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| 1 | Manipulating mRNA splicing by base editing in plants显示文摘Precursor-mRNAs(pre-mRNA) can be processed into one or more mature m RNA isoforms through constitutive or alternative splicing pathways. Constitutive splicing of pre-mRNA plays critical roles in gene expressional regulation, such as intronmediated enhancement(IME), whereas alternative splicing(AS) dramatically increases the protein diversity and gene functional regulation. However, the unavailability of mutants for individual spliced isoforms in plants has been a major limitation in studying the function of mRNA splicing. Here, we describe an efficient tool for manipulating the splicing of plant genes. Using a Cas9-directed base editor, we converted the 5′ splice sites in four Arabidopsis genes from the activated GT form to the inactive AT form. Silencing the AS of HAB 1.1(encoding a type 2 C phosphatase) validated its function in abscisic acid signaling, while perturbing the AS of RS31 A revealed its functional involvement in plant response to genotoxic treatment for the first time. Lastly,altering the constitutive splicing of Act2 via base editing facilitated the analysis of IME. This strategy provides an efficient tool for investigating the function and regulation of gene splicing in plants and other eukaryotes. | Chenxiao Xue Huawei Zhang Qiupeng Lin Rong Fan Caixia Gao | 2018 | Science China(Life Sciences)2018，61，11： | 18 |
| 2 | Shortening the sgRNA-DNA interface enables SpCas9 and eSpCas9(1.1)to nick the target DNA strand显示文摘The length of the sgRNA-DNA complementary sequence is a key factor influencing the cleavage activity of Streptococcus pyogenes Cas9(SpCas9)and its variants.The detailed mechanism remains unknown.Here,based on in vitro cleavage assays and base editing analysis,we demonstrate that reducing the length of this complementary region can confer nickase activity on SpCas9 and eSpCas9(1.1).We also show that these nicks are made on the target DNA strand.These properties encouraged us to develop a dual-functional system that simultaneously carries out double-strand DNA cleavage and C-to-T base conversions at separate targets.This system provides a novel tool for achieving trait stacking in plants. | Rong Fan Zhuangzhuang Chai Sinian Xing Kunling Chen Fengti Qiu Tuanyao Chai Jin-Long Qiu Zhengbin Zhang Huawei Zhang Caixia Gao | 2020 | Science China(Life Sciences)2020，63，11： | 2 |
| 3 | Ethyl pyruvate inhibits proliferation and induces apoptosis of hepatocellular carcinoma via regulation of the HMGB1–RAGE and AKT pathways显示文摘 | Ping Cheng Weiqi Dai Fan Wang Jie Lu Miao Shen Kan Chen Jingjing Li Yan Zhang Chengfen Wang Jing Yang Rong Zhu Huawei Zhang Yuanyuan Zheng Chuan-Yong Guo Ling Xu | 2013 | Biochemical and Biophysical Research Communications2013，，： | 1 |
| 4 | Diagnostic Performance of Des- γ -carboxy Prothrombin for Hepatocellular Carcinoma: A Meta-Analysis显示文摘 | Rong Zhu Jing Yang Ling Xu Weiqi Dai Fan Wang Miao Shen Yan Zhang Huawei Zhang Kan Chen Ping Cheng Chengfen Wang Yuanyuan Zheng Jingjing Li Jie Lu Yingqun Zhou Dong Wu Chuanyong Guo Fabio Farinati | 2014 | Gastroenterology Research and Practice2014，，： | 1 |
| 5 | Protective Effect of Astaxanthin on Liver Fibrosis through Modulation of TGF- β 1 Expression and Autophagy显示文摘 | Miao Shen Kan Chen Jie Lu Ping Cheng Ling Xu Weiqi Dai Fan Wang Lei He Yan Zhang Wang Chengfen Jingjing Li Jing Yang Rong Zhu Huawei Zhang Yuanyuan Zheng Yingqun Zhou Chuanyong Guo Sunil Kumar Manna | 2014 | Mediators of Inflammation2014，，： | 1 |
| 6 | Wüstite-type Fe0.78Mn0.22O nanocubes:A new class for high-sensitive T2-weighted magnetic resonance imaging agent显示文摘Wüstite-type Fe(0.78)Mn(0.22)O nanocubes,with a uniform size of^10 nm in edge length,have been synthesized by thermal-decomposition approach.The nanocubes exhibited superparamagnetic properties at room temperature,associated with a magnetization of 12.6 emu/g.These Fe(0.78)Mn(0.22)O nanocubes present transversal(r2)and longitudinal(r1)relaxivities of 325.9 and 0.518 mM^-1 s^-1 at 7 T for water protons.The ratio of the r2/r1(629.2)ranks them being the highest sensitivity(r2/r1)comparable to currently reported T2-weighted magnetic resonance imaging(MRI)agents.Meanwhile,the Fe(0.78)Mn(0.22)O nanocubes were functionalized and demonstrated to be biocompatible when attached to the surface of mesenchymal stem cells,therefore showing the promise as a new class of MRI agents in clinic applications. | Huawei Rong Haihua Hu Jian Zhang Jianjun Wang Mu Zhang Gaowu Qin Yanhui Zhang Xuefeng Zhang | 2019 | Journal of Materials Science & Technology2019，35，11： | 0 |
