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| 1 | Improved Base Editor for Efficiently Inducing Genetic Variations in Rice with CRISPR/Cas9- Guided Hyperactive hAID Mutant显示文摘 | Bin Ren Fang Yan Yongjie Kuang Na Li Dawei Zhang Xueping Zhou Honghui Lin Huanbin Zhou | 2018 | Molecular Plant2018，11，4： | 46 |
| 2 | Base-Editing-Mediated Artificial Evolution of OsALS1 In Planta to Develop Novel Herbicide-Tolerant Rice Germplasms显示文摘Recently developed CRISPR-mediated base editors,which enable the generation of num erous nucleotide changes in target genomic regions,have been widely adopted for gene correction and generation of crop germ plasms containing im portant gain-of-function genetic variations.How ever,to engineer target genes with unknown functional SNPs remains challenging.To address this issue,we present here abase-e diting-mediated gene evolution(BEMGE)m ethod,employing both Cas9n-based cytosine and adenine base editors as well as a single-guide RNA(sgRNA)library tiling the full-length coding region,for developing novel rice germ plasm swith mutations in any endogenous gene.To this end,OsALS1 was artificially evolved in rice cells using BEMGE through both Agrobacterium-mediated and particle-bom bardment-mediated transform ation.Four different types of amino acid substitutions in the evolved OsALS1,derived from two sites that have never been targeted by natural or human selection during rice dom estication,were identified,conferring varying levels of tolerance to the herbicide bispyribac-sodium.Furtherm ore,the P171F substitution identified in a strong OsALS1 allele was quickly introduced into the commercial rice cultivar Nangeng 46 through precise base editing w ith the corresponding base editor and sgRNA.Collectively,these data indicate great potential of BEMGE in creating important genetic variants of target genes for crop improvement. | Yongjie Kuang Shaofang Li Bin Ren Fang Yan Carl Spetz Xiangju Li Xueping Zhou Huanbin Zhou | 2020 | Molecular Plant2020，13，4： | 37 |
| 3 | A CRISPR/Cas9 toolkit for efficient targeted base editing to induce genetic variations in rice显示文摘Dear Editor,Gene-targeting technologies using sequence specific nucleases have been widely adopted to induce genetic modifications in both plant molecular biology research and crop improvement,of which generating targeted point mutation for gain-of-function phenotype is of great value.Although tremendous efforts have been made in developing geneinsertion or replacement approaches by | Bin Ren Fang Yan Yongjie Kuang Na Li Dawei Zhang Honghui Lin Huanbin Zhou | 2017 | Science China(Life Sciences)2017，60，5： | 28 |
| 4 | Highly Efficient A. T to G. C Base Editing by Cas9n- 3uided tRNA Adenosine Deaminase in Rice显示文摘 | Fang Yan Yongjie Kuang Bin Ren Jingwen Wang Dawei Zhang Honghui Lin Bing Yang Xueping Zhou Huanbin Zhou | 2018 | Molecular Plant2018，11，4： | 26 |
| 5 | Cas9-NG Greatly Expands the Targeting Scope of the Genome-Editing Toolkit by Recognizing NG and Other Atypical PAMs in Rice显示文摘CRISPR technologies enabling precise genome manipulation are valuable for gene function studies and molecular crop breeding. However, the requirement of a protospacer adjacent motif (PAM)y such as NGG and TTN, for Cas protein recognition restricts the selection of targetable genomic loci in practical applications of CRISPR technologies. Recently Cas9-NG, which recognizes a minimal NG PAM, was reported to expand the targeting space of genome editing in human cells, but it remains unclear whether this Cas9 variant can be used in plants. In this study, we evaluated the nuclease activity of Cas9-NG toward various NGN PAMs by targeting endogenous genes in transgenic rice. We found that Cas9-NG edits all NGG, NGA, NGT, and NGC sites with impaired activity, while the gene-edited plants were dominated by monoallelic mutations. Cas9-NG-engineered base editors were then developed and used to generate O s B Z R I gainof- function plants that can not be created by other available Cas9-engineered base editors. Moreover, we showed that a Cas9-NG-based transcriptional activator efficiently upregulated the expression of endogenous target genes in rice. In addition, we discovered that Cas9-NG recognizes NAC, NTG, NTT, and NCG apart from NG PAM. Together, these findings demonstrate that Cas9-NG can greatly expand the targeting scope of genome-editing tools, showing great potential for targeted genome editing, base editing, and genome regulation in plants. | Bin Ren Lang Liu Shaofang Li Yongjie Kuang Jingwen Wang Dawei Zhang Xueping Zhou Honghui Lin Huanbin Zhou | 2019 | Molecular Plant2019，12，7： | 18 |
