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| 1 | Targeted mutagenesis in rice using CRISPR-Cas system显示文摘 | Jin Miao Dongshu Guo Jinzhe Zhang Qingpei Huang Genji Qin Xin Zhang Jianmin Wan Hongya Gu Li-Jia Qu | 2013 | Cell Research2013，23，10： | 103 |
| 2 | Identification of a novel coronavirus causing severe pneumonia in human:a descriptive study显示文摘Background:Human infections with zoonotic coronaviruses(CoVs),including severe acute respiratory syndrome(SARS)-CoV and Middle East respiratory syndrome(MERS)-CoV,have raised great public health concern globally.Here,we report a novel batorigin CoV causing severe and fatal pneumonia in humans.Methods:We collected clinical data and bronchoalveolar lavage(BAL)specimens from five patients with severe pneumonia from Wuhan Jinyintan Hospital,Hubei province,China.Nucleic acids of the BAL were extracted and subjected to next-generation sequencing.Virus isolation was carried out,and maximum-likelihood phylogenetic trees were constructed.Results:Five patients hospitalized from December 18 to December 29,2019 presented with fever,cough,and dyspnea accompanied by complications of acute respiratory distress syndrome.Chest radiography revealed diffuse opacities and consolidation.One of these patients died.Sequence results revealed the presence of a previously unknownβ-CoV strain in all five patients,with 99.8%to 99.9%nucleotide identities among the isolates.These isolates showed 79.0%nucleotide identity with the sequence of SARS-CoV(GenBank NC_004718)and 51.8%identity with the sequence of MERS-CoV(GenBank NC_019843).The virus is phylogenetically closest to a bat SARS-like CoV(SL-ZC45,GenBank MG772933)with 87.6%to 87.7%nucleotide identity,but is in a separate clade.Moreover,these viruses have a single intact open reading frame gene 8,as a further indicator of bat-origin CoVs.However,the amino acid sequence of the tentative receptor-binding domain resembles that of SARS-CoV,indicating that these viruses might use the same receptor.Conclusion:A novel bat-borne CoV was identified that is associated with severe and fatal respiratory disease in humans. | Li-Li Ren Ye-Ming Wang Zhi-Qiang Wu Zi-Chun Xiang Li Guo Teng Xu Yong-Zhong Jiang Yan Xiong Yong-Jun Li Xing-Wang Li Hui Li Guo-Hui Fan Xiao-Ying Gu Yan Xiao Hong Gao Jiu-Yang Xu Fan Yang Xin-Ming Wang Chao Wu Lan Chen Yi-Wei Liu Bo Liu Jian Yang Xiao-Rui Wang Jie Dong Li Li Chao-Lin Huang Jian-Ping Zhao Yi Hu Zhen-Shun Cheng Un-Lin Liu Zhao-Hui Qian Chuan Qin Qi Jin Bin Cao Jian-Wei Wang | 2020 | Chinese Medical Journal2020，，9： | 99 |
| 3 | Molecular mechanism and regulation of autophagy显示文摘Autophagy 在真核细胞的房间是为长寿蛋白质和细胞质的细胞器的降级的一条主要细胞的小径。包括氨基酸饥饿和微生物的侵略,很多细胞内部 / 细胞外的刺激能在房间导致 autophagic 反应。在酵母的基因极大地预付我们参予 autophagy 和基因的分子的机制的理解的 ATG 的发现在调整 autophagic 小径包含了。许多酵母基因有哺乳动物的相当或相同的事物,建议为 autophagy 的基本机械 evolutionarily 沿着真核细胞的门被保存了。autophagy 的规定是一个很复杂的过程。许多发信号的小径包括 rapamycin (岩山) 的目标或 rapamycin (mTOR ) 的哺乳动物的目标, phosphatidylinositol 3-kinase-I (PI3K -- 我)/PKB, GTPases,钙和蛋白质合成都在调整 autophagy 起重要作用。autophagy 的分子的机制和规定在这评论被讨论。 | 2Ya-ping YANG Zhong-qin LIANG Zhen-lun GU Zheng-hong QIN | 2005 | Acta Pharmacologica Sinica2005，26，12： | 50 |
| 4 | Estimation of internal nutrient release in large shallow Lake Taihu, China显示文摘Based on field investigation of wave, sediment suspension and the changes in nutrient concentration of the water column in Lake Taihu, China, we proposed two release models to quantify nutrient release under static and dynamic conditions, respectively. Under static conditions, nutrient release from sediments to the overlying water mainly depends on chemical diffusion induced by concentration gradient, in which the nutrient release is controlled by the temperature, dissolved oxygen concentration in the sediment-water interface, oxidation-reduction potential and the concentration