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| 1 | Targeted gene disruption by use of a group 11 intron (targetron) vector in Clostridium acetobutylicum显示文摘 | Lijun Shao Shiyuan Hu Yi Yang Yang Gu Jun Chen Yunliu Yang Weihong Jiang ShengYang | 2007 | Cell Research2007，17，11： | 23 |
| 2 | Integrated transcriptomic and metabolomic analyses of a wax deficient citrus mutant exhibiting jasmonic acid-mediated defense against fungal pathogens显示文摘Naturally,resistant crop germplasms are important resources for managing the issues of agricultural product safety and environment deterioration.We found a spontaneous mutant of‘Newhall’navel orange(Citrus sinensis Osbeck)(MT)with broad-spectrum protections against fungal pathogens in the orchard,postharvest-storage,and artificial inoculation conditions.To understand the defense mechanism of MT fruit,we constructed a genome-scale metabolic network that integrated metabolome and transcriptome datasets.The coordinated transcriptomic and metabolic data were enriched in two sub-networks,showing the decrease in very long chain fatty acid(by 41.53%)and cuticular wax synthesis(by 81.34%),and increase in the synthesis of jasmonic acid(JA)(by 95.23%)and JA-induced metabolites such as 5-dimethylnobietin(by 28.37%)in MT.Furthermore,cytological and biochemical analyses confirmed that the response to fungal infection in MT was independent of wax deficiency and was correlated with the levels of jasmonates,and the expression of plant defensin gene PDF1.2.Results of exogenous application of MeJA and JA inhibitors such as propyl gallate proved that JA-mediated defense contributes to the strong tolerance against pathogens in MT.Our results indicated that jasmonate biosynthesis and signaling are stimulated by the fatty acid redirection of MT,and participate in the tolerance of pathogenic fungi. | Yizhong He Jingwen Han Runsheng Liu Yuduan Ding Jinqiu Wang Li Sun Xiaoming Yang Yunliu Zeng Weiwei Wen Juan Xu Hongming Zhang Xiang Yan Zhaoxing Chen Zuliang Gu Hong Chen Huanqing Tang Xiuxin Deng Yunjiang Cheng | 2018 | Horticulture Research2018，5，1： | 8 |
| 3 | Gene-lndexed Mutations in Maize显示文摘 | Xiaoduo Lu Jisheng Liu Wen Ren Qun Yang Zhenguang Chai Rumei Chen Lei Wang Jun Zhao Zhihong Lang Haiyang Wang Yunliu Fan Jiuran Zhao Chunyi Zhang | 2018 | Molecular Plant2018，11，3： | 4 |
| 4 | Improving the thermostability of N-carbamyl-d-amino acid amidohydrolase by error-prone PCR显示文摘 | Hong Yu Jian Li Dalong Zhang Yunliu Yang Weihong Jiang Sheng Yang | 2009 | Applied Microbiology and Biotechnology2009，，2： | 1 |
| 5 | Transgenic maize plants expressing a fungal phytase gene显示文摘 | Rumei Chen Guangxing Xue Ping Chen Bin Yao Wenzhu Yang Qianli Ma Yunliu Fan Zuoyu Zhao Mitchell C. Tarczynski Jinrui Shi | 2008 | Transgenic Research2008，，4： | 1 |
| 6 | Transgenic maize plants expressing a fungal phytase gene显示文摘 | Rumei Chen Guangxing Xue Ping Chen Bin Yao Wenzhu Yang Qianli Ma Yunliu Fan Zuoyu Zhao Mitchell C. Tarczynski Jinrui Shi | 2008 | Transgenic Research2008，，4： | 1 |
| 7 | A novel highly acidic β-mannanase from the acidophilic fungus Bispora sp. MEY-1: gene cloning and overexpression in Pichia pastoris显示文摘 | Huiying Luo Yaru Wang Hui Wang Jun Yang Yuhui Yang Huoqing Huang Peilong Yang Yingguo Bai Pengjun Shi Yunliu Fan Bin Yao | 2009 | Applied Microbiology and Biotechnology2009，，3： | 1 |
| 8 | Gene cloning and expression of a new acidic family 7 endo-β-1,3-1,4-glucanase from the acidophilic fungus Bispora sp. MEY-1显示文摘 | Huiying Luo Jun Yang Peilong Yang Jiang Li Huoqing Huang Pengjun Shi Yingguo Bai Yaru Wang Yunliu Fan Bin Yao | 2010 | Applied Microbiology and Biotechnology2010，，4： | 1 |
| 9 | A comprehensive proteomic analysis of elaioplasts from citrus fruits reveals insights into elaioplast biogenesis and function显示文摘Elaioplasts of citrus peel are colorless plastids which accumulate significant amounts of terpenes.However,other functions of elaioplasts have not been fully characterized to date.Here,a LC–MS/MS shotgun technology was applied to identify the proteins from elaioplasts that were highly purified from young fruit peel of kumquat.A total of 655 putative plastid proteins were identified from elaioplasts according to sequence homology in silico and manual curation.Based on functional classification via Mapman,~50%of the identified proteins fall into six categories,including protein metabolism,transport,and lipid metabolism.Of note,elaioplasts contained ATP synthase and ADP,ATP carrier proteins at high abundance,indicating important roles for ATP generation and transport in elaioplast biogenesis.Additionally,a comparison of proteins between citrus chromoplast and elaioplast proteomes suggest a high level of functional conservation.However,some distinctive protein profiles were also observed in both types of plastids notably for isoprene biosynthesis in elaioplasts,and carotenoid metabolism in chromoplasts.In conclusion,this comprehensive proteomic study provides new insights into the major metabolic pathways and unique characteristics of elaioplasts and chromoplasts in citrus fruit. | Man Zhu Jiajia Lin Junli Ye Rui Wang Chao Yang Jinli Gong Yun Liu Chongling Deng Ping Liu Chuanwu Chen Yunjiang Cheng Xiuxin Deng Yunliu Zeng | 2018 | Horticulture Research2018，5，1： | 1 |
