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| 1 | A Highly Pathogenic Strain of Porcine Deltacoronavirus Caused Watery Diarrhea in Newborn Piglets显示文摘Porcine deltacoronavirus(PDCoV) is a newly identified virus that causes watery diarrhea in newborn piglets and results in significant economic losses to the pig industry. Since first reported in Hong Kong in 2012, PDCoV has been subsequently detected in USA, South Korea, Thailand, and China's Mainland. Here we isolated a strain of PDCoV, named CHN-GD-2016,from the intestinal content of a diseased newborn piglet with severe diarrhea in a pig farm in Guangdong, China. PDCoV CHN-GD-2016 could be identified by immunofluorescence with PDCoV specific rabbit antisera, and typical crown-shaped particles with spiky surface projections of this PDCoV were observed with electron microscopy. Genomic analysis showed that the PDCoV CHN-GD-2016 was closely related to other Chinese PDCoV strains, with the highest sequence similarity with the strain CHN/Tianjin/2016. Importantly, inoculation of newborn piglets with 1×10~5 TCID_(50) of CHN-GD-2016 by oral feeding successfully reproduced clear clinical symptoms, including vomiting, dehydration, and severe diarrhea in piglets. In addition, the virus RNA in rectal swabs from 1 to 7 days post inoculation was detected, macroscopic and microscopic lesions in small intestine were observed, and viral antigen was also detected in the small intestines with immunohistochemical staining. Collectively, the data show in this study confirms that PDCoV is present in Guangdong,China and is highly pathogenic in newborn piglets. | Zhichao Xu Huiling Zhong Qingfeng Zhou Yunping Du Li Chen Yun Zhang Chunyi Xue Yongchang Cao | 2018 | Virologica Sinica2018，33，2： | 16 |
| 2 | Self-Powered Implantable Skin-Like Glucometer for Real-Time Detection of Blood Glucose Level In Vivo显示文摘Implantable bioelectronics for analyzing physiological biomarkers has recently been recognized as a promising technique in medical treatment or diagnostics. In this study, we developed a self-powered implantable skinlike glucometer for real-time detection of blood glucose level in vivo. Based on the piezo-enzymatic-reaction coupling effect of GOx@ZnO nanowire, the device under an applied deformation can actively output piezoelectric signal containing the glucose-detecting information. No external electricity power source or battery is needed for this device, and the outputting piezoelectric voltage acts as both the biosensing signal and electricity power. A practical application of the skin-like glucometer implanted in mouse body for detecting blood glucose level has been simply demonstrated. These results provide a new technique path for diabetes prophylaxis and treatment. | Wanglinhan Zhang Linlin Zhang Huiling Gao Wenyan Yang Shuai Wang Lili Xing Xinyu Xue | 2018 | Nano-Micro Letters2018，10，2： | 9 |
| 3 | Mechanical properties of irradiated multi-phase polycrystalline BCC materials显示文摘Structure materials under severe irradiations in nuclear environments are known to degrade because of irradiation hardening and loss of ductility,resulting from irradiation-induced defects such as vacancies,interstitials and dislocation loops,etc.In this paper,we develop an elastic-viscoplastic model for irradiated multi-phase polycrystalline BCC materials in which the mechanical behaviors of individual grains and polycrystalline aggregates are both explored.At the microscopic grain scale,we use the internal variable model and propose a new tensorial damage descriptor to represent the geometry character of the defect loop,which facilitates the analysis of the defect loop evolutions and dislocation-defect interactions.At the macroscopic polycrystal scale,the self-consistent scheme is extended to consider the multiphase problem and used to bridge the individual grain behavior to polycrystal properties.Based on the