difference between porewater and overlying water. Under dynamic condition (or disturbed condition),both dissolved and particulate nutrients in sediments are released into the water column because of wind-induced sediment suspension. The amount of nutrient release under dynamic conditions is larger than that under the static condition. The release of dissolved nutrients, however, does not increase because the wind induced turbulence made oxidation of metallic elements such as Fe (ferric iron), Mn which are capable of precipitating soluble reactive phosphate (SRP). Under dynamic conditions, therefore, the release of total phosphorus (TP) increases dramatically but the release of SRP is close to those under static conditions. In sediments of Lake Taihu, high Fe content leads to a high ratio of Fe to P contents in sediments (Fe:P ratio). Under dynamic conditions, therefore, nutrient release is controlled by the intensity of disturbance, sediment consolidation and nutrient content in sediments.As for dissolved nutrients, especially SRP, the release is also controlled by the intensity of dynamic re-oxidation, Fe content in sediments and nutrient concentration gradient between porewater and overlying water. Based on these two release modes, the release flux in Lake Taihu has been estimated. In the static condition (i.e. laboratory experimental condition), total release of NH4+-N for whole lake is ca. 10,000 ton/a, and PO43--P is ca. 900 ton/a. In the dynamic condition, nutrient release following sediment suspension was estimated according to three different intensities of wind forcing which were defined as 'calm' (wind speed is less than 2 m/s), 'gentle' (wind speed is greater than 2 m/s and less than 6 m/s) and 'gust' (wind speed is greater than 6 m/s). The release rate in the condition of 'calm' was estimated in terms of the nutrient release in the laboratory experimental static condition; whereas the release rate in conditions of 'gentle' and 'gust' was estimated in terms of measurement during sediment resuspension conducted in flume experiments. With the observation of wind velocity and frequency in 2001, each type of wind forcing took the frequency of 12%, 82% and 6% for 'calm', 'gentle' and 'gust', respectively. The yearly release of nitrogen was 81,000 ton and phosphorus was 21,000 ton, which is about 2-6 folds of annual external loading, respectively. | QIN Boqiang, ZHU Guangwei, ZHANG Lu, LUO Liancong, GAO Guang & GU Binhe Nanjing Institute of Geography & Limnology, Chinese Academy of Sciences, Nanjing 210008, China Everglades Department, South Florida Water Management District, 3301 Gun Club Road, West Palm Beach, FL 33406, USA | 2006 | Science China Earth Sciences2006，49，z1： | 37 |
| 5 | Disruption of phytoene desaturase gene results in albino and dwarf phenotypes in Arabidopsis by impairing chlorophyll, carotenoid, and gibberellin biosynthesis显示文摘类胡萝卜素在植物和 phytoene desaturase 基因(PDS3 ) 在许多生理的过程起一个重要作用把重要的酶之一编码为类胡萝卜素生合成小径。这里,我们报导 T-DNA 插入异种 ofPDS3 基因的鉴定和分析。功能的互补证实 thepds3 异种的白化体和矮子显型源于 PDS3 基因的功能的混乱。叶绿体开发在 pds3 异种在专业版质体阶段被逮捕。进一步的分析证明高级 ofphytoene 在 pds3 异种被积累。外长的 GA (3 ) 的增加能部分救矮子显型,建议 pds3 异种的矮子显型可能由于 GAdeficiency。Microarray 和 RT-PCR 分析证明那破坏 PDS3 基因导致了涉及至少 20 条新陈代谢的小径的基因表示变化,包括在类胡萝卜素,叶绿素,和 GA 的许多基因的抑制生合成小径。我们的数据建议在 pds3 异种的积累的 phytoene 可能在某些否定反馈起一个重要作用影响多样的细胞的小径的基因表示。 | Genji Qin Hongya Gu Ligeng Ma Yiben Peng Xing Wang Deng Zhangliang Chen Li-Jia Qu | 2007 | Cell Research2007，17，5： | 39 |
