|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Generation of induced secretome from adipose-derived stem cells specialized for disease-specific treatment:An experimental mouse model显示文摘BACKGROUND Recently,the exclusive use of mesenchymal stem cell(MSC)-secreted molecules,named as the secretome,have been evaluated for overcoming the limitations of cell-based therapy while maintaining its advantages.AIM To improve cell-free therapy by adding disease-specificity through stimulation of MSCs using disease-causing materials.METHODS We collected the secretory materials(named as inducers)released from AML12 hepatocytes that had been pretreated with thioacetamide(TAA)and generated the TAA-induced secretome(TAA-isecretome)after stimulating adipose-derived stem cells with the inducers.The TAA-isecretome was intravenously administered to mice with TAA-induced hepatic failure and those with partial hepatectomy.RESULTS TAA-isecretome infusion showed higher therapeutic potential in terms of(1)restoring disorganized hepatic tissue to normal tissue;(2)inhibiting proinflammatory cytokines(interleukin-6 and tumor necrosis factor-α);and(3)reducing abnormally elevated liver enzymes(aspartate aminotransferase and alanine aminotransferase)compared to the naïve secretome infusion in mice with TAA-induced hepatic failure.However,the TAA-isecretome showed inferior therapeutic potential for restoring hepatic function in partially hepatectomized mice.Proteomic analysis of TAA-isecretome identified that antioxidant processes were the most predominant enriched biological networks of the proteins exclusively identified in the TAA-isecretome.In addition,peroxiredoxin-1,a potent antioxidant protein,was found to be one of representative components of TAA-isecretome and played a central role in the protection of TAA-induced hepatic injury.CONCLUSION Appropriate stimulation of adipose-derived stem cells with TAA led to the production of a secretome enriched with proteins,especially peroxiredoxin-1,with higher antioxidant activity.Our results suggest that appropriate stimulation of MSCs with pathogenic agents can lead to the production of a secretome specialized for protecting against the pathogen.This approach is expected to open a new way of developing various specific therapeutics based on the high plasticity and responsiveness of MSCs. | Ok-Hee Kim Ha-Eun Hong Haeyeon Seo Bong Jun Kwak Ho Joong Choi Kee-Hwan Kim Joseph Ahn Sang Chul Lee Say-June Kim | 2020 | World Journal of Stem Cells2020，12，1： | 2 |
| 2 | Enzyme-amplified SERS immunoassay with Ag-Au bimetallic SERS hot spots显示文摘Surface-enhanced Raman scattering(SERS)enables rapid detection of single molecules with high specificity.However,quantitative and sensitive SERS analysis has been a challenge due to the lack of reliable SERS-active materials.In this study,we developed a quantitative SERS-based immunoassay using enzyme-guided Ag growth on Raman labeling compound(RLC)-immobilized gold nanoparticle(Au NP)-assembled silica NPs(SiO2@Au-RLC@Ag).The enzyme amplified Ag+reduction as well as Ag growth on the RLC-immobilized Au NP-assembled silica NPs(SiO2@Au-RLC),which resulted in a significant increase in SERS signal.In the presence of target antigens such as immunoglobulinG(IgG)or prostate-specific antigen(PSA),Ab1-Antigen-Ab2 immune complex with alkaline phosphatase triggered an enzyme-catalyzed reaction to convert 2-phospho-L-ascorbic acid(2-phospho-L-AA)to ascorbic acid(AA).As produced AA reduced Ag+to Ag,forming an Ag hot spot on the surface of SiO2@Au-RLC,which enhanced the SERS signal of SiO2@Au-RLC@Ag in a solution with a target antigen concentration.The plasmonic immunoassay for IgG detection showed a high linearity of SERS intensity in the range of 0.6 to 9.0 ng/mL with a detection limit(LOD)of 0.09 ng/mL,while an LOD of 0.006 ng/mL was obtained for PSA.The results indicate that the sensitivity of our novel SERS-based immunoassay is higher than that of conventional enzyme-based colorimetric immunoassays. | Xuan-Hung Pham Eunil Hahm Tae Han Kim Hyung-Mo Kim Sang Hun Lee Sang Chul Lee Homan Kang Ho-Young Lee Dae Hong Jeong Hak Soo Choi Bong-Hyun Jun | 2020 | Nano Research2020，13，12： | 2 |
