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| 1 | African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2显示文摘African swine fever virus(ASFV),as a member of the large DNA viruses,may regulate autophagy and apoptosis by inhibiting programmed cell death.However,the function of ASFV proteins has not been fully elucidated,especially the role of autophagy in ASFV infection.One of three Pyrroline-5-carboxylate reductases(PYCR),is primarily involved in conversion of glutamate to proline.Previous studies have shown that depletion of PYCR2 was related to the induction of autophagy.In the present study,we found for the first time that ASFV E199 L protein induced a complete autophagy process in Vero and HEK-293 T cells.Through co-immunoprecipitation coupled with mass spectrometry(Co IP-MS)analysis,we firstly identified that E199 L interact with PYCR2 in vitro.Importantly,our work provides evidence that E199 L down-regulated the expression of PYCR2,resulting in autophagy activation.Overall,our results demonstrate that ASFV E199 L protein induces complete autophagy through interaction with PYCR2 and down-regulate the expression level of PYCR2,which provide a valuable reference for the role of autophagy during ASFV infection and contribute to the functional clues of PYCR2. | Sheng Chen Xinheng Zhang Yu Nie Hongxin Li Weiguo Chen Wencheng Lin Feng Chen Qingmei Xie | 2021 | Virologica Sinica2021，36，2： | 7 |
| 2 | Synergistic effects of ginseng stem and leaf-extracted ginsenoside and choline on improving learning and memory in rats Association verification experiment in animals with multiple learning and memory disorders显示文摘BACKGROUND: Ginsenoside extracted from the stem and leaf of ginseng (GSL) and choline have both been shown to improve learning and memory functions; however,further studies are needed to understand the synergistic effects of a combination of both. OBJECTIVE: To verify the combined improved synergistic effects of GSL and choline on learning and memory disorders in rats. DESIGN: Control observation. SETTING: Taishan Medical College. MATERIALS: A total of 150 male Kunming mice weighing (20±2) g and 40 healthy male Wistar rats weighing (220±20) g were provided by the Experimental Animal Department of Jilin University. Animal experimentation received confirmed consent from the local ethic committee. GSL was provided by the Department of Chemistry,Norman Bethune Medical University,and choline was provided by the Third Experiment Factory,Shanghai. METHODS: This study was performed at the Life Science Institute,Taishan Medical College from October 2006 to February 2007. ① Scopolamine-induced learning and memory disorders in rats: Forty rats were randomly divided into control group,model group,combination group (400 mg/kg GSL + 200 mg/kg choline),GSL (400 mg/kg) group,and choline (200 mg/kg) group,8 rats/group. Rats were perfused and administrated in the morning,once a day for 14 successive days. Rats in the control group and model group were perfused with 20 mL/kg distilled water and underwent Morris water maze spatial resolution test 1 hour after perfusion on the 10th,11th,and 12th days after administration. Rats also underwent passive step-down avoidance test 1 hour after reperfusion on the 13th and 14th days after administration. Thirty minutes prior to experimentation,rats in the remaining three groups were intraperitoneally (i.p) injected with 2 mg/kg scopolamine,and rats in the control group were i.p. injected with 2 mL/kg saline. ② Scopolamine-induced learning disorder and memory acquired disorder in mice: Fifty mice were randomly divided into control group,model group,combination group (400 mg/kg GSL +200 mg/kg choline),GSL (400 mg/kg) group,and choline (200 mg/kg) group,with 10 mice/group. Mice were perfused and administrated in the morning,once a day for 9 successive days. Mice in the control group and model group were perfused with 20 mL/kg distilled water and underwent passive step down avoidance test 1 hour after reperfusion on the 8th and 9th day after administration. Twenty minutes prior to training,mice in the remaining three groups were i.p. injected with 2 mg/kg scopolamine,and mice in the control group were i.p. injected with 10 mL/kg saline. ③ Sodium nitrite-induced memory consolidation disorder in mice: Grouping,administration,and testing were the same as mentioned above. After training,mice in the remaining three groups were immediately subcutaneously injected with 120 mg/kg sodium nitrite,and mice in the control group were subcutaneously injected with 20 mL/kg saline. ④ Ethanol-induced memory