| 7 | Fe_(3)O_(4)@silica nanoparticles for reliable identification and magnetic separation of Listeria monocytogenes based on molecular-scale physiochemical interactions显示文摘Listeria monocytogenes(L.monocytogenes)is one of the top five dangerous foodborne pathogens which widely exists in most raw food and has approximately 30%mortality rate in high-risk groups.Food safety caused by foodborne pathogens is still a major problem faced by humans in all world.The conventional analytical methods currently used involve complex bacteriological tests and usually take several days for incubation and analysis.Thus,in order to prevent the spread of disease,the development of a detection method with high speed,high accuracy and sensitivity is urgent and necessary.Herein,we developed an approach for the identification and magnetic capture of L.monocytogenes by using core@shell Fe_(3)O_(4)@silica nanoparticles terminated with hydroxyl or amine groups.Our results show that both amine-and hydroxyl-terminated Fe_(3)O_(4)@silica core@shell nanoparticles functionalized with specific antibodies,present 95.2%±6.2%and 98.6%±0.3%capture efficacies,respectively.However,without conjugating the specific antibodies,the hydroxyl-terminated Fe_(3)O_(4)@silica nanoparticles exhibit 17.6%±1.6%efficacy,while the amine-terminated one remains 93.2%±9.2%capture efficiency ascribed to the high affinity.This study quantitatively uncovers the specific and non-specific recognitions relevant to the molecular-scale physiochemical interactions between the microorganisms and the functionalized particles,and the results from this work can be generalized and extended to other bacterial species by changing antibodies,also have important implications in developing advanced analytic methods. | Huawei Rong Tong Gao Yun Zheng Lingwei Li Dake Xu Xuefeng Zhang Yanglong Hou Mi Yan | 2021 | Journal of Materials Science & Technology2021，，25： | 0 |
| 8 | Preparation and Microwave Absorbing Properties of Double-layer Fine Iron Tailings Cementitious Materials显示文摘To develop the microwave absorbing(MA)properties of cementitious material mixed with mine solid waste,the iron tailings cementitious microwave absorbing materials were prepared.The iron tailings was treated into different particle sizes by planetary ball mill,and the physicochemical properties of iron tailings were tested by laser particle size analyzer and scanning electron microscope(SEM).The electromagnetic parameters of iron tailings cementitious materials were characterized by a vector network analyzer and simulated MA properties,and the MA properties of iron tailings-cement composite system with steel fiber as absorber was studied.Based on the design of the single-layer structure,optimum mix ratio and thickness configuration method of double-layer structure were further studied,meanwhile,the mechanical properties and engineering application were analyzed and discussed.The results show that the particle size of iron tailings can afiect its electromagnetic behavior in cementitious materials,and the smaller particles lead the increase of demagnetisation efiect induced by domain wall motion and achieve better microwave absorbing properties in cementitious materials.When the thickness of matching layer and absorbing layer is 5 mm,the optimized microwave absorbing properties of C1/C3 double-layer cementitious material can obtain optimal RL value of-27.61 dB and efiective absorbing bandwidth of 0.97 GHz,which attributes to the synergistic efiect of impedance matching and attenuation characteristics.The double-layer microwave absorbing materials obtain excellent absorbing properties and show great design flexibility and diversity,which can be used as a suitable candidate for the preparation of favorable microwave absorbing cementitious materials. | LI Huawei WANG Rong WANG Yulin LIU Feiyu WANG Qian WEI Muwang | 2023 | Journal of Wuhan University of Technology(Materials Science)2023，38，5： | 0 |
| 9 | Solid-state reaction induced defects in multi-walled carbon nanotubes for improving microwave absorption properties显示文摘Dielectric polarization performance induced by defect engineering approach has been proved to be an effective way for improving the microwave absorption property of carbon-based materials.Assisting by the solid-state reaction,the structural integrity of multi-walled carbon nanotubes(MWCNTs)would be broken along with the volume expansion and etching process of cobalt oxides.Therefore,the defects could be obtained and result in the enhancement of microwave absorption property.Ascribing to the broken wall structures,the defect-distances(L_(D))and concentrations(n_(D))have been optimized to be 9.80 nm and3.37×10^(11) cm^(-2).The minimum reflection loss(RL)had reached-54.6 dB at 4.5 GHz with a thickness of4.13 mm and the corresponding effective absorption bandwidth(EAB_(<-10 dB))was analyzed to be 14.6 GHz.Such enhancement is correlated to the dielectric polarization and the permeability-to-permittivity transformation raised from the defect structures and concentrations.The present work demonstrates an effective strategy for tailoring the microwave absorption property of MWCNTs by engineering defect concentrations,and could be further extended to other carbon-based absorbents. | Tong Gao Zhengyu Zhang Yixing Li Yujuan Song Huawei Rong Xuefeng Zhang | 2022 | Journal of Materials Science & Technology2022，，13： | 0 |
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