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| 1 | DNA-based vaccination induces humoral and cellular immune responses against hepatitis B virus surface antigen in mice without activation of Cmyc显示文摘AIM To develop a safe and effective DNAvaccine for inducing humoral and cellularimmunological responses against hepatitis Bvirus surface antigen(HBsAg).METHODS BALB/c mice were inoculated withNV-HB/s,a recombinant plasmid that had beeninserted S gene of hepatitis B virus genome andcould express HBsAg in eukaryotes.HBsAgexpression was measured by ABC immunohis-tochemical assay,generation of anti-HBs byELISA and cytotoxic T lymphocyte(CTL),byMTT method,existence of vaccine DNA bySouthern blot hybridization and activation ofoncogene C-myc by in situ hybridization,RESULTS With NV-HB/s vaccination byintramuscular injection,anti-HBs was initiallypositive 2 weeks after inoculation while all micetested were HBsAg positive in the muscles.Thetiters and seroconversion rate of anti-HBs weresteadily increasing as time went on and weredose-dependent.All the mice inoculated with100 μg NV-HB/s were anti-HBs positive onemonth after inoculation,the titer was 1:1024 ormore.The humoral immune response was similarinduced by either intramuscular or intradermalinjection.CTL activities were much stronger(45.26%)in NV-HB/s DNA immunized mice as compared with those(only 6%)in plasma-derived HBsAg vaccine immunized mice.Twomonths after inoculation,all muscle sampleswere positive by Southern-blot hybridization forNV.HB/s DNA detection,but decreased to 25%and all were undetectable by in situhybridization after 6 months.No oncogene C-myc activation was found in the muscle ofinoculation site.CONCLUSION NV-HB/s could generatehumoral and cellular immunological responsesagainst HBsAg that had been safely expressed insitu by NV-HB/s vaccination. | Lian San Zhao Shan Qin Tao You Zhou Hong Tang Li Liu Bing Jun Lei | 2000 | World Journal of Gastroenterology2000，6，2： | 24 |
| 2 | A novel linear microprobe array for the fabrication of neural microelectrodes显示文摘A novel linear microprobe array(LMPA)has been developed by a conventional microfabrication method from silicon.The LMPA leverages the properties of conventional microwire with additional features of naturally formed regular spacing.With the help of periodic microprobe arrays and double-side V-grooves fabricated in advance between each pair of the two microprobes’rear ends,the number of microprobe units for assembly in one array can be flexibly chosen by cleavage fracture from the LMPA.The fabrication method was demonstrated and the prototype device was assessed by electrochemical impedance spectroscopy(EIS)and in vivo test.The SNR of the spikes recorded was 6. | ZHAO Shan Shan PEI Wei Hua ZHAO Hui WANG Yi Jun CHEN San Yuan CHEN Yuan Fang ZHANG He GUO Dong Mei GUI Qiang CHEN Hong Da | 2015 | Science China(Technological Sciences)2015，58，2： | 3 |
| 3 | Clinical Predictors for Diagnosing Pandemic (H1N1) 2009 and Seasonal Influenza (H3N2) in Fever Clinics in Beijing,China显示文摘Objective Symptomatic predictors of influenza could assess risks and improve decisions about isolation and outpatient treatment.To develop such predictors,we undertook a prospective analysis of pandemic (H1N1) 2009 and seasonal influenza (H3N2) in patients attending fever clinics.Methods From 1 May 2009 to 1 January 2010,all adult patients admitted to fever clinics for suspected influenza,confirmed by real time RT-PCR,were enrolled.Predictors of influenza virus infection were selected with logistic regression models.Measures of sensitivity,specificity,positive and negative likelihood ratios (LRs) were calculated to identify the best predictors.Results The clinical features and routine blood test results of influenza (H1N1) 2009 and seasonal influenza were