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| 1 | HBV cccDNA in patients' sera as an indicator for HBV reactivation and an early signal of liver damage显示文摘AIM: To evaluate the covalently closed circle DNA (cccDNA)level of hepatitis B virus (HBV) in patients' liver and sera. METHODS: HBV DNA was isolated from patients' liver biopsies and sera. A sensitive real-time PCR method, which is capable of differentiation of HBV viral genomic DNA and cccDNA, was used to quantify the total HBV cccDNA. The total HBV viral DNA was quantitated by real-time PCR using a HBV diagnostic kit (PG Biotech, LTD, Shenzhen, China)described previously. RESULTS: For the first time, we measured the level of HBV DNA and cccDNA isolated from ten HBV patients' liver biopsies and sera. In the liver biopsies, cccDNA was detected from all the biopsy samples. The copy number of cccDNA ranged from from 0.03 to 173.1 per ceil, the copy number of total HBV DNA ranged from 0.08 to 3 717 per cell. The ratio of total HBV DNA to cccDNA ranged from 1 to 3 406. In the sera,cccDNA was only detected from six samples whereas HBV viral DNA was detected from all ten samples. The ratio of cccDNA to total HBV DNA ranged from 0 to 1.77%. To further investigate the reason why cccDNA could only be detected in some patients' sera, we performed longitudinal studies. The cccDNA was detected from the patients' sera with HBV reactivation but not from the patients' sera without HBV reactivation. The level of cccDNA in the sera was correlated with ALT and viral load in the HBV reactivation patients. CONCLUSION: HBV cccDNA is actively transcribed and replicated in some patients' hepatocytes, which is reflected by a high ratio of HBV total DNA vs cccDNA. Detection of cccDNA in the liver biopsy will provide an end-point for the anti-HBV therapy. The occurrence of cccDNA in the sera is an early signal of liver damage, which may be another important clinical parameter. | Johnny Sze | 2004 | World Journal of Gastroenterology2004，10，1： | 107 |
| 2 | PTEN-L is a novel protein phosphatase for ubiquitin dephosphorylation to inhibit PINK1-Parkin-mediated mitophagy显示文摘Mitophagy 是为损坏线粒体的特定的消除的选择 autophagy 的一种重要类型。(PINK1 )导致 PTEN 的通常认为的 kinase 蛋白质 1 催化 ubiquitin (Ub ) 的 phosphorylation 在 PINK1-Parkin-mediated mitophagy 的发作起一个关键作用。(PTEN-L ) 磷酸酶和 tensin 相当或相同的事物(PTEN ) 长是 PTEN 的最新识别的 isoform,与到它的 N 终点的 173 氨基酸的增加。这里,我们报导 PTEN-L 是经由它对 phosphorylated ubiquitin 的蛋白质磷酸酶活动的 mitophagy 的一个新奇否定管理者。我们发现 PTEN-L 在外部 mitochondrial 膜(OMM ) 本地化,而 PTEN-L 的删除支持, PTEN-L 的 overexpression 禁止, mitophagy 由各种各样的损坏线粒体的代理人导致了。机械学地, PTEN-L 能够有效地阻止 Parkin mitochondrial translocation,减少 Parkin phosphorylation,维持它的关上的不活跃的符合构造,并且禁止它的 E3 ligase 活动。更重要地, PTEN-L 在 vivo 减少 phosphorylated ubiquitin (pSer65-Ub ) 的水平,并且在 vitro,磷酸酶试金经由它的蛋白质磷酸酶活动证实那 PTEN-L dephosphorylates pSer65-Ub,独立于它的类脂化合物磷酸酶功能。一起拿,我们的调查结果为 ubiquitin 作为蛋白质磷酸酶表明 PTEN-L 的新奇功能,它抵抗在 mitophagy 正式就职和 mitophagy 的最终的抑制导致前馈控制机制的阻塞的调停 PINK1 的 ubiquitin phosphorylation。因此,理解 PTEN-L 的这新奇功能在控制 mitophagy 的分子的难题提供一关键错过片,在包括象 Parkinsons 那样的 neurodegenerative 混乱的许多重要人的疾病的一个批评过程疾病。 | Liming Wang Yik-Lam Cho Yancheng Tang Jigang Wang Jung-Eun Park Yajun Wu Chunxin Wang Yan Tong Ritu Chawla Jianbin Zhang Yin Shi Shuo Deng Guang Lu Yihua Wu Hayden Weng-Siong Tan Pornteera Pawijit Grace Gui-Yin Lim Hui-Ying Chan Jingzi Zhang Lei Fang Hanry Yu Yih-Cherng Liou Mallilankaraman Karthik Boon-Huat Bay Kah-Leong Lim Siu-Kwan Sze Celestial T. Yap Han-Ming Shen | 2018 | Cell Research2018，28，8： | 25 |
