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| 1 | New mutation points in 23S rRNA gene associated with Helicobacter pyloriresistance to clarithromycin in northeast China显示文摘AIM: TO investigate the resistance rate of Helicobacter pylori(H pylori) to clarithromycin, metronidazole, amoxicillin and tetracycline to guide clinical practice, and to study the mechanism of Hpylori resistant to clarithromycin. METHODS: Thirty Hpyloristrains were isolated from the mucosa of peptic ulcer, gastric tumor and chronic gastritis patients, then the minimal inhibitory concentration (MIC) to clarithromycin, metronidazole, amoxicillin and tetracycline was evaluated by E-test method. The sequence analysis of PCR fragments was conducted in 23S rRNA gene of H pylori resistant to clarithromycin to get the resistance mechanism of the bacteria. RESULTS: Among 30 Hpyloristrains, 7 cases were resistant to clarithromycin, 12 to metronidazole, 2 to tetracycline and no strain was found to be resistant to amoxicillin. The resistance rates were 23.3%, 40%, 6.7% and 0%, respectively. Three new mutation points were found to be related to the clarithromycin resistance in Hpyloriisolates, which were G2224A, C2245T and T2289C. CONCLUSION: In northeast China, H pylori shows high resistance to metronidazole, while sensitive to amoxicillin. The mechanism of resistance to clarithromycin may be related to the mutation of G2224A, C2245T and T2289C in the 23S rRNA gene. | QingHao YanLi Zhi-JieZhang YongLiu HongGao | 2004 | World Journal of Gastroenterology2004，10，7： | 23 |
| 2 | Chromic-P32 phosphate treatment of implanted pancreatic carcinoma: Mechanism involved显示文摘AIM: To study the effects of chromic-P32 phosphate (32p colloids) interstitial administration in Pc-3 implanted pancreatic carcinoma, and investigate its anticancer mechanism.METHODS: Ninety-eight tumor bearing nude mice werekilled at different time points after the injection of 32Pcolloids to the tumor core with observed radioactivity. The light microscopy, transmission electron microscopy (TEM) and immuno-histochemistry and flow cytometry were used to study the rates of tumor cell necrosis, proliferating cell nuclear antigen index, the micro vessel density (MVD). The changes of the biological response to the lymphatic transported 32p colloids in the inguinal lymph node (ILN) were dynamically observed, and the percentage of tumor cell apoptosis, and Apo2.7, caspase-3, Bcl-2, Baxrelated gene expression were observed too.RESULTS: The half-life of effective medication is 13 dafter injection of 32P colloids to the tumor stroma, in 1-6groups, the tumor cell necrosis rates were 20%, 45%,65%, 70%, 95% and 4%, respectively (F= 4.14-105.36, P<0.01). MVD were 38.5±4.0, 28.0±2.9, 17.0±2.9, 8.8±1.5,5.7±2.3 and 65.0±5.2 (t= 11.9-26.1, P<0.01), respectively.Under TEM fairly differentiated Pc-3 cells were found. Thirty days after medication, tumors were shrunk and dried with scabs detached, and those in control group increased in size prominently with plenty of hypodermic blood vessels. In all animals the ILN were enlarged but in medicated animals they appeared later and smaller than those in control group. The extent of irradiative injury in ILN was positively correlated to the dosage of medication. Typical tumor cell apoptosis could be found under TEM inanimals with intra-tumoral injection of low dosed 32P colloids. The peak of apoptosis occurred in 2.96 MBq group and 24 h after irradiation. In the course of irradiationinduced apoptosis, the value of Bcl-2/Bax was down regulated; Apo2.7 and caspase-3 protein expression were prominently increased dose dependently. CONCLUSION: 32p colloids intra-tumor injection having prominent anticancer effectiveness may reveal the ability of promoting cell differentiation. The low dose 32P colloids may induce human pancreatic carcinoma Pc-3 implanted tumor cell apoptosis; Apo2.7, caspase-3, Bcl-2 and Bax protein participated in regulating the process of irradiation induced cell apoptosis. | LuLiu Guo-ShengFeng HongGao Guan-ShengTong YuWang WenGao YingHuang ChengLi | 2005 | World Journal of Gastroenterology2005，11，14： | 8 |
