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| 1 | Effects of Percutaneous Coronary Intervention on Serum Angiopoietin-2 in Patients with Coronary Heart Disease显示文摘 | Zhi-Yu Zeng Chun Gui Lang Li Xiao-Min Wei | 2016 | Chinese Medical Journal2016，，6： | 22 |
| 2 | Responses of root growth of Alhagi sparsifolia Shap. (Fabaceae) to different simulated groundwater depths in the southern fringe of the Taklimakan Desert, China显示文摘Alhagi sparsifolia Shap. (Fabaceae) is a spiny, perennial herb. The species grows in the salinized, arid regions in North China. This study investigated the response characteristics of the root growth and the distribution of one-year-old A. sparsifolia seedlings to different groundwater depths in controlled plots. The ecological adaptability of the root systems of A. sparsifolia seedlings was examined using the artificial digging method. Results showed that: (1) A. sparsifolia seedlings adapted to an increase in groundwater depth mainly through increasing the penetration depth and growth rate of vertical roots. The vertical roots grew rapidly when soil moisture content reached 3%-9%, but slowly when soil moisture content was 13%-20%. The vertical roots stopped growing when soil moisture content reached 30% (the critical soil moisture point). (2) The morphological plasticity of roots is an important strategy used by A. sparsifolia seedlings to obtain water and adapt to dry soil conditions. When the groundwater table was shallow, horizontal roots quickly expanded and tillering increased in order to compete for light resources, whereas when the groundwater table was deeper, vertical roots developed quickly to exploit space in the deeper soil layers. (3) The decrease in groundwater depth was probably responsible for the root distribution in the shallow soil layers. Root biomass and surface area both decreased with soil depth. One strategy of A. sparsifolia seedlings in dealing with the increase in groundwater depth is to increase root biomass in the deep soil layers. The relationship between the root growth/distribution of A. sparsifolia and the depth of groundwater table can be used as guidance for harvesting A. sparsifolia biomass and managing water resources for forage grasses. It is also of ecological significance as it reveals how desert plants adapt to arid environments. | FanJiang ZENG Cong SONG HaiFeng GUO Bo LIU WeiCheng LUO DongWei GUI Stefan ARNDT DaLi GUO | 2013 | Journal of Arid Land2013，5，2： | 13 |
| 3 | The Effect of PCDH15 Gene Variations on the Risk of Noise-induced Hearing Loss in a Chinese Population显示文摘导致噪音的听觉损失(NIHL ) 是在环境、基因的因素之间的相互作用引起的复杂疾病。这研究调查了在 protocadherin 的基因可变性是否联系了 15 (PCDH15 ) 在一张中国人口位于增加的危险性下面到 NIHL 的发展。结果证明与 rs11004085 的 TT 遗传型相比, CT/CC 遗传型与 NIHL 的增加的风险被联系[调整机会比率(或)= 2.64;95% 信心间隔(CI ) :1.14-6.11, P = 0.024 ] 。另外,在 rs11004085 和 rs978842 之间的重要相互作用基因变化和噪音暴露在高级暴露组被观察(P < 0.05 ) 。而且,当与噪音暴露的高水平结合了时,风险 haplotype TAGCC 被观察(P < 0.05 ) 。因此,我们的学习证实在 PCDH15 的基因变化在人修改危险性到 NIHL 开发。 | XU Xiang Rong WANG Jing Jing YANG Qiu Yue JIAO Jie HE Li Hua YU Shan Fa GU Gui Zhen CHEN Guo Shun ZHOU Wen Hui WU Hui LI Yan Hong ZHANG Huan Ling ZHANG Zeng Rui JIN Xian Ning | 2017 | Biomedical and Environmental Sciences2017，30，2： | 9 |
| 4 | First-principles study of structure,mechanical and optical properties of La-and Sc-doped Y2O3显示文摘The electronic,mechanical and optical properties of La-and Sc-doped Y2O3 were investigated using firstprinciples calculations.Two doping sites of Sc and La in Y2O3 were modeled.The calculated values of the energy of formation show that the most energetically favorable site for a La atom in Y2O3 is a d-site Y atom,while for Sc a b-site Y atom is the more stable position.The calculated band gap shows a slight decrease with increasing La or Sc concentration.The calculated results for the mechanical and optical properties of Y(2-x)RxO3(R=Sc or La,0 | Xian Zhang Sen Gao Wenhua Gui Qingfeng Zeng | 2019 | Journal of Rare Earths2019，37，8： | 8 |