reconsolidation disorder in mice: Grouping,administration,and testing were the same as mentioned above. At 24 hours after training and 20 minutes before retraining,mice in the remaining four groups were perfused with 10 mL/kg ethanol (0.3 volume fraction),and mice in the control group were perfused with 10 mL/kg saline. MAIN OUTCOME MEASURES: Synergistic effects of GSL and choline on learning and memory deficits induced by scopolamine,sodium nitrite,and ethanol in experimental animals. RESULTS: All 40 rats and 150 mice were included in the final analysis. ① Synergistic effects of GSL and choline on learning and memory disorders induced by scopolamine in rats: During passive step-down avoidance and Morris water maze spatial resolution tests,the number of error responses and length of maze training in the model group were significantly greater than in the control group (P < 0.01); while the number of error responses and length of maze training in the combination group were significantly less than in the model group,GSL group,and choline group (P < 0.05-0.01). The Q value was > 1 after combining administration,which suggests that the combination of GSL and choline had synergistic effects. ② Synergistic effects of GSL and choline on learning disorder and memory-acquired disorder induced by scopolamine in mice: During passive step-down avoidance test,the number of error responses in the model group were significantly greater than in the control group (P < 0.01); while the number of error responses in the combination group were significantly less than in the model group,GSL group,and choline group (P < 0.05-0.01). The Q value was > 1 after combining administration,which suggests GSL and choline had synergistic effects. ③ Synergistic effects of GSL and choline on memory sodium nitrate-induced consolidation disorder in mice: During passive step down avoidance test,the number of error responses in the model group were significantly less than in the control group (P < 0.01); while the number of error responses in the combination group were significantly less than in the model group,GSL group,and choline group (P < 0.05-0.01). The Q value was > 1 after combined administration,which suggests GSL and choline had synergistic effects. ④ Synergistic effects of GSL and choline on ethanol-induced memory reconsolidation disorder in mice: During passive step down avoidance test,the number of error responses in the model group were significantly greater than in the control group (P < 0.01); while the number of error responses in the combination group were significantly less than in the model group,GSL group,and choline group (P < 0.05-0.01). The Q value was > 1 after combined administration,which suggests GSL and choline had synergistic effects. CONCLUSION: GSL and choline have synergistic effects on learning and memory functions. | Xiaomin Zhao Hongxia GU Qing Li Xianglin Xie Zuoli Xia Hongxin Cai Ling Zhang Dawei Li Xinnong Wang | 2008 | Neural Regeneration Research2008，3，2： | 3 |
| 3 | A novel cancer vaccine with the ability to simultaneously produce anti-PD-1 antibody and GM-CSF in cancer cells and enhance Th1-biased antitumor immunity显示文摘Tumor escape from immune-mediated destruction has been associated with immunosuppressive mechanisms that inhibit T-cell activation.A promising strategy for cancer immunotherapy is to disrupt key pathways regulating immune tolerance,such as program death-1(PD-1/PD-L1)pathway in the tumor environment.However,the determinants of response to anti-PD-1 monoclonal antibodies(mAbs)treatment remain incompletely understood.In murine models,PD-1 blockade alone fails to induce effective immune responses to poorly immunogenic tumors,but is successful when combined with additional interventions,such as cancer vaccines.Novel cancer vaccines combined with antibody may offer promising control of cancer development and progression.In this investigation,we generated a novel tumor cell vaccine simultaneously expressing anti-PD-1 mAbs and granulocyte-macrophage colony stimulating factor(GM-CSF)in CT26 colon cancer and B16-F10 melanoma.The antitumor effect of the vaccine was verified by therapeutic and adoptive animal experiments in vivo.The antitumor mechanism was analyzed using Flow cytometry,Elispot and in vivo intervention approaches.The results showed that tumor cell vaccine secreting PD-1 neutralizing antibodies and GM-CSF induced remarkable antitumor immune effects and prolonged the survival of tumor-bearing animals compared with animals treated with either PD-1 mAbs or GM-CSF alone.Antitumor effects and prolonged