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| 1 | Collagen type Ⅱ suppresses articular chondrocyte hypertrophy and osteoarthritis progression by promoting integrin β1-SMAD1 interaction显示文摘Hypertrophic differentiation is not only the terminal process of endochondral ossification in the growth plate but is also an important pathological change in osteoarthritic cartilage.Collagen type II(COL2A1)was previously considered to be only a structural component of the cartilage matrix,but recently,it has been revealed to be an extracellular signaling molecule that can significantly suppress chondrocyte hypertrophy.However,the mechanisms by which COL2A1 regulates hypertrophic differentiation remain unclear.In our study,a Col2a1 p.Gly1170Ser mutant mouse model was constructed,and Col2a1 loss was demonstrated in homozygotes.Loss of Col2a1 was found to accelerate chondrocyte hypertrophy through the bone morphogenetic protein(BMP)-SMAD1 pathway.Upon interacting with COL2A1,integrinβ1(ITGB1),the major receptor for COL2A1,competed with BMP receptors for binding to SMAD1 and then inhibited SMAD1 activation and nuclear import.COL2A1 could also activate ITGB1-induced ERK1/2 phosphorylation and,through ERK1/2-SMAD1 interaction,it further repressed SMAD1 activation,thus inhibiting BMP-SMAD1-mediated chondrocyte hypertrophy.Moreover,COL2A1 expression was downregulated,while chondrocyte hypertrophic markers and BMP-SMAD1 signaling activity were upregulated in degenerative human articular cartilage.Our study reveals novel mechanisms for the inhibition of chondrocyte hypertrophy by COL2A1 and suggests that the degradation and decrease in COL2A1 might initiate and promote osteoarthritis progression. | Chengjie Lian Xudong Wang Xianjian Qiu Zizhao Wu Bo Gao Lei Liu Guoyan Liang Hang Zhou Xiaoming Yang Yan Peng Anjing Liang Caixia Xu Dongsheng Huang Peiqiang Su | 2019 | Bone Research2019，7，1： | 17 |
| 2 | A PSO Based Multi-Domain Virtual Network Embedding Approach显示文摘This paper proposed a multi-domain virtual network embedding algorithm based on multi-controller SDN architecture. The local controller first selects candidate substrate nodes for each virtual node in the domain. Then the global controller abstracts substrate network topology based on the candidate nodes and boundary nodes of each domain, and applies Particle Swarm Optimization Algorithm on it to divide virtual network requests. Each local controller then embeds the virtual nodes of the divided single-domain virtual network requests in the domain, and cooperates with other local controllers to embed the inter-domain virtual links. Simulation experimental results show that the proposed algorithm has good performance in reducing embedding cost with good stability and scalability. | Yongjing Ni Guoyan Huang Sheng Wu Chenxi Li Peiying Zhang Haipeng Yao | 2019 | China Communications2019，16，4： | 3 |
| 3 | Sensitive detection of DNA methyltransferase activity based on supercharged fluorescent protein and template-free DNA polymerization显示文摘DNA methylation, catalyzed by DNA methyltransferases(MTases), is a key component of genetic regulation, and DNA MTases have been regarded as potential targets in anticancer therapy. Herein, based on our previously developed DNA-mediated supercharged green fluorescent protein(Sc GFP)/graphene oxide(GO) interaction, coupled with methylation-initiated template-free DNA polymerization, we propose a novel fluorescence assay strategy for sensitive detection of DNA MTase activity. A hairpin DNA with a methylation-sensitive site and an amino-modified 3′-terminal(DNA-1) was designed and worked as a starting molecule. In the presence of DNA MTase, methylation-sensitive restriction endonuclease, and terminal deoxynucleotidyl transferase(Td T), DNA-1 can be sequentially methylated, cleaved, and further elongated. The resulting long DNA fragments quickly bind with Sc GFP and form the Sc GFP/DNA nanocomplex. Such nanocomplex can effectively protect Sc GFP from being adsorbed and quenched by GO. Without the methylation-initiated DNA polymerization, the fluorescence of Sc GFP will be quenched by GO. Thus, the DNA MTase activity, which is proportional to the amount of DNA polymerization products, can be measured by reading the fluorescence of Sc GFP/GO. The method was successfully used to detect the activity of DNA adenine methylation(Dam) MTase with a wide linear range(0.1–100 U/m L) and a low detection limit of 0.1 U/m L. In addition, the method showed high selectivity and the potential to be applied in a complex sample. Furthermore, this study was successfully extended to evaluate the inhibition effect of 5-fluorouracil on Dam MTase activity and detect Td T activity. | Daiqi Li Guoyan Lu Chunyang Lei Zhen Wang Lijun Li Zhou Nie Yan Huang Shouzhuo Yao | 2016 | Science China Chemistry2016，59，7： | 1 |