| 6 | Activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice (Oryza sativa L.)显示文摘Gibberellin (GA) 2-oxidase plays a key role in the GA catabolic pathway through 2β-hydroxylation.In the present study,we isolated a CaMV 35S-enhancer activation tagged mutant,H032.This mutant exhibited a dominant dwarf and GA-deficient phenotype,with a final stature that was less than half of its wild-type counterpart.The endogenous bioactive GAs are markedly decreased in the H032 mutant,and application of bioactive GAs (GA3 or GA4) can reverse the dwarf phenotype.The integrated T-DNA was detected 12.8 kb upstream of the OsGA2ox6 in the H032 genome by TAIL-PCR.An increased level of OsGA2ox6 mRNA was detected at a high level in the H032 mutant,which might be due to the enhancer role of the CaMV 35S promoter.RNAi and ectopic expression analysis of OsGA2ox6 indicated that the dwarf trait and the decreased levels of bioactive GAs in the H032 mutant were a result of the up-regulation of the OsGA2ox6 gene.BLASTP analysis revealed that OsGA2ox6 belongs to the class III of GA 2-oxidases,which is a novel type of GA2ox that uses C20-GAs (GA12 and/or GA53) as the substrates.Interestingly,we found that a GA biosynthesis inhibitor,paclobutrazol,positively regulated the OsGA2ox6 gene.Unlike the over-expression of OsGA2ox1,which led to a high rate of seed abortion,the H032 mutant retained normal flowering and seed production.These results indicate that OsGA2ox6 mainly affects plant stature,and the dominant dwarf trait of the H032 mutant can be used as an efficient dwarf resource in rice breeding. | Jian Huang Ding Tang Yi Shen Baoxiang Qin Lilan Hong Aiqing You Ming Li Xin Wang Hengxiu Yu Minghong Gu Zhukuan Cheng | 2010 | Journal of Genetics and Genomics2010，37，1： | 36 |
| 7 | Ursolic acid induces human hepatoma cell line SMMC-7721 apoptosis via p53-dependent pathway显示文摘背景 Ursolic 酸(UA ) 是在有在 vitro 并且在 vivo 被显示出的特定的 anticancer 效果的植物王国的一个无所不在的分子。尽管 UA 能禁止肝癌症房间的增长并且导致肿瘤房间的许多类型的 apoptosis,它的 anti-hepatoma 活动的分子的机制仍然不是明确的。这研究的目的是调查禁止的效果,人的 hepatoma 房间上的 UA 的机制衬里 SMMC-7721。在有 UA 的治疗以后的方法, SMMC-7721 细胞的生长抑制被 MTT 估计试金。在他们被 UA 劝诱以后,房间被流动 cytometric 分析,染色的 Wright-Giemasa, Hoechst 33258 染色和传播电子显微镜也评估。DNA microarray 技术被用来调查暴露于 UA 40 pmol/L 的 SMMC-7721 房间的基因表示模式。房间死亡的分子的机制被即时 RT-PCR 和西方的 blotting.Results 分析 SMMC-7721 房间的增长显著地在 UA 治疗以后在一个剂量依赖者和时间依赖者举止被禁止。UA 导致了房间周期拘捕和 apoptosis。DNA microarray 分析显示 64 基因被发现到,包括下面表示的 GDF15, SOD2, ATF3,和 fos 是显著地 up-expressed, 或。西方的弄污的结果显示出 apoptotic 蛋白质 p53,当 anti-apoptotic 蛋白质 Bcl-2 是 down-regulated.Real-time RT-PCR 时, Bax 是起来调整的证实的 UA 能起来调整 GDF15, SOD2, ATF3 和下面调整的 mRNA 表情 fos 的 mRAN 表示。同时,这些效果被预告的处理部分与 p53 小径的 p53 禁止者 Pft-.Conclusion 激活堵住涉及 SMMC-7721 人的 hepatocellular 癌房间生长和 apoptosis 的正式就职的 UA 抑制。 | YU Yan-xia GU Zhen-lun YIN Jiang-lin CHOU Wen-hsien KWOK Chi-yi QIN Zheng-hong LIANG Zhong-qin | 2010 | Chinese Medical Journal2010，，14： | 32 |
| 8 | Clinical effects and complications of TIPS for portal hypertension due to cirrhosis:A single center显示文摘AIM:To determine the clinical effects and complications of transjugular intrahepatic portosystemic shunt(TIPS)for portal hypertension due to cirrhosis.METHODS:Two hundred and eighty patients with portal hypertension due to cirrhosis who underwent TIPS were retrospectively evaluated.Portal trunk pressure was measured before and after surgery.The changes in hemodynamics and the condition of the stent were assessed by ultrasound and the esophageal and fundic veins observed endoscopically.RESULTS:The success rate of TIPS was 99.3%.The portal trunk pressure was 26.8±3.6 cmH2O after surgery and 46.5±3.4 cmH2O before surgery(P<0.01).The velocity of blood flow in the portal vein increased.The internal diameters of the portal and splenic veins were reduced.The short-term hemostasis rate was100%.Esophageal varices disappeared completely in68%of patients and were obviously reduced in 32%.Varices of the stomach fundus disappeared completely in 80%and were obviously reduced in 20%of patients.Ascites disappeared in 62%,were markedly reduced in 24%,but were still apparent in 14%of patients.The total effective rate of ascites reduction was 86%.Hydrothorax completely disappeared in 100%of patients.The incidence of post-operative