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| 1 | Multigene editing via CRISPR/Cas9 guided by a single-sgRNA seed in Arabidopsis显示文摘We report that a solo single-guide RNA(sg RNA) seed is capable of guiding Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/CRISPRàassociated 9(CRISRP/Cas_9) to simultaneously edit multiple genes At RPL_(10)A, At RPL_(10)B and At RPL_(10)C in Arabidopsis. Our results also demonstrate that it is possible to use CRISPR/Cas_9 technology to create At RPL_(10) triple mutants which otherwise cannot be generated by conventional genetic crossing. Compared to other conventional multiplex CRISPR/Cas systems, a single sg RNA seed has the advantage of reducing off-target gene-editing. Such a gene editing system might be also applicable to modify other homologous genes, or even less-homologous sequences for multiple gene-editing in plants and other organisms. | Zhiming Yu Qiyuan Chen Weiwei Chen Xian Zhang Fengling Mei Pengcheng Zhang Mei Zhao Xiaohong Wang Nongnong Shi Stephen Jackson Yiguo Hong | 2018 | Journal of Integrative Plant Biology2018，60，5： | 10 |
| 2 | Tunable near-infrared plasmonic perfect absorber based on phase-change materials显示文摘A tunable plasmonic perfect absorber with a tuning range of 650 nm is realized by introducing a 20 nm thick phase-change material Ge2Sb2Te5 layer into the metal–dielectric–metal configuration.The absorption at the plasmonic resonance is kept above 0.96 across the whole tuning range.In this work we study this extraordinary optical response numerically and reveal the geometric conditions which support this phenomenon.This work shows a promising route to achieve tunable plasmonic devices for multi-band optical modulation,communication,and thermal imaging. | Yiguo Chen Xiong Li Xiangang Luo Stefan AMaier Minghui Hong | 2015 | Photonics Research2015，3，3： | 5 |
| 3 | An Optimal Control Strategy for Multi-UAVs Target Tracking and Cooperative Competition显示文摘An optimal control strategy of winner-take-all(WTA)model is proposed for target tracking and cooperative competition of multi-UAVs(unmanned aerial vehicles).In this model,firstly,based on the artificial potential field method,the artificial potential field function is improved and the fuzzy control decision is designed to realize the trajectory tracking of dynamic targets.Secondly,according to the finite-time convergence high-order differentiator,a double closed-loop UAV speed tracking the controller is designed to realize the speed control and tracking of the target tracking trajectory.Numerical simulation results show that the designed speed tracking controller has the advantages of fast tracking,high precision,strong stability and avoiding chattering.Finally,a cooperative competition scheme of multiple UAVs based on WTA is designed to find the minimum control energy from multiple UAVs and realize the optimal control strategy.Theoretical analysis and numerical simulation results show that the model has the fast convergence,high control accuracy,strong stability and good robustness. | Yiguo Yang Liefa Liao Hong Yang Shuai Li | 2021 | IEEE/CAA Journal of Automatica Sinica2021，8，12： | 4 |
| 4 | Comparative WGBS identifies genes that influence non-ripe phenotype in tomato epimutant Colourless non-ripening显示文摘Whole-genome bisulfite sequencing(WGBS)allows single-base resolution and genome-wide profiling of DNA methylation in plants and animals.This technology provides a powerful tool to identify genes that are potentially controlled by dynamic changes of DNA methylation and demethylation.However,naturally occurring epimutants are rare and genes under epigenetic regulation as well as their biological relevances are often difficult to define.In tomato,fruit development and ripening are a complex process that involves epigenetic control.We have taken the advantage of the tomato epimutant Colourless non-ripening(Cnr)and performed comparative mining of the WGBS datasets for the Cnr and Sl CMT3-silenced Cnr fruits.We compared DNA methylation profiles for the promoter sequences of approximately 5,000 bp immediately upstream of the coding region of a list of20 genes.Differentially methylated regions were found for some of these genes.Virus-induced gene silencing(VIGS)of differentially methylated gene Sl DET1 or Sl PDS resulted in unusual brown pigmentation in Cnr fruits.These results suggest that comparative WGBS coupled with VIGS can be used to identify genes that may contribute to the colourless unripe phenotype of fruit in the Cnr epimutant. | Weiwei Chen Zhiming Yu Junhua Kong Hui Wang Yichen Li Mei Zhao Xiaohong Wang Qianqian Zheng Nongnong Shi Pengcheng Zhang Silin Zhong Paul Hunter Mahmut Tor Yiguo Hong | 2018 | Science China(Life Sciences)2018，61，2： | 3 |