survival correlated with strong antigen-specific T-cell responses by analyzing CD11c^(+)CD86^(+)DC,CD11b^(+)F4/80^(+)MΦcells,increased ratio of Teff/Treg in the tumor microenvironment,and higher secretion levels of Th1 proinflammatory cytokines in serum.Furthermore,the results of ELISPOT and in vivo blocking strategies further confirmed that the antitumor immune response is acquired by CD4 and CD8 T immune responses,primarily dependent on CD4 Th1 immune response,not NK innate immune response.The combination of PD-1 blockade with GM-CSF secretion potency creates a novel tumor cell vaccine immunotherapy,affording significantly improved antitumor responses by releasing the state of immunosuppressive microenvironment and augmenting the tumor-reactive T-cell responses. | Hongwei Tian Gang Shi Qin Wang Yiming Li Qianmei Yang Chunlei Li Guoyou Yang Min Wu Qian Xie Shuang Zhang Yang Yang Rong Xiang Dechao Yu Yuquan Wei Hongxin Deng | 2016 | Signal Transduction and Targeted Therapy2016，1，1： | 3 |
| 4 | Two Ultraviolet Radiation Datasets that Cover China显示文摘Ultraviolet(UV) radiation has significant effects on ecosystems, environments, and human health, as well as atmospheric processes and climate change. Two ultraviolet radiation datasets are described in this paper. One contains hourly observations of UV radiation measured at 40 Chinese Ecosystem Research Network stations from 2005 to 2015. CUV3 broadband radiometers were used to observe the UV radiation, with an accuracy of 5%, which meets the World Meteorology Organization's measurement standards. The extremum method was used to control the quality of the measured datasets. The other dataset contains daily cumulative UV radiation estimates that were calculated using an all-sky estimation model combined with a hybrid model. The reconstructed daily UV radiation data span from 1961 to 2014. The mean absolute bias error and root-mean-square error are smaller than 30% at most stations, and most of the mean bias error values are negative, which indicates underestimation of the UV radiation intensity. These datasets can improve our basic knowledge of the spatial and temporal variations in UV radiation. Additionally, these datasets can be used in studies of potential ozone formation and atmospheric oxidation, as well as simulations of ecological processes. | Hui LIU Bo HU Yuesi WANG Guangren LIU Liqin TANG Dongsheng JI Yongfei BAI Weikai BAO Xin CHEN Yunming CHEN Weixin DING Xiaozeng HAN Fei HE Hui HUANG Zhenying HUANG Xinrong LI Yan LI Wenzhao LIU Luxiang LIN Zhu OUYANG Boqiang QIN Weijun SHEN Yanjun SHEN Hongxin SU Changchun SONG Bo SUN Song SUN Anzhi WANG Genxu WANG Huimin WANG Silong WANG Youshao WANG Wenxue WEI Ping XIE Zongqiang XIE Xiaoyuan YAN Fanjiang ZENG Fawei ZHANG Yangjian ZHANG Yiping ZHANG Chengyi ZHAO Wenzhi ZHAO Xueyong ZHAO Guoyi ZHOU Bo ZHU | 2017 | Advances in Atmospheric Sciences2017，34，7： | 2 |
| 5 | A novel injectable local hydrophobic drug delivery system: Biodegradable nanoparticles in thermo-sensitive hydrogel显示文摘 | MaLing Gou XingYi Li Mei Dai ChangYang Gong XianHuo Wang Yao Xie HongXin Deng LiJuan Chen Xia Zhao ZhiYong Qian YuQuan Wei | 2008 | International Journal of Pharmaceutics2008，，1： | 1 |
| 6 | Characteristic modeling and the control of flexible structure显示文摘 | Hongxin Wu Yiwu Liu Zhonghan Liu Yongchun Xie | 2001 | Science in China Series : Information Sciences2001，，4： | 1 |
| 7 | Advances in identification and application of tumor antigen inducing anti-cancer responses显示文摘 | XIE Ke TIAN Ling DENG Hongxin WEI Yuquan | 2003 | Chinese Science Bulletin2003，48，11： | 1 |
| 8 | Characteristic modeling and the control of flexible structure显示文摘 | Hongxin Wu Yiwu Liu Zhonghan Liu Yongchun Xie | 2001 | Science in China Series : Information Sciences2001，，4： | 1 |
| 9 | Characteristicmodel-based all-coefficient adaptive control method andits applications显示文摘 | Wu Hongxin Hu Jun Xie Yongchun | 2007 | IEEE Trans on Systems Man andCybernetics Part C: Applications and Reviews2007，37，2： | 1 |
| 10 | Characteristic modeling and the control of flexible structure显示文摘 | Hongxin Wu Yiwu Liu Zhonghan Liu Yongchun Xie | 2001 | Science in China Series : Information Sciences2001，，4： | 1 |
| 11 | Characteristic Model-Based All-coefficient Adaptive Control Method and Its Applications显示文摘 | Wu Hongxin Hu Jun Xie Yongchun | 2007 | IEEE Trans on Systems Man and Cybernetics-Part C:Applications and Reviews2007，37，2： | 1 |
| 12 | Characteristic model-based all-coefficient adaptive control method and its applications 显示文摘 | Wu Hongxin Hu Jun Xie Yongchun | 2007 | IEEE Transactions on Systems Man and Cybernetics2007，37，2： | 1 |