| 5 | An-Luo-Hua-Xian Pill Improves the Regression of Liver Fibrosis in Chronic Hepatitis B Patients Treated with Entecavir显示文摘Background and Aims:Chronic hepatitis B(CHB)can cause liver fibrosis and lead to cirrhosis and cancer.As the effectiveness of antiviral therapy to reverse liver fibrosis is limited,We aimed to evaluate the effect of An-Luo-Hua-Xian pill(ALHX)on fibrosis regression in CHB patients treated with entecavir(ETV).Methods:Treatment-naïve patients with CHB were randomly treated with ETV alone or combined with ALHX(ETV+ALHX)between October 1,2013 and December 31,2020.Demographic,laboratory,and liver histology data before and after 78 weeks of treatment were collected.The Ishak fibrosis score(F)was used and fibrosis regression required a decrease in F of≥1 after treatment.Results:A total of 780 patients were enrolled,and 394 with a second liver biopsy after treatment were included in the per-protocol population,132 in ETV group and 262 in ETV+ALHX group.After 78 weeks of treatment,the fibrosis regression rate in the ETV+ALHX group was significantly higher than that of the ETV group at baseline F≥3 patients:124/211(58.8%)vs.45/98(45.9%),p=0.035.The percentage of patients with a decreased liver stiffness measurement(LSM)was higher in the ETV+ALHX group:156/211(73.9%)vs.62/98(63.%),p=0.056.Logistic regression analysis showed that ETV combined with ALHX was associated with fibrosis regression[odds ratio(OR)=1.94,p=0.018],and a family history of hepatocellular carcinoma was on the contrary.(OR=0.41,p=0.031).Conclusions:ETV combined with ALHX increased liver fibrosis regression in CHB patients. | Yi-Qi Liu Chi Zhang Jia-Wen Li Li-Hua Cao Zhan-Qing Zhang Wei-Feng Zhao Qing-Hua Shang Da-Zhi Zhang An-Lin Ma Qing Xie Hong-Lian Gui Guo Zhang Ying-Xia Liu Jia Shang Shi-Bin Xie Jun Li Xu-Qing Zhang Zhi-Qiang Zou Yu-Ping Chen Zong Zhang Ming-Xiang Zhang Jun Cheng Fu-Chun Zhang Li-Hua Huang Jia-Bin Li Qing-Hua Meng Hai-Bin Yu Yu-Qiang Mi Yan-Zhong Peng Zhi-Jin Wang Li-Ming Chen Fan-Ping Meng Wan-Hua Ren Lang Bai Yi-Lan Zeng Rong Fan Xian-Zhi Lou Wei-Feng Liang Hui Liu Hui Zhuang Hong Zhao Gui-Qiang Wang | 2023 | Journal of Clinical and Translational Hepatology2023，11，2： | 7 |
| 6 | Multiple constraints QoS multicast routing optimization algorithm in MANET based on GA显示文摘Usually multiple quality of service (QoS) guarantees are required in most multicast applications. This paper presents a multiple con-straints algorithm for multicast traffic engineering in mobile ad hoc networks (MANET). The proposed algorithm is a new version of multiple constraints QoS multicast routing optimization algorithm in MANET based on genetic algorithm (MQMGA). The proposed MQMGA can optimize the maximum link utilization, the cost of the multicast tree, the selection of the long-life path, the average delay and the maximum end-to-end delay. Experimental result shows that the approach is efficient, has promising performance in multicast traffic engineering and for evaluating the route stability in dynamic mobile networks. | Baolin Sun Shangchao Pi Chao Gui Yue Zeng Bing Yan Wenxiang Wang Qianqing Qin | 2008 | Progress in Natural Science:Materials International2008，18，3： | 6 |
