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| 1 | Simultaneous extraction of DNA and RNA from Escherichia coli BL 21 based on silica-coated magnetic nanoparticles显示文摘The extraction of nucleic acid is recognized as one of the most essential steps in molecular biology for initiating other downstream applications such as sequencing, amplification, hybridization, and cloning. Many commercial kits and methods are currently available that allow the extraction of only one type of nucleic acids-DNA or RNA. However, in parallel clinical detection of several diseases, a method for simultaneous extraction of both DNA and RNA from the same source is needed in such cases. In this study, a method for simultaneous extraction of DNA and RNA from bacteria based on magnetic nanoparticles(MNPs) was described. Lysis buffers were prepared to help the nucleic acid released and adsorbed to MNPs. Then, two washing buffers were used to remove the contamination of proteins and carbohydrates. The nucleic acids were finally eluted by Deoxyribonuclease(DNase) and Ribonucleases(RNase) free water. Different factors which might affect the purification of the nucleic acid were investigated, and the quantity and quality parameters of the nucleic acid were also recorded. The DNA and RNA extracted from bacteria were then respectively subjected to polymerase chain reaction(PCR) and reverse transcription PCR(RT-PCR) to further confirm its quality. The results indicated that our method can be successfully used to simultaneously extract DNA and RNA from bacteria. | Jiuhai Wang Zeeshan Ali Nianyue Wang Wenbiao Liang Hongna Liu Fu Li Haowen Yang Lei He Libo Nie Nongyue He Zhiyang Li | 2015 | Science China Chemistry2015，58，11： | 5 |
| 2 | Abundance and diversity of ammonia-oxidizing archaea in response to various habitats in Pearl River Delta of China, a subtropical maritime zone显示文摘Ammonia-oxidizing archaea(AOA) are widely considered key to ammonia oxidation in various environments.However,little work has been conducted to simultaneously investigate the abundance and diversity of AOA as well as correlations between archaeal amoA genotypes and environmental parameters of different ecosystems at one district.To understand the abundance,diversity,and distribution of AOA in Pearl River Delta of China in response to various habitats,the archaeal amoA genes in soil,marine,river,lake,hot spring and wastewater treatment plant(WWTP) samples were investigated using real-time fluorescent quantitative PCR and clone libraries.Our analyses indicated that the diversity of AOA in various habitats was different and could be clustered into five major clades,i.e.,estuary sediment,marine water/sediment,soil,hot spring and Cluster 1.Phylogenetic analyses revealed that the structure of AOA communities in similar ecological habitats exhibited strong relation.The canonical correspondence method indicated that the AOA community structure was strongly correlated to temperature,pH,total organic carbon,total nitrogen and dissolved oxygen variables.Assessing AOA amoA gene copy numbers,ranging from 6.84 × 106 to 9.45 × 107 copies/g in dry soil/sediment,and 6.06 × 106 to 2.41 × 107 copies/L in water samples,were higher than ammonia-oxidizing bacteria(AOB) by 1-2 orders of magnitude.However,AOA amoA copy numbers were much lower than AOB in WWTP activated sludge samples.Overall,these studies suggested that AOA may be a major contributor to ammonia oxidation in natural habitats but play a minor role in highly aerated activated sludge.The result also showed the ratio of AOA to AOB amoA gene abundance was positively correlated with temperature and less correlated with other environmental parameters.New data from our study provide increasing evidence for the relative abundance and diversity of ammonia-oxidizing archaea in the global nitrogen cycle. | Zhixin Li Wenbiao Jin Zhaoyun Liang Yangyang Yue Junhong Lv | 2013 | Journal of Environmental Sciences2013，25，6： | 3 |