| 13 | ADVANCES IN NETWORK FUNCTION VIRTUALIZATION显示文摘In traditional networks,enabling new network functions often needs to add new proprietary middleboxes.However,finding the space and power to accommodate these middleboxes is becoming increasingly difficult, | Jun Bi Gaogang Xie Hongxin Hu | 2018 | China Communications2018，15，10： | 0 |
| 14 | Field Surveys Suggest Biennial Reproduction Cycle and Competition-triggered Dispersal of the Endangered Chinese Crocodile Lizard显示文摘The endangered Chinese crocodile lizard(Shinisaurus crocodilurus)is a habitat specialist living in streams of mountain forests in southern China and northern Vietnam.Conservation efforts are increasing for recovering its wild populations.However,the growth,reproduction,and dispersal ability of crocodile lizards in the wild are largely unknown.We conducted field surveys of the crocodile lizard population in Daguishan National Nature Reserve,one of the largest extant wild populations of crocodile lizards,for three consecutive years in Guangxi,China.We found that crocodile lizards generally reach sexual maturity at the age of 2.5 years in the wild.Unlike most viviparous lizards,which reproduce annually,the crocodile lizard shows a biennial reproductive cycle.The number of observed juveniles and subadults fluctuated between years,whereas that of adults remained relatively stable.Non-adults had longer three-year dispersal distance than adults.Crocodile lizards showed preference for backwater pools in the stream.Competition for better resources may be the main trigger for dispersal. | Shuyi LUO Yujie YANG Chunsheng YANG Jun GUO Xudong QIN Haiyao CEN Hongxin XIE Zhengjun WU | 2022 | Asian Herpetological Research2022，13，3： | 0 |
| 15 | Deep Segmentation Feature-Based Radiomics Improves Recurrence Prediction of Hepatocellular Carcinoma显示文摘Objective and Impact Statement.This study developed and validated a deep semantic segmentation feature-based radiomics(DSFR)model based on preoperative contrast-enhanced computed tomography(CECT)combined with clinical information to predict early recurrence(ER)of single hepatocellular carcinoma(HCC)after curative resection.ER prediction is of great significance to the therapeutic decision-making and surveillance strategy of HCC.Introduction.ER prediction is important for HCC.However,it cannot currently be adequately determined.Methods.Totally,208 patients with single HCC after curative resection were retrospectively recruited into a model-development cohort(n=180)and an independent validation cohort(n=28).DSFR models based on different CT phases were developed.The optimal DSFR model was incorporated with clinical information to establish a DSFR-C model.An integrated nomogram based on the Cox regression was established.The DSFR signature was used to stratify high-and low-risk ER groups.Results.A portal phase-based DSFR model was selected as the optimal model(area under receiver operating characteristic curve(AUC):development cohort,0.740;validation cohort,0.717).The DSFR-C model achieved AUCs of 0.782 and 0.744 in the development and validation cohorts,respectively.In the development and validation cohorts,the integrated nomogram achieved C-index of 0.748 and 0.741 and time-dependent AUCs of 0.823 and 0.822,respectively,for recurrence-free survival(RFS)prediction.The RFS difference between the risk groups was statistically significant(P<0.0001 and P=0.045 in the development and validation cohorts,respectively).Conclusion.CECT-based DSFR can predict ER in single HCC after curative resection,and its combination with clinical information further improved the performance for ER prediction. | Jifei Wang Dasheng Wu Meili Sun Zhenpeng Peng Yingyu Lin Hongxin Lin Jiazhao Chen Tingyu Long Zi-Ping Li Chuanmiao Xie Bingsheng Huang Shi-Ting Feng | 2022 | Biomedical Engineering Frontiers2022，3，1： | 0 |
| 16 | Corrigendum to“African swine fever virus protein E199L promotes cell autophagy through the interaction of PYCR2”[Virol Sin 36(2021)196–206]显示文摘Due to our negligence,the original version of this article,published online on April 08,2021,contained a mistake in Fig.1E.The lane ofβ-actin in Western blotting was misused.The correct Fig.1 is given below.We apologize for our oversight when preparing the figure and state that this does not change the scientific conclusions of the article in any way. | Sheng Chen Xinheng Zhang Yu Nie Hongxin Li Weiguo Chen Wencheng Lin Feng Chen Qingmei Xie | 2023 | Virologica Sinica2023，38，3： | 0 |