stent stenosis was 24%at 12 mo and 34%at 24 mo.The incidence of post-operative hepatic encephalopathy was 12%at3 mo,17%at 6 mo and 19%at 12 mo.The incidence of post-operative recurrent hemorrhage was 9%at 12mo,19%at 24 mo and 35%at 36 mo.The cumulative survival rate was 86%at 12 mo,81%at 24 mo,75%at 36 mo,57%at 48 mo and 45%at 60 mo.CONCLUSION:TIPS can effectively lower portal hypertension due to cirrhosis.It is significantly effective for hemorrhage of the digestive tract due to rupture of esophageal and fundic veins and for ascites and hydrothorax caused by portal hypertension. | Jian-Ping Qin Ming-De Jiang Wen Tang Xiao-Ling Wu Xin Yao Wei-Zheng Zeng Hui Xu Qian-Wen He Ming Gu | 2013 | World Journal of Gastroenterology2013，19，44： | 25 |
| 9 | Neuronal autophagy in cerebral ischemia显示文摘Autophagy has evolved as a conserved process for the bulk degradation and recycling of cytosolic components, such as long-lived proteins and organelles. In neurons, autophagy is important for homeostasis and protein quality control and is maintained at relatively low levels under normal conditions, while it is upregulated in response to pathophysiological conditions, such as cerebral ischemic injury. However, the role of autophagy is more complex. It depends on age or brain maturity, region, severity of insult, and the stage of ischemia. Whether autophagy plays a beneficial or a detrimental role in cerebral ischemia depends on various pathological conditions. In this review, we elucidate the role of neuronal autophagy in cerebral ischemia. | Feng Xu Jin-Hua Gu Zheng-Hong Qin | 2012 | Neuroscience Bulletin2012，28，5： | 26 |
| 10 | Multicenter case-control study of the risk factors for ulcerative colitis in China显示文摘AIM:To evaluate potential risk factors in the development of ulcerative colitis(UC) in China.METHODS:A total of 1308 patients with UC and 1308 age-matched and sex-matched controls were prospectively studied in China.The UC cases were collected from 17 hospitals in China from April 2007 to April 2010.Uniform questionnaires were designed to investigate risk factors including smoking,appendectomy,stress,socio-economic conditions,nonsteroidal antiinflammatory drugs(NSAIDs),oral contraceptives,diet,breastfeeding,infections and family sanitary conditions.Group comparisons by each factor were done using simple logistic regression analysis.Conditional logistic regression was used for multivariate analysis.RESULTS:By univariate analysis,the variables predictive of UC included feeling stress,light and heavy alcoholic drinking,spicy food,sugar consumption and infectious diarrhea,while heavy tea intake and tap water consumption were protective against UC.On multivariate analysis,the protective factor for UC was tap water consumption [odds ratios(OR) = 0.424,95%CI:0.302-0.594,P < 0.001];while the potential risk factors for UC were heavy sugar consumption(OR = 1.632,95%CI:1.156-2.305,P < 0.001),spicy food(light intake:OR = 3.329,95%CI:2.282-4.857,P < 0.001;heavy intake:OR = 3.979,95%CI:2.700-5.863,P < 0.001),and often feeling stress(OR = 1.981,95%CI:1.447-2.711,P < 0.001).Other factors,such as smoking habit,appendectomy,breastfeeding,a history of measles,rural or urban residence,education,oral contraceptives,and NSAID use have not been found to have a significant association with the development of UC in the present study.CONCLUSION:Our study showed tap water consumption was a protective factor for UC,while spicy food,heavy sugar consumption and often feeling stress were risk factors for UC in this Chinese population. | Yu-Fang Wang Qin Ou-yang Bing Xia Li-Na Liu Fang Gu Kai-Fang Zhou Qiao Mei Rui-Hua Shi Zhi-Hua Ran Xiao-Di Wang Pin-Jin Hu Kai-Chun Wu Xin-Guang Liu Ying-Lei Miao Ying Han Xiao-Ping Wu Guo-Bing He Jie Zhong Guan-Jian Liu | 2013 | World Journal of Gastroenterology2013，19，11： | 24 |