similar.The positive and negative LRs of current US CDC influenza-like illness (ILI) criteria were modest in predicting influenza infection.Our modified clinic predictors improved the ability of the positive and negative LRs to recognize pandemic (H1N1) 2009 and seasonal influenza.The revised criteria are:fever ≥38℃ accompanied by at least one of the following—cough,arthralgia or relative lymphopenia.Conclusion Patients with symptoms and signs that meet the new criteria are likely to have influenza and timely antiviral therapy may be appropriate.In addition,physicians should ascertain if influenza is circulating within the community or if there is a contact history of influenza and combine this information with the newly developed criteria to clinically diagnose influenza. | DAI Xiao Qiu LIU Min ZHANG Tuo Hong YANG Xue Song LI Song Lin LI Xiao Guang LI Yu Ling KADEERBAI Hai San WU Huang | 2012 | Biomedical and Environmental Sciences2012，25，1： | 2 |
| 4 | Kinetic Characteristics of Neutralizing Antibody Responses Vary among Patients with COVID-19显示文摘Objective The coronavirus disease 2019(COVID-19) pandemic continues to present a major challenge to public health. Vaccine development requires an understanding of the kinetics of neutralizing antibody(NAb) responses to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Methods In total, 605 serum samples from 125 COVID-19 patients(from January 1 to March 14, 2020)varying in age, sex, severity of symptoms, and presence of underlying diseases were collected, and antibody titers were measured using a micro-neutralization assay with wild-type SARS-CoV-2.Results NAbs were detectable approximately 10 days post-onset(dpo) of symptoms and peaked at approximately 20 dpo. The NAb levels were slightly higher in young males and severe cases, while no significant difference was observed for the other classifications. In follow-up cases, the NAb titer had increased or stabilized in 18 cases, whereas it had decreased in 26 cases, and in one case NAbs were undetectable at the end of our observation. Although a decreasing trend in NAb titer was observed in many cases, the NAb level was generally still protective.Conclusion We demonstrated that NAb levels vary among all categories of COVID-19 patients. Longterm studies are needed to determine the longevity and protective efficiency of NAbs induced by SARS-CoV-2. | LI Ling Hua TU Hong Wei LIANG Dan WEN Chun Yan LI An An LIN Wei Yin HU Ke Qi HONG Wen Shan LI Yue Ping SU Juan ZHAO San Tao LI Wei YUAN Run Yu ZHOU Ping Ping HU Feng Yu TANG Xiao Ping KE Chang Wen KE Bi Xia CAI Wei Ping | 2021 | Biomedical and Environmental Sciences2021，34，12： | 1 |
| 5 | Multi-category Micro-milling Tool Wear Monitoring with Continu-ous Hidden Markov Models 显示文摘 | ZHU Kunpeng WONG Yoke San HONG Geok Soon | 2009 | Mechanical Systems andSignal Processing2009，23，2： | 1 |
| 6 | Optimized tool path generation for fast tool servo diamond turning of micro-structured surfaces显示文摘 | De Ping Yu Sze Wei Gan Yoke San Wong Geok Soon Hong Mustafizur Rahman Jin Yao | 2012 | The International Journal of Advanced Manufacturing Technology2012，，9： | 1 |
| 7 | A one-model approach to congestion in data envelopment analysis显示文摘 | W.W.Cooper Hong hui Deng Zhimin M.Huang Su san X.Li | | 0，，04： | 1 |
| 8 | Multi--category Micro--milling Tool Wear Moni- toring with Continuous Hidden Markov Models显示文摘 | Zhu Kunpeng Yoke San Wong Geok Soon Hong | 2009 | Mechanical Systems and Signal Processing2009，23，2： | 1 |
| 9 | Wavelet analysis of sensor signals for tool condition monitoring: A review and some new results 显示文摘 | Zhu Kunpeng Wong Yoke San Hong Geok Soon | 2009 | International Journal of Machine Tools & Manufacture2009，49，： | 1 |