| 3 | Impacts of different drying strategies on drying characteristics,the retention of bio-active ingredient and colour changes of dried Roselle显示文摘The drying kinetics of Roselle(Hibiscus sabdariffa L) of variety Terengganu(UMKL-1) and the quality attribution of Roselle were studied. The experiments were conducted using four different drying methods, including solar greenhouse drying(SD), solar greenhouse with intermittent heat pump drying(SIHP), hot air drying(HA) and heat pump drying(HP). Among the four drying methods, HP achieved the highest drying rate at a range from0.054 g H_2 O·(g DM)^(-1) ·min^(-1) to 0.212 g H_2 O·(g DM)^(-1)·min^(-1) while SD had the lowest drying rate, measured at 0.042 g H_2 O·(g DM)^(-1)·min^(-1).The analysis on colour kinetics revealed that there is no significant colour loss(p > 0.05) observed from HP's dried Roselle. Greater amount of flavonoid compounds i.e. protocatechuic acid was found in SD and SIHP dried finished product whereas HP's dried Roselle contains higher percentage of catechin as compared to other drying methods. | Thing Chai Tham Mei Xiang Ng Shu Hui Gan Lee Suan Chua Ramlan Aziz Luqman Chuah Abdullah Sze Pheng Ong Nyuk Ling Chin Chung Lim Law | 2018 | Chinese Journal of Chemical Engineering2018，26，2： | 7 |
| 4 | 儿童NTRK重排间叶源性肿瘤临床病理学观察显示文摘目的:探讨儿童NTRK重排间叶源性肿瘤临床和病理学特征,以提高对该类疾病的认识。方法:收集上海交通大学医学院附属上海儿童医学中心及新加坡KK Women′s and Children′s Hospital从2017年1月至2019年9月5例手术切除标本,采用EnVision法检测免疫组织化学表型,荧光原位杂交(FISH)检测相关基因并对NTRK基因重排进行克隆性分析。结果:该组5例患儿,3例男性,2例女性。年龄从3个月到13岁,部位包括软组织(膝、胸腔、腹壁)和肾,肿瘤大小为4.5~12.5 cm。组织学检查主要呈梭形细胞肿瘤,浸润性生长,可伴有炎性细胞。免疫表型上,肿瘤细胞阳性表达Pan-TRK,分子检测均存在NTRK基因重排,包括DCTN1-NTRK1的发现。除2例目前接受靶向药物治疗,其余均为无疾病进展病例,随访时间9~29个月。结论:NTRK重排间叶源性肿瘤,其肿瘤发生部位广泛,组织学图像多变。免疫组织化学Pan-TRK可以帮助诊断,NTRK基因检测确认其存在重排是诊断的金标准,对不能完整切除或有复发、转移病例建议靶向药物治疗。 | 殷敏智 马靖 何巧 沈萍 陈洁枫 金晓婷 张忠德 Chik Hong Kuick Chen Huiyi Eileen Hui Qi Ng Sze Jet Aw Kenneth Tou En Chang | 2020 | 中华病理学杂志2020，49，7： | 6 |
| 5 | Capecitabine with radiation is an effective adjuvant therapy in gastric cancers显示文摘AIM:To analyze the outcome of patients who received concurrent capecitabine(Xeloda) and radiation(XRT) compared to the established concurrent 5-fluorouracil(5-FU) with radiation(5FU-RT) and fluoropyrimidine-based chemotherapy alone as adjuvant treatment in gastric cancers.METHODS:All patients with gastric cancers who received adjuvant treatment at the National Cancer Centre Singapore between 1996 and 2006 were reviewed.Treatment outcomes of patients who received XRT were compared with those who had 5FU-RT or chemotherapy alone as adjuvant therapy for gastric cancers.RESULTS:A total of 108 patients were reviewed.Median age at diagnosis was 60.The majority of the patients(64.8%) had advanced stage Ⅲ and Ⅳ disease(with no distant metastasis).All except 4 patients had D2 gastrectomy.Twenty one patients(19.4%) had positive surgical resection margins.Thirty three patients received XRT compared with 52 who had 5FU-RT and 23 who received chemotherapy alone.For the patients in the chemotherapy-only group,all had fluoropyrimidine-based therapy,with added cisplatin in 7 patients and epirubicin in 2 patients.Median recurrence-free survival was longer for the XRT group(52 mo) compared to the 5FU-RT(35 mo) and chemotherapy-only groups(25 mo)(P=0.48).The patients in the XRT group achieved similar median overall survival(53 mo) as the 5FU-RT(54 mo) and the chemotherapy-only groups(44 mo)(P=0.5).CONCLUSION:Capecitabine with concurrent radiation was as effective as concurrent 5FU with radiation or fluoropyrimidine-based chemotherapy alone when used as adjuvant treatment in patients with gastric cancers. | Chee Kian Tham Su Pin Choo Donald Yew Hee Poon Han Chong Toh Simon Yew Kuang Ong Sze Huey Tan Michael Lian Chek Wang Kian Fong Foo | 2010 | World Journal of Gastroenterology2010，16，29： | 6 |