| 11 | Analysis of risk factors for polypoid lesions of gallbladder among health examinees显示文摘AIM: To investigate the prevalence and risk factors of polypoid lesions of gallbladder (PLG) among the health examinees in the Shanghai region, China. METHODS: A total of 11 816 subjects who underwent health examinations in our hospital between August 2010 and February 2011 were analyzed retrospectively. Among them, there were 7174 men and 4642 women. PLG was diagnosed by the real-time ultrasonography. Those with the body mass index (BMI) ≥ 28 were considered to be obese. Blood biochemical indices were detected with the fully automatic biochemical analyzer and hepatitis B surface antigen (HBsAg) was tested by the automated enzyme immunoassay. The correlations between the prevalence of PLG and age, sex, BMI, serum cholesterol (T-Cho), triglycerides (TG),blood sugar, HBsAg, high-density lipoprotein (HDL-C), low-density lipoprotein (LDL-C), gallstone and fatty liver were investigated. After univariate analysis of 11 variables, stepwise logistic regression analysis was performed to explore the risk factors of PLG. RESULTS: There was a significant difference in sex, T-Cho, HBsAg, HDL-C, LDL-C and fatty liver between the PLG-positive group and the PLG-negative group (332/163 vs 6842/4479, P = 0.003; 22/473 vs 295/11 026, P =0.013; 92/403 vs 993/10 328, P = 0.001; 47/448 vs 332/10 989, P = 0.001; 32/463 vs 381/10 940, P = 0.001; 83/412 vs 3260/8061, P = 0.001). No significant difference was found in the age, BMI, TG, blood sugar and gallstone between the two groups (47.3 ± 26 vs 45.1 ± 33, P = 0.173; 59/436 vs 1097/10 224, P = 0.102; 52/443 vs 982/10 339, P = 0.158; 17/478 vs 295/11 026, P = 0.26; 24/471 vs 395/10 926, P = 0.109). Logistic regression analysis showed that the sex, HBsAg and HDL-C were independent risk factors for the development of PLG in a descending order of HDL-C > HBsAg > sex. CONCLUSION: In healthy people, the male gender, positive HBsAg, and low HDL-C confer higher risks of PLG development. | Hua-Li Yang Hui-Fang Shen Yu Wang Xin-Gang Gu Jian-Min Qin Pei-Hao Yin Qi Li Lei Kong Li-Li Hou | 2012 | World Journal of Gastroenterology2012，18，23： | 24 |
| 12 | Over-expression of WOX1 Leads to Defects in Meristem Development and Polyamine Homeostasis in Arabidopsis显示文摘In plants,the meristem has to maintain a separate population of pluripotent cells that serve two main tasks,i.e.,self-maintenance and organ initiation,which are separated spatially in meristem.Prior to our study,WUS and WUS-like WOX genes had been reported as essential for the development of the SAM.In this study,the consequences of gain of WOX1 function are described.Here we report the identification of an Arabidopsis gain-of-function mutant wox1-D,in which the expression level of the WOX1(WUSCHEL HOMEOBOX 1) was elevated and subtle defects in meristem development were observed.The wox1-D mutant phenotype is dwarfed and slightly bushy,with a smaller shoot apex.The wox1-D mutant also produced small and dark green leaves,and exhibited a failure in anther dehiscence and male sterility.Molecular evidences showed that the transcription of the stem cell marker gene CLV3 was down-regulated in the meristem of wox1-D but accumulated in the other regions,i.e.,in the root-hypocotyl junction and at the sites for lateral root initiation.The fact that the organ size and cell size in leaves of wox1-D are smaller than those in wild type suggests that cell expansion is possibly affected in order to have partially retarded the development of lateral organs,possibly through alteration of CLV3 expression pattern in the meristem.An S-adenosylmethionine decarboxylase(SAMDC) protein,SAMDC1,was found able to interact with WOX1 by yeast two-hybrid and pull-down assays in vitro.HPLC analysis revealed a significant reduction of polyamine content in wox1-D.Our results suggest that WOX1 plays an important role in meristem development in Arabidopsis,possibly via regulation of SAMDC activity and polyamine homeostasis,and/or by regulating CLV3 expression. | Yanxia Zhang Renhong Wu Genji Qin Zhangliang Chen Hongya Gu Li-Jia Qu | 2011 | Journal of Integrative Plant Biology2011，53，6： | 23 |
