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| 1 | A Novel QTL qTGW3 Encodes the GSK3/ SHAGGY-Like Kinase OsGSK5/OsSK41 that Interacts with OsARF4 to Negatively Regulate Grain Size and Weight in Rice显示文摘谷物尺寸和形状在米饭是谷物重量和产量的重要决定因素。这里,我们报导一个新主要量的特点在米饭的地点(QTL ) , qTGW3,那种控制谷物尺寸和重量。这个地点, qTGW3,编码 OsSK41 (也作为 OsGSK5 知道) , GLYCOGEN SYNTHASE KINASE 3/SHAGGY-like 家庭的一个成员。带 OsSK41 的 loss-of-function 等位基因的瑞斯 near-isogenic 线增加了谷物长度和重量。我们证明那 OsSK41 交往与并且 phosphorylates 植物生长素反应因素 4 (OsARF4 ) 。有 OsARF4 的 OsSK41 的合作表示在米饭原物增加 OsARF4 的累积。OsARF4 的功能的损失导致更大的米饭谷物。定序 RNA 分析建议 OsARF4 和 OsSK41 镇压下游的基因的一个普通集合的表示, ? 包括一些植物生长素应答的基因,在米饭谷物开发期间。在 qTGW3 的 OsSK41 的 loss-of-function 形式代表没被指向的基因编辑或 QTL 节节上升广泛地在 OsSK41 功能的米饭 breeding.?Suppression 利用了的稀罕等位基因提高米饭谷物尺寸和重量。因此,我们的学习在米饭谷物开发揭示 OsSK41 的重要角色 ?? 并且在另外的谷物庄稼在米饭并且也许为谷物产量的基因改进提供新候选人基因。 | Zejun Hu Sun-Jie Lu Mei-Jing Wang Haohua He Le Sun Hongru Wang Xue-Huan Liu Ling Jiang Jing-Liang sun Xiaoyun Xin Wei Kong Chengcai Chu Hong-Wei Xue Jinshui Yang Xiaojin Luo Jian-Xiang Liu | 2018 | Molecular Plant2018，11，5： | 47 |
| 2 | Target-triggered inhibiting oxidase-mimicking activity of platinum nanoparticles for ultrasensitive colorimetric detection of silver ion显示文摘The development of efficient methods for the detection of hazardous and toxic elements is extremely important for environmental security and public health. In this work, we developed a facile colorimetric assaying system for Ag+ detection in aqueous solution. Chitosan-stabilized platinum nanoparticles(ChPtNPs) were synthesized and severed as an artificial oxidase to catalyze the oxidation of the substrate3,30,5,50-tetramethylbenzidine(TMB) and generate color signal. In the presence of Ag+, due to the strong metallophilic interactions between Ag+ and Pt2+ on the surface of Ch-PtNPs, Ag+ can weaken the affinity to the substrates and inactivate the catalytic activity of Ch-PtNPs, leading to decreased absorbance signal to varying degrees depending on Ag+ amount. Combing the specific binding between Ch-PtNPs and Ag+ with signal amplification procedure based on the Ch-PtNPs-catalyzed TMB oxidation, a sensitive,selective, simple, cost-effective, and rapid detection method for Ag+ can be realized. Ag+ ions in tap and lake waters have been successfully detected. We ensured that the proposed method can be a potential alternative for Ag+ determination in environmental samples. | Haohua Deng Shaobin He Xiuling Lin Lu Yang Zhen Lin Ruiting Chen Huaping Peng Wei Chen | 2019 | Chinese Chemical Letters2019，30，9： | 3 |
| 3 | Adhesive anastomosis for organ transplantation显示文摘The recent development of tough tissue adhesives has stimulated intense interests among material scientists and medical doctors.However,these adhesives have seldom been tested in clinically demanding surgeries.Here we demonstrate adhesive anastomosis in organ transplantation.Anastomosis is commonly conducted by dense sutures and takes a long time,during which all the vessels are occluded.Prolonged occlusion may damage organs and even cause death.We formulate a tough,biocompatible,bioabsorbable adhesive that can sustain tissue tension and pressurized flow.We expose the endothelial surface of vessels onto a gasket,press two endothelial surfaces to the adhesive using a pair of magnetic rings,and reopen the bloodstream immediately.The time for adhesive anastomosis is shortened compared to the time for sutured anastomosis.We have achieved adhesive anastomosis of a great vein in transplanting the liver of a pig.After the surgery,the adhesive is absorbed,the vein heals,and the pig lives for over one month. | Kang Liu Hang Yang Gaobo Huang Aihua Shi Qiang Lu Shanpei Wang Wei Qiao Haohua Wang Mengyun Ke Hongfan Ding Tao Li Yanchao Zhang Jiawei Yu Bingyi Ren Rongfeng Wang Kailing Wang Hui Feng Zhigang Suo Jingda Tang Yi Lv | 2022 | Bioactive Materials2022，7，7： | 3 |