| 7 | A draft sequence of the rice (Oryza sativa ssp. indica) genome显示文摘The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a 'whole genome shotgun' approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package | YU Jun, HU Songnian, WANG Jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, LIU Jing,SUN Jiandong , TANG Jiabin, CHEN Yanjiong,HUANG Xiaobing, LIN Wei, YE Chen, TONG Wei,CONG Lijuan, GENG Jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Long,LIU Jianping, Ql Qiuhui, LIU Jinsong, LI Li,WANG Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI Peng,LI Xianran, WANG Hao, XU Xin, ZHAI Wenxue,XU Zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO Lin, CHEN Xuewei, HE Jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, CHEN Wei ,WANG Xudong, ZHANG Yong, HU Jianfei,WANG Jing, LIU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, ZHOU Chengshu, ZHU Zhen,CHEN Runsheng, HAO Bailin,ZHENG Weimou, CHEN Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, WANG Jian, ZHU Lihuang,YUAN Longping& YANG HuanmingBeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, China Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China National Hybrid Rice R & D Center, Changsha 410125, China Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China College of Life Sciences, Peking University, Beijing 100871, China Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 1Q0080, China Digital China Ltd., Beijing 100080, China Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China Medical College, Xi’an Jiaotong University, Xi’an 710061, ChinaThese authors contributed equally to this work.Corresponding author.Corresponden | 2001 | Chinese Science Bulletin2001，46，23： | 6 |
| 8 | Comparison of diagnostic efficacy between transrectal and transperineal prostate biopsy:a propensity score-matched study显示文摘This study compared the diagnostic efficacy of transrectal ultrasound(TRUS)-guided prostate biopsy(TRBx)and transperineal prostate biopsy(TPBx)in patients with suspected prostate cancer(PCa).We enrolled 2962 men who underwent transrectal(n=1216)or transperineal(n=1746)systematic 12-core prostate biopsy.Clinical data including age,prostate-specific antigen(PSA)level,and prostate volume(PV)were recorded.To minimize confounding,we performed propensity score-matching analysis?We measured and compared PCa detection rates between TRBx and TPBx,which were stratified by clinical characteristics and Gleason scores.The effects of clinical characteristics on PCa detection rate were assessed by logistic regression.For all patients,TPBx detected a higher proportion of clinically significant PCa(P<0.001).Logistic regression analyses illustrated that PV had a smaller impact on PCa detection rate of TPBx compared with TRBx.Propensity score-matching analysis showed that the detection rates in TRBx were higher than those in TPBx for patients aged≥80 years(80.4%vs 56.5%,P=0.004)and with PSA level 20.1-100.0 ng ml^-1(80.8%vs 69.1%,P=0.040).In conclusion,TPBx was associated with a higher detection rate of clinically significant PCa than TRBx was;however,because of the high detection rate at certain ages and PSA levels,biopsy approaches should be optimized according to patents'clinical characteristics. | Chen-Yi Jiang Peng-Fei Shen Cheng Wang Hao-Jun Gui Yuan Ruan Hao Zeng Shu-Jie Xia Qiang Wei Fu-Jun Zhao | 2019 | Asian Journal of Andrology2019，21，6： | 6 |
| 9 | Root characteristics of Alhagi sparsifolia seedlings in response to water supplement in an arid region,northwestern China显示文摘The effect of variation in water supply on woody seedling growth in arid environments remain poorly known.The subshrub Alhagi sparsifolia Shap.