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| 1 | Sensitive electrochemical DNA sensor for the detection of HIV based on a polyaniline/graphene nanocomposite显示文摘A nanocomposite of polyaniline/graphene(PAN/GN)was prepared using reverse-phase polymerization.The nanocomposite material was dropcast onto a glassy carbon electrode(GCE).Then,a single-stranded DNA(ssDNA)probe for HIV-1 gene detection was immobilized on the modified electrode,and the negative charged phosphate backbone of the HIV-1 was bound to the modified electrode surface via p-p*stacking interactions.The hybridization between the ssDNA probe and the target HIV-1 formed doublestranded DNA(dsDNA),and the electron transfer resistance of the electrode was measured using impedimetric studies with a[Fe(CN)6]^(3-/4-)redox couple.Under the optimized experimental conditions,the change of the impedance value was linearly related to the logarithm of the concentration of HIV genes in the range from 5.0×10^(-16)M to 1.0×10^(-10)M(R=0.9930),and the HIV sensor exhibited a lower detection limit of 1.0×10^(-16)M(S/N=3).The results show that this biosensor presented wonderful selectivity,sensitivity and specificity for HIV-1 gene detection.Thus,this biosensor provides a new method for the detection of HIV gene fragments. | Qiaojuan Gong Haixia Han Haiying Yang Meiling Zhang Xiaoling Sun Yunxia Liang Zhaorong Liu Wenchan Zhang Jinli Qiao | 2019 | Journal of Materiomics2019，5，2： | 5 |
| 2 | Clinical significance of SOD2 and GSTP1 gene polymorphisms in Chinese patients with gastric cancer显示文摘 | Zhi Xu Haixia Zhu John M. Luk Dongmei Wu Dongying Gu Weida Gong Yongfei Tan Jianwei Zhou Jinhai Tang Zhengdong Zhang Meilin Wang Jinfei Chen | 2012 | Cancer2012，，22： | 2 |
| 3 | Sources, Toxicity and Detection Techniques of Semicarbazide: A Review显示文摘Semicarbazide has become an important pollutant in the environment,but there was no comprehensive literature on its advances.In this review,the source,biotoxicity and detection methods are summarized.Among the sources is the material of chemical reaction,a metabolite of nitrofurazone,food processing(production by azobisformamide and hypochlorite treatment),endogenous substances,etc.Semicarbazide is demonstrated to be accumulative and reproductive toxicity,mutagenicity and genotoxicity,endocrine disruptors,and neurotoxicity.There are still many controversies on the effect of genotoxicity and no clear result on the carcinogenic mechanism.HPLC-MS/MS is the mainstream of the detection methods.Other methods,including HPLC,immunoassay method,biosensor method,electrochemical detection,capillary electrophoresis technique,and spectral techniques mainly,have become less and less in recent years.On all these counts,to reduce the content of semicarbazide in the environment,the key is to control the use of nitrofurazone as the banned drug effectively.Meanwhile the toxicity data should be supplemented to reveal its toxic mechanism. | TIAN Xiuhui LI Huanjun HAN Dianfeng WEN Fenggong LIU Huan LIU Ge PENG Kaixiu GONG Xianghong LIU Xin WANG Weiyun YU Haixia XU Yingjiang | 2021 | Journal of Ocean University of China2021，20，5： | 2 |