| 4 | miR-495 andmiR-5688 are down-regulated in non-small cell lung cancer under hypoxia to maintain interleukin-11 expression显示文摘Background:Hypoxia is a hallmark of cancer and is associated with poor prognosis.However,the molecular mechanism by which hypoxia promotes tumor progression remains unclear.MicroRNAs dysregulation has been shown to play a critical role in the tumor and tumor microenvironment.Here,we investigated the roles ofmiR-495 and miR-5688 in human non-small cell lung cancer(NSCLC)and their underlying mechanism.Methods:The expression levels of miR-495 and miR-5688 in human NSCLC tissue specimens were measured by quantitative real-time polymerase chain reaction(qRT-PCR).Deferoxamine(DFO)was used to determine whether the regulation of miR-495 and miR-5688 under hypoxia was dependent on hypoxia-inducible factor 1-alpha(HIF-1α).Furthermore,the functions of miR-495 and miR-5688 in tumor progression were evaluated using colony formation,3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2Htetrazolium(MTS),wound healing,transwell assays,and xenograft model.Two algorithms,PicTAR and Targetscan,were used to predict the target gene of these two miRNAs,and dual-luciferase reporter assay was conducted to confirm the target.The unpaired two-tailed t test,Pearson correlation analysis,and Fisher’s exact probability test were performed for statistical analyses.Results:Two miRNAs,miR-495 and miR-5688,were found to participate in NSCLC progression under hypoxia.They were down-regulated in NSCLC tissues compared with normal tissues.We determined that hypoxia led to the down-regulation of miR-495 and miR-5688 in NSCLC cells,which was independent of HIF-1αand cellular metabolic energy.In addition,miR-495 and miR-5688 suppressed cell proliferation,migration,and invasion in vitro.The NSCLC xenograft model showed that miR-495 and miR-5688 inhibited tumor formation in vivo.Interestingly,we found that miR-495 and miR-5688 had the same target,interleukin-11(IL-11).Recombinant human IL-11 counteracted the effects of miR-495 and miR-5688 on NSCLC cells,suggesting that miR-495 and miR-5688 executed their tumor suppressive role by repressing IL-11 expression.Conclusion:We found that hypoxia down-regulated the expression levels of miR-495 and miR-5688 in NSCLCto enhance IL-11 expression and tumor progression,indicating that the miR-495/miR-5688/IL-11 axismay serve as a therapeutic target and potential biomarker for NSCLC. | Meng Zhao Jiao Chang Ran Liu Yahui Liu Jin Qi Yanhui Wang Xinwei Zhang Lu Qiao Yu Jin Haohua An Li Ren | 2020 | Cancer Communications2020，40，9： | 2 |
| 5 | Impact of antitumor regimens on the outcomes of cancer patients with COVID-19:a pooled analysis显示文摘Since the outbreak of coronavirus disease 2019(COVID-19),which is caused by the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)discovered in December 2019,the disease has emerged as a global pandemic(Shi et al.,2020;World Health Organization,2020).Several studies have shown a higher incidence of COVID-19,as well as related poor outcomes in patients with malignancies as compared with those without them(Liang et al.,2020;Tian et al.,2020). | Haohua LU Yu SHI Kelie CHEN Zhi CHEN Haihong ZHU Yuequn NIU Dajing XIA Yihua WU | 2021 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2021，22，10： | 1 |