| 6 | The Rice RMR1 Associates with a Distinct Prevacuolar Compartment for the Protein Storage Vacuole Pathway显示文摘从到液泡的 Golgi 仪器或 trans-Golgi 网络(TGN ) 的 vacuolar 蛋白质的运输在植物房间是经由中间的膜界限 prevacuolar 分隔空间(PVC ) 的一个调停受体的过程。排序受体(VSR ) 和受体相同 region-transmembrane domain-RING-H2 (RMR ) 蛋白质的两 vacuolar 被显示了在把存储蛋白质搬运到蛋白质存储液泡(PSV ) 工作,但是很少为 PSV 小径对 PVC 的性质被知道。这里,我们把米饭 RMR1 (OsRMR1 ) 用作一根探针在植物学习 PSV 小径。有特定的 OsRMR1 抗体的 Immunogold 电子显微镜学(他们)证明 OsRMR1 蛋白质在两个大米文化房间和发展中的大米种子与 PVC 的特征在 Golgi 仪器, TGN ,和一个不同细胞器被发现,象蛋白质身体类型 II ( PBII )或在开发大米种子的 PSV 一样。这个细胞器,也在烟草 BY-2 和 Arabidopsis 暂停发现有教养的房间,从 VSR 积极的 multivesicular 是词法上不同的 lysin 的 PVC 或 multivesicular 身体(MVB ) 并且因此为我们说出存储 PVC (sPVC ) 的 PSV 小径代表 PVC。进一步,在 vivo 并且在 vitro,相互作用用蛋白质分泌了进暂停房间表明了的转基因的 BY-2 的文化媒介的截断的 OsRMR1 学习那 OsRMR1 在搬运象 vicilin 一样存储蛋白质作为排序的受体工作。 | Yun Shen Junqi Wang Yu Ding Sze Wan Lo Guillaume Gouzerh Jean-Marc Neuhau Liwen Jiang | 2011 | Molecular Plant2011，4，5： | 5 |
| 7 | K+ Transporter AtCHX17 with Its Hydrophilic C Tail Localizes to Membranes of the Secretory/Endocytic System: Role in Reproduction and Seed Set显示文摘 | Salil Chanroj Senthilkumar Padmanaban Daniel D. Czerny Guang-Yuh Jauh Heven Sze | 2013 | Molecular Plant2013，6，4： | 5 |
| 8 | Randomised controlled trial comparing modified Sano's and narrow band imaging international colorectal endoscopic classifications for colorectal lesions显示文摘AIM To assess the utility of modified Sano′s(MS) vs thenarrow band imaging international colorectal endoscopic(NICE) classification in differentiating colorectal polyps.METHODS Patients undergoing colonoscopy between 2013 and 2015 were enrolled in this trial.Based on the MS or the NICE classifications,patients were randomised for real-time endoscopic diagnosis.This was followed by biopsies,endoscopic or surgical resection.The endoscopic diagnosis was then compared to the final(blinded) histopathology.The primary endpoint was the sensitivity(Sn),specificity(Sp),positive predictive value(PPV) and negative predictive value(NPV) of differentiating neoplastic and non-neoplastic polyps(MSⅡ/Ⅱo/Ⅲa/Ⅲb vs I or NICE 1 vs 2/3).The secondary endpoints were 'endoscopic resectability'(MSⅡ/Ⅱo/Ⅲa vs Ⅰ/Ⅲb or NICE 2 vs 1/3),NPV for diminutive distal adenomas and prediction of post-polypectomy surveillance intervals.RESULTS A total of 348 patients were evaluated.The Sn,Sp,PPV and NPV in differentiating neoplastic polyps from non-neoplastic polyps were,98.9%,85.7%,98.2% and 90.9% for MS;and 99.1%,57.7%,95.4% and 88.2% for NICE,respectively.The area under the receiver operating characteristic curve(AUC) for MS was 0.92(95%CI:0.86-0.98);and AUC for NICE was 0.78(95%CI:0.69,0.88).The Sn,Sp,PPV and NPV in predicting 'endoscopic resectability' were 98.9%,86.1%,97.8% and 92.5% for MS;and 98.6%,66.7%,94.7% and 88.9% for NICE,respectively.The AUC for MS was 0.92(95%CI:0.87-0.98);and the AUC for NICE was 0.83(95%CI:0.75-0.90).The AUC values were statistically