| 4 | CUL4B facilitates HBV replication by promoting HBx stabilization显示文摘Objective:Hepatitis B virus(HBV)infection is a major public health problem worldwide.However,the regulatory mechanisms underlying HBV replication remain unclear.Cullin 4 B-RING ubiquitin E3 ligase(CRL4 B)is involved in regulating diverse physiological and pathophysiological processes.In our study,we aimed to explain the role of CUL4 B in HBV infection.Methods:Cul4 b transgenic mice or conditional knockout mice,as well as liver cell lines with CUL4 B overexpression or knockdown,were used to assess the role of CUL4 B in HBV replication.Immunoprecipitation assays and immunofluorescence staining were performed to study the interaction between CUL4 B and HBx.Cycloheximide chase assays and in vivo ubiquitination assays were performed to evaluate the half-life and the ubiquitination status of HBx.Results:The hydrodynamics-based hepatitis B model in Cul4 b transgenic or conditional knockout mice indicated that CUL4 B promoted HBV replication(P<0.05).Moreover,the overexpression or knockdown system in human liver cell lines validated that CUL4 B increased HBV replication in an HBx-dependent manner.Importantly,immunoprecipitation assays and immunofluorescence staining showed an interaction between CUL4 B and HBx.Furthermore,CUL4 B upregulated HBx protein levels by inhibiting HBx ubiquitination and proteasomal degradation(P<0.05).Finally,a positive correlation between CUL4 B expression and HBV pg RNA level was observed in liver tissues from HBV-positive patients and HBV transgenic mice.Conclusions:CUL4 B enhances HBV replication by interacting with HBx and disrupting its ubiquitin-dependent proteasomal degradation.CUL4 B may therefore be a potential target for anti-HBV therapy. | Haixia Shan Bo Wang Xiaodong Zhang Hui Song Xi Li Yongxin Zou Baichun Jiang Huili Hu Hao Dou Changshun Shao Lifen Gao Chunhong Ma Xiaoyun Yang Xiaohong Liang Yaoqin Gong | 2022 | Cancer Biology & Medicine2022，19，1： | 2 |
| 5 | Reverse Apolipoprotein A-I Mimetic Peptide R-D4F Inhibits Neointimal Formation Following Carotid Artery Ligation in Mice显示文摘 | Lin Du Xinkai Qu Haixia Zhen Rujun Li Jun Wang Mingxing Chen Pei Zhao Zhengang Zhang Kaizheng Gong | 2013 | The American Journal of Pathology2013，，： | 1 |
| 6 | A functional polymorphism in MIR 196 A 2 is associated with risk and prognosis of gastric cancer显示文摘 | Shizhi Wang Guoquan Tao Dongmei Wu Haixia Zhu Yan Gao Yongfei Tan Meilin Wang Weida Gong Yan Zhou Jianwei Zhou Zhengdong Zhang | 2013 | Mol Carcinog2013，，1： | 1 |
| 7 | Single-phase interdiffusion in the Ni3Al-Mo ternary system 显示文摘 | Wei Haixia Gong Weiyang Huang Sheng | 2011 | Scripta Materialia2011，64，1： | 1 |
| 8 | Aqueous and hydroalcoholic extracts of Black Maca (Lepidium meyenii) improve scopolamine-induced memory impairment in mice 显示文摘 | Rubio Julio Dang Haixia Gong Mengjuan | 2007 | Food Chem Toxicol2007，45，10： | 1 |
| 9 | Aqueous and hydroalcoholic extracts of Black Maca (Lepidium meyenii) improve scopolamine-induced memory impairment in mice显示文摘 | Julio Rubio Haixia Dang Mengjuan Gong | 2007 | Food and Chemical Toxicology2007，45，10： | 1 |
| 10 | Optimized Design for the Plow of a Submarine Plowing Trencher显示文摘耕作 trencher 的潜水艇的犁是主要功能的机制之一,并且它的优化是很重要的。设计参数在决定一个容器的拖引的力量的要求起一个很重要的作用。一个多客观的基因算法在获得犁的最佳的设计的努力基于犁表面的分析模型被检验了并且适用。为一个特定的土壤条件,草稿力量和是在犁的工作过程的关键参数的犁板表面区域被发现犁片穿入角度的相应最佳的价值和犁的主要的切片的二个表面角度优化。象偏差,犁板电梯角度,正切线的尖变化,和跨越的长度的犁板方面角度那样的参数也沿着土壤流动方向关于犁板表面的力量被分析。结果证明优化的犁有改进的犁表演。分别地,主要的切片和犁板的草稿力量是 10.6% 和 7% 不到原来的设计。犁板的 Gaussian 弯曲的标准差被 64.5% 降低,它暗示优化犁板表面的光滑比原版大得多。 | Hanyang Gong Ruhua Yuan Xiaodong Xing Liquan Wang Zhipeng Wang Haixia Gong | 2013 | Journal of Marine Science and Application2013，12，1： | 1 |