(Leguminosae),distributed in the southern fringe of the Taklimakan Desert,Xinjiang,northwestern China,has evolved deep roots and is exclusively dependent on groundwater,and performs a crucial role for the local ecological safety.In the Cele oasis,we studied the responses of A.sparsifolia seedling roots to water supplement at 10 and 14 weeks under three irrigation treatments(none water supply of 0 m3/m2(NW),middle water supply of 0.1 m3/m2(MW),and high water supply of 0.2 m3/m2(HW)).The results showed that the variations of soil water content(SWC) significantly influenced the root growth of A.sparsifolia seedlings.The leaf area,basal diameter and crown diameter were significantly higher in the HW treatment than in the other treatments.The biomass,root surface area(RSA),root depth and relative growth rate(RGR) of A.sparsifolia roots were all significantly higher in the NW treatment than in the HW and MW treatments at 10 weeks.However,these root parameters were significantly lower in the NW treatment than in the other treatments at 14 weeks.When SWC continued to decline as the experiment went on(until less than 8% gravimetric SWC),the seedlings still showed drought tolerance through morphological and physiological responses,but root growth suffered serious water stress compared to better water supply treatments.According to our study,keeping a minimum gravimetric SWC of 8% might be important for the growth and establishment of A.sparsifolia during the early growth stage.These results will not only enrich our knowledge of the responses of woody seedlings to various water availabilities,but also provide a new insight to successfully establish and manage A.sparsifolia in arid environments,further supporting the sustainable development of oases. | DongWei GUI FanJiang ZENG Zhen LIU Bo ZHANG | 2013 | Journal of Arid Land2013，5，4： | 5 |
| 10 | Utility of Droplet Digital PCR Assay for Quantitative Detection of Norovirus in Shellfish, from Production to Consumption in Guangxi, China显示文摘Objective Shellfish are recognized as important vehicles of norovirus-associated gastroenteritis. The present study aimed to monitor norovirus contamination in oysters along the farm-to-fork continuum in Guangxi, a major oyster production area in Southwestern China. Methods Oyster samples were collected monthly from farms, markets, and restaurants, from January to December 2016. Norovirus was detected and quantified by one-step reverse transcription-droplet digital polymerase chain reaction(RT-ddPCR). Results A total of 480 oyster samples were collected and tested for norovirus genogroups I and II. Norovirus was detected in 20.7% of samples, with genogroup II predominating. No significant difference was observed in norovirus prevalence among different sampling sites. The norovirus levels varied widely, with a geometric mean of 19,300 copies/g in digestive glands. Both norovirus prevalence and viral loads showed obvious seasonality, with a strong winter bias. Conclusion This study provides a systematic analysis of norovirus contamination ‘from the farm to the fork' in Guangxi. RT-ddPCR can be a useful tool for detection and quantification of low amounts of norovirus in the presence of inhibitors found particularly in foodstuffs. This approach will contribute to the development of strategies for controlling and reducing the risk of human illness resulting from shellfish consumption. | TAN Dong Mei LYU Su Ling LIU Wei ZENG Xian Ying LAN Lan QU Cong ZHUGE Shi Yang ZHONG Yan Xu XIE Yi Hong LI Xiu Gui | 2018 | Biomedical and Environmental Sciences2018，31，10： | 4 |
| 11 | Imazamox microbial degradation by common clinical bacteria: Acinetobacter baumannii IB5 isolated from black soil in China shows high potency显示文摘Herbicidal residues of imazamox are hazardous to some sensitive rotational aftercrops.The aim of the study was to isolate and identify a microbial strain capable of degrading imazamox.The strain IB5,capable of efficiently degrading imazamox,was isolated from an imazamox-contaminated soybean field in Heilongjiang Province,China.It was found to degrade 98.61%of 400 mg L^(-1) imazamox within 48 h by high-performance liquid chromatograph.Through morphological,physiological and biochemical characterization,and the 16 S rDNA sequencing,the strain was identified as Acinetobacter baumannii.An