| 13 | Human infection with a novel avian-origin influenza A (H7N9) virus: serial chest radiographic and CT findings显示文摘 | Dai Jian Zhou Xianmei Dong Danjiang Liu Yin Gu Qin Zhu Bin Wu Chao Cai Hourong | 2014 | Chinese Medical Journal2014，，12： | 22 |
| 14 | Outcomes and possible risk factors associated with axis alignment and rotational stability after implantation of the Toric implantable collamer lens for high myopic astigmatism显示文摘·AIM:To assess the visual outcomes and possible risk factors associated with axis alignment and rotational stability after implantation of Toric implantable collamer lens (TICL) for the correction of high myopic astigmatism. ·METHODS:In this prospective, nonrandomized clinical study, 54 consecutive eyes of 29 patients with high myopic astigmatism received TICL implantation. To evaluate postoperative axis deviation from the intended axis, a digital anterior segment photograph was taken. The ultrasound biomicroscopy(UBM) was used to observe footplate-position. ·RESULTS:After mean follow-up of 8.6 months, mean manifest refractive cylinder (MRC) decreased 79.3% from (-1.88±1.49)D preoperatively to (0.39±0.61)D postoperatively. MRC within 1.00D occurred in 68.5% (37/54) of eyes, whereas 48.1% (26/54) had MRC within 0.50D. Mean manifest refraction spherical equivalent (MRSE) changed from (-12.08±4.22)D preoperatively to (-0.41±0.61)D postoperatively. Uncorrected binocular vision of 20/20 or better occurred in 72.2%(39/54) of patients compared with binocular best-corrected visual acuity (BCVA) of 20/20 or better in 44.4% (24/54) preoperatively. The mean difference between intended and achieved TICL axes was (6.96±8.37)°. Footplates of TICLs were in the ciliary sulcus in 22 eyes (46.3%), below the ciliary sulcus in 32 eyes (53.7%). The angle of TICL rotation had significant correlation with the footplates-position (t =2.127; P =0.045) and the postoperative TICL vaulting (r =-0.516; P =0.000). ·CONCLUSION:The results of our study further support the safety, efficacy and predictability of TICL for the correct high myopic astigmatism. The footplate-position of TICL and vault value should be taken into consideration as two possible risks factors for TICL rotation. | Xun-Lun Sheng, De-Jun Zhu | 2012 | International Journal of Ophthalmology(English edition)2012，5，4： | 19 |
| 15 | Precise nanomedicine for intelligent therapy of cancer显示文摘Precise nanomedicine has been extensively explored for efficient cancer imaging and targeted cancer therapy, as evidenced by a few breakthroughs in their preclinical and clinical explorations. Here, we demonstrate the recent advances of intelligent cancer nanomedicine, and discuss the comprehensive understanding of their structure-function relationship for smart and efficient cancer nanomedicine including various imaging and therapeutic applications, as well as nanotoxicity. In particular, a few emerging strategies that have advanced cancer nanomedicine are also highlighted as the emerging focus such as tumor imprisonment, supramolecular chemotherapy, and DNA nanorobot. The challenge and outlook of some scientific and engineering issues are also discussed in future development. We wish to highlight these new progress of precise nanomedicine with the ultimate goal to inspire more successful explorations of intelligent nanoparticles for future clinical translations. | Huabing Chen Zhanjun Gu Hongwei An Chunying Chen Jie Chen Ran Cui Siqin Chen Weihai Chen Xuesi Chen Xiaoyuan Chen Zhuo Chen Baoquan Ding Qian Dong Qin Fan Ting Fu Dayong Hou Qiao Jiang Hengte Ke Xiqun Jiang Gang Liu Suping Li Tianyu Li Zhuang Liu Guangjun Nie Muhammad Ovais Daiwen Pang Nasha Qiu Youqing Shen Huayu Tian Chao Wang Hao Wang Ziqi Wang Huaping Xu Jiang-Fei Xu Xiangliang Yang Shuang Zhu Xianchuang Zheng Xianzheng Zhang Yanbing Zhao Weihong Tan Xi Zhang Yuliang Zhao | 2018 | Science China Chemistry2018，61，12： | 19 |