| 3 | Clinicopathologic features and prognosis of combined hepatocellular cholangiocarcinoma显示文摘 | Kwang Cheol Koh Hyuk Lee Moon Seok Choi Joon Hyoek Lee Seung Woon Paik Byung Chul Yoo Jong Chul Rhee Jae Won Cho Cheol Keun Park Hong Joo Kim | 2005 | The American Journal of Surgery2005，，1： | 2 |
| 4 | uPAR expression under hypoxic conditions depends on iNOS modulated ERK phosphorylation in the MDA-MB-231 breast carcinoma cell line显示文摘尿激 plasminogen 使活跃之物受体(uPAR ) 戏在癌症侵略和转移和 uPAR 表示的一个主要角色在各种各样的癌症类型与差的预后被相关。而且, uPAR 的表示在 hypoxic 条件下面被增加。氮的氧化物(没有) 并且它可诱导的氮的氧化物 synthase (i NOS ) 生产的代谢物是 hypoxic 应力的重要产品,并且可以不激活或调制细胞外的信号调整 kinase (英皇家空军之阶级最低之兵) 。这里,我们在 MDA-MB-231 人的乳癌房间线在 hypoxic 条件,和 i NOS 并且不的调节效果下面在 A, uPAR,和激活的英皇家空军之阶级最低之兵层次上面评估了。房间在 hypoxic 或 normoxic 孵卵器被孵化并且与 PD98059 对待(一个 MEK 1/2 禁止者,它废除英皇家空军之阶级最低之兵 phosphorylation ) 并且 aminoguanidine (选择 i NOS 禁止者) 。uPAR 表示,英皇家空军之阶级最低之兵 phosphorylation,并且在 A 上面,活动被发现在 hypoxic 条件下面被增加。而且当房间在 hypoxic 条件下面与 PD98059 被对待时, uPAR 低调整,而 aminoguanidine 显著地以一种剂量依赖者方式增加了英皇家空军之阶级最低之兵 phosphorylation。而且, aminoguanidine 增加了 uPAR 表示并且由 PD98059 阻止了 uPAR 表示的抑制。这些结果证明 uPAR 被组织缺氧导致,那增加的 uPAR 表情被英皇家空军之阶级最低之兵 phosphorylation 调停,它被 iNOS/NO 接着在 MDA-MB-231 房间调制。我们结束那 iNOS/NO 在下面经由英皇家空军之阶级最低之兵小径调整在 hypoxic 条件下面调整 uPAR 的表示。 | So Young Yoon Yoo Jung Lee Jae Hong Seo Hwa Jung Sung Kyong Hwa Park In Keun Choi Seok Jin Kim Sang Cheul Oh Chul Won Choi Byung Soo Kim Sang Won Shin Yeul Hong Kim Jun Suk Kim | 2006 | Cell Research2006，16，1： | 2 |
| 5 | How Can We Predict the Presence of Missed Synchronous Lesions After Endoscopic Submucosal Dissection for Early Gastric Cancers or Gastric Adenomas?显示文摘 | Jun Hwan Yoo Sung Jae Shin Kee Myung Lee Jae Myoung Choi Jeong Ook Wi Dong Hoon Kim Sun Gyo Lim Jae Chul Hwang Jae Youn Cheong Byung Moo Yoo Kwang Jae Lee Jin Hong Kim Sung Won Cho | 2013 | Journal of Clinical Gastroenterology2013，，2： | 2 |
| 6 | Ameliorating liver fibrosis in an animal model using the secretome released from miR-122-transfected adipose-derived stem cells显示文摘BACKGROUND Recently,the exclusive use of mesenchymal stem cell(MSC)-secreted molecules,called secretome,rather than cells,has been evaluated for overcoming the limitations of cell-based therapy,while maintaining its advantages.However,the use of na?ve secretome may not fully satisfy the specificity of each disease.Therefore,it appears to be more advantageous to use the functionally reinforced secretome through a series of processes involving physico-chemical adjustments or genetic manipulation rather than to the use na?ve secretome.AIM To determine the therapeutic potential of the secretome released from miR-122-transfected adipose-derived stromal cells(ASCs).METHODS We collected secretory materials released from ASCs that had been transfected with antifibrotic miR-122(MCM)and compared their antifibrotic effects with those of the na?ve secretome(CM).MCM and CM were intravenously administered to the mouse model of thioacetamide-induced liver fibrosis,and their therapeutic potentials were compared.RESULTS MCM infusion provided higher therapeutic potential in terms of:(A)Reducing collagen content in the liver;(B)Inhibiting proinflammatory cytokines;and(C)Reducing abnormally elevated liver enzymes than the infusion of the na?ve secretome.The proteomic analysis of MCM also indicated that the contents of