| 11 | Primary malignant tumours and malignant transformation of cysts in the retrorectal space:MRI diagnosis and treatment outcomes显示文摘Background:There are no clear guidelines for the diagnosis and treatment of malignant retrorectal tumours.The purpose of this study was to increase preoperative diagnostic knowledge and to describe the outcomes of treatment for these patients.Methods:This retrospective study was conducted on patients who underwent complete retrorectal tumour resection between May 2006 and July 2018,and had confirmed post-operative pathology reports.Demographic and clinical data(including imaging,perioperative,pathological,and prognostic data)were collected and analysed.Results:Malignant lesions were identified in 15(9[60%],female)patients.The median age of the patients was 59 years(range,34–72 years).Primary malignant tumours were identified in seven patients with solid tumours,in which gastrointestinal stromal tumours accounted for 71.4%(five of seven)and the remainder were chordoma or mucinous adenocarcinoma.Malignant transformation of cysts occurred in another eight patients with heterogeneous tumours,while histopathological features were present in 75%(six of eight)of patients with mucinous adenocarcinoma,and the remainder were squamouscell carcinoma or neuroendocrine tumour(Grade 2).The malignant characteristics of the solid portions observed using magnetic resonance imaging(MRI)were as follows:the cyst wall of the tumour was irregularly thickened;the surface was convex or lobed;the solid tumour had no capsule,or the capsule was destroyed;and the surface had a gyrus-like morphology.At a median follow-up time of 52 months(range,13–100 months),the overall recurrence-free survival rate was 40.0%and the survival rate was 46.7%.Conclusion:Some MRI features can be used to distinguish malignant retrorectal tumours from benign retrorectal tumours.The survival rate of patients with malignant retrorectal tumours is poor. | Jing Gong Yumeng Xu Yan Zhang Lichao Qiao Haixia Xu Ping Zhu Bolin Yang | 2022 | Gastroenterology Report2022，10，1： | 1 |
| 12 | Resistin promotes 3T3-L1 preadipocyte differentiation 显示文摘 | Haixia Gong Yuhui Ni Xirong Guo | 2004 | Eur J Endocrinol2004，250，6： | 1 |
| 13 | Adipose tissue resistin gene expression in DIO and DR rats显示文摘Objective: To investigate the expression of resistin gene in diet-induced obesity (DIO) and diet resistance (DR) rats. Methods: DIO and DR models were prepared with male SD rats after 6 weeks feeding by a diet of relatively high fat, sucrose, and caloric content (HE diet). Body-weight, fat mass, and the concentration of serum insulin were measured, and the expression of resistin and Peroxisome proliferator-activated receptory-γ(PPAR-γ) gene in whit adipose tissue (WAT) was also detected by RT-PCR. Results: ① Body weight, fat mass and the concentration of serum insulin were significantly increased in DIO rats and decreased in DR rats. ② The expression of resistin and PPARγ gene was upregulated in DIO group and supressed in DR group, but the expression of resistin was not detectable in all samples within three groups. Conclusion: Resistin may serve as a link between obesity and insulin resistance, but the individual difference is enormous. | Yuanyuan Zhao Yuhui Ni Xirong Guo Haixia Gong Xia Chi Ronghua Chen | 2006 | Journal of Nanjing Medical University2006，20，1： | 0 |
| 14 | rDNA Sequencing and Preliminary Analysis of Microorganisms from Diseased Edible Fungus Using Non-culture Technology显示文摘The metagenomic DNA of disease tissue samples from four kinds of major edible fungus was extracted by CTAB method combined with DNA gel recovery kit. The genomic DNA was amplified by polymerase chain reaction using the universal primers of 16S rDNA and 18S rDNA,and then monoclonal sequenced after ligated and transformed. rDNA sequences of 20 positive clones were selected randomly from each pair of primers for sequence alignment. The results showed that there were two bacterial diseases and two fungal diseases in the samples,respectively. | Yao Qiang Gong Zhiyuan Gao Neng Han Jiandong Wan Luzhang Ren Pengfei Liu Xiao Huang Chunyan Ren Haixia | 2014 | Plant Diseases and Pests2014，5，1： | 0 |
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