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7篇 您的检索式：作者名="Libo Nie"

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		题名	作者	年代	出处	被引量
	1	Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes显示文摘	Qiaozhen Liu Rui Yang Xiuzhen Huang Hui Zhang Lingjuan He Libo Zhang Xueying Tian Yu Nie Shengshou Hu Yan Yan Li Zhang Zengyong Qiao Qing-Dong Wang Kathy O Lui Bin Zhou	2016	Cell Research2016，26，1：	12
	2	Simultaneous extraction of DNA and RNA from Escherichia coli BL 21 based on silica-coated magnetic nanoparticles显示文摘The extraction of nucleic acid is recognized as one of the most essential steps in molecular biology for initiating other downstream applications such as sequencing, amplification, hybridization, and cloning. Many commercial kits and methods are currently available that allow the extraction of only one type of nucleic acids-DNA or RNA. However, in parallel clinical detection of several diseases, a method for simultaneous extraction of both DNA and RNA from the same source is needed in such cases. In this study, a method for simultaneous extraction of DNA and RNA from bacteria based on magnetic nanoparticles(MNPs) was described. Lysis buffers were prepared to help the nucleic acid released and adsorbed to MNPs. Then, two washing buffers were used to remove the contamination of proteins and carbohydrates. The nucleic acids were finally eluted by Deoxyribonuclease(DNase) and Ribonucleases(RNase) free water. Different factors which might affect the purification of the nucleic acid were investigated, and the quantity and quality parameters of the nucleic acid were also recorded. The DNA and RNA extracted from bacteria were then respectively subjected to polymerase chain reaction(PCR) and reverse transcription PCR(RT-PCR) to further confirm its quality. The results indicated that our method can be successfully used to simultaneously extract DNA and RNA from bacteria.	Jiuhai Wang Zeeshan Ali Nianyue Wang Wenbiao Liang Hongna Liu Fu Li Haowen Yang Lei He Libo Nie Nongyue He Zhiyang Li	2015	Science China Chemistry2015，58，11：	5
	3	Size-tunable synthesis of gold nanorods using pyrogallol as a reducing agent显示文摘A novel and facile seed-mediated method for the preparation of monodispersed gold nanorods(GNRs) is presented by introducing pyrogallol as a reductant. Fast Fourier transformation of high-resolution transmission electron microscopy reveals that the synthesized GNRs are single crystalline. The longitudinal surface plasmon resonance of GNRs can be finely tuned by varying silver ion concentrations or seed amounts. Also, both thick(diameter >30 nm) and thin(diameter <10 nm) GNRs with exceptional monodispersity can be well prepared by this method. These findings indicate that this method has a greater performance in controlling the morphology of GNRs than that of traditional approach with ascorbic acid as a reductant.	Yuanfu Huang Kai Xia Nongyue He Zhuoxuan Lu Liming Zhang Yan Deng Libo Nie	2015	Science China Chemistry2015，58，11：	3
	4	Determination of trace amount of antimony  by adsorption voltammetry on carbon paste electrode显示文摘	Guo Huishi Xiao Pengfeng Nie Libo	2004	Journal of Southeast University2004，，：	1
	5	Functional Analysis of Discoidin Domain Receptor 2 in Synovial Fibroblasts in Rheumatoid Arthritis显示文摘	Jicun Wang Houshan Lü Xinping Liu Yanchun Deng Tiezheng Sun Fuyang Li Shaoping Ji Xiaoyan Nie Libo Yao	2002	Journal of Autoimmunity2002，，3：	1
	6	Synthesis of self-crosslinking water soluble acrylic resin and development of tetracolor superimpose printing inks显示文摘	Tang Jianxin Chen Hong Nie Libo		0，，：	1
	7	Synthesis of self cross linking water soluble acrylic resin and development of te extra color super impose printing inks 显示文摘	Tang Jianxin Chen Hong Nie Libo	2003	Journal of Southeast University : Natural Science Editon2003，33，1：	1

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