| 6 | Mechanism of hepatocellular damage in rat caused by low serum selenium显示文摘The aim of this paper is to investigate the mechanism of hepatocellular damage in rats caused by low serum selenium.Thirty six rats were randomly divided into 2 groups:group A(fed with low-selenium diet from the Keshan Disease area with the content of selenium being 0.017 mg/kg);group B[fed with sel-enium-supplemented diet and 0.3 mg/L selenium(Na_(2)SeO_(3))was added to the drinking water].Both were respectively fed for 12 weeks.At the end of the 12th week,the levels of serum selenium,glutathione peroxidase(GPX)and malondialdehyde(MDA)in hepatic tissue were measured;the hepatocellular ultrastructure and apoptosis were observed as well.The levels of serum sel-enium and GPX in group A were markedly lower than those in group B.MDA level in group A was significantly higher than that in group B.Under the electron micro-scope(EM),the mitochondria were remarkably changed in group A.The rate of liver cell apoptosis appeared much higher in group A as well.It indicated that the damage caused by selenium deficiency was through the process of oxidation.Selenium deficiency led to apoptosis of hepa-tocytes where oxidative damage to mitochondria might be the cause. | Yi LU Bo QU Chang LIU Liang YU Xuemin LIU Haohua WANG An JIANG Xiaogang ZHANG | 2008 | Frontiers of Medicine2008，2，3： | 0 |
| 7 | USH2A mutation and specific driver mutation subtypes are associated with clinical efficacy of immune checkpoint inhibitors in lung cancer显示文摘This study aimed to identify subtypes of genomic variants associated with the efficacy of immune checkpoint inhibitors(ICIs)by conducting systematic literature search in electronic databases up to May 31,2021.The main outcomes including overall survival(OS),progression-free survival(PFS),objective response rate(ORR),and durable clinical benefit(DCB)were correlated with tumor genomic features.A total of 1546 lung cancer patients with available genomic variation data were included from 14 studies.The Kirsten rat sarcoma viral oncogene homolog G12C(KRAS^(G12C))mutation combined with tumor protein P53(TP53)mutation revealed the promising efficacy of ICI therapy in these patients.Furthermore,patients with epidermal growth factor receptor(EGFR)classical activating mutations(including EGFRL858Rand EGFRΔ19)exhibited worse outcomes to ICIs in OS(adjusted hazard ratio(HR),1.40;95%confidence interval(CI),1.01-1.95;P=0.0411)and PFS(adjusted HR,1.98;95%CI,1.49-2.63;P<0.0001),while classical activating mutations with EGFR^(T790)Mshowed no difference compared to classical activating mutations without EGFR^(T790)Min OS(adjusted HR,0.96;95%CI,0.48-1.94;P=0.9157)or PFS(adjusted HR,0.72;95%CI,0.39-1.35;P=0.3050).Of note,for patients harboring the Usher syndrome type-2A(USH2A)missense mutation,correspondingly better outcomes were observed in OS(adjusted HR,0.52;95%CI,0.32-0.82;P=0.0077),PFS(adjusted HR,0.51;95%CI,0.38-0.69;P<0.0001),DCB(adjusted odds ratio(OR),4.74;95%CI,2.75-8.17;P<0.0001),and ORR(adjusted OR,3.45;95%CI,1.88-6.33;P<0.0001).Our findings indicated that,USH2A missense mutations and the KRAS^(G12C)mutation combined with TP53 mutation were associated with better efficacy and survival outcomes,but EGFR classical mutations irrespective of combination with EGFR^(T790)Mshowed the opposite role in the ICI therapy among lung cancer patients.Our findings might guide the selection of precise targets for effective immunotherapy in the clinic. | DEXIN YANG YUQIN FENG HAOHUA LU KELIE CHEN JINMING XU PEIWEI LI TIANRU WANG DAJING XIA YIHUA WU | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，2： | 0 |
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