proposed model,we found that the work-hardening coefficient decreases with the increase of irradiation-induced defect loops,and the orientation/loading dependence of mechanical properties is mainly attributed to the different Schmid factors.At the polycrystalline scale,numerical results for pure Fe match well with the irradiation experiment data.The model is further extended to predict the hardening effect of dispersoids in oxide-dispersed strengthened steels by the considering the Orowan bowing.The influences of grain size and irradiation are found to compete to dominate the strengthening behaviors of materials. | Dingkun Song Xiazi Xiao Jianming Xue Haijian Chu Huiling Duan | 2015 | Acta Mechanica Sinica2015，31，2： | 4 |
| 4 | A nanocleaner specifically penetrates the blood-brain barrier at lesions to clean toxic proteins and regulate inflammation in Alzheimer's disease显示文摘Insurmountable blood-brain barrier(BBB) and complex pathological features are the key factors affecting the treatment of Alzheimer's disease(AD).Poor accumulation of drugs in lesion sites and undesired effectiveness of simply reducing Aβ deposition or TAU protein need to be resolved urgently.Herein,a nanocleaner is designed with a rapamycin-loaded ROS-responsive PLGA core and surface modification with KLVFF peptide and acid-cleavable DAG peptide [R@(ox-PLGA)-KcD].DAG can enhance the targeting and internalization effect of nanocleaner towards neurovascular unit endothelial cells in AD lesions,and subsequently detach from nanocleaner in response to acidic microenvironment of endosomes to promote the transcytosis of nanocleaner from endothelial cells into brain parenchyma.Then exposed KLVFF can capture and carry Aβ to microglia,attenuating Aβ-induced neurotoxicity.Strikingly,rapamycin,an autophagy promoter,is rapidly liberated from nanocleaner in the high ROS level of lesions to improve Aβ degradation and normalize inflammatory condition.This design altogether accelerates Aβ degradation and alleviates oxidative stress and excessive inflammatory response.Collectively,our finding offers a strategy to target the AD lesions precisely and multi-pronged therapies for clearing the toxic proteins and modulating lesion microenvironment,to achieve efficient AD therapy. | Ting Lei Zhihang Yang Xue Xia Yuxiu Chen Xiaotong Yang Rou Xie Fan Tong Xiaolin Wang Huile Gao | 2021 | Acta Pharmaceutica Sinica B2021，11，12： | 4 |
| 5 | Drug Susceptibility Test of Volatile Oil of Artemisiaargyi to Avian Pathogenic Escherichia coli显示文摘The volatile oil of Artemisia argyi was extracted by ultrasonic assisted extraction, and the extraction rate of volatile oil was 0.68%. The volatile oil of A. argyi was emulsified with 1% Tween-80, and drug susceptibility test was conducted with avian Escherichia coli. The results showed that the volatile oil of A. argyi had antibacterial effect against avian E. coli, and the minimal inhibitory concentration was 50 mg/mL. Taking six common antibiotics as the control, drug susceptibility test was conducted with volatile oil of A. argyi. The results showed that 10 strains of E. coli were sensitive to the volatile oil of A. argyi, three of which had different degrees of resistance and one had the tendency of resistance. | pan baiming he caimei liang changxiang zhou huiling li xue | 2018 | Animal Husbandry and Feed Science2018，10，4： | 1 |
| 6 | Massive electronic records processing for digital archives in cloud 显示文摘 | ZHANG Guigang XUE Sixin FENG Huiling | 2012 | Lecture Notes in Computer Science2012，7719，： | 1 |
