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| 1 | One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces显示文摘编辑技术 CRISPR 的指导 RNA 的 DNA 定期聚类 interspaced 短 palindromic repeats/Cas9 被用来介绍双 stranded 闯入染色体并且在细菌指导随后的地点特定的插入 / 删除或基因材料的代替例如 Escherichia coli,链球菌肺病,和乳杆菌 reuteri。在这研究,我们建立了在 Streptomyces 基因操作为使用编辑 plasmid pKCcas9dO 的一个高效率的 CRISPR/Cas9 染色体,它包括目标特定的指南 RNA,优化 codon 的 cas9,和二个指导相同的修理模板。由交付编辑 plasmids 进模型紧张 Streptomyces coelicolor M145 的 pKCcas9dO 系列,通过一步舞 intergeneric 转移,我们完成了与60%100%的高效率在不同层次编辑的染色体,包括单个基因删除,例如 actII-orf4 ,整顿,和 glnR ,并且单个大尺寸的基因簇删除,扮演 21.3 kb , undecylprodigiosin 红 31.6 kb ,和 Ca 2+-dependent 抗菌素例如 actinorhodin 的抗菌素 biosynthetic 簇 82.8 kb 。而且,我们也分别地与 54% 和 45% 的高效率认识到 actII-orf4 和整顿,并且 ACT 和红 biosynthetic 基因簇的同时的删除。最后,我们使用了这个系统介绍核苷酸点变化进 rpsL 基因,它与抵抗授与异种到链球菌。用这个系统,显著地,为一个轮染色体修正要求的时间被三分之一个减少或为常规方法的那些的一个一半。这些结果清楚地显示实质地编辑系统的确定的 CRISPR/Cas9 染色体改进与当前存在的方法相比编辑效率在的染色体 Streptomyces,和它在另外的放线菌种类为申请有诺言到染色体修正。 | He Huang Guosong Zheng Weihong Jiang Haifeng Hu Yinhua Lu | 2015 | Acta Biochimica et Biophysica Sinica2015，47，4： | 37 |
| 2 | Targeted gene disruption by use of a group 11 intron (targetron) vector in Clostridium acetobutylicum显示文摘 | Lijun Shao Shiyuan Hu Yi Yang Yang Gu Jun Chen Yunliu Yang Weihong Jiang ShengYang | 2007 | Cell Research2007，17，11： | 23 |
| 3 | Signal propagations and linkages of subsurface temperature anomalies in the tropical Pacific and Indian Ocean显示文摘The propagation characteristics of signals along different zonal-time profiles are analyzed using surface and subsurface temperature anomalies over the tropical Pacific and Indian oceans. Analyses show that there are intrinsic relationships between El Nio events in the eastern equatorial Pacific and dipole events in the equatorial Indian Ocean. In the region of tropical North Pacific between the equator and 16°N, there is a circle of propagation of subsurface temperature anomalies. El Nio events only happen when the warm subsurface signals reach the eastern equatorial Pacific. Dipole events are characterized when a warm subsurface signal travels along off-equatorial Indian Ocean to the western boundary. From these analyses, we believe that subsurface temperature anomalies can be considered to be the oceanographic early signal to forecast El Nio events in Pacific Ocean and dipole events in Indian Ocean, respectively. | QIAN Weihong HU Haoran | 2005 | Progress in Natural Science:Materials International2005，15，9： | 8 |
| 4 | Reconstruction of a hybrid nucleoside antibiotic gene cluster based on scarless modification of large DNA fragments显示文摘Genetic modification of large DNA fragments(gene clusters) is of great importance in synthetic biology and combinatorial biosynthesis as it facilitates rational design and modification of natural products to increase their value and productivity.In this study,we developed a method for scarless and precise modification of large gene clusters by using RecET/RED-mediated polymerase chain reaction(PCR) targeting combined with Gibson assembly.In this strategy,the biosynthetic genes for peptidyl moieties(HPHT) in the nikkomycin biosynthetic gene cluster were replaced with those for carbamoylpolyoxamic acid(CPOAA)from the polyoxin biosynthetic gene cluster to generate a^40 kb hybrid gene cluster in Escherichia coli with a reusable targeting cassette.The reconstructed cluster was introduced into Streptomyces lividans TK23 for heterologous expression and the expected hybrid antibiotic,polynik A,was obtained and verified.This study provides an efficient strategy for gene cluster reconstruction and modification that could be applied in synthetic biology and combinatory biosynthesis to synthesize novel bioactive metabolites or to improve antibiotic production. | Jiming Zhuo Binbin Ma Jingjing Xu Weihong Hu Jihui Zhang Huarong Tan Yuqing Tian | 2017 | Science China(Life Sciences)2017，60，9： | 6 |
