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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Anti-malaria drug chloroquine is highly effective in treating avian influenza A H5N1 virus infection in an animal model显示文摘 | Yiwu Yan Zhen Zou Yang Sun Xiao Li Kai-Feng Xu Yuquan Wei Ningyi Jin Chengyu Jiang | 2013 | Cell Research2013，23，2： | 36 |
| 3 | 5-Hydroxymethylcytosine signatures in cell-free DNA provide information about tumor types and stages显示文摘5-Hydroxymethylcytosine (5hmC ) 是被连接了到基因规定和癌症致病的重要哺乳动物的 DNA epigenetic 修正。这里,我们用定序途径的一枝敏感化学基于标签的低输入的猎枪在传播没有房间的 DNA (cfDNA ) 探索了 5hmC 的诊断潜力。我们从七种不同癌症类型的 49 个病人定序没有房间的 5hmC 并且发现了能被用来与高精确性预言癌症类型和阶段的不同特征。明确地,我们发现肺癌症在 cfDNA 导致 5hmC 的进步全球损失,而 hepatocellular 癌和胰腺的癌症在没有房间的 hydroxymethylome 导致疾病特定的变化。我们的 proof-of-principle 结果建议没有房间的 5hmC 签名可以潜在地被使用不仅识别癌症类型而且在一些癌症追踪肿瘤舞台。 | Chun-Xiao Song Senlin Yin Li Ma Amanda Wheeler Yu Chen Yan Zhang Bin Liu Junjie Xiong Weihan Zhang Jiankun Hu Zongguang Zhou Biao Dong Zhiqi Tian Stefanie S Jeffrey Mei-Sze Chua Samuel So Weimin Li Yuquan Wei Jiajie Diao Dan Xie Stephen R Quake | 2017 | Cell Research2017，27，10： | 17 |
| 4 | SHRIMP zircon U-Pb dating for gabbro from the Tiding ophiolite in Tibet显示文摘 | WANG Ran XIA Bin ZHOU Guoqing ZHANG Yuquan YANG Zhiqing LI Wenqian WEI Dongliang ZHONG Lifeng XU Lifeng | 2006 | Chinese Science Bulletin2006，51，14： | 14 |
| 5 | CCL5/CCR5 axis in human diseases and related treatments显示文摘To defense harmful stimuli or maintain the immune homeostasis, the body produces and recruits a superfamily of cytokines such as interleukins, interferons, chemokines etc. Among them, chemokines act as crucial regulators in defense systems. CCL5/CCR5 combination is known for facilitating inflammatory responses, as well as inducing the adhesion and migration of different T cell subsets in immune responses. In addition, recent studies have shown that the interaction between CCL5 and CCR5 is involved in various pathological processes including inflammation, chronic diseases, cancers as well as the infection of COVID-19. This review focuses on how CCL5/CCR5 axis participates in the pathological processes of different diseases and their relevant signaling pathways for the regulation of the axis. Moreover, we highlighted the gene therapy and chemotherapy studies for treating CCR5-related diseases, including the ongoing clinical trials. The barriers and perspectives for future application and translational research were also summarized. | Zhen Zeng Tianxia Lan Yuquan Wei Xiawei Wei | 2022 | Genes & Diseases2022，9，1： | 12 |
| 6 | Mitochondrial DNA in the regulation of innate immune responses显示文摘线粒体作为房间的精力工厂被知道,它也是一个唯一的哺乳动物的细胞器并且考虑超过 10 亿年以前从氧气的初核质被发展。Mitochondrial DNA,类似于它的细菌的祖先,由一个圆形的环组成并且作为 CpG 岛包含 unmethylated DNA 的重要数字。天生的免疫系统在哺乳动物的有免疫力的反应起一个重要作用。最近的研究证明了 mitochondrial DNA (mtDNA ) 激活包含发信号的 TLR9, NLRP3 和圈套的几条天生的有免疫力的小径,它在受动器回答贡献发信号的平台和结果。除了便于抗菌剂豁免并且调整抗病毒的发信号,装证据建议 mtDNA 贡献跟随细胞的损坏和应力的煽动性的疾病。除了它在细胞的新陈代谢和精力生产的欣赏得好的角色,因此, mtDNA 看起来在天生的免疫系统作为一个关键成员工作。这里,我们在天生的免疫加亮 mtDNA 的新兴的角色。 | Chunju Fang Xiawei Wei Yuquan Wei | 2016 | Protein & Cell2016，7，1： | 12 |
| 7 | JMJD3 in the regulation of human diseases显示文摘In recent years,many studies have shown that histone methylation plays an important role in maintaining the active and silent state of gene expression in human diseases.The Jumonji domain-containing protein D3(JMJD3),specifically demethylate di-and trimethyllysine 27 on histone H3(H3K27me2/3),has been widely studied in immune diseases,infectious diseases,cancer,developmental diseases,and aging related diseases.We will focus on the recent advances of JMJD3 function in human diseases,and looks ahead to the future of JMJD3 gene research in this review. | Xiangxian Zhang Li Liu Xia Yuan Yuquan Wei Xiawei | 2019 | Protein & Cell2019，10，12： | 10 |