| 7 | Honeycomb-Like Hydrogel Microspheres for 3D Bulk Construction of Tumor Models显示文摘A two-dimensional(2D)cell culture-based model is widely applied to study tumorigenic mechanisms and drug screening.However,it cannot authentically simulate the threedimensional(3D)microenvironment of solid tumors and provide reliable and predictable data in response to in vivo,thus leading to the research ilusionis and failure of drug screening.In this study,honeycomb-like gelatin methacryloyl(GelMA)hydrogel microspheres are developed by synchronous photocrosslinking microluidic technique to construct a 3D model of osteosarcoma.The in vitro study shows that ostcosarcoma cells(K7M2)culured in 3D GelMA microspheres have stronger tumorous stemness。proliferation and migration abilitics,more osteoclastogenetic ability,and reistance to chemotherapeutic drugs(DOX)than that of cells in 2D cultures.More imporantly,the 3D-cultured K7M2 cells show more tumorigenicity in immunologically sound mice,characterized by shorter tumorigenesis time,larger tumor volumc,severe bone destruction,and higher mortality.In conclusion,honeycomb like porous microsphere scaffolds are constructed with uniform structure by micofluidic technology to massively produce tumor cells with original phenotypes.Those microspheres could recapitulate the physiology microenvironment of tumors.maintain ell-cell and cell-extracellular matrix interactions,and thus provide an efective and convenient strategy for tumor pathogenesis and drug screening research. | Jiachen He Chichi Chen Liang Chen Ruoyu Cheng Jie Sun Xingzhi Liu Lin Wang Can Zhu Sihan Hu Yuan Xue Jian Lu Huiling Yang Wenguo Cui Qin Shi | 2022 | Research2022，，2： | 1 |
| 8 | Identification of the proliferation/differentiation switch in the cellular network of multicel- lular organisms显示文摘 | Xia Kai Xue Huiling Dong Dong | 2006 | PLoS Computational Biol2006，2，11： | 1 |
| 9 | Numerical simulation of cavitating flow around a slender body with slip boundary condition显示文摘In this paper, we perform a numerical simulation of the cavitating flow around an underwater hemispherical-head slender body running at a high speed. For the first time, the slip boundary condition is introduced into this problem, and we find that the slip boundary condition has a big influence on the cavitation in the flow-separation zone. By simulating the cavitating flow under different cavitation numbers, we demonstrate that the slip boundary condition can effectively reduce the intensity of cavitation, as represented by the length of cavitation bubbles. The present paper provides a new method for utilization of new surface materials to control the cavitation on the underwater moving objects. | Hao Liu DanDan Li YaHui Xue PengYu Lu YiPeng Shi HuiLing Duan | 2016 | Science China(Physics,Mechanics & Astronomy)2016，59，2： | 0 |
| 10 | A DNA G-quadruplex converts SOD1 into fibrillar aggregates显示文摘Nucleic acids with G4 elements play a role in the formation of aggregates involved in intracellular phase transitions.Our previous studies suggest that different forms of DNA could act as an accelerating template in Cu/Zn superoxide dismutase(SOD1)aggregation.Here,we examined the regulation of fo rmation and cytotoxicity of the SOD1 aggregates by single-stranded 12-merdeoxynucleotide oligomers(dN)_(12)(N=A,T,G,C;ssDNAs)under acidic conditions.The ssDNAs can be divided into two groups based on their roles in SOD1 binding,exposure of hydrophobic clusters in SOD1,accelerated formation,morphology and cytotoxicity of SOD1 aggregates.G-quadruplexes convert SOD1 into fibrillar aggregates as a template,a fact which was observed for the first time in the nucleic acid regulation of protein aggregation.Moreover,the fibrillar or fibril-like SOD1 species with a G-quadruplex provided by(dG)_(12)were less toxic than the amorphous species with(dN)_(12)(N=A,T).This study not only indicates that both morphology and cytotoxicity of protein aggregates can be regulated by the protein-bound DNAs,but also help us understand roles of nucleic aid G-quadruplexes in the formation of aggregates and membrane less organelles involved in intracellular phase transitions. | Wenqian Liu Yulin Xu Xue Li Yan Meng Huiling Wang Chunrong Liu Changlin Liu Li Wang | 2021 | Chinese Chemical Letters2021，32，7： | 0 |