| 6 | Synergistic immunoreaction of acupuncture-like dissolving microneedles containing thymopentin at acupoints in immune-suppressed rats显示文摘Dissolving microneedles carried drug molecules can effectively penetrate the stratum corneum of skin to improve the transdermal drug delivery. The traditional Chinese medicine acupuncture is based on the needle stimulation at a specific location(acupoint) to generate and transmit biochemical and physiological signals which alter the pathophysiological state of patients. However, the pain associated with conventional acupuncture needles and the requirement of highly trained professionals limit the development of acupuncture in non-Asian countries. The purpose of this study is to investigate whether the dissolving microneedles can be utilized as a self-administered painless replacement for acupuncture and locally released drug molecules can achieve expected therapeutic outcomes. Immunosuppressive rats were treated with acupuncture at Zusanli(ST36) acupoint using microneedles containing thymopentin.The immune functions and psychological mood of the immunosuppressed animals were examined. The proliferation of splenocytes was examined by CCK-8 assay. CD4 and CD8 expression patterns in spleen cells were detected by flow cytometry. The current study showed that use of either microneedles containing thymopentin or conventional acupuncture both resulted in immune cell proliferation, which was confirmed by flow cytometry. Furthermore, either conventional acupuncture or microneedles were able to effectively mitigate the anxiety caused by immune-suppression when applied on the ST36. | Qian Zhang Chuncao Xu Shiqi Lin Huanbin Zhou Gangtao Yao Hu Liu Lili Wang Xin Pan Guilan Quan Chuanbin Wu | 2018 | Acta Pharmaceutica Sinica B2018，8，3： | 9 |
| 7 | High-efficiency and multiplex adenine base editing in plants using new TadA variants显示文摘Recently reported adenine base editors(ABEs)exhibit powerful potential for targeted gene correction as well as developing gain-of-function mutants and novel germplasms for both gene function studies and crop breeding.However,editing efficiency varies significantly among different target sites.Here,we investigated the activities of three evolved E.coli adenosine deaminase TadA variants(TadA8e,TadA8.17,and TadA8.20)side-by-side in transgenic rice.We found that TadA8e outperforms TadA8.17 and TadA8.20,and induces efficient A-to-G conversion at all tested sites in the rice genome,including those that were un-editable by ABE7.10 in our previous experiments.Furthermore,V82S/Q154R mutations were incorporated into TadA8e,resulting in a new variant that we named TadA9.Our data show that TadA9 is broadly compatible with CRISPR/SpCas9,CRISPR/SpCas9-NG,and CRISPR/SpRY,as well as CRISPR/ScCas9 nickase systems,achieving comparable or enhanced editing in a larger editing window at diverse PAM sites as compared with TadA8e.Finally,TadA9 was used to simultaneously install novel SNPs in four endogenous herbicide target genes in the commercial rice cultivar Nangeng 46 for potential field application in.weed control.Collectively,we successfully generated a series of novel ABEs that can efficiently edit adenosines in the rice genome.Our findings suggest that TadA9 and TadA8e have great potentials in the development of plant base editors and crop molecular breeding. | Daqi Yan Bin Ren Lang Liu Fang Yan Shaofang Li Guirong Wang Wenxian Sun Xueping Zhou Huanbin Zhou | 2021 | Molecular Plant2021，14，5： | 8 |
| 8 | Warm temperature compromises JA-regulated basal resistance to enhance Magnaporthe oryzae infection in rice显示文摘Changes in global temperatures profoundly affect the occurrence of plant diseases.It is well known that rice blast can easily become epidemic in relatively warm weather.However,the molecular mechanism remains unclear.In this study,we show that enhanced blast development at a warm temperature(22C)compared with the normal growth temperature(28C)is rice plant-determined.Comparative transcriptome analysis revealed that jasmonic acid(JA)biosynthesis and signaling genes in rice could be effectively induced by Magnaporthe oryzae at 28C but not at 22C.Phenotypic analyses of the osaoc1 and osmyc2 mutants,OsCOI1 RNAi lines,and OsMYC2-OE plants further demonstrated that compromised M.oryzaeinduced JA biosynthesis and signaling lead to enhanced blast susceptibility at the warm temperature.Consistent with these results,we found that exogenous application of methyl jasmonate served as an effective strategy for improving blast resistance under the warm environmental conditions.Furthermore,decreased activation of JA signaling resulted in the downregulated expression of some key basal resistance genes at 22C when compared with 28C.Among these affected genes,OsCEBiP(chitin elicitorbinding protein precursor)was found to be directly regulated by OsMYB22 and its interacting protein OsMYC2,a key component of JA signaling,and this contributed to temperature-modulated blast resistance.Taken together,these results suggest that warm temperature compromises basal resistance in rice and enhances M.oryzae infection by reducing JA biosynthesis and signaling,providing potential new strategies for managing rice blast disease under warm climate conditions. | Jiehua Qiu Junhui Xie Ya Chen Zhenan Shen Huanbin Shi Naweed I.Naqvi Qian Qian Yan Liang Yanjun Kou | 2022 | Molecular Plant2022，15，4： | 5 |