| 3 | Advances of high-performance LiNi_(1-x-y)Co_(x)M_(y)O_(2) cathode materials and their precursor particles via co-precipitation process显示文摘Layered LiNi_(1-x-y)Co_(x)M_(y)O_(2)(M=Mn or Al)is a promising cathode material for lithium-ion batteries due to its high specific capacity and acceptable manufacturing cost.However,the polycrystalline LiNi_(1-x-y)Co_(x)M_(y)O_(2) cathode material suffers from disordered orientation of primary particles and poor geometric symmetry of secondary particles,which severely hampers the migration of Lit ions.Furthermore,the resulting anisotropy accelerates the disintegration of the secondary particle structure,significantly affecting the electrochemical performance of the polycrystalline cathode.In spite of less grain boundary,the single-crystal LiNi_(1-x-y)Co_(x)M_(y)O_(2) cathodes still suffer from severe microcracks generated by repeated planar gliding during cycling,which poses a great challenge to the cycling stability of single-crystal materials.It's worth noting that the microstructure of the cathode material is mainly inherited from its precursor.Therefore,it is necessary to deeply understand the influence of the microstructure of Ni_(1-x-y)Co_(x)M_(y)(OH)2 on the electrochemical properties of LiNi_(1-x-y)Co_(x)M_(y)O_(2) cathode materials,so as to optimize the production process of preparing high-performance cathode precursors.In this review,we summarize recent advances in the research and development of Ni-rich cathode precursor materials.Firstly,the challenges faced by the Ni-rich hydroxide precursor materials are presented,including the effect of primary particle morphology and arrangement on the electrochemical performance of cathode materials,the influence of secondary particle morphology on lithium insertion reactions in cathode,and the effect of particle size on the microcracking of single-crystal particles.Secondly,the presentation of the conventional co-precipitation reactor,the mechanism of precursor particle growth,and the influence of coprecipitation parameters are described in detail.Finally,the strategies are systematically discussed to solve the challenges of hydroxide precursors,such as the innovation and optimization on reactants,synthesis processes,and reaction equipment.To obtain satisfactory high-quality precursor materials,future work will require an in-depth understanding of the reaction mechanism,combined with simulation techniques such as flow field theory calculations to guide the synthesis of precursors.This review provides a comprehensive analysis of the current progresses on the producing technologies of highperformance cathode precursors and offers prospects for future industry developments. | Wenbiao Liang Yin Zhao Liyi Shi Zhuyi Wang Yi Wang Meihong Zhang Shuai Yuan | 2024 | Particuology2024，86，3： | 1 |
| 4 | Granularity control enables high stability and elevated-temperature properties of micron-sized single-crystal LiNi_(0.5)Mn_(1.5)O_(4) cathodes at high voltage显示文摘The development of high energy density LiNi_(0.5)Mn_(1.5)O_(4)(LNMO)cathode materials for lithium-ion batteries are challenged by capacity degradation,which becomes more aggravated particularly at elevated temperatures.Thus,the practical strategy with facile craft and the viability of large-scale preparation for industrialized applications should be developed urgently.In this work,a micron-sized LNMO single crystals is synthesized by a facile two-step method consisting of an alcohol gel solvent method and a segmented sintering reaction.Results show that the truncated polyhedron LNMO-900 sample,with the moderate D50 characteristic value of 4.429 mm and the highest tap density of 2.31 g cm^(-3),provides a stable structural and chemical stability even at elevated testing temperature due to its moderate specific surface area and the few Fd-3m phase.The LNMO/Li half-cells display more excellent capacity retention(87.3% at 1C and 25℃ after 500 cycles)and better thermal stability(76.65% at 1C and 55℃ after 200 cycles)than those of the single crystals of LNMO-850 and LNMO-950.Besides,the XPS,in-situ EIS and electrochemical tests results also prove that the LNMO-900 exhibits the lowest electrolyte decomposition degree,owing to a thin and effective solid-electrolyte interfacial film formed after cycles. | Wenbiao Liang Peng Wang Hao Ding Bo Wang Shiyou Li | 2021 | Journal of Materiomics2021，7，5： | 1 |
| 5 | Dielectric and Tunable Characteristics of Barium Strontium Titanate Modified with Al2O3 Addition显示文摘 | Liang Xiaofeng Wu Wenbiao Meng Zhongyan | 2003 | Materials Science and Engineering B2003，99，4： | 1 |