optimal degradation condition was obtained and verified:400 mg L^(-1) imazamox,0.1%(volume ratio) initial inoculum,37℃and pH 7.0.Four main products were captured in the liquid chromatograms and mass spectra,and a pathway for imazamox degradation by IB5 was proposed.This work provides a new suitable candidate for imazamox biodegradation and theoretical evidence for imazamox residue bioremediation.A.baumannii is a common clinical bacteria,but its imazamox-degrading feature has not been reported previously. | LIU Chun-guang YANG Xin LAI Yang LU Hong-gang ZENG Wei-min GENG Gui YANG Feng-shan | 2016 | Journal of Integrative Agriculture2016，15，8： | 4 |
| 12 | A new prenylated arylbenzofuran derivative from Morus alba L.显示文摘2-[3,5-Di-O-β-D-glucosyl-4-(3-methylbut-2-enyl)phenyl]benzofuran-6-ol,a new prenylated arylbenzofuran derivative was isolated from Morus alba L.Its structure was elucidated by various spectroscopic methods including MS,~1H NMR,^(13)C NMR,DEPT,~1H-~1HCOSY,HMQC and HMBC. | Tian, Hai Ying He, Xi Zeng, Guang Yao Tan, Jian Bin Shuang, Fu Liu, Guo Ru Tan, Gui Shan Zhou, Ying Jun | 2010 | Chinese Chemical Letters2010，21，3： | 3 |
| 13 | Modified Glasgow Prognostic Score, and Neutrophil/lymphocyte and Platelet/lymphocyte Ratios in Different Stages of Silicosis显示文摘Until now, although comprehensive management strategies have improved treatment, there are no treatments to alleviate symptoms and slow disease progression[1]. In the past few decades, there has been increasing evidence that inflammation plays a very important role in silicosis. Injury-induced inflammation is an effective strategy to remove harmful stimuli and initiate a healing process. However, it might be harmful to the organism and result in a permanent disease state if the inflammation is prolonged[2]. | HE La Gu WU Peng Fei PENG Yan Hua FENG Juan ZHONG Dai Ming ZHANG Gui Hua ZENG Lei ZHONG Yu Min WANG Xiao Ye YUAN Hua Min | 2019 | Biomedical and Environmental Sciences2019，32，5： | 3 |
| 14 | A Data and Knowledge Collaboration Strategy for Decision-Making on the Amount of Aluminum Fluoride Addition Based on Augmented Fuzzy Cognitive Maps显示文摘In the aluminum reduction process, aluminum uoride (AlF3) is added to lower the liquidus temperature of the electrolyte and increase the electrolytic ef ciency. Making the decision on the amount of AlF3 addi- tion (referred to in this work as MDAAA) is a complex and knowledge-based task that must take into con- sideration a variety of interrelated functions;in practice, this decision-making step is performed manually. Due to technician subjectivity and the complexity of the aluminum reduction cell, it is dif cult to guarantee the accuracy of MDAAA based on knowledge-driven or data-driven methods alone. Existing strategies for MDAAA have dif culty covering these complex causalities. In this work, a data and knowl- edge collaboration strategy for MDAAA based on augmented fuzzy cognitive maps (FCMs) is proposed. In the proposed strategy, the fuzzy rules are extracted by extended fuzzy k-means (EFKM) and fuzzy deci- sion trees, which are used to amend the initial structure provided by experts. The state transition algo- rithm (STA) is introduced to detect weight matrices that lead the FCMs to desired steady states. This study then experimentally compares the proposed strategy with some existing research. The results of the comparison show that the speed of FCMs convergence into a stable region based on the STA using the proposed strategy is faster than when using the differential Hebbian learning (DHL), particle swarm optimization (PSO), or genetic algorithm (GA) strategies. In addition, the accuracy of MDAAA based on the proposed method is better than those based on other methods. Accordingly, this paper provides a feasible and effective strategy for MDAAA. | Weichao Yue Weihua Gui Xiaofang Chen Zhaohui Zeng Yongfang Xie | 2019 | Engineering2019，5，6： | 3 |