| 9 | An investigation into the three-dimensional cloud structure over East Asia from the CALIPSO-GOCCP Data显示文摘The spatial distribution of clouds and their seasonal variations, and the three-dimensional(3D) cloud structures over East Asia have been analyzed with the CALIPSO-GOCCP data during the period from 2007 to 2012. The results show that there is a large cloud fraction greater than 0.7 over southern China, and the largest cloud fraction appears in southwest China. Besides, a large cloud fraction occurs over the southeast of the Tibetan Plateau. The total and high cloud fractions show notable variations with seasons, while the middle and low cloud fractions vary a little. As for cloud vertical structure, significant differences of the cloud vertical distributions are observed between over land and ocean. Cloud fractions and the height of the maximum cloud fractions decline gradually with the increasing latitude, except for the vertical-latitude profiles over the Tibetan Plateau regions. The longitude-vertical cross sections show similar patterns from the longitude 70° E to 140° E, except the profiles with large cloud fractions over the Tibetan Plateau. From the horizontal distribution patterns and vertical structures of the clouds over East Asia, it is concluded that the huge terrain of the Tibetan Plateau has significant impacts on the cloud formation over the Tibetan Plateau region and the areas to the east. At last, the clouds from the CALIPSO-GOCCP observations are compared to those from the ERA-Interim reanalysis data. The results indicate that the ERA-Interim reanalysis data provide reasonable spatial distribution patterns and the vertical structures in terms of the total cloud fraction over East Asia. However, the total cloud fraction was underestimated about 20% by the ERA-Interim reanalysis data over most parts of East Asia, especially over the neighboring areas east of the Tibetan Plateau. Additionally, the ERA-interim reanalysis data overestimate the cloud fractions at each level in the vertical direction. | YIN JinFang WANG DongHai XU HuanBin ZHAI GuoQing | 2015 | Science China Earth Sciences2015，58，12： | 4 |
| 10 | Rock paper scissors: CRISPR/Cas9-mediated interference with geminiviruses in plants显示文摘Geminiviruses are a group of circular single-stranded DNA viruses that constitute the largest family of plant viruses. Many diseases resulting from geminivirus infections, such as maize streak disease, cassava mosaic disease, tomato yellow leaf curl disease and cotton leaf curl diseases, cause significant problems in terms of economic losses, posing a serious threat to global crop productivity (Hanley-Bowdoin et al., 2013;Yang et al., 2019). As obligate intracellular parasites, geminiviruses cannot be controlled chemically. Conventional strategies to control geminiviruses rely heavily on the application of pesticides to limit insect vector populations, but it causes environmental concerns. To date, developing novel crop germplasms with durable disease resistance to geminiviruses is still the best strategy to manage viral infections due to its environmental friendliness. However, traditional breeding programs are time-intensive and labor-consuming processes, and transgene-based modern approaches by overexpressing the geminiviral proteins (e.g., the truncated form of the replication initiator protein) failed to confer resistance to geminiviruses due to activated virusinduced transgene silencing. | Xiuling Yang Huanbin Zhou Xueping Zhou | 2019 | Science China(Life Sciences)2019，62，10： | 4 |
| 11 | Bone marrow and the control of immunity显示文摘Bone marrow is thought to be a primary hematopoietic organ. However, accumulated evidences demonstrate that active function and trafficking of immune cells, including regulatory T cells, conventional T cells, B cells, dendritic cells, natural killer T (NKT) cells, neutrophils, myeloid-derived suppressor cells and mesenchymal stem cells, are observed in the bone marrow. Furthermore, bone marrow is a predetermined metastatic location for multiple human tumors. In this review, we discuss the immune network in the bone marrow. We suggest that bone marrow is an immune regulatory organ capable of fine tuning immunity and may be a potential therapeutic target for immunotherapy and immune vaccination. | Ende Zhao Huanbin Xu Lin Wang Ilona Kryczek Ke Wu Yu Hu Guobin Wang Weiping Zou | 2012 | Cellular & Molecular Immunology2012，9，1： | 3 |