| 11 | Lattice-distorted Pt wrinkled nanoparticles for highly effective hydrogen electrocatalysis显示文摘Modulating Pt surfaces through the introduction of lattice distortion emerges as immensely effective strategy that enhances the kinetics of alkaline hydrogen evolution and oxidation processes.In this study,we fabricated lattice-distorted Pt wrinkled nanoparticles(LD-Pt WNPs)for efficient hydrogen electrocatalysis.The LD-Pt WNPs not only outperform the Pt/C benchmark in hydrogen oxidation reaction,achieving an excellent mass-specific current of 968.5 mA·mg_(Pt)^(-1)(9 times that of Pt/C),but also demonstrate outstanding hydrogen evolution reaction activity with a small overpotential of 58.0 mV.Comprehensive experiments and density functional theory calculations reveal that lattice defects introduce an abundance of unsaturated coordination atoms while modifying the d-band center of Pt.This dual effect optimizes the binding strength of crucial H and OH intermediates,leading to a significant reduction in the energy barrier of the reaction bottleneck,commonly known as the Volmer step.This work unveils a fresh viewpoint on projecting and developing high efficiency electrocatalysts through defect engineering. | Xue Li Xiang Han Zhenrui Yang Shun Wang Yun Yang Juan Wang Jiadong Chen Zhongwei Chen Huile Jin | 2024 | Nano Research2024，17，5： | 0 |
| 12 | Author correction to “A nanocleaner specifically penetrates the blood‒brain barrier at lesions to clean toxic proteins and regulate inflammation in Alzheimer’s disease” [Acta Pharmaceutica Sinica B 12, (2021) 4032e4044]显示文摘The authors regret that there were some picture errors in Fig.4A and data errors Fig.7A owing to the mistakes of copying and pasting in the process of assembling figures and making a dia-gram.The raw data of correct images of group b in Fig.4A and values of high dose in Fig.7A are provided in the supporting information,and the authors revise Figs.4 and 7 as below.The revisions do not affect any results and conclusions of the current work.The authors apologize for any inconvenience caused to the journal and readers. | Ting Lei Zhihang Yang Xue Xia Yuxiu Chen Xiaotong Yang Rou Xie Fan Tong Xiaolin Wang Huile Gao | 2022 | Acta Pharmaceutica Sinica B2022，12，6： | 0 |
| 13 | VIBRATION OF CANTILEVERS WITH ROUGH SURFACES显示文摘We study the effect of surface roughness on the resonance frequency of micro-cantilever sensors.The analysis demonstrates that surface roughness can enhance,decrease or even annul the effect of surface stress on the resonance frequency,depending on the surface inclination angle and the Poisson ratio of the coating film on the cantilever. | Huiling Duan Yahui Xue Xin Yi | 2009 | Acta Mechanica Solida Sinica2009，22，6： | 0 |
| 14 | Tolerance and acceptance of hepatic venous pressure gradient measurement in cirrhosis(CHESS1904):An international multicenter study显示文摘Aim:To determine the tolerance and acceptance of hepatic venous pressure gradient(HVPG)measurements in patients with liver cirrhosis.Methods:This prospective international multicenter study included 271 patients with cirrhosis who were scheduled to undergo HVPG measurement between October 2019 and June 2020.Data related to the tolerance and acceptance of HVPG measurements were collected using descriptive questionnaires.Results:HVPG measurements were technically successful in all 271 patients,with 141(52.0%)undergoing HVPG measurement alone.The complication rate was 0.4%.Postoperative pain was significantly lower than preoperative expected pain(p<0.001)and intraoperative pain(p<0.001),and intraoperative pain was also significantly lower than preoperative expected pain(p=0.036).No,mild,moderate,severe,and intolerable discomfort scores were reported by 36.9%,44.6%,11.1%,6.3%,and 0.4%of these patients,respectively,during HVPG measurement and by 54.6%32.5%,11.4%,1.5%,and 