different for both comparisons(P = 0.0165 and P = 0.0420,respectively).The accuracy for diagnosis of sessile serrated adenoma/polyp(SSA/P) with high confidence utilizing MS classification was 93.2%.The differentiation of SSA/P from other lesions achieved Sp,Sn,PPV and NPV of 87.2%,91.5%,89.6% and 98.6%,respectively.The NPV for predicting adenomas in diminutive rectosigmoid polyps(n = 150) was 96.6% and 95% with MS and NICE respectively.The calculated accuracy of post-polypectomy surveillance for MS group was 98.2%(167 out of 170) and for NICE group was 92.1%(139 out of 151).CONCLUSION The MS classification outperformed the NICE classification in differentiating neoplastic polyps and predicting endoscopic resectability.Both classifications met ASGE PIVI thresholds. | Leonardo Zorrón Cheng Tao Pu Kuan Loong Cheong Doreen Siew Ching Koay Sze Pheh Yeap Amanda Ovenden Mahima Raju Andrew Ruszkiewicz Philip W Chiu James Y Lau Rajvinder Singh | 2018 | World Journal of Gastrointestinal Endoscopy2018，10，9： | 4 |
| 9 | Feasibility and accuracy of coronary imaging in elderly patients using the 64-row multi-detector computed tomography: a correlation study with conventional coronary angiography显示文摘Background Elderly patients generally have higher occurrence of coronary calcification, increased heart rate and difficulty with prolonged breath-holding. The aim of our study was to investigate the feasibility and accuracy of using 64-row multi-detector computed tomography (MDCT) in the assessment of coronary artery stenoses in elderly patients. Methods One hundred and fifty two patients with suspected or known coronary artery disease were divided into 4 groups according to their age (Group A: 40-49 years,n=34; Group B: 50-59 years, n=57; Group C: 60-69 years, n=48; Group D: 70 years and above; n=13). Coronary CT angiography (CTA) using a 64-row MDCT was performed and the findings were compared with that of conventional coronary angiography (CCA).Using axial images, multi-planar reconstructions (MPR) and maximum intensity projections (MIP), coronary segments of lumen diameter = 1.5mm were analyzed for the presence of significant stenosis (= 50% ). Results Percentages of poor image quality from coronary CTA preventing reliable correlations with CCA were 21%, 14%, 19% and 62% in Groups A to D respectively. Patients in Group D had significantly higher calcium scores compared with the other groups (P<0.001). In patients where CTA images were of acceptable quality, percentages of accurate correlations with CCA were 89.8%, 93.4%, 86.6% and 78.0% for Groups A to D respectively.There were no significant difference in serum creatinine, heart rate and contrast volume between the 4 groups. Conclusions The 64-row MDCT coronary angiography was less accurate and feasible for patients aged 70 years or above due to heavy coronary calcification and inability to perform a satisfactory breath-hold. However, a high diagnostic accuracy with the MDCT is possible in patients aged less than 70 years. | Chee Khoon LIEW Sze Piaw CHIN Tiong Kiam ONG Seyfarth Tobias Yean Yip FONG Choon Kiat ANG Houng Bang LIEW Rapaee ANNUAR Kui Hian SIM | 2006 | Journal of Geriatric Cardiology2006，3，1： | 4 |
| 10 | Oxy-fuel combustion of pulverized coal: Characterization, fundamentals, stabilization and CFD modeling显示文摘 | Lei Chen Sze Zheng Yong Ahmed F. Ghoniem | 2011 | Progress in Energy and Combustion Science2011，，2： | 3 |