| 6 | Electron-ion collider in China显示文摘Lepton scattering is an established ideal tool for studying inner structure of small particles such as nucleons as well as nuclei.As a future high energy nuclear physics project,an Electron-ion collider in China(EicC)has been proposed.It will be constructed based on an upgraded heavy-ion accelerator,High Intensity heavy-ion Accelerator Facility(HIAF)which is currently under construction,together with a new electron ring.The proposed collider will provide highly polarized electrons(with a po-larization of 80%)and protons(with a polarization of 70%)with variable center of mass energies from 15 to 20 GeV and the luminosity of(2–3)×1033 cm^(−2)·s^(−1).Polarized deuterons and Helium-3,as well as unpolarized ion beams from Carbon to Uranium,will be also available at the EicC.The main foci of the EicC will be precision measurements of the structure of the nucleon in the sea quark region,including 3D tomography of nucleon;the partonic structure of nuclei and the parton interaction with the nuclear environment;the exotic states,especially those with heavy flavor quark contents.In addition,issues fundamental to understanding the origin of mass could be addressed by measurements of heavy quarkonia near-threshold production at the EicC.In order to achieve the above-mentioned physics goals,a hermetical detector system will be constructed with cutting-edge technologies.This document is the result of collective contributions and valuable inputs from experts across the globe.The EicC physics program complements the ongoing scientific programs at the Jefferson Laboratory and the future EIC project in the United States.The success of this project will also advance both nuclear and particle physics as well as accelerator and detector technology in China. | Daniele PAnderle Valerio Bertone Xu Cao Lei Chang Ningbo Chang Gu Chen Xurong Chen Zhuojun Chen Zhufang Cui Lingyun Dai Weitian Deng Minghui Ding Xu Feng Chang Gong Longcheng Gui Feng-Kun Guo Chengdong Han Jun He Tie-Jiun Hou Hongxia Huang Yin Huang KrešImir KumeričKi LPKaptari Demin Li Hengne Li Minxiang Li Xueqian Li Yutie Liang Zuotang Liang Chen Liu Chuan Liu Guoming Liu Jie Liu Liuming Liu Xiang Liu Tianbo Liu Xiaofeng Luo Zhun Lyu Boqiang Ma Fu Ma Jianping Ma Yugang Ma Lijun Mao Cédric Mezrag HervéMoutarde Jialun Ping Sixue Qin Hang Ren Craig DRoberts Juan Rojo Guodong Shen Chao Shi Qintao Song Hao Sun PawełSznajder Enke Wang Fan Wang Qian Wang Rong Wang Ruiru Wang Taofeng Wang Wei Wang Xiaoyu Wang Xiaoyun Wang Jiajun Wu Xinggang Wu Lei Xia Bowen Xiao Guoqing Xiao Ju-Jun Xie Yaping Xie Hongxi Xing Hushan Xu Nu Xu Shusheng Xu Mengshi Yan Wenbiao Yan Wencheng Yan Xinhu Yan Jiancheng Yang Yi-Bo Yang Zhi Yang Deliang Yao Zhihong Ye Peilin Yin C-PYuan Wenlong Zhan Jianhui Zhang Jinlong Zhang Pengming Zhang Yifei Zhang Chao-Hsi Chang Zhenyu Zhang Hongwei Zhao Kuang-Ta Chao Qiang Zhao Yuxiang Zhao Zhengguo Zhao Liang Zheng Jian Zhou Xiang Zhou Xiaorong Zhou Bingsong Zou Liping Zou | 2021 | Frontiers of physics2021，16，6： | 1 |
| 7 | 显示文摘 | Liang Xiaofeng Wu Wenbiao Meng Yan | 2003 | Chinese Journal of Rare Metals2003，99，： | 1 |
| 8 | SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication显示文摘Viral infection in respiratory tract usually leads to cell death,impairing respiratory function to cause severe disease.However,the diversity of clinical manifestations of SARS-CoV-2 infection increases the complexity and difficulty of viral infection prevention,and especially the high-frequency asymptomatic infection increases the risk of virus transmission.Studying how SARS-CoV-2 affects apoptotic pathway may help to understand the pathological process of its infection.Here,we uncovered SARS-CoV-2 imployed a distinct anti-apoptotic mechanism via its N protein.We found SARS-CoV-2 virus-like particles(trVLP)suppressed cell apoptosis,but the trVLP lacking N protein didn’t.Further study verified that N protein repressed cell apoptosis in cultured cells,human lung organoids and mice.Mechanistically,N protein specifically interacted with anti-apoptotic protein MCL-1,and recruited a deubiquitinating enzyme USP15 to remove the K63-linked ubiquitination of MCL-1,which stabilized this protein and promoted it to hijack Bak in mitochondria.Importantly,N