| 12 | Chemical composition of the ore and occurrence state of the elements in Jingbaoshan platinum-palladium deposit显示文摘The Jingbaoshan platinum-palladium deposit is China’s largest independent PGM (platinum-group met- als) deposit so far discovered. There are eleven kinds of useful components in the ore: Pt, Pd, Os, Ir, Ru, Rh, Au, Ag, Cu, Ni, and Co. The platinum-group elements, gold and silver occur in the form of minerals in ores. twenty-five kinds of precious metal minerals have been found, of which twenty one belong to the platinum-group minerals. The minerals are very small in grain size. Copper occurs mainly as copper sulfide with a small amount of free copper oxide, and the beneficiated copper accounts for 95.21%. Nickel occurs mainly as nickel sulfide, and some nickel silicate and nickel oxide occur in the ore. The beneficiated nickel accounts for 72.03%. Cobalt occurs mainly as co- balt sulfide, and there are some cobalt oxide and other kinds of cobalt. The beneficiated cobalt accounts for 77.58%. | SONG Huanbin HE Mingqin ZHANG Shangzhong YI Fenghuang | 2008 | Chinese Journal Of Geochemistry2008，27，1： | 3 |
| 13 | Conferring Resistance to Plant RNA Viruses with the CRISPR/CasRx System显示文摘Dear Editor,Clustered regularly interspaced short palindromic repeat(CRISPR)-associated system(Cas)is an adaptive immune system discovered in prokaryotic bacteria or archaea that can fend off invading nucleic acids.Because of its simplicity,high efficiency and versatility,CRISPR/Cas system-mediated genome editing has been widely applied in plant research and agricultural production. | Yongsen Cao Huanbin Zhou Xueping Zhou Fangfang Li | 2021 | Virologica Sinica2021，36，4： | 2 |
| 14 | Putative Phosphatase UvPsr1 Is Required for Mycelial Growth, Conidiation, Stress Response and Pathogenicity in Ustilaginonidea virens显示文摘Ustilaginoidea virens is the causal agent of rice false smut,which can be a highly destructive disease of rice.The plasma membrane phosphatase Psr1 proteins,which act as a regulator of the salinity stress response in yeast,are widely distributed across fungi,but their functional characterization is sketchy.In this study,we characterized the functions of Psr1 protein,UvPsr1,in U.virens.Analyses of the AUvpsr1 and its complementation strain showed that UvPsr1 is required for normal mycelial growth,conidiation and tolerance to oxidative,osmotic and cell wall stresses.When rice panicles were inoculated with the AUvpsr1 strains,no symptoms of false smut disease developed,showing that UvPSR1 also contributes to the pathogenicity of the fungus.The deletion mutant of UvPSR1 also appeared to produce a smaller titer of toxic compounds able to inhibit elongation of the germinated seeds.In conclusion,our results indicated that UvPsr1 is a new pathogenic factor of U.virens. | Xiong Meng Meng Shuai Qiu Jiehua Shi Huanbin Shen Xiangling Kou Yanjun | 2020 | Rice science2020，27，6： | 2 |
| 15 | Eliminate indeterminacies of independent component analysis for chemometrics显示文摘An improved method has been proposed to eliminate the indeterminacies of independent component analysis (ICA) for chemometrics. Following the arrangement of principal components analysis (PCA), the ICA mixing matrix is selected as signal content indexes, and ICA output are sorted and directed. After many times reputations, independent components (ICs) are paired according to the maximum correlation coefficient, and then the mean values of each IC substitutes the original ICs. This indicates that the ICA indeterminacies are eliminated. A simulation example is tested to validate this improvement. Finally, a set of experimental LC-MS data is processed without any prior knowledge or specific limitation and the results show that the improved ICA can directly separate the mixed signals in chemometrics, and it is simpler and more reasonable than the simple to use interactive self-modeling mixture analysis (SIMPLISMA). | Zhixiang Yao Kai Zhang Huanbin Liu Hui Su | 2008 | Progress in Natural Science:Materials International2008，18，8： | 2 |
| 16 | Study on remov-al of dissolved and colloidal substances in deinked pulp by dual-com-ponent system retention aids containing nanosized TiO2 colloid显示文摘 | XiaoquanChen Wenhao Shen Huanbin Liu | 2009 | Appita2009，62，5： | 1 |
| 17 | Relationships between physical properties of brown rice and degree of milling and loss of selenium显示文摘 | Kunlun Liu Xiaohong Cao Qingyun Bai Huanbin Wen Zhenxin Gu | 2009 | Journal of Food Engineering2009，，1： | 1 |
| 18 | Effects of compo- nents in the culture solution on pepfides accumulation during germina- tion of brown rice显示文摘 | WEN Huanbin CAO Xiaohong GU Zhenxin | 2009 | Eur Food Res Technol2009，228，6： | 1 |
| 19 | Study on remov- al of dissolved and colloidal substances in deinked pulp by dual-com- ponent system retention aids containing nanosized TiO2 colloid 显示文摘 | Chen Xiaoquan Shen Wenhao Liu Huanbin | 2009 | Appita2009，62，5： | 1 |
| 20 | Differences in functional properties and biochemical characteristics of congeneric rice proteins显示文摘 | Xiaohong Cao Huanbin Wen Cuijuan Li | 2009 | Journal of Cereal Science2009，50，： | 1 |
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