| 8 | Epigenetic regulation of macrophages: from homeostasis maintenance to host defense显示文摘Macrophages are crucial members of the innate immune response and important regulators.The differentiation and activation of macrophages require the timely regulation of gene expression,which depends on the interaction of a variety of factors,including transcription factors and epigenetic modifications.Epigenetic changes also give macrophages the ability to switch rapidly between cellular programs,indicating the ability of epigenetic mechanisms to affect phenotype plasticity.In this review,we focus on key epigenetic events associated with macrophage fate,highlighting events related to the maintenance of tissue homeostasis,responses to different stimuli and the formation of innate immune memory.Further understanding of the epigenetic regulation of macrophages will be helpful for maintaining tissue integrity,preventing chronic inflammatory diseases and developing therapies to enhance host defense. | Siyuan Chen Jing Yang Yuquan Wei Xiawei Wei | 2020 | Cellular & Molecular Immunology2020，17，1： | 8 |
| 9 | CRISPR/Cas9-mediated correction of human genetic disease显示文摘The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas) protein 9 system(CRISPR/Cas9) provides a powerful tool for targeted genetic editing. Directed by programmable sequence-specific RNAs,this system introduces cleavage and double-stranded breaks at target sites precisely. Compared to previously developed targeted nucleases, the CRISPR/Cas9 system demonstrates several promising advantages, including simplicity, high specificity,and efficiency. Several broad genome-editing studies with the CRISPR/Cas9 system in different species in vivo and ex vivo have indicated its strong potential, raising hopes for therapeutic genome editing in clinical settings. Taking advantage of non-homologous end-joining(NHEJ) and homology directed repair(HDR)-mediated DNA repair, several studies have recently reported the use of CRISPR/Cas9 to successfully correct disease-causing alleles ranging from single base mutations to large insertions. In this review, we summarize and discuss recent preclinical studies involving the CRISPR/Cas9-mediated correction of human genetic diseases. | Ke Men Xingmei Duan Zhiyao He Yang Yang Shaohua Yao Yuquan Wei | 2017 | Science China(Life Sciences)2017，60，5： | 7 |
| 10 | Molecular mechanisms and clinical management of cancer bone metastasis显示文摘As one of the most common metastatic sites of malignancies,bone has a unique microenvironment that allows metastatic tumor cells to grow and flourish.The fenestrated capillaries in the bone,bone matrix,and bone cells,including osteoblasts and osteoclasts,together maintain the homeostasis of the bone microenvironment.In contrast,tumor-derived factors act on bone components,leading to subsequent bone resorption or excessive bone formation.The various pathways involved also provide multiple targets for therapeutic strategies against bone metastases.In this review,we summarize the current understanding of the mechanism of bone metastases.Based on the general process of bone metastases,we specifically highlight the complex crosstalk between tumor cells and the bone microenvironment and the current management of cancer bone metastases. | Manni Wang Fan Xia Yuquan Wei Xiawei Wei | 2020 | Bone Research2020，8，3： | 7 |