0%,respectively,after HVPG measurement.Of these patients,39.5%had little understanding and 10%had no understanding of the value of HVPG measurement,with 35.1%and 4.1%regarding HVPG measurements as being of little or no help,respectively.Most patients reported that they would definitely(15.5%),probably(46.9%),or possibly(29.9%)choose to undergo additional HVPG measurements again,and 62.7%regarded the cost of the procedure as acceptable.Conclusion:HVPG measurement was safe and well‐tolerated in patients with cirrhosis,but patient education and communication are warranted to improve the acceptance of this procedure. | Jun‐Hui Sun He Zhao Haijun Zhang Lei Li NecatiÖrmeci Zi‐Niu Yu Xun Li Shuangxi Li Xujun Yang Huaping Wei Xiaoliang Zhu Zhengcong Zhang Yajin Wang Zhongwei Zhao Jianting Mao Qiaohong Wu Xiaole Sun Huiling Xiang Kefeng Jia Chao Yang Wei Wu Xiuqing Lin Haixin Yao Changzeng Zuo Jitao Wang Bo Zhang Chunqing Zhang Xiaoling Wu Guangchuan Wang Shengjuan Yao Ruihang Wang Li Zhou Hui Huan Qingli Tu Xue Pu Feng Zhang Qin Yin Linpeng Zhang Ying Guo Jian Wang Kohei Kotani Sawako Uchida‐Kobayashi Norifumi Kawada He Zhu Li Li Wei Wang Guo Zhang Lei Yu Xudong Cui Qingliang Zhu Hailong Zhang Xiaoli Hu Rafael OXimenes Adriano Gonçalves de Araújo Giulliano Gardenghi Yubao Zheng Zebin Wu Mingsheng Huang Xiaoyong Chen Jun Wu Feng Xie Yang Bo Shengjuan Hu Linke Ma Xiao Li Xiaolong Qi | 2022 | Portal Hypertension & Cirrhosis2022，1，1： | 0 |
| 15 | Combination losartan with hyaluronic acid modified diethyldithiocarbamate loaded hollow copper sulfide nanoparticles for the treatment of breast cancer and metastasis显示文摘The application of photothermal therapy(PTT)is greatly limited by the low accumulation of photothermal agents,uneven photothermal distribution,and heat endurance of cancer cells.Worse still,despite PTT enhances immunogenicity,the anti-tumor immune efficacy is still unsatisfactory due to the inefficient immunogenic cell death(ICD)induction and poor infiltration of immune cells.To solve the above problems of PTT,we developed hyaluronic acid(HA)modified hollow copper sulfide nanoparticles encapsulating diethyldithiocarbamate(DDTC)to construct a breast tumor targeting and near infrared(NIR)photo-responsive drug delivery system(D-HCuS@HA),which further combined with losartan to improve the accumulation and penetration in the tumor site.Upon irradiation,D-HCuS@HA realized enhanced PTT and released cytotoxic Cu(DDTC)_(2)to eliminate heat endurance tumor cells,thereby enhancing antitumor effect and inducing effective ICD.Moreover,the combination with losartan could remodel the tumor microenvironment,allowing more T cells to infiltrate into the tumor,and significantly inhibiting the occurrence and development of metastatic tumors.In vitro/vivo results revealed the great potential of D-HCuS@HA combined with losartan,which provides a new paradigm for anti-tumor and anti-metastases. | Haili Hu Weiwei Zhang Lei Lei Fan Tong Huilin Zhang Yiwei Zhang Wenqin Yang Yilu Tang Ruyi Lin Xue Xia Jiamei Li Shiyong Song Huile Gao | 2024 | Chinese Chemical Letters2024，35，3： | 0 |
| 16 | Study on the clinical significance of TRPV2 and MMP2 expressions in ovarian cancer显示文摘Ovarian cancer was one of the most common malignant tumors in female reproductive organs.Moreover,epithelial ovarian cancer showed the highest mortality rate in gynecological tumors,posing serious threats to women’s life and health.Transient Receptor Potential Cation Channel Subfamily V Member 2(TRPV2)and matrix metalloproteinase-2 MMP-2 have been found to play important roles in regulating the pathogenesis of most tumors,but there were few studies exploring the relationships of TRPV2 and MMP-2 in OC.Therefore,we evaluated the expression of TRPV2 and MMP-2 proteins in cancer tissues and adjacent normal tissues of OC patients.Immunohistochemistry was used to analyze the expressions of TRPV2 and MMP-2 