘 | SUN Rui WANG Ying GOU Pengfei ZUO Min LI Xiaodong ZHU Weipu SHEN Zhiquan | 2018 | Chemical Research in Chinese Universities2018，34，1： | 0 |
| 6 | A major vernalization-independent QTL for tiller angle on chromosome arm 2BL in bread wheat显示文摘Tiller angle(TA)strongly influences plant architecture and grain yield in cereals.However,the genetic basis of TA in wheat is largely unknown.We identified three TA-related quantitative trait loci(QTL).One of them was QTa.sau-2 B-769,a major QTL localized on chromosome arm 2 BL.QTa.sau-2 B-769 was detected in seven environments,explaining 18.1%–51.1%of phenotypic variance.We developed a linked Kompetitive Allele-Specific Polymerase chain reaction(KASP)marker,KASP-AX-108792274,to further validate this locus in three additional populations in multiple environments.QTa.sau-2 B-769 increased TA by up to 24.9%in these populations.There were significant and positive correlations between TA and flag leaf angle(FLANG).However,TA was not correlated with plant height or anthesis date,suggesting that expression of QTa.sau-2 B-769 is independent of vernalization.Traes CS2 B01 G583800,a gene known to be involved in leaf angle regulation,was identified as the most likely candidate gene for QTa.sau-2 B-769.These results enrich our understanding of the mechanisms regulating wheat TA at maturity and may support precise mapping and cloning of gene(s)underlying QTa.sau-2 B-769. | Jiajun Liu Jieguang Zhou Huaping Tang Yang Tu Yang Mu Lulu Gou Qiantao Jiang Yaxi Liu Guoyue Chen Jirui Wang Pengfei Qi Wei Li Yunfeng Jiang Zehong Yan Houyang Kang Yuming Wei Xiujin Lan Youliang Zheng Jian Ma | 2022 | The Crop Journal2022，10，1： | 0 |
| 7 | Novel 2D CaCl crystals with metallicity, room-temperature ferromagnetism, heterojunction, piezoelectricity-like property and monovalent calcium ions显示文摘Under ambient conditions,the only known valence state of calcium ions is+2,and the corresponding crystals with calcium ions are insulating and nonferromagnetic.Here,using cryo-electron microscopy,we report direct observation of two-dimensional(2D)CaCl crystals on reduced graphene oxide(rGO)membranes,in which the calcium ions are only monovalent(i.e.+1).Remarkably,metallic rather than insulating properties are displayed by those CaCl crystals.More interestingly,room-temperature ferromagnetism,graphene-CaCl heterojunction,coexistence of piezoelectricity-like property and metallicity,as well as the distinct hydrogen storage and release capability of the CaCl crystals in rGO membranes are experimentally demonstrated.We note that such CaCl crystals are obtained by simply incubating rGO membranes in salt solutions below the saturated concentration,under ambient conditions.Theoretical studies suggest that the formation of those abnormal crystals is attributed to the strong cation-πinteractions of the Ca cations with the aromatic rings in the graphene surfaces.The findings highlight the realistic potential applications of such abnormal CaCl material with unusual electronic properties in designing novel transistors and magnetic devices,hydrogen storage,catalyzers,high-performance conducting electrodes and sensors,with a size down to atomic scale. | Lei Zhang Guosheng Shi Bingquan Peng Pengfei Gao Liang Chen Ni Zhong Liuhua Mu Lijuan Zhang Peng Zhang Lu Gou Yimin Zhao Shanshan Liang Jie Jiang Zejun Zhang Hongtao Ren Xiaoling Lei Ruobing Yi Yinwei Qiu Yufeng Zhang Xing Liu Minghong Wu Long Yan Chungang Duan Shengli Zhang Haiping Fang | 2021 | National Science Review2021，8，7： | 0 |
| 8 | Ingenious Design of Co Al-LDH p-n Heterojunction Based on CuI as Holes Receptor for Photocatalytic Hydrogen Evolution显示文摘Reasonable design of heterojunction can greatly improve the photocatalytic hydrogen evolution activity of materials.Herein,p-n heterojunction of 2D/3D structure is constructed by the nanosheet of CoAl-LDH and rock-like CuI.The introduction of CuI can make CoAl-LDH disperse better,which brings more reaction sites for the hydrogen evolution reaction.Meanwhile,the 2D/3D structure is conducive to the construction of p-n heterojunction between the CoAl-LDH and CuI.The optical and electrochemical properties of the material indicate that the separation and transference of photon-generated carriers are promoted by the p-n heterojunction.The activity of composite catalyst(CI-10)reaches a maximum of 3.59 mmol g^(−1) h^(−1) which is 28.5 times higher than that of CuI.Furthermore,the influence of the amount of CuI and pH value on the hydrogen evolution reaction is explored.Based on the band structures of CoAl-LDH and CuI,the mechanism of photocatalytic reaction of CI-10 is proposed.The p-n heterojunction constructed with the CuI as hole receptor provides a new way to enhance the activity of photocatalytic H_(2) evolution. | Yue Cao Hongqian Gou Pengfei Zhu Zhiliang Jin | 2022 | Chinese Journal of Structural Chemistry2022，41，6： | 0 |
| 9 | Kinetics and mechanism studies on scandium calix[6]arene complex initiating ring-opening polymerization of 2,2-dimethyltrimethylene carbonate显示文摘Scandium p-tert-butylcalix[6]arene complex has been synthesized from scandium isopropoxide and p-tert-butylcalix[6]arene and used as a single component initiator for the first time. The polymerization of 2,2-dimethyltrimethylene carbonate (DTC) using this complex can proceed under mild conditions. Poly (2,2-dimethyltrimethylene carbonate) (PolyDTC) with weight-average molecular weight of 33700 and molecular weight distribution of 1.21 can be prepared. Kinetics study indicates that the polymeri- zation rate is first order with respect to both monomer and initiator concentrations, and the apparent activation energy of the polymerization is 22.7 kJ/mol. 1H NMR spectrum of the polymer reveals that the monomer ring opens via acyl-oxygen bond cleavage leading to an active center of Sc-O. | GOU PengFei ZHU WeiPu SHEN ZhiQuan | 2007 | Science China Chemistry2007，50，5： | 0 |
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