| 11 | Multifunctional regulatory protein connective tissue growth factor (CTGF):A potential therapeutic target for diverse diseases显示文摘Connective tissue growth factor(CTGF),a multifunctional protein of the CCN family,regulates cell proliferation,differentiation,adhesion,and a variety of other biological processes.It is involved in the disease-related pathways such as the Hippo pathway,p53 and nuclear factor kappa-B(NF-κB)pathways and thus contributes to the developments of inflammation,fibrosis,cancer and other diseases as a downstream effector.Therefore,CTGF might be a potential therapeutic target for treating various diseases.In recent years,the research on the potential of CTGF in the treatment of diseases has also been paid more attention.Several drugs targeting CTGF(monoclonal antibodies FG3149 and FG3019)are being assessed by clinical or preclinical trials and have shown promising outcomes.In this review,the cellular events regulated by CTGF,and the relationships between CTGF and pathogenesis of diseases are systematically summarized.In addition,we highlight the current researches,focusing on the preclinical and clinical trials concerned with CTGF as the therapeutic target. | Minyang Fu Dandan Peng Tianxia Lan Yuquan Wei Xiawei Wei | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 5 |
| 12 | Immunotherapy of tumor by targeting angiogenesis显示文摘Tumor growth and metastasis are angiogenesis-dependent. Anti-angiogenic therapy represents a new strategy for the development of anti-cancer therapies. In recent years, there has been made great progress in anti-angiogenic therapy. As far as the passive immunotherapy is concerned, a recombinant humanized antibody to vascular endothelial growth factor (VEGF)— Avastin has been approved by FDA as the first angiogenesis inhibitor to treat colorectal cancer. For active specific immunotherapy, various strategies for cancer vaccines, including whole en- dothelial cell vaccines, dendritic cell vaccines, DNA vaccines, and peptides or protein vaccines, have been developed to break immune tolerance against important molecules associated with tumor angiogenesis and induce angiogenesis-specific immune responses. This article reviews the angiogenesis-targeted immunotherapy of tumor from the above two aspects. | HOU Jianmei TIAN Ling WEI Yuquan | 2004 | Science China(Life Sciences)2004，47，6： | 5 |
| 13 | Recent advances in lentiviral vectors for gene therapy显示文摘Lentiviral vectors(LVs), derived from human immunodeficiency virus, are powerful tools for modifying the genes of eukaryotic cells such as hematopoietic stem cells and neural cells. With the extensive and in-depth studies on this gene therapy vehicle over the past two decades, LVs have been widely used in both research and clinical trials. For instance, third-generation and selfinactive LVs have been used to introduce a gene with therapeutic potential into the host genome and achieve targeted delivery into specific tissue. When LVs are employed in leukemia, the transduced T cells recognize and kill the tumor B cells;in β-thalassemia, the transduced CD34^(+) cells express normal β-globin;in adenosine deaminase-deficient severe combined immunodeficiency, the autologous CD34^(+) cells express adenosine deaminase and realize immune reconstitution. Overall, LVs can perform significant roles in the treatment of primary immunodeficiency diseases, hemoglobinopathies, B cell leukemia, and neurodegenerative diseases. In this review, we discuss the recent developments and therapeutic applications of LVs. The safe and efficient LVs show great promise as a tool for human gene therapy. | Xiaoyu Wang Cuicui Ma Roberto Rodríguez Labrada Zhou Qin Ting Xu Zhiyao He Yuquan Wei | 2021 | Science China(Life Sciences)2021，64，11： | 4 |