| 16 | Age-related changes of serum tartrate-resistant acid phosphatase 5b and the relationship with bone mineral density in Chinese women显示文摘瞄准:Osteoclastic 活动被尿标记的测量主要估计(例如类型的 C 终端 cross-linked telopeptides 我骨胶原,类型的 N 终端 cross-linked telopeptides 我骨胶原等等,其层次能被肾的清理经常影响。最近,浆液酒石酸盐抵抗的酸磷酸酶 5b (TRACP5b ) 被用作一个其他的浆液标记评估 osteoclastic 活动。我们在中国女人与骨头矿物质密度(BMD ) 调查了 TRACP5b 水平和它的协会的年龄相关的变化。方法:七 -- 20 79 年的 122 个中国大陆女人在学习被招募。浆液 TRACP5b 水平用免疫分析被测量评估骨头再吞的状态。骨头矿物质密度(BMD )( 在腰部的脊骨的 g/cm 2) 1 4 并且近似腿节被决斗精力 X 光检查 absorptiometry 测量。结果:浆液 TRACP5b 水平在绝经前的女人到达了底部价值 30 39,逐渐地在女人增加了 40 49,很快,在女人的玫瑰变老 50 59,并且在女人与最大的价值达到顶点 60 69 在在女人的慢落下前变老 70 79。TRACP5b 的平均水平在绝经后的女人是显著地更高的[(3.29 ± 1 .07 ) U/L ] 比在绝经前的女人([1.70 ± 0 .59 ] U/L ) 。TRACP5b 的层次相反地与根本测量的 BMD 被相关地点(P<0.001 ) 。而且, TRACP5b 的水平比有正常骨头质量(P<0.001 ) 的那些在有骨质疏松症和 osteopenia 的女人显然是更高的。结论:我们在中国大陆女人建立了浆液 TRACP5b 的参考价值,并且发现绝经后的女人比更年轻的女人有更高的 TRACP5b 集中。结果证明浆液 TRACP5b 是为骨头吸收的年龄相关的变化的评估的一个敏感、有用的参数。 | Yue-juan QIN Zhen-lin ZHANG Hao ZHANG Wei-wei HU Yu-juan LIU Yun-qiu HU Miao LI Jie-mei GU Jin-wei HE | 2008 | Acta Pharmacologica Sinica2008，29，12： | 19 |
| 17 | Arabidopsis AtBECLIN 1/AtAtg6/AtVps30 is essential for pollen germination and plant development显示文摘兼容丑恶纸巾的表面上的花粉萌芽是为植物授精的必要的步。这里,我们报导 Arabidopsis 变异的 bcl1 是由于花粉萌芽的失败不育的男性。我们证明 bcl1 异种等位基因不能被男配偶体播送,没有同型结合的 bcl1 异种被获得。花粉发育阶段的分析显示 bcl1 变化影响花粉萌芽然而并非花粉成熟。分子的分析证明花粉萌芽的失败被 AtBECLIN 1 的混乱引起。AtBECLIN 1 在成熟花粉主要被表示并且与重要相同编码蛋白质到在酵母为 autophagyand vacuolar 蛋白质排序的过程(VPS ) 要求的 Beclin1/Atg6/Vps30。我们也证明 AtBECLIN 1 为正常被要求植物开发,和那与 autophagy, VPS 和 glycosylphosphatidylinositolanchor 系统有关的基因,被 AtBECLIN 1 的缺乏影响。 | Genji Qin Zhiqiang Ma Li Zhang Shufan Xing Xianhui Hou Jie Deng Jingjing Liu Zhangliang Chen Li-Jia Qu Hongya Gu | 2007 | Cell Research2007，17，3： | 17 |
| 18 | Disruption of the 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) gene results in albino, dwarf and defects in trichome initiation and stomata closure in Arabidopsis显示文摘1-Deoxy-D-xylulose-5-phosphate reductoisomerase (DXR ) 是涉及 2-C-methyl-D-erythritol-4-phosphate (MEP ) 的重要的酶为 isoprenoid 生合成提供基本五碳的单位的小径。在植物开发和新陈代谢调查 MEP 小径的角色,我们在 dxr 异种(GK_215C01 ) 和二根 DXR 转基因的合作抑制线上执行了详细分析, OX-DXR-L2 和 OX-DXR-L7。我们发现 dxr 异种是白化体和矮子。它从来没跑,显著地减少了毛状体的数字,大多数 stomata 不能在叶子通常关门。二根合作抑制线没有毛状体生产了更黄的开花期和白化体萼片。涉及毛状体开始的基因的抄写层次被发现强烈被假装,包括 GLABRA1,透明种皮光洁 1, TRIPTYCHON 并且细长纤弱,哪个被 gibberellic 酸(气体) 调整的表示。而 abscisic 酸(骆驼毛的织物) 的外长的申请能救 stomata 闭合缺点, GA3 的外长的申请能部分救矮子显型和 dxr 的毛状体开始,建议 GA 和骆驼毛的织物的底层贡献在 dxr 异种的显型。我们进一步发现涉及 GA 和骆驼毛的织物的 biosynthetic 小径的基因并列地被调整。这些结果显示 plastidial MEP 小径的那混乱导致光合的颜料,气体和骆驼毛的织物的 biosynthetic 缺乏,并且这样发展畸形,并且从细胞质的 mevalonate 小径的流动不是足够的救缺乏,这由 plastidial MEP 小径的阻塞引起了。这些结果在控制 isoprenoids 的生合成为 MEP biosynthetic 小径揭示一个关键角色。 | Shufan Xing Jin Miao Shuang Li Genji Qin Si Tang Haoni Li Hongya Gu Li-Jia Qu | 2010 | Cell Research2010，20，6： | 17 |
| 19 | Incidence trends and age distribution of colorectal cancer by subsite in Guangzhou,2000-2011显示文摘Introduction:Colorectal cancer(CRC) is the third most common cancer in China.The incidence of CRC has been increasing in recent years.The aim of this study was to explore the incidence trends and the age distribution of CRC by subsite in Guangzhou between 2000 and 2011.Methods:A total of 22,432 incident cases of CRC between 2000 and 2011 from Guangzhou Cancer Registry were identified.Crude incidence and age-standardized rates(ASRs),using the Segi’s world standard population,were calculated for CRC and