in cancer tissues(N=70)and adjacent normal tissues(N=70)of OC patients,and the correlation of TRPV2 and MMP-2 with the occurrence and development of OC was analyzed with the combination of clinicopathological parameters of OC patients.The results showed that the expressions of TRPV2 and MMP-2 in OC tissues were significantly higher than those in adjacent normal tissues,and there were significant differences in TRPV2 and MMP-2 expressions in terms of tumor stage,differentiation,and lymph node metastasis of OC.Taken together,our results showed that protein expressions of TRPV2 and MMP-2 were closely related associated with the occurrence and development of OC. | XIAOPANG REN WANJIAO HAO JIN LIU YE LI BING WANG XINHUI ZU HUILING XUE | 2021 | BIOCELL2021，45，3： | 0 |
| 17 | Developmental systems biology flourishing on new technologies显示文摘Organism development is a systems level process. It has benefited greatly from the recent technological advances in the field of systems biology. DNA microarray, phenome, interactome and transcriptome mapping, the new generation of deep sequencing technologies, and faster and better computational and modeling approaches have opened new frontiers for both systems biologists and developmental biologists to reexamine the old developmental biology questions, such as pattern formation, and to tackle new problems, such as stem cell reprogramming. As showcased in the International Developmental Systems Biology Symposium organized by Chinese Academy of Sciences, developmental systems biology is flourishing in many perspectives, from the evolution of developmental systems, to the underly-ing genetic and molecular pathways and networks, to the genomic, epigenomic and noncoding levels, to the computational analysis and modeling. We believe that the field will continue to reap rewards into the future with these new approaches. | Jing-Dong J. Han Yi Liu Huiling Xue Kai Xia Hong Yu Shanshan Zhu Zhang Chen Wei Zhang Zheng Huang Chunyu Jin Bo Xian Jing Li Lei Hou Yixing Han Chaoqun Niu Timothy C. Alcon | 2008 | Journal of Genetics and Genomics2008，35，10： | 0 |
| 18 | Light Intensity Affects the Coloration and Structure of Chimeric Leaves of Ananas comosus var.bracteatus显示文摘Ananas comosus var.bracteatus is an important ornamental plant because of its green/white chimeric leaves.The accumulation of anthocyanin makes the leaf turn to red especially in the marginal part.However,the red fades away in summer and winter.Light intensity is one of the most important factors affecting leaf color along the seasons.In order to understand the effects of light intensity on the growth and coloration of the chimeric leaves,Ananas comosus var.bracteatus was grown under full sunlight,50%shade and 75%shade for 75 days to evaluate the concentration of pigments,the color parameters(values L^(*),a^(*),b^(*))and the morpho-anatomical variations of chimeric leaves.The results showed that a high irradiance was beneficial to keep the chimeric leaves red.However,prolonged exposure to high irradiance caused a damage,some of the leaves wrinkled and even burned.Shading instead decreased the concentration of anthocyanin and increased the concentration of chlorophyll,especially in the white marginal part of the leaves.Numerous chloroplasts were observed in the mesophyll cells of the white marginal part of the chimeric leaves under shading for 75 days.The increase in chlorophyll concentration resulted in a better growth of plants.In order to balance the growth and coloration of the leaves,approximately 50%shade is suggested to be the optimum light irradiance condition for Ananas comosus var.bracteatus in summer. | Wei Yang Yuke Lin Yanbin Xue Meiqin Mao Xuzixing Zhou Hao Hu Jiawen Liu Lijun Feng Huiling Zhang Jiaheng Luo Jun Ma | 2022 | Phyton-International Journal of Experimental Botany2022，91，2： | 0 |
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