| 14 | Type I CRISPR-Cas targets endogenous genes and regulates virulence to evade mammalian host immunity显示文摘定期聚类短 palindromic 重复的 interspaced (CRISPR ) 在细菌和 archaea 的 -CRISPR-associated (Cas ) 系统对入侵外国 nucleic 酸提供适应免疫。以前的研究建议某些细菌采用他们的类型 II CRISPR-Cas 系统指向他们的自己的基因,因此躲避主人免疫。然而,另外的 CRISPR-Cas 系统是否在主机房间的细菌的侵略期间有类似的函数,仍然保持未知。这里,我们为类型识别一个新奇角色我在在 Pseudomonas aeruginosa 躲避主人防卫的 CRISPR-Cas 系统拉紧 UCBPP-PA14。类型我 PA14 的 CRISPR-Cas 系统指向细菌的察觉到治安法官的管理者 LasR 的 mRNA 阻抑由像使用费的受体的识别 4,因此减少在房间和鼠标模型的主机的支持 inflammatory 回答。机械学地,这调停核酸酶的 RNA 降级在类型的 Cas3 在目标 mRNA,和 HD 和 DExD/H 领域要求一个 5-GGN-3 识别主题我 CRISPR-Cas 系统。作为 LasR 和类型我 CRISPR-Cas 系统在细菌是无所不在地在场的,我们的调查结果阐明重要普通机制内在的细菌的毒力。 | Rongpeng Li Lizhu Fang Shirui Tan Min Yu Xuefeng Li Sisi He Yuquan Wei Guoping Li Jianxin Jiang Min Wu | 2016 | Cell Research2016，26，12： | 4 |
| 15 | Personalized neoantigen pulsed dendritic cell vaccine for advanced lung cancer显示文摘Neoantigens are considered to be ultimate target of tumor immunotherapy due to their high tumor specificity and immunogenicity.Dendritic cell(DCs)vaccines based on neoantigens have exciting effects in treatment of some malignant tumors and are a promising therapeutic modality.Lung cancer is a lethal disease with the highest morbidity and mortality rate in the world.Despite the rapid development of targeted therapy and immune checkpoint inhibitors for lung cancer in recent years,their efficacy is still unsatisfactory overall.Therefore,there is an urgent unmet clinical need for lung cancer treatment.Here,we attempted to treat lung cancer using a personalized neoantigen peptide-pulsed autologous DC vaccine and conducted a single-arm,2 medical centers,pilot study initiated by the investigator(ChiCTR-ONC 16009100,NCT02956551).The patients enrolled were patients with heavily treated metastatic lung cancer.Candidate neoantigens were derived from whole-exome sequencing and RNA sequencing of fresh biopsy tissues as well as bioinformatics analysis.A total of 12 patients were enrolled in this study.A total of 85 vaccine treatments were administered with a median value of 5 doses/person(range:3-14 doses/person).In total,12-30 peptide-based neoantigens were selected for each patient.AIl treatment-related adverse events were grade 1-2 and there were no delays in dosing due to toxic effects.The objective effectiveness rate was 25%;the disease control rate was 75%;the median progression-free survival was 5.5 months and the median overall survival was 7.9 months.This study provides new evidence for neoantigen vaccine therapy and new therapeutic opportunities for lung cancer treatment. | Zhenyu Ding Qing Li Rui Zhang Li Xie Yang shu Song Gao Peipei Wang Xiaoqing Su Yun Qin Yuelan Wang Juemin Fang Zhongzheng Zhu Xuyang Xia Guochao Wei Hui Wang Hong Qian Xianling Guo Zhibo Gao Yu Wang Yuquan Wei Qing Xu Heng Xu Li Yang | 2021 | Signal Transduction and Targeted Therapy2021，6，2： | 3 |
| 16 | Author correction to‘Multifunctional regulatory protein connective tissue growth factor(CTGF):A potential therapeutic target for diverse diseases’[Acta pharmaceutica sinica B 12(2022)1740-1760]显示文摘The authors regret that there was an error in 7.Conclusion and prospect section of the article.The last paragraph of this section‘Under particular external…integrins interact with CT domain’is supposed to be the content of the figure legend(Fig.1).It is superfluous in this part and should be deleted.The revision does not affect any conclusions of this work. | Minyang Fu Dandan Peng Tianxia Lan Yuquan Wei Xiawei Wei | 2022 | Acta Pharmaceutica Sinica B2022，12，12： | 3 |
| 17 | A new and promising application of gene editing: CRISPR-controlled smart materials for tissue engineering,bioelectronics, and diagnostics显示文摘A recent study published in the journal Science by Collins et al.proposed a programmable clustered regularly interspaced short palindromic repeats(CRISPR)-responsive smart material containing the CRISPR-associated nuclease,Casl2a,and hydrogels containing DNA to deliver biological information via changes in material properties(English et al.,2019). | Weiqi Hong Mengyuan Huang Yuquan Wei Xiawei Wei | 2019 | Science China(Life Sciences)2019，62，11： | 3 |