CRC subsites.The incidence trend was analyzed and the annual percentage change(APC) in incidence was calculated by using JoinPoint software.Results:The crude incidence increased significantly from 23.4/10~5 in 2000 to 37.4/10~5 in 2011 for males and from20.9/10~5 to 30.5/10~5 for females.The ASRs of CRC incidence stabilized during the period of 2000-2011 for both males and females.The ages at the onset of CRC for both males and females during 2010-2011 were significantly higher compared with those during 2000-2002(males:f = 1.95,P = 0.05;females:f = 6.03,P < 0.01).For males aged50-64 years,the CRC incidence increased by 8.50%annually(P = 0.04) during 2000-2004 and by 1.68%annually(P = 0.03) during 2005-2011.For females aged 65 years and older,the CRC incidence increased by 5.77%annually(P = 0.03) during 2000-2004.There were no significant changes for the CRC incidences in males aged 49 and younger and 65 years and older and females aged 64 years and younger during 2000-2004,or for those in all females as well as males aged 49 years and younger and 65 years and older during 2005-2011.The percentage of colon cancer in all CRCs increased significantly for both males and females between the periods of 2000-2002 and 2010-2011.The ASRs of descending colon and sigmoid colon cancer incidences increased significantly for females during 2005-2011(APC,5.51%and 1.08%,respectively,both P < 0.05).Conclusions:The crude incidence of CRC increased significantly between 2000 and 2011 because of the aging,whereas the ASRs kept stable.The percentage of colon cancer in all CRCs increased significantly.Further surveillance,research,and intervention are needed to identify the causes of these changes and to reduce the incidence and mortality of CRC. | Qin Zhou Ke Li Guo-Zhen Lin Ji-Chuan Shen Hang Dong Yu-Ting Gu Hua-Zhang Liu | 2015 | Chinese Journal of Cancer2015，34，8： | 17 |
| 20 | contrast enhanced computed tomography and reconstruction of hepatic vascular system for transjugular intrahepatic portal systemic shunt puncture path planning显示文摘AIM: To describe a method for the transjugular intrahepatic portal systemic shunt(TIPS) placement performed with the aid of contrast-enhanced computed tomography(CECT) and three-dimensional reconstructed vascular images(3D RVIs), and to assess its safety and effectiveness. METHODS: Four hundred and ninety patients were treated with TIPS between January 2005 and December 2012. All patients underwent liver CECT and reconstruction of 3D RVIs of the right hepatic vein to portal vein(PV) prior to the operation. The 3D RVIs were carefully reviewed to plan the puncture path fromthe start to target points for needle pass through the PV in the TIPS procedure. R E S U LTS :The improved TIPS procedure was successful in 483(98.6%) of the 490 patients. The number of punctures attempted was one in 294(60%) patients, 2 to 3 in 147(30%) patients, 4 to 6 in 25(5.1%) patients and more than 6 in 17(3.5%) patients. Seven patients failed. Of the 490 patients, 12 had punctures into the artery, 15 into the bile duct, eight into the gallbladder, and 18 through the liver capsule. Analysis of the portograms from the 483 successful cases indicated that the puncture points were all located distally to the PV bifurcation on anteroposterior images, while the points were located proximally to the bifurcation in the three cases with intraabdominal bleeding. The complications included three cases of bleeding, of whom one died and two needed surgery. CONCLUSION: Use of CECT and 3D RVIs to plan the puncture path for TIPS procedure is safe, simple and effective for clinical use. | Jian-Ping Qin Shan-Hong Tang Ming-De Jiang Qian-Wen He Hong-Bin Chen Xin Yao Wei-Zheng Zeng Ming Gu | 2015 | World Journal of Gastroenterology2015，21，32： | 17 |
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