| 11 | 提升医学生人文关怀和医患沟通能力的技巧与方法显示文摘为了提升人文关怀和医患沟通的能力,医学生需要加强人文精神熏陶,要学会尊重患者、倾听患者、主动关心患者;学会使用通俗语言解说医学术语,细心观察病情,发现问题及时沟通。对于某些复杂的疾病,可以借助实物或影像资料向患者予以演示和解说。良好的人文关怀与医患沟通,有利于医患合作。本文给出了具体例证,并加以演示和分析。 | 王光耀 王兴华 马勇 吕新华 杨海霞 Teh Siew Hoon Goh Sze Chin Wang Zhi Hang | 2021 | 叙事医学2021，4，3： | 3 |
| 12 | Phase Ⅰ/Ⅱ randomized controlled trial of autologous bone marrow-derived mesenchymal stem cell therapy for chronic stroke显示文摘AIM To examine the safety and efficacy of mesenchymal stem cell(MSC) therapy for intracerebral haemorrhage with neurological dysfunctions for a year.METHODS MSC were ex vivo expanded from 29 mL(17-42 mL) autologous bone marrow. Patients were randomized to have two intravenous injections of autologous MSC or placebos in four weeks apart. Neurological functions and clinical outcomes were monitored before treatment and at 12^(th), 16^(th), 24^(th), 36^(th) and 60^(th) week upon completion of^(th)e treatment. RESULTS A mean of 4.57 × 10~7(range: 1.43 × 10~7-8.40 × 10~7) MSC per infusion was administered accounting to 8.54 × 10~5(2.65 × 10~5-1.45 × 10~6) per kilogram body weight in two occasions. There was neither adverse event at time of administration nor sign of de novo tumour development among patients after monitoring for a year post MSC therapy. Neuro-restoration and clinical improvement in terms of modified Barthel index, functional independence measure and extended Glasgow Outcome Scale were evident among patients having MSC therapy compared to patients receiving placebos. CONCLUSION Intravenous administration of autologous bone marrowderived MSC is safe and has the potential of improving neurological functions in chronic stroke patients with severe disability. | Kam Sze Tsang Chi Ping Stephanie Ng Xian Lun Zhu George Kwok Chu Wong Gang Lu Anil Tejbhan Ahuja Ka Sing Lawrence Wong Ho Keung Ng Wai Sang Poon | 2017 | World Journal of Stem Cells2017，9，8： | 3 |
| 13 | Oxy-fuel combustion of pulverized coal: Characterization, fundamentals, stabilization and CFD modeling显示文摘 | Lei Chen Sze Zheng Yong Ahmed F. Ghoniem | 2011 | Progress in Energy and Combustion Science2011，，2： | 3 |
| 14 | A Toddler with Rhabdomyosarcoma Presenting as Acute Otitis Media with Mastoid Abscess显示文摘Rhabdomyosarcoma (RMS) is a malignant tumor which involves the striated muscle,and it is most common in the pediatric age group.Usually,children with RMS present with persistent ear discharge,aural polyp and hearing loss which are similar to the symptoms seen in chronic otitis media (COM).[1,2] This similarity with COM often delays the diagnosis.The histological diagnosis of RMS is always a challenge because there are many other conditions which exhibit similar characteristic features such as an aural polyp.[3] We discussed an unusual case of RMS in a 15-month-old girl who presented with acute otitis media (AOM) and mastoid abscess.The biopsy of the aural polyp confirmed the diagnosis. | Sze Yin Ng Bee See Goh | 2016 | Chinese Medical Journal2016，，10： | 2 |