| 18 | A novel cancer vaccine with the ability to simultaneously produce anti-PD-1 antibody and GM-CSF in cancer cells and enhance Th1-biased antitumor immunity显示文摘Tumor escape from immune-mediated destruction has been associated with immunosuppressive mechanisms that inhibit T-cell activation.A promising strategy for cancer immunotherapy is to disrupt key pathways regulating immune tolerance,such as program death-1(PD-1/PD-L1)pathway in the tumor environment.However,the determinants of response to anti-PD-1 monoclonal antibodies(mAbs)treatment remain incompletely understood.In murine models,PD-1 blockade alone fails to induce effective immune responses to poorly immunogenic tumors,but is successful when combined with additional interventions,such as cancer vaccines.Novel cancer vaccines combined with antibody may offer promising control of cancer development and progression.In this investigation,we generated a novel tumor cell vaccine simultaneously expressing anti-PD-1 mAbs and granulocyte-macrophage colony stimulating factor(GM-CSF)in CT26 colon cancer and B16-F10 melanoma.The antitumor effect of the vaccine was verified by therapeutic and adoptive animal experiments in vivo.The antitumor mechanism was analyzed using Flow cytometry,Elispot and in vivo intervention approaches.The results showed that tumor cell vaccine secreting PD-1 neutralizing antibodies and GM-CSF induced remarkable antitumor immune effects and prolonged the survival of tumor-bearing animals compared with animals treated with either PD-1 mAbs or GM-CSF alone.Antitumor effects and prolonged survival correlated with strong antigen-specific T-cell responses by analyzing CD11c^(+)CD86^(+)DC,CD11b^(+)F4/80^(+)MΦcells,increased ratio of Teff/Treg in the tumor microenvironment,and higher secretion levels of Th1 proinflammatory cytokines in serum.Furthermore,the results of ELISPOT and in vivo blocking strategies further confirmed that the antitumor immune response is acquired by CD4 and CD8 T immune responses,primarily dependent on CD4 Th1 immune response,not NK innate immune response.The combination of PD-1 blockade with GM-CSF secretion potency creates a novel tumor cell vaccine immunotherapy,affording significantly improved antitumor responses by releasing the state of immunosuppressive microenvironment and augmenting the tumor-reactive T-cell responses. | Hongwei Tian Gang Shi Qin Wang Yiming Li Qianmei Yang Chunlei Li Guoyou Yang Min Wu Qian Xie Shuang Zhang Yang Yang Rong Xiang Dechao Yu Yuquan Wei Hongxin Deng | 2016 | Signal Transduction and Targeted Therapy2016，1，1： | 3 |
| 19 | Structural basis of the Norrin-Frizzled 4 interaction显示文摘 | Guobo Shen Jiyuan Ke Zhizhi Wang Zhihong Cheng Xin Gu Yuquan Wei Karsten Melcher H Eric Xu Wenqing Xu | 2015 | Cell Research2015，25，9： | 3 |
| 20 | The structure differences of Japanese encephalitis virus SA14 and SA14-14-2 E proteins elucidate the virulence attenuation mechanism显示文摘Dear Editor,Japanese encephalitis(JE)is a mosquito-borne acute neurological infectious disease caused by the Japanese encephalitis virus(JEV).Globally,68,000 cases of the disease are estimated each year,with a fatality rate of as high as 30%and with approximately 30%-50%of survivors suffering from severe neurological sequelae(WHO,2015). | Xinyu Liu Xin Zhao Rui Na Lili Li Eberhard Warkentin Jennifer Witt Xu Lu Yongxin Yu Yuquan Wei Guohong Peng Yuhua Li Junzhi Wang | 2019 | Protein & Cell2019，10，2： | 3 |
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