antifibrotic proteins were significantly elevated compared to those in the na?ve secretome.CONCLUSION We could,thus,conclude that the secretome released from miR-122-transfected ASCs has higher antifibrotic and anti-inflammatory properties than the na?ve secretome.Because miR-122 transfection into ASCs provides a specific way of potentiating the antifibrotic properties of ASC secretome,it could be considered as an enhanced method for reinforcing secretome effectiveness. | Kee-Hwan Kim Jae Im Lee Ok-Hee Kim Ha-Eun Hong Bong Jun Kwak Ho Joong Choi Joseph Ahn Tae Yun Lee Sang Chul Lee Say-June Kim | 2019 | World Journal of Stem Cells2019，11，11： | 2 |
| 7 | Usefulness of continuous renal replacement therapy for correcting hypernatremia in a patient with severe congestive heart failure显示文摘 | Hoon Suk Park Yu Ah Hong Hyun Gyung Kim Sun Ryoung Choi In O. Sun Byung Ha Chung Cheol Whee Park Chul Woo Yang Yong Soo Kim Bum Soon Choi | 2012 | Hemodial Int2012，，4： | 1 |
| 8 | Risk factors for perforations associated with endoscopic submucosal dissection in gastric lesions: emphasis on perforation type显示文摘 | Jun Hwan Yoo Sung Jae Shin Kee Myung Lee Jae Myoung Choi Jeong Ook Wi Dong Hoon Kim Sun Gyo Lim Jae Chul Hwang Jae Youn Cheong Byung Moo Yoo Kwang Jae Lee Jin Hong Kim Sung Won Cho | 2012 | Surgical Endoscopy2012，，9： | 1 |
| 9 | Effect of Delayed Transit Time on Arterial Spin Labeling: Correlation With Dynamic Susceptibility Contrast Perfusion Magnetic Resonance in Moyamoya Disease显示文摘 | Tae Jin Yun Chul-Ho Sohn Moon Hee Han Hyun-Seung Kang Jeong Eun Kim Byung-Woo Yoon Jin Chul Paeng Seung Hong Choi Ji-hoon Kim In Chan Song Kee-Hyun Chang | 2013 | Investigative Radiology2013，，11： | 1 |
| 10 | Prediction of cognitive dysfunction and patients’ outcome following valvular heart surgery and the role of cerebral oximetry显示文摘 | Seong Wook Hong Jae Kwang Shim Yong Seon Choi Dae Hee Kim Byung Chul Chang Young Lan Kwak | 2008 | European Journal of Cardio-Thoracic Surgery2008，，4： | 1 |
| 11 | Antiproliferative Effects of Zinc-Citrate Compound on Hormone Refractory Prostate Cancer显示文摘Objective: To investigate the antiproliferative effects of zinc‐citrate compound on hormone refractory prostate cancer (HRPC). Methods: HRPC cell line (DU145) and normal prostate cell line (RWPE-1) were treated with zinc, citrate and zinc-citrate compound at different time intervals and concentrations to investigate the effect of zinc-citrate compound. Mitochondrial (m)-aconitase activity was determined using aconitase assay. DNA laddering analysis was performed to investigate apoptosis of DU145 cells. Molecular mechanism of apoptosis was investigated by Western blot analysis of P53, P21 waf1 , Bcl-2, Bcl-xL and Bax, and also caspase-3 activity analysis. Results: Treatment with zinc-citrate compound resulted in a time-and dose-dependent decrease in cell number of DU145 cells in comparison with RWPE-1. M-aconitase activity was significantly decreased. DNA laddering analysis indicated apoptosis of DU145 cells. Zinc-citrate compound increased the expression of P21 waf1 and P53, and reduced the expression of Bcl-2 and Bcl-xL proteins but induced the expression of Bax protein. Zinc-citrate compound induced apoptosis of DU145 cells by activation of the caspase-3 pathway. Conclusion: Zinc-citrate compound can induce apoptotic cell death in DU145, by caspase-3 activation through up-regulation of apoptotic proteins and down-regulation of antiapoptotic proteins. | Hoo Hong Yong Sun Choi Hyuk Jin Cho Ji Youl Lee Joon Chul Kim Tae Kon Hwang Sae Woong | 2012 | Chinese Journal of Cancer Research2012，24，2： | 1 |
| 12 | Nanog regulates molecules involved in stemness and cell cycle‐signaling pathway for maintenance of pluripotency of P19 embryonal carcinoma stem cells显示文摘 | Seung‐Cheol Choi Ji‐Hyun Choi Chi‐Yeon Park Chul‐Min Ahn Soon‐Jun Hong Do‐Sun Lim | 2012 | J Cell Physiol2012，，11： | 1 |