protein promoted the replications of IAV,DENV and ZIKV,and exacerbated death of IAV-infected mice,all of which could be blocked by a MCL-1 specific inhibitor,S63845.Altogether,we identifed a distinct anti-apoptotic function of the N protein,through which it promoted viral replication.These may explain how SARS-CoV-2 effectively replicates in asymptomatic individuals without cuasing respiratory dysfunction,and indicate a risk of enhanced coinfection with other viruses.We anticipate that abrogating the N/MCL-1-dominated apoptosis repression is conducive to the treatments of SARS-CoV-2 infection as well as coinfections with other viruses. | Pan Pan Weiwei Ge Zhiwei Lei Wei luo Yuqing Liu Zhanwen Guan Lumiao Chen Zhenyang Yu Miaomiao Shen Dingwen Hu Qi Xiang Wenbiao Wang Pin Wan Mingfu Tian Yang Yu Zhen Luo Xulin Chen Heng Xiao Qiwei Zhang Xujing Liang Xin Chen Yongkui Li Jianguo Wu | 2023 | Signal Transduction and Targeted Therapy2023，8，6： | 0 |
| 9 | ARTICLE Research on the Leading Value Drive of Rural Homestead Transfer under Rural Revitalization--Based on the Evidences of China显示文摘With the development of urban-rural integration in China,the functional value of homestead bases has evolved from a single residential security value to a multiple composite values,and the property income of homestead bases has gradually become the value driver of transfer and the intrinsic demand of farm households.This paper takes Baitafan of Jinzhai County,Chongqing City,and Xiaofang Yu Village of Ji County as examples for in-depth discussion,and finds that the dominant value drivers of home base transfer mainly include three kinds:capitalization income,commercialization income,and non-farm employment income.The study concludes that it is important to give full play to the resource endowment effect and identify the dominant value of home base transfer according to local conditions to promote the standardized home base transfer and implement the rural revitalization strategy. | He Huang Ailun Liang Shiqi Fang Jiexuan Chen Wenbiao Li Yuyang Ma Yuchun Wang Chien Chi Chu | 2022 | Journal of Economic Science Research2022，5，1： | 0 |
| 10 | SARS-CoV-2 N protein induces acute kidney injury in diabetic mice via the Smad3-Ripk3/MLKL necroptosis pathway显示文摘Dear Editor,Kidney is one of major organs attacked by SARS-CoV-2,resulting in acute kidney injury(AKI)in critically ill COVID-19 patients,especially in the elderly and diabetic patients with diabetic kidney disease(DKD).1,2 Among SARS-CoV-2 proteins,the N protein can be detectable in damaged tubules in COVID-19 patients with AKI. | Liying Liang Wenbiao Wang Junzhe Chen Wenjing Wu Xiao-Ru Huang Biao Wei Yu Zhong Ronald C.W.Ma Xueqing Yu Hui-Yao Lan | 2023 | Signal Transduction and Targeted Therapy2023，8，5： | 0 |
| 11 | Preparation of PVA@PEI@BAC@CNC composite nanofibrous film with high efficiency filtration for PM2.5显示文摘It is still a challenge to prepare a water-and polymer-based electrospun air filter film with high efficiency filtration,low pressure drop,and good mechanical properties.To address this issue,polyvinyl alcohol(PVA)was employed as the main material,mixing polyethyleneimine(PEI),bamboo-based activated carbon(BAC)and cellulose nanocrystal(CNC)to construct the air filter film by electrostatic electrospinning.In this system,the negatively charged BAC and CNC are fixed in the system through bonding with the positively charged PEI,showing a double adsorption effect.One is the mechanical filtration of the porous network structure constructed by PVA@PEI electrospun nanofibers,and the other is the electrostatic adsorption of PM2.5 on the surface of BAC and CNC.It is significant that the resulting composite air filter displays a high filtration efficiency of 95.86%,a pressure drop of only 59 Pa,and good thermal stability.Moreover,the introduced methyltrimethoxysilane(MTMS)endows it with good water-resistance.Given these excellent performances,this system can provide theoretical and technical references for the development of water-and polymerbased electrospun air filter film. | Jingda Huang Yi Wang Yuxin Cai Yipeng Liang Shite Lin Enfu Wang Jinhuan Zhong Wenbiao Zhang Kuichuan Sheng | 2023 | Frontiers of Materials Science2023，17，4： | 0 |
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