| 5 | Phylogenetic Diversity and Ecological Pattern of Ammonia-oxidizing Archaea in the Surface Sediments of the Western Pacific显示文摘 | CAO HUILUO HONG YIGUO LI MENG GU JIDONG | 2011 | Micrnbial Ecology2011，62，： | 1 |
| 6 | The miR157-SPL-CNR module acts upstream of bHLH101 to negatively regulate iron deficiency responses in tomato显示文摘Iron(Fe)homeostasis is critical for plant growth,development,and stress responses.Fe levels are tightly controlled by intricate regulatory networks in which transcription factors(TFs)play a central role.A series of basic helix-loop-helix(b HLH)TFs have been shown to contribute to Fe homeostasis,but the regulatory layers beyond b HLH TFs remain largely unclear.Here,we demonstrate that the SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE(SPL)TF Sl SPL-CNR negatively regulates Fe-deficiency responses in tomato(Solanum lycopersicum)roots.Fe deficiency rapidly repressed the expression of Sl SPL-CNR,and Fe deficiency responses were intensified in two clustered regularly interspaced palindromic repeats(CRISPR)/CRISPRassociated protein 9-generated Sl SPL-CNR knockout lines compared to the wild-type.Comparative transcriptome analysis identified 47 Fe deficiencyresponsive genes the expression of which is negatively regulated by Sl SPL-CNR,one of which,Slb HLH101,helps regulate Fe uptake genes.Sl SPLCNR localizes the nucleus and interacts with the GTAC and BOX 4(ATTAAT)motifs in the Slb HLH101 promoter to repress its expression.Inhibition of Sl SPL-CNR expression in response to Fe deficiency was well correlated with the expression of the micro RNA Slymi R157.Slymi R157-overexpressing tomato lines displayed enhanced Fe deficiency responses,as did Sl SPL-CNR loss-of-function mutants.We propose that the Slymi R157-Sl SPL-CNR module represents a novel pathway that acts upstream of Slb HLH101 to regulate Fe homeostasis in tomato roots. | Huihui Zhu Jiayi Wang Dan Jiang Yiguo Hong Jiming Xu Shaojian Zheng Jianli Yang Weiwei Chen | 2022 | Journal of Integrative Plant Biology2022，64，5： | 1 |
| 7 | Humic sub stances act as electron aeceptor and redox mediator for micro- bial dissimilatory azo reduction by Shewanella decolorationis S12显示文摘 | HONG Yiguo GUO Jon XU Zhicheng | 2007 | Journal of Microbiology and Biotechnology2007，17，3： | 1 |
| 8 | A comparison of primer sets for detecting 16S rRNA and hydrazine oxidoreductase genes of anaerobic ammonium-oxidizing bacteria in marine sediments显示文摘 | Meng Li Yiguo Hong Martin Gunter Klotz Ji-Dong Gu | 2010 | Applied Microbiology and Biotechnology2010，，2： | 1 |
| 9 | Spatial distribution and abundances of ammonia-oxidizing archaea(AOA) and ammonia- oxidizing bacteria (AOB) in mangrove sediments 显示文摘 | Li Meng Cao Huiluo Hong Yiguo | 2011 | Applied Mi- crobiology and Bioteehnology2011，89，4： | 1 |
| 10 | Transcriptional and post‑transcriptional regulation of RNAi‑related gene expression during plant‑virus interactions显示文摘As sessile organisms,plants encounter diverse invasions from pathogens including viruses.To survive and thrive,plants have evolved multilayered defense mechanisms to combat virus infection.RNAi,also known as RNA silencing,is an across-kingdom innate immunity and gene regulatory machinery.Molecular framework and crucial roles of RNAi in antiviral defense have been well-characterized.However,it is largely unknown that how RNAi is transcriptionally regulated to initiate,maintain and enhance cellular silencing under normal or stress conditions.Recently,insights into the transcriptional and post-transcriptional regulation of RNAi-related genes in different physiological processes have been emerging.In this review,we integrate these new findings to provide updated views on how plants modulate RNAi machinery at the(post-)transcriptional level to respond to virus infection. | Qian Gong Yunjing Wang Zhenhui Jin Yiguo Hong Yule Liu | 2022 | Stress Biology2022，2，1： | 1 |
| 11 | Physiology and bio- chemistry of reduction of AZO compounds by Shewanella strains relevant to electron transport chain 显示文摘 | Hong Yiguo Gu Jidong | 2010 | Applied Microbiology and Biotech- nology2010，88，： | 1 |
| 12 | Respiration and growth of Shewanella decolorationis S12 with an Azo Compound as the sole electron acceptor显示文摘 | HONG Yiguo XU Meiying GUO Jun | 2007 | Applied and Environmental Microbiology2007，73，1： | 1 |