| 15 | Tumor-associated Exosomes Are Involved in Hepatocellular Carcinoma Tumorigenesis, Diagnosis, and Treatment显示文摘Hepatocellular carcinoma(HCC)has become a challenging disease worldwide.There are still limitations in the diagnosis and treatment of HCC,and its high metastatic capacity and high recurrence rate are the main reasons for its poor prognosis.The ability of extracellular vesicles(EVs)to transfer functionally-active substances and their widespread presence in almost all body fluids suggest their unprecedented potential in the study of various cancers.The unique physicochemical properties of EVs determine their potential as antitumor vaccines and drug carriers.In the last decade,the study of EVs in HCC has evolved from a single hot topic to a system with considerable scale.This paper summarizes the role of EVs,especially exosomes,in the occurrence,metastasis and tumor immunity of HCC,reviews their applications in tumor diagnosis,prognosis and treatment,describes the pros and cons of these studies,and looks forward towards the future research directions of EVs in HCC. | Hang Wang Liang Yu Peng Huang Yongxu Zhou Wangyang Zheng Nanfeng Meng Risheng He Yi Xu Tey Sze Keong Yunfu Cui | 2022 | Journal of Clinical and Translational Hepatology2022，10，3： | 2 |
| 16 | The battle against nasopharyngeal cancer显示文摘 | Anne W.M. Lee W.T. Ng Y.H. Chan Henry Sze Connie Chan T.H. Lam | 2012 | Radiotherapy and Oncology2012，，3： | 2 |
| 17 | Predictive control of plug-in electric vehicle chargers with photovoltaic integration显示文摘This paper presents a model predictive control(MPC) for off-board plug-in electric vehicle(PEV)chargers with photovoltaic(PV) integration using two-level four-leg inverter topology. The PEV charger is controlled by a unified controller that incorporates direct power and current MPC to dynamically control decoupled active-reactive power flow in a smart grid environment as well as to control PEV battery charging and discharging reliably. PV power generation with maximum power tracking is seamlessly integrated with the power flow control to provide additional power generation. Fast dynamic response and good steady-state performance under all power flow modes and various environmental conditions are evaluated and analyzed. From the results obtained, the charger demonstrates less than 1.5% total harmonic distortion as well as low active and reactive power ripple of less than 7% and 8% respectively on the grid for all power flow modes. The PEV battery also experiences a low charging and discharging current ripple of less than 2.5%. Therefore,the results indicate the successful implementation of the proposed charger and its control for PV integrated offboard PEV chargers. | Adrian Soon Theam TAN Dahaman ISHAK Rosmiwati MOHD-MOKHTAR Sze Sing LEE Nik Rumzi Nik IDRIS | 2018 | Journal of Modern Power Systems and Clean Energy2018，6，6： | 2 |
| 18 | Antifungal and antiviral products of marine organisms显示文摘 | Randy Chi Fai Cheung Jack Ho Wong Wen Liang Pan Yau Sang Chan Cui Ming Yin Xiu Li Dan He Xiang Wang Evandro Fei Fang Sze Kwan Lam Patrick Hung Kui Ngai Li Xin Xia Fang Liu Xiu Yun Ye Guo Qing Zhang Qing Hong Liu Ou Sha Peng Lin Chan Ki Adnan A Bekhit Alaa | 2014 | Applied Microbiology and Biotechnology2014，，8： | 2 |
| 19 | Growth arrest-specific gene 2 suppresses hepatocarcinogenesis by intervention of cell cycle and p53-dependent apoptosis显示文摘BACKGROUND Growth arrest-specific gene 2(GAS2)plays a role in modulating in reversible growth arrest cell cycle,apoptosis,and cell survival.GAS2 protein is universally expressed in most normal tissues,particularly in the liver,but is depleted in some tumor tissues.However,the functional mechanisms of GAS2 in hepatocellular carcinoma(HCC)are not fully defined.AIM To investigate the function and mechanism