| 15 | Spirulina platensis aqueous extracts ameliorate colonic mucosal damage and modulate gut microbiota disorder in mice with ulcerative colitis by inhibiting inflammation and oxidative stress显示文摘Ulcerative colitis(UC)is a chronic and recurrent inflammatory bowel disease(IBD)that has become a major gastroenterologic problem during recent decades.Numerous complicating factors are involved in UC development such as oxidative stress,inflammation,and microbiota disorder.These factors exacerbate damage to the intestinal mucosal barrier.Spirulina platensis is a commercial alga with various biological activity that is widely used as a functional ingredient in food and beverage products.However,there have been few studies on the treatment of UC using S.platensis aqueous extracts(SP),and the underlying mechanism of action of SP against UC has not yet been elucidated.Herein,we aimed to investigate the modulatory effect of SP on microbiota disorders in UC mice and clarify the underlying mechanisms by which SP alleviates damage to the intestinal mucosal barrier.Dextran sulfate sodium(DSS)was used to establish a normal human colonic epithelial cell(NCM460)injury model and UC animal model.The mitochondrial membrane potential assay 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and staining with Annexin V-fluorescein isothiocyanate(FITC)/propidium iodide(PI)and Hoechst 33258 were carried out to determine the effects of SP on the NCM460 cell injury model.Moreover,hematoxylin and eosin(H&E)staining,transmission electron microscopy(TEM),enzyme-linked immunosorbent assay(ELISA),quantitative real-time polymerase chain reaction(qPCR),western blot,and 16S ribosomal DNA(rDNA)sequencing were used to explore the effects and underlying mechanisms of action of SP on UC in C57BL/6 mice.In vitro studies showed that SP alleviated DSS-induced NCM460 cell injury.SP also significantly reduced the excessive generation of intracellular reactive oxygen species(ROS)and prevented mitochondrial membrane potential reduction after DSS challenge.In vivo studies indicated that SP administration could alleviate the severity of DSS-induced colonic mucosal damage compared with the control group.Inhibition of inflammation and oxidative stress was associated with increases in the activity of antioxidant enzymes and the expression of tight junction proteins(TJs)post-SP treatment.SP improved gut microbiota disorder mainly by increasing antioxidant enzyme activity and the expression of TJs in the colon.Our findings demonstrate that the protective effect of SP against UC is based on its inhibition of pro-inflammatory cytokine overproduction,inhibition of DSS-induced ROS production,and enhanced expression of antioxidant enzymes and TJs in the colonic mucosal barrier. | Jian WANG Liqian SU Lun ZHANG Jiali ZENG Qingru CHEN Rui DENG Ziyan WANG Weidong KUANG Xiaobao JIN Shuiqing GUI Yinghua XU Xuemei LU | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，6： | 3 |
| 16 | Transcriptomics-based identification and characterization of 11 CYP450 genes of Panax ginseng responsive to MeJA显示文摘Cytochromes P450 (CYP450s),a superfamily of mono-oxygenases,are essential to generate highly functionalized secondary metabolites in plants and contribute to the diversification of specialized triterpenoid biosynthesis in eudicots.However,screening and identifying the exact CYP450 genes in ginsenoside biosynthesis is extremely challenging due to existence of large quantities of members in CYP450 superfamily.Therefore,to screen the CYP450 genes involved in ginsenoside bio- synthesis,transcriptome dataset of Panax ginseng was created in our previous work using the