| 5 | Detection and characterization of diverse alpha-and betacoronaviruses from bats in China显示文摘Bats have been implicated as important reservoir hosts of alpha- and betacoronaviruses. In this study, diverse coronaviruses(CoVs) were detected in 50 of 951(positive rate 5.3%) intestinal specimens of eight bat species collected in four provinces and the Tibet Autonomous Region of China by pan-coronavirus RT-PCR screening. Based on 400-nt RNA-dependent RNA polymerase(Rd RP) sequence analysis, eight belonged to genus Alphacoronavirus and 42 to Betacoronavirus.Among the 50 positive specimens, thirteen gave rise to CoV full-length Rd RP gene amplification for further sequence comparison, of which three divergent sequences(two from a unreported province) were subjected to full genome sequencing. Two complete genomes of betacoronaviruses(JTMC15 and JPDB144) and one nearly-complete genome of alphacoronavirus(JTAC2) were sequenced and their genomic organization predicted. The present study has identified additional numbers of genetically diverse bat-borne coronaviruses with a wide distribution in China. Two new species of bat CoV, identified through sequence comparison and phylogenetic analysis, are proposed. | Lin Xu Fuqiang Zhang Weihong Yang Tinglei Jiang Guanjun Lu Biao He Xingyu Li Tingsong Hu Gang Chen Yun Feng Yuzhen Zhang Quanshui Fan Jiang Feng Hailin Zhang Changchun Tu | 2016 | Virologica Sinica2016，31，1： | 6 |
| 6 | Temporal and spatial cellular and molecular pathological alterations with single-cell resolution in the adult spinal cord after injury显示文摘Spinal cord injury(SCI)involves diverse injury responses in different cell types in a temporally and spatially specific manner.Here,using single-cell transcriptomic analyses combined with classic anatomical,behavioral,electrophysiological analyses,we report,with single-cell resolution,temporal molecular and cellular changes in crush-injured adult mouse spinal cord.Data revealed pathological changes of 12 different major cell types,three of which infiltrated into the spinal cord at distinct times post-injury.We discovered novel microglia and astrocyte subtypes in the uninjured spinal cord,and their dynamic conversions into additional stage-specific subtypes/states.Most dynamic changes occur at 3-days post-injury and by day-14 the second wave of microglial activation emerged,accompanied with changes in various cell types including neurons,indicative of the second round of attacks.By day-38,major cell types are still substantially deviated from uninjured states,demonstrating prolonged alterations.This study provides a comprehensive mapping of cellular/molecular pathological changes along the temporal axis after SCI,which may facilitate the development of novel therapeutic strategies,including those targeting microglia. | Chen Li Zhourui Wu Liqiang Zhou Jingliang Shao Xiao Hu Wei Xu Yilong Ren Xingfei Zhu Weihong Ge Kunshan Zhang Jiping Liu Runzhi Huang Jing Yu Dandan Luo Xuejiao Yang Wenmin Zhu Rongrong Zhu Changhong Zheng Yi Eve Sun Liming Cheng | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 4 |
| 7 | Identifying the northernmost summer monsoon location in East Asia显示文摘能被用来在东亚显示副热带的夏天季风的进展的一个综合索引在这篇论文被建议了。这个索引被三个变量包括降水,风和伪等价物潜力温度联合。极北的夏天季风地点(NSML ) 被每年使用这个索引识别。NSML 经历了,这被发现一内部在时期 1977-1979 的十的移动基于从 1961 ~ 2001 的年度索引分析。有顺序四夏天季风索引的 NSML 的比较也被做了。结果证明 NSML 能很好代表内部在诺思中国的夏天季风降水的年度、内部的十的可变性(在 35 ° N 以外) ,当另外的四个索引能很好沿着长江山谷(约 30 ° N ) | Hu Haoran Qian Weihong | 2007 | Progress in Natural Science:Materials International2007，17，7： | 3 |
| 8 | Long-term exposure to decabrominated diphenyl ether mpairs CD8 T-cell function in adult mice显示文摘 | Weihong Zeng Ying Wang Zhicui Liu Asma Khanniche Qingliang Hu Yan Feng Weiyi Ye Jianglong Yang Shujun Wang Lin Zhou Hao Shen Yan Wang | 2014 | Cellular & Molecular Immunology2014，11，4： | 3 |
| 9 | Extended SRC: Un- dersampled Face Recognition via Intra-Class Variant Dic- tionary显示文摘 | Deng Weihong Hu Jiani Guo Jun | 2012 | IEEE Transactions on Pattern Analysis and Machine Intelligence2012，34，9： | 1 |
| 10 | Evolutionary characteristics of exons expressed at different abundance levels in mammals显示文摘With comparative genomics approaches, we evaluated the evolutionary characteristics of conservation of exons which are expressed abundantly, moderately or lowly in mammals. Using non-coding regions and pseudogenes as controls, sequence identity, phastCons and Ka/Ks analyses were carried out and our results showed that as the exons of high abundance are highly conserved, the minor and low exons also showed conservative characteristics in evolution. Our findings suggested that the exons with less abundance which constitute a large proportion of distinct species in transcriptome of organisms are under functional constraint and might play certain roles in enriching biological complexity in the evolution of organisms. | YU HaiJing FANG Lin LI Xin ZHANG XiaoYan YE Jia LI Jie HU WeiHong ZHENG BingRong XIAO ChunJie | 2009 | Chinese Science Bulletin2009，54，19： | 1 |