| 17 | 三阴性乳腺癌在新辅助化疗中获得病理完全缓解率的Meta分析(英文)显示文摘Objective:The pathological complete response(pCR) rates of neoadjuvant chemotherapy(NAC) in triple-negative breast cancer(TNBC) was reported higher than that in non-TNBC but ranged from 12% to 48%. pCR was reported to be a predictor of long overall survival and exact pCR rate of NAC in TNBC would give us some hints on how to improve outcomes of TNBC patients. The meta-analysis was conducted to estimate the pCR rate of NAC for TNBC through contrasting the pCR rates of TNBC and non-TNBC tumors in NAC. Methods: Studies were selected from the PubMed database and Cochrane Collaboration Library. pCR rates were collected in groups of TNBC and non-TNBC tumors. Review Manager 4.2 was used to perform forest plots and funnel plots. Results: The analysis included 22 studies with 7168 patients, the aggregate pCR rate was 29.5% in TNBC group, which was 17.7% higher than non-TNBC. The summary relative risk(RR) for pCR rate of TNBC group with that of non-TNBC group was 2.55. No obvious statistical heterogeneity and publication bias was detected. Conclusion: This meta-analysis demonstrated that NAC showed a higher pCR rate in TNBC than non-TNBC. | Guojing Zhang Wanqing Xie Chao Lin Zhaozhe Liu Long Xu Guanzhong Zhang Fang Guo Yaling Han Hongxin Zheng Xiaodong Xie | 2014 | The Chinese-German Journal of Clinical Oncology2014，13，2： | 0 |
| 18 | Evaluation of the nonmarket value of livestock and poultry feces returning to farmland utilization using CVM in Heilongjiang,China显示文摘Aiming at a series of hot issues and topics,such as resource waste,environmental pollution,and Mollisol protection,related to the arbitrary discharge of livestock and poultry feces,livestock and poultry feces are taken as the research object,a questionnaire survey was conducted to investigate residents’cognition and willingness to pay(WTP)for the nonmarket value of livestock and poultry feces returning to farmland in Heilongjiang Province,China.The contingent valuation method(CVM)was used for the correlation analysis of WTP and socioeconomic characteristics,and correlation analysis of payment mode and socioeconomic characteristics.Meanwhile,the factors influencing WTP and payment mode were analyzed.The results show that the majority of the respondents are aware of the harmfulness of the arbitrary discharge of livestock and poultry feces and the importance of its nonmarket regeneration value.Approximately 90.09% of respondents were willing to pay for the return of livestock and poultry feces to fields.In 2020,the total value of WTP of livestock and poultry feces returning to farmland in Heilongjiang Province was 4.65 billion CNY,equivalent to 1.32% of the total agricultural production of Heilongjiang Province in the same year.And the nonmarket value of livestock and poultry feces is estimated to be 1456.69 CNY/t.The annual average WTP of rural and urban households is 286.42 CNY and 422.90 CNY per household,accounting for 0.46% and 0.52% of the average annual income of households,respectively.Therefore,the return of livestock and poultry feces to farmland utilization has a high nonmarket value.The analysis shows that the average annual household income is a significant factor in WTP,and the registered permanent residence type and the average annual household income are significant factors in payment mode.The research results should provide a scientific and theoretical basis for the government’s policy and decision-making on the return of livestock and poultry feces to farmland. | Jiajie Shang Yongtao Xie Lifeng Guo Jinxia Fan Hongxin Liu | 2023 | International Journal of Agricultural and Biological Engineering2023，16，2： | 0 |
| 19 | Author Correction: A novel cancer vaccine with the ability to simultaneously produce anti-PD-1 antibody and GM-CSF in cancer cells and enhance Th1-biased antitumor immunity显示文摘Correction to:Signal Transduction and Targeted Therapy(2016)1:16025 http://gffzzd3cc09b8251d45dfspn5uxf5op50p656u.ffgz.tsg.suse.edu.cn/10.1038/sigtrans.2016.25,published online 18 November 2016 Since the publication of this research Article,we retrospected the paper and noticed two inadvertent mistakes that need to be corrected immediately.We have checked the original data and repeated the experiments;the correct data are provided in this Corrigendum as follows.The key findings of the article are not affected by these corrections. | Hongwei Tian Gang Shi Qin Wang Yiming Li Qianmei Yang Chunlei Li Guoyou Yang Min Wu Qian Xie Shuang Zhang Yang Yang Rong Xiang Dechao Yu Yuquan Wei Hongxin Deng | 2019 | Signal Transduction and Targeted Therapy2019，4，1： | 0 |
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