technique of the next-generation sequencing.On the basis of bioinformatics analysis,16 putative CYP450 genes with significant differential expression were screened from the dataset and submit- ted to GenBank,in which 11 of them have been cloned.Methyl jasmonate (MeJA)was used as an elicitor to analyze the expression profiles of candidate CYP450 genes by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR).The results of qRT-PCR analysis revealed that the expression of some CYP450 genes were strongly induced by MeJA and showed different transcription levels at different treatment time points.Homology analysis indicated that each putative CYP450 protein of P.ginseng has a conserved domain consisting of E-E-R-F-P-R-G. The CYP450 genes were screened and cloned here to enrich the resources of CYP450 genes,and the results of bioinformatics analysis provided a foundation to further identify the function of CYP450s involved in ginsenoside biosynthesis.Furthermore,this study facilitated the construction of microbial cell factories for increasing the production of ginsenosides by means of metabolic engineering. | Xu Zeng Tiao Luo Jijia Li Gui Li Donghua Zhou Tuo Liu Xian Zou Aparna Pandey Zhiyong Luo | 2018 | Acta Biochimica et Biophysica Sinica2018，50，11： | 2 |
| 17 | Cis-2-dodecenoic Acid Mediates Its Synergistic Effect with Triazoles by Interfering with Efflux Pumps in Fluconazole-resistant Candida albicans显示文摘Objective To evaluate the synergy of the Burkholderia signaling molecule cis-2-dodecenoic acid(BDSF) and fluconazole(FLU) or itraconazole(ITRA) against two azole-resistant C. albicans clinical isolates in vitro and in vivo. Methods Minimum inhibitory concentrations(MICs) of antibiotics against two azole-resistant C. albicans were measured by the checkerboard technique, E-test, and time-kill assay. In vivo antifungal synergy testing was performed on mice. Analysis of the relative gene expression levels of the strains was conducted by quantitative reverse-transcription polymerase chain reaction(qR T-PCR). Results BDSF showed highly synergistic effects in combination with FLU or ITRA with a fractional inhibitory concentration index of ≤ 0.08. BDSF was not cytotoxic to normal human foreskin fibroblast cells at concentrations of up to 300 μg/mL. The qR T-PCR results showed that the combination of BDSF and FLU/ITRA significantly inhibits the expression of the efflux pump genes CDR1 and MDR1 via suppression of the transcription factors TAC1 and MRR1, respectively, when compared with FLU or ITRA alone. No dramatic difference in the mR NA expression levels of ERG1, ERG11, and UPC2 was found, which indicates that the drug combinations do not significantly interfere with UPC2-mediated ergosterol levels. In vivo experiments revealed that combination therapy can be an effective therapeutic approach to treat candidiasis. Conclusion The synergistic effects of BDSF and azoles may be useful as an alternative approach to control azole-resistant Candida infections. | YANG Dong Liang HU Yan Ling YIN Zi Xin ZENG Gui Sheng LI Dan ZHANG Yu Qian XU Zhen Hua GUAN Xiao Ming WENG Li Xing WANG Lian Hui | 2019 | Biomedical and Environmental Sciences2019，32，3： | 2 |
| 18 | Water use efficiency and yield responses of cotton to field capacity-based deficit irrigation in an extremely arid area of China显示文摘The objectives of present investigation were to test the effects on water use efficiency(WUE)and cotton yield of implementing a range of deficit irrigation regimes triggered at specific fractions of root zone soil moisture,field capacity(θfc)and different crop phenological stages.The study was conducted on southern oasis of the Taklamakan desert,China.The cotton crop’s WUE was quantified,as were leaf photosynthesis and yield.From a photosynthetic perspective,deficit