| 11 | Extended SRC:Under sam- pled face recognition via infraclass variant dictionary 显示文摘 | Deng Weihong Hu Jiani Guo Jun | 2012 | IEEE transactions on pattern analysis and machine intelli- gence2012，34，9： | 1 |
| 12 | Extended SRC:Undersampled face recognition via intra-class variant dictionary显示文摘 | Deng Weihong Hu Jiani Guo Jun | 2012 | IEEE Transactions on Pattern Analysis and Machine Intelligence2012，34，9： | 1 |
| 13 | The small sample size problem of ICA:A comparative study and analysis显示文摘 | Deng Weihong Liu Yebin Hu Jiani | 2012 | Pattern Recognition2012，45，12： | 1 |
| 14 | Ro- bust, accurate and efficient face recognition from a single training image: A uniform pursuit ap- proach 显示文摘 | Deng Weihong Hu Jiani Guo Jun | 2010 | Pattern Recognition2010，43，5： | 1 |
| 15 | An improved in- cremental singular value decomposition 显示文摘 | Hu Rong Xu Weihong Kuang Fangjun | 2012 | International Journal of Advancements in Computing Technology2012，4，2： | 1 |
| 16 | Efficient transformation of the medicinal mushroomGanoderma lucidum显示文摘 | Li Sun Huaqing Cai Weihong Xu Yuanlei Hu Yin Gao Zhongping Lin | 2001 | Plant Molecular Biology Reporter2001，，： | 1 |
| 17 | Zinc-substituted hemoglobin with specific drug binding sites and fatty acid resistance ability for enhanced photodynamic therapy显示文摘Precisely designed protein-based nanodrugs, as a kind of colloidal drug system, have attracted significant attention in tumor therapy because of their refined drug loading ratio, controlled delivery efficacy and natural biocompatibility. However, most drugs are conjugated to the protein carriers randomly without specific binding sites. Moreover, such sites could easily be replaced by lipophilic molecules in the physiological environment and result in low delivery efficiency. With strong and specific binding locations especially comparatively narrow spatial binding sites and nonflexible structure, hemin (FePPIX)-free hemoglobin or apohemoglobin (apoHb), as a natural metalloporphyrin protein carrier, represents great potential in bioapplication. Therefore, we herein introduce a folate acid (FA) modified, zinc-substituted hemoglobin (ZnPHb-FA) as a naturally occurring protein matrix-based photosensitizer for cancer photodynamic therapy (PDT). Noncovalent inserted ZnPPIX molecules in apoHb possess an extremely stable property and significant recovered photoproperties with superior biocompatibility and phototoxicity, both in vitro and in vivo. This stability was verified by molecular docking analysis and calculation of binding constant, representing a total of five drug binding sites of apoHb for ZnPPIX molecules, four of which are energetically favorable (△G value of -11.9 kcal/mol), and one which is energetically acceptable (△G value of -9 kcal/mol). Folate acid modification has been shown to efficiently enhance the internalization and retention time of ZnPHb nanodrug. ZnPHb-FA is also an efficient depressor of hemin oxygenase-1 (HO-1), which could, in turn, lower the antioxidant ability of cancer cells by decreasing the production of biiirublin. Results in vitro and in vivo both indicated that the firmly combination of apoHb and ZnPPIX described here represents a novel and efficient protein nanodrug systems for cancer therapy. | Yiting Xu Jiamei Xu Xiaoxiao Hu Xin Xia Qian Dong Zhangkun Liu Zhuo Chen Weihong Tan | 2019 | Nano Research2019，12，8： | 1 |
| 18 | Thermocline oscillation and wWarming event in the tropical indian ocean显示文摘 | Qian Weihong Hu Haoran Zhu Yafen | 2003 | Atmosphere-Ocean2003，41，3： | 1 |
| 19 | Extended SRC:undersampled face recognition via intraclass variant dictionary显示文摘 | Deng Weihong Hu Jiani Guo Jun | 2012 | IEEE Trans on Pattern Analysis and Machine Intelligence2012，34，9： | 1 |
| 20 | Centennial-scal dry-wet variations in East Asia显示文摘 | Qian Weihong Hu Qi Zhu Yufan | 2003 | Climate Dynamics2003，21，： | 1 |
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