| 10 | Increased dietary intake of co-3 polyunsaturated fatty acidsreduces pathological retinal angiogenesis显示文摘 | Kip M C San Giovanni J P Lofqvist C Christopher M A Chen J HiguchiA Hong S Elke A P Majchrzak S Carper D Ann H Kang J X Emily Y C Norman S Charles N S | 2007 | Nature Medicine2007，13，： | 1 |
| 11 | Wavelet analysis of sensor signals for tool condition monitoring: A review and some new results显示文摘 | Kunpeng Zhu Yoke San Wong Geok Soon Hong | 2009 | International Journal of Machine Tools and Manufacture2009，，7： | 1 |
| 12 | Selective sulfoxidation of aryl sulfides by coordinatively unsaturated metalcenters in chromium carboxylate MIL-101 显示文摘 | Hwang Young Kyu Hong Do Young Chang Jong San Seo Hyejin Yoon Minji Kim Jirdaeung Sung Hwa Jlaung Christian Serre G6rard F6rey | 2009 | Applied Catalysis A: General2009，358，： | 1 |
| 13 | High uptakes of CO2 and CH4 in mesoporous metals organic frameworks MIL-100 and MIL-101 显示文摘 | Philip L Llewellyn Sandrine Bourrelly Christian Serre Alexandre Vimont Marco DaRn-i Lomig Hamon Guy De Weireld Chang Jong San Hong Do Young Hwang Young Kyu Sung Hwa Jhung G6rard F6rey | 2008 | Langmuir2008，24，： | 1 |
| 14 | Characterization and expression profiling of cucumber kinesin genes during early fruit development: revealing the roles of kinesins in exponential cell production and enlargement in cucumber fruit显示文摘 | Xue Yong Yang Yan Wang Wei Jie Jiang Xiao Ling Liu Xiao Meng Zhang Hong Jun Yu San Wen Huang Guo Qin Liu | 2013 | Journal of Experimental Botany2013，，14： | 1 |
| 15 | Pharmaco- kinetic, tissue distribution, and excretion of puerarin and puerarin - phospholipid complex in rats显示文摘 | Wei - San Pan Shi - Lin Chen Hong - Xi Xu ct al | 2006 | Drug Dev Ind Pharm2006，32，： | 1 |
| 16 | Multi-category micro-milling tool wear monitoring with continuous hidden Markov models显示文摘 | Kunpeng Zhu Yoke San Wong Geok Soon Hong | 2008 | Mechanical Systems and Signal Processing2008，，2： | 1 |
| 17 | Two Decades of Universal Hepatitis B Vaccination in Taiwan: Impact and Implication for Future Strategies显示文摘 | Yen–Hsuan Ni Li–Min Huang Mei–Hwei Chang Chung–Jen Yen Chun–Yi Lu San–Lin You Jia–Horng Kao Yu–Chen Lin Huey–Ling Chen Hong–Yuan Hsu Ding–Shinn Chen | 2007 | Gastroenterology2007，，4： | 1 |
| 18 | Sensitive and specific detection of phaseolotoxigenic and nontoxigenic strains of Pseudomonas syringae pv. phaseolicola by TaqMan real-time PCR using site-specific recombinase gene sequences显示文摘 | Min Seok Cho Yong Ho Jeon Man Jung Kang Hong Il Ahn Hyung-Jin Baek Young Wang Na Yu Mi Choi Tae San Kim Dong Suk Park | 2009 | Microbiological Research2009，，： | 1 |
| 19 | A novel CHS/ALG bi-layer composite membrane with sustained antimicrobial efficacy used as wound dressing显示文摘A membrane composed of an alginate(ALG) layer and a chitosan(CHS) layer with sustained antimicrobial efficacy was prepared.Ciprofloxacin HC1(CIP) was incorporated into the ALG layer.Morphological feature of the composite membrane was analyzed by scanning electron microscopy(SEM).Water uptake capacity,in vitro drug release,and in vitro antimicrobial activity were evaluated.The composite membrane exhibited perfect binding characteristic between the two layers.The water uptake capacity of all the membranes was above 800%.The CIP could release from the composite membranes for 48 h.The membrane could control the bacterial growth persistently.The results suggested that this CHS/ALG composite membrane incorporated with CIP had the potential for wound dressing application. | Yang Dong Hong Zhuo Liu Lu Xu Gang Li Zi Ning Ma Fei Han Hui Min Yao Yah Hui Sun San Ming Li | 2010 | Chinese Chemical Letters2010，21，8： | 1 |
| 20 | Synthesis and characterization of polyimide/multi - walled carbon nanotube nanocomposites 显示文摘 | MO TE - CHENG WANG HONG - WEN CHEN SAN - YAN | 2008 | Polymer Composites2008，29，4： | 1 |
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