| 3 | Preparation of magnetic polybutylcyanoacrylate nanospheres encapsulated with aclacinomycin A and its effect on gastric tumor显示文摘AIM: To evaluate the effect of aclacinomycin A-loaded magnetic polybutylcyanoacrylate nanoparticles on gastric tumor growth in vivo and in vitro.METHODS: Magnetic polybutylcyanoacrylate (PBCA)nanospheres encapsulated with aclacinomycin A (MPNS-ACM) were prepared by interracial polymerization. Particle size, shape and drug content were examined. Female BABL/c nude mice were implanted with MKN-45 gastric carcinoma tissues subcutaneously to establish human gastric carcinoma model. The mice were randomly divided into 5 groups of 6 each: ACM group (8 mg/kg bin); group of high dosage of MPNS-ACM (8 mg/kg bin); group of low dosage of MPNS-ACM (1.6 mg/kg bin); group of magnetic PBCA nanosphere (MPNS) and control group(normal saline). Magnets (2.5 T) were implanted into the tumor masses in all of the mice one day before the therapy.Above-mentioned drugs were administered intravenously to the mice of every group on the first day and sixth day.When the mice were sacrificed, tumor weight was measured, and the assay of granulocyte- macrophage colony forming-unit (CFU-GM) was performed on semi-solid culture. White blood cell, alanine aminotransferase and creatine were examined. 3-[4-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) was used to examine the viability of MKN-45 cells afger incubation with different concentrations of ACM, MPNS and MPNS-ACM suspension respectively for 48 h.RESULTS: Content of ACM in MPNS-ACM was 12.0% and the average diameter of the particles was 210 nm. The inhibitory rates of ACM (8 mg/kg bin), high dosage of MPNS-ACM (8 mg/kg bin), low dosage of MPNS-ACM (1.6 mg/kg bin)and MPNS on human gastric carcinoma in nude mice were 22.63%, 52.55%, 30.66% and 10.22%, respectively. There was a significant decrease in the number of CFU-GM of bone marrow in ACM group compared with control group,whereas no obvious change was observed in that of the nanosphere groups. The values of 50% inhibition concentration (IC50) of ACM, MPNS and MPNS-ACM were 0.09, 97.78 and 1.07 μg/mL, respectively.CONCLUSION: The tumor inhibitory rate of MPNS-ACM was much higher than that of ACM under magnetic field and the inhibition on bone marrow was alleviated significantly compared with ACM group. | HongGao Ji-YaoWang Xi-ZhongShen Yong-HuiDeng WeiZhang | 2004 | World Journal of Gastroenterology2004，10，14： | 7 |
| 4 | Prehistoric agriculture development in the Yunnan-Guizhou Plateau, southwest China: Archaeobotanical evidence显示文摘The origin, development and expansion of prehistoric agriculture in East Asia have been widely investigated over the past two decades using archaeobotanical analysis from excavated Neolithic and Bronze Age sites. Research on prehistoric agriculture has predominantly focused in the valleys of the Yellow River and the Yangtze River. Agricultural development during the Neolithic and Bronze Age periods in the Yunnan-Guizhou Plateau of southwest China, an important passageway for human migration into Southeast Asia, still remains unclear. In this paper, based on macrofossil and microfossil analysis and radiocarbon dating at the Shilinggang site, we investigate plant subsistence strategies in the Nujiang River valley during the Bronze Age period. Combined with previous archaeobotanical studies in the Yunnan-Guizhou Plateau, we explore agricultural development processes in this area during the Neolithic and Bronze Age. Our results indicate that rice and foxtail millet were cultivated in Shilinggang around 2500 cal a BP. Three phases of prehistoric agricultural development in the Yunnan-Guizhou Plateau can be identified: rice cultivation from 4800–3900 cal a BP, mixed rice and millet crop(foxtail millet and broomcorn millet) cultivation from 3900–3400 cal a BP, and mixed rice, millet crop and wheat cultivation from 3400–2300 cal a BP. The development of agriculture in the Yunnan-Guizhou Plateau during the Neolithic and Bronze Age periods was primarily promoted by prehistoric agriculture expansion across Eurasia, agricultural expansion which was also affected by the topographic and hydrological characteristics of the area. | LI HaiMing ZUO XinXin KANG LiHong REN LeLe LIU FengWen LIU HongGao ZHANG NaiMeng MIN Rui LIU Xu DONG GuangHui | 2016 | Science China Earth Sciences2016，59，8： | 6 |
| 5 | Emergence of ancient cities in relation to geopolitical circumstances and climate change during late Holocene in northeastern Tibetan Plateau, China显示文摘 | Guanghui DONG Honggao LIU Yishi YANG Ying YANG Aifeng ZHOU Zhongxin WANG XiaoyanREN Fahu CHEN | 2016 | Frontiers of Earth Science2016，10，4： | 4 |