of GAS2 in HCC.METHODS GAS2 expression in clinic liver and HCC specimens was analyzed by real-time PCR and western blotting.Cell proliferation was analyzed by counting,MTS,and colony formation assays.Cell cycle analysis was performed by flow cytometry.Cell apoptosis was investigated by Annexin V apoptosis assay and western blotting.RESULTS GAS2 protein expression was lower in HCC than in normal tissues.Overexpression of GAS2 inhibited the proliferation of HCC cells with wide-type p53,while knockdown of GAS2 promoted the proliferation of hepatocytes(P<0.05).Furthermore,GAS2 overexpression impeded the G1-to-S cell cycle transition and arrested more G1 cells,particularly the elevation of sub G1(P<0.01).Apoptosis induced by GAS2 was dependent on p53,which was increased by etoposide addition.The expression of p53 and apoptosis markers was further enhanced when GAS2 was upregulated,but became diminished upon downregulation of GAS2.In the clinic specimen,GAS2 was downregulated in more than 60%of HCCs.The average fold changes of GAS2 expression in tumor tissues were significantly lower than those in paired non-tumor tissues(P<0.05).CONCLUSION GAS2 plays a vital role in HCC cell proliferation and apoptosis,possibly by regulating the cell cycle and p53-dependent apoptosis pathway. | Ran-Xu Zhu Alfred Sze Lok Cheng Henry Lik Yuen Chan Dong-Ye Yang Wai-Kay Seto | 2019 | World Journal of Gastroenterology2019，25，32： | 2 |
| 20 | Total Ginsenoside Extract from Panax ginseng Enhances Neural Stem Cell Proliferation and Neuronal Differentiation by Inactivating GSK-3β显示文摘Objective:To study the effects of total ginsenosides(TG)extract from Panax ginseng on neural stem cell(NSC)proliferation and differentiation and their underlying mechanisms.Methods:The migration of NSCs after treatment with various concentrations of TG extract(50,100,or 200μg/mL)were monitored.The proliferation of NSCs was examined by a combination of cell counting kit-8 and neurosphere assays.NSC differentiation mediated by TG extract was evaluated by Western blotting and immunofluorescence staining to monitor the expression of nestin and microtubule associated protein 2(MAP2).The GSK-3β/β-catenin pathway in TG-treated NSCs was examined by Western blot assay.The NSCs with constitutively active GSK-3βmutant were made by adenovirus-mediated gene transfection,then the proliferation and differentiation of NSCs mediated by TG were further verified.Results:TG treatment significantly enhanced NSC migration(P<0.01 or P<0.05)and increased the proliferation of NSCs(P<0.01 or P<0.05).TG mediation also significantly upregulated MAP2 expression but downregulated nestin expression(P<0.01 or P<0.05).TG extract also significantly induced GSK-3βphosphorylation at Ser9,leading to GSK-3βinactivation and,consequently,the activation of the GSK-3β/β-catenin pathway(P<0.01 or P<0.05).In addition,constitutive activation of GSK-3βin NSCs by the transfection of GSK-3βS9 A mutant was found to significantly suppress TG-mediated NSC proliferation and differentiation(P<0.01 or P<0.05).Conclusion:TG promoted NSC proliferation and neuronal differentiation by inactivating GSK-3β. | LIN Kai-li ZHANG Ji CHUNG Hau-lam WU Xin-yi LIU Bin ZHAO Bo-xin SZE Stephen Cho-wing ZHOU Ping-zheng YUNG Ken Kin-lam ZHANG Shi-qing | 2022 | Chinese Journal of Integrative Medicine2022，28，3： | 2 |
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