| 13 | Palliative treatment of malignant gastroduodenal obstruction with metallic stent: prospective comparison of covered and uncovered stents显示文摘 | Kee Myung Lee Sang Jo Choi Sung Jae Shin Jae Chul Hwang Sun Gyo Lim Jae Yeon Jung Byung Moo Yoo Sung Won Cho Jin Hong Kim | 2009 | Scandinavian Journal of Gastroenterology2009，，7： | 1 |
| 14 | Surface plasmon excitation in semitransparent inverted polymer photovoltaic devices and their applications as label-free optical sensors显示文摘Herein,we report on surface plasmon(SP)-sensitive semitransparent inverted polymer photovoltaic(PV)devices that are based on multilayered material systems consisting of poly(3-hexylthiophene):fullerene-derivative bulk-heterojunction PV layers and thin gold or silver anodes.We demonstrate that these PV devices allow the simultaneous generation of both electrical power and SPs on their anodes for photoexcitation just above the optical absorption edge of the PV layers,resulting not only in attenuated total reflection,but also in attenuated photocurrent generation(APG)under the SP resonance(SPR)condition.Moreover,we also confirm that the biomolecular interaction of biotin–streptavidin on the PV devices can be precisely detected via apparent SPR angle shifts in the APG spectra,even without the need for complex attenuated total reflection configurations.We highlight our view that APG measurements made using these PV devices show great potential for the development of future generations of compact and highly sensitive SPR-based optical sensors. | Byoungchoo Park Soo Hong Yun Chan Youn Cho Young Chan Kim Jung Chul Shin Hong Goo Jeon Yoon Ho Huh Inchan Hwang Ku Youn Baik Young In Lee Han Sup Uhm Guang Sup Cho Eun Ha Choi | 2014 | Light(Science & Applications)2014，3，1： | 1 |
| 15 | Insulin resistance is independently associated with peripheral and autonomic neuropathy in Korean type 2 diabetic patients显示文摘 | Kee Ook Lee Ji Sun Nam Chul Woo Ahn Ji-Man Hong Seung-Min Kim Il-Nam Sunwoo Joon-Shik Moon Sang-Jun Na Young-Chul Choi | 2012 | Acta Diabetologica2012，，2： | 1 |
| 16 | Dose effect of cigarette smoking on frequency and spectrum of epidermal growth factor receptor gene mutations in Korean patients with non-small cell lung cancer显示文摘 | Young Joo Lee Hyo Sub Shim Young Ae Kang Su Jung Hong Hyun Ki Kim Hoguen Kim Se Kyu Kim Sung Ho Choi Joo-Hang Kim Byoung Chul Cho | 2010 | Journal of Cancer Research and Clinical Oncology2010，，12： | 1 |
| 17 | Primary Intracerebral Malignant Fibrous Histiocytoma: CT, MRI, and PET‐CT Findings显示文摘 | Roh‐Eul Yoo Seung Hong Choi Sung‐Hye Park Hee‐Won Jung Ji‐hoon Kim Chul‐Ho Sohn Kee‐Hyun Chang | 2011 | Journal of Neuroimaging2011，，1： | 1 |
| 18 | Polymorphisms in the Ghrelin Gene Are Associated with Serum High-Density Lipoprotein Cholesterol Level and not with Type 2 Diabetes Mellitus in Koreans显示文摘 | Hyung Jin Choi Young Min Cho Min Kyong Moon Hye Hun Choi Hyoung Doo Shin Hak Chul Jang Seong Yeon Kim Hong Kyu Lee Kyong Soo Park | 2006 | The Journal of Clinical Endocrinology & Metabolism2006，，11： | 1 |
| 19 | Transplantation of Autologous Chondrocytes Seeded on a Fibrin/Hyaluronan Composite Gel Into Tracheal Cartilage Defects in Rabbits: Preliminary Results显示文摘 | Hyun Jun Hong Jin Seok Lee Jae Won Choi Byoung‐Hyun Min Han‐Bin Lee Chul‐Ho Kim | 2012 | Artificial Organs2012，，11： | 1 |
| 20 | Interaction between warfarin and Korean red ginseng in patients with cardiac valve replacement显示文摘 | Yeon Hong Lee Byung Koo Lee Yoon Jung Choi In Kyung Yoon Byung Chul Chang Hye Sun Gwak | 2009 | International Journal of Cardiology2009，，2： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员