| 6 | Establishment of a cell-based assay to screen regulators for Klotho gene promoter显示文摘AIM: To discover compounds which can regulate Klotho promoter activity. Klotho is an aging suppressor gene. A defect in Klotho gene expression in the mouse results in the phenotype similar to human aging. Recombinant Klotho protein improves age-associated diseases in animal models. It has been proposed that up-regulation of Klotho gene expression may have anti-aging effects, METHODS: Klotho promoter was cloned into a vector containing luciferase gene, and the reporter gene vector was transfected into HEK293 cells to make a stable cell line (HEK293/KL). A model for cellular aging was established by treating HEK293/KL cells with H2O2. These cells were treated with extracts from Traditional Chinese Medicines (TCMs). The luciferase activity was detected to identify compounds that can regulate Klotho promoter. RESULTS:The expression of luciferase in these cells was under control of Klotho promoter and down-regulated after H2O2 treatment The down-regulation of luciferase expression was H2O2 concentration-dependent with an IC50 at approximately 0.006%. This result demonstrated that the Klotho gene promoter was regulated by oxidative stress. Using the cell-based reporter gene assay, we screened natural product extracts for regulation of Klotho gene promoter. Several extracts were identified that could rescue the H2O2 effects and up-regulated Klotho promoter activity. CONCLUSION: A cell -based assay for high-throughput drug screening was established to identify compounds that regulate Klotho promoter activity, and several hits were discovered from natural products. Further characterization of these active extracts could help to investigate Klotho function and aging mechanisms. | Zhi-liangXU HongGAO Ke-qingOU-YANG Shao-xiCAI Ying-heHU | 2004 | Acta Pharmacologica Sinica2004，25，9： | 4 |
| 7 | Effects of supercritical carbon dioxide on volatile formation from Maillard reaction between ribose and cysteine显示文摘 | XU Honggao GAO Yanxiang LIU Xuan | | 0，，02： | 1 |
| 8 | 显示文摘 | Xu Ye Wang Dazhi Tang Honggao | 2001 | Materials Characterization2001，47，： | 1 |
| 9 | Comparative analysis of genetic diversity in sacred lotus (Nelumbo nucifera Gaertn.) using AFLP and SSR markers显示文摘 | Jihong Hu Lei Pan Honggao Liu Shuzhen Wang Zhihua Wu Weidong Ke Yi Ding | 2012 | Molecular Biology Reports2012，，4： | 1 |
| 10 | On-line HPLC-ABTS screening and HPLC-DAD-MS/MS identification of free radical scavengers in Gardenia (Gardeniajasminoides Ellis) fruit extracts显示文摘 | HE Wenhao LIU Xuan XU Honggao | 2010 | Food Chemistry2010，123，2： | 1 |
| 11 | Mineral element levels in wild edible mushrooms from Yunnan, China显示文摘 | LIU Honggao ZHANG Ji LI Tao | 2012 | Biological Trace Element Research2012，147，123： | 1 |
| 12 | Preparation of Crystal Oriented α-SiC Films by Pulsed ArF laser Deposition on Si(111)显示文摘 | Wang Yuxia Wen Jun Guo Zhen Tang Honggao | 2000 | Chinese Journal of Semiconductor2000，21，6： | 1 |
| 13 | Effects of ribose to cysteine ratios on the formation of volatile compounds from the Maillard reaction in supercritical carbon dioxide 显示文摘 | Xu Honggao Liuxuan Zhao Jian | 2008 | Food Research International2008，，41： | 1 |
| 14 | Effects of ribose to cysteine ratios on the formation of volatile compounds from the Maillard reaction in supercritical carbon dioxide显示文摘 | XU Honggao LIU Xuan ZHAO Jian | 2008 | Food Research International2008，41，7： | 1 |
| 15 | Molecular cloning and functional analysis of a recombinant ribosome-inactivating protein (alpha-momorcharin) from Momordica charantia显示文摘 | Shuzhen Wang Yubo Zhang Honggao Liu Ying He Junjie Yan Zhihua Wu Yi Ding | 2012 | Applied Microbiology and Biotechnology2012，，4： | 1 |
| 16 | The Green Emission and Local Structure of the Scintillator PbWO4显示文摘 | Qi Zeming Shi Chaoshu Zhou Dongfang Tang Honggao Liu Tao Hu Taidou | 2001 | Physica B2001，307，： | 1 |
| 17 | Effects of ribose to cysteine ratios on the formation of volatile compounds from the Maillard reaction in supercritical carbon dioxide显示文摘 | XU Honggao LIU Xuan ZHAO Jian | 2008 | Food Research International2008，41，7： | 1 |
| 18 | Subcritical water extraction of phenolic compounds from pomegranate ( Punica granatum L.) seed residues and investigation into their antioxidant activities with HPLC–ABTS<ce:glyph name='rad'/> + assay显示文摘 | Li He Xiaofei Zhang Honggao Xu Chao Xu Fang Yuan ?eliko Knez Zoran Novak Yanxiang Gao | 2011 | Food and Bioproducts Processing2011，，2： | 1 |
| 19 | Parallel Data Cube Storage Structure for Range Sum Queries and Dynamic Updates显示文摘I/O parallelism is considered to be a promising approach to achieving high performance in parallel data warehousing systems where huge amounts of data and complex analytical queries have to be processed. This paper proposes a parallel secondary data cube storage structure (PHC for short) to efficiently support the processing of range sum queries and dynamic updates on data cube using parallel computing systems. Based on PHC, two parallel algorithms for processing range sum queries and updates are proposed also. Both the algorithms have the same time complexity, O(loga n/P). The analytical and experimental results show that PHC and the parallel algorithms have high performance and achieve optimum speedup. | HongGao Jian-ZhongLi | 2005 | Journal of Computer Science & Technology2005，20，3： | 0 |
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