| 13 | Spatial assessment of water quality using chemometrics in the Pearl River Estuary, China显示文摘 | Meilin WU Youshao WANG Junde DONG Fulin SUN Yutu WANG Yiguo HONG | 2017 | Frontiers of Earth Science2017，11，1： | 1 |
| 14 | Diversity and abundance of ammonia-oxidizing archaea and bacteria in polluted mangrove sediment显示文摘 | Huiluo Cao Meng Li Yiguo Hong Ji-Dong Gu | 2011 | Systematic and Applied Microbiology2011，，7： | 1 |
| 15 | MFSD12 affects glycosphingolipid metabolism by modulating lysosome homeostasis显示文摘Dear Editor,Glycosphingolipid(GSL)catabolism is strictly regulated in a sequential manner by lysosomal hydrolases with the help of lipid-binding proteins.Impairments of the catabolic pathway cause the onset of lysosomal storage diseases(LsDs),which are characterized by the aberrant accumulation of GSLs(Hannun and Obeid 2018;Breiden and Sandhoff 2019).The primary LSDs occur through the well-known mechanism of inherited defects in genes encoding the catabolic enzymes(or their related cofactors)which reside in the lumen of lysosomes(Hannun and Obeid 2018;Breiden and Sandhoff 2019).However,in some LSDs,the inherited mutations affect other proteins,such as those residing in the lysosomal membrane;these mutations are associated with defects in traffcking and fusion in the endocytic system(Platt et al.2012).However,the non-lumenal lysosomal membrane proteins involved in GSL metabolism remain to be identified. | Yun Hong Zheng Tian Liangjie Jia Yiguo Wang | 2023 | Protein & Cell2023，14，6： | 0 |
| 16 | There is the second virus that causes tobacco leaf curl disease (not TbLCV-CHI) in the field显示文摘Sequence analysis of virus isolation DNA of tobacco leaf curl disease shows that there is the second geminivirus (not Chinese Tobacco Leaf Curl Virus, TbLCV-CHI) that causes tobacco leaf curl disease in the field in the Guangxi Zhuang Autonomous Region, China. This virus DNA-A contains 2 734 nt. Large intergenic region (LIR) contains 269 nt, the virus sense strand contains 2 open reading frames (ORFs): AV1 (115 aa) and AV2 (coat protein gene, CP, 256 aa), and the complementary sense strand contains 4 ORFs: AC1 (replicase gene, 361 aa), AC2 (transactivator, 134 aa), ACS (134 aa) and AC4 (97 aa). The virus belongs to one kind of subgroup III gemini- viruses from old world, and could be the Chinese tomato yellow leaf curl virus (TYLCV-CHI). | GONG Qianhong LIU Yule HONG Yiguo WANG Huanyu CHEN Gang CAI Jianhe | 2000 | Chinese Science Bulletin2000，45，12： | 0 |
| 17 | CG hypermethylation of the bHLH39 promoter regulates its expression and Fe deficiency responses in tomato roots显示文摘Iron(Fe)is an essential micronutrient for all organisms,including plants,whose limited bioavailability restricts plant growth,yield,and nutritional quality.While the transcriptional regulation of plant responses to Fe deficiency have been extensively studied,the contribution of epigenetic modulations,such as DNA methylation,remains poorly understood.Here,we report that treatment with a DNA methylase inhibitor repressed Fe deficiency-induced responses in tomato(Solanum lycopersicum)roots,suggesting the importance of DNA methylation in regulating Fe deficiency responses.Dynamic changes in the DNA methylome in tomato roots responding to short-term(12 hours)and long-term(72 hours)Fe deficiency identified many differentially methylated regions(DMRs)and DMR-associated genes.Most DMRs occurred at CHH sites under short-term Fe deficiency,whereas they were predominant at CG sites following long-term Fe deficiency.Furthermore,no correlation was detected between the changes in DNA methylation levels and the changes in transcript levels of the affected genes under either short-term or long-term treatments.Notably,one exception was CG hypermethylation at the bHLH39 promoter,which was positively correlated with its transcriptional induction.In agreement,we detected lower CG methylation at the bHLH39 promoter and lower bHLH39 expression in MET1-RNA interference lines compared with wild-type seedlings.Virus-induced gene silencing of bHLH39 and luciferase reporter assays revealed that bHLH39 is positively involved in the modulation of Fe homeostasis.Altogether,we propose that dynamic epigenetic DNA methylation in the CG context at the bHLH39 promoter is involved in its transcriptional regulation,thus contributing to the Fe deficiency response of tomato. | Huihui Zhu Guanghao Han Jiayi Wang Jiming Xu Yiguo Hong Li Huang Shaojian Zheng Jianli Yang Weiwei Chen | 2023 | Horticulture Research2023，10，7： | 0 |
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