| 4 | Formation of β-SiC nanowires by annealing SiC films in hydrogen atmosphere显示文摘 | Li Yang Xing Zhang Ru Huang Guoyan Zhang Chengshan Xue | 2006 | Physica E: Low-dimensional Systems and Nanostructures2006，，1： | 1 |
| 5 | Analyses of thermal stress and fracture during cryopreservation of blood vessel显示文摘 | Hua Zezhao Xu Hongyan Zhou Guoyan Liu Jinfen Huang Huimin Ding Wenxiang | 2001 | Science in China Series E: Technolgical Science2001，，2： | 1 |
| 6 | Discussions about Management of Scientific Research Projects in Agricultural Research Institutes: A Case Study of EPPI of CATAS显示文摘Taking Environment and Plant Protection Institute in Chinese Academy of Tropical Agricultural Sciences as an example,it summarized major experience and practice in management of scientific research projects from strengthening project database,enhancing project planning,reinforcing scientific research management team construction,bringing into play functions of academies and institutes,and improving management system. It analyzed existing problems and came up with recommendations from strengthening project process management,improving management team quality,and improving incentive mechanism,in the hope of providing reference for scientific research project management in agricultural scientific research institutes. | Xiaoqiang CHU Hanchun QIN Guoyan SHAN Peiqun LIN Guixiu HUANG | 2015 | Asian Agricultural Research2015，7，5： | 0 |
| 7 | Seasonal snow cover patterns explain alpine treeline elevation better than temperature at regional scale显示文摘Unprecedented modern rates of warming are expected to advance alpine treelines to higher elevations,but global evidence suggests that current treeline dynamics are influenced by a variety of factors.Seasonal snow cover has an essential impact on tree recruitment and growth in alpine regions,which may in turn influence current treeline elevation;however,little research has been conducted on its role in regional treeline formation.Based on 11,804treeline locations in the eastern Himalayas,we extracted elevation,climate,and topographic data for treeline and snowline.Specifically,we used linear and structural equation modelling to assess the relationship between these environmental factors and treeline elevation,and the climate-snow-treeline interaction mechanism.The results showed that the treeline elevation increased with summer temperature and permanent or seasonal snowline elevation,but decreased with snow cover days and spring temperature at the treeline positions(P<0.001).Importantly,spring snowline elevation(33.4%)and seasonal snow cover days(21.1%)contributed the most to treeline elevation,outperforming the permanent snowline,temperature,precipitation,and light.Our results support the assertion that the temperature-moisture interaction affects treeline elevation in the eastern Himalayas,but we also found that the effects were strongly mediated by seasonal snow cover patterns.The increasing tendency of snow cover governed by climate humidification observed in the eastern Himalayas,is likely to limit future treeline advancement and may even cause treeline decline due to the mortality of the remaining old trees.Together,our findings highlight the role of seasonal snow cover patterns in determining treeline elevation in the eastern Himalayas,which should be considered when assessing the potential for treeline ascent in snow-mediated alpine systems elsewhere. | Mengyue Huang Guoyan Wang Xiaojuan Bie Yunqing Jiang Xiyue Huang Jing-Ji Li Songlin Shi Tingbin Zhang Pei-Hao Peng | 2023 | Forest Ecosystems2023，10，2： | 0 |
| 8 | Tone Free Sigma-Delta Modulator with Feedback Dithering for RF Fractional-N Frequency Synthesizer显示文摘 | LIU Junhua LIAO Huailin ZHANG Guoyan HUANG Ru ZHANG Xing | 2007 | Chinese Journal of Electronics2007，16，1： | 0 |