irrigation resulted in 16.8%,10.3%and 2.2%increases in leaf WUE underθfc-based regulated deficit irrigation(T1,T2,and T3),compared to the control,respectively.Cotton yield and its components were significantly affected by irrigation depths(p≤0.05).A relatively high seed yield(0.65 kg/m3)and the highest WUE were achieved,under T3(70%θfc at seedling stage,60%θfc at squaring,50%θfc at full-bloom,70%θfc at boll,70%θfc at boll cracking stage),showing it to be the most effective and productive irrigation schedule tested.As the application ofθfc-based deficit irrigation in surface-irrigated cotton fields showed great potential in saving water,maintaining a high WUE,and improving cotton seed yield,a management strategy consisting or irrigation thresholds of 70%θfc in the root zone at the seedling,boll and boll cracking stages,and of 60%θfc at the squaring stage,and 50%θfc at the full-bloom stage,would be recommended for this extremely arid region. | Haifeng Li Zhiming Qi Dongwei Gui Fanjiang Zeng | 2019 | International Journal of Agricultural and Biological Engineering2019，12，6： | 2 |
| 19 | Predictive efficacy of the 2014 International Society of Urological Pathology Gleason grading system in initially diagnosed metastatic prostate cancer显示文摘我们比较了在开始与变形前列腺癌症(PCa ) 诊断的 568 个病人分级系统的 2014 和 2005 格利森的预兆的能力。结果包括了阉割抵抗的前列腺的持续时间没有癌症的幸存(CFS ) 和全面幸存(OS ) 。Univariate 分析和木头等级测试被用来识别预后指示物并且在格利森在 CFS 和 OS 估计 univariable 差别分数(GS ) 组。考克斯在接收装置操作员特征方法的曲线下面的比例的危险和区域被用来评估分级系统的 2005 和 2014 ISUP 的预兆的功效。Univariate 分析证明 2005 和 2014 个分级的系统是为 CFS 和 OS 的预言者;两个系统能与 GS 6, GS 7,和 GS 8-10 区分病人的临床的结果。用 2014 个标准,在耐心的幸存的统计差别都没在 GS 3 + 之间被观察 4 并且 GS 4 + 3 或 GS 8 和 GS 9-10。2014 和 2005 个分级的系统的预兆的能力为 CFS 和 OS 是可比较的(P = 0.321 ) 。然而, 2014 分级的系统没在开始与 PCa 和骨头转移诊断的病人展出优异预兆的功效;trials using 更大的队被要求证实它的预兆的价值。就我们的知识而言,我们的是第一研究在开始诊断的 PCa 把 2005 和 2014 个分级的系统与骨头转移作比较。目前,我们建议两个系统应该被用来与变形 PCa 预言病人的预后。 | Guang-Xi Sun Peng-Fei Shen Xing-Ming Zhang Jing Gong Hao-Jun Gui Kun-Peng Shu Jiang-Dong Liu Jinge Zhao Yao-Jing Yang xue-Qin Chen Ni Chen Hao Zeng | 2017 | Asian Journal of Andrology2017，19，5： | 2 |
| 20 | HIV-1 Vif suppresses antiviral immunity by targeting STING显示文摘HIV-1 infection-induced cGAS–STING–TBK1–IRF3 signaling activates innate immunity to produce type I interferon(IFN).The HIV-1 nonstructural protein viral infectivity factor(Vif)is essential in HIV-1 replication,as it degrades the host restriction factor APOBEC3G.However,whether and how it regulates the host immune response remains to be determined.In this study,we found that Vif inhibited the production of type I IFN to promote immune evasion.HIV-1 infection induced the activation of the host tyrosine kinase FRK,which subsequently phosphorylated the immunoreceptor tyrosine-based inhibitory motif(ITIM)of Vif and enhanced the interaction between Vif and the cellular tyrosine phosphatase SHP-1 to inhibit type I IFN.Mechanistically,the association of Vif with SHP-1 facilitated SHP-1 recruitment to STING and inhibited the K63-linked ubiquitination of STING at Lys337 by dephosphorylating STING at Tyr162.However,the FRK inhibitor D-65495 counteracted the phosphorylation of Vif to block the immune evasion of HIV-1 and antagonize infection.These findings reveal a previously unknown mechanism through which HIV-1 evades antiviral immunity via the ITIM-containing protein to inhibit the posttranslational modification of STING.These results provide a molecular basis for the development of new therapeutic strategies to treat HIV-1 infection. | Yu Wang Gui Qian Lingyan Zhu Zhuo Zhao Yinan Liu Wendong Han Xiaokai Zhang Yihua Zhang Tingrong Xiong Hao Zeng Xianghui Yu Xiaofang Yu Xiaoyan Zhang Jianqing Xu Quanming Zou Dapeng Yan | 2022 | Cellular & Molecular Immunology2022，19，1： | 2 |
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