| 10 | NEW INSIGHTS INTO THE PHYLOGENY AND SPECIATION OF KUMQUAT (FORTUNELLA SPP.) BASED ON CHLOROPLAST SNP, NUCLEAR SSR AND WHOLE-GENOME SEQUENCING显示文摘Kumquat(Fortunella spp.)is a fruit and ornamental crop worldwide due to the palatable taste and high ornamental value of its fruit.Although Fortunella is classified into the economically important true citrus fruit tree group together with Citrus and Poncirus,few studies have been focused on its evolutionary scenario.In this study,analysis of five chloroplast loci and 47 nuclear microsatellites(nSSR)loci from 38 kumquat and 10 citrus accessions revealed the independent phylogeny of Fortunella among citrus taxa,and that Fortunella mainly comprises two populations:CUL,cultivated Fortunella spp.(F.margarita,F.crassifolia and F.japonica);and HK,wild Hong Kong kumquat(Fortunella hindsii).Genomic analysis based on whole-genome SNPs indicated that the allele frequency of both pupations deviated from the neutral selection model,suggesting directional selection was a force driving their evolutions.CUL exhibited lower genomic diversity and higher linkage strength than HK,suggesting artificial selection involved in its origin.A high level of genetic differentiation(Fst=0.364)was detected and obviously asynchronous demographic changes were observed between CUL and HK.Based on these results,a new hypothesis for the speciation of Fortunella is proposed. | Chenqiao ZHU Peng CHEN Junli YE Hang LI Yue HUANG Xiaoming YANG Chuanwu CHEN Chenglei ZHANG Yuantao XU Xiaoli WANG Xiang YAN Guangzhou DENG Xiaolin JIANG Nan WANG Hongxing WANG Quan SUN Yun LIU Di FENG Min YU Xietian SONG Zongzhou XIE Yunliu ZENG Lijun CHAI Qiang XU Chongling DENG Yunjiang CHENG Xiuxin DENG | 2022 | Frontiers of Agricultural Science and Engineering2022，9，4： | 0 |
| 11 | Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit显示文摘Mitochondria are crucial for the production of primary and secondary metabolites,which largely determine the quality of fruit.However,a method for isolating high-quality mitochondria is currently not available in citrus fruit,preventing high-throughput characterization of mitochondrial functions.Here,based on differential and discontinuous Percoll density gradient centrifugation,we devised a universal protocol for isolating mitochondria from the pulp of four major citrus species,including satsuma mandarin,ponkan mandarin,sweet orange,and pummelo.Western blot analysis and microscopy confirmed the high purity and intactness of the isolated mitochondria.By using this protocol coupled with a label-free proteomic approach,a total of 3353 nonredundant proteins were identified.Comparison of the four mitochondrial proteomes revealed that the proteins commonly detected in all proteomes participate in several typical metabolic pathways(such as tricarboxylic acid cycle,pyruvate metabolism,and oxidative phosphorylation)and pathways closely related to fruit quality(such asγ-aminobutyric acid(GABA)shunt,ascorbate metabolism,and biosynthesis of secondary metabolites).In addition,differentially abundant proteins(DAPs)between different types of species were also identified;these were found to be mainly involved in fatty acid and amino acid metabolism and were further confirmed to be localized to the mitochondria by subcellular localization analysis.In summary,the proposed protocol for the isolation of highly pure mitochondria from different citrus fruits may be used to obtain high-coverage mitochondrial proteomes,which can help to establish the association between mitochondrial metabolism and fruit storability or quality characteristics of different species and lay the foundation for discovering novel functions of mitochondria in plants. | Xin Li Yingfang Chai Hongbin Yang Zhen Tian Chengyang Li Rangwei Xu Chunmei Shi Feng Zhu Yunliu Zeng Xiuxin Deng Pengwei Wang Yunjiang Cheng | 2021 | Horticulture Research2021，8，1： | 0 |
| 12 | Modification of the rib operon derived from Bacillus subtilis and its expression in Escherichia coli显示文摘 | 张会图 Meng Kun Wang Yaru Luo Huiying Yuan Tiezheng Yang Peilong Bai Yingguo Yao Bin Fan Yunliu | 2007 | High Technology Letters2007，13，1： | 0 |
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