| 9 | A new modeling approach for stress-strain relationship taking into account strain hardening and stored energy by compacted graphite iron evolution显示文摘Compacted graphite iron(CGI)is considered to be an ideal diesel engine material with excellent physical and mechanical properties,which meet the requirements of energy conservation and emission reduction.However,knowledge of the microstructure evolution of CGI and its impact on flow stress remains limited.In this study,a new modeling approach for the stress–strain relationship is proposed by considering the strain hardening effect and stored energy caused by the microstructure evolution of CGI.The effects of strain,strain rate,and deformation temperature on the microstructure of CGI during compression deformation are examined,including the evolution of graphite morphology and the microstructure of the pearlite matrix.The roundness and fractal dimension of graphite particles under different deformation conditions are measured.Combined with finite element simulation models,the influence of graphite particles on the flow stress of CGI is determined.The distributions of grain boundary and geometrically necessary dislocations(GNDs)density in the pearlite matrix of CGI under different strains,strain rates,and deformation temperatures are analyzed by electron backscatter diffraction technology,and the stored energy under each deformation condition is calculated.Results show that the proportion and amount of low-angle grain boundaries and the average GNDs density increase with the increase of strain and strain rate and decreased first and then increased with an increase in deformation temperature.The increase in strain and strain rate and the decrease in deformation temperature contribute to the accumulation of stored energy,which show similar variation trends to those of GNDs density.The parameters in the stress–strain relationship model are solved according to the stored energy under different deformation conditions.The consistency between the predicted results from the proposed stress–strain relationship and the experimental results shows that the evolution of stored energy can accurately predict the stress–strain relationship of CGI. | Jiahui NIU Chuanzhen HUANG Zhenyu SHI Hanlian LIU Zhengyi TANG Binghao LI Zhen CHEN Guoyan JIANG | 2023 | Frontiers of Mechanical Engineering2023，18，4： | 0 |
| 10 | On Agricultural Scientific Research Institutions Supporting and Serving Technological Innovation Entities显示文摘This paper firstly analyzed current situation of agricultural technological innovation system in China and obstacles restricting agricultural enterprises to become technological innovation entities. It discussed exploration and practice of Chinese Academy of Tropical Agricultural Sciences in supporting and serving technological innovation entities. Finally,it came up with ideas and approaches for supporting and serving technological innovation entities in the new trend,to provide a new idea and practice for agricultural scientific research institute supporting and serving technological innovation entities. | Xiaoqiang CHU Bingqiang XU Hanchun QIN Guoyan SHAN Peiqun LIN Guixiu HUANG | 2015 | Asian Agricultural Research2015，7，4： | 0 |
| 11 | Flamelet-like models applied in scramjet combustors:A state of art and prospect显示文摘Developing supersonic combustion models with efficiency,accuracy and practicality is important foundation to deeply understand the complex combustion processes in scramjet engines.Characterized by efficiency and intuition,the flamelet-like models are widely used models in computational combustion methods.However,the supersonic combustion flow field has the nature of strong compressibility,multiple modality,and multiple scales,which poses a great challenge to the traditional flamelet-like models with fixed boundary conditions,and then the complex chemical reaction mechanisms that may face will impose additional computational burden.In this context,this paper reviews the flamelet-like models used in scramjet engines,and summarizes prominent issues in the application practice,including modeling partially premixed combustion,defining progress variable,solving temperature efficiently,evaluating assumed Probability Density Function(PDF)models,and treating mixture fraction variance.Furthermore,possible prospects and directions of improvements are proposed and highlighted for the flamelet-like models.To fully describe the physicochemical scenario and address the raised challenges,these improvements are dedicated to dealing with the compressibility,temperature rise,time-scales,species of interest,multi-inlet combustion,the progress variable definition,and the higher Mach number flight condition. | Tao TANG Zhenguo WANG Yuhui HUANG Mingbo SUN Hongbo WANG Guoyan ZHAO Jiangfei YU | 2023 | Chinese Journal of Aeronautics2023，36，10： | 0 |
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