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| 1 | Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface显示文摘A new cell membrane preparation, silica carrier cell membrane (CCM), has been presented, in which cell membrane was immobilized on silica which acted as carrier by the adsorption. The surface characteristics of the silica CCM have been tested by a scanning electron microscope and surface energy spectrometer. Comparison has been made of the changeable rule of enzymatic activity of rabbit red cell membrane in three states: the silica CCM, the suspension and the precipitation cell membranes, with temperature and time. The result showed that the silica CCM still keeps the same enzymatic activity as the other two states of cell membranes. The interactions between calcium antagonists and rabbit red and cardiac muscle cell membranes, and the stereoscopic interactions between dihydropridines and rabbit cerebellar cell membrane have been investigated by the chromatographic system of the silica CCM as a stationary phase. From the foregoing results, the chromatographic parameters obtained from the system would | Langchong He Guangde Yang Xindu Geng | 1999 | Chinese Science Bulletin1999，44，9： | 58 |
| 2 | The heterogeneity of islet autoantibodies and the progression of islet failure in type 1 diabetic patients显示文摘Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus. | Jin Liu Lingling Bian Li Ji Yang Chen Heng Chen Yong Gu Bingqin Ma Wei Gu Xinyu Xu Yun Shi Jian Wang Dalong Zhu Zilin Sun Jianhua Ma Hui Jin Xing Shi Heng Miao Bing Xin Yan Zhu Zhenwen Zhang Ruifang Bu Lan Xu Guangde Shi Wei Tang Wei Li Dongmei Zhou Jun Liang Xingbo Cheng Bimin Shi Jixiang Dong Ji Hu Chen Fang Shao Zhong Weinan Yu Weiping Lu Chenguang Wu Li Qian Jiancheng Yu Jialin Gao Xiaoqiang Fei Qingqing Zhang Xueqin Wang Shiwei Cui Jinluo Cheng Ning Xu Guofeng Wang Guoqing Han Chunrong Xu Yun Xie Minmin An Wei Zhang Zhixiao Wang Yun Cai Qi Fu Yu Fu Shuai Zheng Fan Yang Qingfang Hu Hao Dai Yu Jin Zheng Zhang Kuanfeng Xu Yifan Li Jie Shen Hongwen Zhou Wei He Xuqin Zheng Xiao Han Liping Yu Jinxiong She Mei Zhang Tao Yang | 2016 | Science China(Life Sciences)2016，59，9： | 5 |
| 3 | Development of the model of pancreatic β-cell membrane chromatography and its chromatographic characteristics显示文摘A new model of pancreatic β-cell membrane chromatography has been established by using a β-cell membrane stationary phase (β-CMSP) prepared by immobi-lizing the β-cell membrane onto the surface of silica carrier. The protein level and K+, Na+-ATP enzymatic activity of the β-CMSP were detected respectively. The surface characteris-tics of the β-CMSP were tested by using the scanning elec-tron microscope and surface energy spectrometer. In this model, the column (10 mm × 2 mm, I.D.) packed with β-CMSP, 25 mmo1/L ammonium sulfate buffer solution (pH 7.4) as mobile phase with the flow rate of 0.2 mL/min at 37 ± 0.5℃ were used in the following studies. The retention char-acteristics of the sulfonylureas (gliquidone, glibenclamide, gliclazide and glipizide) were investigated under the chro-matographic conditions above. The affinities of the sulfony-lureas on β-cell membrane and receptor will be expressed by using the logk′ values (the logarithm of capacity factor of a solute) in the model. The correlation of the affinity with the pharmacological effect of the sulfonylureas was analyzed also. | YANG Guangde HE Langchong BIAN Xiaoli ZHAO Liang | 2005 | Chinese Science Bulletin2005，50，21： | 2 |
| 4 | Study of atmospheric pressure microwave plasma spectrometry显示文摘 | Jin Qinhan Yang Guangde Yu Aimin | 1985 | Acta Scientiarum Naturalium Universitatis Jilinensis1985，，1： | 1 |
| 5 | Imbalanced Intrahepatic Cytokine Expression of Interferon-γ, Tumor Necrosis Factor-α, and Interleukin-10 in Patients With Acute-on-Chronic Liver Failure Associated With Hepatitis B Virus Infection显示文摘 | Zhengsheng Zou Baosen Li Dongping Xu Zheng Zhang Jing-Min Zhao Guangde Zhou Yanling Sun Lei Huang Junliang Fu Yongping Yang Lei Jin Wei Zhang Jun Zhao Ying Sun Shaojie Xin Fu-Sheng Wang | 2009 | Journal of Clinical Gastroenterology2009，，2： | 1 |
| 6 | Comparison of Acoustic Radiation Force Impulse Imaging and Transient Elastography for Non-invasive Assessment of Liver Fibrosis in Patients with Chronic Hepatitis B显示文摘 | Dakun Zhang Min Chen Ruifang Wang Yang Liu Dedong Zhang Liping Liu Guangde Zhou | 2014 | Ultrasound in Medicine & Biology2014，，： | 1 |
| 7 | Cell Membrane Chromatography Correlated with Functional Assay for Ligand–β-Adrenergic Receptor Affinities显示文摘 | Aiguo Zeng Bingxiang Yuan Fan Zhu Limei Zhao Langchong He Guangde Yang | 2009 | Chromatographia (-)2009，，11： | 1 |
| 8 | Pathological study of 130 cases of nonalcoholic fatty liver disease based on NASH-CRN system显示文摘To summarize the pathological features of non-alcoholic liver disease(NAFLD)in China based on a histo-logical scoring system for NAFLD designed by the Pathology Committee of NASH Clinical Research Network(NASH-CRN),the specimens of liver needle biopsies from 130 patients with NAFLD were histopathologically analyzed by haematoxylin eosin,reticular fiber and Masson trichrome stain.Immunohistochemistry staining was used to exclude non-NAFLD cases combined with clinical data.Hepatic steatosis,lobular inflammation,hepatocytic ballooning and fibrosis were presented widespread in NAFLD liver tissues.Furthermore,macrovesicular steatosis predominantly located in acinar zone 3 was the main histologic feature of NAFLD and lobular inflammation was usually presented mildly.Hepatocyte ballooning was observed in 94.6%of all 130 cases.Mild perisinusoidal fibrosis and periportal fibrosis were often observed in stage 1 cases.According to the stati-stic analysis,hepatic steatosis was positively correlated with lobular inflammation,hepatocytic ballooning and fibrosis(r=0.587,0.488,0.374,respectively,all P value<0.01).The number of microgranulomas,lipogranulomas and apo-ptotic bodies increased following severity of steatosis,lobular inflammation and fibrosis.Meanwhile,the number of mega-mitochondria and glycogen nuclei was paralleveled to the degree of hepatocytic ballooning(P value all<0.01).We suggest that the role of portal inflammation should be empha-sized besides hepatic steatosis,lobular inflammation,hepa-tocyte ballooning and fibrosis in diagnosis and evaluation of NAFLD.It needs to be further verified whether microgranu-lomas,lipogranulomas and apoptosis bodies could be used as histopathological markers of development of NAFLD.The number of megamitochondria is more frequently be found in NAFLD,while in alcoholic liver diseases was Mallory bodies. | ZHOU Guangde ZHAO Jingmin DING Xiaohui PAN Deng SUN Yanling YANG Jianfa ZHAO Yulai | 2007 | Frontiers of Medicine2007，1，4： | 0 |
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